Veterinary Sciences最新文献

Sperm Functional Status: A Multiparametric Assessment of the Fertilizing Potential of Bovine Sperm. 精子功能状态：牛精子受精潜能的多参数评估。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-12-23 DOI: 10.3390/vetsci11120678

Timea Sarah Odinius, Mathias Siuda, Matthias Lautner, Claus Leiding, Stefan Neuner, Heinrich Bollwein, Eleni Malama

{"title":"Sperm Functional Status: A Multiparametric Assessment of the Fertilizing Potential of Bovine Sperm.","authors":"Timea Sarah Odinius, Mathias Siuda, Matthias Lautner, Claus Leiding, Stefan Neuner, Heinrich Bollwein, Eleni Malama","doi":"10.3390/vetsci11120678","DOIUrl":"10.3390/vetsci11120678","url":null,"abstract":"Sperm viability is routinely assessed for the quality control of cryopreserved bovine sperm batches but is not usually conclusive regarding their fertilizing potential. In this study, we investigated the fertility predictive value of bull sperm viability in combination with DNA integrity or the functional status of viable sperm. In addition to sperm viability, we flow cytometrically assessed the percentage of sperm with high DNA fragmentation index (%DFI) and the fraction of viable sperm with low intracellular Ca2+ content and functional mitochondria using the Sperm Chromatin Structure Assay and a five-color staining panel in 791 and 733 cryopreserved batches with non-return rate (NRR) records after ≥100 first services, respectively. Using linear mixed-effects models and conditional inference trees, we examined the potential of sperm viability combined with either DNA integrity or the functional status of viable sperm to predict the batch-specific NRR. Batches with a %DFI of ≤6.86% were more likely to have a NRR of >60%, whereas %DFI values of >6.86% were more likely to be associated with a 55-60% or lower NRR. Combining post-thaw viability with the functional status of viable sperm did not reliably predict the NRR of individual batches. Concluding, the incorporation of DNA integrity assessment can considerably improve sperm fertility prognostics.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shape of the Doppler Sonographic Systolic Blood Flow Profile of the Pulmonary Artery of Healthy Racing Pigeons and the Influence of Anesthesia. 健康赛鸽肺动脉多普勒超声收缩血流曲线的形状及麻醉的影响。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-12-23 DOI: 10.3390/vetsci11120679

Carolin Girard, Lajos Koy, Norbert Kummerfeld, Michael Pees, Michael Fehr, Marko Legler

{"title":"Shape of the Doppler Sonographic Systolic Blood Flow Profile of the Pulmonary Artery of Healthy Racing Pigeons and the Influence of Anesthesia.","authors":"Carolin Girard, Lajos Koy, Norbert Kummerfeld, Michael Pees, Michael Fehr, Marko Legler","doi":"10.3390/vetsci11120679","DOIUrl":"10.3390/vetsci11120679","url":null,"abstract":"There is scant information available about the blood flow of the pulmonary artery in avian cardiology. In human medicine, the shape of the Doppler sonographic blood flow profile of the pulmonary artery can be used to access the pressure conditions of the right heart. With this background, this study focused on the examination of the acceleration and deceleration phase of the pulsed-wave Doppler flow profile of the pulmonary artery of healthy racing pigeons. The results showed a significant difference between the pulmonary artery and aorta. The Doppler flow profile of the aorta was characterized by a short acceleration phase; however, the pulmonary flow profile revealed an acceleration similar to that of the deceleration phase. Neither profile changed considerably under the influence of heart rate changes and anesthesia with isoflurane. A negative correlation of the pulmonary acceleration phase with the left diastolic A wave and the systolic pulmonary blood flow velocities could be found. This indicates the influence of pressure changes in the heart on the flow profiles. The results of this study allow for the use of the shape of the Doppler sonographic blood flow profile of the pulmonary artery in the assessment of cardiovascular diseases in avian medicine, especially in racing pigeons.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subclinical Mastitis in Lacaune Sheep: Etiologic Agents, the Effect on Milk Characteristics, and an Evaluation of Infrared Thermography and the YOLO Algorithm as a Preprocessing Tool for Advanced Analysis. laacaune羊的亚临床乳腺炎：病因，对奶特性的影响，以及红外热像仪和YOLO算法作为高级分析预处理工具的评价。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-12-22 DOI: 10.3390/vetsci11120676

