Journal of Proteome Research最新文献

Unlocking the Potential of Single-Cell Omics

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-04-04 DOI: 10.1021/acs.jproteome.5c0019710.1021/acs.jproteome.5c00197

Nikolai Slavov*,

引用次数: 0

Lipidomics Atlas Tracks Alterations Associated with Deltamethrin-Induced Developmental Neurotoxicity in Embryonic Zebrafish.

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-04-03 DOI: 10.1021/acs.jproteome.4c00779

Longhua Gao, Jingwen Hao, Zhengyi Hua, Changchun Zeng, Jia Li, Jun Zeng

{"title":"Lipidomics Atlas Tracks Alterations Associated with Deltamethrin-Induced Developmental Neurotoxicity in Embryonic Zebrafish.","authors":"Longhua Gao, Jingwen Hao, Zhengyi Hua, Changchun Zeng, Jia Li, Jun Zeng","doi":"10.1021/acs.jproteome.4c00779","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c00779","url":null,"abstract":"Deltamethrin (DM) is a widely used pyrethroid pesticide associated with childhood neurodevelopmental disorders. However, the specific impact of DM exposure during distinct early life stages remains unclear. Here, zebrafish embryos were exposed to DM at different stages: before (10-16 hpf), at the onset of (16-24 hpf), at the peak of (24-36 hpf) hypothalamic neurogenesis, and continuously from 10 to 120 hpf (subchronic exposure), using different dosages (1, 100, and 250 nM). Exposure to middle/high-dose DM at 24-36 and 10-120 hpf significantly reduced zebrafish locomotor activities and increased apoptotic cells in the spinal cord. As a pivotal factor in central nervous system disorder progression, altered lipid metabolism was investigated using nontargeted lipidomic analysis. DM exposure at 10-16 and 24-36 hpf led to the most significant lipidome reprogramming. Despite exhibiting a dose-dependent trend, even low-dose DM changed the lipidome. Cer 40:2;2 and PG 44:12 showed potential in identifying DM exposure effects. Significant changes in sphingolipid, cardiolipin, phosphatidylglycerol, and glycerolipid pathways were linked to DM-induced developmental neurotoxicity, indicating impaired membrane function, mitochondrial damage, and disrupted energy metabolism. Our study sheds new light on assessing early neurodevelopmental disturbances and identifying intervention targets, emphasizing sensitivity to DM during the critical early phase of neurodevelopment.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycoproteomics Analysis of Triple Wild-Type Lung Adenocarcinoma Tissue Samples.

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-04-02 DOI: 10.1021/acs.jproteome.4c01063

Simon Nándor Sugár, Balázs András Molnár, Fanni Bugyi, Gábor Kecskeméti, Zoltán Szabó, Ibolya Laczó, Tünde Harkó, Judit Moldvay, Lilla Turiák

{"title":"Glycoproteomics Analysis of Triple Wild-Type Lung Adenocarcinoma Tissue Samples.","authors":"Simon Nándor Sugár, Balázs András Molnár, Fanni Bugyi, Gábor Kecskeméti, Zoltán Szabó, Ibolya Laczó, Tünde Harkó, Judit Moldvay, Lilla Turiák","doi":"10.1021/acs.jproteome.4c01063","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c01063","url":null,"abstract":"Lung cancer has both high incidence and mortality, making it the leading cause of cancer-related mortality worldwide. It is a highly heterogeneous disease, with several histological subtypes and genetic alterations that influence prognosis and available treatment options. Here, we focus on the triple wild-type (TWT) subtype of lung adenocarcinoma (LUAD) that lacks the three most common actionable genetic alterations, subsequently making targeted therapies inaccessible. In this study, our aim was the mass spectrometry-based proteomic and N-glycoproteomic characterization of tumor and adjacent normal lung tissue regions from individuals (n = 12) with TWT LUAD. We found several proteins previously identified as potential prognostic or diagnostic biomarkers in LUAD and described dysregulated biological processes, giving an overview of the general differences between healthy and tumor tissue. Also, we highlight specific signatures detected using N-glycoproteomics and discuss their potential and importance based on data from databases and literature. To the best of our knowledge, this is the first N-glycoproteomics-focused study on TWT LUAD, and it could provide a valuable resource for further studies into this less well characterized subtype of lung cancer. For instance, we report altered N-glycosylation for several glycoproteins implicated in LUAD and other cancers that could have functional importance connected to the disease.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alteration of Ubiquitination in the Brain of ENOPH1 Knockout Mice after Early Ischemic Stroke.

