生命科学最新文献

{"title":"Phosphorylation in two acts: Marchantia phototropin undergoes sequential cis- and trans-autophosphorylation","authors":"Martin Balcerowicz","doi":"10.1111/tpj.70072","DOIUrl":"https://doi.org/10.1111/tpj.70072","url":null,"abstract":"<p>Plants optimise photosynthetic performance and protect themselves from light-induced damage by sensing the intensity, periodicity, composition and direction of light. Consequently, plants have evolved several sets of photoreceptors to monitor their light environment. Phototropins are UV-A and blue light receptors that regulate responses such as phototropism, leaf flattening, chloroplast positioning and stomatal opening (Hart & Gardner, <span>2021</span>). Among these, light-induced chloroplast movement is one of the most conserved processes and can be observed not only in seed plants, but also in green algae, mosses and liverworts. Under low light conditions, chloroplasts accumulate at cell walls and orient perpendicular to the incoming light, maximising photosynthetic efficiency. Strong light intensities instead elicit an avoidance response where chloroplasts reorient parallel to the incoming light. This is generally thought of as a photoprotective mechanism, avoiding light-induced damage to the photosynthetic apparatus, but also allowing light to penetrate into deeper tissues (Łabuz et al., <span>2022</span>).</p><p>Phototropins contain two light-sensing LOV (Light, Oxygen, or Voltage-sensing) domains and a C-terminal serine/threonine kinase domain. In darkness, the LOV2 domain represses the kinase activity. Upon light absorption, this repression is relieved, triggering autophosphorylation of the kinase domain at multiple serine and threonine residues – an essential step in phototropin signalling (Hart & Gardner, <span>2021</span>). Phototropins form dimers, suggesting that autophosphorylation can occur both in <i>cis</i> (by the molecule's own kinase domain) and in <i>trans</i> (by its dimer partner). Arabidopsis phototropin 1 (Atphot1) was shown to undergo trans-autophosphorylation (Kaiserli et al., <span>2009</span>; Petersen et al., <span>2017</span>), but such mechanistic insight remains scarce for phototropins of other species.</p><p>Yutaka Kodama got interested in phototropins because of their major role in chloroplast movement. He worked on chloroplast biology during his postdoc in the lab of Masamitsu Wada at the National Institute for Biology and Kyushu University, after which he gained further expertise in bioimaging with Chang-Deng Hu at Purdue University. He started his own lab at Utsunomiya University in 2011, where his research focuses on organelle biology and developing innovative imaging technology. Studying chloroplast dynamics in response to external stimuli led him to explore phototropin function. Minoru Noguchi, currently a PhD candidate in the group, is particularly interested in phototropin signalling pathways and has been studying phototropin function in the liverwort <i>Marchantia polymorpha</i> (Marchantia).</p><p>Marchantia harbours a single copy phototropin gene (<i>MpPHOT</i>) that mediates chloroplast accumulation and avoidance responses under weak and strong light, respectively (Komatsu et al., <span>20","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indigenous student engagement in science: a case study addressing the lack of diversity and equity in biomedical science and pharmacy research for Aboriginal and Torres Strait islander people.","authors":"Wukul Yabang, Elizabeth E Manning, Karen E Mate, Saije K Endacott, Guy Jm Cameron","doi":"10.1111/imcb.70007","DOIUrl":"https://doi.org/10.1111/imcb.70007","url":null,"abstract":"<p><p>Indigenous representation in Australian biomedical science and pharmacy research remains limited due to systemic barriers, historical marginalization and culturally inappropriate educational frameworks. This article outlines a case study of initiatives at the University of Newcastle (UoN) aimed at addressing these inequities. Central to this effort is the establishment of the Indigenous Student Engagement Committee, which promotes Indigenous participation across all academic stages. Working in conjunction