Analyst最新文献 - Book学术

Proximity Cleavage assisted DNAzyme catalyzed Loading DNA Walker System for Accurate and Sensitive Small Extracellular Vesicles Analysis 近距离切割辅助DNAzyme催化负载DNA Walker系统精确和敏感的小细胞外囊泡分析

IF 4.2 3区化学

Analyst Pub Date : 2025-06-21 DOI: 10.1039/d5an00359h

Hongli Cao, Guofang Yin, Zhiguo Wang, Xianming Fan

{"title":"Proximity Cleavage assisted DNAzyme catalyzed Loading DNA Walker System for Accurate and Sensitive Small Extracellular Vesicles Analysis","authors":"Hongli Cao, Guofang Yin, Zhiguo Wang, Xianming Fan","doi":"10.1039/d5an00359h","DOIUrl":"https://doi.org/10.1039/d5an00359h","url":null,"abstract":"The significant biological functions and clinical importance of small extracellular vesicles (sEVs) in lung malignancies make them crucial diagnostic markers. However, the current methods for cancer related sEVs commonly recognize single molecule on the surface of sEVs, which lack enough specificity. We developed a proximity cleavage- assisted DNAzyme- catalyzed loading DNA walker system for the precise investigation of sEVs. The target sEVs served as a loading medium by utilizing the cholesterol-substrate and recognition probe to attach to the lipid bilayer and EpCAM on the surface, thereby initiating the proximity ligation-triggered DNAzyme-catalyzed DNA walker. The DNAzyme-catalyzed DNA walker enables the simultaneous detection of the lipid bilayer and EpCAM, thereby conferring high specificity to the technique. The DNAzyme-catalyzed DNA walker on the surface of sEVs and the signal generation mediated by the cleavage of free DNAzymes constitute the signal generation system. The proposed approach demonstrated a low detection limit of 2.5 particles/μL based on this design. The proposed method offers a novel approach for the sensitive detection of sEVs and other membrane structural molecules, indicating promising potential for disease diagnosis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"9 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Internal standards for 1H NMR spectroscopy in concentrated sulfuric acid. 浓硫酸中1H核磁共振光谱内部标准。

IF 4.2 3区化学

Analyst Pub Date : 2025-06-20 DOI: 10.1039/d5an00161g

Desmond B Ofosu,Robert Z Mendoza,Paul J Bracher

引用次数: 0

Two birds with one stone: A simple and integrated platform based on Fe-MOF for dual-mode detection and photocatalysis elimination of tetracycline 一石二鸟：基于Fe-MOF的简单集成平台，用于四环素的双模检测和光催化消除

IF 4.2 3区化学

Analyst Pub Date : 2025-06-19 DOI: 10.1039/d5an00433k

Fushen Niu, Hui Sun, Yifan Gao, Shusen Ding, Qing Xu, Xia Dong, Xiaoling Wang, Xiaoyan Zhang, Xiaomin Wang, Yuan Fang

{"title":"Two birds with one stone: A simple and integrated platform based on Fe-MOF for dual-mode detection and photocatalysis elimination of tetracycline","authors":"Fushen Niu, Hui Sun, Yifan Gao, Shusen Ding, Qing Xu, Xia Dong, Xiaoling Wang, Xiaoyan Zhang, Xiaomin Wang, Yuan Fang","doi":"10.1039/d5an00433k","DOIUrl":"https://doi.org/10.1039/d5an00433k","url":null,"abstract":"The persistence of tetracycline (TC) antibiotic residues in foodstuffs and aquatic systems poses critical threats to human health and ecological environment, driving an imperative demand for developing multifunctional platforms capable of simultaneous visual monitoring and high-efficiency elimination of these contaminants. Herein, a simple yet novel colorimetric sensor that integrates detection and degradation of TCs has been constructed based on the excellent peroxidase-like activity of the metal–organic frameworks (MOFs) MIL-101(Fe). Colorless 3,3′,5,5′-tetramethylbenzidine (TMB) can be effectively oxidized to generate blue oxidized TMB (oxTMB) by MIL-101(Fe), which exhibits a characteristic peak at 652 nm. The presence of TCs at varying concentrations can specifically inhibit this oxidation reaction, leading to different degrees of decrease in the intensity of the characteristic peak. Based on this concentration-dependent chromogenic behavior, TCs are quantitative identified by the dual-mode detection of UV-Vis absorbance and naked eyes. The as-fabricated colorimetric sensor displays superior selectivity toward TCs, with its detection capability in complex matrices being successfully validated in environmental water and commercial milk samples. To avoid accessional antibiotic contamination, MIL-101(Fe) can also be served as scavenger to degrade TCs efficiently under visible light irradiation, achieving up to 90.51% removal efficiency within 3 h. The developed strategy has the advantages of visual recognition, rapid field detection and no need to use large-scale instruments. Most critically, it cleverly realizes the integration of antibiotic detection and degradation, which provides a low-cost and high-efficiency solution to fundamentally solve the problem of antibiotic contamination.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"147 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144319794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solid-state nanopore quantification of discrete sequence motifs from DNA and RNA targets in human plasma 人血浆中DNA和RNA靶点的离散序列基序的固态纳米孔定量

