Analyst最新文献

{"title":"Spatiotemporal 3D cell impedance monitoring for metal nanoparticle risk assessment by plug-in vertical electrode array†","authors":"Yimin Shi, Hui Liu, Mingda Zhao, Sheng Sun, Meiyan Qin, Yang Zhao, Mingxiao Li, Lina Zhang, Lingqian Zhang and Chengjun Huang","doi":"10.1039/D4AN01494D","DOIUrl":"10.1039/D4AN01494D","url":null,"abstract":"<p >Metal nanoparticles are commonly found in our daily lives and pose great risks to people's health. Therefore, it is crucial to establish a research model for the toxic effects of metal nanoparticles. In recent decades, three-dimensional (3D) cell models have attracted increasing interest in the fields of cell barriers, nanotoxicology, and drug screening, as they have significant advantages over two-dimensional (2D) cell models in accurately simulating <em>in vivo</em> behavior of human cells. The accurate spatiotemporal reaction characteristics achieved through the diffusion effect of metal nanoparticles in Matrigel scaffolds are of great importance in nanotoxicology. However, traditional impedance sensors face challenges in performing spatiotemporal dynamic impedance monitoring and evaluating the toxic impact of metal nanoparticles on 3D cells. Here, we propose an impedance sensor that integrates a plug-in vertical electrode array (PVEA) chip with a multi-channel detection system. This sensor can dynamically record 3D cell impedance in the vertical direction, which is consistent with the temporal and spatial progression of metal nanoparticle penetration, and also closely related to the spatiotemporal activity of cells influenced by metal nanoparticles. This method can detect subtle changes in impedance signals at different positions caused by the diffusion of metal nanoparticles, and has high application value in Nanotoxicology evaluation. This universal, high-throughput 3D cell impedance sensor has great potential in toxicity detection and drug screening.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 6","pages":" 1081-1090"},"PeriodicalIF":3.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A water-soluble glucose-appended quinoline–benzothiazole conjugate as a selective and sensitive receptor for Cu+ ions in aqueous media and intracellular bio-imaging in live cells†","authors":"Ravinkumar Valand, Nidhi Pandey, Jayesh Bellare and Areti Sivaiah","doi":"10.1039/D5AN00066A","DOIUrl":"10.1039/D5AN00066A","url":null,"abstract":"<p >A water-soluble and biologically benign glucose-appended quinoline–benzothiazole conjugate (<strong>L</strong>) has been synthesized and characterized using various spectroscopy techniques. The recognition properties of <strong>L</strong> show selective recognition of Cu<small>⁺</small> ions among other biological metal ions including Cu<small>²⁺</small> ions in PBS buffer at pH 7.4. <strong>L</strong> exhibited a switch-on fluorescence enhancement upon the addition of Cu<small>⁺</small> with a detection limit of 1.48 × 10<small>⁻⁸</small> M in an aqueous medium. Job's plot confirmed the 1 : 1 binding ratio observed between probe <strong>L</strong> and Cu<small>⁺</small> ions with an association constant (<em>K</em><small>_a</small>) of 1.46 × 10<small>⁵</small> M<small>⁻¹</small>. The proposed complex binding mechanism was supported by UV-Vis, fluorescence and ESI-MS. The coordination features of the {<strong>L</strong> + Cu<small>⁺</small>} complex were delineated using DFT computational calculations. Furthermore, <strong>L</strong> was successfully applied for intracellular fluorescence bio-imaging of Cu<small>⁺</small> ions in L929 living cells, suggesting that it holds significant potential for Cu<small>⁺</small> ion bio-imaging and disease diagnosis.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 6","pages":" 1176-1186"},"PeriodicalIF":3.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Present and future of smartphone-coupled chemiluminescence and electrochemiluminescence assays: a mini-review","authors":"Chunuranjan Dutta, Daniel Citterio and Pabitra Nath","doi":"10.1039/D4AN01438C","DOIUrl":"10.1039/D4AN01438C","url":null,"abstract":"<p >The convergence of smartphones with chemiluminescence and electrochemiluminescence (CL/ECL) assays marks a transformative leap in the realm of sensing technologies. The traditional CL/ECL assays, known for their high sensitivity and versatility, find extensive applications in medical diagnostics, environmental monitoring, food safety, and forensic sciences. However, these techniques have long been constrained due to the requirement of expensive instrumentation and complex reagent handling and hence