AnalystPub Date : 2025-01-14DOI: 10.1039/d4an01162g
Qiang Xu, Huiting Yin, Ze Zhao, Mei Cui, Renliang Huang, Rongxin Su
{"title":"Au-Ag@Au fiber surface plasmon resonance sensor for highly sensitive detection of fluoroquinolones residue","authors":"Qiang Xu, Huiting Yin, Ze Zhao, Mei Cui, Renliang Huang, Rongxin Su","doi":"10.1039/d4an01162g","DOIUrl":"https://doi.org/10.1039/d4an01162g","url":null,"abstract":"Antibiotic residue detection plays an important role in protecting human health, but real-time, rapid, and highly sensitive detection is still challenging. Herein, gold and silver nanoparticles (Au-Ag NPs) were in situ grown on the surface of optical fibers and a 50-nm thick gold film was deposited on the sensor's surface to fabricate Au-Ag@Au fiber SPR sensor. The sensitivity of the sensor reached 3512 nm/RIU in the refractive index range of 1.328-1.371. When analyzing antibiotic residues in food, the detection limits for Enro and Cip were 0.97 ng mL-1 and 0.70 ng mL-1, respectively. The recoveries of them ranged from 96.64-115.32% and 96.18-121.06% in milk samples. There is a high level of antibiotic residues in meat, milk, and aquatic products and this study has significant potential in food safety testing applications. Moreover, this study will be widely used in environmental monitoring (such as heavy metal ions), medical diagnosis (viruses), marine exploration, drug screening and other analytical fields as well.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"74 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rapid Identification of Pathogenic Bacteria from Clinic Positive Blood Cultures Via Virus-like Magnetic Bead Enrichment and MALDI-TOF MS Profiling","authors":"Zhirou Zhang, Enyun Xing, Wenzhuo Zhao, Minghui Song, Cuiping Zhang, Hong Liu, Xiaomin Li, Hongxiu Yu","doi":"10.1039/d4an01424c","DOIUrl":"https://doi.org/10.1039/d4an01424c","url":null,"abstract":"Reducing the time required for the detection of bacteria in blood samples is a critical area of investigation in the field of clinical diagnosis. Positive blood culture samples often require a plate culture stage due to the interference of blood cells and proteins, which can result in significant delays before the isolation of single colonies suitable for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. In this study, we developed a non-specific enrichment strategy based on SiO<small><sub>2</sub></small>-encapsulated Fe<small><sub>3</sub></small>O<small><sub>4</sub></small> nanoparticles combined with MALDI-TOF MS for direct identification of bacteria from aqueous environments or positive blood culture samples. Three distinct types of Fe<small><sub>3</sub></small>O<small><sub>4</sub></small>@SiO<small><sub>2</sub></small> magnetic nanoparticles (MNPs) with unique surface morphologies were developed: spherical MNPs with smooth surfaces (Fe<small><sub>3</sub></small>O<small><sub>4</sub></small>@SN), mesoporous silica coated MNPs (Fe<small><sub>3</sub></small>O<small><sub>4</sub></small>@MSN), and MNPs exhibiting a viral spiked structure (Fe<small><sub>3</sub></small>O<small><sub>4</sub></small>@VSN). These MNPs exhibited excellent binding affinity towards both <em>Staphylococcus aureus</em> and <em>Klebsiella pneumoniae</em> in PBS and artificial saliva solutions. Furthermore, the use of the Fe<small><sub>3</sub></small>O<small><sub>4</sub></small>@VSN strategy, which involves non-specific interactions between bacterial cells and the virus-like surface, resulted in a dramatic reduction in the minimum detectable concentrations of target pathogens by up to 1000-fold compared to conventional methods. Our results demonstrate that the Fe<small><sub>3</sub></small>O<small><sub>4</sub></small>@VSN strategy has the potential to significantly reduce the processing time required after blood culture and may be useful for enrichment and identification of microorganisms in complex clinical samples.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnalystPub Date : 2025-01-14DOI: 10.1039/d4an01375a
Shanshan Du, Ziyan Liu, Lei Wu, Fangbiao Tao
{"title":"Identification and characterization of microplastics released during actual use of disposable cups using laser direct infrared imaging","authors":"Shanshan Du, Ziyan Liu, Lei Wu, Fangbiao Tao","doi":"10.1039/d4an01375a","DOIUrl":"https://doi.org/10.1039/d4an01375a","url":null,"abstract":"Disposable plastic cups are commonly used as beverage containers. This study investigated the characteristics of microplastics (MPs) discharged from plastic cups in everyday situations and assessed the emission of MPs from disposable cups, including polypropylene (PP) and polystyrene (PS). We systematically examined the impact of the material, temperature, and liquid type on the release of MPs using laser direct infrared (LDIR) spectroscopy. The findings indicated that all cups released of irregularly shaped MPs smaller than 50 μm. When