IF 4.2 3区化学

Analyst Pub Date : 2025-06-19 DOI: 10.1039/d5an00373c

Mohamed Amin El aguech, Komal Sethi, Adam R Hall

引用次数: 0

Micro-immobilized enzyme reactors for mass spectrometry proteomics 微固定化酶反应器用于质谱分析蛋白质组学

IF 4.2 3区化学

Analyst Pub Date : 2025-06-19 DOI: 10.1039/d5an00332f

Zhongjie Yao, Yilan Li, Wei Xu

引用次数: 0

The International Society for Clinical Spectroscopy; Reflections on the first 10 years 国际临床光谱学学会；前10年的反思

IF 4.2 3区化学

Analyst Pub Date : 2025-06-19 DOI: 10.1039/d5an00419e

Malgorzata Baranska, Hugh J Byrne, Peter Gardner, Alex Henderson, Nick Stone, Bayden Robert Wood

引用次数: 0

Faster Chemical Mapping Assisted by Computer Vision: Insights From Glass and Ice Core Samples 计算机视觉辅助下更快的化学制图：来自玻璃和冰芯样品的见解

IF 4.2 3区化学

Analyst Pub Date : 2025-06-19 DOI: 10.1039/d5an00325c

Piers Larkman, Sebastiano Vascon, Martin Šala, Nicolas Stoll, Carlo Barbante, Pascal Bohleber

{"title":"Faster Chemical Mapping Assisted by Computer Vision: Insights From Glass and Ice Core Samples","authors":"Piers Larkman, Sebastiano Vascon, Martin Šala, Nicolas Stoll, Carlo Barbante, Pascal Bohleber","doi":"10.1039/d5an00325c","DOIUrl":"https://doi.org/10.1039/d5an00325c","url":null,"abstract":"Recent advances in high-repetition-rate lasers and fast aerosol transfer facilitate laser ablation in- ductively coupled plasma mass spectrometry (LA-ICP-MS) mapping rates of up to megapixels per hour, however, practical limits in time and resources still hamper mapping the chemistry of square centimetre or larger areas of target samples at high resolutions. This is especially relevant for the analysis of deep sections of polar ice cores, motivating exploration of approaches to improve the efficiency of LA-ICP-MS data collection for large-area mapping. Assisted by computer vision, and demonstrated on glass and ice samples, we show how an informed experimental design coupled with computational post processing can contribute to large reductions in measurement times and lead to associated increases in measurement areas. Using various inpainting techniques, we demonstrate how the collection of data can be reduced by up to two thirds while still capturing spatial variability. Although motivated by ice core analysis, these approaches are generalisable to other target matrices and represent a new approach to large-area LA-ICP-MS mapping.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"08 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144319740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Approach for Rapid Quantification of Food Supplement Components Using NIR Spectroscopy and Spectral Data Transfer 一种利用近红外光谱和光谱数据转移快速定量食品补充剂成分的新方法

IF 4.2 3区化学

Analyst Pub Date : 2025-06-19 DOI: 10.1039/d5an00402k

Kubra Guven, Elif Çalık Kayış, Ilkay Erdogan Orhan, Mustafa Aslan, Ismail H Boyacı, Ugur Tamer

{"title":"A Novel Approach for Rapid Quantification of Food Supplement Components Using NIR Spectroscopy and Spectral Data Transfer","authors":"Kubra Guven, Elif Çalık Kayış, Ilkay Erdogan Orhan, Mustafa Aslan, Ismail H Boyacı, Ugur Tamer","doi":"10.1039/d5an00402k","DOIUrl":"https://doi.org/10.1039/d5an00402k","url":null,"abstract":"The global interest in food supplements is increasing, creating a growing demand for efficient and reliable analytical methods to assess mixture compositions. Near-infrared (NIR) spectroscopy has been widely employed for this purpose; however, traditional chemometric approaches, such as partial least squares (PLS), require a large number of calibration samples (typically 100–200), making the process time-consuming. To overcome this limitation, we propose the spectral data transfer (SDT) approach, which corrects calculated spectra derived from pure components to more accurately align with real measured spectra. The method was tested on a four-component food supplement containing Melissa officinalis, Hypericum perforatum, Passiflora incarnata, and L-tryptophan. By implementing SDT, we significantly enhanced the prediction accuracy of PLS models, reducing RMSEP for all components. Before SDT, RMSEP values were 5.26, 7.23, 20.43 and 9.56 for Melissa officinalis, Hypericum perforatum, Passiflora incarnata, and L-tryptophan, respectively, while they were 3.43, 2.03, 2.46 and 0.86 after SDT and preprocessing (2nd derivative) respectively. Validation using HPLC reference analysis confirmed the accuracy, robustness, and repeatability of the proposed method, demonstrating its effectiveness in advancing NIR spectroscopy for mixture analysis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"147 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144319971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dye doped fluorescent polypyrrole nanotherapeutic probes as a versatile platform for ratiometric pH sensing-guided and self-monitored photothermal therapy of tumors 染料掺杂荧光聚吡咯纳米治疗探针作为比值pH传感引导和自我监测肿瘤光热治疗的通用平台

