Analyst最新文献

{"title":"Sensitive aptasensing of tobramycin through a rational design of catalytic hairpin assembly and hybridization chain reaction amplification monomers for CRISPR/Cas12a activation","authors":"Bei Lv, Pengda Liang, Chenhao Chang, Dawei Li","doi":"10.1039/d5an00267b","DOIUrl":"https://doi.org/10.1039/d5an00267b","url":null,"abstract":"The catalytic hairpin assembly (CHA) and hybridization chain reaction amplification (HCR) are enzyme-free isothermal DNA amplification methods based on the self-assembly of hairpin monomers. Recently, CRISPR/Cas12a-based biosensors in combination with CHA or HCR signal amplification have shown promising performance. Herein, several design strategies for hairpin monomers in CHA and HCR were evaluated in the context of CRISPR/Cas12a-based biosensor construction. The SL-HCR strategy, in which the CRISPR/Cas12a target strand is blocked in the loop of one hairpin monomer DNA and released in the duplex HCR products, demonstrated superior performance in terms of a low background signal, wide linear detection range, and high signal-to-noise ratio. With the assistance of an aptamer-containing probe, a highly sensitive aptasensor was constructed for tobramycin detection, whereby the SL-HCR served the function of signal amplification, whereas the CRISPR/Cas12a system acted to cleave the FQ probes, thereby resulting in the production of a fluorescent signal. After optimization, the aptasensor enables linear detection of tobramycin concentrations ranging from 125 pM to 2500 nM, with a limit of detection (LOD) of 92.87 pM. Moreover, the aptasensor was utilized to detect tobramycin in beef and milk samples, yielding satisfactory results. The assay is concise and cost-effective due to the absence of nanomaterial DNA labeling and magnetic separation procedures. Furthermore, the entire detection workflow operates under isothermal conditions, which makes it suitable for use in food safety control and environmental monitoring. In addition, the results presented here may shed new light on the design of CRISPR/Cas12a-based biosensors in combination with CHA or HCR.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"26 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143920919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequential detection of H₂S and HClO fluorescence probes and their applications in real sample detection, smartphone sensing development, and bioimaging","authors":"Zeqian Liu, Yinuo Yan, Hui Wang, Xiaodong Wang, Bingchun Xue","doi":"10.1039/d5an00382b","DOIUrl":"https://doi.org/10.1039/d5an00382b","url":null,"abstract":"Hydrogen sulfide (H2S) and hypochlorous acid (HClO) are typical environmental pollutants and bioactive substances, and their detection has become increasingly important. A highly effective method for real-time monitoring of H₂S and HClO is to utilize a fluorescent probe to detect changes in their levels. In this study, a dual-site chemosensor BPCN-SSPy, based on 2,2'-dithiodipyridine benzoate and dicyanobis(isophorone)ene, was developed for sequential detection of H2S and HClO. The sensor enabled “turn-on” detection of H₂S in its fluorescence channel (λem: 611 nm), followed by ratiometric detection of HClO (λem: F520 nm/F611 nm), demonstrating rapid response and high selectivity, and was used for detecting them in real water samples. Notably, the sequential color change response of H₂S and HClO using BPCN-SSPy opened up a smartphone sensing platform. BPCN-SSPy demonstrated excellent biocompatibility and can be used for the visualization and imaging of H2S in HeLa cells. Additionally, the solid-state luminescent properties of BPCN-SSPy can make it suitable in potential fingerprint recognition applications.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"48 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143920917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring the Kinetic Evolution of Mesenchymal Stem Cell Differentiation using Raman Micropectroscopy","authors":"Francesca Ravera, Esen Efeoglu, Hugh J Byrne","doi":"10.1039/d4an01509f","DOIUrl":"https://doi.org/10.1039/d4an01509f","url":null,"abstract":"Raman microspectroscopy (RMS) offers a powerful, non-destructive approach for in situ monitoring of dynamic biochemical processes within cells. However, the ability to reliably data-mine the spectroscopic signatures and their evolution and extract meaningful information can be challenging. Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) regression analysis is a powerful chemometric technique that can potentially address this challenge by deconvoluting the spectra into individual component spectra, each representing a specific biochemical species, and regressing the solutions against kinetic constraints. In this study, MCR-ALS analysis was performed on spectral data of differentiation process of mesenchymal stem cells into chondrocytes, carried out on two different substrates, collagen 3-dimensional hydrogel and the conventional 2D culture model at time intervals of 1-7, 14, 21 days. The kinetic evolution of the chondrogenesis was modelled according to a phenomenological rate equation model, in an attempt to describe the biochemical evolution of the cell composition within the process. Moreover, the ability of the algorithm to faithfully extract the correct reaction rates and spectral profiles has been explored. The results indicated that the differentiation process originates in the nucleolar regions, subsequently extending to the nuclear and cytoplasmic compartments and corroborated a more rapid differentiation rate in cell cultures grown on 3D collagen hydrogels compared to 2D substrates. The combination of Raman microspectroscopy and MCR-ALS offers a powerful approach for elucidating the complex mechanisms underlying chondrogenesis and developing innovative strategies for regenerative medicine.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"37 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143920918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of plasticizers using thermal desorption dielectric barrier discharge ionization mass spectrometry","authors":"Xiaokang Guan, Xue You, Renato Zenobi, Qiao Lu","doi":"10.1039/d5an00327j","DOIUrl":"https://doi.org/10.1039/d5an00327j","url":null,"abstract":"Plasticizers, which are extensively utilized in the manufacturing of plastic products, have garnered significant attention due to their potential toxicity and the consequent health and environmental risks they pose. Traditional methods for detecting plasticizers, such as gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS), are often time-consuming and require complex sample preparation. In response to these challenges, this study introduces an innovative approach for the rapid and sensitive identification of seven common plasticizers using thermal desorption dielectric barrier discharge ionization mass spectrometry (TD-DBDI-MS). The proposed technique significantly reduces background interference by employing nitrogen as the discharge gas, thereby enabling the detection of characteristic quasi-molecular ions ([M+H]+) and their fragments, which are crucial for structural elucidation. The method exhibits remarkable sensitivity, with a limit of detection as low as 0.1 ppm for diethyl phthalate and a linear dynamic range of orders of magnitude. Furthermore, it allows for expedited screening of various plastic products, including cling wrap, package bags, and centrifuge tubes, with each sample analyzed in less than 30 seconds. This study underscores the efficacy of TD-DBDI-MS as a rapid, sensitive, and user-friendly methodology for the preliminary screening of plasticizers in a wide range of materials.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"2 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyphenated Mass Spectrometry Methods for Enlarged Capacity Data Storage Systems based on Chemical Mixtures","authors":"Cristian Vicent Barrera, Victor Sans, Raquel Cerveró, Victor Flors, Cristopher Tinajero","doi":"10.1039/d5an00353a","DOIUrl":"https://doi.org/10.1039/d5an00353a","url":null,"abstract":"Encoding abstract information in chemical mixtures uses the selective presence or absence of specific analytes, creating a binary-based framework for data storage. Data storage capacity (C in bits) can be maximized by encoding with large analyte libraries (M) at distinguishable concentration levels (L), where C = M·log2L. However, roboust decoding of such complex libraries remains challenging for practical applications. This study introduces hyphenated Mass Spectrometry (MS) methods, Liquid Chromatography (LC) and Flow Injection Analysis (FIA) that meet the dual requirements of high analyte coverage and precise quantitation to maximize data storage capacity. Encoding and decoding use plant metabolite libraries to create specific mixtures. Using LC-MS, it is feasible to encode and decode up to 200 bits per mixture, with scalability reaching 10³-10⁴ bits at the cost of low decoding rates (ca. 0.5 bits / sec). FIA-MS offers a high-throughput alternative, handling 100 bits at faster rates (ca. 3 bits / sec). The data storage capacity can be three-fold expanded by incorporating up to eight quantitation levels, supporting binary, quaternary, or octal encoding schemes. To demonstrate the practical application of these methods, we encode and decode various digital file formats such as texts and multicolor images.