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Novel Au-anchored Fe-NC nanozyme enabled rapid and sensitive colorimetric detection of Cr(VI) 新型au -锚定Fe-NC纳米酶快速灵敏比色检测Cr（VI）

IF 4.2 3区化学

Analyst Pub Date : 2025-10-02 DOI: 10.1039/d5an00830a

Shumin Xi, Fengjun Luo, Peng Chen, Ding Wang, Jingxi Wang, Beibei Kou, Renyong Zhao

{"title":"Novel Au-anchored Fe-NC nanozyme enabled rapid and sensitive colorimetric detection of Cr(VI)","authors":"Shumin Xi, Fengjun Luo, Peng Chen, Ding Wang, Jingxi Wang, Beibei Kou, Renyong Zhao","doi":"10.1039/d5an00830a","DOIUrl":"https://doi.org/10.1039/d5an00830a","url":null,"abstract":"Hexavalent chromium (Cr(VI)), as a high biologically toxic heavy metal ion, poses a great threat to environmental safety and human health. Herein, we report a facile and cost-effective colorimetric sensing platform for Cr(VI) detection based on Au nanoparticles-anchored Fe-NC nanozyme (AuNPs/Fe-NC) catalysis. The AuNPs/Fe-NC nanozyme shows comparable catalytic activity and kinetics to natural horseradish peroxidase (HRP), which could catalyze 3,3’,5,5’<img alt=\" \" border=\"0\" src=\"https://www.rsc.org/images/entities/char_200a.gif\"/>-tetramethylbenzidine (TMB) to its oxidized form with a blue color. In the presence of 8-hydroxyquinoline (8-HQ), the catalytic activities of prepared AuNPs/Fe-NC was inhibited, which weaken AuNPs/Fe-NC-catalyzed oxidation towards TMB. After Cr(VI) was introduced, this catalytic activity of AuNPs/Fe-NC nanozyme could be recovered, resulting in an increasing blue color. By monitoring the variation of UV-vis absorbance, Cr(VI) had been quantified analysis and shown excellent sensitivity with the detection limit as low as 0.25 μM, and the linear range was 1-165 μM. It also exhibited high selectivity for a series of metal cations, and had been successfully applied to the detection of Cr(VI) in tap water.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"1 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on the Extraction of Aging Characteristics from Diethylene glycol dinitrate Propellant Using RGB Image Processing 基于RGB图像处理的硝酸二甘醇推进剂老化特性提取研究

IF 4.2 3区化学

Analyst Pub Date : 2025-10-02 DOI: 10.1039/d5an00818b

Yingyi Wu, Yanchun Li, Qian Guo, Yang Chen, Dongming Song, Aifeng Jiang

{"title":"Research on the Extraction of Aging Characteristics from Diethylene glycol dinitrate Propellant Using RGB Image Processing","authors":"Yingyi Wu, Yanchun Li, Qian Guo, Yang Chen, Dongming Song, Aifeng Jiang","doi":"10.1039/d5an00818b","DOIUrl":"https://doi.org/10.1039/d5an00818b","url":null,"abstract":"Diethylene glycol dinitrate(DEGDN) propellant, as a key material for the power source of weapon systems, the research on its aging characteristics is of great significance for ensuring the safety and reliability of weapons. This study systematically analyzed the thermal reaction mechanism of DEGDN propellant using the thermogravimetry-differential scanning calorimetry-quadrupole mass spectrometry (TG-DSC-QMS), and investigated the changes in the components of DEGDN propellant under humidity and temperature aging conditions by using high-performance liquid chromatography (HPLC). The results show that during the humidity and temperature aging process, the main aging characteristics of the DEGDN propellant are the continuous loss of nitrate ester plasticizers. Among them, the consumption rate of DEGDN is the fastest, followed by nitroglycerin. Further research revealed a significant linear correlation between the color change of the extraction solution and the loss of components in the propellant. Based on this, this study established a quantitative relationship model between the content of DEGDN and the RGB parameters of the propellant solution, providing a new idea and feasible research direction for the rapid detection of propellant components.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"114 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast, general-purpose metabolome analysis by mixed-mode liquid chromatography – mass spectrometry 快速，通用代谢组分析混合模式液相色谱-质谱

IF 4.2 3区化学

Analyst Pub Date : 2025-10-02 DOI: 10.1039/d5an00641d

Mario S.P. Correia, Alaa Othman, Nicola Zamboni

引用次数: 0

Detection of Carbapenemase-Mediated Antimicrobial Resistance using Surface-Enhanced Raman Scattering 利用表面增强拉曼散射检测碳青霉烯酶介导的抗菌素耐药性