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-04-01 DOI: 10.1021/acs.jproteome.4c00913

Yike Wu, Ping Tang, Zhengzheng Huang, Dayong Gu, Dewen Yan, Li Su, Yuan Zhang

{"title":"Alteration of Ubiquitination in the Brain of ENOPH1 Knockout Mice after Early Ischemic Stroke.","authors":"Yike Wu, Ping Tang, Zhengzheng Huang, Dayong Gu, Dewen Yan, Li Su, Yuan Zhang","doi":"10.1021/acs.jproteome.4c00913","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c00913","url":null,"abstract":"Enolase-phosphatase 1 (ENOPH1) is a newly identified enzyme associated with stress responses and neurodevelopmental disorders. Our previous study found that ENOPH1 mediates cerebral cell apoptosis and blood-brain barrier (BBB) dysfunction during early cerebral ischemia. Ubiquitination has been identified in neuronal damage and the neuroinflammatory response in ischemic stroke. However, whether ENOPH1 regulates ischemia-induced protein ubiquitination alteration is yet unclear. Hence, the present study explored changes in the ubiquitinomic in early ischemic brain tissues between wildtype and ENOPH1 knockout mice using a comprehensive quantitative analysis. Our results showed that 4000 ubiquitination-modified sites in 1613 proteins were quantified, with 772 ubiquitinated sites in 464 proteins significantly decreasing or increasing after ENOPH1 knockout (fold change >1.5 or <1/1.5, p < 0.05). When compared to our previous parallel proteome profiles, common differential proteins FKBP5 and Claudin-11 were observed and further validated. ENOPH1 regulates the degradation of FKBP5 and the promotion of Claudin-11 by ubiquitination mediation, leading to the activation or inhibition of nuclear-initiated steroid signaling and transendothelial migration pathways. These findings, for the first time, identified ubiquitinomic features of early ischemic brain tissues after ENOPH1 knockout, suggesting that ENOPH1 may regulate neuroinflammatory stress and barrier function by modifying FKBP5 and Claudin-11 protein ubiquitination.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How to Train a Postprocessor for Tandem Mass Spectrometry Proteomics Database Search While Maintaining Control of the False Discovery Rate.

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-03-31 DOI: 10.1021/acs.jproteome.4c00742

Jack Freestone, Lukas Käll, William Stafford Noble, Uri Keich

{"title":"How to Train a Postprocessor for Tandem Mass Spectrometry Proteomics Database Search While Maintaining Control of the False Discovery Rate.","authors":"Jack Freestone, Lukas Käll, William Stafford Noble, Uri Keich","doi":"10.1021/acs.jproteome.4c00742","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c00742","url":null,"abstract":"Decoy-based methods are a popular choice for the statistical validation of peptide detection in tandem mass spectrometry and proteomics data. Such methods can achieve a substantial boost in statistical power when coupled with postprocessors such as Percolator that use auxiliary features to learn a better-discriminating scoring function. However, we recently showed that Percolator can struggle to control the false discovery rate (FDR) when reporting the list of discovered peptides. To address this problem, we introduce Percolator-RESET, which is an adaptation of our recently developed RESET meta-procedure to the peptide detection problem. Specifically, Percolator-RESET fuses Percolator's iterative SVM training procedure with RESET's general framework to provide valid false discovery rate control. Percolator-RESET operates in both a standard single-decoy mode and a two-decoy mode, with the latter requiring the generation of two decoys per target. We demonstrate that Percolator-RESET controls the FDR in both modes, both theoretically and empirically, while typically reporting only a marginally smaller number of discoveries than Percolator in the single-decoy mode. The two-decoy mode is marginally more powerful than both Percolator and the single-decoy mode and exhibits less variability than the latter.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mind Your Spectra: Points to be Aware of When Validating the Identification of Isobaric Histone Peptidoforms.

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-03-31 DOI: 10.1021/acs.jproteome.4c01056

Hassan Hijazi, Julie Manessier, Sabine Brugiere, Tina Ravnsborg, Marie Courçon, Baptiste Brule, Karine Merienne, Ole N Jensen, Anne-Marie Hesse, Christophe Bruley, Delphine Pflieger

