Analyst最新文献

{"title":"Shedding new light on the hidden complexity of seeds: chemically selective imaging of seed coats with stimulated Raman scattering microscopy","authors":"Chun-Chin Wang, Steven Penfield and Julian Moger","doi":"10.1039/D4AN01409J","DOIUrl":"10.1039/D4AN01409J","url":null,"abstract":"<p >The seed coat plays a pivotal role in seed development and germination, acting as a protective barrier and mediating interac-tions with the external environment. Traditional histochemical techniques and analytical methods have provided valuable insights into seed coat composition and function. However, these methods often suffer from limitations such as indirect chemical signatures and lack of spatial resolution. Here, we introduce stimulated Raman scattering (SRS) microscopy as a novel analytical tool for non-destructive, label-free, high-resolution mapping of biopolymers, water and applied active ingre-dients (AIs) in intact seed coats. We demonstrate the capability of SRS microscopy to perform depth-resolved, chemically selective imaging of major seed coat biopolymers (pectin, tannin, and suberin). By comparing wild type arabidopsis thali-ana seeds with genetically modified mutants deficient in suberin and tannin, we illustrate the potential for semi-quantitative analysis of biopolymer content. Furthermore, we show that SRS microscopy can track the permeability of seed coats to wa-ter using deuterated water (D<small>₂</small>O) uptake studies. Real-time imaging reveals differences in water permeation between wild type and suberin deficient seeds, highlighting the importance of seed coat composition in regulating water uptake during germina-tion. Additionally, we extend the application of SRS microscopy to large seeds, such as brassica oleracea, utilizing <em>epi</em>-detected imaging for surface studies. Finally, using a deuterated insecticide (clothianidin-d3), we demonstrate the capability of SRS microscopy to visualize the incorporation of AIs into seed coats. Our study presents SRS microscopy as a powerful tool for characterizing seed coat composition and understanding the diffusion of low molecular weight compounds into seeds. This technique offers new opportunities for designing seeds with tailored properties for improved germination and resilience to environmental stressors.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 3","pages":" 498-504"},"PeriodicalIF":3.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d4an01409j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Recent advances and applications to cultural heritage using ATR-FTIR spectroscopy and ATR-FTIR spectroscopic imaging","authors":"Guan-Lin Liu and Sergei G. Kazarian","doi":"10.1039/D4AN90095B","DOIUrl":"10.1039/D4AN90095B","url":null,"abstract":"<p >Correction for ‘Recent advances and applications to cultural heritage using ATR-FTIR spectroscopy and ATR-FTIR spectroscopic imaging’ by Guan-Lin Liu <em>et al.</em>, <em>Analyst</em>, 2022, <strong>147</strong>, 1777–1797, https://doi.org/10.1039/D2AN00005A.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 436-436"},"PeriodicalIF":3.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d4an90095b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive detection of nonfluorescent solutes in small amounts of dilute aqueous solutions through photothermally induced reflectivity modulation at glass/aqueous solution interfaces†","authors":"Shu-hei Urashima and Ryoji Kusaka","doi":"10.1039/D4AN01247J","DOIUrl":"10.1039/D4AN01247J","url":null,"abstract":"<p >In this study, we propose employing photothermal reflectance (PTR) spectroscopy, typically used to assess the thermal properties of solid materials, for detecting nonluminescent solutes in small amounts of dilute aqueous solutions. In this spectroscopy, a probe beam nonresonant and a pump beam resonant to the solution are focused on a transparent material/aqueous solution interface. As the temperature coefficients of refractive indexes depend on the media, the heat from the pump beam absorption changes the reflectivity at the material/solution interface. A limit of detection of 75 nM was achieved for an interface at a glass and an aqueous solution of a dye. Among various photothermal spectroscopic techniques, a notable advantage of reflectance spectroscopy is its straightforward optical configuration, where neither a precisely aligned beam expander nor a differential beam splitting is required, making the optical system inexpensive and reproducible. Since reflectance spectroscopy only requires a material/solution interface, it is applicable to solutions confined to micrometer-sized channels or pits, enabling the analysis of samples with trace amounts.