ACS Nano最新文献

{"title":"Framework Nucleic Acid-Nanobody Fusion Probe-Based Pharmacokinetics Modulation and Analysis for Efficient Positron Emission Tomography Imaging","authors":"Yani Yang, Zhiqiang Ren, Dan Wang, Decui Tang, Weijun Wei, Shaoli Song, Yifan Lyu, Ding Ding, Weihong Tan","doi":"10.1021/acsnano.4c09127","DOIUrl":"https://doi.org/10.1021/acsnano.4c09127","url":null,"abstract":"Nanobodies are promising for immunoPET imaging due to their excellent antigen recognition and tumor targeting, yet rapid clearance limits their tumor accumulation. Although multimerization and albumin binding can extend their circulation time and improve tumor targeting, a simple and universal method for creating protein multimers is still needed. Here, we leveraged the facile synthesis, controllable size, and precise assembly of DNA nanotechnology to construct CD47-targeted framework nucleic acid-nanobody fusion probes with multiple valences and sizes. Following comprehensive structural characterization, in vitro specificity assessment and <i>in vivo</i> PET/CT imaging analysis were conducted on a colorectal cancer LS174T mouse model. Furthermore, a pharmacokinetic model was developed and fitted with considerable <i>in vivo</i> data to prove its rationality, followed by testing the effects on tumor uptake prediction by changing different pharmacokinetic parameters. Indeed, by manipulating the size of the nucleic acid scaffolding and the number of attached nanobodies, we could precisely modulate the accumulation of probes at the tumor site. Overall, this study not only developed an efficient strategy for constructing nanobody multimers but also provided a pharmacokinetic model, allowing profound insight into the multidimensional data obtained experimentally and informing the design of future imaging probes with predictable delivery efficacies.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"20 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinically-Driven Rapidly Developed Nanoparticle Corona for Label-Free Cerebrospinal Fluid Leakage Detection","authors":"Seungju Lee, Gwanghui Ryu, Seyoung Shin, Woojin Kim, Minyeong Yoon, Yeji Kim, Seongjun Park, YongJoo Kim, Soo-Yeon Cho","doi":"10.1021/acsnano.4c12364","DOIUrl":"https://doi.org/10.1021/acsnano.4c12364","url":null,"abstract":"Rapid diagnosis of cerebrospinal fluid (CSF) leaks is critical as endoscopic endonasal skull base surgery gains global prominence. Current clinical methods such as endoscopic examination with and without intrathecal injection of fluorescent dye are invasive and rely on subjective judgment by physicians, highlighting the clinical need for label-free point-of-care (POC). However, a viable solution remains undeveloped due to the molecular complexity of CSF rhinorrhea mixed with nasal discharge and the scarcity of specific biomarkers, delaying sensor development. In this study, we accelerated the development of a label-free CSF detection method for clinical use using a nanoparticle corona. We engineered corona nanointerfaces on near-infrared (nIR) fluorescent single-walled carbon nanotubes (SWCNTs) through noncovalent functionalization with 12 custom-designed poly(ethylene glycol) (PEG) lipids. By high-throughput screening of the corona library for the CSF biomarker β-trace protein (βTP), we selected the optimal corona, achieving a limit of detection (LOD) down to 1.46 mg/L, maintaining its selectivity even in human nasal discharge. Using molecular dynamics and docking simulations, we characterized the 3D morphology and βTP binding energy of the optimal corona in a quantified way. The corona nanosensor accurately diagnosed CSF leakages from eight patients having lumbar drainage and one patient with CSF leakage due to diverse diseases without any sample preparations. By integrating the nanosensor with custom-designed in vivo and in vitro form factors such as a camera and endoscope, we showed its potential for versatile and practical use in clinical settings. This accelerated sensor development platform can meet future urgent clinical demands for various diseases and conditions.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"28 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metasurface-Based Phosphor-Converted Micro-LED Architecture for Displays─Creating Guided Modes for Enhanced Directionality","authors":"Debapriya Pal, Toni López, A. Femius Koenderink","doi":"10.1021/acsnano.4c13472","DOIUrl":"https://doi.org/10.1021/acsnano.4c13472","url":null,"abstract":"Phosphor-converted micro-light emitting diodes (micro-LEDs) are a crucial technology for display applications but face significant challenges in light extraction because of the high refractive index of the blue pump die chip. In this study, we design and experimentally demonstrate a nanophotonic approach that overcomes this issue, achieving up to a 3-fold increase in