生命科学最新文献

{"title":"Metabolism Interaction Between Bacillus cereus SESY and Brassica napus Contributes to Enhance Host Selenium Absorption and Accumulation.","authors":"Huan Zhang, Lianming Liang, Xiaoping Du, Guangyu Shi, Xu Wang, Yanni Tang, Zheng Lei, Yin Wang, Ceng Yi, Chengxiao Hu, Xiaohu Zhao","doi":"10.1111/pce.15278","DOIUrl":"https://doi.org/10.1111/pce.15278","url":null,"abstract":"<p><p>The use of beneficial bacteria to enhance selenium absorption in crops has been widely studied. However, it is unclear how the interaction between bacteria and plants affects selenium absorption in crops. Here, pot experiments and Murashige and Skoog medium (MS) experiments were performed. Transcriptomic analyses were used to reveal the interaction between Bacillus cereus SESY and Brassica napus. The results indicated that B. cereus SESY can significantly increase the biomass and selenium content of B. napus. The genes related to the colonization, IAA synthesis, and l-cysteine synthesis and metabolism of B. cereus SESY were significantly stimulated by B. napus through transcriptional regulation. Further verification results showed that l-cysteine increased selenium content in B. napus roots and shoots by 62.9% and 88.4%, respectively. B. cereus SESY and l-cysteine consistently regulated the relative expression level of genes involved in plant hormone, amino acid metabolism, selenium absorption, and Se enzymatic and nonenzymatic metabolic pathway of B. napus. These genes were significantly correlated with selenium content and biomass of B. napus (p < 0.05). Overall, IAA biosynthesis, and l-cysteine biosynthesis and metabolism in B. cereus SESY stimulated by interactions triggered molecular and metabolic responses of B. napus, underpinning host selenium absorption and accumulation.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular hydrogen positively influences root gravitropism involving auxin signaling and starch accumulation.","authors":"Yingying Zhang, Ziyu Liu, Huize Huang, Longna Li, Sheng Xu, Wenbiao Shen","doi":"10.1111/tpj.17151","DOIUrl":"https://doi.org/10.1111/tpj.17151","url":null,"abstract":"<p><p>Although geoscience of natural hydrogen (H₂), hydrogen-producing soil bacteria, and especially plant-based H₂, has been observed, it is not clear whether or how above H₂ resources influence root gravitropic responses. Here, pharmacological, genetic, molecular, and cell biological tools were applied to investigate how plant-based H₂ coordinates gravity responses in Arabidopsis roots. Since roots show higher H₂ production than shoots, exogenous H₂ supply was used to mimic this function. After H₂ supplementation, the asymmetric expression of the auxin-response reporter DR5 driven by auxin influx and efflux carriers, and thereafter positive root gravitropism were observed. These positive responses in root gravitropism were sensitive to auxin polar transport inhibitors, and importantly, the defective phenotypes observed in aux1-7, pin1, and pin2 mutants were not significantly altered by exogenous H₂. The observed starch accumulation was matched with the reprogramming gene expression linked to starch synthesis and degradation. Transgenic plants expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtii not only displayed higher endogenous H₂ concentrations, the inducible AUX1 gene expression and starch accumulation, but also showed pronounced root gravitropism. Collectively, above evidence preliminarily provides a framework for understanding the molecular basis of the possible functions of both plant/soil-based and nature H₂ in root architecture.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of lignin biosynthesis by GhCAD37 affects fiber quality and anther vitality in upland cotton.","authors":"Haipeng Li, Jinggong Guo, Kun Li, Yuwen Gao, Hang Li, Lu Long, Zongyan Chu, Yubei Du, Xulong Zhao, Bing Zhao, Chen Lan, José Ramón Botella, Xuebin Zhang, Kun-Peng Jia, Yuchen Miao","doi":"10.1111/tpj.17149","DOIUrl":"https://doi.org/10.1111/tpj.17149","url":null,"abstract":"<p><p>Cotton stands as a pillar in the textile industry due to its superior natural fibers. Lignin, a complex polymer synthesized from phenylalanine and deposited in mature cotton fibers, is believed to be essential for fiber quality, although the precise effects remain largely unclear. In this study, we characterized two ubiquitously expressed cinnamyl alcohol dehydrogenases (CAD), GhCAD37A and GhCAD37D (GhCAD37A/D), in Gossypium hirsutum. GhCAD37A/D possess CAD enzymatic activities, to catalyze the generation of monolignol products during