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High throughput recurrent pregnancy loss screening: urine metabolic fingerprints via LDI-MS and machine learning

IF 4.2 3区化学

Analyst Pub Date : 2025-04-11 DOI: 10.1039/d5an00177c

Yijiao Qu, Ming Chen, Mufeng Han, Xiaoyu Yu, Xi Yu, Jinghan Fan, Huihui Liu, Liping Wang, Zongxiu Nie

{"title":"High throughput recurrent pregnancy loss screening: urine metabolic fingerprints via LDI-MS and machine learning","authors":"Yijiao Qu, Ming Chen, Mufeng Han, Xiaoyu Yu, Xi Yu, Jinghan Fan, Huihui Liu, Liping Wang, Zongxiu Nie","doi":"10.1039/d5an00177c","DOIUrl":"https://doi.org/10.1039/d5an00177c","url":null,"abstract":"Infertility is a significant challenge faced by many families worldwide, with recurrent pregnancy loss (RPL) being a prevalent cause of infertility among women. This condition causes immense emotional and physical distress for affected individuals and their families. In this study, we present a rapid, efficient, and high-throughput analytical method using PS@Fe3O4-NH2 magnetic beads as a matrix for the detection of urinary metabolite fingerprints in RPL patients via laser desorption/ionization mass spectrometry (LDI-MS) combined with machine learning (ML). This approach offers rich metabolic information from urine samples, through subsequent analysis we identify 17 metabolites that significantly differ between RPL patients and healthy controls (HC). The application of mass spectrometry features in conjunction with ML enabled effective screening of RPL patients and the identification of dysregulated metabolic pathways. This method presents a promising, non-invasive, and rapid screening approach for early detection of RPL, facilitating timely intervention and contributing to women's health.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"25 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical detection of Paracetamol based on CoO/Co3O4/NC nanocomposites derived from COFs

IF 4.2 3区化学

Analyst Pub Date : 2025-04-11 DOI: 10.1039/d5an00193e

mengjie yu, Yuting Liu, Jianhui Xiong, longfei miao, Li Wang

{"title":"Electrochemical detection of Paracetamol based on CoO/Co3O4/NC nanocomposites derived from COFs","authors":"mengjie yu, Yuting Liu, Jianhui Xiong, longfei miao, Li Wang","doi":"10.1039/d5an00193e","DOIUrl":"https://doi.org/10.1039/d5an00193e","url":null,"abstract":"Acetaminophen (APAP), also known as paracetamol, is a widely used analgesic and antipyretic, but its metabolites are toxic and can cause liver damage when used in excess. Rapid detection of APAP is essential, and conventional methods such as HPLC and GC are expensive and complex. To this purpose, we successfully prepared CoO/Co3O4/NC porous carbon composites with large specific surface area, homogeneous pore structure, and abundant adsorption active sites as electrochemical sensors for the rapid, simple, and inexpensive detection of acetaminophen. The CoO/Co3O4/NC porous carbon composites were prepared by doping Co2+ and calcining, and a large number of N and O metal chelate sites in COFTZT-DVA could coordinate with Co2+, which effectively suppressed the aggregation phenomenon of CoO/Co3O4 in the composites, and realized the uniform dispersion of Co2+. This composite material exhibits both excellent stability and catalytic performance. The experimental results showed that the sensor had an extremely low detection limit (0.79 μM) and a wide linear response range (2.5 μM-423 μM). This study provides a new strategy for the preparation of high-performance paracetamol sensors.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"4 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on cluster hollow fiber membrane proton transfer reaction mass spectrometry (CHFM-PTR-MS) and its application in odorous gas detection.

IF 3.6 3区化学

Analyst Pub Date : 2025-04-09 DOI: 10.1039/d5an00084j

Feiyang Sun, Xun Bao, Qiangling Zhang, Jie Jin, Qian Xia, Kexin Gu, Qu Liang, Wei Xu, Xue Zou, Chaoqun Huang, Chengyin Shen, Yannan Chu

