Rajamanikandan Ramar, Sasikumar Kandasamy, Heongkyu Ju
{"title":"Emerging 2D MXene-conjugated nanoarchitectures for nanozymes: Current advances and future frontiers in biochemical sensing technologies","authors":"Rajamanikandan Ramar, Sasikumar Kandasamy, Heongkyu Ju","doi":"10.1039/d5en00710k","DOIUrl":"https://doi.org/10.1039/d5en00710k","url":null,"abstract":"Nanozymes, nanostructured materials with exceptional catalytic performance, are recognized for their unique benefits over natural enzymes, including notably low fabrication costs and high chemical stability. Emerging as a captivating frontier in nanozyme research, MXene-based composite material nanozymes (MXzymes) have sparked intense research interests owing to their unique compositions and structural features, which can be engineered to unlock their enzyme-mimicking catalytic prowess. This approach opens groundbreaking opportunities for detecting biologically significant, food-related, and environmentally crucial analytes. This review highlights the innovative fabrication methods of MXzymes, focusing on their diverse nanozyme activity types reported thus far, including peroxidase-like and oxidase-like functionalities, while unveiling the underlying catalytic mechanisms in detail. In addition, recent pioneering breakthroughs in MXzymes are comprehensively reviewed, including catalytic signal amplification by MXzymes, which can function as cutting-edge sensing platforms as evidenced by colorimetric, smartphone-based, chemiluminescent, electrochemical, and surface-enhanced Raman scattering-based analyses. Finally, the promising potential applications of MXzymes and fundamental challenges associated with their scalability, stability, and biocompatibility for MXzymes-based chemical/biomedical sensors are addressed.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"99 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuntian Shi, Kangping Jiao, Li'ang Li, Wenbo Guo, Mohamed H. Abo-Raya, Jae-Seong Lee, Rim EL Amouri, Menghong Hu, Youji Wang
{"title":"The stealthy journey of nanoplastics in bivalves: accumulation dynamics and toxic burden","authors":"Yuntian Shi, Kangping Jiao, Li'ang Li, Wenbo Guo, Mohamed H. Abo-Raya, Jae-Seong Lee, Rim EL Amouri, Menghong Hu, Youji Wang","doi":"10.1039/d5en00673b","DOIUrl":"https://doi.org/10.1039/d5en00673b","url":null,"abstract":"The strong filter-feeding capacity of bivalves makes them more prone to accumulating nanoplastic particles from their environment, posing a threat to aquaculture and food safety. Despite their inconspicuous size, nanoplastics embark on a stealthy invasion through bivalve tissues, evading conventional detection. Reliable detection methods for nanoplastics are essential for risk assessment. This paper provides a comprehensive review of nanoplastic detection techniques in biological tissues and suggests improvements in <em>in situ</em> detection and AI-based recognition methods. These advancements are critical to unveiling the hidden pathways of nanoplastics in biological systems. Next, we summarize the endocytic mechanisms and bioaccumulation patterns of nanoplastics based on particle size classification and realistic environmental scenarios, identifying gills and hepatopancreas as primary accumulation targets. This dynamic accumulation process highlights how nanoplastics progressively infiltrate key organs, escalating their toxic burden. Additionally, this paper offers a thorough overview of the pathways through which nanoplastics breach biological barriers and trigger cascading reactions, from cellular stress to organelle dysfunction, tissue damage, and ultimately organismal consequences. These cascading effects underscore the insidious yet pervasive toxic burden imposed by nanoplastics. Finally, this review identifies key research gaps, including the synergistic or inhibitory effects of coexisting marine pollutants on bivalve bioaccumulation and the unclear pathways and efficiency of nanoplastic accumulation in filter-feeding bivalves under eco-corona regulation. Unraveling these uncertainties is vital to mapping the full journey of nanoplastics and mitigating their ecological toll. This review aims to enhance the understanding of nanoplastic–bivalve interactions and guide mitigation strategies for their ecological effects.