Veterinary Sciences最新文献

{"title":"Porcine Sample Type Characteristics Associated with Sequencing and Isolation of Influenza A Virus.","authors":"Daniel C A Moraes, Onyekachukwu H Osemeke, Michael A Zeller, Amy L Baker, Gustavo S Silva, Giovani Trevisan, Daniel C L Linhares, Phillip C Gauger","doi":"10.3390/vetsci12070683","DOIUrl":"10.3390/vetsci12070683","url":null,"abstract":"<p><p>Understanding how sample type may influence the probability of influenza A virus (IAV) sequencing and isolation success can help improve the use of diagnostic tests and refine surveillance strategies in swine populations. The objective of this study was to evaluate the probability of success for IAV hemagglutinin (HA) and neuraminidase (NA) Sanger sequencing and virus isolation in Madin-Darby Canine Kidney (MDCK) cells across different porcine sample types submitted to the Iowa State University Veterinary Diagnostic Laboratory (ISU VDL) from 2018 to 2024. Antemortem and postmortem sample types were selected and analyzed based on reverse transcription real-time polymerase chain reaction (RT-rtPCR) cycle threshold (Ct) values. The Ct values corresponding to 95%, 75%, and 50% probabilities of sequencing or virus isolation success were determined for each sample type. For antemortem samples, a 95% probability of success for HA Sanger sequencing on nasal swabs exhibited a Ct value of 27.8 from 1046 samples and 23.6 for NA sequencing based on 66 nasal swabs. Using oral fluids, HA and NA Sanger sequencing success was at Ct values of 27.3 from 3446 samples and 22.1 from 137 samples, respectively. For postmortem samples, lung tissue had the highest number of sequences for the HA and NA, with Ct values of 25.7 and 21.5, respectively. For a 95% probability of successful virus isolation, nasal swabs demonstrated a Ct value of 21.1 from 647 samples, while lungs had a Ct value of 18.7 from 5892 samples. This study determined that nasal swabs and lung tissue had the highest probability of IAV gene sequencing and virus isolation success, while oral fluids, a common swine diagnostic sample type that is easy to collect and welfare-friendly, can be effective for gene sequencing when using lower IAV RT-rtPCR Ct values, i.e., ≤27.3. These results provide practical expectations for successful IAV HA and NA gene sequencing and virus isolation at 95%, 75%, and 50% probabilities based on sample type and RT-rtPCR Ct values to improve diagnostic testing strategies in swine populations.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12298594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Equine Colostrum-Derived Mesenchymal Stromal Cells: A Potential Resource for Veterinary Regenerative Medicine.","authors":"Angelita Capone, Barbara Merlo, Fabiana Begni, Eleonora Iacono","doi":"10.3390/vetsci12070681","DOIUrl":"10.3390/vetsci12070681","url":null,"abstract":"<p><p>Beyond its immunological role, colostrum has emerged as a promising, non-invasive source of bioactive factors, including mesenchymal stem/stromal cells (MSCs). This study represents the first attempt to isolate and characterize MSCs from equine colostrum (C-MSCs) to assess their potential use in veterinary regenerative medicine. Colostrum (<i>n</i> = 6) was collected from mares immediately after their delivery and centrifuged, and the recovered cells were cultured under standard conditions. The C-MSCs displayed plastic adherence and a heterogeneous morphology, including spindle-shaped and epithelial-like cells. The population doubling time (PDT) values varied among the samples, and four out of six showed rapid proliferation (<2 days). Colony-forming unit (CFU) assays confirmed their clonogenic potential, though significant inter-sample variability was observed (<i>p</i> < 0.05). Spheroid formation assays revealed differences in cell-cell adhesion: four out of six samples formed stable spheroids within four days. A migration assay showed significant variability (<i>p</i> < 0.05): one out of six achieved complete wound closure within 72 h, whereas five out of six reached ~30% at 96 h. All samples were positive for adipogenic, chondrogenic, and osteogenic differentiation as shown via staining. RT-PCR confirmed MSC marker expression, while hematopoietic markers were absent. MHC-I expression was weak in five out of six samples, whereas MHC-II was consistently negative. These findings support equine colostrum as a viable MSC source, though its variability requires further validation with larger samples. Additional research is needed to investigate C-MSCs' immunomodulatory properties and therapeutic potential.