Veterinary Sciences最新文献_第3页

Synergistic Enhancement of Humoral and Cellular Immunity in Attenuated Live Vaccines Using Chemical Nanoadjuvants: Evaluation of Sustained-Release Formulations. 化学纳米佐剂协同增强减毒活疫苗的体液和细胞免疫：缓释制剂的评价

IF 2.3 2区农林科学

Veterinary Sciences Pub Date : 2025-09-17 DOI: 10.3390/vetsci12090899

Jianli Shi, Chang Liu, Chen Li, Jun Li

{"title":"Synergistic Enhancement of Humoral and Cellular Immunity in Attenuated Live Vaccines Using Chemical Nanoadjuvants: Evaluation of Sustained-Release Formulations.","authors":"Jianli Shi, Chang Liu, Chen Li, Jun Li","doi":"10.3390/vetsci12090899","DOIUrl":"10.3390/vetsci12090899","url":null,"abstract":"Achieving a balance between enhancing both humoral and cellular immunity while ensuring sustained release for attenuated live vaccines has long been a challenge in the vaccine industry. However, the incorporation of chemical materials as nanoadjuvants has emerged as a promising strategy to address this challenge. In this study, a novel nano-adjuvant with sustained release properties was developed by combining polyethylene glycol (PEG) and other biochemical and molecular biology agents with CpG. The efficacy of this adjuvant was assessed using the widely applied CSF attenuated live vaccine. The body temperature and relative daily weight gain results demonstrated the safety of the formulations. Importantly, the combination of CpG immune enhancers and sustained-release agents led to a synergistic augmentation of both humoral and cellular immune responses. Specifically, the combination of PEG20000 and benzoic acid, along with Seppic white oil, significantly enhanced humoral immunity. In contrast, the combination of total white oil, PEG6000 and benzoic acid demonstrated superior efficacy in promoting cellular immunity. This study offers important insights into enhancing vaccine efficacy through novel adjuvant combinations, which can be applied to the development of more effective vaccines in the future.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statistical Triage Model for Feline Infectious Diseases in a Veterinary Isolation Unit: The Case of Feline Immunodeficiency and Leukemia Viruses. 兽医隔离单位中猫传染病的统计分类模型：猫免疫缺陷病毒和白血病病毒的案例。

IF 2.3 2区农林科学

Veterinary Sciences Pub Date : 2025-09-17 DOI: 10.3390/vetsci12090902

Miguel M Maximino, Inês C Machado, Telmo P Nunes, Luís M Tavares, Virgílio S Almeida, Solange A Gil, Nuno Sepúlveda

{"title":"Statistical Triage Model for Feline Infectious Diseases in a Veterinary Isolation Unit: The Case of Feline Immunodeficiency and Leukemia Viruses.","authors":"Miguel M Maximino, Inês C Machado, Telmo P Nunes, Luís M Tavares, Virgílio S Almeida, Solange A Gil, Nuno Sepúlveda","doi":"10.3390/vetsci12090902","DOIUrl":"10.3390/vetsci12090902","url":null,"abstract":"Isolation units in veterinary hospitals are essential for managing infectious diseases, but limited resources in staff, equipment and budget make timely triage difficult. In this scenario, data-driven models, if trained with high-quality data and presenting good accuracy, might be a rapid and low-cost first step before more costly diagnostics are deployed to unclear cases. In this study, we aimed at constructing simple triage models for two common feline infections using routine-collected data from 1211 cats admitted to a Biological Isolation and Containment Unit (BICU) in Portugal, of which 640 were eligible for analysis. Our study focused on infections caused by Feline Immunodeficiency and Leukemia viruses (FIV and FeLV) due to their potential to induce immunosuppression and increased susceptibility to secondary diseases. Our model training was based on data from 134 FIV-seropositive cats, 126 FeLV-seropositive, and 504 confirmed non-cases (i.e., controls) of these infections diagnosed by rapid immunomigration assays and ELISA. Significant triage factors of FIV infections included older age, outdoor access, and concomitant disorders whereas, for FeLV, infections, they were mixed-breed status, concomitant conditions, and low hematocrit. The models showed areas under the receiver operating characteristic curve of 0.70 and 0.69 for FIV and FeLV infections, respectively, with sensitivities and specificities estimated above ≥65%. This performance, being far from sensitivities and specificities above 90%, motivates further research to find additional triage factors to improve model utility.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Risk Factors Associated with Treatment for BRD in Beef Calves Within the First 60 Days After Arrival at Fattening Operations in Northwestern Italy Beef Calves. 意大利西北部肉牛育肥作业后60天内与肉牛BRD治疗相关的风险因素识别。

