Journal of Agricultural and Food Chemistry最新文献

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N6-Methyladenosine Modification Regulates Prunus Necrotic Ringspot Virus Infection in Cucumis sativus n6 -甲基腺苷修饰调控黄瓜李坏死环斑病毒侵染
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-05-04 DOI: 10.1021/acs.jafc.5c00050
Zhenfei Dong, Na Liu, Zhixiang Zhang, Hongqing Wang, Shifang Li, Binhui Zhan
{"title":"N6-Methyladenosine Modification Regulates Prunus Necrotic Ringspot Virus Infection in Cucumis sativus","authors":"Zhenfei Dong, Na Liu, Zhixiang Zhang, Hongqing Wang, Shifang Li, Binhui Zhan","doi":"10.1021/acs.jafc.5c00050","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00050","url":null,"abstract":"N6-methyladenosine (m<sup>6</sup>A) is a common epigenetic modification found in eukaryotic RNA. Recent studies have increasingly highlighted the importance of m<sup>6</sup>A modification in plant defense against viruses. In this investigation, we found that prunus necrotic ringspot virus (PNRSV) infection affected the m<sup>6</sup>A modification process. Plant transcriptomes and epitranscriptomes were sequenced to coanalyze the dynamic changes of m<sup>6</sup>A modifications after PNRSV infection. Further studies revealed that the silencing of evolutionarily conserved C-terminal region (ECTs), encoding m<sup>6</sup>A readers, led to increased PNRSV accumulation, indicating that ECTs confer resistance to PNRSV. Additionally, we demonstrated that <i>UPF3</i> and <i>SMG7</i>, which are involved in non-sense-mediated mRNA decay pathways, as well as phenylalanine ammonia-lyase (PAL), a well-known key enzyme in plant defense and an identified m<sup>6</sup>A-modified gene following PNRSV infection, play crucial roles in regulating PNRSV infection. These findings provide new insights into understanding PNRSV infection and further elucidate the role of m<sup>6</sup>A in modulating viral infection in plants.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"36 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Healing from the Peel: Exploring the Bioactive Potential of Bananas for Gastric Ulcer Management 从果皮愈合:探索香蕉对胃溃疡管理的生物活性潜力
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-05-03 DOI: 10.1021/acs.jafc.4c12972
R. Lizeth P. Muñoz, Carolina P. Mora, Laura V. Parra-Perdomo, Giovanni Rojas
{"title":"Healing from the Peel: Exploring the Bioactive Potential of Bananas for Gastric Ulcer Management","authors":"R. Lizeth P. Muñoz, Carolina P. Mora, Laura V. Parra-Perdomo, Giovanni Rojas","doi":"10.1021/acs.jafc.4c12972","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c12972","url":null,"abstract":"Bananas (Musaceae), herbaceous plants widely cultivated in tropical and subtropical regions, are traditionally used for their purported therapeutic effects on early-stage gastric ulcers. This comprehensive review provides an analysis of the bioactive compounds in bananas, with a focus on the influence of varietal differences and ripening stages. Researchers have identified key bioactive molecules in bananas, including phenolic compounds, carotenoids, and biogenic amines, predominantly located in the banana peel. Unripe bananas contain higher levels of phenolic compounds and biogenic amines, whereas ripened bananas exhibit increased carotenoid content. Additionally, <i>in vivo</i> studies have indicated that flavonoids, particularly leucocyanidin, exert gastroprotective effects by enhancing gastric mucosal thickness and increasing epidermal growth factor receptor expression, which promotes angiogenesis and re-epithelialization of the gastric mucosa, thereby protecting against ulcer formation. The findings reinforce the medicinal value of bananas, particularly in their unripe state, and highlight the importance of further exploration into their bioactive components for the development of natural therapies targeting gastric health.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"280 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potassium Promotes Citric Acid Accumulation by Regulating Its Synthesis and Vacuole Storage in Newhall Navel Orange (Citrus sinensis) 钾通过调控纽荷尔脐橙的合成和液泡储存促进柠檬酸积累
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-05-02 DOI: 10.1021/acs.jafc.4c11984
