生物学最新文献

EIciRNAs in focus: current understanding and future perspectives.

IF 3.6 3区生物学

RNA Biology Pub Date : 2025-12-01 Epub Date: 2024-12-23 DOI: 10.1080/15476286.2024.2443876

Yan Yang, Yinchun Zhong, Liang Chen

引用次数: 0

Identification of deleterious non-synonymous single nucleotide polymorphisms in the mRNA decay activator ZFP36L2.

IF 3.6 3区生物学

RNA Biology Pub Date : 2025-12-01 Epub Date: 2024-12-13 DOI: 10.1080/15476286.2024.2437590

Betül Akçeşme, Hilal Hekimoğlu, Venkat R Chirasani, Şeyma İş, Habibe Nur Atmaca, Justin M Waldern, Silvia B V Ramos

{"title":"Identification of deleterious non-synonymous single nucleotide polymorphisms in the mRNA decay activator ZFP36L2.","authors":"Betül Akçeşme, Hilal Hekimoğlu, Venkat R Chirasani, Şeyma İş, Habibe Nur Atmaca, Justin M Waldern, Silvia B V Ramos","doi":"10.1080/15476286.2024.2437590","DOIUrl":"https://doi.org/10.1080/15476286.2024.2437590","url":null,"abstract":"More than 4,000 single nucleotide polymorphisms (SNP) variants have been identified in the human ZFP36L2 gene, however only a few have been studied in the context of protein function. The tandem zinc finger domain of ZFP36L2, an RNA binding protein, is the functional domain that binds to its target mRNAs. This protein/RNA interaction triggers mRNA degradation, controlling gene expression. We identified 32 non-synonymous SNPs (nsSNPs) in the tandem zinc finger domain of ZFP36L2 that could have possible deleterious impacts in humans. Using different bioinformatic strategies, we prioritized five among these 32 nsSNPs, namely rs375096815, rs1183688047, rs1214015428, rs1215671792 and rs920398592 to be validated. When we experimentally tested the functionality of these protein variants using gel shift assays, all five (Y154H, R160W, R184C, G204D, and C206F) resulted in a dramatic reduction in RNA binding compared to the WT protein. To understand the mechanistic effect of these variants on the protein/RNA interaction, we employed DUET, DynaMut and PyMOL to investigate structural changes in the protein. Additionally, we conducted Molecular Docking and Molecular Dynamics Simulations to fine tune the active behaviour of this biomolecular system at an atomic level. Our results propose atomic explanations for the impact of each of these five genetic variants identified.","PeriodicalId":21351,"journal":{"name":"RNA Biology","volume":"22 1","pages":"1-15"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of adaptation to crowded larval environment on the evolution of sperm competitive ability in males of Drosophila melanogaster.

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2024-12-18 DOI: 10.1080/19336934.2024.2437204

Rohit Kapila, Komal Maggu, Neetika Ahlawat, Nagaraj Guru Prasad

{"title":"Effects of adaptation to crowded larval environment on the evolution of sperm competitive ability in males of Drosophila melanogaster.","authors":"Rohit Kapila, Komal Maggu, Neetika Ahlawat, Nagaraj Guru Prasad","doi":"10.1080/19336934.2024.2437204","DOIUrl":"https://doi.org/10.1080/19336934.2024.2437204","url":null,"abstract":"Two of the most important environmental factors that affect the sperm competitive ability in males are the availability of resources and the socio-sexual environment. Numerous studies have investigated the individual effects of these factors, but their combined effect on the evolution of sperm competitive ability remains untested. A crowded larval environment is unique because it simultaneously affects the fitness of the organism through both resource availability and the socio-sexual environment. In this study, we used a set of four laboratory populations of D. melanogaster, evolved under a crowded larval environment for more than 165 generations and their respective controls to investigate how the sperm competitive ability of the males is affected by a single generation of larval crowding versus evolution under a crowded larval environment for more than 165 generations. Our results show that larval crowding negatively affects the sperm defence ability of males evolved in a crowded larval environment, while it has no effect on the sperm defence ability of control males. Additionally, larval crowding negatively impacts the sperm offence ability in both control and evolved populations. Males from populations adapted to a crowded larval environment exhibit lower sperm offence ability at an older age compared to control populations.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2437204"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flavonoids from Polypodium hastatum as neuroprotective agents attenuate cerebral ischemia/reperfusion injury in vitro and in vivo via activating Nrf2.

IF 5.2 2区生物学

Redox Report Pub Date : 2025-12-01 Epub Date: 2024-12-19 DOI: 10.1080/13510002.2024.2440204

Huankai Yao, Ruiqing Wu, Dan Du, Fengwei Ai, Feng Yang, Yan Li, Suhua Qi

{"title":"Flavonoids from Polypodium hastatum as neuroprotective agents attenuate cerebral ischemia/reperfusion injury in vitro and in vivo via activating Nrf2.","authors":"Huankai Yao, Ruiqing Wu, Dan Du, Fengwei Ai, Feng Yang, Yan Li, Suhua Qi","doi":"10.1080/13510002.2024.2440204","DOIUrl":"https://doi.org/10.1080/13510002.2024.2440204","url":null,"abstract":"Objectives: Cerebral ischemic stroke is a leading cause of death worldwide. Though timely reperfusion reduces the infarction size, it exacerbates neuronal apoptosis due to oxidative stress. Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor regulating the expression of antioxidant enzymes. Activating Nrf2 gives a therapeutic approach to ischemic stroke.Methods: Herein we explored flavonoids identified from Polypodium hastatum as Nrf2 activators and their protective effects on PC12 cells injured by oxygen and glucose deprivation/restoration (OGD/R) as well as middle cerebral artery occlusion (MCAO) mice.Results: The results showed among these flavonoids, AAKR significantly improved the survival of PC12 cells induced by OGD/R and activated Nrf2 in a Keap1-dependent manner. Further investigations have disclosed AAKR attenuated oxidative stress, mitochondrial dysfunction and following apoptosis resulting from OGD/R. Meanwhile, activation of Nrf2 by AAKR was involved in the protective effects. Finally, it was found that AAKR could protect MCAO mice brains against ischemia/reperfusion injury via activating Nrf2.Discussion: This investigation could provide lead compounds for the discovery of novel Nrf2 activators targeting ischemia/reperfusion injury.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"30 1","pages":"2440204"},"PeriodicalIF":5.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of HGH1 in breast cancer cell lines can inhibit the viability, invasion and migration of tumor cells.