IF 2.3 2区农林科学

Veterinary Sciences Pub Date : 2025-09-16 DOI: 10.3390/vetsci12090898

Isabella Nicola, Giuliano Borriello, Edoardo Ramacciotti, Giovanni Gallina, Maurizio Beltramo, Claudio Bellino

{"title":"Identification of Risk Factors Associated with Treatment for BRD in Beef Calves Within the First 60 Days After Arrival at Fattening Operations in Northwestern Italy Beef Calves.","authors":"Isabella Nicola, Giuliano Borriello, Edoardo Ramacciotti, Giovanni Gallina, Maurizio Beltramo, Claudio Bellino","doi":"10.3390/vetsci12090898","DOIUrl":"10.3390/vetsci12090898","url":null,"abstract":"Bovine respiratory disease (BRD) impacts beef cattle health and farming systems. To better understand the BRD predisposing factors, a study was conducted in northwest Italy on a population of 26 batches (760 animals) of beef cattle imported from France. Of these, 173 underwent physical examination for clinical signs of BRD (rectal temperature > 39.5 °C, respiratory rate > 36 bpm, cough, nasal or ocular discharge) and blood sampling to detect antibodies against bovine viral respiratory infectious agents (e.g., parainfluenza 3, bovine herpesvirus type 1), haptoglobin and reactive oxygen metabolites concentrations, on arrival. Data on BRD treatments performed within 60 days of arrival were extracted from farm registers. The two most frequent cattle breeds were Blonde d'Aquitaine (80/173, 46.2%) and Limousine (61/173, 35.3%); the median batch weight was 332 kg (range, 195-470). At least one clinical sign of BRD was noted in 57.2% (99/173) of the animals. Most animals tested positive for BPIV3 (131/173, 75.7%) and BRSV (112/172, 64.7%). Bovine respiratory disease treatment was associated with transport time and average weight on arrival. Moreover, reactive oxygen metabolites levels differed between treated and untreated animals; this difference could help predict the onset of BRD, though further studies are needed to draw conclusions.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Macrophage and Granulocytic Recruitment with Increased Neo-Angiogenesis in Chicken Embryo Yolk Sac Following In Ovo Probiotic Blend Administration. 蛋蛋混合益生菌促进鸡胚卵黄囊巨噬细胞和粒细胞募集，增加新生血管生成。

IF 2.3 2区农林科学

Veterinary Sciences Pub Date : 2025-09-15 DOI: 10.3390/vetsci12090892

Lucia Biagini, Stefano Pesaro, Livio Galosi, Donatella Volpatti, Danilo De Bellis, Alessandra Roncarati, Alessandra Gavazza, Giacomo Rossi

