Analyst最新文献 - Book学术

A Centrifugal Microfluidic Chip with its Companion Device for Automatic Point-of-Care Nanozyme ELISA Lateral Flow Immunoassay Based on Optoelectronic Sensing 一种离心式微流控芯片及其配套装置用于基于光电传感的自动护理点纳米酶ELISA侧流免疫分析

IF 4.2 3区化学

Analyst Pub Date : 2025-05-30 DOI: 10.1039/d5an00237k

Ming Liu, Lei Wang, Jiaxi Zhou, Guijun Miao, Lulu Zhang, Lizeng Gao, Xianbo Qiu

{"title":"A Centrifugal Microfluidic Chip with its Companion Device for Automatic Point-of-Care Nanozyme ELISA Lateral Flow Immunoassay Based on Optoelectronic Sensing","authors":"Ming Liu, Lei Wang, Jiaxi Zhou, Guijun Miao, Lulu Zhang, Lizeng Gao, Xianbo Qiu","doi":"10.1039/d5an00237k","DOIUrl":"https://doi.org/10.1039/d5an00237k","url":null,"abstract":"Influenza virus spread rapidly, seriously threatening human health, for example, causing serious and even deadly respiratory diseases, and weakening the global economy. Influenza virus detection methods include molecular detection and immunoassay, and compared to molecular detection, the traditional lateral flow immunoassay is widely used in point-of-care testing (POCT) due to its advantages of fast detection, low cost and ease of use. However, these assays are often limited by trivial operational procedure, specialized skills, and long detection time. To address these challenges and enable rapid and highly sensitive pathogen detection, we developed a centrifugal analyzing platform and a centrifugal microfluidic chip integrated with nanozyme lateral flow strip for automated detection of H1N1 viral antigens. The signal amplification using nanozyme based enzyme-linked immunosorbent assay (ELISA) significantly improves the sensitivity and shortens the assay time. A peak searching algorithm was utilized to semi-quantitatively analyze the detection and control lines on the lateral flow strip of the nanozyme, which showed a 2.5-fold increase in the lower limit of detection compared to the eye-observation-based assay. In the automated detection system, the centrifugal microfluidic chip sequentially delivers the sample and color development solution to the lateral flow strip, facilitating fully automated operation and minimizing the risk of manual errors. Experimental results show that the manual operation takes only 1 minute, and the total testing time is only 16 minutes. This study highlights a multifunctional platform for automated nanozyme-based ELISA assay, offering broad applicability in medical diagnostics, environmental monitoring, and food safety testing.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"352 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocatalysis on Plastic Stick Tips: Atypical Electrochemical Enzyme Biosensing on Adjacent Bare Working Electrode Disks 塑料棒尖端的生物催化：相邻裸工作电极盘上的非典型电化学酶生物传感

IF 4.2 3区化学

Analyst Pub Date : 2025-05-29 DOI: 10.1039/d5an00510h

H. Sasimali M. Soysa, Kawin Loha, Wipa Suginta, Albert Schulte

引用次数: 0

A validated experimental NMR parameter dataset of organic molecules to assist benchmarking of 3D structure determination methods. 一个经过验证的有机分子实验核磁共振参数数据集，以协助对标三维结构确定方法。

IF 4.2 3区化学

Analyst Pub Date : 2025-05-29 DOI: 10.1039/d5an00240k

Claire Louise Dickson, Sadia Mohammed, Lyrelle S. L. Jones, Duncan J Crick, Wojciech Augustyniak, Michael Beaumont, Jan-Christoph Westermann, Sarah Henshaw, Steven T Johnston, Zoltan Takacs, Charles Blundell, Martin Watson, Craig Philip Butts