Marios Lysitsas, Georgios Botsoglou, Dimitris Dimitriadis, Sofia Termatzidou, Panagiota Kazana, Grigorios Tsoumakas, Constantina N Tsokana, Eleni Malissiova, Vassiliki Spyrou, Charalambos Billinis, George Valiakos

{"title":"Subclinical Mastitis in Lacaune Sheep: Etiologic Agents, the Effect on Milk Characteristics, and an Evaluation of Infrared Thermography and the YOLO Algorithm as a Preprocessing Tool for Advanced Analysis.","authors":"Marios Lysitsas, Georgios Botsoglou, Dimitris Dimitriadis, Sofia Termatzidou, Panagiota Kazana, Grigorios Tsoumakas, Constantina N Tsokana, Eleni Malissiova, Vassiliki Spyrou, Charalambos Billinis, George Valiakos","doi":"10.3390/vetsci11120676","DOIUrl":"10.3390/vetsci11120676","url":null,"abstract":"This study aimed to investigate the incidence of subclinical mastitis (SCM), the implicated pathogens, and their impact on milk quality in dairy sheep in Greece. Furthermore, we preliminarily evaluated infrared thermography and the application of AI tools for the early, non-invasive diagnosis of relevant cases. In total, 660 milk samples and over 2000 infrared thermography images were obtained from 330 phenotypically healthy ewes. Microbiological investigations, a somatic cell count (SCC), and milk chemical analyses were performed. Infrared images were analyzed using the FLIR Research Studio software (version 3.0.1). The You Only Look Once version 8 (YOLOv8) algorithm was employed for the automatic detection of the udder's region of interest. A total of 157 mammary glands with SCM were identified in 122/330 ewes (37.0%). The most prevalent pathogen was staphylococci (136/160, 86.6%). Considerable resistance was detected to tetracycline (29.7%), ampicillin (28.6%), and sulfamethoxazole-trimethoprim (23.6%). SCM correlated with high total mesophilic count (TMC) values and decreased milk fat, lactose, and protein content. A statistically significant variation (p < 0.001) was identified in the unilateral SCM cases by evaluating the mean temperatures of the udder region between the teats in the thermal images. Finally, the YOLOv8 algorithm was employed for the automatic detection of the udder's region of interest (ROI), achieving 84% accuracy in defining the ROI in this preliminary evaluation. This demonstrates the potential of infrared thermography combined with AI tools for the diagnosis of ovine SCM. Nonetheless, more extensive sampling is essential to optimize this diagnostic approach.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Insights into Porcine Bocavirus (PBoV) and Its Impact on the Economy and Public Health. 猪博卡病毒（PBoV）的最新发现及其对经济和公共卫生的影响。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-12-22 DOI: 10.3390/vetsci11120677

Jelena Prpić, Tomislav Keros, Margarita Božiković, Magda Kamber, Lorena Jemeršić

{"title":"Current Insights into Porcine Bocavirus (PBoV) and Its Impact on the Economy and Public Health.","authors":"Jelena Prpić, Tomislav Keros, Margarita Božiković, Magda Kamber, Lorena Jemeršić","doi":"10.3390/vetsci11120677","DOIUrl":"10.3390/vetsci11120677","url":null,"abstract":"Effective control of animal infectious diseases is crucial for maintaining robust livestock production systems worldwide. Porcine meat constitutes approximately 35-40% of global meat production with the largest producers being China and the European Union (EU). Emerging viral pathogens in swine, like porcine bocavirus (PBoV), have not garnered significant attention, leaving their pathogenic characteristics largely unexplored. This review aims to bridge this knowledge gap by conducting a comprehensive analysis of the existing literature on PBoV. We explore the virus's genome structure, discovery, classification, detection methods, pathogenesis, and its potential public health implications. Additionally, we discuss the distribution and economic impact of PBoV, which includes potential losses due to decreased productivity, increased veterinary costs, and trade restrictions. By highlighting the current state of knowledge, this review seeks to enhance the understanding of PBoV, thereby aiding in its prevention and control, and mitigating its economic impact on the swine industry.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Ganoderma lucidum Powder on the Growth Performance, Immune Organ Weights, Cecal Microbiology, Serum Immunoglobulins, and Tibia Minerals of Broiler Chickens. 灵芝粉对肉鸡生长性能、免疫器官重量、盲肠微生物学、血清免疫球蛋白和胫骨矿物质的影响