light extraction efficiency. Our approach involves engineering the local density of optical states (LDOS) to generate quasi-guided modes within the phosphor layer by strategically inserting a thin low-index spacer in combination with a metasurface for mode extraction. We demonstrate the trade-offs between blue light pumping, LDOS enhancement at the converted emission wavelength, and radiation pattern control using a stratified system solver for dipole emission. Experimentally, the integration of plasmonic antennas and a silica spacer resulted in a 3-fold overall brightness enhancement, with nearly a 4-fold increase in forward emission. This nanophotonic metasurface waveguide design is a critical advancement for producing bright, directional micro-LEDs, particularly in augmented/virtual reality (AR/VR) devices and smartwatch displays, without the need for bulky secondary optics or reflectors.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"82 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cationic Magnetic Nanoparticles Activate Natural Killer Cells for the Treatment of Glioblastoma","authors":"Zhi-Yong Rao, Jing Kuang, Ting Pan, You-Teng Qin, Qian-Xiao Huang, Yu-Liang Sun, Kai Zhao, Xiao-Kang Jin, Chi-Hui Yang, Shi-Man Zhang, Yu Yan, Xian-Zheng Zhang","doi":"10.1021/acsnano.4c11250","DOIUrl":"https://doi.org/10.1021/acsnano.4c11250","url":null,"abstract":"The blood–brain barrier (BBB) and the immunosuppressive microenvironment of glioblastoma (GBM) severely hinder the infiltration and activity of natural killer (NK) cells, thereby reducing their clinical efficacy in GBM treatment. To address this challenge, we introduced an engineered living material, HEFDS-NK cells, designed to enhance the penetration of NK cells across the BBB and improve their cytotoxicity against GBM. HEFDS comprises magnetic nanoparticles modified using cationic polyethylenimine (PEI), selenocysteine (Sec), and sodium hyaluronate (HA) and cocultured with NK cells to form HEFDS-NK cells. With the assistance of HA and magnet targeting, HEFDS-NK cells can effectively cross the BBB and localize at the GBM site. Moreover, PEI enhances the expression of C–X–C chemokine receptor type 4 (CXCR4) and C–C chemokine receptor type 4 (CCR4) on NK cells, thereby improving their recognition and cytotoxicity against GBM. Additionally, Sec boosts the immune activity of NK cells against GBM. Upon recognizing GBM, the activated HEFDS-NK cells produce Granzyme B, Perforin, and IFN-γ, ultimately achieving effective therapy for GBM. This study demonstrates an effective treatment of GBM while enhancing NK cell activity and their ability to penetrate the BBB, providing an innovative and high-precision therapeutic approach for GBM.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"33 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Performance and Stable Perovskite/Organic Tandem Solar Cells Enabled by Interconnecting Layer Engineering","authors":"Songtao Liu, Lu Hao, Jiangkai Yu, Yao Xu, Yuejia Dou, Juxuan Xie, Yazhong Wang, Kai Zhang, Fei Huang, Yong Cao","doi":"10.1021/acsnano.4c11888","DOIUrl":"https://doi.org/10.1021/acsnano.4c11888","url":null,"abstract":"Perovskite/organic tandem solar cells (PO-TSCs) have recently attracted increasing attention due to their high efficiency and excellent stability. The interconnecting layer (ICL) is of great importance for the performance of PO-TSCs. The charge transport layer (CTL) and the charge recombination layer (CRL) that form the ICL should be carefully designed to enhance charge carrier extraction and promote charge carrier recombination balance from the two subcells. Here, we propose an effective strategy to optimize the ICL by using [2-(9<i>H</i>-carbazol-9-yl)ethyl]phosphonic acid (2PACz) to modify the poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS) as the hole transport layer (HTL) in the ICL. It is found that the coverage state of 2PACz on the PEDOT:PSS significantly affects the performance of PO-TSCs and can be regulated by adjusting the concentration of the 2PACz solution. The PEDOT:PSS/2PACz structure facilitates effective charge carrier extraction from the organic solar cells to the CRL. Herein, for the PO-TSCs, this strategy results in an efficient and balanced charge carrier recombination in the ICL and also allows a thinner PEDOT:PSS with reduced parasitic absorption. As a result, the PO-TSC achieves a power conversion efficiency (PCE) of 25.26%, much higher than the control device (PCE of 23.57%), and better stability. This work demonstrates an effective approach to achieving high-performance PO-TSCs through ICL engineering.