Analyst最新文献 - Book学术

A novel double-stranded probe minimizes false-positive and false-negative LAMP results with ultrasensitivity and high specificity 一种新型双链探针以超灵敏度和高特异性最大限度地减少假阳性和假阴性LAMP结果

IF 4.2 3区化学

Analyst Pub Date : 2026-10-20 DOI: 10.1039/d6an00881j

Jie Qu,Lixia Mei,Xinxin Li,Hong Zhang,Hongfei He,Yuan Xu,Yongjun Yang,Guangcheng Luo

{"title":"A novel double-stranded probe minimizes false-positive and false-negative LAMP results with ultrasensitivity and high specificity","authors":"Jie Qu,Lixia Mei,Xinxin Li,Hong Zhang,Hongfei He,Yuan Xu,Yongjun Yang,Guangcheng Luo","doi":"10.1039/d6an00881j","DOIUrl":"https://doi.org/10.1039/d6an00881j","url":null,"abstract":"Loop-mediated isothermal amplification (LAMP) is one of the most widely used molecular diagnostic methods and is considered a promising tool for point-of-care testing (POCT). However, because LAMP requires multiple complex primers, false-positive and false-negative results can occur, thereby limiting its clinical applicability. Herein, we propose a novel chemically modified double-stranded probe (CDP) to reduce false-positive and false-negative LAMP results, thereby developing CDP-assisted LAMP (CLAMP). The CDP consists of a recognition strand (RS) and a 2′-fluoro (2′-F)-modified signal strand (SS). We found that the 2′-F-modified SS has a significantly higher melting temperature than its natural counterpart, providing excellent thermostability and a high signal-to-noise ratio. During the CLAMP reaction, the CDP specifically binds to LAMP amplicons via its RS, and the SS is efficiently displaced by Bst DNA polymerase, enabling specific fluorescence generation. With the assistance of CDP, CLAMP effectively reduces false-positive and false-negative results and tolerates common interfering compounds. Furthermore, CLAMP was 100-fold more sensitive than conventional LAMP and exhibited 100% accuracy in clinical validation. In summary, a novel CDP-based strategy was established to enhance the performance of LAMP, thereby improving its applicability and offering a promising tool for POCT molecular diagnosis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"13 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A tape-based intestinal chip co-culture model for assessing anti-inflammatory activity of chitosan oligosaccharides 基于胶带的肠道芯片共培养模型评估低聚糖的抗炎活性

IF 4.2 3区化学

Analyst Pub Date : 2026-10-20 DOI: 10.1039/d6an00749j

Jiayi Yan,Xiuli Wu,Jingyan Gao,Wentao Su,Xiaomeng Ren,Fanhua Kong,Shuang Song

{"title":"A tape-based intestinal chip co-culture model for assessing anti-inflammatory activity of chitosan oligosaccharides","authors":"Jiayi Yan,Xiuli Wu,Jingyan Gao,Wentao Su,Xiaomeng Ren,Fanhua Kong,Shuang Song","doi":"10.1039/d6an00749j","DOIUrl":"https://doi.org/10.1039/d6an00749j","url":null,"abstract":"Intestinal inflammation is a central pathological process in gastrointestinal diseases, yet conventional in vitro models poorly reproduce epithelial–immune interactions, and accessible platforms for rapid anti-inflammatory screening remain lacking. Here, a low-cost, high-throughput tape-based intestinal chip co-culturing Caco-2 epithelial cells and RAW264.7 macrophages under dynamic flow was applied to approximate the epithelial–macrophage interface of the intestinal wall and to evaluate the anti-inflammatory activity of chitosan oligosaccharides (COS). Three COS fractions (COS1, COS2, COS3; 3.6, 2.7, and 0.8 kDa) prepared from Antarctic krill shell exhibited molecular weight-dependent transepithelial permeability, with COS3 showing the highest permeability. All COS fractions significantly suppressed LPS-induced NF-κB p65 nuclear translocation, macrophage morphological activation, and the production of NO, IL-1β, TNF-α, and IL-6, with the low-molecular-weight COS3 showing the strongest effects under equal-mass dosing. Targeted metabolomics identified four candidate marker metabolites (proline, asparagine, GTP, and α-ketoglutaric acid) that were elevated by LPS and partially restored by COS treatment, providing a metabolic signature for rapid evaluation of anti-inflammatory efficacy. These findings establish the integrated tape-based chip–metabolomics strategy as an accessible platform for screening anti-inflammatory bioactive compounds and highlight the potential of low-molecular-weight COS as anti-inflammatory agents.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"33 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Sensitive and Visual Colorimetric Biosensor for Liver Cancer‑Associated miRNA Using Exo‑III Assisted Target Recycling and a Cyclization‑Functional DNAzyme Walker 使用Exo - III辅助靶循环和环化功能DNAzyme Walker的肝癌相关miRNA的灵敏和视觉比色生物传感器