IF 4.2 3区化学

Analyst Pub Date : 2025-10-02 DOI: 10.1039/d5an00839e

Ziying Wang, Hridaynath Bhattarcharjee, Mitchell Jeffs, Rachel A V Gray, Yazan Bduor, Aristides Docoslis, Christopher T Lohans, Carlos Escobedo

引用次数: 0

Calcium-Doped Lanthanum Cobaltite-based Electrochemical Sensor for Sensitive Detection of Salicylic Acid in Real Samples: Pond Water and Acne Gels 基于钙掺杂钴酸镧的电化学传感器用于真实样品中水杨酸的灵敏检测：池塘水和痤疮凝胶

IF 4.2 3区化学

Analyst Pub Date : 2025-10-01 DOI: 10.1039/d5an00760g

Chinchu Gibi, Sambandam Anandan, Cheng-Hua Liu, Jerry Wu

{"title":"Calcium-Doped Lanthanum Cobaltite-based Electrochemical Sensor for Sensitive Detection of Salicylic Acid in Real Samples: Pond Water and Acne Gels","authors":"Chinchu Gibi, Sambandam Anandan, Cheng-Hua Liu, Jerry Wu","doi":"10.1039/d5an00760g","DOIUrl":"https://doi.org/10.1039/d5an00760g","url":null,"abstract":"Salicylic acid (SA) is a kind of Contaminants of Emerging Concern (CECs) belonging to the category of pharmaceutical products. It is commonly used as drug in treatment of various skin diseases and as an over-the-counter (OTC) drug used in an undisciplined manner across the world. It is also used as a supplement for yield improvement in plants. Exposure to high doses of SA can lead to a toxic condition as known as Salicylism. To better monitor the levels of SA, Ca doped LaCoO 3 (LCCO)/GCE is fabricated in this study. CLCO is synthesized by solvothermal method and characterised by SEM, FE-TEM, XRD, EDX, and XPS techniques.Substitution of Ca 2+ with La 3+ results in formation of oxygen vacancies due to variation in oxidation states of Co. The similar ionic radius of La 3+ and Ca 2+ deliver better structural stability as well as better catalytic activity for the perovskite. LCCO/GCE with its excellent catalytic activity could detect SA with the limit of detection (LOD) of 30 nM and a linear range of 0.05-800 µM. The fabricated sensor is also reproducible with relative standard deviation (RSD) less than 1% and stable up to 30 days with less than 5% variation from the initial current value. The sensor is also selective for sensing SA even in presence of hundred-fold concentrations of other interferents. In addition, the real-world applicability of the sensor is investigated in pond water and acne gel which both give excellent recovery percentages.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"3 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cascade Signal Amplification-Based Fluorescent Biosensor Utilizing Exonuclease III and Self-Locking DNAzyme Synergy for microRNA Detection 基于级联信号放大的荧光生物传感器，利用外切酶III和自锁DNAzyme协同作用进行microRNA检测

IF 4.2 3区化学

Analyst Pub Date : 2025-09-30 DOI: 10.1039/d5an00810g

Daqi Chen, Zhezhi Fang, Yachunyue Zhou, Ziyao Zeng, Jiancong Liang, Baian Zhu, Chaozhan Chen

{"title":"Cascade Signal Amplification-Based Fluorescent Biosensor Utilizing Exonuclease III and Self-Locking DNAzyme Synergy for microRNA Detection","authors":"Daqi Chen, Zhezhi Fang, Yachunyue Zhou, Ziyao Zeng, Jiancong Liang, Baian Zhu, Chaozhan Chen","doi":"10.1039/d5an00810g","DOIUrl":"https://doi.org/10.1039/d5an00810g","url":null,"abstract":"MicroRNAs (miRNAs), as pivotal regulators of gene expression, are critically associated with the progression of cancer, neurodegenerative, and cardiovascular diseases. However, their detection remains challenging due to low sensitivity, background noise, and matrix interferences in complex biological samples, hindering clinical applications such as early tumor screening and liquid biopsy. Herein, we present a cascade-amplified fluorescence biosensor driven by the synergistic integration of exonuclease III (Exo III) and self-locking DNAzyme (SLD). This strategy exploits Exo III-mediated cyclic digestion of target-induced DNA/RNA heteroduplexes to release multiple signal conversion chains, while the SLD’s rigid conformational gate suppresses nonspecific catalysis until unlocked by target-specific molecular keys. The activated DNAzyme then drives secondary amplification through iterative cleavage of fluorophore-labeled reporters. This dual-amplification mechanism achieves label-free, isothermal detection of miRNA-155 with a linear range of 50 fM–10 pM and a limit of detection (LOD) of 4.96 fM. Notably, the sensor demonstrates robust specificity in serum matrices, effectively overcoming homologous miRNA cross-reactivity and complex biological interferences. By merging target recycling with conformation-gated catalysis, this platform offers a cost-effective, high-precision solution for miRNA profiling, holding significant promise for point-of-care diagnostics and liquid biopsy-based early cancer detection.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"91 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A microfluidic sample preparation and droplet SERS detection all-in-one device for online analysis of deltamethrin in meat products 肉制品中溴氰菊酯在线分析微流控制样及液滴SERS检测一体化装置

