Journal of Proteome Research最新文献

Human-Derived Proteome Characterization of Tongue Coating in Colorectal Cancer. 结直肠癌患者舌苔的蛋白质组学特征。

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-05-16 DOI: 10.1021/acs.jproteome.5c00120

Qubo Chen, Wanhua Li, Xiangyu Gu, Fengye Lin, Ying Chen, Hairong Su, Tao Su, Ang Li, Chuyang Wang, Xuan Zeng, Weicheng Chen, Beiping Zhang, Haiyan Zhang, Meng Gong

{"title":"Human-Derived Proteome Characterization of Tongue Coating in Colorectal Cancer.","authors":"Qubo Chen, Wanhua Li, Xiangyu Gu, Fengye Lin, Ying Chen, Hairong Su, Tao Su, Ang Li, Chuyang Wang, Xuan Zeng, Weicheng Chen, Beiping Zhang, Haiyan Zhang, Meng Gong","doi":"10.1021/acs.jproteome.5c00120","DOIUrl":"https://doi.org/10.1021/acs.jproteome.5c00120","url":null,"abstract":"Most colorectal cancer (CRC) patients experience changes in tongue coating morphology, but the underlying mechanisms remain unclear. While the human-derived proteome of tongue coatings in gastric cancer and healthy individuals has been characterized, research on CRC patients remains lacking. The tongue coating collection process is painless and noninvasive, providing a more favorable examination experience. This study aims to preliminarily explore the composition and changes in the human-derived proteome of tongue coatings in CRC patients, providing insights into abnormal morphology and potential new CRC screening methods. Utilizing a \"bottom-up\" strategy and data-independent acquisition (DIA) mode, the human-derived proteome of tongue coating in healthy controls, colorectal hyperplastic polyps patients, and CRC patients was detected using the EASY-nLC 1200 chromatograph coupled with the Orbitrap Fusion Lumos Tribrid mass spectrometer. Differentially expressed proteins were validated by Western blot, and the diagnostic efficacy of tongue coating proteins compared with CRC serum markers was assessed. Our results indicate that the human tongue coating proteome undergoes significant changes in CRC, with upregulated proteins potentially involved in remodeling the tongue coating morphology. Hemopexin (HPX), fibrinogen β chain (FGB), and cystatin C (CST3) in the tongue coating are promising indicators for CRC screening.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Omics Reveals Unanticipated Metabolic Rearrangements in a High-Oil Mutant of Plastid Acetyl-CoA Carboxylase. 比较组学揭示了质体乙酰辅酶a羧化酶高油突变体中意想不到的代谢重排。

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-05-16 DOI: 10.1021/acs.jproteome.4c00947

Amr Kataya, Jose Roberto S Nascimento, Chunhui Xu, Matthew G Garneau, Somnath Koley, Athen Kimberlin, Thiya Mukherjee, Brian P Mooney, Dong Xu, Philip D Bates, Doug K Allen, Abraham J Koo, Jay J Thelen

{"title":"Comparative Omics Reveals Unanticipated Metabolic Rearrangements in a High-Oil Mutant of Plastid Acetyl-CoA Carboxylase.","authors":"Amr Kataya, Jose Roberto S Nascimento, Chunhui Xu, Matthew G Garneau, Somnath Koley, Athen Kimberlin, Thiya Mukherjee, Brian P Mooney, Dong Xu, Philip D Bates, Doug K Allen, Abraham J Koo, Jay J Thelen","doi":"10.1021/acs.jproteome.4c00947","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c00947","url":null,"abstract":"Heteromeric acetyl-CoA carboxylase (ACCase) catalyzes the ATP-dependent carboxylation of acetyl-CoA to produce malonyl-CoA, which is the committed step for de novo fatty acid synthesis. In plants, ACCase activity is controlled at multiple levels, including negative regulation by biotin attachment domain-containing (BADC) proteins, of which the badc1/3 double mutant leads to increased seed triacylglycerol accumulation. Unexpectedly, the Arabidopsis badc1/3 mutant also accumulated more protein. The metabolic consequences from both higher oil and protein were investigated in developing badc1/3 seed using global transcriptomics, translatomics, proteomics, metabolomics, and other biomass measurements. Changes included increased storage proteins and lipid droplet-packaging proteins, increased SDP1 lipase, altered organic acid metabolism, and reduced extracellular lipid synthesis perhaps offsetting the increase in TAG. We present a model of how Arabidopsis adapted to deregulated ACCase, resulting in more oil, and altered flux through pathways that partition carbon and propose targets for future bioengineering of seed storage reserves.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Proteomic Analysis Reveals Altered Ciliary Proteins in Sickle Cell Disease. 比较蛋白质组学分析揭示镰状细胞病纤毛蛋白改变