with key programs, including culturally embedded pathways such as the Yapug and Miroma Bunbilla programs, undergraduate and postgraduate research fellowships, and culturally inclusive curricula, demonstrate UoN's commitment to fostering a robust pipeline for Indigenous researchers. The UoN's initiatives are grounded in collaboration with local Aboriginal communities, ensuring relevance and cultural safety. Early engagement programs with primary and secondary schools, supported by partnerships with the Wollotuka Institute, create pathways that demystify science and higher education. Hands-on experiences, such as laboratory work placements, enhance accessibility and interest among Indigenous students. At the tertiary level, efforts focus on indigenizing curricula and providing dedicated spaces and mentorship that nurture academic success and cultural connection. The article also highlights challenges, including the rigidity of traditional funding models, the discomfort of non-Indigenous staff in this space, and the need for flexible, inclusive recruitment practices. Recommendations for addressing these barriers include ongoing cultural capability training, mentorship programs and tailored funding constructs that accommodate community commitments. By outlining the UoN's comprehensive, culturally responsive strategies, this case study offers a model for increasing Indigenous engagement in biomedical sciences. It underscores the importance of systemic change, collaboration and sustained investment in creating equitable pathways for Indigenous students and researchers, ultimately contributing to a more inclusive academic and research environment in Australia.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A trainee-led approach to tackling gender inequity in immunology.","authors":"Hannah Driks, Letitia Yang, Sara Misiukiewicz, Gabriella Reeder, Jing-Yi Chung","doi":"10.1111/imcb.70008","DOIUrl":"https://doi.org/10.1111/imcb.70008","url":null,"abstract":"<p><p>Gender inequities persist in science, with women encountering significant barriers at various career stages, particularly in fields such as Immunology. This article highlights the work of Immunologists for Gender Equity (IgEquity), a trainee-led organization within the ImmunoX Program at the University of California, San Francisco (UCSF), which is committed to addressing these disparities. Through initiatives focused on community building, mentorship, outreach and advocacy, IgEquity seeks to advance gender equity in academia. We emphasize the critical role that trainee-led organizations can play in driving change and underscore the importance of institutional support in creating lasting, systemic progress toward gender equity in the scientific community.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trade-Offs Between Growth, Longevity, and Storage Carbohydrates in Herbs and Shrubs: Evidence for Active Carbon Allocation Strategies.","authors":"Kenz Raouf Samraoui, Adam Klimeš, Veronika Jandová, Nela Altmanová, Jan Altman, Miroslav Dvorský, Vojtech Lanta, Klára Řeháková, Adam Taylor Ruka, Pavel Fibich, Pierre Liancourt, Jiří Doležal","doi":"10.1111/pce.15444","DOIUrl":"https://doi.org/10.1111/pce.15444","url":null,"abstract":"<p><p>Plants store nonstructural carbohydrates (NSCs) like starch, fructans and soluble sugars to support metabolism, stress tolerance and defence during low photosynthesis, ultimately influencing their growth and longevity. However, the relationship between NSC composition and growth or persistence in wild plants remains unclear. This study explores trade-offs between growth, longevity and NSCs in 201 plant species across diverse climates in the Western USA, spanning 500-4300 m in elevation and 80-1000 mm in precipitation. Annual growth rates and plant ages were derived from the ring widths of semidesert, steppe and alpine herbs and shrubs, along with NSC profiles in their roots and rhizomes. Results showed an inverse relationship between growth and age, with total NSC, starch and fructan levels negatively correlated with growth, supporting the growth-longevity and growth-storage trade-off hypotheses. Conversely, higher growth rates were linked to soluble sugars, suggesting that climate-driven growth limitations alone do not explain increased NSCs. Fructans were positively associated with longevity, especially in long-lived desert shrubs and alpine herbs, underscoring NSCs' active role in survival strategies. These findings challenge the carbon surplus hypothesis, suggesting that plants actively use specific NSCs to balance growth and persistence, with energy-rich sugars promoting growth and osmoprotective fructans enhancing longevity.