{"title":"Enhanced Macrophage and Granulocytic Recruitment with Increased Neo-Angiogenesis in Chicken Embryo Yolk Sac Following In Ovo Probiotic Blend Administration.","authors":"Lucia Biagini, Stefano Pesaro, Livio Galosi, Donatella Volpatti, Danilo De Bellis, Alessandra Roncarati, Alessandra Gavazza, Giacomo Rossi","doi":"10.3390/vetsci12090892","DOIUrl":"10.3390/vetsci12090892","url":null,"abstract":"The yolk sac (YS) plays a pivotal role in avian embryonic development, contributing to both haematopoiesis and immune maturation. This study aimed to evaluate the effects of in ovo administration of a commercial probiotic blend (Slab51®) on YS cellular dynamics in chicken embryos. At embryonic day (ED) 18, Ross308 broiler eggs were injected with either the probiotic suspension (P) or sterile saline solution (C). YS tissues were sampled at 8, 12, 24 and 36 h post-inoculation for histological and immunohistochemical analysis. Probiotic-treated embryos exhibited a significant reduction in granulocytic foci within the YS, potentially reflecting enhanced peripheral migration of mature granulocytes. Concurrently, a progressive increase in Iba-1+ macrophages was observed in the probiotic group, suggesting accelerated macrophage differentiation. Immunophenotyping revealed a predominance of M1-iNOS+ macrophages across all timepoints, although a significant increase in M2-CD204+ macrophages was detected at 36 h in probiotic-treated embryos, indicating a possible anti-inflammatory shift. Furthermore, a marked increase in CD31+ endothelial cells in the probiotic group supports an associated rise in neo-angiogenesis. These findings suggest that in ovo probiotic administration modulates the YS microenvironment by promoting early macrophage recruitment, macrophage polarization and vascular remodeling. To our knowledge, this is the first report to demonstrate probiotic-induced structural and immunological alterations in the chicken embryo YS. These results provide novel insights into the early immunomodulatory effects of probiotics and highlight the potential of the YS as a key mediator of host-probiotic interaction during embryogenesis.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association Between FABP3 and FABP4 Genes with Changes in Milk Composition and Fatty Acid Profiles in the Native Southern Yellow Cattle Breed. 南方土种黄牛FABP3和FABP4基因与乳成分和脂肪酸谱变化的关系

IF 2.3 2区农林科学

Veterinary Sciences Pub Date : 2025-09-15 DOI: 10.3390/vetsci12090893

Mervan Bayraktar, Serap Göncü, Atalay Ergül, Recep Karaman, Bahri Devrim Özcan, Şerife Ergül, Celile Aylin Oluk, Özgül Anitaş, Ahmet Bayram, Mohammed Baqur S Al-Shuhaib

{"title":"Association Between FABP3 and FABP4 Genes with Changes in Milk Composition and Fatty Acid Profiles in the Native Southern Yellow Cattle Breed.","authors":"Mervan Bayraktar, Serap Göncü, Atalay Ergül, Recep Karaman, Bahri Devrim Özcan, Şerife Ergül, Celile Aylin Oluk, Özgül Anitaş, Ahmet Bayram, Mohammed Baqur S Al-Shuhaib","doi":"10.3390/vetsci12090893","DOIUrl":"10.3390/vetsci12090893","url":null,"abstract":"Fatty acid binding proteins FABP3 and FABP4 act as intracellular lipid chaperones that influence fatty acid transport and metabolism in mammary tissue, and genetic variation in these genes may affect milk composition. We examined the associations between FABP3 and FABP4 polymorphisms and milk composition and fatty acid profiles in 200 lactating Native Southern Yellow (NSY) cows. DNA from each cow was PCR-amplified and Sanger-sequenced for FABP3 and FABP4; genotypes were tested for their association with milk fatty acid concentrations and standard composition traits using linear models adjusted for relevant covariates. We detected a missense variant in FABP3 (c.3656G > A; p.Val45Met) and an intronic SNP in FABP4 (g.3509T > C). The FABP3 p.Val45Met AA genotype was associated with higher concentrations of butyric, palmitic, oleic, and α-linolenic acids. Cows with the FABP4 TC genotype exhibited elevated levels of myristoleic, γ-linolenic, conjugated linoleic, and arachidic acids, along with increased fat-free dry matter, protein, and lactose. In silico analyses provided mixed evidence for the structural effects of p.Val45Met, molecular docking suggested altered ligand affinity for several fatty acids, and splice site prediction implicated g.3509T > C in possible transcript processing changes. These variants constitute candidate markers for milk fatty acid composition in NSY cattle; replication in independent cohorts and functional validation are recommended to confirm their utility for milk quality improvement.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Immunization Against Inhibin on Camel Testicular Morphometry, Echotexture Analysis, Semen Quality, and Vascularization in Relation to Hormonal Aspect. 免疫抑制素对骆驼睾丸形态、回声分析、精液质量和与激素有关的血管化的影响。