IF 4.2 3区化学

Analyst Pub Date : 2025-09-30 DOI: 10.1039/d5an00787a

Jianying Huang, Yayue Huang, Ling Xia, Xiaohua Xiao, Gongke Li

{"title":"A microfluidic sample preparation and droplet SERS detection all-in-one device for online analysis of deltamethrin in meat products","authors":"Jianying Huang, Yayue Huang, Ling Xia, Xiaohua Xiao, Gongke Li","doi":"10.1039/d5an00787a","DOIUrl":"https://doi.org/10.1039/d5an00787a","url":null,"abstract":"Surface-enhanced Raman spectroscopy (SERS) is a powerful tool to acquire the fingerprint information of molecules rapidly, sensitively, and noninvasively. Its application in sample analysis, however, suffers from low repeatability due to the interference of the sample matrix. To address this issue, a microfluidic device was developed to realize all-in-one sample preparation and SERS detection for the online analysis of deltamethrin in meat products. This device was constructed using a microfluidic sample preparation unit and a droplet SERS detection unit. Through flow rate control, analytes at appropriate concentrations can be continuously encapsulated into online-generated microdroplets with SERS substrate Ag nanoparticle-coated Au nanoparticles. Stronger intensities with smaller relative standard deviations were observed by accumulating the Raman signals of microdroplets. Using the microfluidic SERS device, the enhancement factors of rhodamine 6G and deltamethrin were 2.7 × 107 and 3.8 × 105, respectively, with RSDs less than 4.0% (n = 19). Moreover, this device can be reused more than 7 times through simple cleaning procedures. The established microfluidic SERS analytical method has a linear range of 30.0–500 μg L−1 with a correlation coefficient of 0.9953, and the limit of detection was 11.6 μg L−1 (S/N = 3). The established microfluidic SERS method was applied to deltamethrin analysis in chicken skin, chicken and grass carp; 32.8 μg kg−1 of deltamethrin was found in the chicken skin sample. The accuracy and precision of the method were confirmed by high-performance liquid chromatography and recovery test results, indicating its potential for application in food safety.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"94 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Additively manufactured microplate for the simultaneous colorimetric and electrochemical detection of atropine 用于同时比色和电化学检测阿托品的增材制造微孔板

IF 4.2 3区化学

Analyst Pub Date : 2025-09-30 DOI: 10.1039/d5an00897b

Larissa M. A. Melo, Elena Bernalte, Robert D. Crapnell, Matthew J. Whittingham, Rodrigo A. A. Munoz, Wallans T. P. dos Santos, Craig E. Banks

{"title":"Additively manufactured microplate for the simultaneous colorimetric and electrochemical detection of atropine","authors":"Larissa M. A. Melo, Elena Bernalte, Robert D. Crapnell, Matthew J. Whittingham, Rodrigo A. A. Munoz, Wallans T. P. dos Santos, Craig E. Banks","doi":"10.1039/d5an00897b","DOIUrl":"https://doi.org/10.1039/d5an00897b","url":null,"abstract":"This work presents the development of a dual mode electrochemical and colorimetric sensing platform, produced in a single print through additive manufacturing. The cell design was based on the dimensions of a standard 96-well plate, with the base replaced by a disc electrode made from bespoke conductive polypropylene for the electrochemical testing, with the walls created from transparent non-conductive polypropylene to allow for the colorimetric tests. This new system was employed for the detection of atropine (ATP) in two distinct steps within the same electrochemical cell: (1) colour changes due to the reaction of ATP with bromocresol green, allowing for preliminary visual identification, and (2) the analysis of the electrochemical behaviour of the system before and after the colour change, providing quantitative confirmation. Both steps were performed in the same cell, highlighting the efficiency and practicality of the developed device. Wide linear ranges were obtained using square-wave voltammetry for ATP detection, spanning 0.65 to 20.83 mg mL−1 before the colorimetric reaction, and 5.21 to 20.83 mg mL−1 after the colorimetric reaction. Detection and quantification limits were calculated as 0.15 mg mL−1 and 0.50 mg mL−1, respectively, demonstrating suitability for real application in forensic scenarios. Beverage samples (energy drink, tonic water, gin, gin with tonic water, and whisky) and synthetic biological samples (saliva, urine, and vitreous humour) were spiked with ATP and analysed using the proposed method, yielding recoveries close to 100%, indicating no matrix effect. This study demonstrates the synergy between additive manufacturing, and electrochemical and colorimetric sensing to create real, functional sensing platforms that are applicable to a wide range of fields.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"11 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical Investigation of Intracellular NADH: PASHs Effect on Mitochondrial Function 细胞内NADH的电化学研究：PASHs对线粒体功能的影响