lignin biosynthesis. Analysis of transgenic cotton knockout and overexpressing plants revealed that GhCAD37A/D are important regulators of fiber quality, positively impacting breaking strength but negatively affecting fiber length and elongation percentage by modulating lignin biosynthesis in fiber cells. Moreover, GhCAD37A/D are shown to modulate anther vitality and affect stem lodging trait in cotton by influencing lignin biosynthesis in the vascular bundles of anther and stem, respectively. Additionally, our study revealed that Ghcad37A/D knockout plants displayed red stem xylem, likely due to the overaccumulation of aldehyde intermediates in the phenylpropanoid metabolism pathway, as indicated by metabolomics analysis. Thus, our work illustrates that GhCAD37A/D are two important enzymes of lignin biosynthesis in different cotton organs, influencing fiber quality, anther vitality, and stem lodging.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Some Like It Hot: Differential Photosynthetic Performance and Recovery of English Walnut Accessions Under Emerging California Heat Waves.","authors":"Mina Momayyezi, Troy Williams, Peter Tolentino, Abby Hammermeister, Daniel A Kluepfel, Elisabeth J Forrestel, Andrew J McElrone","doi":"10.1111/pce.15273","DOIUrl":"https://doi.org/10.1111/pce.15273","url":null,"abstract":"<p><p>Heat waves (HWs) pose a significant threat to California agriculture, with potential adverse effects on crop photosynthetic capacity, quality and yield, all of which contribute to significant economic loss. Lack of heat-resilient cultivars puts perennial crop production under severe threat due to increasing HW frequency, duration and intensity. Currently, available walnut cultivars are highly sensitive to abiotic stress, and germplasm collections provide potential solutions via genotypes native to varied climates. We screened nine English walnut accessions (Juglans regia) for physiological heat stress resilience and recovery in the USDA-ARS National Clonal Germplasm over 2-years, and identified accessions with superior resilience to heat stress. Heat stress impacted photosynthetic capacity in most accessions, as evidenced by reductions in net (A_n) and maximum (A_max) assimilation rates, quantum efficiency of PSII, and changes in stomatal conductance (g_s). However, two accessions exhibited either higher or complete recovery post-irrigation. This aligns with the established practice of using irrigation to mitigate heat stress, as it improved recovery for several accessions, with A3 and A5 demonstrating the most resilience. One of these two superior accessions is native to one of the hottest and driest habitats of all studied accessions. These same accessions exhibited the highest A_n under non-stressed conditions and at higher temperatures of 35° to 45°C. Higher performance for A3 and A5 under HWs was associated with greater carboxylation rates, electron transport rates, and A_max. All accessions suffered significant declines in photosynthetic performance at 45°C, which were the ambient leaf temperatures approached during record-setting heat waves in California during September 2022.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Association Study Identifies the Serine/Threonine Kinase ClSIK1 for Low Nitrogen Tolerance in Watermelon Species.","authors":"Kejia Zhang, Qinrong Yang, Yongming Bo, Yimei Zhou, Nanqiao Liao, Xiaolong Lyu, Jinghua Yang, Zhongyuan Hu, Mingfang Zhang","doi":"10.1111/pce.15275","DOIUrl":"https://doi.org/10.1111/pce.15275","url":null,"abstract":"<p><p>Plants have evolved multiple complex mechanisms enabling them to adapt to low nitrogen (LN) stress via increased nitrogen use efficiency (NUE) as nitrogen deficiency in soil is a major factor limiting plant growth and development. However, the adaptive process and evolutionary roles of LN tolerance-related genes in plants remain largely unknown. In this study, we resequenced 191 watermelon accessions and examined their phenotypic differences related to LN tolerance. A major gene ClSIK1 encoding a serine/threonine protein kinase involved in the response to LN stress was identified on chromosome 11 using genome-wide association study and RNA-Seq analysis. According to a functional analysis, ClSIK1 overexpression can increase the root area, total biomass, NUE and LN tolerance by manipulating multiple nitrogen-metabolized genes. Interestingly, the desirable LN-tolerant haplotype ClSIK1^HapC was detected in only one wild relative (Citrullus mucosospermus) and likely gradually lost during watermelon domestication and improvement. This study clarified the regulatory effects of ClSIK1 on NUE and adaptations to LN stress, which also identifying valuable haplotypes-resolved gene variants for molecular design breeding of 'green' watermelon varieties highly tolerant to LN stress.