Acta biochimica et biophysica Sinica最新文献

Safari in the RNA world: a special issue focused on RNA biogenesis, functions, and technologies. RNA世界中的游猎：关注RNA生物发生、功能和技术的特刊。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-25 DOI: 10.3724/abbs.2024234

Ya-Nan Chang, Hong Cheng

引用次数: 0

Continuous carbon source supply is essential for high rifamycin productivity of Amycolatopsis mediterranei in nitrate-stimulated fermentation revealed by a metabolomic study.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-24 DOI: 10.3724/abbs.2024245

Qi Yan, Zhihui Shao, Chen Yang, Guoping Zhao

{"title":"Continuous carbon source supply is essential for high rifamycin productivity of Amycolatopsis mediterranei in nitrate-stimulated fermentation revealed by a metabolomic study.","authors":"Qi Yan, Zhihui Shao, Chen Yang, Guoping Zhao","doi":"10.3724/abbs.2024245","DOIUrl":"https://doi.org/10.3724/abbs.2024245","url":null,"abstract":"Amycolatopsis mediterranei U32 is an industrial strain capable of producing therapeutically useful rifamycin SV. In early days of fermentation studies, nitrate was found to increase the yield of rifamycin along with globally, affecting both carbon and nitrogen metabolism in favor of antibiotic biosynthesis; thus, the nitrate-stimulating effect (NSE) hypothesis was proposed. Although GlnR is likely the master regulator of the pleotropic effect of NSE, the global metabolism affected by NSE has never been systematically examined. In this study, we use mass spectrometry-based metabolomics to quantitatively monitor the metabolomic responses of A. mediterranei U32 to nitrate supplementation. The concentrations of many metabolites involved in central carbon metabolism, including glucose 6-phosphate, glucose 1-phosphate, UDP-glucose, and acetyl-coenzyme A, decrease significantly after the addition of 80 mM potassium nitrate to the medium. We find that the rifamycin SV production yield could be increased by the addition of glucose during the logarithmic growth phase. Moreover, at multiple time points during glucose supplementation in the mid- and late-exponential phases, the yield of rifamycin SV further increases, reaching 354.3%. Quantitative real-time PCR assays of the key genes corresponding to the synthesis of the rifamycin SV precursor combined with data from metabolomics analysis confirm that carbon source deficiency is compensated for after glucose supplementation and that the expression of genes involved in the pathway of 3-amino-5-hydroxybenzoic acid synthesis by UDP-glucose and glutamine is significantly increased. This preliminary exploration of dynamic metabolomic profiles has the potential to increase our understanding of the NSE.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melanoma-derived versican reactivates tumor-associated macrophages by upregulating pyruvate carboxylase through TLR2-MyD88-RelB axis under normoxia.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-24 DOI: 10.3724/abbs.2025011

Yuxin Shu, Linmin Zhou, Jinqin Qian, Wei-Guo Zhu

引用次数: 0

Head-to-head: IL-21 triumphs over IL-15 in NK cell therapy for glioblastoma.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-23 DOI: 10.3724/abbs.2025009

Jake C Miller, Bihui Cao, Jia Shen

引用次数: 0

Immunopathological characteristics and therapeutic effects of UC-MSCs in a pigeon breeder's lung mouse model.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-23 DOI: 10.3724/abbs.2025010

Jingran Xu, Li Li, Yaping Zhou, Zulipikaer Abudureheman, Lexin Xue, Chao Wu, Xiaoguang Zou

