Journal of Agricultural and Food Chemistry最新文献

Efficient Heavy Metals Detection in Plants: A Microfluidic Approach Combined with Laser-Induced Breakdown Spectroscopy. 植物中重金属的高效检测：微流体方法与激光诱导击穿光谱相结合。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-03 DOI: 10.1021/acs.jafc.5c08130

Haotian Yang,Shijie Zhang,Chengxiang Duan,Yuanxin Wan,Yujie Shi,Xiaochan Wang,Gang Liu,Guo Zhao,Xiande Zhao

引用次数: 0

Targeted Proteomics Confirms Bovine Milk Peptide Signatures for Differentiating Healthy from Subclinical Mastitic Milk Infected with Streptococcus uberis and Staphylococcus aureus. 靶向蛋白质组学证实了区分感染uber链球菌和金黄色葡萄球菌的健康乳乳和亚临床乳乳的牛奶肽特征。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-03 DOI: 10.1021/acs.jafc.5c05984

Arpita Das,Kuldeep Giri,Sudipa Maity,Kiran Ambatipudi

{"title":"Targeted Proteomics Confirms Bovine Milk Peptide Signatures for Differentiating Healthy from Subclinical Mastitic Milk Infected with Streptococcus uberis and Staphylococcus aureus.","authors":"Arpita Das,Kuldeep Giri,Sudipa Maity,Kiran Ambatipudi","doi":"10.1021/acs.jafc.5c05984","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c05984","url":null,"abstract":"Bovine subclinical mastitis (SCM), an asymptomatic mammary inflammation, can lead to milk spoilage, premature culling of animals, and compromised milk quality. Our study aimed to identify and establish the functionality of milk proteins as potential markers for SCM state utilizing multiple reaction monitoring mass spectrometry (MRM-MS). In our study, 464 protein groups were confidently identified, of which 215 protein groups showed relatively different expression between healthy (H) and SCM. The MRM assay was used to quantify peptides NLQI and SLFS (lactophorin), EITQ (serum-amyloid-A), WEEA (leukotriene-A4-hydrolase), and LISD (kininogen) on a cohort of 30 animals in each animal group for evaluating their diagnostic potential and discriminating performances. Compared to one protein's effectiveness as an individualistic marker, the synergistic pattern of multiple peptides achieved better discriminating power toward SCM from healthy individuals with sensitivity (>0.90) and specificity (>0.90). The study's results established peptide signatures in milk, distinguishing the SCM from the H group.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"75 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Characterization of a Fatty Acyl Reductase Identified from the Female Pheromone Gland of Mythimna loreyi (Lepidoptera: Noctuidae). 一种从金翅目：夜蛾科雌费洛蒙腺中鉴定的脂肪酸酰还原酶的功能特征。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c08131

Liuyang Wang,Pei Guo,Xiaofang Zhang,Jianqing Guo,Runjie Meng,Jun Ning,Tao Zhang

{"title":"Functional Characterization of a Fatty Acyl Reductase Identified from the Female Pheromone Gland of Mythimna loreyi (Lepidoptera: Noctuidae).","authors":"Liuyang Wang,Pei Guo,Xiaofang Zhang,Jianqing Guo,Runjie Meng,Jun Ning,Tao Zhang","doi":"10.1021/acs.jafc.5c08131","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c08131","url":null,"abstract":"Sex pheromones, which play a critical role in mediating sexual communication of Lepidoptera, are biosynthesized by a variety of particular enzymes in specialized pheromone glands (PGs). Among these enzymes, fatty acyl-CoA reductases (FARs) catalyze the reduction of fatty acyl precursors to their corresponding alcohols. In this study, we identified 32 FARs from the genome of Mythimna loreyi, an important agricultural pest. Among all MlorFARs identified, only MlorFAR1 showed a PG expression pattern and exhibited a positive response to pheromone biosynthesis activating neuropeptide (PBAN) injection. Silencing MlorFAR1 reduced pheromone titers by 70.4-88.9% and significantly reduced mating rates at the 48-72 h postinjection time point. Heterologous expression in yeast confirmed that MlorFAR1 preferentially converted medium-chain fatty acid methyl esters to alcohols. These findings establish MlorFAR1 as a pivotal enzyme in the biosynthesis of sex pheromones in M. loreyi, providing a framework for the development of innovative pest management strategies.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"28 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Type B Trichothecenes in Grain-Based Foods: Method Validation and Analysis of Convenience Survey Samples. 谷物类食品中的B型毛霉烯：方便调查样品的方法验证与分析。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c09422