{"title":"Research on cluster hollow fiber membrane proton transfer reaction mass spectrometry (CHFM-PTR-MS) and its application in odorous gas detection.","authors":"Feiyang Sun, Xun Bao, Qiangling Zhang, Jie Jin, Qian Xia, Kexin Gu, Qu Liang, Wei Xu, Xue Zou, Chaoqun Huang, Chengyin Shen, Yannan Chu","doi":"10.1039/d5an00084j","DOIUrl":"https://doi.org/10.1039/d5an00084j","url":null,"abstract":"Odorous gas emission is one of the world's seven major public nuisances, easily causing disturbances and complaints, and posing a threat to air quality and public health. Emissions of odorous substances are characterized by their sudden and instantaneous nature, with some odorous compounds having extremely low olfactory thresholds. Therefore, it is essential to develop highly sensitive on-site rapid detection techniques. This paper presents a new technology that combines a cluster hollow fiber membrane (CHFM) with mobile proton transfer reaction mass spectrometry (PTR-MS), developing a cluster hollow fiber membrane-proton transfer reaction mass spectrometry (CHFM-PTR-MS) technique. Due to the large membrane area (1000 cm2) of the cluster hollow fiber membrane module, the CHFM-PTR-MS shows improved sensitivity for eight tested odorous substances by 7.6 to 12.2 times and an enhanced limit of detection by 6.7 to 12.4 times compared to traditional direct-injection PTR-MS. Through a 28-hour continuous monitoring experiment of odorous gases released from household waste, the capability of the new CHFM-PTR-MS technology for on-site rapid detection of trace odorous organic compounds was verified. The CHFM-PTR-MS is expected to provide a new technique and device for on-site rapid detection of odorous organic compounds with high sensitivity.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-Friendly HPLC: Assessing the Sustainable Analysis of Alfuzosin, Tamsulosin, and Tadalafil Newly Approved Combinations in Organic-Solvent Free Mixed Micellar Systems

IF 4.2 3区化学

Analyst Pub Date : 2025-04-08 DOI: 10.1039/d5an00229j

Adel Ehab Ibrahim, Ahmed Al-Harrasi, Samy G. Alamir, Baher Salman, Emad Abdelhalim, Heba El Sayed

{"title":"Eco-Friendly HPLC: Assessing the Sustainable Analysis of Alfuzosin, Tamsulosin, and Tadalafil Newly Approved Combinations in Organic-Solvent Free Mixed Micellar Systems","authors":"Adel Ehab Ibrahim, Ahmed Al-Harrasi, Samy G. Alamir, Baher Salman, Emad Abdelhalim, Heba El Sayed","doi":"10.1039/d5an00229j","DOIUrl":"https://doi.org/10.1039/d5an00229j","url":null,"abstract":"In 2019, 94.00 million prevalent cases of benign prostatic hyperplasia (BPH) were reported, accounting for 2.48% of the global population. BPH is a significant urological health concern worldwide, leading to considerable economic burdens. The European and American guidelines for lower urinary tract symptoms were recently updated in 2023/2024 to support combination therapy of alpha-1 blockers with phosphodiesterase-5 inhibitors. Also, patents describing this combination were previously reported. For the first time, a totally green organic-solvent free mixed-micellar liquid chromatography method was developed to simultaneously analyze alpha-1 blockers (Alfuzosin; ALF and Tamsulosin; TMS) and phosphodiesterase-5 inhibitor (Tadalafil; TAD). Response surface methodology optimized the critical chromatographic variables, and design space representing the robustness zone was identified. Separation was achieved on RP-C18 column (150× 4.6 mm, 5 µm) using a green mixture of Brij-35 (19.91 mM), SDS (130.00 mM), and sodium dihydrogen phosphate buffer (10.00 mM) pH 4.65. The flow rate was set at 1.5 mL/min and UV detection was 250, 285 and 214 nm for ALF, TAD and TMS, respectively. The method was validated over concentration ranges of (5.00 –100.00 µg/mL) for all drugs with detection limits of 0.77, 1.23 and 1.59 µg/mL, and quantification limits of 2.35, 3.73 and 4.82 µg/mL for ALF, TAD and TMS, respectively. The method was applied to determine analytes in their prepared tablets and pharmaceutical products. Greenness was assessed using the Analytical GREEnness metric approach (AGREE) and the blue applicability grade index (BAGI) with scores of 0.76 and 82.5, respectively. These scores underline the superiority of the proposed procedure compared to the previously reported ones.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"23 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on drug-mediated protein–protein interaction in single living cells by fluorescence cross-correlation spectroscopy