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of methyltransferase 1-dependent DNA methylation in affecting maize kernel development","authors":"Xiaosong Li,&nbsp;Xuqing Tong,&nbsp;Bin Wang,&nbsp;Menglin Pu,&nbsp;Guangming Zheng,&nbsp;Bohui Wang,&nbsp;Jun Li,&nbsp;Xiaofei He,&nbsp;Zhilong Liu,&nbsp;Haiping Ding,&nbsp;Zhiming Zhang,&nbsp;Xiansheng Zhang,&nbsp;Chao Zhou,&nbsp;Xiangyu Zhao","doi":"10.1111/tpj.17250","DOIUrl":"https://doi.org/10.1111/tpj.17250","url":null,"abstract":"<div>\u0000 \u0000 <p>DNA methylation plays an essential role in plant growth and development, however, its specific influence on maize kernel development remains uncertain. In this study, we investigated the gene responsible for the maize kernel mutant <i>smk313</i> and identified it as the DNA methyltransferase ZmMET1. The <i>smk313</i> mutants displayed a distinct small kernel phenotype and exhibited developmental abnormalities in the basal endosperm transfer layer (BETL), the endosperm adjacent to the scutellum cell (EAS), and the starchy endosperm cells (SEs). Compared with that of the wild-type (WT), we found that the mutants had lower CG methylation density across the whole genome through whole genome methylation sequencing (WGBS), and there were many accessible chromatin regions (ACRs) through assay for targeting accessible chromatin with high-throughout sequencing (ATAC-seq). Combining these findings with the transcriptome analysis revealed a cascade of effects caused by the loss of <i>ZmMET1</i> function. This deficiency leads to alterations in genomic methylation and chromatin accessibility, which in turn influences the expression of genes related to starch and protein synthesis, as well as material transport processes. These alterations were consistent with the delayed development and dysplasia observed in EAS and BETLs of <i>smk313</i> kernels. Consequently, our investigation emphasizes the vital role of <i>ZmMET1</i> in maize seed development.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetic induction and sunfleck responses of three understory tree species in forests of the Mid-Atlantic U.S","authors":"Jessica L. Schedlbauer,&nbsp;Sarah Paynter","doi":"10.1007/s00468-025-02611-3","DOIUrl":"10.1007/s00468-025-02611-3","url":null,"abstract":"<div><h3>Key message</h3><p>Photosynthetic induction was rapid in three shade-tolerant understory saplings, and surprisingly, native and non-native <i>Acer</i> species gained induction more quickly when exposed to sequential lightflecks, relative to constant saturating light.</p><h3>Abstract</h3><p>Growing regeneration mismatch between forest canopy and understory environments is evident in broadleaf forests of the eastern U.S., leading to novel understory forest composition. Three co-occurring shade-tolerant tree species in forest understories of southeastern Pennsylvania are the natives <i>Acer rubrum</i> and <i>Fagus grandifolia</i> and the non-native <i>Acer platanoides</i>. Physiological attributes contributing to these species’ relative success in the understory’s dynamic light environment are poorly characterized. Three saplings per species from three sites were sampled to examine photosynthetic induction and lightfleck responses. Predictors of photosynthesis (A) and stomatal conductance (g_s) during lightflecks were also examined. The rate and magnitude of photosynthetic induction were similar among species, with all gaining induction rapidly, as expected for shade-tolerant species. However, physiological strategies supporting induction responses varied by species, as <i>A. rubrum</i> had more responsive stomata and significantly lower stomatal limitation during induction, as