their accessibility within certain environments is limited. In an era where rapid, accurate, and routine analysis is critical, smartphone-enabled CL/ECL systems offer substantial advantages over conventional analytical methods. By leveraging the universal accessibility and technological sophistication of smartphones and combining them with CL/ECL-based sensing, the smartphone has evolved into a cost-efficient and accessible analytical platform. The ability of the combined platform to conduct on-site analysis in real-time with minimal effort has emerged as a game-changer, particularly in low-resource settings. This mini-review explores the rapid evolution of smartphone-coupled CL/ECL systems over the last five years. The article covers the areas where the combined platform has been implemented in recent years for various sensing applications. The review further identifies key challenges that are associated with such combined platforms and finally highlights the future perspectives of the present topic.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 6","pages":" 1033-1047"},"PeriodicalIF":3.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and mechanism analysis of an ultra-sensitive GCN/Pb2+/PPy heterojunction electrochemiluminescent sensor for detecting Cu(ii)†","authors":"Jie Wang, Yuhui Yi, Mu Yang, Dongyan Tian, Jinshan Liu, Yu Yu, Songmei Wu and Kejian Ding","doi":"10.1039/D4AN01564A","DOIUrl":"10.1039/D4AN01564A","url":null,"abstract":"<p >A highly stable electrochemiluminescent (ECL) sensor based on graphitic carbon nitride (GCN)/Pb<small>²⁺</small>/polypyrrole (PPy) was developed to detect trace copper ions. The hydrochloric acid immersion technique was applied to rearrange PPy chains for the first time. Under optimal experimental conditions, the ECL intensity increased linearly with the increase of Cu<small>²⁺</small> concentration in solution from 10<small>⁻¹²</small> M to 10<small>⁻⁶</small> M (1 pM to 1 μM), with a super low limit of detection (LOD) of 10<small>⁻¹²</small> M (1 pM) (S/N = 3). Additionally, the impact of detection on the localized states of the composite nanomaterial was revealed by applying Vienna <em>ab initio</em> Simulation Package (VASP) and Gaussian 16.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 6","pages":" 1112-1121"},"PeriodicalIF":3.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A low-cost, high-sensitivity 3D printed fluorescence detector","authors":"Robyn A Snow, Paul Steven Simone Jr., Gary Emmert, Michael A Brown","doi":"10.1039/d4an01378f","DOIUrl":"https://doi.org/10.1039/d4an01378f","url":null,"abstract":"Fluorescence methods have distinct advantages over traditional absorbance methods including greater sensitivity, improved detection limits, and selectivity. Unfortunately, the cost of typical, commercially available fluorescence detectors is beyond what some industrial and research labs can afford or maintain. Having a relatively low-cost, simple to use, and high-sensitivity fluorescence detector would be very beneficial. The aim of this research is to develop a 3D printed flow through fluorescence detector that does not require complex optics or an expensive excitation source and has comparable performance to a commercial detector. The detector presented here was designed to work with nicotinamide-based methods developed in previous research; however, by simply changing the excitation and emission filters this detector can be adapted to other applications. Several evaluation studies were performed where the relative signal-to-noise ratio, detection limits, accuracy, and precision results for the 3D printed detector were compared to those of a commercial detector using nicotinamide as the analyte. Overall, the detector performed comparably or better than a commercial detector for these metrics.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"76 2 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An electrochemical biosensor utilizing CRISPR/Cas12a amplification for the detection of E. coli†","authors":"Chenyan Li, Yilan Liang and Qincong Feng","doi":"10.1039/D4AN01441C","DOIUrl":"10.1039/D4AN01441C","url":null,"abstract":"<p >Electrochemical biosensors are frequently employed to identify harmful microbes and disease indicators. However, their practical applicability is constrained by poor signal amplification efficiency and immobilization processes on the probe surface. To get over these restrictions, we here integrated roll-cycling amplification (RCA) and CRISPR/Cas12a gene editing tools with electrochemical biosensors. We constructed an electrochemical biosensor based