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 5","pages":" 819-826"},"PeriodicalIF":3.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemistry and Electroanalytical Approaches","authors":"Damien W. M. Arrigan, Karolien De Wael, Jeffrey E. Dick, Thiago Paixão and Yi-Lun Ying","doi":"10.1039/D4AN90096K","DOIUrl":"10.1039/D4AN90096K","url":null,"abstract":"<p >A graphical abstract is available for this content</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 219-219"},"PeriodicalIF":3.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy†","authors":"R. González-Vegas, O. Seksek, A. Bertho, J. Bergs, R. Hirayama, T. Inaniwa, N. Matsufuji, T. Shimokawa, Y. Prezado, I. Yousef and I. Martínez-Rovira","doi":"10.1039/D4AN01038H","DOIUrl":"10.1039/D4AN01038H","url":null,"abstract":"<p >Radioresistant tumours remain complex to manage with current radiotherapy (RT) techniques. Heavy ion beams were proposed for their treatment given their advantageous radiobiological properties. However, previous studies with patients resulted in serious adverse effects in the surrounding healthy tissues. Heavy ion RT could therefore benefit from the tissue-sparing effects of minibeam radiation therapy (MBRT). To investigate the potential of this combination, here we assessed the biochemical response to neon MBRT (NeMBRT) through synchrotron-based Fourier transform infrared microspectroscopy (SR-FTIRM). Healthy (BJ) and tumour (B16-F10) cell lines were subjected to seamless (broad beam) neon RT (NeBB) and NeMBRT at HIMAC. SR-FTIRM measurements were conducted at the MIRAS beamline of ALBA Synchrotron. Principal component analysis (PCA) permitted to assess the biochemical effects after the irradiations and 24 hours post-irradiation for the different RT modalities and doses. For the healthy cells, NeMBRT resulted in the most dissimilar spectral signatures from non-irradiated cells early after irradiations, mainly due to protein conformational modifications. Nevertheless, most of the damage appeared to recover one day post-RT; conversely, protein- and nucleic acid-related IR bands were strongly affected by NeBB 24 hours after treatment, suggesting superior oxidative damage and nucleic acid degradation. Tumour cells appeared to be less sensitive to NeBB than to NeMBRT shortly after RT. Still, after one day, both NeBB and the high-dose NeMBRT regions yielded important spectral modifications, suggestive of cell death processes, protein oxidation or oxidative stress. Lipid-associated spectral changes, especially due to the NeBB and NeMBRT peak groups for the tumour cell line, were consistent with reactive oxygen species attacks.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 342-352"},"PeriodicalIF":3.6,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d4an01038h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproducible protein quantitation of 270 human proteins at increased depth using nanoparticle-based fractionation and multiple reaction monitoring mass spectrometry with stable isotope-labelled internal standards†","authors":"Claudia Gaither, Robert Popp, Aaron S. Gajadhar and Christoph H. Borchers","doi":"10.1039/D4AN00967C","DOIUrl":"10.1039/D4AN00967C","url":null,"abstract":"<p >Here we show that when using a mix of 274 light synthetic peptide standards (NAT) as surrogates for 270 human plasma proteins, as well as stable isotope-labelled standards (SIS) as normalizers (both from MRM Proteomics Inc.) for targeted quantitative analysis by liquid chromatography multiple reaction monitoring mass spectrometry (LC/MRM-MS), the Seer Proteograph™ platform allowed for the enrichment and absolute quantitation of up to an additional 62 targets (median) compared to two standard proteomic workflows without enrichment, representing an increase of 44%. The nanoparticle-based fractionation workflow resulted in improved reproducibility compared to a traditional proteomic workflow with no fractionation (median 8.3% <em>vs.