Kongjie Wu, Chengxiao Hu, Yuqing Duan, Mohamed G. Moussa, Jinye Li, Wei Lou, Xuecheng Sun, Xiang Yan, Qiling Tan, Songwei Wu
{"title":"Potassium Promotes Citric Acid Accumulation by Regulating Its Synthesis and Vacuole Storage in Newhall Navel Orange (Citrus sinensis)","authors":"Kongjie Wu, Chengxiao Hu, Yuqing Duan, Mohamed G. Moussa, Jinye Li, Wei Lou, Xuecheng Sun, Xiang Yan, Qiling Tan, Songwei Wu","doi":"10.1021/acs.jafc.4c11984","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11984","url":null,"abstract":"Potassium (K) is recognized as a crucial element affecting fruit flavor quality and influences the accumulation of citric acid (CA). To gain deeper insights into how K regulates CA accumulation, we investigated CA synthesis in mitochondria, decomposition pathways, and vacuolar storage under both pot and field culture in Newhall navel orange (<i>Citrus sinensis</i>). Our findings demonstrated that an appropriate level of K enhanced CA concentrations primarily by modulating CA synthesis, as evidenced by the increased activities and gene expressions of citrate synthase (CS) and phosphoenolpyruvate carboxylase (PEPC) in the early stages of fruit development. Integration of mitochondrial proteomic and targeted metabolomic revealed that K promoted CA synthesis by accelerating the TCA cycle, which were supported by the upregulation of TCA cycle-associated proteins, including malate dehydrogenase, 2-oxoglutarate dehydrogenase E2 component, pyruvate dehydrogenase E2 component, and dihydrolipoamide dehydrogenase, along with elevated levels of TCA cycle-related metabolites such as CA, isocitrate, 2-oxoglutarate, and succinate. Additionally, K enhanced CA concentration in vacuoles by promoting vacuolar acidification, as evidenced by the upregulation of P-type ATPase (<i>CsPH8</i>) expression. In summary, our findings provide novel insights into K facilitating CA accumulation by modulating its synthesis and vacuole storage in Citrus.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"25 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization and Isolation of Ginger Phenols from Zingiber officinale Rhizomes through in Silico-Based Reverse Annotation and Molecular Networking 基于硅基反向注释和分子网络的生姜酚类化合物的鉴定与分离
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-05-02 DOI: 10.1021/acs.jafc.5c01166
Tang Zhou, Xiu-Qiong Zhang, Wen-Hao Qiao, Xiao-Dan Shao, Xing-Xi Li, Jun-Fang Dong, Li-Dong Shao, Wei-Yan Hu, Rong-Ping Zhang, Xing-Long Chen
{"title":"Characterization and Isolation of Ginger Phenols from Zingiber officinale Rhizomes through in Silico-Based Reverse Annotation and Molecular Networking","authors":"Tang Zhou, Xiu-Qiong Zhang, Wen-Hao Qiao, Xiao-Dan Shao, Xing-Xi Li, Jun-Fang Dong, Li-Dong Shao, Wei-Yan Hu, Rong-Ping Zhang, Xing-Long Chen","doi":"10.1021/acs.jafc.5c01166","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01166","url":null,"abstract":"Ginger is a rich source of ginger phenols with an anti-inflammatory property. Antineuroinflammatory pathways represent a promising therapeutic strategy for neurodegenerative diseases. Ginger was selected as our research object to discover bioactive constituents responsible for the antineuroinflammatory effect. An in silico-based reverse annotation strategy, in conjunction with molecular networking, was employed for the large-scale annotation of ginger phenols from ginger. A total of 174 ginger phenols and 235 ginger phenol dimers were annotated. Five new ginger phenols (<b>1–5</b>) and 18 known ginger phenols (<b>6–23</b>) were isolated following this proposed strategy, among which compound <b>1</b> was a new natural product and compounds <b>2–3</b> were novel ginger phenol dimers. Their structures were elucidated using extensive spectroscopic data and semisynthesis methods. Compounds <b>2</b>, <b>8</b>, <b>12</b>, <b>18</b>, and <b>19</b> exhibited a capacity to inhibit nitric oxide (NO) production and demonstrated antineuroinflammatory activity in lipopolysaccharide (LPS)-induced BV2 cells at the concentrations of 10, 20, and 40 μM. Enzyme-linked immunosorbent assay (ELISA) showed that compounds <b>2</b>, <b>18</b>, and <b>19</b> could significantly reduce the expression levels of cyclooxygenase-2 (COX-2) and interleukin-1β (IL-1β). In addition, compounds <b>2</b> and <b>18</b> displayed a certain inhibitory effect on the expressions of interleukin-10 (IL-10) and interleukin-6 (IL-6). The Western blot analysis revealed that compounds <b>18</b> and <b>19</b> effectively attenuated the expression of IL-1β, while only compound <b>2</b> inhibited the expression of phosphorylated nuclear factor kappa B (p-NF-κB) in BV2 cells. This study demonstrates the great potential and prospect of the proposed strategy in the utilization of ginger resources. Additionally, it reveals that ginger contains diverse ginger phenols with antineuroinflammation activity.