well as significantly higher A/g_s when fully induced. Surprisingly, lightfleck responses differed from induction responses for the two <i>Acer</i> species, both of which exhibited significantly greater cumulative carbon assimilation than when exposed to constant saturating light, particularly during short-duration lightflecks. Leaf nitrogen and g_s were significant predictors of lightfleck responses, while species identity was not. Although induction gain under saturating light was rapid for all species, variable lightfleck responses favoring the <i>Acer</i> species provide an advantage given the intermittent nature of understory light availability. As eastern broadleaf forest composition continues to change in response to diverse pressures, the <i>Acer</i> species are well-positioned to persist.</p></div>","PeriodicalId":805,"journal":{"name":"Trees","volume":"39 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GIGANTEA Is Required for Robust Circadian Rhythms in Wheat.","authors":"Laura J Taylor, Gareth Steed, Gabriela Pingarron-Cardenas, Lukas Wittern, Matthew A Hannah, Alex A R Webb","doi":"10.1111/pce.15447","DOIUrl":"https://doi.org/10.1111/pce.15447","url":null,"abstract":"<p><p>GIGANTEA (GI) is a plant-specific protein that functions in many physiological processes and signalling networks. In Arabidopsis, GI has a central role in circadian oscillators regulating the abundance of ZEITLUPE and TIMING OF CAB EXPRESSION 1 proteins and is essential for photoperiodic regulation of flowering. We have investigated how ortholgues of this component of Arabidopsis circadian oscillators contribute to circadian rhythms and yield traits, including heading (flowering) in wheat. We find that GI is a core component of wheat circadian oscillators that is necessary to maintain robust oscillations in chlorophyll fluorescence and circadian oscillator transcript abundance. The predicted lack of functional GI results in later flowering of wheat in both long days and short days in controlled environment conditions. Our results support and extend previous work, which suggests that the pathways by which photoperiodism regulates flowering are not fully conserved between Arabidopsis and wheat. Understanding the molecular basis for photoperiodism in wheat is important for breeders looking to manipulate flowering time and develop new elite, high-yielding cultivars.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notch dependent chromatin remodeling enables Gata3 binding and drives lineage specific CD8⁺ T cell function.","authors":"Jessie O'Hara, Pushkar Dakle, Michelle Ly Thai Nguyen, Adele Barugahare, Taylah J Bennett, Vibha Av Udupa, Nicholas Murray, Gemma Schlegel, Constantine Kapouleas, Jasmine Li, Stephen J Turner, Brendan E Russ","doi":"10.1111/imcb.70002","DOIUrl":"https://doi.org/10.1111/imcb.70002","url":null,"abstract":"<p><p>Activation of CD8⁺ T cells enable them to control virus infections and tumors. This process involves the differentiation of naïve CD8⁺ T cells into effector and memory states, driven by specific transcription factors (TFs). Previously, we have shown that Granzyme A (Gzma) induction in activated CD8⁺ T cells depends on Gata3 and the establishment of a permissive chromatin landscape at the Gzma locus. Interestingly, Gzma expression is independent of IL-4 signaling, which typically upregulates Gata3 in CD4⁺ T cells, suggesting an alternative pathway for Gata3 induction. Here we demonstrate that Notch signals during CD8⁺ T cell activation promote Gzma expression. Inhibition of Notch signaling or loss of the Notch transactivator Rbp-j leads to reduced Gzma expression, with transcriptionally repressive chromatin at the Gzma locus. The genome targets of Gata3 differ in effector CD8⁺ T cells activated with IL-4 compared with those activated with Notch signals or isolated after IAV infection. This indicates that the signals received during CD8⁺ T cell activation can alter the chromatin landscape, affecting Gata3 function. Furthermore, Gata3 deficiency results in reduced IAV-specific CD8⁺ T cell responses and decreased Gzma expression, although the Gzma locus maintains a permissive chromatin landscape. These findings suggest that Notch signals received by virus-specific CD8⁺ T cells prepare the chromatin landscape for Gata3 binding to CD8⁺ lineage-specific gene loci, promoting effective CD8⁺ T cell immunity.