IF 4.2 3区化学

Analyst Pub Date : 2025-04-08 DOI: 10.1039/d5an00021a

Wei Zhang, Xinwei Lu, Jicun Ren

{"title":"Study on drug-mediated protein–protein interaction in single living cells by fluorescence cross-correlation spectroscopy","authors":"Wei Zhang, Xinwei Lu, Jicun Ren","doi":"10.1039/d5an00021a","DOIUrl":"https://doi.org/10.1039/d5an00021a","url":null,"abstract":"Drug-mediated protein–protein interaction and drug–protein interaction form the basis of drug development and pharmacological research. How to obtain the information of drug–protein or protein–protein interaction in living cells is still a big challenge. In this work, we reported a new method for studying drug-mediated protein–protein interaction in living cells by using fluorescence cross-correlation spectroscopy (FCCS). We used the mammalian target of rapamycin (mTOR) as a model and studied drug-mediated FRB protein–FKBP12 protein interaction in living cells. The FRB protein covers amino acid residues of mTOR from 2015 to 2114 and FKBP12 is a receptor-binding protein. First, FRB was fused with the green fluorescent protein EGFP (FRB–EGFP), and FKBP12 was fused with the red fluorescent protein mCherry (FKBP12–mCherry) using genetic engineering technology. Then, FCCS was used to obtain information on drug-mediated FRB protein–FKBP12 protein interaction in living cells. According to the autocorrelation curves and cross-correlation curves, we can obtain cross-correlation (CC) values of the interaction between two proteins. The CC value was positively correlated with the interaction between two proteins. Furthermore, we developed a method for measuring IC50 for evaluating drug efficacy in living cells based on CC values. Compared with the current methods, our method can be used to study drug-mediated protein–protein interaction and evaluate effects of drugs on protein–protein interaction in living cells, and may become a useful tool for drug development and pharmacological research.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"57 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-free quantification of antibody-oligonucleotide conjugates with varying stoichiometries using capillary zone electrophoresis

IF 4.2 3区化学

Analyst Pub Date : 2025-04-07 DOI: 10.1039/d5an00214a

Mo Zhou, Xiaoxia Zhang, Qinglin Xia, Dongke Liu, Hongxu Chen, Yanli Wen, Lele Wang, Linjie Guo, Jiang Li, Lihua Wang

引用次数: 0

Donor-acceptor covalent organic framework nanofilm-based laser desorption/ionization mass spectrometry for rapid and sensitive determination of creatinine in human serum

IF 4.2 3区化学

Analyst Pub Date : 2025-04-07 DOI: 10.1039/d5an00317b

Hang Su, Zihan Chen, Juan Lin, Yanhui Zhong, Dan Ouyang, Zian Lin

{"title":"Donor-acceptor covalent organic framework nanofilm-based laser desorption/ionization mass spectrometry for rapid and sensitive determination of creatinine in human serum","authors":"Hang Su, Zihan Chen, Juan Lin, Yanhui Zhong, Dan Ouyang, Zian Lin","doi":"10.1039/d5an00317b","DOIUrl":"https://doi.org/10.1039/d5an00317b","url":null,"abstract":"Creatinine (Cre), a metabolite generated by muscles and kidneys, holds significant importance in clinical screening and detection of kidney disease. However, the existing clinical detection of Cre such as Jaffe reaction-based colorimetric method, requires complex sample pretreatment and is subject to interferences in biological samples. Herein, a surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) based on donor-acceptor covalent organic framework (D-A COF) nanofilm as a substrate was proposed for Cre determination in human serum. The D-A COF nanofilm was synthesized using the solvothermal reaction on indium-tin-oxide (ITO)-coated glass plates, which featured uniform surfaces, good thermal stability, and excellent UV absorption. Compared with conventional organic matrices, the D-A COF nanofilm-based LDI-MS method showed low background interference and high MS response, and was successfully used for the analysis of low-weight molecules such as amino acids, bisphenols, and estrogens. On this basis, the D-A COF nanofilm-based LDI-MS method was developed for the determination of Cre in human serum. The method showed good linearity in the range of 14.0-750.0 μmol/L with a low limit of detection (LOD) of 4.5 μmol/L , which could successfully cover the determination of Cre in human serum with different concentration levels. This work demonstrates the potential of this method for the clinical determination of Cre in human serum, and provides a new direction for the screening and determination of other small-molecule clinical markers.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"30 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laser Wavelength Selection in Raman Spectroscopy

IF 4.2 3区化学

Analyst Pub Date : 2025-04-07 DOI: 10.1039/d5an00324e

Mike Hardy, Hin On Martin Chu

引用次数: 0

Simultaneous magnetic purification and detection of transferrin in human serum using an imprinting-based fluorescence sensor by boronate affinity and secondary signal amplification assay 利用基于印迹的荧光传感器，通过硼酸盐亲和力和二次信号放大测定法，同时磁性纯化和检测人血清中的转铁蛋白