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"20 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomaterial-modified electrochemical aptasensor for tetracycline detection: A review","authors":"Jingjing Huo, Rui Guo, Juanjuan Yin, Yanran Liu, Ying Zhang, Fangyi Ruan, Yonghong Shi, Yang Li","doi":"10.1039/d5an00097a","DOIUrl":"https://doi.org/10.1039/d5an00097a","url":null,"abstract":"Excessive residues of tetracyclines in the livestock, food products and environment can accumulate in the human body through the food chain, unavoidably posing threat to human health. Therefore, it is essential to establish detection methods with high specificity, stability, and sensitivity. Among the numerous detecting techniques, electrochemical sensors with aptamer working as biorecognition elements have been increasingly applied to monitor tetracycline. Notably, the synergy of a wide range of nanomaterials with aptamers-based sensors could improve the charge transfer efficiency and signal sensitivity. In this review, the advantages of aptamer-based recognition method are discussed and the measuring process of electrochemical detection are introduced. Then, advances in electrochemical aptasensor used for detecting tetracycline are summarized with emphasis on the role of nanomaterials functioning as transducing media, such as carbon-based nanomaterials, gold-based nanomaterial and electrically conductive polymer. At the end, we also discuss the current challenges and emerging trends in this field, shedding light on the prospects for developing new aptasensor for tetracycline detection.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"75 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of AuPt@ZIF-67 nanomaterials and their application in flow injection chemiluminescence immunoassay","authors":"Yufeng Zhou, Longyue Wang, Xiao Mei, Jingjing Xi, Kang Wu, Jianguo Li","doi":"10.1039/d5an00405e","DOIUrl":"https://doi.org/10.1039/d5an00405e","url":null,"abstract":"Compared to natural enzymes, which are prone to deactivation, require expensive purification processes, and have stringent storage demands, nanozymes demonstrate superior stability, cost-efficiency, and customizable catalytic performance. In this study, the AuPt@ZIF-67 nano-enzyme composites with high catalytic activity were constructed by loading Au and Pt nanoparticles onto the zeolitic imidazolate framework-67 (ZIF-67). Capitalizing on the porous structure of ZIF-67 and the electronic synergy between the Au-Pt bimetallic components, the material demonstrated exceptional peroxidase-like activity. Harnessing the engineered properties of this advanced nanomaterial, a flow-injection chemiluminescence immunoassay (FI-CLIA) platform was engineered to achieve ultrarapid and ultrasensitive monitoring of aflatoxin B1 (AFB1) in foodstuffs. Experimental results indicated a linear dynamic range of 0.002~1000 ng mL-1 for AFB1 quantification, achieving an ultralow detection limit of 0.68 pg mL-1 (S/N = 3). The spiked recovery rates for corn and wheat samples ranged from 81.5% to 110.3%. The findings indicated that this method exhibits significant applicability prospects for AFB1 detection in actual sample analysis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"46 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectroscopic chiral discrimination of naproxen isomers with cellulose tris(3,5-dimethylphenylcarbamate) modified mesoporous hollow polymer microspheres","authors":"Nan Shi, Shuyu Li, Laidi Xu, Junyao Li, Wenrong Cai, Datong Wu, Yong Kong","doi":"10.1039/d5an00408j","DOIUrl":"https://doi.org/10.1039/d5an00408j","url":null,"abstract":"Mesoporous hollow polymer microspheres (M-HPMs) are modified with cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC), and the obtained chiral hollow polymer microspheres (C-HPMs) are utilized for the spectroscopic chiral discrimination of naproxen (NPX) isomers. Thanks to the intrinsic chirality of CDMPC and the mesoporous structure of M-HPMs, the NPX isomers exhibit significant differences in their absorbances on the ultraviolet (UV) spectra after being adsorbed by the C-HPMs. Several parameters including adsorption time, mass ratio of CDMPC to M-HPMs, and NPX concentration are optimized to achieve the highest discrimination efficiency for the NPX isomers. Compared with the M-HPMs modified with sodium carboxymethyl cellulose and the hollow polymer microspheres (HPMs) directly modified with CDMPC, the C-HPMs show higher discrimination efficiency for the NPX isomers.