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Both Jasmonic Acid- and Abscisic Acid-Mediated Signalling Pathways Regulate the Ovicidal Defence of Plants Against Phloem-Feeding Insects.","authors":"Qing Gao, Nuo Jin, Zhifan Shen, Jingran Guo, Haiping Lu, Shanjie Han, Wenhan Xiao, Jing Lu, Yonggen Lou","doi":"10.1111/pce.15445","DOIUrl":"https://doi.org/10.1111/pce.15445","url":null,"abstract":"<p><p>Plants perceive signals associated with herbivore eggs and in response initiate ovicidal defence. However, which phytohormone pathways regulate this defence and which defensive compounds dominate it remains largely unknown. Here, we found that the hatching rate of eggs of white-backed planthopper (WBPH) Sogatella furcifera was significantly lower on a japonica rice variety P81 than an indica rice variety NB44. When infested by WBPH, P81 plants showed higher jasmonic acid (JA) and abscisic acid (ABA) responses than did NB44 plants; moreover, P81 plants produced the ovicidal compound benzyl benzoate and exhibited higher levels of some flavonoids, phenolamides, and volatiles than were found in NB44 plants. Impairing the ABA-signalling pathway, especially the JA-signalling pathway in P81 plants enhanced the survival of WBPH eggs. Decreasing levels of some flavonoids and phenolamides in P81 plants promoted WBPH egg survival. In vitro bioassays revealed that both naringenin and sakuranetin promote the ovicidal effect of benzyl benzoate on WBPH. The results demonstrate that JA- and ABA-signalling pathways jointly regulate the rice ovicidal defence against WBPH, and that benzyl benzoate, as well as some other compounds, such as naringenin and sakuranetin, contribute to the mortality of WBPH eggs.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moso bamboo sucrose synthase gene SUS3 promotes earlier germination of tiller buds in Dendrocalamus brandisii ‘Manxie Tianzhu’","authors":"Weijie Sun,&nbsp;Muthusamy Ramakrishnan,&nbsp;Long-Hai Zou,&nbsp;Kai-Li Wang,&nbsp;Xiaohong Zhou,&nbsp;Mingbing Zhou","doi":"10.1007/s00468-025-02612-2","DOIUrl":"10.1007/s00468-025-02612-2","url":null,"abstract":"<div><h3>Key message</h3><p><b>Transient overexpression of </b><b><i>PeSUS3</i></b> <b>using a</b> <b><i>BaMV</i></b> <b>vector in</b><b><i> D. brandisii</i></b> <b>‘Manxie Tianzhu’ enhanced tillering bud germination and growth, suggesting a crucial role for sucrose metabolism in bamboo development.</b></p><h3>Abstract</h3><p>Bamboo shoots are highly sought after in China for their nutritional value. <i>Dendrocalamus brandisii</i> ‘Manxie Tianzhu’ (Manxie Sweet Dragon Bamboo) faces supply challenges due to limited distribution, necessitating faster growing, higher yielding varieties. The sucrose synthase gene, particularly <i>PeSUS3</i>, is vital for sucrose metabolism, nutrient transport, and regulating dormancy release and bud germination, yet its role in bamboo shoot production is not well understood. This study used a bamboo mosaic virus vector (BaMV) to overexpress <i>PeSUS3</i> in <i>D. brandisii</i> ‘Manxie Tianzhu’ seedlings. <i>PeSUS3</i> was cloned into the BaMV-P19-EGFP-2 vector, introduced into <i>Agrobacterium tumefaciens</i>, and used to infect tobacco leaves. The resulting virus was mechanically inoculated into the bamboo seedlings. Analysis revealed that <i>PeSUS3</i> overexpression increased the number of tiller buds and accelerated germination by 1–2 days compared to controls. Despite the transient overexpression of <i>PeSUS3</i>, the increased sucrose content in the overexpressing plants suggests a role for sucrose metabolism in these processes. These findings emphasize the importance of the transient overexpression of <i>PeSUS3</i> in promoting tillering bud germination and growth in <i>D. brandisii</i> ‘Manxie Tianzhu’, providing a foundation for future research on its function and mechanisms.</p></div>","PeriodicalId":805,"journal":{"name":"Trees","volume":"39 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}