filled with water at 95°C for 15 min, the average MPs in the PP and PS cups were 1340 and 980 particles/L, respectively. The disposable plastic cups exhibited higher average MPs values at elevated temperatures. Similar profiles were observed for the two types of cups, in which the MP release capacity at 95°C was 1.5 times that at 50°C. Low temperatures facilitated the self-release of MPs from disposable cups. Furthermore, reusable disposable cups decreased MP release compared with single-use cups. The study estimated that regular use of a cup three times a day could lead to the unintentional ingestion of 294-402 MPs per day. These results provide a basis for evaluating possible health hazards associated with human contact with MPs.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"24 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnalystPub Date : 2025-01-14DOI: 10.1039/d4an01520g
Hamada A. A. Noreldeen, Chen-Ting Zhu, Kai-Yuan Huang, Huaping Peng, Hao-Hua Deng, Wei Chen
{"title":"Double Probes-based Fluorescence Sensor Array to Detect Rare Earth Element Ions","authors":"Hamada A. A. Noreldeen, Chen-Ting Zhu, Kai-Yuan Huang, Huaping Peng, Hao-Hua Deng, Wei Chen","doi":"10.1039/d4an01520g","DOIUrl":"https://doi.org/10.1039/d4an01520g","url":null,"abstract":"There is a persistent need for effective sensors to detect rare earth element ions (REEIs) due to their effects on human health and the environment. Thus, a simple and efficient fluorescence-based detection method for REEIs that offers convenience, flexibility, versatility, and efficiency is essential for ensuring environmental safety, food quality, and biomedical applications. In this study, 6-aza-2-thiothymine-gold nanoclusters (ATT-AuNCs) and bovine serum albumin/3-mercaptopropionic acid-AuNCs (BSA/MPA-AuNCs) were utilized to detect 14 REEIs (Sc3+, Gd3+, Lu3+, Y3+, Ce3+, Pr3+, Yb3+, Dy3+, Tm3+, Sm3+, Ho3+, Tb3+, La3+, and Eu3+), resulting in the creation of a simple, sensitive, and multi-target fluorescence sensor array detection platform. We observed that REEIs exert various enhancement or quenching effects on ATT-AuNCs and BSA/MPA-AuNCs. Thus, these two probes function as double signal channels, with the different effects of REEIs serving as signal inputs. Pattern recognition methods, including hierarchical cluster analysis (HCA) and linear discriminant analysis (LDA), were used to assess the recognition performance of the constructed sensing system. Beyond the excellent ability to recognize individual REEIs, the platform is also capable of distinguishing mixed REEIs. Also, this approach was validated by applying it to detect REEIs in purified water samples. This method not only minimizes the need for synthesizing and optimizing new probes but also offers a novel approach for the determination and identification of diverse analytes, filling a gap in the detection of a large number of REEIs simultaneously.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"22 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnalystPub Date : 2025-01-14DOI: 10.1039/d4an01497a
Yan Liu, Xue Kong, Yi Zhang, Xiumei Zhou, Zheng-Zhi Yin
{"title":"An electrochemical microsensor of the SARS-CoV-2 nucleocapsid protein based on surface-imprinted acupuncture needle","authors":"Yan Liu, Xue Kong, Yi Zhang, Xiumei Zhou, Zheng-Zhi Yin","doi":"10.1039/d4an01497a","DOIUrl":"https://doi.org/10.1039/d4an01497a","url":null,"abstract":"A novel electrochemical microsensor is constructed on a traditional acupuncture needle (AN) and used to monitor a biomarker of the SARS-CoV-2-N protein. The reversible interaction of the borate bond between the cis-diol in this glycoprotein and the phenylboronic acid in 4-mercaptophenylboronic acid (4-MPBA) is effectively exerted. This interaction was applied to anchor the SARS-CoV-2-N protein onto 4-MPBA, which is covalently self-assemblied onto electrodeposited AuNPs by the S-Au bond. Meldola blue was then electropolymerized around the protein template. After the template was eluting, three-dimensional nanocavities complementary with the protein were generated within the polymelola blue (pMB) layer. Interestingly, nanocavities could play a channel role for the electron-transfer of outer [Fe(CN)6]3−/4−, and the signal of electrochemical probe could be hindered after recombining the SARS-CoV-2-N protein, which laid a platform for the detection of this biomarker. After optimizing the influencing factors, the prepared microsensor exhibits a linear range of 0.1 ~ 1000 ng mL−1 with a low detection limit of 0.01 ng mL−1 (S/N = 3). In particular, the sensing ability was dramatically affected by the thickness correlative factors for the polymer matrix. The suitable thickness is effective for the sensing signals, which corresponds to the behavior of surface-imprinted polymer. The microsensor showed comparatively high sensitivity and selectivity and practically detected the SARS-CoV-2-N protein in the serum sample, which is of scientific significance for the development of the electrochemical microsensor and acupuncture.