on RCA to activate the cleavage activity of Cas12a. First, by double-stranded nucleic acid aptamer (ds Apt) specifically binding to <em>E. coli-2571</em>, Apt-b was competitively isolated to bind to T4 ligase and produce circular DNA. This in turn activates RCA, which in turn activates the accessory cleavage activity of CRISPR/Cas12a, which in turn cleaves DNA sequences loaded onto the electrode, changing electrochemical signals. With a linear range of 1 × 10<small>²</small>–1 × 10<small>⁷</small> CFU mL<small>⁻¹</small>, a detection limit of 5.28 CFU mL<small>⁻¹</small>, and experimental recoveries of 93.01–101.53%, the measured electrochemical signals were positively connected with the concentration of <em>E. coli-2571</em> under the optimal experimental conditions. Therefore, by combining two approaches—RCA and CRISPR/Cas12a—our electrochemical biosensor was able to detect <em>E. coli-2571</em> specifically and sensitively, providing new research opportunities for the detection of other harmful bacteria.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 6","pages":" 1158-1166"},"PeriodicalIF":3.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive view of the molecular features within the serum and serum EV of Alzheimer's disease†","authors":"Jiayi Zhang, Xiaoqin Cheng, Anqi Hu, Xin Zhang, Meng Zhang, Lei Zhang, Jiawei Dai, Guoquan Yan, Huali Shen and Guoqiang Fei","doi":"10.1039/D4AN01018C","DOIUrl":"10.1039/D4AN01018C","url":null,"abstract":"<p >Conventional Alzheimer's disease research mainly focuses on cerebrospinal fluid, which requires an invasive sampling procedure. This method carries inherent risks for patients and could potentially lower patient compliance. EVs (Extracellular Vesicles) and blood are two emerging noninvasive mediators reflecting the pathological changes of Alzheimer's disease. Integrating the two serum proteomic information based on DIA (Data Independent Acquisition) is conducive to the comparison of serological research strategies, mining pathological information of AD, and evaluating the potential of EVs and blood in the diagnosis of AD. We generated a combined proteomic data resource of 39 serum samples derived from patients with AD and from age-matched controls (AMC) and identified 639 PGs (protein groups) in serum samples and 714 PGs in serum EV samples. The differentially expressed protein groups identified in both serum and serum EV provide a reflective profile of the pathological characteristics associated with AD. The combined strategy performed well, identifying 40 potential diagnostic markers with AUC values above 0.85, including two molecular diagnostic models that achieved an effectiveness score of 0.991.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 5","pages":" 922-935"},"PeriodicalIF":3.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic enhancement of the Ag/ZIF-67 cage@MXene 3D heterogeneous structure for ultrahigh SERS sensitivity and stability†","authors":"Yunpeng Shao, Wenlong Deng, Yue Niu, Zicheng Zhang, Jiwei Song, Yuan Yao and Linyu Mei","doi":"10.1039/D4AN01493F","DOIUrl":"10.1039/D4AN01493F","url":null,"abstract":"<p >There is an urgent need in the <em>in situ</em> field for rapid extraction and analysis of target molecules from irregular surfaces. The application of SERS technology is often limited by low adhesion between precious metal nanoparticles and the substrate and complex fabrication processes. In order to solve this problem, a carbon fiber cloth (CFC) loaded Ag/ZIF-67 cage@MXene 3D detection platform (AZMC) was constructed in this study. The platform takes advantage of the large surface area and defects of MXene flakes to host noble metals, the high carrier transport efficiency between flakes, and van der Waals forces to build highly sensitive and stable composite SERS substrates. The hydrophilicity and subsurface oxidation behavior of MXene make its optoelectronic performance unstable. In this study, the ZIF-67 cage was chemically bonded to MXene through the Co–O–Ti bond, and the ZIF 67@MXene heterojunction was successfully constructed to maintain the optimal photoelectric stability and excellence of MXene. The high performance of the substrate stems from the synergistic effects of charge transfer (CT) and surface plasmon resonance (SPR) of AgNPs and MXene flakes, while the 3D nanocage structure provides additional hotspot regions. Substrate sensitivity was analyzed using rhodamine 6G (R6G) as a probe molecule (detection limit as low as 10<small>⁻¹¹</small> M). Notably, the AZMC substrate is highly stable (SERS performance remains essentially unchanged after 45 days of exposure to air). Using this substrate, we also successfully analyzed methylene blue (MB) molecules and Sudan I molecules on apple epidermis, which were successfully detected at concentrations of 0.5 mg L<small>⁻¹</small> and 1 mg L<small>⁻¹</small>, respectively.