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"23 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid screening of antioxidants in biodegradable plastics by surface-assisted laser desorption ionization mass spectrometry","authors":"Muyi He, Meixia Xu, Yun Ling, Wei Yong, Minli Yang, Wei Guo","doi":"10.1039/d5an00368g","DOIUrl":"https://doi.org/10.1039/d5an00368g","url":null,"abstract":"Antioxidants are added into biodegradable plastics to prevent polymer degradation caused by oxidation, thereby prolonging the lifespan and maintaining the physical properties of plastics. However, during the degradation process, additives may be released into the environment or food, posing risks of pollution and health hazards. In this study, a surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) method was developed for rapid identification and quantification of antioxidants in biodegradable plastics. Graphene oxide (GO) was utilized as an ionization assistant to enhance ionization efficiency and minimize background interference. The method was optimized using four common antioxidants (1098, 1520, 1726, and 3114), achieving reliable identification through accurate mass measurements and tandem MS (MS/MS) analysis. Dynamic range curves were derived from standard solutions with determination coefficients exceeding 0.989. The performance of the method was also evaluated using authentic samples and commercially available biodegradable plastic products with recovery rates ranging from 78% to 109%, and limit of detection is 5.0 g kg<small>⁻¹</small>. Sample preparation is straightforward and the entire analytical process can be completed within just a few minutes. This method is suitable for rapid qualitative detection of additives in biodegradable plastics as well as quantitative evaluation of substance content variations during plastic degradation. It shows promise as a valuable tool for quality control and safety assurance in the plastic industry.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"19 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Detection of Multiple Gas Mixtures and Evaluation of Harmful Gas Removal Efficiency in Deck Decompression Chamber Using Dynamic Switching Mass Spectrometry","authors":"Qu Liang, Pingxiao Liu, Lei Zhao, Xuejun Wang, Jun Zou, Xun Bao, Qiangling Zhang, Wei Xu, Xue Zou, Shifeng Wang, Chaoqun Huang, 成银 沈, Yannan Chu","doi":"10.1039/d5an00132c","DOIUrl":"https://doi.org/10.1039/d5an00132c","url":null,"abstract":"The multiple gas mixtures in the deck depression chamber (DDC) serves as a life-sustaining medium for divers to adapt to high-pressure environments and to safely decompress after saturation diving operations. However, due to the complexity of the gas composition and the wide concentration range, there is currently no technique that can rapidly detect all multiple gases in the DDC. In this study, we developed a photoinduced chemical ionization (pCI) and electron impact (EI) dynamic switching mass spectrometry. This technique was applied to investigate the spatial distribution uniformity of respiratory gases within the DDC at pressures of 0.13 MPa, 0.6 MPa, and 1.5 MPa, as well as the removal efficiency of toxic gases at 1.5 MPa. The switching stability time of the pCI/EI dynamic switching mass spectrometry was 30 s/circle, with optimal detection performance metrics including: detection limit down to 0.58 ppb (H₂S), fastest response time of 2 s (for CO₂ and H₂S), sensitivity up to 62.97 counts/ppb (H₂S), and an overall concentration detection range spanning from 1.0×10⁻⁹ v/v (1 ppb) to 4.5×10⁻¹ v/v (45%), covering the requirements for all target gases. At the three pressure levels, the spatial distribution uniformity of O2 in the DDC was 0.34%, 0.21%, and 0.08%, with the time to reach uniform distribution being 5 min, 3 min, and 1 min, respectively. For CO2, the spatial distribution uniformity was 0.62%, 1.61%, and 1.30%, with uniform distribution achieved in 10 min, 7 min, and 5 min, respectively. The removal efficiencies for harmful gases such as CO2, CH4, NH3, and H2S were 39.57%, 10.75%, 14.29%, and 3.96%, respectively. Under high-pressure conditions, O2 and CO2 rapidly achieved uniform distribution within the DDC. The pCI/EI dynamic switching mass spectrometry technology provides a rapid detection method for multicomponent gases in enclosed chambers like the DDC. This technology holds significant implications for the life support and operational efficiency of personnel involved in submarine rescue, shipwreck salvage, underwater construction, and deep-sea exploration of marine resources.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"281 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}