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"111 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antifungal Activity and Potential Mechanisms of Two Bafilomycin Analogues Isolated from Streptomyces sp. NEAU-Y11 against Colletotrichum orbiculare Streptomyces sp. NEAU-Y11中两种巴霉素类似物对圆形炭疽菌的抑菌活性及其潜在机制
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-05-02 DOI: 10.1021/acs.jafc.5c03728
Wenshuai Song, Xiaoyan Yu, Xiaoxin Yu, Haifeng Zhang, Kuan Zhang, Lifeng Guo, Ji-Dong Wang, Dong-Lan Tian, Qingtao Yu, Xiangjing Wang, Junwei Zhao, Wensheng Xiang
{"title":"Antifungal Activity and Potential Mechanisms of Two Bafilomycin Analogues Isolated from Streptomyces sp. NEAU-Y11 against Colletotrichum orbiculare","authors":"Wenshuai Song, Xiaoyan Yu, Xiaoxin Yu, Haifeng Zhang, Kuan Zhang, Lifeng Guo, Ji-Dong Wang, Dong-Lan Tian, Qingtao Yu, Xiangjing Wang, Junwei Zhao, Wensheng Xiang","doi":"10.1021/acs.jafc.5c03728","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c03728","url":null,"abstract":"Cucumber anthracnose, caused by <i>Colletotrichum orbiculare</i>, severely affects the cucumber yield and quality. In this study, two active compounds, bafilomycin C<sub>1</sub> and JBIR-100, were isolated from strain NEAU-Y11 and exhibited strong antifungal activity against <i>C. orbiculare</i>, with EC<sub>50</sub> values of 0.0491 and 0.1042 μg/mL, respectively, significantly lower than those of the commercial fungicide (4.42 μg/mL). Pot experiments demonstrated effective control of cucumber anthracnose at 0.2 μg/mL for bafilomycin C<sub>1</sub> and 0.4 μg/mL for JBIR-100, with efficacies reaching 78.5 and 67.7%, respectively. Microscopy and biochemical analyses indicated that both compounds disrupted the fungal cell wall, membrane, and redox homeostasis, leading to cell death. Transcriptome analysis further revealed the effects of bafilomycin C<sub>1</sub>′s on amino acid metabolism, cell structure, redox homeostasis, and DNA double-strand break repair. These findings suggest that bafilomycin C<sub>1</sub> and JBIR-100 are promising candidates for use as agrochemical fungicides to control <i>C. orbiculares</i> and may serve as a basis for developing next-generation antifungal agents.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"76 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selenium: The Toxicant for Pathogen and Pest but the Guardian of Soil and Crop 硒:病原体和害虫的毒物,土壤和作物的守护者
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-05-02 DOI: 10.1021/acs.jafc.5c01293
Qin Cheng, Yin Wang, Chuang Han, Wenju Liu, Guocheng Fan, Huan Zhang, Zheng Lei, Chengxiao Hu, Xiaohu Zhao
{"title":"Selenium: The Toxicant for Pathogen and Pest but the Guardian of Soil and Crop","authors":"Qin Cheng, Yin Wang, Chuang Han, Wenju Liu, Guocheng Fan, Huan Zhang, Zheng Lei, Chengxiao Hu, Xiaohu Zhao","doi":"10.1021/acs.jafc.5c01293","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01293","url":null,"abstract":"Selenium (Se) is an essential micronutrient for higher organisms and plays a beneficial role in plant growth and development. In recent years, there has been growing interest in the using of Se to enhance plant resilience, particularly in mitigating the effects of diseases and pests in agricultural systems. This review offers a comprehensive analysis of the sources and chemical forms of Se in soil, investigates the mechanisms of plant uptake and metabolism of different Se forms, and evaluates the physical and chemical inhibition of pathogens by various Se forms, as well as the role of Se in enhancing plant systemic resistance for crop protection. Additionally, we summarize current research on the role of Se in pest and disease control and explore potential future research directions, with a focus on integrating Se into sustainable agricultural practices. The insights presented in this review seek to establish a solid scientific foundation for Se-based approaches to pest control and emphasize its potential application in sustainable agriculture.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"278 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Temporal Distribution of Imidacloprid and Its Metabolites in Laying Hens: An Evaluation of the Potential Impact on Food Safety 吡虫啉及其代谢物在蛋鸡体内的时间分布:对食品安全潜在影响的评价
