Evaluation of the Effectiveness of Single-Nucleotide Polymorphisms Versus Microsatellites for Parentage Verification in Horse Breeds.

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Dongsoo Kim, Sunyoung Lee, Baatartsogt Oyungerel, Giljae Cho
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Abstract

This study aimed to generate information for parentage testing in horse breeds using microsatellites (STRs) and single-nucleotide polymorphisms (SNPs). Genotype data were obtained from 189 horse hair root samples, including 38 Thoroughbreds (TBs), 17 Jeju horses (JHs), 20 Quarter horses (QHs), 21 American Miniatures (AMs), and 93 Mongolian horses (MHs), using 15 STR markers and 71 SNP markers. Comparative analysis revealed that the mean expected heterozygosity ranged from 0.468 (AM) to 0.491 (JH) for SNPs and from 0.695 (TB) to 0.791 (MH) for STRs. The mean observed heterozygosity ranged from 0.415 (AM) to 0.487 (MH) for SNPs and from 0.706 (JH) to 0.776 (MH) for STRs. The mean polymorphic information content ranged from 0.349 (AM) to 0.364 (MH) for SNPs and from 0.635 (TB) to 0.761 (MH) for STRs. The inbreeding coefficient ranged from -0.009 (MH) to 0.113 (AM) for SNPs and from -0.058 (TB) to 0.043 (AM) for STRs. The cumulative exclusion probability (PE) for the 71-SNP panel exceeded 0.9999, indicating that SNP markers may be sufficient for parentage testing. In comparison, the STR markers yielded a combined PE of 0.9988 when one parent was known and 0.9999 when both parents were known. These findings highlight the potential of SNPs as alternatives to STRs for routine paternity verification in horses.

单核苷酸多态性与微卫星在马品种亲子鉴定中的有效性评价。
本研究旨在利用微卫星(STRs)和单核苷酸多态性(SNPs)为马品种亲子鉴定提供信息。采用15个STR标记和71个SNP标记,对189份马毛根样本进行基因型分析,其中纯种马38匹、济州马17匹、四分之一马20匹、美洲迷你马21匹、蒙古马93匹。结果表明,SNPs的平均期望杂合度为0.468 (AM) ~ 0.491 (JH), STRs的平均期望杂合度为0.695 (TB) ~ 0.791 (MH)。SNPs的平均杂合度为0.415 (AM) ~ 0.487 (MH), STRs的平均杂合度为0.706 (JH) ~ 0.776 (MH)。snp的平均多态性信息含量为0.349 (AM) ~ 0.364 (MH), str的平均多态性信息含量为0.635 (TB) ~ 0.761 (MH)。SNPs近交系数为-0.009 (MH) ~ 0.113 (AM), STRs近交系数为-0.058 (TB) ~ 0.043 (AM)。71-SNP组的累积排除概率(PE)超过0.9999,表明SNP标记可能足以进行亲子鉴定。相比之下,当父母一方已知时,STR标记的总PE为0.9988,当父母双方都已知时,其总PE为0.9999。这些发现突出了snp作为常规马父系鉴定替代str的潜力。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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