Analyst最新文献_第10页

An innovative electrochemiluminescent immunosensor using dual amplified signals from AuNPs@CoSn(OH)6 for the detection of the AD biomarker: amyloid beta 1–40† 利用 AuNPs@CoSn(OH)6的双重放大信号检测 AD 生物标记物：淀粉样蛋白-β 1-40 的创新型电化学发光免疫传感器

IF 3.6 3区化学

Analyst Pub Date : 2025-04-23 DOI: 10.1039/D5AN00048C

Jiaojing Sun, Wenqing Geng, Yueju Wang, Huiling Li, Rong Tan and Yifeng Tu

{"title":"An innovative electrochemiluminescent immunosensor using dual amplified signals from AuNPs@CoSn(OH)6 for the detection of the AD biomarker: amyloid beta 1–40†","authors":"Jiaojing Sun, Wenqing Geng, Yueju Wang, Huiling Li, Rong Tan and Yifeng Tu","doi":"10.1039/D5AN00048C","DOIUrl":"10.1039/D5AN00048C","url":null,"abstract":"Alzheimer's disease (AD) is a degenerative condition of the nervous system that causes severe damage to patients’ daily activities and quality of life. Amyloid beta 1–40 protein (Aβ40), which is involved in the formation of cerebral plaques, is one of the crucial biomarkers related to AD. Herein, a novel and highly sensitive immunosensor for the detection of Aβ40 is developed. Using a reinforced indium tin oxide-coated glass with a nanocomposite of gold nanoparticle-enhanced CoSn(OH)6 (AuNPs@CoSn(OH)6) to trigger the electrochemiluminescence (ECL) of luminol as the sensing signal, the immunosensor is fabricated by immobilizing the Aβ40 antibody onto it. By integrating the high immune specificity, excellent conductivity and catalytic activity of the nanocomposite, the resultant immunosensor can be successfully employed to detect the target in real samples. The formation of the immune complex leads to increased steric hindrance and electron transfer resistance, which in turn causes a declined ECL output when the target Aβ40 binds to the antibody on the sensor surface. Under optimized conditions, the developed ECL immunosensor exhibits a linear response for Aβ40 ranging from 1 to 800 pg mL−1 and a low detection limit of 0.47 pg mL−1. Experimentally, it is demonstrated to be highly sensitive, specific, reproducible and stable. This work extends the application of the perovskite CoSn(OH)6 and AuNPs in the field of ECL immunosensing and provides a novel strategy for clinical research on Alzheimer's disease.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 11","pages":" 2259-2269"},"PeriodicalIF":3.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Headspace separation–combined fluorescence strategy for highly selective detection of hydrogen sulfide using silver nanocluster assemblies as a probe via a self-made device† 利用自制装置，以银纳米团簇为探针，顶空分离结合荧光策略进行高选择性硫化氢检测

IF 3.6 3区化学

Analyst Pub Date : 2025-04-22 DOI: 10.1039/D5AN00220F

Ying Zhu, Ran Li, Zhongshuai Zhao and Zhongde Liu

{"title":"Headspace separation–combined fluorescence strategy for highly selective detection of hydrogen sulfide using silver nanocluster assemblies as a probe via a self-made device†","authors":"Ying Zhu, Ran Li, Zhongshuai Zhao and Zhongde Liu","doi":"10.1039/D5AN00220F","DOIUrl":"10.1039/D5AN00220F","url":null,"abstract":"Hydrogen sulfide (H2S) is a poisonous gas and endogenously produced signaling molecule in mammalian systems, and its highly selective and rapid monitoring is urgently desired. Nevertheless, the performance of nearly all the fluorescence probes for H2S detection is limited by interferences caused by other non-volatile thiol-containing species. To address this issue, herein, a headspace separation–combined fluorescence strategy was attempted by utilizing thiosalicylic acid (TSA)-capped silver nanoclusters (AgNCs@TSA) as probes. AgNCs@TSA showed a significant aggregation-induced emission (AIE) phenomenon; Moreover, upon the introduction of H2S, the responsive disassembly of its aggregates occurred due to the competitive Ag–sulfur bond formation between H2S and TSA molecules on the surface of the AgNCs, accompanied by a distinctive luminescence quenching. Using hybrid analysis, superior selectivity could be obtained, and the signal change (ΔI) of AgNCs@TSA showed good linearity at an H2S concentration of 0.1–100 μM with a detection limit (3σ) of 72.2 nM. In monitoring sulfide levels in real samples, the approach yielded recoveries from 98.3% to 103.7% with a relative standard deviation (RSD) of less than 3.1%. The approach described here may be readily extended to the analysis of other volatile components, with the advantage of easy elimination of interferences. Furthermore, the hybrid optical strategy is expected to be a flexible and versatile platform for the on-site detection of volatile components.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 11","pages":" 2336-2342"},"PeriodicalIF":3.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocompatible AgInS2@hydrogel microelectrode with enhanced photoelectrochemical sensitivity for real-time in vivo dopamine monitoring† 生物相容性AgInS2@hydrogel微电极与增强的光电化学灵敏度用于实时体内多巴胺监测†