IF 3.3 3区生物学

Cell Adhesion & Migration Pub Date : 2025-12-01 Epub Date: 2024-12-18 DOI: 10.1080/19336918.2024.2442349

Zeyu Wang, Taiyuan Liu, Kang He, Longyun Wang, Xiaoxuan Ma, Zhaoyun Yang, Yingchao Zhang, Lijing Zhao

引用次数: 0

The role and function of lncRNA in ageing-associated liver diseases.

IF 3.6 3区生物学

RNA Biology Pub Date : 2025-12-01 Epub Date: 2024-12-19 DOI: 10.1080/15476286.2024.2440678

Peyman Kheirandish Zarandi, Mohsen Ghiasi, Mohammad Heiat

{"title":"The role and function of lncRNA in ageing-associated liver diseases.","authors":"Peyman Kheirandish Zarandi, Mohsen Ghiasi, Mohammad Heiat","doi":"10.1080/15476286.2024.2440678","DOIUrl":"https://doi.org/10.1080/15476286.2024.2440678","url":null,"abstract":"Liver diseases are a significant global health issue, characterized by elevated levels of disorder and death. The substantial impact of ageing on liver diseases and their prognosis is evident. Multiple processes are involved in the ageing process, which ultimately leads to functional deterioration of this organ. The process of liver ageing not only renders the liver more susceptible to diseases but also compromises the integrity of other organs due to the liver's critical function in metabolism regulation. A growing body of research suggests that long non-coding RNAs (lncRNAs) play a significant role in the majority of pathophysiological pathways. They regulate gene expression through a variety of interactions with microRNAs (miRNAs), messenger RNAs (mRNAs), DNA, or proteins. LncRNAs exert a major influence on the progression of age-related liver diseases through the regulation of cell proliferation, necrosis, apoptosis, senescence, and metabolic reprogramming. A concise overview of the current understanding of lncRNAs and their potential impact on the development of age-related liver diseases will be provided in this mini-review.","PeriodicalId":21351,"journal":{"name":"RNA Biology","volume":"22 1","pages":"1-8"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fast in situ hybridization chain reaction method in Drosophila embryos and ovaries.

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2024-12-05 DOI: 10.1080/19336934.2024.2428499

Kyohei Mikami, Yasuhiro Kozono, Masaki Masukawa, Satoru Kobayashi

引用次数: 0

Orosomucoid 1 interacts with S100A12 and activates ERK signalling to expedite the advancement of bladder cancer.

IF 3.3 3区生物学

Cell Adhesion & Migration Pub Date : 2025-12-01 Epub Date: 2024-12-07 DOI: 10.1080/19336918.2024.2434209

Zhe Liu, Xiaofeng Pu

引用次数: 0

A history of studies of reproductive isolation between Drosophila pseudoobscura and D. persimilis.

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2024-12-20 DOI: 10.1080/19336934.2024.2439111

Stewart Leigh, Michael G Ritchie

引用次数: 0

Activation of the mitochondrial unfolded protein response regulates the dynamic formation of stress granules. 线粒体未折叠蛋白反应的激活调节应激颗粒的动态形成。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-05-01 Epub Date: 2024-12-05 DOI: 10.1242/jcs.263548

Marta Lopez-Nieto, Zhaozhi Sun, Emily Relton, Rahme Safakli, Brian D Freibaum, J Paul Taylor, Alessia Ruggieri, Ioannis Smyrnias, Nicolas Locker

{"title":"Activation of the mitochondrial unfolded protein response regulates the dynamic formation of stress granules.","authors":"Marta Lopez-Nieto, Zhaozhi Sun, Emily Relton, Rahme Safakli, Brian D Freibaum, J Paul Taylor, Alessia Ruggieri, Ioannis Smyrnias, Nicolas Locker","doi":"10.1242/jcs.263548","DOIUrl":"10.1242/jcs.263548","url":null,"abstract":"To rapidly adapt to harmful changes to their environment, cells activate the integrated stress response (ISR). This results in an adaptive transcriptional and translational rewiring, and the formation of biomolecular condensates named stress granules (SGs), to resolve stress. In addition to this first line of defence, the mitochondrial unfolded protein response (UPRmt) activates a specific transcriptional programme to maintain mitochondrial homeostasis. We present evidence that the SG formation and UPRmt pathways are intertwined and communicate. UPRmt induction results in eIF2α phosphorylation and the initial and transient formation of SGs, which subsequently disassemble. The induction of GADD34 (also known as PPP1R15A) during late UPRmt protects cells from prolonged stress by impairing further assembly of SGs. Furthermore, mitochondrial functions and cellular survival are enhanced during UPRmt activation when SGs are absent, suggesting that UPRmt-induced SGs have an adverse effect on mitochondrial homeostasis. These findings point to a novel crosstalk between SGs and the UPRmt that might contribute to restoring mitochondrial functions under stressful conditions.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0