</em> 13.1% CV). As expected, the protein concentrations in nanoparticle coronas were higher and had more compressed dynamic range in comparison to the concentrations determined either by a 3-hour Trypsin/LysC or overnight tryptic digestion methods. As the nanoparticle-based fractionation technology gains popularity, additional steps such as establishing technique-specific protein reference ranges across plasma samples and comparisons to well-established protein quantitation methods like enzyme-linked immunosorbent assay (ELISA) and LC/MRM-MS may be explored to enable absolute quantification of plasma proteins based on nanoparticle-based fractionation data.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 353-361"},"PeriodicalIF":3.6,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d4an00967c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An enzyme-free immunosorbent assay of staphylococcal enterotoxin B using a three-way DNA junction amplifier with an automatic reset function†","authors":"Wanhao Li, Jiali Xing, Jinzhong Xi, Shaofeng Yuan, Zhilong Yu, Hang Yu, Yuliang Cheng, Yahui Guo, Yunfei Xie and Weirong Yao","doi":"10.1039/D4AN01370K","DOIUrl":"10.1039/D4AN01370K","url":null,"abstract":"<p >A highly sensitive immunoadsorption assay without traditional horseradish peroxidase for signal amplification was developed, utilizing a three-way DNA junction amplifier instead. The formation of a three-way DNA junction and release of trigger DNA with recycling was accomplished by toehold-mediated strand displacement. The fluorescent dye <em>N</em>-methyl mesoporphyrin IX, with highly structure-specific binding affinity towards G-quadruplexes, was employed for label-free and simple signal output. High sensitivity was achieved for concentrations as low as 7.21 pg mL<small>⁻¹</small> by targeting Staphylococcal enterotoxin B for detection, and its applicability was confirmed by a recovery study of precooked dishes.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 4","pages":" 605-611"},"PeriodicalIF":3.6,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of essential oil diffusion matrices using non-ionic surfactants-supported NADES and hydrophobic NADES†","authors":"Samir Scandar, Natali Rianika Mustafa, Claudia Zadra, Maria Carla Marcotullio and Young Hae Choi","doi":"10.1039/D4AN01161A","DOIUrl":"10.1039/D4AN01161A","url":null,"abstract":"<p >Natural deep eutectic solvents (NADES) represent a significant advance in green chemistry, offering an eco-friendly alternative to conventional organic solvents for applications in extraction, reaction media, and formulations. This study explores the application of NADES in essential oil formulations, using lavender essential oil (LEO) to investigate the solubilization and release of volatile organic compounds (VOCs). Two distinct NADES formulations were evaluated: hydrophilic NADES combined with surfactants, and hydrophobic NADES. The results show that hydrophilic NADES, when combined with non-ionic surfactants, form stable emulsions that effectively solubilize and release LEO's VOCs, overcoming the high hydrophilicity limitation of NADES. Headspace analysis and gas chromatography-mass spectrometry (GC-MS) identified 25 VOCs, with differential components analyzed through orthogonal partial least-squares discriminant analysis (OPLS-DA). VOCs were well-clustered in OPLS-DA and principal component analysis (PCA), with five clearly differentiated groups (C, N1, N3, N5, and N9). However, challenges remain, particularly in the solubilization and gradual release of VOCs. In contrast, hydrophobic NADES demonstrated a controlled release mechanism, effectively encapsulating and steadily releasing VOCs, making them advantageous for sustained fragrance delivery. The study further emphasizes the importance of the Hydrophilic–Lipophilic Balance (HLB) in NADES formulations, showing that adjusting HLB through surfactant concentrations significantly influences VOC release dynamics. In conclusion, this research highlights the potential of NADES as efficient, eco-friendly solvent systems for essential oil applications. By providing insights into optimizing NADES compositions for specific solubility and release profiles, the study identifies both challenges and opportunities, paving the way for further advancements in green chemistry.