IF 3.6 3区化学

Analyst Pub Date : 2025-04-22 DOI: 10.1039/D5AN00372E

Shiting Gu and Zhonghai Zhang

{"title":"Biocompatible AgInS2@hydrogel microelectrode with enhanced photoelectrochemical sensitivity for real-time in vivo dopamine monitoring†","authors":"Shiting Gu and Zhonghai Zhang","doi":"10.1039/D5AN00372E","DOIUrl":"10.1039/D5AN00372E","url":null,"abstract":"In situ monitoring of neurochemical dynamics is pivotal for understanding brain function and diagnosing neurological disorders. Conventional photoelectrochemical (PEC) sensors face limitations due to poor tissue compatibility and insufficient light penetration depth in vivo. Herein, we present a transparent and conductive hydrogel-based microelectrode (AgInS2@hydrogel) that integrates a biocompatible topological hydrogel with an AgInS2 semiconductor for selective dopamine (DA) detection. The hydrogel, synthesized via copolymerization of acrylamide and PR-PEGMA crosslinker with PEDOT:PSS as a conductive filler, exhibits tissue-matching elasticity (Young's modulus ≈118 kPa) and high conductivity (177 mS m−1). The AgInS2 semiconductor, in situ grown on the hydrogel surface, generates reactive oxygen species under visible light, triggering DA polymerization into polydopamine (PDA). This process establishes a self-enhancing feedback loop between AgInS2 and PDA, enabling selective DA detection with a linear range of 0.2–4 μM and limit of detection of 64 nM. Implanted into the mouse striatum, the sensor successfully tracked dynamic DA fluctuations induced by nomifensine maleate, demonstrating its capability for real-time in vivo neurochemical analysis. This work advances the development of minimally invasive, high-sensitivity tools for brain research.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 11","pages":" 2350-2355"},"PeriodicalIF":3.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectral imaging and a one-class classifier for detecting elastane in cotton fabrics† 用于检测棉织物中弹性纤维的光谱成像及一类分类器

IF 3.6 3区化学

Analyst Pub Date : 2025-04-22 DOI: 10.1039/D5AN00107B

Ella Mahlamäki, Inge Schlapp-Hackl, Tharindu Koralage, Michael Hummel and Mikko Mäkelä

{"title":"Spectral imaging and a one-class classifier for detecting elastane in cotton fabrics†","authors":"Ella Mahlamäki, Inge Schlapp-Hackl, Tharindu Koralage, Michael Hummel and Mikko Mäkelä","doi":"10.1039/D5AN00107B","DOIUrl":"10.1039/D5AN00107B","url":null,"abstract":"Elastane detection is important for textile recycling as elastane fibers can hamper mechanical and chemical fiber recycling. Here, we report the use of near-infrared imaging spectroscopy and class modelling to detect 2–6% elastane in consumer cotton fabrics to provide alternatives to current detection methods, which are invasive and time-consuming. Our method automatically identified outlier fabrics and measurements with class-specific clustering and showed higher classification accuracies by averaging across individual pixel spectra to reduce sampling uncertainty. The final classification results showed median test set true positive and true negative rates of 89–97% based on randomized resampling. Class modelling offers clear benefits compared to commonly used discriminant classifiers as it allows modelling new classes using only a set of target samples without requiring representative training objects from all the other classes. Overall, these results open the possibility for fast non-invasive detection of small amounts of elastane in cotton, taking us a step closer to a circular economy of textiles.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 11","pages":" 2295-2301"},"PeriodicalIF":3.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d5an00107b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal–organic framework decorated whispering-gallery-mode microsphere cavities for VOC sensing† 金属有机框架装饰的微球腔用于VOCs检测

IF 3.6 3区化学

Analyst Pub Date : 2025-04-22 DOI: 10.1039/D5AN00102A

Xianggang Chen, Xiaoyan Zhang, Xiaoyi Wu, Ling Bai and Yin Yin

引用次数: 0

Sarin simulants show limited representativeness† 沙林模拟物的代表性非常有限

IF 3.6 3区化学

Analyst Pub Date : 2025-04-22 DOI: 10.1039/D5AN00225G

Valentin A. Bureau, Sébastien Penlou, Sonia Sousa Nobre, Alexandre Carella and Jean-Pierre Simonato

