Veterinary Sciences最新文献

{"title":"Analytical Validation of Two Point-of-Care Assays for Hematological Analysis in the Miranda Donkey.","authors":"Céline Costa, Ana Patrícia Sousa, Grasiene Silva, Felisbina Queiroga, Ângela Martins, Daniela Andrade, Ana C Silvestre-Ferreira","doi":"10.3390/vetsci11090450","DOIUrl":"https://doi.org/10.3390/vetsci11090450","url":null,"abstract":"<p><p>Miranda donkeys are an endangered autochthonous breed of Portugal. The HemoCue WBC DIFF and HemoCue Hb 201 portable analyzers, developed as a simplified alternative method for total and differential WBC count and hemoglobin measurement, respectively, may be relevant tools in veterinary practice. This study aimed to validate these instruments using Miranda donkey blood samples. For the HemoCue WBC DIFF, most parameters showed acceptable intra- and inter-assay precision with coefficients of variation (CV) below 5%, except for monocytes and eosinophils with higher CVs. The HemoCue Hb 201 showed CVs of 1.98% and 4.07%. Linearity correlation coefficients (r) ranged from 0.53 to 0.99 for HemoCue WBC DIFF and 0.99 for HemoCue Hb 201. Significant levels for neutrophils, lymphocytes, monocytes, eosinophils, and Hb measurements varied. Comparisons with ProCyte Dx showed an excellent correlation for WBC (r_s = 0.96), neutrophils (r_s = 0.91), lymphocytes (r_s = 0.94), and eosinophils (r_s = 0.90) but a poor correlation for monocytes and basophils. The HemoCue Hb 201 showed a correlation of r_s = 0.96 with ProCyte Dx. In conclusion, both analyzers provided reliable results and are suitable for use in Miranda's donkey breed for WBC counts and Hb measurements.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11435812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dog Blood Type DEA 1 in Two Municipalities of Luanda Province of Angola (Sub-Saharan Africa).","authors":"Ana C Silvestre-Ferreira, Hugo Vilhena, Ana C Oliveira, José R Mendoza, Maria Garcia Aura, Josep Pastor","doi":"10.3390/vetsci11090449","DOIUrl":"https://doi.org/10.3390/vetsci11090449","url":null,"abstract":"<p><p>In dogs, the risk of an acute hemolytic transfusion reaction at the first transfusion is negligible; however, mismatched transfusions may produce alloimmunization. To avoid fatal acute hemolytic reactions in subsequent blood transfusions, it is important to recognize blood groups and to blood type both the donor and the recipient. Prevalence of dog blood groups varies geographically and between breeds. Our aim was to determine DEA 1 prevalence in a canine population in Luanda (Angola) and to assess alloimmunization risk after a mismatched blood transfusion. Blood samples were typed using an immunochromatographic strip technique. Of the 112 dogs tested (59 males; 53 females), 52.68% were DEA 1 positive and 47.32% DEA 1 negative. Females tended to be DEA 1 positive, and males DEA 1 negative (<i>p</i> = 0.0085). In a first-time mismatched blood transfusion, the calculated probability of a dog becoming sensitized was 24.9% and the probability of an acute hemolytic reaction following a second incompatible blood transfusion was 6.21%. DEA 1 prevalence obtained was similar to that reported worldwide, but differs from other African countries. The risk of alloimmunization and acute hemolytic transfusion reactions in mismatched blood transfusions is higher than that in other African regions. Blood typing is recommended prior to transfusion.