Journal of Agricultural and Food Chemistry最新文献

Recent Advances in Developing Optical and Electrochemical Sensors for Monitoring Thiram and Future Perspectives 开发用于监测 Thiram 的光学和电化学传感器的最新进展和未来展望

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-13 DOI: 10.1021/acs.jafc.4c06107

Chenfei Wang, Zihan Zhu, Xinda Huang, Xuan Wang, Li Zhang, Yue Peng, Rongyan Wan, Lirong Han, Linsen Li, Xinhong Qin, Haiyin Li, Jianling Chen

{"title":"Recent Advances in Developing Optical and Electrochemical Sensors for Monitoring Thiram and Future Perspectives","authors":"Chenfei Wang, Zihan Zhu, Xinda Huang, Xuan Wang, Li Zhang, Yue Peng, Rongyan Wan, Lirong Han, Linsen Li, Xinhong Qin, Haiyin Li, Jianling Chen","doi":"10.1021/acs.jafc.4c06107","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06107","url":null,"abstract":"Thiram, as one widely used dithiocarbamate pesticide, has been considered seriously detrimental to food safety and human health because of poor efficiency, nonstandard/superfluous usage, and lack of a targeting effect. Developing high-performance sensors for thirams is strongly needed. With the rapid development of chemistry, biology, and materials science, many sensors have been constructed for thiram with high sensitivity and selectivity. Regarding the energy form of the signal, recognition mode, and detection principle, recent advances in the design and construction of optical and electrochemical sensors for thiram are summarized in this review, including colorimetric, luminescent, chemiluminescent, and electrochemical sensors. The advantages and disadvantages of the sensors for thiram including sensitivity, ability to avoid interference, recognition mechanism, signal output mode, and practicability are clarified in detail. Furthermore, the challenges faced, effective restrictions, and next direction of development are proposed for achieving more sensitive and selective analysis of thiram with less interference. We desire that this review will supply a solid theoretical basis and inspiration to generate innovative thinking for achieving new progress on thiram assays and the commercialization of the developed sensors in the future.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142430430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “The Fungal Transcription Factor BcTbs1 from Botrytis cinerea Promotes Pathogenicity via Host Cellulose Degradation” 对 "灰葡萄孢真菌转录因子 BcTbs1 通过宿主纤维素降解促进致病性 "的更正

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-12 DOI: 10.1021/acs.jafc.4c09198

Yinshan Zhang, Yue Liu, Chengguo Jia, Jianchun Qin, Bin Li, Wuliang Shi, Yubin Zhang, Jie Hou, Qingming Qin, Mingzhe Zhang, Guihua Li

引用次数: 0

Design, Synthesis, and Biological Activity Evaluation of Eco-Friendly Rosin-Diamide-Based Fungicides as Potential Succinate Dehydrogenase Inhibitors for Sustainable Crop Protection 设计、合成和生物活性评估环保型松香-二酰胺基杀菌剂，将其作为潜在的琥珀酸脱氢酶抑制剂用于可持续作物保护

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-11 DOI: 10.1021/acs.jafc.4c04634

Renle Xu, Yuhang Lou, Jinhang Ma, Xu Han, Yanqing Gao, Shibin Shang, Zhanqian Song, Jian Li

{"title":"Design, Synthesis, and Biological Activity Evaluation of Eco-Friendly Rosin-Diamide-Based Fungicides as Potential Succinate Dehydrogenase Inhibitors for Sustainable Crop Protection","authors":"Renle Xu, Yuhang Lou, Jinhang Ma, Xu Han, Yanqing Gao, Shibin Shang, Zhanqian Song, Jian Li","doi":"10.1021/acs.jafc.4c04634","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c04634","url":null,"abstract":"To develop novel succinate dehydrogenase (SDH) inhibitors for sustainable crop protection, a series of dehydroabietyl-diamide-based fungicides (a total of 21) were designed. In vitro fungicidal activity measurement showed that compound 3u exhibited excellent fungicidal activity against Valsa mali (half-maximal effective concentration, EC50 = 0.195 μg/mL), surpassing that of the positive control carbendazim (EC50 = 1.35 μg/mL). The in vivo fungicidal activity assessment suggested that 3u exhibited a protective effect on apple branches (69.7–48.1%) and apples (94.6–56.6%). Furthermore, biosafety evaluation indicated that 3u was significantly environmentally friendly toward zebrafish. Subsequently, morphology, physiology, and molecular docking were investigated to elucidate the mode of action of 3u against V. mali. Results demonstrated a strong binding between 3u and SDH, resulting in decreased SDH activity (half-maximal inhibitory concentration, IC50 = 11.7 μg/mL). Moreover, 3u disrupted the mycelial cell membrane and accelerated electrolyte leakage, ultimately resulting in the death of V. mali. These findings suggest that 3u could serve as a potent SDH inhibitor for sustainable crop protection.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitinase and Deacetylase-Based Chitin-Degrading Bacteria: One-Pot Cascade Bioconversion of Chitin to Chitooligosaccharides. 基于几丁质酶和脱乙酰酶的几丁质降解细菌：甲壳素到壳寡糖的单锅级联生物转化。