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12300667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finite Element Analysis of Stress Distribution in Canine Lumbar Fractures with Different Pedicle Screw Insertion Angles.","authors":"Ziyao Zhou, Xiaogang Shi, Jiahui Peng, Xiaoxiao Zhou, Liuqing Yang, Zhijun Zhong, Haifeng Liu, Guangneng Peng, Chengli Zheng, Ming Zhang","doi":"10.3390/vetsci12070682","DOIUrl":"10.3390/vetsci12070682","url":null,"abstract":"<p><p>Pedicle screw fixation is a critical technique for stabilizing lumbar fractures in canines, yet the biomechanical implications of insertion angles remain underexplored. This study aims to identify optimal screw trajectories by analyzing stress distribution and deformation patterns in beagle lumbar segments (L6-L7) using finite element analysis (FEA). A 3D finite element model was reconstructed from CT scans of a healthy beagle, incorporating cortical/cancellous bone, intervertebral disks, and cartilage. Pedicle screws (2.4 mm diameter, 22 mm length) were virtually implanted at angles ranging from 45° to 65°. A 10 N vertical load simulated standing conditions. Equivalent stress and total deformation were evaluated under static loading. The equivalent stress occurred at screw-rod junctions, with maxima at 50° (11.73 MPa) and minima at 58° (3.25 MPa). Total deformation ranged from 0.0033 to 0.0064 mm, with the highest at 55° and the lowest at 54°. The 58° insertion angle demonstrated optimal biomechanical stability with minimal stress concentration, with 56-60° as a biomechanically favorable range for pedicle screw fixation in canine lumbar fractures, balancing stress distribution and deformation control. Future studies should validate these findings in multi-level models and clinical settings.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12301062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validating Sperm Concentration in Rabbit Cryopreservation Protocol: Implications for Fertility, Litter Size, and Offspring Growth.","authors":"Michele Di Iorio, Giusy Rusco, Fabrizio Lauriola, Emanuele Antenucci, Alessandra Roncarati, Silvia Cerolini, Michele Schiavitto, Nicolaia Iaffaldano","doi":"10.3390/vetsci12070678","DOIUrl":"10.3390/vetsci12070678","url":null,"abstract":"<p><p>The cryopreservation of rabbit semen is a valuable strategy for genetic resource preservation and efficient artificial insemination, but outcomes remain inconsistent, partly due to variations in sperm concentration per dose. This study aimed to evaluate the in vivo effects of different sperm concentrations (15, 25, 35, 55, and 75 million per straw) on fertility, prolificacy, and offspring growth in nulliparous and multiparous does. A total of 384 rabbit females were inseminated using frozen-thawed semen, and their reproductive performance was compared with fresh semen. Fertility and kindling rates varied with sperm concentration and parity: nulliparous does showed the highest fertility at 15 million sperm/straw (84.4%), while multiparous does reached peak values at 25-55 million/straw (78.1-81.3%). Litter size and live-born kits were consistently higher in multiparous than in nulliparous does. Offspring body weight at 19 and 60 days was influenced by both sperm concentration and maternal parity, with better growth generally observed in multiparous groups. Weaning success remained high across all groups. Our results indicate that sperm concentrations ranging from 15 to 35 × 10⁶/straw are the most suitable for cryopreservation, as they maintain high fertility, prolificacy, and offspring growth, comparable to fresh semen. These results confirm that optimizing sperm concentration during cryopreservation improves reproductive efficiency and that tailoring insemination strategies to the physiological status of the female enhances outcomes. The results provide useful recommendations for improving cryopreservation techniques in rabbit breeding programs.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12299526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Dietary Supplementation of Biochars on Growth Performance, Bone Mineralization, Footpad Health, Lymphoid Organs Weight, Antibody Titers of Newcastle Disease and Infectious Bronchitis Disease in Broiler Chicks.","authors":"Raheel Pervaiz, Riaz Mustafa, Umar Farooq, Waseem Abbas, Muhammad Farooq Khalid, Abdur Rehman, Munawar Hussain, Muhammad Muzammil Riaz, Asfa Fatima, Muhammad Aziz Ur Rahman","doi":"10.3390/vetsci12070680","DOIUrl":"10.3390/vetsci12070680","url":null,"abstract":"<p><p>This study evaluated the effect of biochars on growth performance, nutrient digestibility, carcass yield, bone mineralization, litter quality and footpad lesions in broilers. Eight hundred day-old chicks were randomly divided into four treatments, 10 replicates per treatment (20 birds/replicate) for 35 days. Treatments were basal diet (control), a control diet with corncob (CC) biochar (1%), a control diet with wheat straw (WS) biochar (1%) and a control diet with sugarcane bagasse (SCB) biochar (1%). Body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR) were recorded weekly. Nutrient digestibility, bone mineralization and carcass parameters were determined on the 21st and 35th days, while footpad lesions and litter quality were also assessed. The results revealed significant improvement (<i>p</i> < 0.05) in FI, BWG and FCR with supplementation. Nutrient digestibility was higher (<i>p</i> < 0.05) in the SCB