IF 4.2 3区化学

Analyst Pub Date : 2026-10-20 DOI: 10.1039/d6an01082b

Guanchao Zhang,Lei Jia,Bing Xu

{"title":"A Sensitive and Visual Colorimetric Biosensor for Liver Cancer‑Associated miRNA Using Exo‑III Assisted Target Recycling and a Cyclization‑Functional DNAzyme Walker","authors":"Guanchao Zhang,Lei Jia,Bing Xu","doi":"10.1039/d6an01082b","DOIUrl":"https://doi.org/10.1039/d6an01082b","url":null,"abstract":"The accurate detection of liver cancer-associated microRNAs (miRNAs) remains challenging due to their low abundance and high sequence homology. Herein, we report a colorimetric biosensor integrating Exo-III-assisted target recycling with a cyclization-functional DNAzyme walker for sensitive and highly specific miRNA detection. Notably, the assay incorporates two distinct miRNA recognition events: first, the target miRNA hybridizes with a stem-loop probe to trigger Exo-III-mediated cyclic cleavage; second, the released initiator strand activates the DNA walker on magnetic beads, where the miRNA must hybridize with a specific binding site on the tracker strand to reconstitute the split DNAzyme catalytic core. This dual-recognition mechanism dramatically enhances specificity, enabling the sensor to discriminate single- and double-nucleotide mismatches with high reliability. Coupled with Ag⁺-aptamer-mediated signal transduction, the platform produces a visible color change for naked-eye readout. With cascade amplification, the assay achieves a detection limit of 2.7 fM and a linear range of 5 fM to 100 pM. Successful spike-recovery tests in human serum (96.2–104.4%) suggest its potential for clinical application. This portable, visual colorimetric strategy offers a promising solution for early liver cancer screening.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"12 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensors for human and planetary health 用于人类和地球健康的传感器

IF 4.2 3区化学

Analyst Pub Date : 2026-10-20 DOI: 10.1039/d6an90064j

Priyanka Dey,Klaus Koren,Baohong Liu,Rebecca Whelan

引用次数: 0

A one‑step LAMP‑CRISPR/Cas12a Platform Targeting lytA and cpsA for Rapid Parallel Identification and Virulence Stratification of Streptococcus pneumoniae 一种针对lytA和cpsA的LAMP - CRISPR/Cas12a平台用于肺炎链球菌快速平行鉴定和毒力分层