Kai Zhang,Brenna M Flannery,Selena Jiang

引用次数: 0

Resistance Evolution and Fitness of Fusarium asiaticum from Fields to Succinate Dehydrogenase Inhibitor Pydiflumetofen in China. 中国田间亚洲镰刀菌对琥珀酸脱氢酶抑制剂吡氟醚的抗性进化及适应性研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c07082

Zhili Yuan,Huina Ma,Niu Ma,Qi Wu,Zelong Peng,Chunjuan Yan,Yiping Hou

{"title":"Resistance Evolution and Fitness of Fusarium asiaticum from Fields to Succinate Dehydrogenase Inhibitor Pydiflumetofen in China.","authors":"Zhili Yuan,Huina Ma,Niu Ma,Qi Wu,Zelong Peng,Chunjuan Yan,Yiping Hou","doi":"10.1021/acs.jafc.5c07082","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c07082","url":null,"abstract":"Pydiflumetofen, a succinate dehydrogenase inhibitor (SDHI), has been widely used to control Fusarium head blight (FHB) caused by Fusarium asiaticum since 2020. Resistance monitoring across different provinces in China from 2020 to 2023 detected no pydiflumetofen-tolerant or resistant F. asiaticum isolates. In 2024, 58 out of 539 F. asiaticum isolates (10.8%) collected from fields in China were identified as pydiflumetofen-tolerant (Pyd-TR), with their sensitivity distribution displaying a continuous rightward shift as EC50 values increased from 0.0168 to 0.0225 μg/mL and MIC values increased from 0.500 to 5.00 μg/mL. Pyd-TR exhibited growth, reproduction, and pathogenicity comparable to those of sensitive isolates. Cross-resistance was observed between pydiflumetofen and fluopyram but not with non-SDHI. No mutations were detected in SdhB/C1/C2/D of Pyd-TR. However, the expression of SdhC1 in Pyd-TR was significantly higher than in sensitive isolates after pydiflumetofen treatment. This study provides the first evidence of the early resistance evolution of F. asiaticum to pydiflumetofen in fields, which is associated with SdhC1 overexpression.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"94 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of Intestinal Barrier Function by Butyryl-Fructooligosaccharides via Microbiota-Host Interactions in Colonic Organoid Models. 在结肠类器官模型中，低聚丁基果糖通过微生物-宿主相互作用增强肠道屏障功能。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c09432

Hanluo Li,Linda Liu,Jiaxin Liu,Hang Zheng,Jing Long,Jingyi Wang,Seockmo Ku,Meilin Weng,Sini Kang

{"title":"Enhancement of Intestinal Barrier Function by Butyryl-Fructooligosaccharides via Microbiota-Host Interactions in Colonic Organoid Models.","authors":"Hanluo Li,Linda Liu,Jiaxin Liu,Hang Zheng,Jing Long,Jingyi Wang,Seockmo Ku,Meilin Weng,Sini Kang","doi":"10.1021/acs.jafc.5c09432","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c09432","url":null,"abstract":"Butyryl-fructooligosaccharides (B-FOSs) are prebiotic derivatives synthesized by ester-linked conjugation of fructooligosaccharides (FOSs) with butyrate, exhibiting enhanced prebiotic capabilities over conventional FOSs. However, their therapeutic mechanisms remain incompletely characterized. Our in vitro analyses revealed that B-FOSs resist gastrointestinal digestion and undergo fecal microbial fermentation, indicating their capacity to deliver butyrate to the colon. By integrating in vitro fecal fermentation with 3D colonoids derived from Lgr5+ intestinal stem cells, we systematically explored B-FOS-mediated microbiota-metabolite interactions. Organoid proliferation and viability were enhanced by B-FOS metabolites and a reshaped gut microbiota, which also counteracted liposaccharide (LPS)-induced epithelial disruption by upregulating ZO-1 and MUC2 expression. Notably, B-FOS-fermented supernatants demonstrated superior barrier-protective efficacy than was achieved using equivalent doses of butyrate, suggesting effects of other microbiota-derived metabolites. The B-FOS-modulated microbiota outperformed bacterial controls in terms of MUC2 and ZO-1 production, with Weissella identified as a critical degrader driving B-FOS metabolism and mucin biosynthesis.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"17 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porous Microspheres Promote Rice Growth and Restore the Health of Contaminated Soil by Activating Beneficial Soil Microorganisms. 多孔微球通过激活有益土壤微生物促进水稻生长和恢复污染土壤的健康。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c07079