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rationally engineering recognition group to construct a two-photon reaction-based fluorescent probe for rapid and selective sensing of cysteine","authors":"Ziwei Zhang, Zhen Shi, Yumeng Yang, Junyong Sun, Feng Gao","doi":"10.1039/d4an01468e","DOIUrl":"https://doi.org/10.1039/d4an01468e","url":null,"abstract":"It is highly requested to rationally design fluorescent probes with desired analytical performances for the applications in sensing and imaging through molecular engineering strategy. The reaction-based fluorescent probes for highly selective sensing of cysteine (Cys) are mainly based on Cys-participated addition-cyclization with acrylates, cyclization with aldehydes, coordination displacement, Michael addition reaction, and cleavage reaction. Cys-triggered reaction with “O” ether bond has also been used to construct the reaction-based fluorescent probe based on the substitution of the ether with the nucleophilic thiolate of Cys. However, many of the developed probes still suffer from long response time, interfering from homocysteine (Hcy) and glutathione (GSH), high background fluorescence, and lack of two-photon absorption (TPA) properties. Herein, we successfully design a Cys-sensitive two-photon fluorescent probe (F-BTD) using a donor-acceptor-donor (D-A-D) type π-extended benzothiadiazole framework as the fluorophore, while nitrobenzofuran (NBD) as the recognition unit. The proposed F-BTD probe displays some advantages including rapid response, high selectivity, low background, and two-photon imaging capability. The F-BTD probe is applied to two-photon fluorescence imaging of endogenous and exogenous Cys in HeLa cells with satisfactory results. For comparing, commonly used recognition groups of biothiol including 2,4-dinitrobezensulfonyl and 2,4-dinitrophenyl are also used to construct S-BTD and N-BTD probes, respectively. The responsive mechanism of F-BTD to Cys is studied in detail through the kinetic studies and transition-state analysis. This study may provide an example to design fluorescent probe with desired analytical performances by taking recognition group into consideration as an important index.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"137 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnalystPub Date : 2025-01-13DOI: 10.1039/d4an01208a
Felix Sughnen Atsar, Hillary Donna Bourger, Christopher Anthony Baker
{"title":"Online Integration of Capillary Electrophoresis and Dual Detector Taylor Dispersion Analysis via a 3D Printed Instrument","authors":"Felix Sughnen Atsar, Hillary Donna Bourger, Christopher Anthony Baker","doi":"10.1039/d4an01208a","DOIUrl":"https://doi.org/10.1039/d4an01208a","url":null,"abstract":"Hydrodynamic radius (R<small><sub>H</sub></small>) is a descriptive metric of protein structure with the potential to impact drug development, disease diagnosis, and other important research areas of molecular biology. Common instrumental methods for molecular size characterization are disadvantageous due to high sample consumption, measurements made in non-physiological conditions, and/or inaccurate size determinations. Capillary Taylor dispersion analysis (TDA) is a molecular sizing method that utilizes nL sample volumes and achieves absolute size determination without calibration or comparison to standards. One key drawback of TDA is that it reports the concentration-weighted average R<small><sub>H</sub></small>, which may be limiting in the analysis of complex sample mixtures. Here, we describe the development of a 3D printed instrument to integrate capillary electrophoresis (CE) separations online with TDA size characterization. Dual laser-induced fluorescence detectors were developed to enable two-channel detection using a single PMT and fluorescence filter set, achieving detection limits for AlexaFluor 532 of to 0.6 ± 0.4 nM and 1.1 ± 0.2 nM for detectors 1 and 2, respectively. Joule heating during CE separations was observed to introduce bias in subsequent TDA measurements. The effects of Joule heating were mitigated by integrating a water circulating sheath flow on the portion of the capillary used for CE. The utility of CE-TDA in bioanalysis was demonstrated by standard-free peak identification in the ficin digestion of IgG1. CE-TDA was further applied to characterizing denaturation dynamics of the Group II heat resistant protein apolipoprotein A-1 (ApoA), in which R<small><sub>H</sub></small> was observed to increase from 2.3 ± 0.2 nm at 20 °C to 5.2 ± 0.5 nm while heated at at 90 °C, then returned to a quasi-native state with R<small><sub>H</sub></small> = 2.9 ± 0.5 nm after cooling to 20 °C. CE-TDA is a powerful analysis mode with potential to impact various domains of bioanalysis. The instrument developed in this work offers a low barrier to entry for researchers interested in adopting this methodology","PeriodicalId":63,"journal":{"name":"Analyst","volume":"22 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnalystPub Date : 2025-01-13DOI: 10.1039/d4an01340a