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-05-15 DOI: 10.1021/acs.jproteome.5c00168

Ashraf M Mohieldin, Madison Spencer, Carter Bernal, Wala B Fadol, Ankan Gupta, Karthikeyan Thirugnanam, Claire Delahunty, Francisco Nunez, Amy Y Pan, Amanda M Brandow, Sean P Palecek, Kevin R Rarick, Ramani Ramchandran, Rahima Zennadi, John Yates, Surya M Nauli

{"title":"Comparative Proteomic Analysis Reveals Altered Ciliary Proteins in Sickle Cell Disease.","authors":"Ashraf M Mohieldin, Madison Spencer, Carter Bernal, Wala B Fadol, Ankan Gupta, Karthikeyan Thirugnanam, Claire Delahunty, Francisco Nunez, Amy Y Pan, Amanda M Brandow, Sean P Palecek, Kevin R Rarick, Ramani Ramchandran, Rahima Zennadi, John Yates, Surya M Nauli","doi":"10.1021/acs.jproteome.5c00168","DOIUrl":"https://doi.org/10.1021/acs.jproteome.5c00168","url":null,"abstract":"Sickle cell disease (SCD) is an inherited hemoglobinopathy characterized by sickle-shaped red blood cells (RBCs). Primary cilia are mechanosensory organelles and are projected in the lumen of blood vessels to detect blood flow. We previously reported that interaction between microvasculature endothelial cells and sickled RBCs resulted in altered blood flow that can elevate reactive oxygen species, leading to increased deciliation in SCD patients. However, the impact of deciliation mediated by sickled RBCs in the context of the ciliary protein profiles remains unclear. Here, we investigated cell-cilia stability under different physiological shear-stress magnitudes and examined cilia protein profiles in SCD, utilizing mouse models and human participants. Our results demonstrate that subjecting endothelial cilia to sickled RBCs at 5.0 dyn/cm2 led to significant deciliation events. The proteomic and bioinformatic analyses showed different ciliary protein profiles, distinct signaling pathways, and unique post-translational modification processes in the SCD mouse model. Consistent with the SCD mouse model results, our translational studies validated the enrichment of specific proteins, including Transferrin Receptor-1 (TfR1), Glyceraldehyde-3-Phosphate-Dehydrogenase (GAPDH), and ADP Ribosylation Factor Like GTPase-13B (ARL13B) in SCD patients. These findings underscore the clinical relevance of cilia in SCD and suggest that ciliary proteins are potential biomarkers for assessing vascular damage.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative Proteome Analysis of Plasma Extracellular Vesicles Identifies Three Proteins with Potential Diagnostic Value for Mycobacterium bovis Infection in Cows. 血浆细胞外囊泡定量蛋白质组学分析鉴定出三种可能诊断牛分枝杆菌感染的蛋白质。

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-05-15 DOI: 10.1021/acs.jproteome.5c00143

Hangfan Zhou, Wenhui Wu, Qilong Zhang, Tao Zhang, Songhao Jiang, Hui Liu, Yuan Ma, Lei Chang, Yuping Xie, Jiaqiang Zhu, Degang Zhou, Yao Zhang, Ping Xu

