The Royal Society of Chemistry最新文献

The US Preventive Services Task Force in Legal Jeopardy. 美国预防服务工作组陷入法律困境。

IF 3.6 3区化学

Analyst Pub Date : 2024-11-19 DOI: 10.1001/jama.2024.17377

Christopher Robertson, Kirsten Bibbins-Domingo, Gregory Curfman

引用次数: 0

Optical tweezer-assisted cell pairing and fusion for somatic cell nuclear transfer within an open microchannel. 开放式微通道内体细胞核移植的光镊辅助细胞配对与融合。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-11-19 DOI: 10.1039/d4lc00561a

Yidi Zhang, Han Zhao, Zhenlin Chen, Zhen Liu, Hanjin Huang, Yun Qu, Yaowei Liu, Mingzhu Sun, Dong Sun, Xin Zhao

{"title":"Optical tweezer-assisted cell pairing and fusion for somatic cell nuclear transfer within an open microchannel.","authors":"Yidi Zhang, Han Zhao, Zhenlin Chen, Zhen Liu, Hanjin Huang, Yun Qu, Yaowei Liu, Mingzhu Sun, Dong Sun, Xin Zhao","doi":"10.1039/d4lc00561a","DOIUrl":"10.1039/d4lc00561a","url":null,"abstract":"Somatic cell nuclear transfer (SCNT), referred to as somatic cell cloning, is a pivotal biotechnological technique utilized across various applications. Although robotic SCNT is currently available, the subsequent oocyte electrical activation/reconstructed embryo electrofusion is still manually completed by skilled operators, presenting challenges in efficient manipulation due to the uncontrollable positioning of the reconstructed embryo. This study introduces a robotic SCNT-electrofusion system to enable high-precision batch SCNT cloning. The proposed system integrates optical tweezers and microfluidic technologies. An optical tweezer is employed to facilitate somatic cells in precisely reaching the fusion site, and a specific polydimethylsiloxane (PDMS) chip is designed to assist in positioning and pairing oocytes and somatic cells. Enhancement in the electric field distribution between two parallel electrodes by PDMS pillars significantly reduces the required external voltage for electrofusion/electrical activation. We employed porcine oocytes and porcine fetal fibroblasts for SCNT experiments. The experimental results show that 90.56% of oocytes successfully paired with somatic cells to form reconstructed embryos, 76.43% of the reconstructed embryos successfully fused, and 70.55% of these embryos underwent cleavage. It demonstrates that the present system achieves the robotic implementation of oocyte electrical activation/reconstructed embryo electrofusion. By leveraging the advantages of batch operations using microfluidics, it proposes an innovative robotic cloning procedure that scales embryo cloning.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":"5215-5224"},"PeriodicalIF":6.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deamidation analysis of therapeutic drugs using matrix-assisted laser desorption ionization mass spectrometry and a novel algorithm QuanDA. 利用基质辅助激光解吸电离质谱法和新型算法 QuanDA 对治疗药物进行脱酰胺分析。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-19 DOI: 10.1039/d4ay01595a