IF 4.2 3区化学

Analyst Pub Date : 2026-10-20 DOI: 10.1039/d6an01151a

Wanying Luo,Linping Fan,Dan Dan WEI,Chunxiang Xiang,Hengyi Xu,Yang Liu,Wei Zhang

{"title":"A one‑step LAMP‑CRISPR/Cas12a Platform Targeting lytA and cpsA for Rapid Parallel Identification and Virulence Stratification of Streptococcus pneumoniae","authors":"Wanying Luo,Linping Fan,Dan Dan WEI,Chunxiang Xiang,Hengyi Xu,Yang Liu,Wei Zhang","doi":"10.1039/d6an01151a","DOIUrl":"https://doi.org/10.1039/d6an01151a","url":null,"abstract":"Streptococcus pneumoniae (S. pneumoniae, SPN) is the primary pathogen causing community-acquired pneumonia (CAP), purulent meningitis, sepsis, and acute otitis media. Accurate species identification and rapid differentiation of invasive strains are critical for clinical management and infection control. Based on loop-mediated isothermal amplification (LAMP) and CRISPR/Cas12a technologies, this study established a parallel dual‑target detection platform targeting the S. pneumoniae-specific lytA gene and the capsular polysaccharide synthesis regulatory gene cpsA. A dual‑readout mode combining fluorescence detection (FD) and lateral flow test strip (LFTS) was adopted to meet diverse laboratory testing requirements. For each target‑specific reaction, LAMP amplification and CRISPR/Cas12a cleavage were sequentially performed in a single closed tube using paraffin‑oil stratification to suppress solvent evaporation, effectively preventing aerosol contamination. Range‑finding serial‑dilution assays revealed that the lowest gDNA concentrations producing positive signals in all three technical replicates reached 1 fg μL⁻¹ (FD) and 10 fg μL⁻¹ (LFTS). This assay specifically distinguished S. pneumoniae from other common clinical pathogens and preliminarily identified encapsulated invasive strains, with 100% sensitivity and specificity consistent with conventional PCR. This LAMP-CRISPR/Cas12a platform, with its closed-tube design, avoids contamination, while FD and LFTS dual readouts cater to diverse testing needs. Featuring high sensitivity, excellent specificity, simplicity, and speed, it provides an innovative and practical solution for the rapid diagnosis of S. pneumoniae and holds great clinical potential.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"33 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On-site rapid screening of ethylene etonitazene via a versatile conjugated-polymer fluorescence platform 通过多功能共轭聚合物荧光平台现场快速筛选乙烯对硝基甲苯

IF 4.2 3区化学

Analyst Pub Date : 2026-10-19 DOI: 10.1039/d6an00886k

Xuemeng Tian,Ying Jiao,Chunqiang Liu,Yafei Song,Sameer Hussain,Yi Hao,Sami Alharbi,Yue Wang,Qi Liao,Ruixia Gao

引用次数: 0

A multi-modal mass spectrometric approach for lipid analysis enabled by online fractionation of complex mixtures in a solid-phase extraction cartridge. 一种多模态质谱方法，用于脂质分析，可在固相萃取筒中在线分馏复杂混合物。

IF 3.6 3区化学

Analyst Pub Date : 2026-10-19 DOI: 10.1039/d6an00926c

Octavion Spears, Alexander Grooms, Benjamin Burris, Abraham K Badu-Tawiah

{"title":"A multi-modal mass spectrometric approach for lipid analysis enabled by online fractionation of complex mixtures in a solid-phase extraction cartridge.","authors":"Octavion Spears, Alexander Grooms, Benjamin Burris, Abraham K Badu-Tawiah","doi":"10.1039/d6an00926c","DOIUrl":"https://doi.org/10.1039/d6an00926c","url":null,"abstract":"This study aimed to develop a direct infusion analytical platform for analyzing complex lipid mixtures using a solid-phase extraction (SPE) cartridge. The SPE cartridge was developed in-house and coupled to a contained-electrospray ionization (cESI) source, which enabled online fractionation of lipids followed by real-time mass spectrometry (MS) analysis of eluates. The SPE cartridge was packed with a silica sorbent. We used multiple solvents with varying polarities to sequentially elute and characterize different lipids. To stabilize the electrospray signal derived from nonpolar organic solvents, our SPE-cES-MS platform utilized a co-axial spray system that allowed an ammonium acetate containing lipase enzyme to be delivered from a second electrospray capillary. In its fullest implementation, the SPE-cESI-MS platform was applied to fractionate a sample mixture containing 18 different lipids using hexane, ethanol, and water for sequential elution from the silica sorbent. Together, the three elution steps enabled 93.0% of the lipids in the mixture to be detected, with overall improvement in signal intensities. In contrast, without the miniaturized SPE front-end, direct analysis of the 18-component lipid mixture resulted in the detection of 85.7% of the lipids. The solvents employed were optimized by evaluating their selectivity for polar and nonpolar lipids. The limits of detection associated with the elution of nonpolar lipids (e.g., trilinolein) from the polar silica surface were determined to be 0.62 µM and 0.84 µM for the elution of polar oleic acid from the same sorbent. This work represents a rapid and affordable analytical approach for analyzing complex lipid mixtures in situations where conventional chromatography cannot be used, including analysis on a portable mass spectrometer.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing non-covalent membrane protein-lipid interactions using sheath liquid capillary electrophoresis-native top-down mass spectrometry. 利用鞘液毛细管电泳-天然自上而下质谱法探测非共价膜蛋白-脂质相互作用。