IF 2.3 2区农林科学

Veterinary Sciences Pub Date : 2025-09-15 DOI: 10.3390/vetsci12090896

Elshymaa A Abdelnaby, Hossam R El-Sherbiny, Abdulrhman K Alhaider, Mohamed Marzok, Ibrahim A Emam

{"title":"Effect of Immunization Against Inhibin on Camel Testicular Morphometry, Echotexture Analysis, Semen Quality, and Vascularization in Relation to Hormonal Aspect.","authors":"Elshymaa A Abdelnaby, Hossam R El-Sherbiny, Abdulrhman K Alhaider, Mohamed Marzok, Ibrahim A Emam","doi":"10.3390/vetsci12090896","DOIUrl":"10.3390/vetsci12090896","url":null,"abstract":"This study aims to demonstrate whether immunization against inhibin could alters testicular echotexture (TE), hemodynamics (resistance and pulstalilty index [RI and PI], testicular blood flow volume [TBFV]), and semen picture. Senile male camels were immunized actively against inhibin alpha subunit (immunized males; n = 5; subcutaneous route; 1 mL) or non immunized (control males; n = 5). The injection was four times with four weeks intervals. Semen was collected by electroejaculator once per week. Blood sampling, ultrasonography, and hormonal assaying was performed once per week. The levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), testosterone (T), and nitric oxide (NO) were calculated. Regarding control camels, the FSH levels were elevated (p < 0.01) in immunized males weeks 6-9 in August. Both E2 and NO were increased in the same group at weeks 7-11 with the highest level in week 10 for E2 (17.01 ± 0.11 pg/mL) in September and in week 9 for NO (44.66 ± 0.15 µmol/L). The Doppler indices RI and PI were declined (p < 0.01) in immunized camels in weeks 7-11, while the TBFV was elevated (p < 0.01). A marked elevation (p < 0.05) in testicular volume was noticed in the immunized group with a decline in the TE in week 9. The sperm cell concentration and viability were elevated (p < 0.01) in the immunized group. In conclusion, the active immunization against inhibin in senile camels shows a positive effect via improvement of testicular hemodynamics, flow volume, testicular volume, FSH, E2, NO, and sperm cell concentration with viability %, and alterations in both Doppler indices with the TE.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal Dynamics and Surveillance of Highly Pathogenic H5 Avian Influenza in Wild Birds in Northern Serbia (2016-2025). 塞尔维亚北部野生鸟类高致病性H5禽流感的时间动态和监测（2016-2025）

IF 2.3 2区农林科学

Veterinary Sciences Pub Date : 2025-09-15 DOI: 10.3390/vetsci12090894

Biljana Djurdjević, Milena Samojlović, Diana Lupulović, Tamaš Petrović, Vladimir Polaček, Slobodan Knežević, Marko Pajić

{"title":"Temporal Dynamics and Surveillance of Highly Pathogenic H5 Avian Influenza in Wild Birds in Northern Serbia (2016-2025).","authors":"Biljana Djurdjević, Milena Samojlović, Diana Lupulović, Tamaš Petrović, Vladimir Polaček, Slobodan Knežević, Marko Pajić","doi":"10.3390/vetsci12090894","DOIUrl":"10.3390/vetsci12090894","url":null,"abstract":"Wild birds, particularly migratory waterfowl (family Anatidae), are the primary reservoir of avian influenza viruses, playing a key role in their transboundary spread. In the winter of 2016/2017, the H5N8 strain was first recorded in Serbia, with 20 outbreaks confirmed in wild birds by 2021, mostly involving mute swans in the Vojvodina region. From 2021 onward, the dominant strain was H5N1, with 38 confirmed outbreaks up to 2023, primarily affecting mute swans, black-headed gulls, and common cranes. Sporadic H5N2 outbreaks were also detected, with two cases in 2021 and 2023. Due to its rich ornithofauna, natural reserves, and habitats suitable for migratory birds, the Vojvodina region has been officially designated as a high-risk zone by the Veterinary Directorate of the Ministry of Agriculture, Forestry and Water Management of the Republic of Serbia. While sporadic cases occurred in backyard poultry, commercial farms remained unaffected. Infections peaked in autumn and early spring. Control measures included enhanced biosecurity, movement restrictions, and removal of dead birds to protect domestic poultry. Despite the circulation of avian influenza virus in wild birds, Serbia's commercial poultry industry avoided outbreaks and major economic losses. Including outbreak data provided insight into the scale and persistence of avian influenza threats in this region.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Effectiveness of Single-Nucleotide Polymorphisms Versus Microsatellites for Parentage Verification in Horse Breeds. 单核苷酸多态性与微卫星在马品种亲子鉴定中的有效性评价。