{"title":"Quantitative Proteome Analysis of Plasma Extracellular Vesicles Identifies Three Proteins with Potential Diagnostic Value for Mycobacterium bovis Infection in Cows.","authors":"Hangfan Zhou, Wenhui Wu, Qilong Zhang, Tao Zhang, Songhao Jiang, Hui Liu, Yuan Ma, Lei Chang, Yuping Xie, Jiaqiang Zhu, Degang Zhou, Yao Zhang, Ping Xu","doi":"10.1021/acs.jproteome.5c00143","DOIUrl":"https://doi.org/10.1021/acs.jproteome.5c00143","url":null,"abstract":"Bovine tuberculosis (bTB) is a zoonotic disease that affects cattle and human health. Although the tuberculin skin test (TST) is the main detection method, there is a need for simpler on-farm tests using fluid samples. This study analyzed plasma extracellular vesicles (EVs) from two cow groups (Cohort A, 15 negative, 22 positive; Cohort B, 28 negative, 40 positive) to explore bTB indicators using proteome profiling. Among the 756 proteins, 217 (Cohort A) and 233 (Cohort B) showed differences between healthy and infected cows, with 47 consistently dysregulated in both groups. These proteins were related to tuberculosis, neutrophil extracellular trap formation, and antigen processing and presentation pathways. Notably, three proteins, HSPA8, B2M, and HRG, were confirmed as bTB indicators using multiple methods, including least absolute shrinkage and selection operator (Lasso) regression selection, western blot (WB), and enzyme-linked immunosorbent assay (ELISA) validation with an independent cohort (Cohort C). This study identifies plasma EV biomarkers for bTB infection, offering insights for bTB detection.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycoproteomics of Gastrointestinal Cancers and Its Use in Clinical Diagnostics. 胃肠道肿瘤的糖蛋白组学及其在临床诊断中的应用。

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-05-14 DOI: 10.1021/acs.jproteome.5c00095

Tomas Bertok, Andrea Pinkeova, Lenka Lorencova, Anna Datkova, Michal Hires, Eduard Jane, Jan Tkac

引用次数: 0

Technical Evaluation of Plasma Proteomics Technologies. 血浆蛋白质组学技术评价

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-05-14 DOI: 10.1021/acs.jproteome.5c00221

William F Beimers, Katherine A Overmyer, Pavel Sinitcyn, Noah M Lancaster, Scott T Quarmby, Joshua J Coon

{"title":"Technical Evaluation of Plasma Proteomics Technologies.","authors":"William F Beimers, Katherine A Overmyer, Pavel Sinitcyn, Noah M Lancaster, Scott T Quarmby, Joshua J Coon","doi":"10.1021/acs.jproteome.5c00221","DOIUrl":"https://doi.org/10.1021/acs.jproteome.5c00221","url":null,"abstract":"Plasma proteomics technologies are rapidly evolving and of critical importance to the field of biomedical research. Here, we report a technical evaluation of six notable plasma proteomics technologies─unenriched (Neat), acid depletion, PreOmics ENRICHplus, Mag-Net, Seer Proteograph XT, and Olink Explore HT. The methods were compared on proteomic depth, reproducibility, linearity, tolerance to lipid interference, and limit of detection/quantification. In total, we performed 618 LC-MS/MS experiments and 93 Olink Explore HT assays. The Seer method achieved the greatest proteomic depth (∼4500 proteins detected), while Olink detected ∼2600 proteins. Other MS-based methods ranged from ∼500-2200 proteins detected. In our analysis, Neat, Mag-Net, Seer, and Olink had good reproducibility, while PreOmics and Acid had higher variability (>20% median coefficient of variation). All MS methods showed good linearity with spiked-in C-reactive protein (CRP); CRP was surprisingly not in the Olink assay. None of the methods were affected by lipid interference. Seer produced the highest number of quantifiable proteins with a measurable LOD (4407) and LOQ (2696). Olink had the next highest number of quantifiable proteins, with 2002 having an LOD and 1883 having an LOQ. Finally, we tested the applicability of these methods for detecting differences between healthy and cancer groups in a nonsmall cell lung cancer (NSCLC) cohort. All six methods detected differentially abundant proteins between the cancer and healthy samples but disagreed on which proteins were significant, highlighting the contrast between each method.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic Changes in Lymphocyte Subsets and Inflammation-Immune-Related Proteins in Patients with Thyroid Papillary Carcinoma before and after Radioactive Iodine Therapy. 放射性碘治疗前后甲状腺乳头状癌患者淋巴细胞亚群和炎症免疫相关蛋白的动态变化

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-05-14 DOI: 10.1021/acs.jproteome.5c00128