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-05-02 DOI: 10.1021/acs.jafc.4c08378
Mayra F. Tsoi, Justin Zyskowski, Cara Robison, Andreas F. Lehner, Levent Dirikolu, John P. Buchweitz
{"title":"Temporal Distribution of Imidacloprid and Its Metabolites in Laying Hens: An Evaluation of the Potential Impact on Food Safety","authors":"Mayra F. Tsoi, Justin Zyskowski, Cara Robison, Andreas F. Lehner, Levent Dirikolu, John P. Buchweitz","doi":"10.1021/acs.jafc.4c08378","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08378","url":null,"abstract":"Imidacloprid (IMI) is widely used in poultry houses in the United States to control darkling beetles. However, there is limited knowledge regarding the persistence and distribution of IMI and its metabolites in poultry products following acute exposure to subclinical concentrations. In this study, mature hens received a dose of imidacloprid (placebo, 1 mg/kg, or 10 mg/kg), and liquid chromatography tandem mass spectrometry was used to quantify IMI and its metabolites in tissues. IMI was below the limit of quantitation in plasma within 24 (1 mg/kg) and 48 h (10 mg/kg). At 10 mg/kg, IMI equivalents (sum of IMI and its metabolites) exceeded the regulatory threshold as established by the United States Code of Federal Regulations for pectoral and thigh muscle, brain, liver, spleen, kidney, fat, and eggs after 24 h. At 1 mg/kg, IMI equivalents surpassed the threshold for liver, spleen, and eggs after 24 h. These results suggest that low dose exposure to IMI may cause poultry products destined for human consumption to violate federal regulations.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"15 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
1, 8-Cineole Ameliorated Staphylococcus aureus-Induced Pneumonia through Modulation of TRP-KYN and Arginine-NO Reprogramming 1,8 -桉树脑通过调节TRP-KYN和精氨酸- no重编程改善金黄色葡萄球菌诱导的肺炎
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-05-02 DOI: 10.1021/acs.jafc.4c10860
Qianwen Cao, Luyao Liu, Xiaoge Ma, Chaomin Zhong, Mengqi Tang, Mengge Liu, Ling-Bo Qu, Bo Wei, Xia Xu
{"title":"1, 8-Cineole Ameliorated Staphylococcus aureus-Induced Pneumonia through Modulation of TRP-KYN and Arginine-NO Reprogramming","authors":"Qianwen Cao, Luyao Liu, Xiaoge Ma, Chaomin Zhong, Mengqi Tang, Mengge Liu, Ling-Bo Qu, Bo Wei, Xia Xu","doi":"10.1021/acs.jafc.4c10860","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10860","url":null,"abstract":"1, 8-Cineole (Cin), a cyclic monoterpenoid derived from tea trees and eucalyptus species, exhibits diverse pharmacological properties. Yet, its therapeutic impact and underlying mechanism against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) pneumonia remain to be elucidated. In this study, metabolomics based on UPLC-MS/MS was integrated with network pharmacology, molecular biology, and molecular docking to investigate the effects of Cin. The findings demonstrated that Cin markedly reduced mortality and lung bacterial load, lessened pulmonary damage while suppressing the levels of proinflammatory factors, including tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the bronchoalveolar lavage fluid (BALF) of infected mice. Additionally, 19 metabolites, primarily involved in tryptophan metabolism and arginine biosynthesis, were notably modified by Cin via suppressing the enzymatic activity of indoleamine 2, 3-dioxygenase 1 (IDO1) and inducible nitric oxide synthase (iNOS), thereby attenuating the inflammatory response. Notably, knockdown of IDO1 or iNOS significantly diminished the anti-inflammation effect of Cin. In conclusion, our study validates the therapeutic potential of Cin against <i>S. aureus</i> pneumonia via anti-inflammation by downregulating IDO1 and iNOS. Our results provide a theoretical basis of natural substances applied in bacterial pneumonia treatment.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"43 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mechanistic Insights into Maltol-Mediated Reversal of Postmenopausal Osteoporosis via Regulation of CDK14 Ubiquitination in Macrophages 通过调节巨噬细胞中CDK14泛素化,麦芽糖醇介导的绝经后骨质疏松逆转的机制