IF 2.3 2区农林科学

Veterinary Sciences Pub Date : 2025-09-15 DOI: 10.3390/vetsci12090890

Dongsoo Kim, Sunyoung Lee, Baatartsogt Oyungerel, Giljae Cho

{"title":"Evaluation of the Effectiveness of Single-Nucleotide Polymorphisms Versus Microsatellites for Parentage Verification in Horse Breeds.","authors":"Dongsoo Kim, Sunyoung Lee, Baatartsogt Oyungerel, Giljae Cho","doi":"10.3390/vetsci12090890","DOIUrl":"10.3390/vetsci12090890","url":null,"abstract":"This study aimed to generate information for parentage testing in horse breeds using microsatellites (STRs) and single-nucleotide polymorphisms (SNPs). Genotype data were obtained from 189 horse hair root samples, including 38 Thoroughbreds (TBs), 17 Jeju horses (JHs), 20 Quarter horses (QHs), 21 American Miniatures (AMs), and 93 Mongolian horses (MHs), using 15 STR markers and 71 SNP markers. Comparative analysis revealed that the mean expected heterozygosity ranged from 0.468 (AM) to 0.491 (JH) for SNPs and from 0.695 (TB) to 0.791 (MH) for STRs. The mean observed heterozygosity ranged from 0.415 (AM) to 0.487 (MH) for SNPs and from 0.706 (JH) to 0.776 (MH) for STRs. The mean polymorphic information content ranged from 0.349 (AM) to 0.364 (MH) for SNPs and from 0.635 (TB) to 0.761 (MH) for STRs. The inbreeding coefficient ranged from -0.009 (MH) to 0.113 (AM) for SNPs and from -0.058 (TB) to 0.043 (AM) for STRs. The cumulative exclusion probability (PE) for the 71-SNP panel exceeded 0.9999, indicating that SNP markers may be sufficient for parentage testing. In comparison, the STR markers yielded a combined PE of 0.9988 when one parent was known and 0.9999 when both parents were known. These findings highlight the potential of SNPs as alternatives to STRs for routine paternity verification in horses.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Detection of Enterocytozoon bieneusi in Free-Range Sheep and Domestic Dogs from the Greater Hinggan Mountains Area of China. 大兴安岭地区散养羊和家犬中双胞虫的分子检测。

IF 2.3 2区农林科学

Veterinary Sciences Pub Date : 2025-09-15 DOI: 10.3390/vetsci12090897

Yanyan Jiang, Zhongying Yuan, Xu Wang, Hongling Zhang, Hao Zhou, Weiping Wu, Yujuan Shen, Jianping Cao