{"title":"A validated experimental NMR parameter dataset of organic molecules to assist benchmarking of 3D structure determination methods.","authors":"Claire Louise Dickson, Sadia Mohammed, Lyrelle S. L. Jones, Duncan J Crick, Wojciech Augustyniak, Michael Beaumont, Jan-Christoph Westermann, Sarah Henshaw, Steven T Johnston, Zoltan Takacs, Charles Blundell, Martin Watson, Craig Philip Butts","doi":"10.1039/d5an00240k","DOIUrl":"https://doi.org/10.1039/d5an00240k","url":null,"abstract":"Over 1000 accurately defined and validated experimental long-range proton-carbon (nJCH) and proton-proton (nJHH) scalar coupling constants, with assigned 1H/13C chemical shifts and their corresponding 3D structures are reported for fourteen relatively complex organic molecules. The experimental dataset comprises 775 nJCH, 300 nJHH, 332 1H chemical shifts and 336 13C chemical shifts, all validated against DFT-calculated values to identify misassignments. A subset of 565 nJCH, 205 nJHH, 172 1H chemical shifts and 202 13C chemical shifts from rigid portions of these molecules have been identified which could be particularly valuable for benchmarking computational methods for predicting NMR parameters. An exemplar application of this dataset is presented through benchmarking of the DFT mPW1PW91/6-311g(dp) level of theory for computation of chemical shifts and scalar coupling constants and for testing scaling approaches for generating experimentally-relevant chemical shifts from DFT-computed magnetic shielding tensors.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"58 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144165442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A portable turbidity-based sensor constructed via inhibition of lipase activity for point-of-use testing of dichlorvos pesticide 通过抑制脂肪酶活性构建便携式敌敌畏农药使用点检测浊度传感器

IF 4.2 3区化学

Analyst Pub Date : 2025-05-29 DOI: 10.1039/d5an00311c

Jingyi Qiu, Xinyu Xiao, Yiting Ou, Lei Han, Shi Gang Liu

{"title":"A portable turbidity-based sensor constructed via inhibition of lipase activity for point-of-use testing of dichlorvos pesticide","authors":"Jingyi Qiu, Xinyu Xiao, Yiting Ou, Lei Han, Shi Gang Liu","doi":"10.1039/d5an00311c","DOIUrl":"https://doi.org/10.1039/d5an00311c","url":null,"abstract":"Point-of-use testing techniques are very significant in the fields like food safety and environmental monitoring. Here, we propose a portable turbidity-based sensor for point-of-use quantification of dichlorvos pesticide (DDVP) via inhibition of lipase activity to regulate the formation of fatty acid calcium precipitate. Typically, the fatty acid produced by Candida antarctica lipase B (CaLB)-catalytic hydrolysis of Tween-20 can combine with calcium ions to form precipitate, accompanied by heightened turbidity. DDVP can effectively inhibit the activity of CaLB, resulting in reduced turbidity. The concentration of DDVP is thereby determined by monitoring the variation of turbidity using a portable nephelometer. The sensing strategy is applied to quantitative analysis of DDVP in food samples with satisfying results. The portable nephelometer is handheld, user-friendly, and cost-effective and also all reagents are commercially accessible. As a result, the proposed turbidity-based sensor has great potential for point-of-use detection of DDVP in practical applications. This work exploiting CaLB-mediated turbidity as sensing readout signal also provides a new insight to develop point-of-use testing techniques.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"5 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144165443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-step seed swelling polymerization to prepare poly(glycidyl methacrylate-divinylbenzene) microspheres and their sulfonation for chromatographic separation of rare earth elements 两步种子膨胀聚合法制备聚甲基丙烯酸缩水甘油酯-二乙烯基苯微球及其磺化反应用于稀土元素色谱分离

IF 4.2 3区化学

Analyst Pub Date : 2025-05-28 DOI: 10.1039/d5an00388a

Chen Shen, Yuqing Wei, Shuaishuai Wang, Hongwei Zhao, Qifang He, Hongdeng Qiu

引用次数: 0

DNA tetrahedron-inspired magnetic hybrid with regular distribution of trypsin for ultra-fast digestion of proteins and glycoproteomics analysis DNA四面体激发磁杂交与规则分布的胰蛋白酶超快速消化蛋白质和糖蛋白组学分析