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-05-02 DOI: 10.1021/acs.jafc.5c00545
Zhuoru Jin, Yufei Wei, Zimo Zhou, Zheng Fan, Ying Huang, Da Liu
{"title":"Mechanistic Insights into Maltol-Mediated Reversal of Postmenopausal Osteoporosis via Regulation of CDK14 Ubiquitination in Macrophages","authors":"Zhuoru Jin, Yufei Wei, Zimo Zhou, Zheng Fan, Ying Huang, Da Liu","doi":"10.1021/acs.jafc.5c00545","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00545","url":null,"abstract":"Maltol, primarily derived from Korean red ginseng, exhibits anti-inflammatory properties by modulating macrophage polarization and has potential therapeutic effects on postmenopausal osteoporosis, a condition linked to inflammation. This study explored the molecular mechanisms underlying maltol’s ability to inhibit M1 macrophage polarization and regulate osteoblast differentiation via macrophage-mediated pathways. Using in vitro and in vivo models, we demonstrated that maltol upregulates RNF213, which inhibits the CDK14-Pdgfrβ signaling pathway, suppressing M1 polarization and reducing NFκB phosphorylation and pro-inflammatory cytokine production. Additionally, maltol decreases TNFSF12 secretion, mitigating estrogen deficiency-induced osteoblast apoptosis and promoting differentiation. These findings highlight maltol’s potential in managing postmenopausal osteoporosis and other inflammatory diseases.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"53 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolic Engineering of Escherichia coli for Efficient l-Isoleucine Production based on the Citramalate Pathway 基于柠檬酸盐途径高效生产l-异亮氨酸的大肠杆菌代谢工程
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-05-02 DOI: 10.1021/acs.jafc.5c01949
Qiquan Zhang, Yuheng Wang, Xiaolu Wang, Yingguo Bai, Yaru Wang, Yuan Wang, Tao Tu, Xing Qin, Xiaoyun Su, Bin Yao, Huiying Luo, Xu Liu, Huoqing Huang, Jie Zhang
{"title":"Metabolic Engineering of Escherichia coli for Efficient l-Isoleucine Production based on the Citramalate Pathway","authors":"Qiquan Zhang, Yuheng Wang, Xiaolu Wang, Yingguo Bai, Yaru Wang, Yuan Wang, Tao Tu, Xing Qin, Xiaoyun Su, Bin Yao, Huiying Luo, Xu Liu, Huoqing Huang, Jie Zhang","doi":"10.1021/acs.jafc.5c01949","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01949","url":null,"abstract":"<span>l</span>-isoleucine, a value-added branched-chain amino acid, has been widely used in the food, feed, and pharmaceutical industries. However, the production efficiency of <span>l</span>-isoleucine is relatively low due to the complex and inefficient biosynthetic network of the canonical threonine pathway. Here, we report the exploitation of a concise citramalate pathway for the development of an efficient <span>l</span>-isoleucine producer. First, chassis strain and key genes were screened for establishment of the citramalate pathway. Subsequently, a citramalate importer was identified and applied to enhance citramalate’s utilization efficiency. Finally, a plasmid-free high-<span>l</span>-isoleucine producer was developed by enhancement of <span>l</span>-isoleucine efflux, optimization of rate-limiting gene expression, and introduction of a nonoxidative glycolysis pathway. Fed-batch fermentation of the final strain in a 10-L bioreactor produced 56.6 g/L <span>l</span>-isoleucine with a productivity of 1.66 g/L/h, which is the highest <span>l</span>-isoleucine titer and productivity reported. This study paves the way for construction of efficient microbial cell factories for production of <span>l</span>-isoleucine and related derivatives based on the citramalate pathway.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"11 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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