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11436134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Multiplex PCR Assay for Simultaneous Detection of <i>Giardia duodenalis</i>, <i>Cryptosporidium parvum</i>, <i>Blastocystis</i> spp. and <i>Enterocytozoon bieneusi</i> in Goats.","authors":"Xingang Yu, Hui Xu, Xuanru Mu, Kaijian Yuan, Yilong Li, Nuo Xu, Qiaoyu Li, Wenjing Zeng, Shengfeng Chen, Yang Hong","doi":"10.3390/vetsci11090448","DOIUrl":"https://doi.org/10.3390/vetsci11090448","url":null,"abstract":"<p><p><i>Giardia duodenalis</i>, <i>Cryptosporidium parvum</i>, <i>Blastocystis</i> spp. and <i>Enterocytozoon bieneusi</i> are four common zoonotic parasites associated with severe diarrhea and enteric diseases. In this study, we developed a multiplex PCR assay for the simultaneous detection of these four zoonotic protozoans in goat stool samples and assessed its detection efficiency. Specific primers were designed from conserved gene sequences retrieved from GenBank, and the PCR conditions were optimized. Genomic DNA from 130 samples was subjected to both single-target PCR and multiplex PCR. The multiplex PCR assay successfully amplified specific gene fragments (<i>G. duodenalis</i>, 1400 bp; <i>C. parvum</i>, 755 bp; <i>Blastocystis</i> spp., 573 bp; <i>E. bieneusi</i>, 314 bp). The assay sensitivity was ≥10² copies of pathogenic DNA clones with high specificity confirmed by negative results for other intestinal parasites. The detection rates were 23.08% (30/130) for <i>G. duodenalis</i>, 24.62% (32/130) for <i>C. parvum</i>, 41.54% (54/130) for <i>Blastocystis</i> spp., and 12.31% (16/130) for <i>E. bieneusi</i>, matching the single-target PCR results. The sensitivity and predictive values were 100.00%. This multiplex PCR provided a rapid, sensitive, specific, and cost-effective approach for detecting these four parasites. It also provided essential technical support for the rapid detection and epidemiological investigation of <i>G. duodenalis</i>, <i>C. parvum</i>, <i>Blastocystis</i> spp., and <i>E. bieneusi</i> infections in goat fecal samples.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11435618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization Studies on the <i>sugC</i> Gene of <i>Streptococcus suis</i> Serotype 2 in Adhesion, Invasion, and Virulence in Mice.","authors":"Zhimin Dong, Cheng Li, Xiangxue Tian, Xiaoran Guo, Xiuli Li, Weike Ren, Jingjing Chi, Li Zhang, Fuqiang Li, Yao Zhu, Wanjiang Zhang, Minghua Yan","doi":"10.3390/vetsci11090447","DOIUrl":"https://doi.org/10.3390/vetsci11090447","url":null,"abstract":"<p><p>The <i>sugC</i> gene of <i>Streptococcus suis</i> (<i>S. suis</i>) is a coding gene for the ATP-binding transporter-associated protein with strong pathogenicity. In order to reveal the effect of the <i>sugC</i> gene on the virulence of <i>S. suis</i> serotype 2, a wild-type strain of TJS75, isolated from fattening pigs' brain tissue samples, was used as a parent strain, and a knockout <i>sugC</i> gene (Δ<i>sugC</i>) and complementary strain (CΔ<i>sugC</i>) were successfully constructed via homologous recombination technology. The biological characteristics of TJS75, Δ<i>sugC</i> and CΔ<i>sugC</i> were compared and analyzed through growth curves, biochemical characteristics, hemolysis characteristics, cell infection tests and pathogenicity tests on BALB/c mice. The results of the growth characteristic experiments in vitro showed that the plateau stage growth period of Δ<i>sugC</i> was delayed compared to the TJS75 strain, but there was no difference in the total number of bacteria. The biochemical characteristics and hemolysis ability of Δ<i>sugC</i> in sheep blood had no difference compared with TJS75, but its adhesion and invasion abilities in PK-15 cells were decreased. Knockout of the <i>sugC</i> gene had no impact on the expression levels of adhesion-related genes in TJS75 in real-time PCR analysis. In addition, the LD₅₀ of Δ<i>sugC</i> in BALB/c mice was 1.47 × 10⁸ CFU, seven times higher than that of TJS75 (LD₅₀ = 2.15 × 10⁷ CFU). These results illustrate that the deletion of <i>sugC</i> reduced the virulence of TJS75 to BALB/c mice, but its role in the adhesion and invasion of PK-15 cells in this strain needs to be further explored. In summary, this study provides evidence that the <i>sugC</i> gene is a virulence-related gene in the <i>S. suis</i> serotype 2 strain and plays a crucial role in the adhesion and invasion of <i>S. suis</i>. This study lays a foundation for the further exploration of the potential virulence factors and pathogenesis of <i>S. suis</i>.