IF 3.6 3区化学

Analyst Pub Date : 2026-10-16 DOI: 10.1039/d6an00941g

Kanchan Pathak, Delaynie Brinkman, Sara Walters, Gustavo Perrotti, Brian Fuglestad, Fabio P Gomes

引用次数: 0

Surface reduction-boosted fluorescence of carbon dots for highly sensitive alkaline phosphatase sensing. 高灵敏度碱性磷酸酶检测用碳点表面还原增强荧光。

IF 3.6 3区化学

Analyst Pub Date : 2026-10-16 DOI: 10.1039/d6an00766j

Xiliang Yang, Suyi Wu, Yunjuan Zhang, Mingfeng Li, Xin Huang, Cui Liu, Chuan-Hua Zhou

引用次数: 0

Contact lens microplastics: rapid Py-TOFMS quantification and ocular health risk assessment 隐形眼镜微塑料：Py-TOFMS快速定量及眼健康风险评估

IF 4.2 3区化学

Analyst Pub Date : 2026-10-15 DOI: 10.1039/d6an00755d

Jingwen Zhang,Haodong Gao,Jiangle Zhang,Yidan Tang,Jiahui Wang,Yu Liu,Hanli Xuan,Hongyao Wang,Ying Zeng

{"title":"Contact lens microplastics: rapid Py-TOFMS quantification and ocular health risk assessment","authors":"Jingwen Zhang,Haodong Gao,Jiangle Zhang,Yidan Tang,Jiahui Wang,Yu Liu,Hanli Xuan,Hongyao Wang,Ying Zeng","doi":"10.1039/d6an00755d","DOIUrl":"https://doi.org/10.1039/d6an00755d","url":null,"abstract":"Microplastics (MPs) released from contact lenses during wear can persistently contact the ocular surface through tear circulation, thereby posing a threat to ocular health. Combining thermal analysis with mass spectrometry enables accurate quantitative detection of low-concentration MPs from contact lenses, supporting exposure risk assessment. In this study, a hyphenated device consisting of a mini-pyrolyzer and a time-of-flight mass spectrometer (Py-TOFMS) was developed to enable rapid quantitative detection of MPs released from contact lenses. Compared with conventional Py-GC/MS, which typically requires approximately 30 min per sample, the proposed Py-TOFMS method eliminates the GC separation step and completes the analysis in 15 min, thereby simplifying the detection workflow and reducing the risk of sample loss and contamination. Py-TOFMS exhibited good limit of detection (LOD = 0.09 μg) and limit of quantification (LOQ = 0.27 μg), along with good linearity (R2 ≥ 0.98). This study systematically simulated daily use conditions, including soaking, heating, and ultraviolet (UV) radiation. All tested contact lenses were found to release MPs, with the most significant release occurring under UV exposure. Furthermore, by combining optical microscopy and infrared spectroscopy to observe the morphological distribution of MPs and the aging effect of contact lenses, a preliminary systematic assessment was conducted on the potential ocular health risks associated with the release of MPs from contact lenses. This study reveals that UV exposure poses the most significant risk of MPs release from contact lenses, underscoring the urgency of assessing the potential health risks associated with ocular exposure to MPs.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"6 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149173177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0