Duyeop Kim, Do Yeon Kim, Ji Hoon Han
{"title":"Development of Novel Fluorescent Light-up Pb2+ Sensor Using G-quadruplex Complex with Modified Thioflavin T","authors":"Duyeop Kim, Do Yeon Kim, Ji Hoon Han","doi":"10.1039/d4an01340a","DOIUrl":"https://doi.org/10.1039/d4an01340a","url":null,"abstract":"We developed a novel, cost-effective fluorescent light-up biosensor for Pb²⁺ detection using a label-free G-quadruplex combined with modified Thioflavin T (ThT) derivatives, utilizing the unique K⁺-Pb²⁺-G-quadruplex conformation. Among them, the T2 G-quadruplex with ThT-E showed the best fluorescence enhancement, validating its high sensitivity and selectivity in real water samples","PeriodicalId":63,"journal":{"name":"Analyst","volume":"19 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrasensitive detection of microRNAs based on cascade amplification strategy of RCA-PER and Cas12a","authors":"Xue Li, Dongxia Wang, Zhifeng Wei, Qingbo Xu, Jiahong Wang, Wenhui Zhang, Anling Zhang, Chuanjing Ju","doi":"10.1039/d4an01463d","DOIUrl":"https://doi.org/10.1039/d4an01463d","url":null,"abstract":"Since microRNAs (miRNAs) serve as markers for early cancer diagnosis, it is crucial to develop a novel biosensor to detect miRNAs quickly, sensitively and selectively. Hence, we developed a fluorescence biosensor, based on target miRNA-initiated rolling circle amplification (RCA) to generate RCA products with multiple tandem catalytic hairpin DNA templates that trigger primer exchange reactions (PER) which extend short single-strand DNA (ssDNA) primers into long ssDNA. Subsequently, the long ssDNA activates the trans-cleavage activity of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system to cleave a fluorescent reporter chain, enabling ultrasensitive detection of miRNAs through the output fluorescence signal. The biosensor could quantify miRNA-141 concentrations from 100 to 105 pM, with a detection limit of 94 fM. Therefore, the biosensing strategy proposed in this study offers a robust technique for the clinical diagnosis of miRNA-141.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"84 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnalystPub Date : 2025-01-08DOI: 10.1039/d4an01121j
Mangmang Shen, Chang Ni, Jiasheng Yuan, Xin Zhou
{"title":"Phage-ELISA for ultrasensitive detection of Salmonella enteritidis","authors":"Mangmang Shen, Chang Ni, Jiasheng Yuan, Xin Zhou","doi":"10.1039/d4an01121j","DOIUrl":"https://doi.org/10.1039/d4an01121j","url":null,"abstract":"The M13 phage carries approximately 5 copies of the pIII protein, each of which is capable of displaying a single-chain variable fragment (scFv) that targets a specific antigen. This feature enables the M13 phage to be widely employed in the construction of scFv libraries, thereby facilitating the identification of antibodies with high specificity and affinity for target antigens. In this study, mice were immunized three times with Salmonella enteritis (strain C50041) to induce a diverse antibodies. The variable region sequences were subsequently amplified by PCR using genome extracted from the mice’s splenic cells and fused to the pIII protein to construct the scFv phage display library (C50041-M13-scFv). Through biopanning with the C50041-M13-scFv library, a phage clone (C50041-scFv-4) exhibiting high affinity to the target bacteria was successfully obtained. Moreover, the scFv antibody (scFv-4) derived from C50041-scFv-4 was expressed in a prokaryotic expression system and validated to possess high specificity and affinity for C50041 through in vitro adsorption assays. Additionally, a phage-ELISA method was established: initially, bacteria were immobilized on the bottom surface of a 96-well plate. Next, the positive clone C50041-scFv-4 was introduced to specifically bind to the host cells. Finally, horseradish peroxidase (HRP)-conjugated anti-pⅧ antibodies were used to detect the pⅧ proteins of the bound phage clones. Owing to the capacity of multiple C50041-scFv-4 probes to simultaneously bind to a single target Salmonella and each phage clone’s ability to accommodate hundreds of HRP-labeled antibodies, the proposed phage-ELISA demonstrated remarkable sensitivity (10^4 CFU/mL) for detecting Salmonella enteritidis samples. This sensitivity surpasses traditional ELISA by one order of magnitude in this study. Our phage-ELISA technology exhibits broad applicability across various biological species and provides an improved and robust platform for pathogen detection including bacteria and viruses.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"9 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}