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"72 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fe2O3 nanoparticles are absorbed via the CLEC4E-mediated endocytosis in the intestine and via the LDLR/TFR1-mediated endocytosis in the liver of yellow catfish Pelteobagrus fulvidraco","authors":"Xiao-Lei Wei, An-Gen Yu, Zhi-Bo Liu, Kostas Pantopoulos, Peng-Cheng Xu, Hua Zheng, Zhi Luo","doi":"10.1039/d5en00270b","DOIUrl":"https://doi.org/10.1039/d5en00270b","url":null,"abstract":"The uptake of nanoparticles (NPs) in the intestine and liver is influenced by their biological milieu. However, the role of biomolecular corona in governing NPs uptake remained elusive. Here, we deciphered the composition of biomolecular corona on Fe2O3 NPs’ surface in the intestine. We identified a membrane protein, C-type lectin domain family 4E-like (CLEC4E), as a critical contributor to intestinal Fe2O3 NPs uptake. Mechanistically, CLEC4E mediated the internalization of Fe2O3 NPs in a clathrin- and caveolae-independent endocytic pathway. Additionally, we characterized the composition of serum protein corona on Fe2O3 NPs’ surface, and identified key ligands (apolipoprotein Eb, complement C8 alpha chain, complement C8 beta chain, apolipoprotein B-100, and serotransferrin-2 isoform X4) related to endocytosis. 69% and 21% of Fe2O3 NPs biodistribution among the six tested tissues could be explained by low-density lipoprotein receptor (LDLR) and transferrin receptor 1 (TFR1) expression, respectively. Mechanistically, LDLR and TFR1 mediated the internalization of Fe2O3 NPs, which was via clathrin-dependent endocytosis. For the first time, our studies show that the Fe2O3 NPs are covered by different biomolecular coronas in the intestine and liver and are accordingly internalized via distinct tissue-specific mechanisms. Our results are helpful for nanoparticle toxicological evaluation and environmental risk assessment in aquatic ecosystem.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"18 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Felipe H Dos Santos, Pavlo Ivanchenko, Roger Borges, Marcelo E Alves, Deb Jaisi, Marcos Kamogawa, Caue Ribeiro
{"title":"Bone-derived hydroxyapatite: Ultrastructure and tuning for controlled dissolution characteristics for a model nanofertilizer","authors":"Felipe H Dos Santos, Pavlo Ivanchenko, Roger Borges, Marcelo E Alves, Deb Jaisi, Marcos Kamogawa, Caue Ribeiro","doi":"10.1039/d5en00756a","DOIUrl":"https://doi.org/10.1039/d5en00756a","url":null,"abstract":"Calcium phosphate nanoparticles have been increasingly propounded as an efficient phosphorus (P) fertilizer in crop production. In this study, we used sustainable sourcing of P by recycling bovine bones and modifying the extracted hydroxyapatite using three approaches: calcination and mechano-activation, alkaline hydrolysis, and subcritical water extraction. Hydroxyapatites derived from these treatments were analyzed for ultrastructure, particle size distribution and crystal chemistry to interpret the dissolution characteristics under flow-through microfiltration system. While calcination was the most effective treatment for removing organic molecules—with only 0.22 wt.% of total organic carbon and 0.03 wt.% nitrogen remanining on hydroxyapatite—it led to the largest growth in crystallite size (113–139 nm) compared to hydroxyapatites treated through alkaline hydrolysis (45–93 nm) or subcritical water (33–78 nm). Surprisingly, the mechano-activated hydroxyapatite following the calcination exhibited a sustained and high P release profile, driven by the presence of ultrafine (10–35 nm) amorphous particles mixed with well-ordered and structurally defective structures. Hydroxyapatites recovered by alkaline hydrolysis and subcritical water treatments exhibited slow and steady P release profiles reflecting fewer structural and surface imperfections compared to mechano-activated hydroxyapatite. These findings demonstrate that processing induced ultrastructural and compositional changes in bone derived hydroxyapatite exert a major role in P‐release characteristics. Consequently, these products could be tuned for both sufficiency and efficiency of P fertilization, thus generating sustainably sourced nano P fertilizer for crop production.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"5 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jessica J Chadwick, Iuliia Mikulska, Swaroop Chakraborty, Aleksandar Radu, Peng Zhang, Sami Ullah, Iseult Lynch