Qizhen Zhang,Xin Shi,Rongjia Zhou,Wanzhen Deng,Tuqiang Gao,Mingming Yin,Pengfei Liu

{"title":"Porous Microspheres Promote Rice Growth and Restore the Health of Contaminated Soil by Activating Beneficial Soil Microorganisms.","authors":"Qizhen Zhang,Xin Shi,Rongjia Zhou,Wanzhen Deng,Tuqiang Gao,Mingming Yin,Pengfei Liu","doi":"10.1021/acs.jafc.5c07079","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c07079","url":null,"abstract":"Heavy metals and pesticides are persistent and toxic contaminants that are frequently present in agricultural soils. In this study, we developed porous microspheres (MS) using sodium alginate and carboxymethyl cellulose as composite carriers. Compared with untreated contaminated soils (copper, Cu; hymexazol, HML), MS treatment (MS-Cu, MS-HML) significantly improved soil pH (by up to 2.21%), electrical conductivity (reduction up to 35.12%), and increased nitrogen and phosphorus contents (nitrate nitrogen by 7.19-41.79%, available phosphorus by 4.42-24.58%). Urease and sucrase activities were enhanced by 6.74-27.10% from weeks 2 to 7. Moreover, the relative abundance of soil fungal communities increased with enrichment of beneficial fungi associated with heavy metal sequestration and plant growth promotion. MS addition reduced the accumulation of copper and HML in rice roots by 18.46% and 18.67%, respectively, thereby alleviating adverse effects on seed germination and seedling growth. These findings offer a promising strategy for the sustainable remediation of contaminated soils.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"78 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Monitoring of Isomalto/Malto-Polysaccharide Formation by Different 4,6-α-Glucanotransferases. 不同4,6-α-葡聚糖转移酶对异麦芽/麦芽糖形成的影响

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c07954

Nele Brand,Oliver Müller,Daniel Wefers

引用次数: 0

Methoxyfenozide and Lufenuron Enhanced Insecticidal Activity via 20E Biosynthesis-Immunity Dual Disruption in Spodoptera exigua (Lepidoptera, Noctuidae). 甲氧虫酰肼和氟虫腈通过20E生物合成-免疫双重干扰增强对夜蛾夜蛾的杀虫活性。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c10854

Zhixian Zhang,Dan Wang,Yajie Ma,Yongpan Shan,Xianpeng Song,Hongyan Hu,Changcai Wu,Yan Ma,Xiangliang Ren

{"title":"Methoxyfenozide and Lufenuron Enhanced Insecticidal Activity via 20E Biosynthesis-Immunity Dual Disruption in Spodoptera exigua (Lepidoptera, Noctuidae).","authors":"Zhixian Zhang,Dan Wang,Yajie Ma,Yongpan Shan,Xianpeng Song,Hongyan Hu,Changcai Wu,Yan Ma,Xiangliang Ren","doi":"10.1021/acs.jafc.5c10854","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c10854","url":null,"abstract":"Spodoptera exigua (Lepidoptera, Noctuidae) poses significant threats to global agriculture, necessitating the management of resistance through sustainable alternatives such as Insect growth regulators (IGRs), including Methoxyfenozide (MET) and Lufenuron (LUF). This study elucidates the potential regulatory mechanisms between 20-hydroxyecdysone (20E) and immune signaling under IGR exposure. Sublethal doses of MET, LUF, and their mixture (MML) significantly downregulated the 20E titer by upregulating SeShd and SeCYP18A1. Silencing SeShd decreased 20E, whereas knockdown SeCYP18A1 increased 20E; both delayed development and increased susceptibility to IGRs. Furthermore, IGRs activated immune pathways (Toll/IMD), elevating SeDif and SePGRP-LB (SePLB) expression while simultaneously suppressing 20E. Silencing SeDif and SePLB stimulated 20E biosynthesis and heightened susceptibility to IGRs. These findings offer novel insights into the intricate interplay between 20E biosynthesis and the immune response to IGRs. Our work enhances the understanding of the IGRs' molecular mechanism and offers actionable strategies for combating resistance in S. exigua.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"3 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Tartaric Acid/Sodium Tartrate Buffer System for Xylo-Oligosaccharide Production from Moso Bamboo. 一种新型酒石酸/酒石酸钠缓冲体系用于毛竹生产低聚木糖。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c08783

Juan Wang,Wenjun Ying,Peiyao Wen,Hong Liao,Junhua Zhang

引用次数: 0