biochar group. Tibia calcium and phosphorus levels were enhanced (<i>p</i> < 0.05) in the WS and SCB biochar groups, respectively. Footpad lesions were significantly lower (<i>p</i> < 0.05) in the CC biochar group, while litter quality was improved (<i>p</i> < 0.05) in the WS biochar group. Lymphoid organ relative weight results revealed that spleen weight was not affected by biochar supplementation in diet (<i>p</i> > 0.05), while dietary supplementation of CS and WS biochar in the diet resulted in the highest relative weights of thymus and bursa (<i>p</i> < 0.05). However, dietary supplementation of WS, SC and SCB biochar supplementation had affected positively the log value of the ND virus and IBV titers in birds. Overall, dietary supplementation of 1% biochars enhances growth performance, bone mineralization, footpad health immunity and litter quality in broilers.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12298835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Molecular Characterization of Swine Parainfluenza Virus 5 from Piglets Co-Infected with PEDV.","authors":"Yuling Ma, Xinxin Chen, Mengyao Ma, Xiaolong Gao, Ruoqi Song, Yue Yi, Ying Wang, Sheng Niu, Yujun Zhao, Wenxia Tian, Jianle Ren, Fang Yan","doi":"10.3390/vetsci12070676","DOIUrl":"10.3390/vetsci12070676","url":null,"abstract":"<p><p>Parainfluenza virus 5 (PIV5), now reclassified as <i>Orthorubulavirus mammalis</i>, is a negative-sense, single-stranded RNA virus belonging to the genus <i>Orthorubulavirus</i> of the family <i>Paramyxoviridae</i> [...].</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12299374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field-Based Characterization of Peste des Petits Ruminants in Sheep in Romania: Clinical, Pathological, and Diagnostic Perspectives.","authors":"Romică Iacobescu-Marițescu, Adriana Morar, Viorel Herman, Emil Tîrziu, János Dégi, Kálmán Imre","doi":"10.3390/vetsci12070679","DOIUrl":"10.3390/vetsci12070679","url":null,"abstract":"<p><p>Peste des petits ruminants is a highly contagious transboundary viral disease that poses a serious threat to small ruminant populations worldwide. In 2024, seven outbreaks of PPR were recorded in sheep flocks from Timiș County, marking the second confirmed incursions of peste des petits ruminants virus (PPRV) in Romania. This study aimed to document the clinical presentation, pathological findings, and diagnostic confirmation with these field outbreaks. Comprehensive field investigations were carried out between July and September 2024, including clinical examinations, post mortem analysis, serological screening, and molecular detection using reverse transcription polymerase chain reaction (RT-PCR). A total of 13,203 sheep were evaluated, with an overall mortality rate of 12.77%. Characteristic clinical signs included mucopurulent nasal discharge, oral erosions, respiratory distress, and diarrhea. Gross lesions observed during necropsy included hemorrhagic bronchopneumonia, bile-stained liver, catarrhal enteritis, and mucosal hemorrhages. Serological testing revealed flock-level seroprevalence rates ranging from 46.7% to 80.0%, with higher rates observed in older animals. RT-PCR confirmed PPRV infection in all affected flocks. Our findings provide strong evidence of virulent PPRV circulation in an area where the virus had not been reported before. The results highlight an urgent need to strengthen surveillance systems, enhance diagnostic capacity, and foster cross-border collaboration. These field-based insights can contribute to both national and international efforts aimed at controlling and ultimately eradicating the disease.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12298021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chiari-like Malformation and Syringomyelia in Pomeranians: A Longitudinal Study.","authors":"Mees R Jansma, Marieke van den Heuvel, Kenny Bossens, Erik Noorman, Michelle Hermans, Paul J J Mandigers","doi":"10.3390/vetsci12070677","DOIUrl":"10.3390/vetsci12070677","url":null,"abstract":"<p><strong>Background: </strong>Chiari-like malformation (CM) and syringomyelia (SM) are commonly observed conditions in Pomeranian dogs. Affected dogs may develop clinical signs that significantly impact quality of life. Therefore, it is crucial to select only unaffected dogs for breeding. However, the progression of CM/SM has not been fully elucidated. Dogs that are unaffected or mildly affected may progress to severe SM over time. The primary aim of this study is to investigate the progression of CM/SM through repeated MRI scans. A secondary objective is to evaluate the effect of furosemide treatment on syrinx sizes, given its frequent prescription.</p><p><strong>Methods: </strong>Pomeranians that underwent two CM/SM screenings between 2015 and 2025 were included. CM/SM classifications were assessed, and quantitative syrinx measurements were conducted. Maximum syrinx diameter (MSD) and maximum syrinx-to-spinal cord diameter ratio (MSD/SCD-r) were measured and documented. Dogs were classified based on the progression of SM. Furosemide treatment was documented, and its effect on syrinx size was compared with that in dogs not receiving furosemide.