{"title":"Mind Your Spectra: Points to be Aware of When Validating the Identification of Isobaric Histone Peptidoforms.","authors":"Hassan Hijazi, Julie Manessier, Sabine Brugiere, Tina Ravnsborg, Marie Courçon, Baptiste Brule, Karine Merienne, Ole N Jensen, Anne-Marie Hesse, Christophe Bruley, Delphine Pflieger","doi":"10.1021/acs.jproteome.4c01056","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c01056","url":null,"abstract":"Mass spectrometry has become central to identifying and quantifying histone post-translational modifications (PTMs), surpassing limitations of antibody-based methods. Histones are dynamically modified by multiple structures, especially at lysine residues on their N-terminal tails, to regulate DNA-templated processes. Reliable identification of histone PTMs remains challenging and still requires manual curation. This study focused on the Lys27-Arg40 stretch of histone H3, considered four sequence variants, an increasing number of lysine PTMs and artifacts coming from histone sample processing, which resulted in many isobaric peptides. Our analysis revealed the value of low-mass b1 and cyclic immonium fragment ions to validate identification of the distinct peptidoforms. We examined how MS/MS spectra are transformed by common identification software during the conversion of raw files into peak lists, and highlighted how some parameters may erase the informative low-mass fragments. We targeted the detection of 40 H3 K27-R40 variant × PTM combinations, including the mouse-specific variants H3mm7 and H3mm13, in histone samples extracted from mouse testis and brain via a parallel reaction monitoring analysis. We only detected very low levels of unmodified H3mm7. Our work contributes to reliably deciphering the histone code shaped by distinct sequence variants and numerous combinations of PTMs.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microvesicles Derived from Human Bronchial Epithelial Cells Regulate Macrophage Activation During Mycobacterium abscessus Infection. 人支气管上皮细胞产生的微囊泡可在脓肿分枝杆菌感染过程中调节巨噬细胞的活化。

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-03-28 DOI: 10.1021/acs.jproteome.4c00827

Carlyn M Guthrie, Amber C Meeker, Ashton E Self, Aidaly Ramos-Leyva, Olivia L Clark, Stephen K Kotey, Steven D Hartson, Yurong Liang, Lin Liu, Xuejuan Tan, Yong Cheng

{"title":"Microvesicles Derived from Human Bronchial Epithelial Cells Regulate Macrophage Activation During Mycobacterium abscessus Infection.","authors":"Carlyn M Guthrie, Amber C Meeker, Ashton E Self, Aidaly Ramos-Leyva, Olivia L Clark, Stephen K Kotey, Steven D Hartson, Yurong Liang, Lin Liu, Xuejuan Tan, Yong Cheng","doi":"10.1021/acs.jproteome.4c00827","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c00827","url":null,"abstract":"Intercellular communication is important for host immunity in response to bacterial infections. Nontuberculous mycobacterium (NTM), such as Mycobacterium abscessus (M. ab), is a group of environmental bacteria that can cause severe lung infections in individuals with pre-existing lung conditions, including cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). There is limited knowledge understanding the interaction between airway epithelial cells and immune cells during NTM infections. In this study, we characterized microvesicles (MVs) released from uninfected and M. ab-infected human bronchial epithelial cells and investigated the effect of these MVs on the activation and polarization of THP-1-derived macrophages in cell culture. Our results indicate that MVs released by M. ab-infected human bronchial epithelial cells stimulated the activation of M2-polarized macrophages in cell culture when compared to MVs released by uninfected cells. Additionally, the proteomic analysis for isolated MVs showed that the proteins involved in the cell adhesion pathway were enriched in MVs from M. ab-infected human bronchial epithelial cells compared to MVs from uninfected cells. Among those, the cell surface protein, intercellular adhesion molecule 1 (ICAM-1), regulated the uptake of MVs released by M. ab-infected human bronchial epithelial cells by recipient macrophages in cell culture. In conclusion, our data suggest that in response to M. ab infection, human airway epithelial cells release MVs to modulate the activation of macrophages, which are key cells for mycobacterial intracellular survival in the host.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SpecPeptidOMS Directly and Rapidly Aligns Mass Spectra on Whole Proteomes and Identifies Peptides That Are Not Necessarily Tryptic: Implications for Peptidomics

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-03-27 DOI: 10.1021/acs.jproteome.4c0087010.1021/acs.jproteome.4c00870

Émile Benoist, Géraldine Jean*, Hélène Rogniaux, Guillaume Fertin and Dominique Tessier,

{"title":"SpecPeptidOMS Directly and Rapidly Aligns Mass Spectra on Whole Proteomes and Identifies Peptides That Are Not Necessarily Tryptic: Implications for Peptidomics","authors":"Émile Benoist, Géraldine Jean*, Hélène Rogniaux, Guillaume Fertin and Dominique Tessier, ","doi":"10.1021/acs.jproteome.4c0087010.1021/acs.jproteome.4c00870","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c00870https://doi.org/10.1021/acs.jproteome.4c00870","url":null,"abstract":"SpecPeptidOMS directly aligns peptide fragmentation spectra to whole and undigested protein sequences. The algorithm was specifically and initially designed for peptidomics, where the aim is to identify peptides that do not result from the hydrolysis of a known protein and therefore, whose termini cannot be predicted. Thus, SpecPeptidOMS can perform alignments starting and ending anywhere in the protein sequence. The underlying computational method of SpecPeptidOMS, which is based on a dynamic programming approach, was drastically optimized. As a result, SpecPeptidOMS can process around 12,000 spectra per hour on an ordinary laptop, with alignment performed against the entire human proteome. The performance of SpecPeptidOMS was first evaluated on a publicly available data set of (nontryptic) synthetic mass spectra. Accuracy was estimated by considering the results obtained by MaxQuant on the same data set as the “ground truth”. A second series of tests on a larger, well-known proteomics data set (HEK293) highlighted SpecPeptidOMS’ additional ability to search for open modifications, a feature of interest in peptidomics but also more broadly in conventional proteomics. SpecPeptidOMS is open-source, cross-platform (written in Java), and freely available.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"24 4","pages":"2159–2172 2159–2172"},"PeriodicalIF":3.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SpecPeptidOMS Directly and Rapidly Aligns Mass Spectra on Whole Proteomes and Identifies Peptides That Are Not Necessarily Tryptic: Implications for Peptidomics.

