Immunology & Cell Biology最新文献

ILC1 as critical gatekeepers in autoimmune kidney damage.

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-12-06 DOI: 10.1111/imcb.12842

Cyril Seillet, Le Xiong

引用次数: 0

RNAi library screening reveals Gβ1, Casein Kinase 2 and ICAP-1 as novel regulators of LFA-1-mediated T cell polarity and migration. RNAi 文库筛选发现 Gβ1、酪蛋白激酶 2 和 ICAP-1 是 LFA-1 介导的 T 细胞极性和迁移的新型调控因子。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-11-28 DOI: 10.1111/imcb.12838

Antje Haap-Hoff, Michael Freeley, Eugene Dempsey, Dara Dunican, Emily Bennett, Denise Triglia, Joanna Skubis-Zegadlo, Anthony Mitchell Davies, Dermot Kelleher, Aideen Long

{"title":"RNAi library screening reveals Gβ1, Casein Kinase 2 and ICAP-1 as novel regulators of LFA-1-mediated T cell polarity and migration.","authors":"Antje Haap-Hoff, Michael Freeley, Eugene Dempsey, Dara Dunican, Emily Bennett, Denise Triglia, Joanna Skubis-Zegadlo, Anthony Mitchell Davies, Dermot Kelleher, Aideen Long","doi":"10.1111/imcb.12838","DOIUrl":"https://doi.org/10.1111/imcb.12838","url":null,"abstract":"The αLβ2 integrin LFA-1 plays a key role in T-cell adhesion to the endothelial vasculature and migration into both secondary lymphoid organs and peripheral tissues via interactions with its target protein ICAM-1, but the pathways that regulate LFA-1-mediated T-cell polarity and migration are not fully understood. In this study we screened two RNAi libraries targeting G protein-coupled receptors (GPCR)/GPCR-associated proteins and kinases in a HuT 78 T cell line model of LFA-1-stimulated T-cell migration. Based on staining of the actin cytoskeleton, multiple parameters to measure cell morphology were used to assess the contribution of 1109 genes to LFA-1-mediated T-cell polarity and migration. These RNAi screens identified a number of both novel and previously identified genes that either increased or decreased the polarity and migratory capacity of these cells. Following multiparametric analysis, hierarchical clustering and pathway analysis, three of these genes were characterized in further detail using primary human T cells, revealing novel roles for the heterotrimeric G protein subunit Gβ1 and Casein Kinase 2 in LFA-1-mediated T-cell polarity and migration in vitro. Our studies also highlighted a new role for ICAP-1, an adaptor protein previously described to be associated with β1 integrins, in β2 integrin LFA-1-directed migration in T cells. Knockdown of ICAP-1 expression in primary T cells revealed a role in cell polarity, cell velocity and transmigration towards SDF-1 for this adaptor protein. This study therefore uncovers new roles for GPCR/GPCR-associated proteins and kinases in T-cell migration and provides potential novel targets for modulation of the T-cell immune response.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antigen presentation of post-translationally modified peptides in major histocompatibility complexes.

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-11-28 DOI: 10.1111/imcb.12839

Alexine S de Wit, Frans Bianchi, Geert van den Bogaart

{"title":"Antigen presentation of post-translationally modified peptides in major histocompatibility complexes.","authors":"Alexine S de Wit, Frans Bianchi, Geert van den Bogaart","doi":"10.1111/imcb.12839","DOIUrl":"https://doi.org/10.1111/imcb.12839","url":null,"abstract":"T cells of the adaptive immune system recognize pathogens and malignantly transformed cells through a process called antigen presentation. During this process, peptides are displayed on major histocompatibility complex (MHC) class I and II molecules. Self-reactive T cells are typically removed or suppressed during T-cell development and through peripheral tolerance mechanisms, ensuring that only T cells recognizing peptides that are either absent or present in low abundance under normal conditions remain. This selective process allows T cells to respond to peptides derived from foreign proteins while ignoring those from self-proteins. However, T cells can also respond to peptides derived from proteins that have undergone post-translational modifications (PTMs). Over 200 different PTMs have been described, and while they are essential for protein function, localization and stability, their dysregulation is often associated with disease conditions. PTMs can affect the proteolytic processing of proteins and prevent MHC binding, thereby changing the repertoire of peptides presented on MHC molecules. However, it is also increasingly evident that many peptides presented on MHC molecules carry PTMs, which can alter their immunogenicity. As a result, the presentation of post-translationally modified peptides by MHC molecules plays a significant role in various diseases, as well as autoimmune disorders and allergies. This review will provide an overview of the impact of PTMs on antigen presentation and their implications for immune recognition and disease.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anyone can cook, but only the fearless can be a great chef. 人人都会烹饪，但只有无所畏惧的人才能成为大厨。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-11-28 DOI: 10.1111/imcb.12841

Eduardo J Villablanca

引用次数: 0

The future of scientific labs: how we are making our research more sustainable. 科学实验室的未来：我们如何使我们的研究更具可持续性。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-11-27 DOI: 10.1111/imcb.12840