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 6","pages":" 1131-1139"},"PeriodicalIF":3.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A non-enzymatic highly stable electrochemical sensing platform based on allylamine capped copper nanoparticles for the detection of the soil nitrate content†","authors":"Bimalendu Mukherjee, Mukti Mandal, Raghavv Raghavender Suresh, Shantanu Kar, Binaya Kumar Parida, Somsubhra Chakraborty and Gorachand Dutta","doi":"10.1039/D4AN01345J","DOIUrl":"10.1039/D4AN01345J","url":null,"abstract":"<p >Nitrate (NO<small>₃</small><small>⁻</small>) ion contamination of water is a major issue that affects many parts of the world due to excessive usage of nitrogen containing fertilizers in the soil. Hence, quantification of NO<small>₃</small><small>⁻</small> ions in the soil is of utmost importance. In the present research work, we have developed an efficient and highly stable non-enzymatic electrochemical sensor for NO<small>₃</small><small>⁻</small> ion detection based on allylamine capped copper nanoparticles (Alym@CuNPs) decorated on exfoliated multi-walled carbon nanotubes (Exf-CNTs). Herein, we have addressed the sensor surface storage stability issue of copper nanoparticle based electrochemical sensors for the first time and confirmed the superior storage stability of the Alym@CuNPs modified glassy carbon electrode (GCE) over uncapped copper nanoparticles (uCuNPs) and electrodeposited copper nanoparticles (eCuNPs) modified GCEs. In comparison to the bare GCE, Exf-CNT/GCE and Alym@CuNPs/Exf-CNT/GCE, the proposed Alym@CuNPs-Nafion (NF)/Exf-CNT/GCE demonstrated enhanced catalytic activity towards the electro-reduction of NO<small>₃</small><small>⁻</small> ions (pH = 2) under optimal experimental conditions, with a considerable increase in cathodic peak currents. Along with that, no inert gas was purged into the electrolyte medium prior to the detection of NO<small>₃</small><small>⁻</small> ions which makes the sensor more reliable under real environmental conditions. The sensor displayed broad linear ranges from 10 to 1000 μM (<em>R</em><small>²</small> = 0.997), with a limit of detection (LOD) of 5.28 μM (<em>n</em> = 3) for NO<small>₃</small><small>⁻</small> ion detection. The sensor surface shows excellent storage stability even up to 45 days with 97.8% retention in current value which is much higher compared to the previously reported works. Additionally, the sensor surface shows promising reproducibility and repeatability results with RSD values of 1.78% and 0.91%, respectively. Moreover, the proposed sensor is successfully utilized to detect NO<small>₃</small><small>⁻</small> ions in real soil samples.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 5","pages":" 936-952"},"PeriodicalIF":3.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-time detection of organophosphorus pesticides in water using an unmanned boat-borne Raman spectrometer†","authors":"Chen Zhang, Jiahui Zhu, Wenqi Ye, Xiaohong Liu, Ruishuang Zhuo, Chi Wang, Hong Liu and Wei Zhang","doi":"10.1039/D4AN01587H","DOIUrl":"10.1039/D4AN01587H","url":null,"abstract":"<p >Organophosphorus pesticides (OPs) have been widely used in agricultural production. However, their potential harm to human health and the environment cannot be ignored. On-site detection of OPs in water is valuable for pollution source tracing and early warning of environmental pollution. We design an unmanned boat detection system equipped with a Raman spectrometer, which can achieve automated sampling and real-time detection of OPs in water. The unmanned boat detection system integrates automatic sampling, chemical reaction, spectral detection, and signal transmission modules. This method can specifically detect OPs in water by utilizing the inhibition effect of OPs on acetylcholinesterase activity. The minimum detection concentration is 700 nM for parathion-methyl. This method shows good anti-interference ability for other kinds of pesticides, demonstrating its applicability for rapid detection of OPs under complex water environmental conditions. Our strategy provides an efficient solution for real-time and flexible monitoring of OPs in water environments.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 6","pages":" 1122-1130"},"PeriodicalIF":3.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}