Han Zhang, Yinran Xiong, Xiaonan Shi, Lijia Zhu, Qiong Wu, Ting Wu, Yiping Du

引用次数: 0

Recent advances in peptide macrocyclization strategies. 多肽大环化策略的最新进展。

IF 40.4 1区化学

Chemical Society Reviews Pub Date : 2024-11-19 DOI: 10.1039/d3cs01066j

Pengyuan Fang, Wing-Ka Pang, Shouhu Xuan, Wai-Lun Chan, Ken Cham-Fai Leung

{"title":"Recent advances in peptide macrocyclization strategies.","authors":"Pengyuan Fang, Wing-Ka Pang, Shouhu Xuan, Wai-Lun Chan, Ken Cham-Fai Leung","doi":"10.1039/d3cs01066j","DOIUrl":"https://doi.org/10.1039/d3cs01066j","url":null,"abstract":"Recently, owing to their special spatial structures, peptide-based macrocycles have shown tremendous promise and aroused great interest in multidisciplinary research ranging from potent antibiotics against resistant strains to functional biomaterials with novel properties. Besides traditional monocyclic peptides, many fascinating polycyclic and remarkable higher-order cyclic, spherical and cylindric peptidic systems have come into the limelight owing to breakthroughs in various chemical (e.g., native chemical ligation and transition metal catalysis), biological (e.g., post-translational enzymatic modification and genetic code reprogramming), and supramolecular (e.g., mechanically interlocked, metal-directed folding and self-assembly via noncovalent interactions) macrocyclization strategies developed in recent decades. In this tutorial review, diverse state-of-the-art macrocyclization methodologies and techniques for peptides and peptidomimetics are surveyed and discussed, with insights into their practical advantages and intrinsic limitations. Finally, the synthetic-technical aspects, current unresolved challenges, and outlook of this field are discussed.","PeriodicalId":68,"journal":{"name":"Chemical Society Reviews","volume":" ","pages":""},"PeriodicalIF":40.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"Sweet MOFs": exploring the potential and restraints of integrating carbohydrates with metal-organic frameworks for biomedical applications. "甜味 MOFs"：探索将碳水化合物与金属有机框架整合用于生物医学应用的潜力和限制。

IF 8 2区材料科学

Nanoscale Horizons Pub Date : 2024-11-19 DOI: 10.1039/d4nh00525b

Alessio Zuliani, Victor Ramos, Alberto Escudero, Noureddine Khiar

{"title":"\"Sweet MOFs\": exploring the potential and restraints of integrating carbohydrates with metal-organic frameworks for biomedical applications.","authors":"Alessio Zuliani, Victor Ramos, Alberto Escudero, Noureddine Khiar","doi":"10.1039/d4nh00525b","DOIUrl":"https://doi.org/10.1039/d4nh00525b","url":null,"abstract":"The unique features of metal-organic frameworks (MOFs) such as biodegradability, reduced toxicity and high surface area offer the possibility of developing smart nanosystems for biomedical applications through the simultaneous functionalization of their structure with biologically relevant ligands and the loading of biologically active cargos, ranging from small drugs to large biomacromolecules, into their pores. Aiming to develop efficient, naturally inspired biocompatible systems, recent research has combined organic and materials chemistry to design innovative composites that exploit carbohydrate chemistry for the functionalization and structural modification of MOFs. Scientific investigation in the field has seen a significant rise in the past five years, and it is becoming crucial to acknowledge both the limits and benefits of this approach for future investigation. In this review, the latest research results merging carbohydrates and MOFs are discussed, with a particular emphasis on the advances in the field and the remaining challenges, including addressing sustainability and real-case applicability.","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-emission carbon dots/gold nanoclusters fluorescent probe for ratiometric and colorimetric detection of Pb²⁺ and Hg². 用于比率和比色法检测 Pb2+ 和 Hg2 的碳点/金纳米团簇双发射荧光探针。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-19 DOI: 10.1039/d4ay01638f

Hongxin Cai, Yixin Shao, Lingling Yan, Hang Yu, Qiang Hu, Yongqiang Wang, Xiude Tu