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-12-22 DOI: 10.3390/vetsci11120675

Arazay Avain, Md Abul Kalam Azad, Yaneisy García, Yanelys García, Yordan Martínez

{"title":"Effects of Ganoderma lucidum Powder on the Growth Performance, Immune Organ Weights, Cecal Microbiology, Serum Immunoglobulins, and Tibia Minerals of Broiler Chickens.","authors":"Arazay Avain, Md Abul Kalam Azad, Yaneisy García, Yanelys García, Yordan Martínez","doi":"10.3390/vetsci11120675","DOIUrl":"10.3390/vetsci11120675","url":null,"abstract":"A total of 640 one-day-old Cobb 500 MV × Cobb 500 FF mixed broilers were randomly assigned to one of four experimental treatments with four replicates per treatment and 40 birds per replicate for 32 days. The treatments consisted of a basal diet (control group), basal diet + 0.02% zinc bacitracin (AGP group), basal diet + 0.2% G. lucidum powder (GLP; 0.2% GLP group), and basal diet + 0.3% GLP (0.3% GLP group). The results showed that dietary 0.2% GLP supplementation increased body weight compared to the control and 0.3% GLP groups, and decreased feed conversion ratio (FCR) compared to the control group, during 19-32 days (p < 0.05). The feed intake was lower (p < 0.05) in both dietary GLP supplementation groups and the AGP group during 1-8 and 1-32 days compared to the control group. Additionally, the FCR was lower in the dietary GLP supplementation group (0.2%) and the AGP group (p < 0.05) compared to the control group. Moreover, the caeca of broiler chickens in the AGP and 0.2% GLP groups had a higher abundance of lactic acid bacteria (LAB). Supplementation of feed additives (AGP and GLP) increased the relative weight of the thymus, with no effect on the bursa of Fabricius and spleen. However, AGP supplementation decreased the serum IgM concentration, while supplementing a higher dose of GLP (0.3%) increased the ash content in the tibia. The findings indicate that 0.2% GLP is the recommended supplementation dose as a natural growth promoter to replace AGP in apparently normal chickens.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Insights into Acute Gastroenteritis in Cattle Caused by Bovine Rotavirus A and Bovine Coronavirus. 牛轮状病毒A和牛冠状病毒致牛急性胃肠炎的比较研究。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-12-21 DOI: 10.3390/vetsci11120671

Vjekoslava Kostanić, Valentina Kunić, Marina Prišlin Šimac, Marica Lolić, Tomislav Sukalić, Dragan Brnić

{"title":"Comparative Insights into Acute Gastroenteritis in Cattle Caused by Bovine Rotavirus A and Bovine Coronavirus.","authors":"Vjekoslava Kostanić, Valentina Kunić, Marina Prišlin Šimac, Marica Lolić, Tomislav Sukalić, Dragan Brnić","doi":"10.3390/vetsci11120671","DOIUrl":"10.3390/vetsci11120671","url":null,"abstract":"Acute gastroenteritis (AGE) in cattle significantly impacts the economy due to relatively high morbidity and mortality and decreased production. Its multifactorial nature drives its global persistence, involving enteric viruses, bacteria, protozoa, and environmental factors. Bovine Rotavirus A (BoRVA) and bovine coronavirus (BCoV) are among the most important enteric RNA viruses causing AGE in cattle. These viruses infect intestinal enterocytes, leading to cell damage and consequently to malabsorption and diarrhea. BoRVA primarily affects calves under 14 days old with gastrointestinal clinical signs, while BCoV affects all ages, causing gastrointestinal and respiratory distress. The economic impact of BoRVA and BCoV, along with their interspecies transmission potential, warrants attention. This concise review discusses the molecular structure, epidemiology, pathogenesis, clinical signs, diagnosis, treatment, and preventive measures of BoRVA and BCoV while providing a comparative analysis. By offering practical guidance on managing such viral infections in cattle, these comparative insights may prove valuable for veterinarians in clinical practice.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenium Enhances the Growth of Bovine Endometrial Stromal Cells by PI3K/AKT/GSK-3β and Wnt/β-Catenin Pathways. 硒通过PI3K/AKT/GSK-3β和Wnt/β-Catenin通路促进牛子宫内膜基质细胞生长

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-12-21 DOI: 10.3390/vetsci11120674

Junsheng Dong, Zi Wang, Fan Fei, Yeqi Jiang, Yongshuai Jiang, Long Guo, Kangjun Liu, Luying Cui, Xia Meng, Jianji Li, Heng Wang