{"title":"Immunopathological characteristics and therapeutic effects of UC-MSCs in a pigeon breeder's lung mouse model.","authors":"Jingran Xu, Li Li, Yaping Zhou, Zulipikaer Abudureheman, Lexin Xue, Chao Wu, Xiaoguang Zou","doi":"10.3724/abbs.2025010","DOIUrl":"https://doi.org/10.3724/abbs.2025010","url":null,"abstract":"Hypersensitivity pneumonitis (HP), including pigeon breeder's lung (PBL), often progresses from acute inflammation to fibrosis, impairing lung function and limiting targeted therapeutic strategies. Mechanistic studies on PBL progression are limited by the lack of preclinical animal models and a predominant focus on patient data. This study explores the immunopathological characteristics of all stages of PBL in mice and evaluates the therapeutic potential of human umbilical cord-derived mesenchymal stem cells (UC-MSCs) during the non-fibrotic stage. PBL models are created in A/J mice through tracheal instillation of pigeon dropping extract (PDE) protein powder. Different doses (0.4 × 10 6, 0.8 × 10 6, and 1.6 × 10 6 cells per animal) and frequencies (1-2 times) are administered to the model. The immunopathological characteristics of PBL and the therapeutic effects of UC-MSCs are assessed using micro-CT, pulmonary function, histopathology, cell counts in BALF, HYP levels, inflammatory factor levels, immunohistochemistry, and fibrosis marker expression in lung tissues. The results show that PDE exposure consistently impairs pulmonary function and increases the levels of inflammation and fibrosis markers as the disease progresses. Model mice experience non-fibrotic stages (acute inflammation) from days 0-36, mild fibrosis from days 37-77, and severe fibrosis from day 78 onwards. UC-MSCs, particularly at the highest dose (1.6 × 10 6 cells), effectively treat non-fibrotic PBL by improving pulmonary function (lung ventilation area recovers) and reducing inflammation and fibrosis. This study successfully establishes PBL mouse models reflecting both the acute (inflammatory) and chronic (fibrotic) stages, and UC-MSCs have the potential to delay fibrosis, providing new therapeutic options for PBL and other inflammation-induced lung fibrotic diseases.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Daphnetin-mediated mitophagy alleviates intervertebral disc degeneration via the Nrf2/PINK1 pathway. 瑞香素介导的线粒体自噬通过Nrf2/PINK1途径缓解椎间盘退变。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-22 DOI: 10.3724/abbs.2025002

Yiting Tu, Jiaping Ren, Weiyuan Fang, Chencheng Zhou, Binli Zhao, Tianyong Hua, Yiqi Chen, Zhenya Chen, Yongzeng Feng, Haiming Jin, Xiangyang Wang

{"title":"Daphnetin-mediated mitophagy alleviates intervertebral disc degeneration via the Nrf2/PINK1 pathway.","authors":"Yiting Tu, Jiaping Ren, Weiyuan Fang, Chencheng Zhou, Binli Zhao, Tianyong Hua, Yiqi Chen, Zhenya Chen, Yongzeng Feng, Haiming Jin, Xiangyang Wang","doi":"10.3724/abbs.2025002","DOIUrl":"https://doi.org/10.3724/abbs.2025002","url":null,"abstract":"Intervertebral disc degeneration (IDD) is a major cause of low back pain (LBP), and effective therapies are still lacking. Reactive oxygen species (ROS) stress induces NLRP3 inflammasome activation, and this, along with extracellular matrix metabolism (ECM) degradation in nucleus pulposus cells (NPCs), plays a crucial role in the progression of IDD. Daphnetin (DAP) is a biologically active phytochemical extracted from plants of the Genus Daphne, which possesses various bioactivities, including antioxidant properties. In the present study, we demonstrate that DAP significantly attenuates tert-butyl hydroperoxide (TBHP)-induced ECM degradation, oxidative stress and NLRP3 inflammasome activation in NPCs. Furthermore, DAP could facilitate mitophagy to increase the removal of damaged mitochondria, consequently reducing mitochondrial ROS accumulation and alleviating NLRP3 inflammasome activation. Mechanistically, we unveil that DAP activates mitophagy by stimulating the Nrf2/PINK1 signaling pathway in TBHP-induced NPCs. In vivo experiments further corroborate the protective effect of DAP against IDD progression in a rat model induced by disc puncture. Accordingly, our findings reveal that DAP could be a promising therapeutic candidate for the treatment of IDD.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annexins: central regulators of plant growth and stress signaling. 膜联蛋白：植物生长和胁迫信号的中心调节因子。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-15 DOI: 10.3724/abbs.2024228