{"title":"A dual-emission carbon dots/gold nanoclusters fluorescent probe for ratiometric and colorimetric detection of Pb2+ and Hg2.","authors":"Hongxin Cai, Yixin Shao, Lingling Yan, Hang Yu, Qiang Hu, Yongqiang Wang, Xiude Tu","doi":"10.1039/d4ay01638f","DOIUrl":"https://doi.org/10.1039/d4ay01638f","url":null,"abstract":"A novel fluorescent probe, carbon dots/gold nanoclusters (CDs/AuNCs), was prepared for ratiometric and colorimetric detection of Pb2+ and Hg2+ simultaneously. The as-prepared probe exhibited two distinct emission peaks at 445 nm and 610 nm. During the detection process, Pb2+ could quench blue fluorescence due to the formation of the CDs-Pb2+ complex and does not affect orange fluorescence. Conversely, Hg2+ could quench the orange fluorescence greatly due to the Hg2+-Au+ metallophilic interaction and keep blue fluorescence unaffected. Accordingly, ratiometric fluorescence sensing approaches of Pb2+ and Hg2+ are achieved and dramatic color changes from pink to orange/blue can be observed under UV illumination upon the addition of Pb2+/Hg2+. Furthermore, good linear relationships between the fluorescence intensity ratio (I610/I445) and Pb2+/Hg2+ concentration were demonstrated in ranges of 0-130 nM for Pb2+ and 0 to 280 nM for Hg2+, with detection limits of 6.5 nM for Pb2+ and 3.7 nM for Hg2+, respectively. The probe showed excellent selectivity and the mutual interference problem of coexistence of Pb2+ and Hg2+ could be effectively solved by introducing masking agents. In addition, the application of CDs/AuNCs proved successful in detecting Pb2+ and Hg2+ in actual water samples, yielding satisfactory recovery rates. These results indicated that the probe provides a new approach for visualizing and semi-quantitatively detecting Pb2+ and Hg2+, making it highly promising for monitoring environmental water samples.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid low-level nitrate determination by UV spectroscopy in the presence of competing ions. 在存在竞争离子的情况下利用紫外光谱快速测定低浓度硝酸盐。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-19 DOI: 10.1039/d4ay01641f

S E Mackay, D S Eldridge, F Malherbe

{"title":"Rapid low-level nitrate determination by UV spectroscopy in the presence of competing ions.","authors":"S E Mackay, D S Eldridge, F Malherbe","doi":"10.1039/d4ay01641f","DOIUrl":"https://doi.org/10.1039/d4ay01641f","url":null,"abstract":"The presence of excessive nitrate in environmental and drinking water even at low levels can pose both environmental and health hazards. Because of this, various methods for its removal have been investigated. Essential to conducting such research is a method to reliably quantify nitrate in relevant matrices. However, current approaches have drawbacks related to cost, analysis time or health hazards. This study evaluates a UV spectroscopy-based method for nitrate determination, suitable for quantifying low nitrate concentrations in the presence of bicarbonate, sulfate, chloride and phosphate. The limits of detection and quantification were found to be 0.003 ppm N and 0.0077 ppm N respectively, lower than that observed in the ion chromatography method it was compared to. Additionally, a calibration curve created with 11 standards ranging from 0 to 2.5 ppm N demonstrated an exceptionally strong correlation, with an R2 value greater than 0.9999. This method is developed with a focus on accessibility and speed, minimising hazards and waste generation for reduced environmental footprint. The effects of contaminants introduced through the use of commercial laboratory consumables are also discussed.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic FeO_x/FeWO_x nanocomposite memristor for neuromorphic and reservoir computing. 用于神经形态和存储计算的动态 FeOx/FeWOx 纳米复合忆阻器。

IF 5.8 3区材料科学

Nanoscale Pub Date : 2024-11-19 DOI: 10.1039/d4nr03762f

Muhammad Ismail, Maria Rasheed, Yongjin Park, Jungwoo Lee, Chandreswar Mahata, Sungjun Kim