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11435659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saliva as a Potential Source of Biomarkers in Cows with Metritis: A Pilot Study.","authors":"Pedro J Vallejo-Mateo, María D Contreras-Aguilar, Alberto Muñoz-Prieto, María Botia, Asta Tvarijonaviciute, Camila Peres Rubio, Rasa Zelvyte, José J Cerón, Lorena Franco-Martínez","doi":"10.3390/vetsci11090446","DOIUrl":"https://doi.org/10.3390/vetsci11090446","url":null,"abstract":"<p><p>Metritis affects 5-20% of cows after parturition, negatively impacting animal welfare and the profitability of dairy farms, increasing culling rates and costs, and decreasing productivity and reproduction rates. This study compared the results of a comprehensive biochemical panel consisting of 25 salivary and 31 serum analytes between healthy cows (<i>n</i> = 16) and cows with metritis (<i>n</i> = 12). Descriptive parameters such as depression, rectal temperature, body condition score (BCS), heart rate, respiratory rate, mucous color, ruminal motility, vaginal discharge, milk production, and complete hematology analyses were also assessed for comparative purposes. The biochemistry analytes comprised five analytes related to stress, five to inflammation, five to oxidative status, and nineteen to general metabolism. The two-way ANOVA analysis revealed that, in saliva, eight biomarkers (lipase, adenosine deaminase (ADA), haptoglobin (Hp), total proteins, g-glutamyl transferase (gGT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and creatine kinase (CK)) were significant higher in cows with metritis. In serum, eight biomarkers (ADA, Hp, serum amyloid A (SAA), fibrinogen, ferritin, AOPPs/albumin ratio, non-esterified fatty acids (NEFAs), and bilirubin) were significantly higher in cows with metritis, whereas six (total esterase (TEA), albumin, urea, lactate, phosphorus, and calcium) were lower. Of the total number of 23 biomarkers that were measured in both saliva and serum, significant positive correlations between the two biofluids were found for six of them (Hp, FRAP, CUPRAC, AOPPs, urea, and phosphorus). Urea showed an R = 0.7, and the correlations of the other analytes were weak (R < 0.4). In conclusion, cows with metritis exhibited differences in biomarkers of stress, inflammation, cellular immune system, and general metabolism in both salivary and serum biochemistry profiles. These changes were of different magnitudes in the two biofluids. In addition, with the exception of ADA and Hp, the analytes that showed changes in the saliva and serum profiles of cows affected by metritis were different. Overall, this report opens a new window for the use of saliva as potential source of biomarkers in cows with metritis.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11435728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Relationship between proAKAP4 Level and Longevity of Sexed Sperm Quality after Thawing.","authors":"İlktan Bastan, Fırat Korkmaz, Derya Şahin, Seher Şimşek, Ufuk Kaya","doi":"10.3390/vetsci11090444","DOIUrl":"https://doi.org/10.3390/vetsci11090444","url":null,"abstract":"<p><p>ProAKAP4 is a sperm structural protein that regulates motility through the PKA-dependent cAMP signaling pathway, which is synthesized as an X chromosome-linked member of the gene family. This study aims to determine the optimal level of proAKAP4 for evaluating sexed semen through investigating its relationship with the longevity of sperm quality in sexed Holstein bull sperm. A total of 30 sexed sperm samples (bearing X chromosomes) from 30 distinct Holstein bulls (<i>n</i> = 30) were analyzed. The frozen bull sperm samples were assessed for their proAKAP4 levels, mitochondrial membrane potential, plasma membrane and acrosome integrity (PMAI), and spermatozoa movement parameters at hours 0 and 3 after thawing. The proAKAP4 levels in the sexed sperm samples ranged from 16.35 to 72.10 ng/10 M spz, with an average of 37.18 ± 15.1 ng/10 M spz. A strong positive correlation was observed between proAKAP4 levels and total motility, progressive motility, PMAI, high mitochondrial membrane potential, VAP, and VCL values after 3 h of incubation, when compared to post-thaw analyses. The results also reveal that spermatozoa with proAKAP4 levels of ≥40 ng/10 M spz exhibit higher quality. In conclusion, the level of proAKAP4 in sexed sperm aligns with previous studies and shows potential as a biomarker for assessing the longevity of sexed sperm quality.