{"title":"Soil-based zeolite and metal oxide nanomaterial application alters reactive Nitrogen losses and lettuce (Lactuca sativa L.) growth","authors":"Jessica J Chadwick, Iuliia Mikulska, Swaroop Chakraborty, Aleksandar Radu, Peng Zhang, Sami Ullah, Iseult Lynch","doi":"10.1039/d5en00526d","DOIUrl":"https://doi.org/10.1039/d5en00526d","url":null,"abstract":"Excessive nitrogen fertiliser use has resulted in reactive nitrogen losses to the environment through gaseous N emissions, like N<small><sub>2</sub></small>O, resulting in agriculture being a major anthropogenic source of N<small><sub>2</sub></small>O gas emissions globally. Using engineered nanomaterials to deliver reactive nitrogen can aid in more efficient nutrient delivery to crops, maximising yield and crop quality, while minimising reactive losses to the environment. ZSM-5-15, a nano-zeolite, increased cumulative N<small><sub>2</sub></small>O emissions by 134% when applied in combination with a 50% dose of conventional nitrogen fertiliser. This is theorised to be through ion exchange of ZSM-5-15’s extra-framework NH<small><sub>4</sub></small><small><sup>+</sup></small> ion load being released, allowing nitrifying microbes to act on the newly released NH<small><sub>4</sub></small><small><sup>+</sup></small> and increase N<small><sub>2</sub></small>O emissions. BEA-19, a similar zeolite to ZSM-5-15 but with a slightly altered Si:Al ratio, size and charge, causes no increase in N<small><sub>2</sub></small>O emissions. While ZSM-5-15 increases reactive N emissions it also drives improved lettuce growth, with 13% more biomass accumulation compared to a half dose of conventional fertiliser. Ce<small><sub>0.75</sub></small>Zr<small><sub>0.25</sub></small>O<small><sub>2</sub></small>, a nano-metal oxide, improves growth by 6% and maintains the nutritive quality of lettuce, with higher Zn, Cu, Mg, K, Fe and Mn contents, without increasing N<small><sub>2</sub></small>O emissions. Nano-Ce<small><sub>0.75</sub></small>Zr<small><sub>0.25</sub></small>O<small><sub>2</sub></small> transforms in soil to form CeO<small><sub>2</sub></small> and Ce<small><sub>0.9</sub></small>Zr<small><sub>0.1</sub></small>O<small><sub>2</sub></small>, leaching Zr<small><sup>4+</sup></small> ions some of which partly form ZrCl<small><sub>4</sub></small>. These compounds may then act on lettuce roots and soil microbes independently. These results indicate how nanomaterials may impact reactive nitrogen emissions through effects on soil microbial communities.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"19 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sonali Baksi, Km Madhuri Singh, Sangeeta Rani, Pallavi Sharma
{"title":"Melatonin-functionalized zinc oxide nanoparticles enhance salt stress tolerance in Vigna mungo L. by regulating antioxidants and ion homeostasis","authors":"Sonali Baksi, Km Madhuri Singh, Sangeeta Rani, Pallavi Sharma","doi":"10.1039/d5en00588d","DOIUrl":"https://doi.org/10.1039/d5en00588d","url":null,"abstract":"Salinity is a significant abiotic stress that significantly restricts plant development and production by inducing osmotic stress, disturbing ion homeostasis, and promoting oxidative injury. This research explored the impact of salt stress on Vigna mungo L. (black gram) and the ability of seed priming with zinc oxide NPs (ZnO NPs) and melatonin-functionalized ZnO NPs (MZ NPs) in conferring salinity stress mitigation. This is, to the best of our knowledge, the first work in examining MZ NPs' impact on plants under salinity stress. The salt stress significantly reduced growth indices, chlorophyll, carotenoids, and potassium contents and increased oxidative stress, osmoprotectants, sodium ion, and calcium ion levels. Among the tested treatments, MZ NPs provided the most significant enhancement in growth indices, followed by ZnO NPs and melatonin (MT) application in individual form. Seed priming with ZnO NPs and MZ NPs fortified plants against salt stress by stabilizing photosynthetic pigments, decreasing ion concentration, enhancing uptake of potassium, increasing activities of antioxidant enzymes, regulating ion transport, and suppressing sodium toxicity. Interestingly, MZ NPs possessed greater ability in restoring photosynthetic pigments, diminishing oxidative stress, optimizing activities of antioxidant enzymes, and downplaying sodium concentration. In addition, they completely recovered the contents of potassium and reduced excessive accumulation of osmoprotectants, revealing that they were coping well with stress in a balanced fashion. The findings demonstrate the complementary advantage in combining ZnO NPs with melatonin, presenting a promising strategy in enhancing salt stress tolerance in plants and encouraging the cultivation of crops in a sustainable way.