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-Photoperiod Induces Floral Induction Involving Carbohydrate Metabolism and Regulation by VcCO3 in Greenhouse Blueberry.","authors":"Xin Feng, Xinliang Wu, Huiling Wu, Yang Li, Bingjie Zhou, Ying Jiang, Suilin Zhang, Jiali Wei, Shuchai Su, Zhixia Hou","doi":"10.1111/pce.15292","DOIUrl":"https://doi.org/10.1111/pce.15292","url":null,"abstract":"<p><p>Blueberry flower buds cultivated in greenhouses develop during both autumn and spring, with floral induction being a critical process for flowering, influenced by environmental factors. This study aimed to clarify the regulatory mechanisms governing floral induction in greenhouse blueberries, focusing on the similarities and differences in flower bud differentiation between the spring and autumn seasons. Understanding these mechanisms is pivotal for enhancing blueberry production. In this study, we analysed the phenotypic characteristics associated with flower bud differentiation and observed that short photoperiods markedly affect the induction process. Transcriptomic analyses revealed distinct major metabolic pathways activated in autumn compared to spring. Seasonal variations in carbohydrate metabolism were also noted, with sucrose hydrolysis being prominent in autumn and sucrose synthesis prevailing in spring. The interplay between circadian rhythms and photosynthesis appeared to facilitate the allocation of sugars for bud development. Subsequent investigations underscored the sensitivity of VcCO3 to variations in photoperiod. Predominantly localised in the nucleus, VcCO3 facilitated floral induction in response to short photoperiods by activating the expression of downstream genes, including VcFT, VcLFY, VcAP3, and VcSOC1. Furthermore, VcCO3 exhibits a close association with the sugar metabolism gene VcSUS, promoting increased sucrose concentrations.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil Microbiota Modulates Root Transcriptome With Divergent Effect on Maize Growth Under Low and High Phosphorus Inputs.","authors":"Chao Wang, Huanhuan Tai, Yinglong Chen, Zhiwen Zhai, Lin Zhang, Zitian Pu, Maolin Zhang, Chunjian Li, Zhihong Xie","doi":"10.1111/pce.15281","DOIUrl":"https://doi.org/10.1111/pce.15281","url":null,"abstract":"<p><p>Plant growth can be promoted by beneficial microorganisms, or inhibited by detrimental ones. Although the interaction process between a single microbial species and its host has been extensively studied, the growth and transcriptional response of the host to soil microbiota is poorly understood. We planted maize in natural or sterile soil collected from a long-term experimental site with two different soil phosphate (P) regimes. We examined the composition of microbial communities inhabiting root-associated niches in natural soil. In parallel, we determined the biomass, ionomes, and root transcriptome profiling of maize grown in natural or sterile soil. Soil microbiota could promote or inhibit different P starvation-responsive (PSR) genes, as well as induce several defense-related metabolic processes independently of external P levels. Soil microbiota accompanied by long-term application of P fertilizer induced lower intensity of PSR and defense responses, inhibiting maize growth. Under a low P regime, the PSR and defense responses were induced to a higher extent, promoting P absorption and growth. Our findings suggest a soil P-dependent effect of microbiota on maize growth by integrating PSR and defense responses and provide a more refined understanding of the interaction between root growth and soil microbiota.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Individual Versus Combined Effects of Warming, Elevated CO₂ and Drought on Grassland Water Uptake and Fine Root Traits.","authors":"Maud Tissink, Jesse Radolinski, David Reinthaler, Sarah Venier, Erich M Pötsch, Andreas Schaumberger, Michael Bahn","doi":"10.1111/pce.15274","DOIUrl":"https://doi.org/10.1111/pce.15274","url":null,"abstract":"<p><p>Increasing warming, atmospheric CO₂ and drought are expected to change the water dynamics of terrestrial ecosystems. Yet, limited knowledge exists about how the interactive effects of these factors will affect grassland water uptake, and whether adaptations in fine root production and traits will alter water