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11436178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Analysis of the Hepatopancreas in the Sex-Related Size Differences of <i>Macrobrachium nipponense</i>.","authors":"Yabing Wang, Guangde Qiao, Yanfeng Yue, Shiming Peng, Hongtuo Fu","doi":"10.3390/vetsci11090445","DOIUrl":"https://doi.org/10.3390/vetsci11090445","url":null,"abstract":"<p><p><i>Macrobrachium nipponense</i>, a commercially popular crustacean species within the Chinese context, is recognized for its exceptional nutritional composition and palatability. There are significant differences in growth between male and female <i>M. nipponense</i>. Herein, transcriptomics was used to determine the hepatopancreas transcriptome differences between sex-related size differences in <i>M. nipponense</i>. We identified 974 differentially expressed genes (DEGs) between the SHE (female) and BHE (male) groups, which were validated using RT-qPCR. The genes encoding matrix metalloproteinase-9 (<i>MM9</i>), Ribosome-binding protein 1 (<i>RBP1</i>), Aly/REF export factor 2, and hematological and neurological expressed 1 (<i>HN1</i>) may play a role in modulating the sex-related size differences observed in <i>M. nipponense</i>. Clusters of orthologous groups and gene ontology functional analysis demonstrated that the DEGs for sex-related size in <i>M.nipponense</i> were associated with various biological functions. The Kyoto Encyclopedia of Genes and Genomes pathways analysis demonstrated that upregulated DEGs were mainly enriched in lysine biosynthesis, tryptophan metabolism, and lysine degradation pathways, whereas the downregulated DEGs were mainly enriched in ascorbate and aldarate metabolism, retinol metabolism, and drug metabolism-cytochrome P450 pathways. The results indicated the molecular mechanism underlying the sex-related size differences and identified key genes. This data will be invaluable to support explanations of individual differences between male and female prawns.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11435631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Diagnostic Value of qPCR Quantification of <i>Paenibacillus larvae</i> in Hive Debris and Adult Bees for Predicting the Onset of American Foulbrood.","authors":"Bojan Papić, Lucija Žvokelj, Metka Pislak Ocepek, Barbara Hočevar, Monika Kozar, Rene Rus, Urška Zajc, Darja Kušar","doi":"10.3390/vetsci11090442","DOIUrl":"https://doi.org/10.3390/vetsci11090442","url":null,"abstract":"<p><p>American foulbrood (AFB) is a serious infectious disease of honeybees (<i>Apis mellifera</i>) caused by <i>Paenibacillus larvae</i>. Increased <i>P. larvae</i> count in hive-related material is associated with an increased risk of AFB. Here, we quantified <i>P. larvae</i> cells in 106 adult bee and 97 hive debris samples using quantitative PCR (qPCR); 66/106 adult bee and 66/97 hive debris samples were collected simultaneously from the same bee colony (paired-sample design). The corresponding bee colonies were also examined for the presence of AFB clinical signs. A binary logistic regression model to distinguish between AFB-affected and unaffected honeybee colonies showed a strong diagnostic accuracy of both sample types for predicting the onset of AFB based on <i>P. larvae</i> counts determined by qPCR. The colonies with a <i>P. larvae</i> count greater than 4.5 log cells/adult bee or 7.3 log cells/mL hive debris had a 50% probability of being clinically affected and were categorized as high-risk. The AFB-unaffected colonies had significantly lower <i>P. larvae</i> counts than the AFB-affected colonies, but the latter did not differ significantly in <i>P. larvae</i> counts in relation to the severity of clinical signs. Both bee-related sample types had a high diagnostic value for predicting disease outcome based on <i>P. larvae</i> counts. These results improve the understanding of the relationship between <i>P. larvae</i> counts and AFB occurrence, which is essential for early detection of high-risk colonies.