Chen Xie, Mingyue Zhu, Ruirui Shi, Liu Yang, Xiaoya An, Chao Wang

{"title":"Annexins: central regulators of plant growth and stress signaling.","authors":"Chen Xie, Mingyue Zhu, Ruirui Shi, Liu Yang, Xiaoya An, Chao Wang","doi":"10.3724/abbs.2024228","DOIUrl":"https://doi.org/10.3724/abbs.2024228","url":null,"abstract":"Annexins are a family of multifunctional calcium-dependent and phospholipid-binding proteins that are widely distributed in the plant kingdom. They have a highly conserved evolutionary history that dates back to single-celled protists. Plant annexins, as soluble proteins, can flexibly bind to endomembranes and plasma membranes, exhibiting unique calcium-dependent and calcium-independent characteristics. Members of the annexin family have diverse functions, including binding to F-actin, participating in ATP and GTP hydrolysis, and even serving as peroxidases or cation channels. Annexins play pivotal roles in plant growth and stress signaling. They can respond sensitively to environmental, metabolic, and developmental signals, thereby affecting cytoskeleton remodeling and exocytosis mechanisms. Plant annexin gene families have been successfully identified in multiple species, and their expression and intracellular localization are precisely regulated by developmental processes and environmental factors. Although research on plant annexins has aroused great interest, their depth and breadth still need further expansion compared with those of animal annexins. This article provides a comprehensive and in-depth review of the characteristics and functions of plant annexin families, revealing their core roles in plant growth and adaptation, and yielding valuable references and insights for future research.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATF3 triggers M2 macrophage polarization to protect against pulp inflammation through WNT4 regulation. ATF3通过WNT4调控触发M2巨噬细胞极化，保护牙髓炎症。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-13 DOI: 10.3724/abbs.2025005

Liu Liu, Jie Wang, Jie Yu, Jing Wang, Jinhua Yu

{"title":"ATF3 triggers M2 macrophage polarization to protect against pulp inflammation through WNT4 regulation.","authors":"Liu Liu, Jie Wang, Jie Yu, Jing Wang, Jinhua Yu","doi":"10.3724/abbs.2025005","DOIUrl":"https://doi.org/10.3724/abbs.2025005","url":null,"abstract":"Pulpitis is a common inflammatory oral disease that can lead to pulp necrosis. The aim of this study is to investigate the expression and regulatory mechanisms of ATF3, a potential therapeutic marker, in pulpitis. A mouse pulpitis model with different degrees of inflammation is established, and the expression of ATF3 in pulpitis is explored. The histological features of healthy pulp and pulpitis are analyzed by HE staining, and classical inflammatory factors are detected by immunohistochemistry (IHC). In an in vitro study, we investigate the role of ATF3 in the regulation of WNT4 transcription and explore the effects of the ATF3/WNT4 axis on the polarization of RAW264.7 macrophages, the inflammatory response and the osteogenic differentiation of human dental pulp stem/stromal cells (hDPSCs). Our results show that ATF3 is expressed at low levels in inflamed pulp tissues; overexpression of ATF3 reduces the area of pulp necrosis, decreases the level of pro-inflammatory factors, and promotes macrophage polarization toward the M2 type. Furthermore, we reveal that ATF3 binds to the WNT4 promoter region and positively regulates the expression of WNT4 and that ATF3 downregulates M1 markers and increases the expression of M2 markers by regulating WNT4 expression. In addition, ATF3 promotes the osteogenic differentiation of dental pulp stem cells. In summary, this study reveals that ATF3 promotes M2 macrophage polarization by regulating WNT4, which in turn inhibits pulpal inflammatory responses and promotes the osteogenic differentiation of dental pulp stem cells. These findings suggest that ATF3 may be a potential target for pulpitis treatment.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to: Excessive ER-phagy mediated by FAM134B contributes to trophoblast cell mitochondrial dysfunction in preeclampsia. 更正：FAM134B介导的过度er吞噬有助于子痫前期滋养细胞线粒体功能障碍。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-10 DOI: 10.3724/abbs.2025001

Andi Wang, Zhuo Li, Dan Zhang, Chang Chen, Hua Zhang

引用次数: 0

Validation of six commercially available angiotensin II type 1 receptor antibodies. 六种市售血管紧张素II型1受体抗体的验证。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-08 DOI: 10.3724/abbs.2024199

Bingjie Li, Xi Zhang, Fei Sun, Xingzhong Zhang, Huirong Liu, Suli Zhang

引用次数: 0