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"112 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bicontinuous Block Copolymer Microparticles through Hydrogen-Bonding-Mediated Dual Phase Separation between Polymer Segments and Fluorinated Additives","authors":"Min Ren, Mengmeng Zhang, Zaiyan Hou, Xinghao Yan, Lianbin Zhang, Jiangping Xu, Jintao Zhu","doi":"10.1021/acsnano.4c13152","DOIUrl":"https://doi.org/10.1021/acsnano.4c13152","url":null,"abstract":"Bicontinuous microparticles have advanced transport, mechanical, and electrochemical properties and show promising applications in energy storage, catalysis, and other fields. However, it remains a great challenge to fabricate bicontinuous microparticles of block copolymers (BCPs) by controlling the microphase separation due to the extremely narrow region of a bicontinuous structure in the phase diagram. Here, we demonstrate a strategy to balance the phase separation of BCPs and fluorinated additives at different length scales in emulsion droplets, providing a large window to access bicontinuous microparticles. The key point is to simultaneously introduce contradictory attractive–repulsive interactions between poly(4-vinylpyridine)-containing BCPs and carboxylated perfluorinated additives. Hydrogen bonding between poly(4-vinylpyridine) and carboxyl groups, as an attractive interaction, directs the microphase separation between BCPs and additives. Meanwhile, the repulsive interaction due to the high immiscibility between perfluoroalkyl residues and BCPs induces macrophase separation. The compromise of attractive–repulsive interactions triggers the formation of bicontinuous microparticles in a large phase space. In addition, the vulnerable nature of hydrogen bonding provides a flexible route for reversibly shaping BCP assemblies. This work establishes a platform for fabricating structured BCP microparticles of which the structures are hardly accessible through traditional solution self-assembly.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"60 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechano-Graded Contact-Electrification Interfaces Based Artificial Mechanoreceptors for Robotic Adaptive Reception","authors":"Hao Lei, Yixin Cao, Guoxuan Sun, Peihao Huang, Xiyin Xue, Bohan Lu, Jiawei Yan, Yuxi Wang, Eng Gee Lim, Xin Tu, Yina Liu, Xuhui Sun, Chun Zhao, Zhen Wen","doi":"10.1021/acsnano.4c14285","DOIUrl":"https://doi.org/10.1021/acsnano.4c14285","url":null,"abstract":"Triboelectrification-based artificial mechanoreceptors (TBAMs) is able to convert mechanical stimuli directly into electrical signals, realizing self-adaptive protection and human–machine interactions of robots. However, traditional contact–electrification interfaces are prone to reaching their deformation limits under large pressures, resulting in a relatively narrow linear range. In this work, we fabricated mechano-graded microstructures to modulate the strain behavior of contact–electrification interfaces, simultaneously endowing the TBAMs with a high sensitivity and a wide linear detection range. The presence of step regions within the mechanically graded microstructures helps contact–electrification interfaces resist fast compressive deformation and provides a large effective area. The highly sensitive linear region of TBAM with 1.18 V/kPa can be effectively extended to four times of that for the devices with traditional interfaces. In addition, the device is able to maintain a high sensitivity of 0.44 V/kPa even under a large pressure from 40 to 600 kPa. TBAM has been successfully used as an electronic skin to realize self-adaptive protection and grip strength perception for a commercial robot arm. Finally, a high angle resolution of 2° and an excellent linearity of 99.78% for joint bending detection were also achieved. With the aid of a convolutional neural network algorithm, a data glove based on TBAMs realizes a high accuracy rate of 95.5% for gesture recognition in a dark environment.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"148 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Direct Excitation Transfer in Plasmonic Metal-Chalcopyrite Hybrids: Insights from Single Particle Line Shape Analysis”","authors":"Tianhong Ouyang, Yi-Chen Chen, Koustav Kundu, Xingjian Zhong, Yixin Mei, Akilesh Nalluri, Allison M. Dennis, Björn M. Reinhard","doi":"10.1021/acsnano.4c16477","DOIUrl":"https://doi.org/10.1021/acsnano.4c16477","url":null,"abstract":"The caption of Figure 4 in the text of our publication contains an error. Figure 4 does not contain a plot of all Γ_ET(ω) data as stated in the caption but of Γ_ET(ω) data for all AgNP@CuFeS₂ preparations with an average Γ_ET &gt; 0.3 eV. For completeness, we include below the Γ_ET(ω) plot for all AgNP@CuFeS₂ preparations. The interpolation (black line) contains a maximum at ω = 2.469 eV, which is nearly identical to the value of 2.489 eV that was determined from the original figure with the narrower Γ_ET distribution and does not change the interpretation of the data. We apologize for any confusion this error may have created. All authors have approved this correction. Figure 4. Γ_ET (blue points) for all AgNP@CuFeS₂ conditions plotted as a function of the resonance frequency ω_res. A polynomial interpolation is included as a black line. The red line shows the simulated scattering spectrum of an individual CuFeS₂ NC (<i>n</i>_r = 1.5000). This article has not yet been cited by other publications.