IF 4.2 3区化学

Analyst Pub Date : 2025-04-05 DOI: 10.1039/d5an00291e

Yuanxia Xu, Yanqiao Zhu, Jinyu Cao, Yanting Wang, Xueping Hu, Xiaohua Zhao, Xingliang Song, Lingxin Chen

{"title":"Simultaneous magnetic purification and detection of transferrin in human serum using an imprinting-based fluorescence sensor by boronate affinity and secondary signal amplification assay","authors":"Yuanxia Xu, Yanqiao Zhu, Jinyu Cao, Yanting Wang, Xueping Hu, Xiaohua Zhao, Xingliang Song, Lingxin Chen","doi":"10.1039/d5an00291e","DOIUrl":"https://doi.org/10.1039/d5an00291e","url":null,"abstract":"Transferrin (TRF) is an important glycoprotein for the early detection of disease. However, its rapid isolation and detection are challenging due to its complex structure, composition, and susceptibility to denaturation. Herein, a novel imprinting-based sensor was introduced to purify and detect TRF in human serum simultaneously. A magnetic molecularly imprinted sensor was developed by integrating boronate affinity with boric acid functionalized rodlike fluorescent porphyrin (TCPP) aggregate (A-TCPP-BA), forming a sandwich structure. When an alkaline solution (pH 10) was added, the TCPP was re-released due to disruption in the structure of A-TCPP-BA, leading to a second \"explosive\" amplification of fluorescence signals. Therefore, sensitive detection of TRF was realized with a good linear range from 2-50 μg/mL and a detection limit of 0.74 μg/mL. Furthermore, five pre-diagnosed serum samples were analyzed using this approach, obtaining recoveries in the range of 97.4% to 104.4%. In addition, the results of the significance tests (p-values of all five samples are greater than 0.05) were satisfactory compared with those of Electrochemiluminescence (ECL). Therefore, this method shows great potential for the point-of-care testing of TRF and other glycoproteins in the future.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"59 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Encapsulating Cu NCs with aggregation-induced emission into metal–organic framework ZIF-8 as a novel fluorescent nanoprobe for the highly sensitive detection of felodipine

IF 4.2 3区化学

Analyst Pub Date : 2025-04-04 DOI: 10.1039/d4an01506a

Juan Du, Huixiao Tong, Jinwen Chen, Qikun Zhang, Shenghua Liao

{"title":"Encapsulating Cu NCs with aggregation-induced emission into metal–organic framework ZIF-8 as a novel fluorescent nanoprobe for the highly sensitive detection of felodipine","authors":"Juan Du, Huixiao Tong, Jinwen Chen, Qikun Zhang, Shenghua Liao","doi":"10.1039/d4an01506a","DOIUrl":"https://doi.org/10.1039/d4an01506a","url":null,"abstract":"Fluorescent metal–organic framework nanocomposites (f-MOFs) have been gaining increasing attention in the fields of chemosensors and biosensors due to their unique signal amplification mechanisms and improved selectivity. However, most f-MOFs are constructed by encapsulating fluorescent labelling agents into frameworks via host–guest interactions. The notorious aggregation-caused quenching effect of these fluorescent labelling agents often leads to a decreased fluorescent quantum yield in f-MOFs. Herein, a novel fluorescent nanocomposite, Cu NCs@ZIF-8, was designed and prepared by encapsulating copper nanoclusters (Cu NCs) with aggregation-induced emission (AIE) effects into zeolitic imidazolate framework ZIF-8 through electrostatic attraction. Owing to the AIE effect of Cu NCs and the spatial confinement of ZIF-8, the intramolecular motion of surface ligand hydrolipidic acid (DHLA) in Cu NCs was restricted, resulting in the formation of a highly emissive nanocomposite, Cu NCs@ZIF-8. Intriguingly, the UV-Vis absorption spectrum of felodipine overlaps with the excitation spectrum of Cu NCs@ZIF-8. Therefore, a novel fluorescent nanoprobe based on Cu NCs@ZIF-8 was developed for the highly sensitive detection of felodipine via the inner-filtration effect mechanism. Under optimal detection conditions, the linear response range of Cu NCs@ZIF-8 for felodipine was found to be 1–25 μM, with a detection of limit of 0.09 μM. While determining the labelling-amount percentage in commercially available felodipine tablets, the experimental results validated that the proposed Cu NCs@ZIF-8 nanoprobe exhibits good selectivity and excellent accuracy. This expands the potential applications of fluorescent metal–organic frameworks encapsulated with metal nanoclusters exhibiting AIE properties, positioning them as fluorescent nanoprobes for pharmaceutical quality control.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"23 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0