IF 4.2 3区化学

Analyst Pub Date : 2025-09-29 DOI: 10.1039/d5an00993f

Liping Lu, kang chunyu

{"title":"Electrochemical Investigation of Intracellular NADH: PASHs Effect on Mitochondrial Function","authors":"Liping Lu, kang chunyu","doi":"10.1039/d5an00993f","DOIUrl":"https://doi.org/10.1039/d5an00993f","url":null,"abstract":"Nicotinamide adenine dinucleotide (NADH) is a crucial biomolecule that serves as an essential cofactor in numerous biocatalytic reactions and a pivotal regulator of mitochondrial function and cellular physiology. This study developed a SECM platform utilizing a double-mediator system for the real-time, in situ, and non-invasive detection of intracellular NADH at the single living cell level. The system employs 1-methoxy-5-methylphenazinium methyl sulfate (mPMS), which permeates the cell membrane to oxidize intracellular NADH, and ferricyanide ([Fe(CN)₆]³⁻), which shuttles electrons from the reduced mPMS to the SECM tip. This methodology was applied to investigate mitochondrial impairment in human hepatoma (HepG2) cells induced by polycyclic aromatic sulfur heterocycles (PASHs). The metabolic response was corroborated by analyzing poly(ADP-ribose) polymerase (PARP) activity, adenosine triphosphate (ATP) content, and mitochondrial membrane potential (MMP). Results demonstrated that exposure to dibenzothiophene (DBT) activated PARP, leading to severe depletion of NADH and ATP and a significant decline in MMP, thereby confirming comprehensive mitochondrial dysfunction. Comparative analysis with its non-sulfur analog fluorene (Flu) revealed that the sulfur atom in DBT's structure is a critical determinant of its enhanced cytotoxicity. This work establishes a reliable and innovative analytical approach for assessing the subcellular metabolic effects of environmental pollutants, providing a new platform for in-depth cytotoxicity mechanistic studies.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"21 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Portable proton transfer reaction ion trap mass spectrometer with selectable buffer gas. 便携式质子转移反应离子阱质谱仪，可选择缓冲气体。

IF 4.2 3区化学

Analyst Pub Date : 2025-09-29 DOI: 10.1039/d5an00703h

Jun Wang,La Chen,Chengjie Jiang,Gangqiang Li,Luhong Wen

{"title":"Portable proton transfer reaction ion trap mass spectrometer with selectable buffer gas.","authors":"Jun Wang,La Chen,Chengjie Jiang,Gangqiang Li,Luhong Wen","doi":"10.1039/d5an00703h","DOIUrl":"https://doi.org/10.1039/d5an00703h","url":null,"abstract":"Proton transfer reaction mass spectrometry (PTR-MS) is a rapid and highly sensitive technique for the detection of volatile organic compounds and has been increasingly used in the fields of environmental monitoring, medicinal diagnosis and food industry. The large size and heavy weight of conventional PTR-MS devices limit their on-site application for real-time and rapid analysis. In this work, a miniaturized PTR-MS instrument with a total weight of 25 kg was developed on the basis of a portable ion trap mass spectrometer, which was characterized by the use of a small hollow-cathode discharge for hydroxonium ion generation and a compact ion funnel as the drift tube. For this instrument, mass resolution, ion isolation and fragmentation efficiencies, and sensitivity have been investigated in detail with helium and nitrogen as buffer gases. Compared with nitrogen buffer gas, when helium buffer gas was used, the mass resolution of protonated acetone ions doubled, whereas the ion fragmentation efficiency could be increased by a factor of 17. With helium buffer gas, the limits of detection for acetone, benzene, and toluene were found to be 1 ppbv, 0.5 ppbv, and 0.1 ppbv, respectively, and linear calibration curves for these compounds were established across concentration ranges spanning at least two orders. Under the optimal vacuum pressures of 1.5 mbar and 2.5 mbar, the gas consumption rates for helium and nitrogen were 81 mL min-1 and 60 mL min-1, respectively. In addition, the sample injection flow rate exerted a pronounced influence on the detection sensitivity and response time of the system.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"3 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0