Junyu Zhang, Lulu Shi, Zhilin Zhang, Wanshu Peng, Peifen Chen, Jiaxin Cao, Jing Liu, Jin Zhang, Keyi Lu, Zhifang Wu, Haiyan Liu, Sijin Li

{"title":"Dynamic Changes in Lymphocyte Subsets and Inflammation-Immune-Related Proteins in Patients with Thyroid Papillary Carcinoma before and after Radioactive Iodine Therapy.","authors":"Junyu Zhang, Lulu Shi, Zhilin Zhang, Wanshu Peng, Peifen Chen, Jiaxin Cao, Jing Liu, Jin Zhang, Keyi Lu, Zhifang Wu, Haiyan Liu, Sijin Li","doi":"10.1021/acs.jproteome.5c00128","DOIUrl":"https://doi.org/10.1021/acs.jproteome.5c00128","url":null,"abstract":"Radioactive iodine therapy (RAIT) is one of the main treatment methods for patients with papillary thyroid carcinoma (PTC). Peripheral blood samples were collected from 29 PTC patients who underwent total thyroidectomy 1-2 days before RAIT (RAIT-0), 30 days after (RAIT-30), and 90 days after (RAIT-90). Flow cytometry was used to detect the absolute counts of peripheral blood lymphocyte subpopulations, and the Proximity Extension Assay (PEA) was used for targeted proteome quantification. We found that after RAIT, peripheral blood lymphocyte subpopulations and the abundance of inflammation-related proteins in PTC patients changed and did not return to pretreatment levels for a long time. The immune pathway \"Cytokine-cytokine receptor interaction\" related to cytokine signaling may be activated. In addition, there were two types of protein trends that were similar to the lymphocyte count trends. The five proteins with the greatest degree of trend change were MCP-1, TRAIL, TGF-alpha, Flt3L, and IL15. In RAIT-30 vs RAIT-0, B cells were significantly negatively correlated with protein Flt3L, and Th17 cells were significantly positively correlated with protein CRTAM. MCP-1, TRAIL, IL15, Flt3L, TGF-alpha, and CRTAM may be potential marker proteins for immune recovery in PTC patients after RAIT.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upregulations of SNAT2 and GLS-1 Are Key Osmoregulatory Responses of Human Corneal Epithelial Cells to Hyperosmotic Stress. 人角膜上皮细胞对高渗应激的关键渗透调节反应是上调SNAT2和GLS-1。

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-05-13 DOI: 10.1021/acs.jproteome.4c01046

Kenrick Kai-Yuen Chan, Alan Chun-Kit Lee, Shing-Yan Roy Chung, Man-Sau Wong, Chi-Wai Do, Thomas Chuen Lam, Hang-Kin Kong

{"title":"Upregulations of SNAT2 and GLS-1 Are Key Osmoregulatory Responses of Human Corneal Epithelial Cells to Hyperosmotic Stress.","authors":"Kenrick Kai-Yuen Chan, Alan Chun-Kit Lee, Shing-Yan Roy Chung, Man-Sau Wong, Chi-Wai Do, Thomas Chuen Lam, Hang-Kin Kong","doi":"10.1021/acs.jproteome.4c01046","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c01046","url":null,"abstract":"Dry eye syndrome (DES) affects millions of people worldwide. However, as the cellular responses of the corneal epithelium under hyperosmotic stress remain unclear, this study investigated the proteomic changes between human corneal epithelial cells (HCECs) cultured with isosmotic and hyperosmotic media. Under hyperosmotic stress, HCECs increased expressions of sodium-coupled neutral amino acid transporter (SNAT2), glutaminase (GLS-1), and a few isoforms of heat shock protein and aldo-keto reductase family 1. The expressions of SNAT2 and GLS-1 were increased after 6 h of exposure to hyperosmotic stress but not by glutamine deprivation. The hyperosmotic stress increased intracellular levels of glutamine, mitochondrial superoxide, and mitochondrial membrane potential and induced mitochondrial fission in HCECs. Thus, the intracellular level of glutamine was elevated in the hyperosmotic stressed HCECs via the upregulation of SNAT2. Glutamine can act as an osmolyte to regulate the osmolarity of HCECs or be converted to glutamate by GLS-1 for the tricarboxylic acid cycle and oxidative phosphorylation to maintain ATP production under the hyperosmotic stress-induced mitochondrial fission. Thus, the increases in the expressions of SNAT2 and GLS-1 are key osmoregulations in HCECs upon the hyperosmotic stress and may act as corneal biomarkers for monitoring DES progression.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ProteoPlotter: An Executable Proteomics Visualization Tool Compatible with Perseus. ProteoPlotter：一个可执行的蛋白质组可视化工具与珀尔修斯兼容。