IF 4.2 3区化学

Analyst Pub Date : 2025-05-28 DOI: 10.1039/d5an00357a

Jiahui Lai, Danni Wang, Xiuqin Sheng, Chuanfan Ding, Yinghua Yan

{"title":"DNA tetrahedron-inspired magnetic hybrid with regular distribution of trypsin for ultra-fast digestion of proteins and glycoproteomics analysis","authors":"Jiahui Lai, Danni Wang, Xiuqin Sheng, Chuanfan Ding, Yinghua Yan","doi":"10.1039/d5an00357a","DOIUrl":"https://doi.org/10.1039/d5an00357a","url":null,"abstract":"In large-scale glycoproteomics studies, reducing the experimental duration and enhancing the digestion efficiency are crucial for the experiments. In this work, a magnetic nanomaterial featuring a surface rich in hydrophilic groups and uniformly distributed trypsin (Fe3O4@DOPA/PEI@Au@DNA TET-Trypsin) was developed for efficient digestion of proteins and specific enrichment of glycopeptides. The material surface exhibits a regular distribution of trypsin coupled with abundant hydrophilic groups. Thus, the material can demonstrate excellent performance in experimental studies related to glycoproteomics. Fe3O4@DOPA/PEI@Au@DNA TET-Trypsin showed an excellent limit of detection (0.001 amol·μL-1) and high selectivity for glycopeptides (BSA:HRP=5000:1) in a short reaction time, and it showed good performance in the processing of practical samples. Gene Ontology (GO) analysis suggests that complement activation, extracellular space, antioxidant activity and complement binding may be associated with tuberculosis. These analytical results provide new insights for determining and clarifying the pathogenesis and pathology related to tuberculosis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"3 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-free Diagnosis of Lung Cancer by Fourier Transform Infrared Microspectroscopy Coupled with Domain Adversarial Learning 傅里叶变换红外微光谱与领域对抗学习相结合的肺癌无标记诊断

IF 4.2 3区化学

Analyst Pub Date : 2025-05-28 DOI: 10.1039/d5an00216h

Yudong Tian, Xiangyu Zhao, Jingzhu Shao, Bingsen xue, Lianting Huang, Yani Kang, Hanyue Li, Gang Liu, Haitang Yang, Chongzhao Wu

{"title":"Label-free Diagnosis of Lung Cancer by Fourier Transform Infrared Microspectroscopy Coupled with Domain Adversarial Learning","authors":"Yudong Tian, Xiangyu Zhao, Jingzhu Shao, Bingsen xue, Lianting Huang, Yani Kang, Hanyue Li, Gang Liu, Haitang Yang, Chongzhao Wu","doi":"10.1039/d5an00216h","DOIUrl":"https://doi.org/10.1039/d5an00216h","url":null,"abstract":"Lung cancer is one of the most prevalent malignancies, characterized by high morbidity and mortality rates. Current diagnostic approaches primarily rely on CT imaging and histopathological evaluations, which are time-consuming, heavily dependent on pathologists' expertise, and prone to misdiagnosis. Fourier transform infrared (FTIR) microspectroscopy is a promising label-free technique that can offer insights into morphological and molecular pathologic alterations from biological tissues. Here, we present a novel FTIR microspectroscopic method enhanced by a deep learning model for differentiating lung cancer tissues, which serves as a crucial adjunct to clinical diagnosis. We propose an Infrared Spectral Domain Adversarial Neural Network (IRS-DANN), which employs a domain adversarial learning mechanism to mitigate the impact of inter-patient variability, thereby enabling the accurate discrimination of lung cancer tissues. This method demonstrates superior classification performance on a real clinical FTIR dataset, even with limited training samples. Additionally, we visualize and elucidate the FTIR fingerprint peaks, which are linked to the corresponding biological components and crucial for lung cancer differentiation. These findings highlight the great potential of incorporating FTIR microspectroscopy with the deep learning model as a valuable tool for the diagnosis and pathological studies of lung cancer.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"57 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of airborne metal-containing nanoparticles in a historic mining area using single particle ICP-MS 用单颗粒ICP-MS测定历史矿区空气中含金属纳米颗粒

IF 4.2 3区化学

Analyst Pub Date : 2025-05-27 DOI: 10.1039/d5an00480b

Tjaša Žerdoner, Janja Vidmar, Bor Arah, Tea Zuliani

{"title":"Determination of airborne metal-containing nanoparticles in a historic mining area using single particle ICP-MS","authors":"Tjaša Žerdoner, Janja Vidmar, Bor Arah, Tea Zuliani","doi":"10.1039/d5an00480b","DOIUrl":"https://doi.org/10.1039/d5an00480b","url":null,"abstract":"Given limited information on airborne metal-containing NPs—a highly bioaccessible fraction of metals relevant to human exposure—in the upper Meža Valley, a historic mining area in Slovenia, this study aimed to assess their presence in PM10 air filters from the region using the spICP-MS method. The extraction procedure, refined using a certified reference material of PM10-like fine dust deposited on filters, achieved an extraction efficiency of 9.1% for Zn- and 14.0% for Pb-containing NPs after two hours of ultrasonication in 10 mM sodium pyrophosphate. The method proved effective for detecting metal-containing NPs in PM10 from mining and smelting areas, as demonstrated by a case study from the upper Meža Valley. SpICP-MS analyses identified both Zn- and Pb-containing NPs in PM10 samples, with 0.6–3.8% of Zn and 0.3–1.7% of Pb extracted as NPs. Additionally, SEM-EDS analysis confirmed the presence of Zn- and Pb-containing (nano)particles of different chemical compositions. This study is the first to report the occurrence of metal-containing NPs in PM10 from this region. Although they represent only a small fraction of total Zn and Pb in the samples, NPs are more bioaccessible and thus more relevant for assessing local population's exposure to these particles.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"59 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144145672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-sensitive cavity ring-down spectroscopy for hydroperoxyl radical measurement based on tunable narrow-linewidth laser using linear cavity optical feedback 基于线性腔光反馈的可调谐窄线宽激光器的高灵敏度腔衰荡光谱对羟基自由基的测量