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 425-434"},"PeriodicalIF":3.6,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low background electrochemical sensor based on HCR towards acute myocardial infarction-specific miRNA detection†","authors":"Yan Liu, Ziqi Liu, Tingxiu Yan, Luyao Feng, Na He, Lu Tao, Li-Ping Xu and Xueji Zhang","doi":"10.1039/D4AN01065E","DOIUrl":"10.1039/D4AN01065E","url":null,"abstract":"<p >Acute myocardial infarction (AMI) accounts for a significant proportion of global fatalities, and early detection is crucial for improving patient outcomes. However, current diagnostic methods often struggle to detect AMI in its early stages. Herein, we present an electrochemical sensor utilizing a fractal gold (FracAu) electrode and hybridization chain reaction (HCR) amplification technology to detect AMI-specific microRNAs (miRNAs). When the target sequence was added, the HCR was triggered, leading to the formation of a long-nicked DNA double helix that efficiently captured a larger quantity of positively charged RuHex molecules, resulting in significant electrochemical signal amplification. More importantly, to avoid false positive signals, exonuclease I (Exo I) was introduced to selectively cleave single-stranded DNA (ssDNA) probes. These ssDNA probes, underwent random hydrolysis from hpDNA probes, could hybridize with helper DNA1 in the absence of the target, initiating the HCR process and producing a false positive signal. The inclusion of Exo I effectively avoided false positive signals and reduced background noise. Under optimized conditions, the fabricated sensor exhibited significant sensitivity and selectivity, showing a broad linear detection range from 10 pM to 10 nM and a low limit of 0.9 fM. The fabricated electrochemical sensor also successfully detected AMI-specific miRNA in real serum samples, underscoring its diagnostic promise. By providing a reliable tool for early detection, the innovative sensor holds significant potential in combating global cardiovascular disease-related mortality rates.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 362-370"},"PeriodicalIF":3.6,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of a titanium-functionalized polymeric material rich in hydrophilic groups for phosphoproteome and glycoproteome analyses in serum†","authors":"Xiuqin Sheng, Jiakai Chen, Jiahui Shao, Xiaoya Zhang, Bing Wang, Chuan-Fan Ding and Yinghua Yan","doi":"10.1039/D4AN01195C","DOIUrl":"10.1039/D4AN01195C","url":null,"abstract":"<p >The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and <em>N</em>,<em>N</em>-methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic groups. The material's hydrophilicity was further enhanced by a ring-opening reaction with cysteine (the product was denoted as Cys-PVEM). Finally, titanium sulfate was combined with Cys-PVME to form titanium-rich polymers (<strong>Cys-PVME-Ti<small>⁴⁺</small></strong>) for the enrichment of phosphopeptides and glycopeptides. <strong>Cys-PVME-Ti<small>⁴⁺</small></strong> has a good sensitivity (0.02 fmol) and selectivity (1 : 1000) with a loading capacity of 62 mg g<small>⁻¹</small>, recyclability (9 cycles), and a good recovery rate (101.6 ± 0.60%) for phosphopeptides, and good sensitivity (0.01 fmol μL<small>⁻¹</small>), selectivity (1 : 2000), a loading capacity of 62.5 mg g<small>⁻¹</small>, recyclability (9 cycles), and a good recovery rate (98.7 ± 1.2%) for glycopeptides. In addition, after enrichment with this material, 27 phosphopeptides with 14 phosphoproteins and 223 glycopeptides associated with 88 glycoproteins were captured from the serum of colorectal cancer patients, while 27 phosphopeptides associated with 14 phosphoproteins and 210 glycopeptides associated with 111 glycoproteins were also captured from the serum of a normal control. Gene ontology (GO) analysis revealed that complement activation, extracellular region, extracellular space, blood coagulation, the IgG immunoglobulin complex, and heparin binding were different between normal control and colorectal cancer, implying that related pathways are likely involved in colorectal cancer pathogenesis.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 395-404"},"PeriodicalIF":3.6,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}