{"title":"Molecular Detection of Enterocytozoon bieneusi in Free-Range Sheep and Domestic Dogs from the Greater Hinggan Mountains Area of China.","authors":"Yanyan Jiang, Zhongying Yuan, Xu Wang, Hongling Zhang, Hao Zhou, Weiping Wu, Yujuan Shen, Jianping Cao","doi":"10.3390/vetsci12090897","DOIUrl":"10.3390/vetsci12090897","url":null,"abstract":"Enterocytozoon bieneusi (E. bieneusi) is a globally distributed microsporidian that infects both humans and animals. However, reports on its occurrence in free-ranging sheep and domestic dogs from rural areas are limited. The objective of this study was to investigate the prevalence and genotype distribution of E. bieneusi among freely grazing sheep and household dogs in the Hinggan Mountain region of China. A total of 235 fecal samples were collected, consisting of 95 from sheep and 140 from dogs, and screened for E. bieneusi by nested PCR targeting the internal transcribed spacer region (ITS) within the nuclear ribosomal DNA (rDNA) gene. The total infection rate of E. bieneusi amounted to 3.0% (7/235), with sheep exhibiting a rate of 5.3% (5/95) and dogs demonstrating a rate of 1.4% (2/140). Sequence analysis identified two known genotypes in sheep (BEB6 and NESH4, both within phylogenetic group 2) and one known genotype in dogs (CHN-F1, phylogenetic group 1), with CHN-F1 being reported in dogs for the first time. Importantly, the detection of BEB6, a genotype previously associated with human infection, suggests that sheep could act as a potential reservoir contributing to zoonotic transmission and environmental contamination. These findings expand knowledge of the genotype spectrum of E. bieneusi in rural animal populations and provide evidence of possible cross-species transmission between humans and the surveyed animals in the region.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Longitudinal Observational Study on Lactation-Associated Changes in Procalcitonin, Protein Carbonyl Content, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Dairy Cattle. 奶牛降钙素原、蛋白羰基含量、不对称二甲基精氨酸和对称二甲基精氨酸与泌乳期相关变化的纵向观察研究

IF 2.3 2区农林科学

Veterinary Sciences Pub Date : 2025-09-15 DOI: 10.3390/vetsci12090895

Giulia Sala, Matteo Castelli, Chiara Orsetti, Giovanni Armenia, Lucia De Marchi, Valentina Meucci, Micaela Sgorbini, Francesca Bonelli

{"title":"A Longitudinal Observational Study on Lactation-Associated Changes in Procalcitonin, Protein Carbonyl Content, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Dairy Cattle.","authors":"Giulia Sala, Matteo Castelli, Chiara Orsetti, Giovanni Armenia, Lucia De Marchi, Valentina Meucci, Micaela Sgorbini, Francesca Bonelli","doi":"10.3390/vetsci12090895","DOIUrl":"10.3390/vetsci12090895","url":null,"abstract":"Procalcitonin (PCT), protein carbonyl content (PCC), asymmetric dimethylarginine (ADMA), and symmetric dimethylarginine (SDMA) have been proposed as promising biomarkers for detecting diseases in cattle. Their concentrations could potentially be influenced by lactation due to oxidative stress commonly observed during this period. This study aimed to evaluate plasma levels of PCT, PCC, ADMA, and SDMA at different stages of lactation in 21 healthy dairy cows: at 15 (T0), 60 (T1), and 150 (T2) days in milk (DIM). Clinically healthy Italian Holstein-Friesian cows were included, selected based on healthy dry periods and weekly veterinary checks during lactation. Blood samples were collected at each time point and biomarkers were measured using validated analytical methods. Data were analyzed using Friedman's test and the p value was set at 0.05. Median (IQR) PCT values were 64.29 (40.00-143.23), 75.36 (40.00-161.47), and 77.50 pg/mL (40.00-120.18) at T0, T1, and T2, respectively. PCC medians were 0.17 (0.10-0.27), 0.14 (0.08-0.23), and 0.20 (0.08-0.22) nmol/mL/mg; ADMA values were 0.11 (0.09-0.15), 0.11 (0.09-0.13), and 0.10 µmol/L (0.09-0.14); and SDMA values were 0.11 (0.09-0.14), 0.12 (0.09-0.15), and 0.10 µmol/L (0.09-0.16). No statistically significant differences were observed between time points for any biomarker. These findings suggest that, despite physiological oxidative stress during lactation, these biomarkers remain stable in healthy cows. Therefore, establishing distinct reference ranges based on lactation stage may not be necessary.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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