引用次数: 0

A GQD/g-C3N4-modified nanochip for electrochemical detection of dengue serotype 3† GQD/g- c3n4修饰纳米芯片电化学检测3型登革热

IF 3.6 3区化学

Analyst Pub Date : 2025-04-22 DOI: 10.1039/D5AN00035A

Renu Poria, Ankur Kaushal, Shruti Verma, Selva Kumar Ramasamy, Gajanan Ghodake, Deepak Kumar and Shagun Gupta

{"title":"A GQD/g-C3N4-modified nanochip for electrochemical detection of dengue serotype 3†","authors":"Renu Poria, Ankur Kaushal, Shruti Verma, Selva Kumar Ramasamy, Gajanan Ghodake, Deepak Kumar and Shagun Gupta","doi":"10.1039/D5AN00035A","DOIUrl":"10.1039/D5AN00035A","url":null,"abstract":"A graphene quantum dot/2D graphitic carbon nitride (GQD)/g-C3N4-based ultrasensitive DNA biosensor was constructed using 5′ aminated DNA probes immobilized onto the surface of a screen-printed paper electrode (SPPE) for detecting DENV 3 (dengue serotype 3). FTIR, UV-Vis, TEM, and particle size measurements were used to characterize the synthesized nanocomposites. These results were then used to fabricate an affordable DNA sensor based on an SPPE that detects DENV 3. Initially, bioinformatics methods were used to develop DNA probes unique to DENV 3. The findings indicated that the most effective DNA probe for identifying DENV 3 was a 20-nucleotide sequence, which was immobilized onto a GQD/g-C3N4-modified SPPE. The electrochemical response was studied via differential pulse voltammetry (DPV) and cyclic voltammetry (CV). The electrode surface at each step of fabrication was characterized via FE-SEM, EIS and FTIR. The developed DNA sensor showed excellent analytical performance in terms of LOD and sensitivity. In CV, the LOD was 1 pg μl−1, with a sensitivity of 7993 ng μA−1 mm−2. However, in DPV, the LOD and sensitivity were 1 pg μl−1 and 11 116 ng μA−1 mm−2, respectively. This method should be preferable in the future for detecting DENV 3 in biological and clinical samples.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 11","pages":" 2430-2439"},"PeriodicalIF":3.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic platform for rapid multiplexed biological sample handling for molecular interrogation† 用于分子询问的快速多路生物样品处理的微流控平台

IF 3.6 3区化学

Analyst Pub Date : 2025-04-22 DOI: 10.1039/D4AN01415D

Seth R. T. Zima, Rithvik V. Turaga, Sofia C. Yeates-Delahoz and Jose M. Ayuso

{"title":"Microfluidic platform for rapid multiplexed biological sample handling for molecular interrogation†","authors":"Seth R. T. Zima, Rithvik V. Turaga, Sofia C. Yeates-Delahoz and Jose M. Ayuso","doi":"10.1039/D4AN01415D","DOIUrl":"10.1039/D4AN01415D","url":null,"abstract":"Cell manufacture and immune cell research often require screening large numbers of analytes like proteins, metabolites, and nucleic acids, which usually entail expensive equipment and labor-intensive assays such as flow cytometry. On the other hand, microfluidic devices can generate numerous readouts with reduced sample volume, however they often require specialized equipment and expertise to fabricate and use. To this end, we have designed a microfluidic device based on a well plate design for multiplexed analysis of biochemical analyte readouts. The device enables simultaneous liquid delivery to multiple individual wells, thus reducing the number of pipetting steps required to perform assays. The capillary valves used to confine liquid within the wells are capable of withstanding freeze-thawing, which enables pre-loading of plates with reagent for storage and later use. We performed a bioluminescence ATP assay to quantify T cell viability and found that the device was able to capture the luminescence signal with a high degree of consistency. Finally, we performed a bioluminescence immunoassay to compare secretion of IFN-γ between naïve and activated CD4+ T cells, thereby highlighting the potential for this technology to measure relevant immune markers.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 11","pages":" 2302-2311"},"PeriodicalIF":3.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly sensitive and reproducible SERS substrate based on ordered multi-tipped Au nanostar arrays for the detection of myocardial infarction biomarker cardiac troponin I† 基于有序多端金纳米星阵列的高灵敏度和可重复性SERS底物检测心肌梗死生物标志物心肌肌钙蛋白I