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-03-27 DOI: 10.1021/acs.jproteome.4c00870

Émile Benoist, Géraldine Jean, Hélène Rogniaux, Guillaume Fertin, Dominique Tessier

{"title":"SpecPeptidOMS Directly and Rapidly Aligns Mass Spectra on Whole Proteomes and Identifies Peptides That Are Not Necessarily Tryptic: Implications for Peptidomics.","authors":"Émile Benoist, Géraldine Jean, Hélène Rogniaux, Guillaume Fertin, Dominique Tessier","doi":"10.1021/acs.jproteome.4c00870","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c00870","url":null,"abstract":"SpecPeptidOMS directly aligns peptide fragmentation spectra to whole and undigested protein sequences. The algorithm was specifically and initially designed for peptidomics, where the aim is to identify peptides that do not result from the hydrolysis of a known protein and therefore, whose termini cannot be predicted. Thus, SpecPeptidOMS can perform alignments starting and ending anywhere in the protein sequence. The underlying computational method of SpecPeptidOMS, which is based on a dynamic programming approach, was drastically optimized. As a result, SpecPeptidOMS can process around 12,000 spectra per hour on an ordinary laptop, with alignment performed against the entire human proteome. The performance of SpecPeptidOMS was first evaluated on a publicly available data set of (nontryptic) synthetic mass spectra. Accuracy was estimated by considering the results obtained by MaxQuant on the same data set as the \"ground truth\". A second series of tests on a larger, well-known proteomics data set (HEK293) highlighted SpecPeptidOMS' additional ability to search for open modifications, a feature of interest in peptidomics but also more broadly in conventional proteomics. SpecPeptidOMS is open-source, cross-platform (written in Java), and freely available.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant Impact of Soft Knotwood Extracts on Human Keratinocytes Shown by NMR Metabolomic Analysis

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2025-03-26 DOI: 10.1021/acs.jproteome.4c0083610.1021/acs.jproteome.4c00836

Océane Quin, Marylène Bertrand*, Pauline Gerardin, Philippe Gerardin, Christine Gerardin-Charbonnier, Céline Landon and Chantal Pichon,

{"title":"Antioxidant Impact of Soft Knotwood Extracts on Human Keratinocytes Shown by NMR Metabolomic Analysis","authors":"Océane Quin, Marylène Bertrand*, Pauline Gerardin, Philippe Gerardin, Christine Gerardin-Charbonnier, Céline Landon and Chantal Pichon, ","doi":"10.1021/acs.jproteome.4c0083610.1021/acs.jproteome.4c00836","DOIUrl":"https://doi.org/10.1021/acs.jproteome.4c00836https://doi.org/10.1021/acs.jproteome.4c00836","url":null,"abstract":"The Pinophyta family has long been used to protect the skin from oxidation, thanks to the action of molecules such as stilbenes, flavonoids, and lignans, which are particularly concentrated in knotwood. These molecules are of interest from a cosmetic perspective. The present study focuses on four species from larch (Larix decidua Mill.), silver fir (Abies alba Mill.), Norway spruce (Picea abies (L.) H.Karst), and Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) knotwood, recovered from byproducts of the wood industry. The molecules are extracted from knotwood and used in vitro on human keratinocytes (HaCaT). Studies quantifying reactive oxygen species (ROS) have demonstrated its ability to eliminate hydroxyl radicals and superoxides. Metabolomic analyses using proton nuclear magnetic resonance (1H NMR) and multivariate statistics (PLS-DA) demonstrated that keratinocytes modulate metabolite expression after treatment with knot extracts. Indeed, our findings indicate an increase in metabolites such as glutathione, glycine, glutamate, sarcosine, taurine, and proline, which are known to reduce intracellular oxidative stress and validate the effect on ROS levels. They also indicate that knotwood extracts may affect membrane balance, collagen formation, and oxidative stress levels. This study highlights the value of metabolomic analysis in the cosmetic industry for a detailed understanding of the mechanisms implemented in a whole cell.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"24 4","pages":"1745–1756 1745–1756"},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0