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"93 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Environmentally persistent free radicals on micro- and nano-plastics: a double-edged sword to environmental and biological safety","authors":"Fangyu Hu, Sunyuxuan Lin, Kaili Wang, Mengxiang Cui, Jing Liu, Tao Wu, Chaoxiu Ren","doi":"10.1039/d5en00471c","DOIUrl":"https://doi.org/10.1039/d5en00471c","url":null,"abstract":"Environmentally persistent free radicals (EPFRs) are a new kind of pollutant that are stable and persistent in the environment and are mainly produced on particles or organics under heating or light irradiation. Aged micro- and nano-plastics (MNPs) are important sources of EPFRs. Existing reviews have focused particularly on the environmental and biological hazards of aged MNPs themselves. Few reviews have focused on the role of EPFRs on aged MNPs, let alone their fate in the environment and their positive applications. This review summarizes the factors that influence the occurrence and formation of EPFRs from aged MNPs, their types and decay periods in the environment, and their conversion into reactive species (RS). This article also discusses the environmental effects of EPFRs on microbial community structure, the cycles of elements (C, N, Cl, S, and P), and the survival of microorganisms, plants, and animals in soil and water. Moreover, the biological effects of EPFRs from aged MNPs are discussed, including oxidative stress, neurotoxicity, reproductive toxicity, respiratory toxicity, and their impact on aging. Meanwhile, the high reactivity of EPFRs can be harnessed to transform them into environmental protection warriors. Hence, the applications of EPFRs in improving environmental pollution are also reviewed, including their use in fixing metals, degrading organic pollutants (including antibiotics and MNPs), and sterilization. Finally, this text provides insights for future research on the dual nature of EPFRs on aged MNPs. This review aims to mitigate the environmental and biological risks of EPFRs and provide a reference for their optimal application.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"61 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145141187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Faisal, Aynur Bilmez, Mohammad Faizan, Yawar Habib, Haider Sultan, Abdulrahman A. Alatar, Ranjith Pathirana
{"title":"Recuperative potential of nano-biochar to alleviate arsenic toxicity in soybean (Glycine max): Modulation of uptake, morphological and biochemical properties","authors":"Mohammad Faisal, Aynur Bilmez, Mohammad Faizan, Yawar Habib, Haider Sultan, Abdulrahman A. Alatar, Ranjith Pathirana","doi":"10.1039/d5en00227c","DOIUrl":"https://doi.org/10.1039/d5en00227c","url":null,"abstract":"Arsenic (As) accumulation in soils is steadily rising, making it increasingly toxic to a variety of crop plants and humans. As reduce plant productivity by interfering with several molecular, biochemical, and morphological aspects of plant metabolism. Therefore, introducing new agents to address these issues is imperative. This study demonstrates the effective use of nano-biochar (nano-BC) to mitigate As stress toxicity in Glycine max (soybean) plants. We determined the effect of nano-BC (1% w/w) on mitigating As (50 µM) stress in soybean by examining various growth parameters and metabolic attributes. As stress inhibited plant height (by 51%) and net photosynthetic rate (by 50%) and caused the buildup of reactive oxygen species (ROS); however, nano-BC treatments significantly reversed all these parameters. Moreover, the As stress increased malondialdehyde (by 78%) and hydrogen peroxide (by 67%), which were partially reversed by nano-BC in the As-treated plants. This outcome may be attributed to activation of the plant defense response, particularly antioxidants, triggered by nano-BC. Overall, As tolerance in soybeans was positively regulated by nano-BC. However, additional research is required to fully understand the intricate mechanisms behind nano-BC and its defense mechanism against As.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"18 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Sandeep Raju, Rajarshi Panigrahi, Kirti Sankhala, Kumud Malika Tripathi