IF 3.6 3区化学

Analyst Pub Date : 2025-04-18 DOI: 10.1039/D5AN00171D

Qing Xiang, Hao Wang, Shengdong Liu, Yilong Zheng, Shipan Wang, Huanhuan Zhang, Yonggang Min and Yuguang Ma

{"title":"Highly sensitive and reproducible SERS substrate based on ordered multi-tipped Au nanostar arrays for the detection of myocardial infarction biomarker cardiac troponin I†","authors":"Qing Xiang, Hao Wang, Shengdong Liu, Yilong Zheng, Shipan Wang, Huanhuan Zhang, Yonggang Min and Yuguang Ma","doi":"10.1039/D5AN00171D","DOIUrl":"10.1039/D5AN00171D","url":null,"abstract":"Acute myocardial infarction (AMI) is a severe cardiovascular disease, for which early diagnosis is critical for reducing mortality and improving patient outcomes. Cardiac troponin I (cTnI) is widely recognized as the “gold standard” biomarker for AMI due to its high specificity and sensitivity. The concentration of cTnI correlates directly with different stages of AMI. Therefore, the accurate detection of cTnI concentration is of paramount importance. However, the low concentration of cTnI in biological fluids requires ultrasensitive detection methods. In this study, we developed a sandwiched surface enhanced Raman scattering (SERS)-based biosensor composed of SERS-immune substrate, target antigen, and SERS nanotags and realized sensitive and accurate detection of cTnI. The SERS-immune substrate features an ordered, multi-tipped monolayer of Au nanostars fabricated using a three-phase interfacial self-assembly method and 4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid (HEPES) buffer modification. Compared to Au nanosphere SERS substrates, the Au nanostar SERS substrates exhibited about a 3-fold increase in Raman enhancement and demonstrated good uniformity and batch stability. This novel SERS detection platform, leveraging dual plasmonic enhancement from both the SERS-immune substrate and SERS nanotags, achieves detection of cTnI with a limit of detection (LOD) as low as 9.09 pg mL−1 and a relative standard deviation (RSD) as low as 11.24%. Thus, the Au nanostar SERS substrates developed in this study demonstrate significant potential for rapid and accurate detection of cTnI.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 11","pages":" 2239-2250"},"PeriodicalIF":3.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methylene-blue-encapsulated liposome for immobilization-free electrochemical immunoassay of interleukin-6 from nervous headache 亚甲基蓝封装脂质体用于神经性头痛白细胞介素-6 的无固定电化学免疫测定

IF 3.6 3区化学

Analyst Pub Date : 2025-04-17 DOI: 10.1039/D5AN00346F

Nan Lin, Xiao Han, Shuo Tian, Dianping Tang and Danfeng Zhang

{"title":"Methylene-blue-encapsulated liposome for immobilization-free electrochemical immunoassay of interleukin-6 from nervous headache","authors":"Nan Lin, Xiao Han, Shuo Tian, Dianping Tang and Danfeng Zhang","doi":"10.1039/D5AN00346F","DOIUrl":"10.1039/D5AN00346F","url":null,"abstract":"Interleukin-6 (IL-6) protects neurons by inhibiting the expression of factors related to neuronal injury in nervous headache patients. The development of rapid and sensitive IL-6 detection methods will be very advantageous for easing the pain of such patients. In this work, an immobilization-free immunodetection method is explored for the voltammetric screening of IL-6 in serum samples from nervous headache patients. Initially, methylene-blue-encapsulated liposomes (MBLS) labeled with anti-IL-6 detection antibodies are confined in an anti-IL-6 capture antibody-coated microplate through a sandwich-type immunoreaction, and subsequently subjected to lysis treatment. After that, the lytic solution is transferred into a detection cell including a Nafion-modified working electrode. Methylene blue molecules with positive charge are captured on the negatively charged Nafion membrane, thus generating a voltammetric signal. The voltammetric peak currents are relative to the amount of IL-6 in the solution. Under optimized experimental situations, MBLS-based split-type electrochemical sensing protocols have acceptable voltammetric currents for IL-6 from 0.01 to 100 pg mL−1, and allow screening at a concentration of IL-6 as low as 9.1 fg mL−1. The batch-to-batch coefficients of variation were ≤11.95%. Good anti-interference capability was achieved against other biomolecules. Seven human serum specimens and two diluted serum samples including IL-6 obtained from nervous headache patients were determined by MBLS-based electrochemical immunoassay, and achieved well-matched results in comparison with those of the IL-6 ELISA protocol.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 11","pages":" 2343-2349"},"PeriodicalIF":3.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0