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-05-13 DOI: 10.1021/acs.jproteome.4c00963

Esther Olabisi-Adeniyi, Jason A McAlister, Daniela Ferretti, Juergen Cox, Jennifer Geddes-McAlister

{"title":"ProteoPlotter: An Executable Proteomics Visualization Tool Compatible with Perseus.","authors":"Esther Olabisi-Adeniyi, Jason A McAlister, Daniela Ferretti, Juergen Cox, Jennifer Geddes-McAlister","doi":"10.1021/acs.jproteome.4c00963","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c00963","url":null,"abstract":"Mass spectrometry-based proteomics experiments produce complex data sets requiring robust statistical testing and effective visualization tools to ensure meaningful conclusions are drawn. The publicly available proteomics data analysis platform, Perseus, is extensively used to perform such tasks, but opportunities to enhance visualization tools and promote accessibility of the data exist. In this study, we developed ProteoPlotter, a user-friendly, executable tool to complement Perseus for visualization of proteomics data sets. ProteoPlotter is built on the Shiny framework for R programming and enables illustration of multidimensional proteomics data. ProteoPlotter supports mapping of one-dimensional enrichment analyses, enhanced adaptability of volcano plots through incorporation of Gene Ontology terminology, visualization of 95% confidence intervals in principal component analysis plots using data ellipses, and customizable features. ProteoPlotter is designed for intuitive use by biological and computational researchers alike, providing descriptive instructions (i.e., Help Guide) for preparing and uploading Perseus output files. Herein, we demonstrate the application of ProteoPlotter toward microbial proteome remodeling under altered nutrient conditions and highlight the diversity of visualizations enabled with the platform for enhanced biological insights. Through its comprehensive data visualization capabilities, linked to the power of Perseus data handling and statistical analyses, ProteoPlotter facilitates enhanced visualization of proteomics data to drive new biological discoveries.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Comparison of Methods for Isolation of Extracellular Vesicles from Human Plasma. 人血浆细胞外囊泡分离方法的综合比较。

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-05-12 DOI: 10.1021/acs.jproteome.5c00149

Patil Shivprasad Suresh, Qibin Zhang

{"title":"Comprehensive Comparison of Methods for Isolation of Extracellular Vesicles from Human Plasma.","authors":"Patil Shivprasad Suresh, Qibin Zhang","doi":"10.1021/acs.jproteome.5c00149","DOIUrl":"https://doi.org/10.1021/acs.jproteome.5c00149","url":null,"abstract":"Extracellular vesicles (EVs) are a vital component in cell-cell communication and hold significant potential as biomarkers and therapeutic carriers. Having a reproducible and simple EV isolation method for small volumes of human plasma is essential for biomarker discovery. Although combining multiple methods has been a recent trend in its ability to minimize contamination, it is not ideal for clinical specimens due to the large sample number and small sample volume. This study compared EVs isolated from 100 μL of plasma by nine commonly used methods based on different principles, including centrifugation, polymer precipitation, size exclusion, electrostatic interaction, and affinity enrichment. The isolated EVs were characterized by particle size and number using nanoparticle tracking analysis, purity, and contaminants using Simple Western and overall proteomic profiles using bottom-up proteomics. Despite the same EV enrichment principle, individual methods isolated EVs exhibited distinct characteristics, likely due to variations in the physicochemical properties of materials used and specific protocols. Overall, all of the methods evaluated are reproducible. MagNet and MagCap methods result in purer EVs with the narrowest size distribution and the highest proteome coverage but modest yield. This is the first report on isolating EVs from 100 μL of plasma using nine different methods with detailed characterization.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0