{"title":"Room Temperature Capture of Hazardous Gases by Sustainable N-doped Graphene Aerogel","authors":"K. Sandeep Raju, Rajarshi Panigrahi, Kirti Sankhala, Kumud Malika Tripathi","doi":"10.1039/d5en00660k","DOIUrl":"https://doi.org/10.1039/d5en00660k","url":null,"abstract":"The escalating release of anthropogenic volatile organic compounds (VOCs) and toxic gases (TGCs) have become a key environmental concern. The quest to address the complex capture challenges for VOCs/TGCs removal calls for innovative, advanced, highly efficient and sustainable materials. A straightforward, one-step, low-cost, sustainable and scalable technique was used for the synthesis of nitrogen-doped graphene aerogel (N-GA) from waste jaggery with 3D interconnected network, super-hydrophobicity, and high surface area. The efficiency of waste jaggery derived N-GA as multifunctional adsorbent for VOCs/TGCs under ambient conditions is investigated in both gaseous and liquid states in a reversible manner. The N-GA realizes the adsorption-based capture of diverse TGCs/VOCs such as dichloromethane (DCM), H2S, CS2, benzene and NH3 with adsorption capacity over 1226, 1002.07, 885.58 mg g-1, 792.9, 489.4 mg g-1, respectively with high regeneration capability over 10 cycles. The feasibility of N-GA for organics removal in aqueous medium has also been tested for diverse organic solvents. That is relevant for direct application in indoor/outdoor air purification technologies, water remediation and ecosystem protection.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"18 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zaiming Chen, Tianzhu Wang, Kaiyi Zhang, Jiajun Cheng, Meizhen Wang
{"title":"Goethite nanoparticles binding DNA in dissemination of antibiotic resistance genes: New insights into the role of inorganic phosphate under environmentally relevant concentrations","authors":"Zaiming Chen, Tianzhu Wang, Kaiyi Zhang, Jiajun Cheng, Meizhen Wang","doi":"10.1039/d5en00765h","DOIUrl":"https://doi.org/10.1039/d5en00765h","url":null,"abstract":"Iron (oxyhydr)oxides (FeO) significantly influence the environmental dissemination of antibiotic resistance genes (ARGs) by adsorbing ARG-carrying DNA through phosphate interactions. However, the fate of FeO-adsorbed DNA, particularly its release dynamics and impact on ARG dissemination in the presence of inorganic phosphate with environmentally relevant concentrations (Pie), remains unclear. Using goethite (a representative FeO mineral) and diverse DNA forms (three linear fragments, one ARG-carrying plasmid), this study quantified Pie-driven DNA desorption via a novel successive desorption-extraction protocol, distinguishing readily desorbable from residual DNA. Pie (1.0–10 mg-P/L) displaced 5%–96% of adsorbed DNA. Structurally, shorter linear DNA and supercoiled plasmid formed fewer Fe-O-P bonds per adsorbed molecule, enhancing Pie-driven displacement and subsequently increasing their desorbable fraction, yielding a two-stage response to Pie fluctuations (minimal below 0.2–0.5 mg P/L; substantial above). Critically, Escherichia coli transformation assays showed that while goethite adsorption suppressed ARG transfer, Pie-activated desorption restored transformation efficiency. These results resolve the unverified link between realistic Pie fluctuations (e.g., paddy field fertilization/sediment hydrology) and FeO-bound DNA release, demonstrating its potential role in ARG dissemination. This mechanistic insight is essential for risk assessment of ARG transmission in iron-rich ecosystems and strategic deployment of FeO materials for soil ARG mitigation.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"40 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}