{"title":"Selenium Enhances the Growth of Bovine Endometrial Stromal Cells by PI3K/AKT/GSK-3β and Wnt/β-Catenin Pathways.","authors":"Junsheng Dong, Zi Wang, Fan Fei, Yeqi Jiang, Yongshuai Jiang, Long Guo, Kangjun Liu, Luying Cui, Xia Meng, Jianji Li, Heng Wang","doi":"10.3390/vetsci11120674","DOIUrl":"10.3390/vetsci11120674","url":null,"abstract":"The bovine uterus is susceptible to bacterial infections after calving, particularly from Escherichia coli (E. coli), which often results in endometritis. Additionally, postpartum stress in cows can elevate cortisol levels in the body, inhibiting endometrial regeneration and reducing immune function, thereby further increasing the risk of infection. Selenium (Se) is a common feed additive in dairy farming, known for its anti-inflammatory and antioxidant effects. The aim of this study was to investigate the regulatory role of Se in the growth of bovine endometrial stromal cells (BESCs) under the conditions of LPS-induced inflammatory damage at high cortisol levels. BESCs were treated with 1, 2, 4 μM Se in combination with co-treatment of LPS and cortisol. The results indicated that LPS inhibited the cell viability and reduced the mRNA expression of CTGF, TGF-β1, and TGF-β3. Additionally, LPS increased apoptosis, hindered the cell cycle progression by blocking it in the G0/G1 phase, and suppressed the PI3K/AKT/GSK-3β and Wnt/β-catenin signaling pathways. Furthermore, increased concentrations of cortisol can exacerbate the impacts of LPS on cell proliferation and apoptosis. Conversely, the supplementation of Se promoted cell viability, increased the mRNA expression of TGF-β1 and TGF-β3, and enhanced cell cycle progression, while simultaneously repressing cell apoptosis as well as activating the PI3K/AKT/GSK-3β and Wnt/β-catenin signaling pathways. The above findings demonstrated that Se can promote cell proliferation, reduce cell apoptosis, and aid in the growth of BESCs damaged by LPS under high levels of cortisol. The potential mechanisms may be associated with the regulation of the PI3K/AKT/GSK-3β and Wnt/β-catenin signaling pathways.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Evaluation of Non-Antibiotic Growth Promoters for Food Animals. 食用动物非抗生素生长促进剂的开发与评价。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-12-21 DOI: 10.3390/vetsci11120672

Hanfei Wang, Hengji Zhao, Bocheng Tai, Simeng Wang, Awais Ihsan, Haihong Hao, Guyue Cheng, Yanfei Tao, Xu Wang

{"title":"Development and Evaluation of Non-Antibiotic Growth Promoters for Food Animals.","authors":"Hanfei Wang, Hengji Zhao, Bocheng Tai, Simeng Wang, Awais Ihsan, Haihong Hao, Guyue Cheng, Yanfei Tao, Xu Wang","doi":"10.3390/vetsci11120672","DOIUrl":"10.3390/vetsci11120672","url":null,"abstract":"The widespread utilization of antibiotic growth promoters (AGPs) boosts the growth rate of food animals and enhances human living standards. Nevertheless, it is accompanied by escalating antibiotic resistance. Consequently, there is an urgent demand to develop novel alternatives to growth promoters. The objective of this study was to develop a non-antibiotic growth promoter (NAGP) for augmenting the growth rate of food animals. The growth-promoting effect of plant-derived NAGPs was assessed in mice and broiler chickens, and its growth-promoting mechanism was initially investigated. The results reveal that a combination of hawthorn (also known as shanzha) and astragalus (also known as huangqi) extracts (SQ) enhanced the growth rate of mice both in vivo and in vitro, attributed to their significant capacity to promote muscle growth and improve immunity (p < 0.05). The composite super energy extract M (CSEE-M), further optimized on the basis of SQ, significantly improved growth performance and feed conversion ratio, and elevated the activity of intestinal digestive enzymes (p < 0.05) in both mice and broilers and reshaped the gut microbiota of broilers. The addition of 0.5% CSEE-M to broiler drinking water significantly increased muscle content and improved carcass quality (p < 0.05). In conclusion, both SQ and CSEE-M hold great promise as NAGPs and serve as effective substitutes to AGPs. This research not only furnishes new solutions for the misuse of antibiotics but presents a fresh perspective for the development of growth promoters.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant Factor C as an In Vitro Assay for the Residual Pathogenicity Evaluation of Veterinary Autogenous Vaccines. 重组因子C在动物自体疫苗残留致病性评价中的应用