uptake capacity. In a managed C₃ grassland, we tested the individual and combined effects of warming (+3°C), elevated CO₂ (eCO₂; +300 ppm) and drought on root water uptake (RWU) as well as on fine root production, trait adaptation, and fine root-to-shoot production ratios, and their relationships with RWU capacity. High temperatures, amplified by warming, exacerbated RWU reductions under drought, with negligible water-sparing effects from eCO₂. Drought, both under current and future (warming, eCO₂) climatic conditions, shifted RWU towards deeper soil layers. Overall, RWU capacity related positively to fine root production and specific root length (SRL), and negatively to mean root diameters. Warming effects on traits (reduced SRL, increased diameter) and the ratio of fine root-to-shoot production (increased) were offset by eCO₂. We conclude that under warmer future conditions, irrespective of shifts in water sourcing, it is particularly hot droughts that will lead to increasingly severe restrictions of grassland water dynamics.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The C₂H₂-type zinc finger transcription factor ZmDi19-7 regulates plant height and organ size by promoting cell size in maize.","authors":"Jinlei Dong, Zimeng Wang, Weina Si, Huan Xu, Zhen Zhang, Qiuyu Cao, Xinyuan Zhang, Hui Peng, Rongwei Mao, Haiyang Jiang, Beijiu Cheng, Xiaoyu Li, Longjiang Gu","doi":"10.1111/tpj.17139","DOIUrl":"https://doi.org/10.1111/tpj.17139","url":null,"abstract":"<p><p>The drought-induced protein 19 (Di19) gene family encodes a Cys2/His2 zinc-finger protein implicated in responses to diverse plant stressors. To date, potential roles of these proteins as transcription factors remain largely elusive in maize. Here, we show that ZmDi19-7 gene exerts pivotal functions in regulation of plant height and organ growth by modulating the cell size in maize. ZmDi19-7 physically interacts with ubiquitin receptor protein ZmDAR1b, which is indispensable in ubiquitination of ZmDi19-7 and affects its protein stability. Further genetic analysis demonstrated that ZmDAR1b act in a common pathway with ZmDi19-7 to regulate cell size in maize. ZmDi19-7, severing as a transcriptional factor, is significantly enriched in conserved DiBS element in the promoter region of ZmHSP22, ZmHSP18c, ZmSAUR25, ZmSAUR55, ZmSAUR7 and ZmXTH23 and orchestrates the expression of these genes involving in auxin-mediated cell expansion and protein processing in the endoplasmic reticulum. Thus, our findings demonstrate that ZmDi19-7 is an important newfound component of the ubiquitin-proteasome pathway in regulation of plant height and organ size in maize. These discoveries highlight potential targets for the genetic improvement of maize in the future.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Key R2R3-MYB Transcription Factor Activates Anthocyanin Biosynthesis and Leads to Leaf Reddening in Poplar Mutants.","authors":"Weinan Li, Qianqian Li, Jiahang Che, Jie Ren, Aike Wang, Jinhuan Chen","doi":"10.1111/pce.15276","DOIUrl":"https://doi.org/10.1111/pce.15276","url":null,"abstract":"<p><p>Colorful woody plants are highly valued for their ornamental qualities, and are commonly used in garden landscape design. We previously cultivated several ornamental poplar varieties from bud mutants of Populus sp. Linn. '2025' (ZL2025), each with different leaf colors. Based on transcriptome data from these varieties with varying anthocyanin pigmentation, we identified and named an R2R3-MYB gene, PdMYB113. The mRNA of PdMYB113 accumulated in the leaves of the red-leaf mutants 'QHY' and 'LHY', but barely expressed in the leaves of 'ZL2025'. The anthocyanin biosynthesis genes were upregulated, resulting in high levels of red anthocyanins (particularly Peonidin-3-O-rutinoside, Cyanidin-3-O-rutinoside, and Cyanidin-3-O-glucoside) in both OE-PdMYB113 tobacco and poplar plants. This upregulation caused a color change in the tissues from green to red or dark purple. Yeast one-hybrid and luciferase assays demonstrated that PdMYB113 activates the expression of anthocyanin biosynthesis genes, including the early anthocyanin biosynthetic gene PdCHS and the late anthocynin biosynthetic gene PdANS. Consequently, PdMYB113 is identified as a key regulator of red coloration in poplar. Additionally, PdMYB113 does not dwarf transgenic plants under normal lighting conditions. This study elucidates the regulatory mechanisms of color change in ZL2025 and highlights a crucial gene for breeding new varieties of woody plants.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}