{"title":"Dynamic FeOx/FeWOx nanocomposite memristor for neuromorphic and reservoir computing.","authors":"Muhammad Ismail, Maria Rasheed, Yongjin Park, Jungwoo Lee, Chandreswar Mahata, Sungjun Kim","doi":"10.1039/d4nr03762f","DOIUrl":"https://doi.org/10.1039/d4nr03762f","url":null,"abstract":"Memristors are crucial in computing due to their potential for miniaturization, energy efficiency, and rapid switching, making them particularly suited for advanced applications such as neuromorphic computing and in-memory operations. However, these tasks often require different operational modes-volatile or nonvolatile. This study introduces a forming-free Ag/FeOx/FeWOx/Pt nanocomposite memristor capable of both operational modes, achieved through compliance current (CC) adjustment and structural engineering. Volatile switching occurs at low CC levels (<500 μA), transitioning to nonvolatile at higher levels (mA). Operating at extremely low voltages (<0.2 V), this memristor exhibits excellent uniformity, data retention, and multilevel switching, making it highly suitable for high-density data storage. The memristor successfully mimics fundamental biological synapse functions, exhibiting potentiation, depression, and spike-rate dependent plasticity (SRDP). It effectively emulates transitions from short-term memory (STM) to long-term memory (LTM) by varying pulse characteristics. Leveraging its volatile switching and STM features, the memristor proves ideal for reservoir computing (RC), where it can emulate dynamic reservoirs for sequence data classification. A physical RC system, implemented using digits 0 to 9, achieved a recognition rate of 93.4% in off-chip training with a deep neural network (DNN), confirming the memristor's effectiveness. Overall, the dual-mode switching capability of the Ag/FeOx/FeWOx/Pt memristor enhances its potential for AI applications, particularly in temporal and sequential data processing.","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The study of the photochemical behavior of 5-aryl-2,3-dihydropyrazine 1,4-dioxides. 5- 芳基-2,3-二氢吡嗪-1,4-二氧化物的光化学行为研究。

IF 2.9 3区化学

Organic & Biomolecular Chemistry Pub Date : 2024-11-19 DOI: 10.1039/d4ob01570c

Nadezhda A Bakuleva, Boris V Lichitskii, Andrey N Komogortsev, Evgeny V Tretyakov

引用次数: 0

Enzymatic treatment to decrease the allergenicity of Pru p 3 from peach. 酶处理降低桃子中 Pru p 3 的过敏性。

IF 5.1 1区农林科学

Food & Function Pub Date : 2024-11-19 DOI: 10.1039/d4fo03052d

Ana P Tobajas, Ana Agulló-García, José L Cubero, Carlos Colás, Alba Civera, Clara Esteban, Lourdes Sánchez, María D Pérez

{"title":"Enzymatic treatment to decrease the allergenicity of Pru p 3 from peach.","authors":"Ana P Tobajas, Ana Agulló-García, José L Cubero, Carlos Colás, Alba Civera, Clara Esteban, Lourdes Sánchez, María D Pérez","doi":"10.1039/d4fo03052d","DOIUrl":"https://doi.org/10.1039/d4fo03052d","url":null,"abstract":"Pru p 3, a member of the lipid transfer protein family, is considered a major allergen from peach as it often induces serious allergic reactions in peach-allergic individuals. The high resistance of Pru p 3 to processing treatments and to digestion or enzymatic hydrolysis is probably the cause of the severity of this fruit allergy. The aim of this study was to determine the effect of treatment with a large number of proteases from different origins (vegetal, animal and microbial) on the degradation and allergenicity of Pru p 3. To perform this study, Pru p 3 was previously isolated using cation exchange chromatography and ultrafiltration, and the purified protein was incubated with proteases under different conditions. The results showed that only two of the fifteen proteases assayed were able to efficiently degrade the protein at acidic pH, as determined by SDS-PAGE. These two commercial acid proteases, derived from Aspergillus niger, decreased by more than 95% the immunoreactivity of Pru p 3 by ELISA using specific rabbit IgG, giving peptides lower than 3.2 kDa as determined by MALDI-TOF mass spectrometry. The hydrolysates obtained showed a greater than 70% decrease in reactivity of IgE compared to untreated Pru p 3 using three pools of sera from peach allergic individuals. Furthermore, when hydrolysates were tested by the prick test, in more than 90% of peach-allergic patients the average size of the wheal significantly decreased by between 72% and 85%. The results suggest that the acid protease from Aspergillus niger could be used to obtain novel hypoallergenic products more tolerable for peach-sensitive individuals.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0