IF 4.2 3区化学

Analyst Pub Date : 2025-06-19 DOI: 10.1039/d5an00426h

Bei Li, Ruyu Li, Qi Zan, Xiaojing Chai, Xincheng Sun, Xiaoran Zhang, Chuan Dong, Shaomin Shuang

{"title":"Dye doped fluorescent polypyrrole nanotherapeutic probes as a versatile platform for ratiometric pH sensing-guided and self-monitored photothermal therapy of tumors","authors":"Bei Li, Ruyu Li, Qi Zan, Xiaojing Chai, Xincheng Sun, Xiaoran Zhang, Chuan Dong, Shaomin Shuang","doi":"10.1039/d5an00426h","DOIUrl":"https://doi.org/10.1039/d5an00426h","url":null,"abstract":"A multifunctional fluorescence polypyrrole nanotherapeutic probe (denoted as PPy@RhB-PAA-FITC) was constructed for pH sensing-guided photothermal therapy (PTT) of cancer and self-monitoring temperature variations during PTT. By using photothermal therapy materials polypyrrole (PPy) nanoparticles as a matrix, the temperature-responsive rhodamine B (RhB) and pH-sensitive fluorescein isothiocyanate (FITC) fluorescent dye were linked to the nanoparticles through encapsulation and electrostatic interaction, respectively. The fluorescent intensity of FITC increased dramatically with enhanced pH and little variation appeared in emission intensity of RhB, which was responsible for ratio fluorescent pH sensing performance. For pH detection, the nanoprobes exhibited a linearity in pH range from 4.54 to 6.4 with an acid dissociation constant (pKa) value of 5.80. PPy nanoparticles were in charge of PTT that endowed this nanotherapeutic system with 36.24% photothermal conversion efficiency. Meanwhile, the fluorescent intensity of RhB was decreased due to the increase of heat-induced temperature, which enabled to satisfy the need of self-monitoring temperature during PTT. The thermal sensitivity of 1.4%/°C were harvested in temperature-sensing assays. Moreover, by the combination of the ratio fluorescent pH imaging, the photothermal conversion and self-detection temperature, the PPy@RhB-PAA-FITC was successfully applied for pH-mediated tumor diagnosis and self-monitored treatment. The proposed PPy@RhB-PAA-FITC broadens the PPy-based nanomaterials applications in ratiometric fluorescent pH imaging and self-feedback temperature changes guided photothermal therapy.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"604 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144319987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal-organic frameworks (MOFs) based separation columns: fundamental study for molecular recognitions and potential for separation of linear polymers with close terminal structure 基于金属有机框架（MOFs）的分离柱：分子识别的基础研究和具有紧密末端结构的线性聚合物的分离潜力

IF 4.2 3区化学

Analyst Pub Date : 2025-06-18 DOI: 10.1039/d5an00548e

Keigo Matsubara, Yoshiyuki Watabe, Sayaka K-Yamada, Nobuhiko Hosono, Takashi Uemura, Takuya Kubo

{"title":"Metal-organic frameworks (MOFs) based separation columns: fundamental study for molecular recognitions and potential for separation of linear polymers with close terminal structure","authors":"Keigo Matsubara, Yoshiyuki Watabe, Sayaka K-Yamada, Nobuhiko Hosono, Takashi Uemura, Takuya Kubo","doi":"10.1039/d5an00548e","DOIUrl":"https://doi.org/10.1039/d5an00548e","url":null,"abstract":"We report stationary phases using metal-organic frameworks (MOFs) composed of metal ions and organic ligands in HPLC. We mainly applied [Zn2(1,4-ndc)2ted]n (ZnJAST4) (ndc: naphthalenedicarboxylate, ted: triethylenediamine) for the stationary phase. To inspect how metal ions and organic ligands affect retention behavior, three different types of MOF, [Cu2(1,4-ndc)2ted]n (CuJAST4) substituted from zinc ions of ZnJAST4 to copper ions, [Zn2(1,4-bdc)2ted]n (ZnJAST1) (bdc: benzenedicarboxylate) substituted from a part of naphthalene of ZnJAST4 to benzene, and ZnJAST4 were packed to columns, and then HPLC using benzene derivatives was conducted to evaluate the recognition ability toward functional groups and shapes of molecules using hexane as the mobile phase. The result revealed that the retention behavior depended on various factors such as halogen bonding, π stacking, molecular shapes, and molecular sizes. Furthermore, to evaluate retention selectivity toward functional groups, HPLC analyses were conducted with a ZnJAST4 packed column in a mobile phase, N,N-dimethylformamide (DMF), using poly(ethylene glycol) (PEG) derivatives, which have different polar terminal groups, as analytes. The result showed that when the polarity was low, the retention to ZnJAST4 decreased, and the retention behavior was affected by the molecular weight of the PEG.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"15 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0