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11436083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142336661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraluminal Contrast-Enhanced Ultrasonography Application in Dogs and Cats.","authors":"Saran Chhoey, Soyeon Kim, Eunjee Kim, Dongjae Lee, Kroesna Kang, Sath Keo, Jezie Alix Acorda, Junghee Yoon, Jihye Choi","doi":"10.3390/vetsci11090443","DOIUrl":"https://doi.org/10.3390/vetsci11090443","url":null,"abstract":"<p><p>Administering intraluminal fluid can improve the acoustic window for the visualization of the lumen and wall layers in the cavitary organs. Microbubbles in ultrasound contrast agents can also be used for intracavitary applications to enhance visualization of the lesion in human patients. However, there was no literature extending the clinical application of intraluminal contrast-enhanced ultrasonography (CEUS) to patients with naturally occurring diseases in veterinary medicine. This case series aims to describe the detailed application and diagnostic value of intraluminal CEUS in six clinical cases with naturally occurring gastrointestinal (GI) and urinary tract diseases.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11436020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive Neuromarker Patterns for Calcification Metaplasia in Early Tendon Healing.","authors":"Melisa Faydaver, Valeria Festinese, Oriana Di Giacinto, Mohammad El Khatib, Marcello Raspa, Ferdinando Scavizzi, Fabrizio Bonaventura, Valentina Mastrorilli, Paolo Berardinelli, Barbara Barboni, Valentina Russo","doi":"10.3390/vetsci11090441","DOIUrl":"https://doi.org/10.3390/vetsci11090441","url":null,"abstract":"<p><p>Unsuccessful tendon healing leads to fibrosis and occasionally calcification. In these metaplastic drifts, the mouse AT preclinical injury model represents a robust experimental setting for studying tendon calcifications. Previously, calcium deposits were found in about 30% of tendons after 28 days post-injury. Although a neuromediated healing process has previously been documented, the expression patterns of NF200, NGF, NPY, GAL, and CGRP in mouse AT and their roles in metaplastic calcific repair remain to be explored. This study included a spatiotemporal analysis of these neuromarkers during the inflammatory phase (7 days p.i.) and the proliferative/early-remodelling phase (28 days p.i.). While the inflammatory phase is characterised by NF200 and CGRP upregulation, in the 28 days p.i., the non-calcified tendons (<i>n</i> = 16/24) showed overall NGF, NPY, GAL, and CGRP upregulation (compared to 7 days post-injury) and a return of NF200 expression to values similar to pre-injury. Presenting a different picture, in calcified tendons (<i>n</i> = 8), NF200 persisted at high levels, while NGF and NPY significantly increased, resulting in a higher NPY/CGRP ratio. Therefore, high levels of NF200 and imbalance between vasoconstrictive (NPY) and vasodilatory (CGRP) neuromarkers may be indicative of calcification. Tendon cells contributed to the synthesis of neuromarkers, suggesting that their neuro-autocrine/paracrine role is exerted by coordinating growth factors, cytokines, and neuropeptides. These findings offer insights into the neurobiological mechanisms of early tendon healing and identify new neuromarker profiles predictive of tendon healing outcomes.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11435825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}