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-12-21 DOI: 10.3390/vetsci11120673

Antonella Di Paolo, Rosario Liberti, Lucia Anzalone, Claudia Colabella, Andrea Felici, Giulio Severi, Monica Cagiola

{"title":"Recombinant Factor C as an In Vitro Assay for the Residual Pathogenicity Evaluation of Veterinary Autogenous Vaccines.","authors":"Antonella Di Paolo, Rosario Liberti, Lucia Anzalone, Claudia Colabella, Andrea Felici, Giulio Severi, Monica Cagiola","doi":"10.3390/vetsci11120673","DOIUrl":"10.3390/vetsci11120673","url":null,"abstract":"Background: Veterinary autogenous vaccines, similar to all injectable pharmaceutical products, must be tested to assess endotoxin concentrations. The Limulus Amebocyte Lysate Test (LAL test) is widely used in in vitro quality control assays for endotoxin detection, although it presents some ethical issues related to the production of reagents and is also characterized by a low specificity due to other contaminants that can activate the reaction. For all these reasons, a new recombinant factor C LAL test was developed.Aim: In this study, we described the comparison between two LAL test methods for in vitro quality control of veterinary autogenous vaccines, with the aim of evaluating the most suitable method and establishing an endotoxin concentration range for two different matrices.Methods: Two hundred batches of two different vaccine matrices were tested using the kinetic chromogenic LAL test and recombinant factor C endotoxin detection assay commercial kits.Results and conclusions: Statistical analysis conducted after the validation of the recombinant factor C test exhibited a statistically significant correlation between the two methods and for both vaccine matrices, suggesting that the animal-free assay can be used as a routine quality control test for veterinary autogenous vaccines.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial Effect of Canine Leucocyte Platelet-Rich Plasma (L-PRP) and Canine Platelet-Poor Plasma (PPP) Against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus pseudintermedius. 犬白细胞富血小板血浆（L-PRP）和犬血小板贫血浆（PPP）对甲氧西林敏感和耐甲氧西林假中间葡萄球菌的抗菌作用

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-12-20 DOI: 10.3390/vetsci11120670

Roberta Perego, Gabriele Meroni, Piera Anna Martino, Eva Spada, Luciana Baggiani, Daniela Proverbio

{"title":"Antibacterial Effect of Canine Leucocyte Platelet-Rich Plasma (L-PRP) and Canine Platelet-Poor Plasma (PPP) Against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus pseudintermedius.","authors":"Roberta Perego, Gabriele Meroni, Piera Anna Martino, Eva Spada, Luciana Baggiani, Daniela Proverbio","doi":"10.3390/vetsci11120670","DOIUrl":"10.3390/vetsci11120670","url":null,"abstract":"Staphylococcus pseudintermedius (SP) is a commensal and opportunistic pathogen of skin and mucosal surfaces, isolated from healthy dogs and from canine pyoderma cases. It has recently gained attention due to its increasing antibiotic resistance. Platelet-rich plasma (PRP) is a biological product, obtained through a blood centrifugation process, which has antibacterial properties evidenced by in vitro and in vivo studies conducted in both the human and veterinary field. This in vitro study evaluated the antimicrobial effect of canine non-activated and activated leucocyte-rich PRP (L-PRP) and platelet-poor plasma (PPP) against two strains of SP isolated from dogs with pyoderma: one a multidrug-resistant strain (MDR) and one a non-MDR strain. Twenty healthy un-sedated adult blood donor dogs were enrolled for L-PRP and PPP production via a closed semi-automatic system for veterinary use. The evaluation of antimicrobial effect was performed using the micro-inhibition in broth method, exposing SP strains to 10 L-PRP, 10 activated L-PRP and 10 PPP samples, respectively. Bacterial growth was evaluated using CFU count at three timepoints (immediately after incubation T0, after 1 h T1 and after 2 h T2). L-PRP and PPP had a significant antimicrobial effect at all three timepoints which was similar against both non-MDR and MDR SP strains. Activation appeared to reduce the duration of the antimicrobial effect in L-PRP. More studies are necessary to confirm these preliminary results.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0