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"31 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Carbon Dots/CaCO3/Abamectin Colloidal Pesticide Formulation for Safer and More Effective Pest Management","authors":"Chuang Chen, Guopeng Teng, Weicheng Shen, Yijun Lu, Yuwei Jin, Xue Yuan, Kang Chen, Yue Yuan, Zhengyan Wu, Jia Zhang","doi":"10.1021/acsnano.4c12707","DOIUrl":"https://doi.org/10.1021/acsnano.4c12707","url":null,"abstract":"An ideal green leaf-deposited pesticide formulation should offer advantages such as good water dispersibility, strong foliar affinity, sustained or controlled release of active ingredients, photostability and rain-fastness, minimal nontarget toxicity, use of nontoxic organic solvents, and degradable adjuvants. In line with this objective, we present green preparation of a colloidal pesticide formulation using optimized lysine-derived carbon dots (LysCDs)-modified CaCO₃ (LysCDs/CaCO₃) particles as the carrier and abamectin (Abm) as the active ingredient. The loading capacity of abamectin in this colloidal pesticide (LysCDs/CaCO₃/Abm) is 1.7 to 2.1 times higher than that of its counterpart (CaCO₃/Abm) prepared without LysCDs, which is attributed to the increased specific surface area and pore volume of LysCDs/CaCO₃ particles. Due to the acid-induced degradation of CaCO₃, the release of abamectin for LysCDs/CaCO₃/Abm is accelerated under weakly acidic conditions, which is accompanied by the release of Ca²⁺ ions and the fluorescence changes of LysCDs. The incorporation of LysCDs enhances the photostability and foliar adhesiveness of this colloidal pesticide, resulting in the highest degree of foliar retention when exposed to ultraviolet (UV) light or rainfall, compared to free-form abamectin and CaCO₃/Abm. This results in the best performance of pest control on <i>Plutella xylostella</i> for LysCDs/CaCO₃/Abm in both indoor and outdoor tests. Nontarget biocompatibility evaluations show that LysCDs/CaCO₃/Abm exhibits lower acute toxicity to zebrafish and earthworms than free-form abamectin. In addition, this colloidal pesticide is favored by the minimal residue of the adjuvant material after abamectin release, which is converted into harmless Ca²⁺ ions, CO₂, and LysCDs. Therefore, this work designs a safer and more effective colloidal pesticide formulation to deliver abamectin with minimal adjuvant residue, realizing its trajectory as basically “circular and green”.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"18 797 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic “Trojan Horse” Fibers Modulate Innate Immunity Cascades for Nerve Regeneration","authors":"Jie Wu, Jincheng Tang, Lichen Zhang, Wei Wang, Ziang Li, Liang Zhou, Xinzhao Jiang, Yiyang Huang, Qiangqiang Guo, Wenbo Wang, Zhouye Ding, Feng Cai, Kun Xi, Yong Gu, Liang Chen","doi":"10.1021/acsnano.4c12036","DOIUrl":"https://doi.org/10.1021/acsnano.4c12036","url":null,"abstract":"Neutrophil membrane vesicles (NMVs) have been successfully applied to control the inflammatory cascade after spinal cord injury (SCI) by acting as an inflammatory factor decoy to front-load the overall inflammation regulatory window; however, the mechanisms by which NMVs regulate macrophage phenotypic shifts as well as their outcomes have rarely been reported. In this study, we demonstrated the “efferocytosis-like” effect of NMVs endocytosed by macrophages, supplementing the TCA cycle intermediate metabolite α-KG by promoting glutamine metabolism, which in turn facilitates oxidative phosphorylation and inhibits the NF-κB signaling pathway to reprogram inflammatory macrophages to the pro-regenerative phenotype. Based on these findings, a “Trojan horse” composite fiber scaffold was constructed; this comprised a carboxylated poly-<span>l</span>-lactic acid shell encapsulated with NMVs and a core loaded with brain-derived neurotrophic factor to spatiotemporally modulate the inflammatory microenvironment by 39.23% and sustainably promote nerve regeneration by 85.67%. In vivo experiments further confirmed the effect of NMV-coated fiber scaffolds on the regulation of early innate immune inflammation and the continuous promotion of nerve regeneration. This study not only further unravels the mechanism of neutrophil membrane–macrophage interactions but also provides a strategy for coordinating inflammatory reprogramming and nerve regeneration following SCI.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"177 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}