Ebony A Monson, Stephanie Rutter, Christopher C Reimann, Andrea Bueno-Pedraz, Caitlin Vella, Xavier G Pearce, Jennifer L Wood, Kerry V Fanson

引用次数: 0

Prenatal Skin Cell Atlas reveals macrophages' role beyond immunity. 产前皮肤细胞图谱揭示了巨噬细胞在免疫之外的作用。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-11-17 DOI: 10.1111/imcb.12837

Céline Pattaroni

引用次数: 0

The journey of young scientists in Brazil: challenges and perspectives. 巴西青年科学家的历程：挑战与展望。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-11-09 DOI: 10.1111/imcb.12835

Fabiana Corsi-Zuelli

引用次数: 0

When academia met industry: working toward a needle-free vaccination future in the sunshine state. 当学术界遇到工业界：在阳光之州努力实现无针疫苗接种的未来。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-11-09 DOI: 10.1111/imcb.12836

Arti Medhavy, Alex Johnston, Imogen Bermingham, Danielle I Stanisic

引用次数: 0

Choose your own T-cell fate: creation of a narrative-based, decision-making activity to engage students in immunology. 选择自己的 T 细胞命运：创建基于叙事的决策活动，让学生参与免疫学。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-11-05 DOI: 10.1111/imcb.12833

Helen E Ritchie, Gareth Denyer, Kylie E Webster

{"title":"Choose your own T-cell fate: creation of a narrative-based, decision-making activity to engage students in immunology.","authors":"Helen E Ritchie, Gareth Denyer, Kylie E Webster","doi":"10.1111/imcb.12833","DOIUrl":"https://doi.org/10.1111/imcb.12833","url":null,"abstract":"Undergraduate courses in immunology are content-heavy and combined with a new, complex vocabulary, can be an overwhelming subject for students. In-class active learning approaches have been found to improve understanding of difficult concepts in science, technology, engineering and mathematics (STEM) disciplines; however, many undergraduate courses maintain a high dependence on lecture-style teaching because of time constraints, content demands and student resistance. We designed an online, out-of-class activity, the \"Life and Death of a T cell\", to complement a lecture on a complex immunological concept, T-cell development. Inspired by the \"Choose Your Own Adventure\" children's books, a fictional narrative was created in which students assume the role of a cell with a dream of becoming a helper T cell. Decision-making scenarios then prompt students to draw on their knowledge from the lecture to successfully navigate the steps of T-cell development. The activity was built on two platforms, Google Forms and H5P (HTML 5 Package), both of which are readily accessible and allow the inclusion of branching logic and the creation of a decision tree-based activity. An anonymous survey revealed that students found this interactive approach enjoyable, and their perceived understanding of the content significantly increased. Students appreciated the inclusion of a novel learning resource, with requests for similar activities to be developed for other immunological concepts. In conclusion, we developed a narrative-based, decision-making activity to complement a lecture on T-cell development. As an out-of-class activity, this style of learning approach can potentially capitalize on the benefits of active learning, while also overcoming barriers of student resistance.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of clonally expanded γδ T-cell populations during CAR-T cell therapy. 识别 CAR-T 细胞疗法中克隆扩增的 γδ T 细胞群。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-11-05 DOI: 10.1111/imcb.12834

Arman Safavi, Jerome Samir, Mandeep Singh, Martina Bonomi, Raymond Yip Louie, Kenneth Micklethwaite, Fabio Luciani

{"title":"Identification of clonally expanded γδ T-cell populations during CAR-T cell therapy.","authors":"Arman Safavi, Jerome Samir, Mandeep Singh, Martina Bonomi, Raymond Yip Louie, Kenneth Micklethwaite, Fabio Luciani","doi":"10.1111/imcb.12834","DOIUrl":"https://doi.org/10.1111/imcb.12834","url":null,"abstract":"Anti-CD19 Chimeric Antigen Receptor (CAR)-T cell therapies have shown promise for treating B cell malignancies, but the clinical outcome is influenced by both the CAR-T product and the patient's immune system. The role of γδ T cells in the context of CAR-T cell therapy remains poorly understood. This study investigates the transcriptional heterogeneity, clonal expansion and dynamics of γδ T cells in patients undergoing anti-CD19 CAR-T cell therapy. Longitudinal single cell multi-omics analysis was performed on γδ T cells from four patients receiving anti-CD19 CAR-T cell therapy. Single cell RNA-seq, antibody-based protein profiling (AbSeq) and full-length TCRγδ sequences revealed clonally expanded populations displaying plasticity in T cell differentiation, and temporal dynamics of large clones, suggesting ongoing expansion and differentiation. Clonally expanded γδ T cells had heterogeneous gene expression profiles, occupying seven transcriptionally distinct clusters. Analysis of chemokine markers indicated cluster-specific homing tendencies of circulating γδ T cells to peripheral tissues. We found unexpectedly high frequencies of Vδ1 and Vδ3 cells in the blood with distinct gene and protein expression profiles. This analysis provides insights into the dynamic and heterogeneous nature of γδ T cells following anti-CD19 CAR-T cell therapy, contributing valuable information for optimizing CAR-T cell therapies in B cell malignancies.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0