</p><p><strong>Results: </strong>At the time of the second MRI, 39.6% of dogs either developed SM or showed substantial progression, whereas 12.5% demonstrated partial recovery. Of the dogs initially classified as free from SM, 20.7% had developed the condition. A significant increase was observed in both MSD (<i>p</i> = 0.0058) and MSD/SCD-r (<i>p</i> = 0.0038) between MRI1 and MRI2. Notably, the change in MSD between MRI1 and MRI2 was statistically significantly smaller in dogs treated with furosemide compared to untreated dogs (<i>p</i> = 0.030).</p><p><strong>Conclusions: </strong>These findings indicate that syrinx dimensions are dynamic and may fluctuate over time, although a general trend toward progression is observed. Furthermore, furosemide may mitigate the progression of SM.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12299236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Comes from Cytology Diagnosis: A Comprehensive Epidemiological Retrospective Analysis of 3068 Feline Cases.","authors":"Paula Brilhante-Simões, Ricardo Lopes, Leonor Delgado, Ana Machado, Augusto Silva, Ângela Martins, Ricardo Marcos, Felisbina Queiroga, Justina Prada","doi":"10.3390/vetsci12070671","DOIUrl":"10.3390/vetsci12070671","url":null,"abstract":"<p><p>This study evaluated diagnostic trends and the overall utility of cytology in feline patients through the analysis of a large, multicentric dataset from Portugal. A retrospective review of 3068 cytological cases from 130 veterinary practices was conducted, with samples categorised by anatomical location and lesion type. Diagnostic outcomes were statistically assessed, revealing an overall success rate of 66.20%. The highest diagnostic yields occurred in fluid samples (83.48%), glandular tissues (76.67%), and mucous membranes (75.81%), followed by organ-based samples (67.79%), miscellaneous tissues (66.98%), cutaneous/subcutaneous nodules (62.16%), and lymph nodes (57.93%). Neoplastic lesions showed age-associated prevalence, being more common in older cats, with epithelial and melanocytic lesions more frequent in females and round cell/mesenchymal lesions predominating in males. Non-diagnostic samples (33.80%) primarily resulted from insufficient cellularity or suboptimal quality, though no significant correlation existed between diagnostic success and clinical setting. This study underscores that cytology remains a fundamental diagnostic tool in feline medicine, particularly when combined with proper sampling techniques and complementary diagnostic methods, and reinforces its value in clinical decision-making, thereby supporting its broader utilisation in routine veterinary practice.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12298540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Analysis of Antibiotic Resistance and Virulence Profiles in <i>Escherichia coli</i> Linked to Sternal Bursitis in Chickens: A One Health Perspective.","authors":"Jessica Ribeiro, Vanessa Silva, Catarina Freitas, Pedro Pinto, Madalena Vieira-Pinto, Rita Batista, Alexandra Nunes, João Paulo Gomes, José Eduardo Pereira, Gilberto Igrejas, Lillian Barros, Sandrina A Heleno, Filipa S Reis, Patrícia Poeta","doi":"10.3390/vetsci12070675","DOIUrl":"10.3390/vetsci12070675","url":null,"abstract":"<p><p>Sternal bursitis is an underexplored lesion in poultry, often overlooked in microbiological diagnostics. In this study, we characterized 36 <i>Escherichia coli</i> isolates recovered from sternal bursitis in broiler chickens, combining phenotypic antimicrobial susceptibility testing, PCR-based screening, and whole genome sequencing (WGS). The genetic analysis revealed a diverse population spanning 15 sequence types, including ST155, ST201, and ST58. Resistance to tetracycline and ciprofloxacin was common, and several isolates carried genes encoding β-lactamases, including <i>blaTEM-1B</i>. Chromosomal mutations associated with quinolone and fosfomycin resistance (e.g., <i>gyrA p.S83L</i>, <i>glpT_E448K</i>) were also identified. WGS revealed a high number of virulence-associated genes per isolate (58-96), notably those linked to adhesion (<i>fim</i>, <i>ecp</i> clusters), secretion systems (T6SS), and iron acquisition (<i>ent</i>, <i>fep</i>, <i>fes</i>), suggesting strong pathogenic potential. Many isolates harbored virulence markers typical of ExPEC/APEC, such as <i>iss</i>, <i>ompT</i>, and <i>traT</i>, even in the absence of multidrug resistance. Our findings suggest that <i>E. coli</i> from sternal bursitis may act as reservoirs of resistance and virulence traits relevant to animal and public health. This highlights the need for including such lesions in genomic surveillance programs and reinforces the importance of integrated One Health approaches.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12298035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}