IF 4.2 3区化学

Analyst Pub Date : 2025-05-27 DOI: 10.1039/d5an00260e

Yang Chen, Weixiong Zhao, Bo Fang, Nana Yang, Nuo Chen, Weijun Zhang

{"title":"High-sensitive cavity ring-down spectroscopy for hydroperoxyl radical measurement based on tunable narrow-linewidth laser using linear cavity optical feedback","authors":"Yang Chen, Weixiong Zhao, Bo Fang, Nana Yang, Nuo Chen, Weijun Zhang","doi":"10.1039/d5an00260e","DOIUrl":"https://doi.org/10.1039/d5an00260e","url":null,"abstract":"The hydroperoxyl radical (HO2) is an important oxidant, playing a crucial role in atmospheric chemistry processes such as free radical cycling and ozone formation. However, the direct measurements of HO2 using laser absorption techniques present significant challenges due to its low atmospheric concentration, high reactivity, and short lifetime. In this paper, a high-sensitive narrow linewidth cavity ring-down spectroscopy (CRDS) system for HO2 detection was developed. By employing dual locking of feedback phase and laser current to the resonant cavity, the probe laser linewidth was successfully narrowed from 2.3 MHz to 12.3 kHz and the frequency noise was reduced by 4 to 5 orders of magnitude. Through cavity length adjustments, a continuous laser frequency tuning range of 4.5 GHz was achieved. This narrow linewidth laser significantly heightened trigger efficiency of the CRDS system. The Allan deviation analysis at a fixed laser frequency demonstrated that an improved sensitivity of 3.24×10-11 cm-1 could be obtained with an averaging time of 0.48 s, which corresponded to an HO2 detection limit of 1.29×108 molecule/cm3. The developed system featured a simple design and provided a valuable technical reference for future research on direct HO2 measurements.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"56 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144145468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multi-modal mass spectrometry approach for the detection and mapping of date rape drugs in fingermarks 多模态质谱法在手印中检测和定位枣奸药

IF 4.2 3区化学

Analyst Pub Date : 2025-05-27 DOI: 10.1039/d5an00328h

R. Krishna, K. Hamer, R. Bradshaw, S. Bleay, L. M. Cole, E. Claude, J. Langridge, J. Bucek, S. Francese

{"title":"A multi-modal mass spectrometry approach for the detection and mapping of date rape drugs in fingermarks","authors":"R. Krishna, K. Hamer, R. Bradshaw, S. Bleay, L. M. Cole, E. Claude, J. Langridge, J. Bucek, S. Francese","doi":"10.1039/d5an00328h","DOIUrl":"https://doi.org/10.1039/d5an00328h","url":null,"abstract":"Although drug facilitated sexual assault (DFSA) is an old issue, current statistics on the frequency of this crime have led to growing concerns. As these drugs are metabolised very quickly, toxicological evidence from biological fluids, corroborating the victim's statement, is challenging to recover, especially with late reports. We are proposing an additional method involving the analysis of the victim's fingermarks recovered at the scene(s) of the crime, which may contain the parent drug and its metabolite. As a case study, a multi-modal mass spectrometry-based approach has been developed and explored to detect and image both risperidone and its pharmacologically active metabolite, paliperidone, after contamination of a fingertip at very low concentrations and deposition of a fingerprint on a surface. In particular Matrix Assisted Laser Desorption Ionisation Mass Spectrometry Imaging (MALDI MSI), Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI MSI) and Soft Ionisation by Chemical Reaction In Transfer, (SICRIT®) have been used in different combinations to both detect the drugs and reconstruct the fingerprint ridge pattern; this approach enables the simultaneous provision of both chemical information (circumstances surrounding the crime) and biometric information. A forensic operational scenario has also been simulated whereby the contaminated fingerprint is deposited on paper and enhanced with a routine fingerprint enhancement technique prior to analysis via mass spectrometry imaging. Overall, our investigation indicates that this additional approach is feasible and is worth exploring further.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"133 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144145674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0