IF 5.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-11 DOI: 10.1021/acs.jafc.4c07053

Liwei Qing, Jing Gao, Liuhuan Du, Yiying Liu, Na Guo, Jianan Sun, Hao Dong, Xiangzhao Mao

{"title":"Chitinase and Deacetylase-Based Chitin-Degrading Bacteria: One-Pot Cascade Bioconversion of Chitin to Chitooligosaccharides.","authors":"Liwei Qing, Jing Gao, Liuhuan Du, Yiying Liu, Na Guo, Jianan Sun, Hao Dong, Xiangzhao Mao","doi":"10.1021/acs.jafc.4c07053","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07053","url":null,"abstract":"Cascade conversion of chitin into soluble and functional chitooligosaccharides has gained great attention. However, the biotransformation route is still limited to the low catalytic performances of chitin deacetylases (CDAs) and complicated procedures. In this study, a CDA from Arthrobacter sp. Jub115 (ArCDA) was identified and characterized, which showed a higher catalytic stability than the reported CDAs, with residual activity of 80.49%, 71.12%, and 56.09% after incubation at 30, 35, and 40 °C for 24 h, respectively. Additionally, ArCDA was identified to have a broad substrate spectrum toward β-chitin and N-acetyl chitooligosaccharides. Moreover, an engineered chitin-degrading bacteria (CDB) with cell-surface-displayed deacetylase ArCDA and chitinase SaChiB was constructed to simplify catalysis procedures, facilitating the chitobiose production of 294.30 ± 16.43 mg/L in 10 h. This study not only identified a CDA with the desirable catalytic performance but also provided a strategy for constructing CDB, facilitating the high-value utilization of chitin.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary Dihydroquercetin Alleviated Colitis via the Short-Chain Fatty Acids/miR-10a-5p/PI3K-Akt Signaling Pathway 膳食二氢槲皮素通过短链脂肪酸/miR-10a-5p/PI3K-Akt 信号通路缓解结肠炎

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-11 DOI: 10.1021/acs.jafc.4c03278

Tong Liu, Siqing Fan, Pengfei Meng, Mingxia Ma, Yanxin Wang, Jiaojiao Han, Yufei Wu, Xiao Li, Xiurong Su, Chenyang Lu

{"title":"Dietary Dihydroquercetin Alleviated Colitis via the Short-Chain Fatty Acids/miR-10a-5p/PI3K-Akt Signaling Pathway","authors":"Tong Liu, Siqing Fan, Pengfei Meng, Mingxia Ma, Yanxin Wang, Jiaojiao Han, Yufei Wu, Xiao Li, Xiurong Su, Chenyang Lu","doi":"10.1021/acs.jafc.4c03278","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c03278","url":null,"abstract":"Gut microbiota provides an important insight into clarifying the mechanism of active substances with low bioavailability, but its specific action mechanism varied case by case and remained unclear. Dihydroquercetin (DHQ) is a bioactive flavonoid with low bioavailability, which showed beneficial effects on colitis alleviation and gut microbiota modulation. Herein, we aimed to explore the microbiota-dependent anticolitis mechanism of DHQ in sight of gut microbiota metabolites and their interactions with microRNAs (miRNAs). Dietary supplementation of DHQ alleviated dextran sulfate sodium-induced colitis phenotypes and improved gut microbiota dysbiosis. Fecal microbiota transplantation further revealed that the anticolitis activity of DHQ was mediated by gut microbiota. To clarify how the modulated gut microbiota alleviated colitis in mice, the tandem analyses of the microbiome and targeted metabolome were performed, and altered profiles of metabolite short-chain fatty acids (SCFAs) and bile acids and their producers were observed in DHQ-treated mice. In addition, SCFA treatment showed anticolitis activity compared to that of bile acids, along with the specific inhibition on the phosphoinositide-3-kinase (PI3K)-protein kinase B (Akt) pathway. Subsequently, the colonic miRNA profile of mice receiving SCFA treatment was sequenced, and a differentially expressed miR-10a-5p was identified. Both prediction analysis and dual-luciferase reporter assay indicated that miR-10a-5p directly bind to the 3′-untranslated regions of gene pik3ca, inhibit the PI3K-Akt pathway activation, and lead to colitis alleviation. Together, we proposed that gut microbiota mediated the anticolitis activity of DHQ through the SCFAs/miR-10a-5p/PI3K-Akt axis, and it provided a novel insight into clarifying the microbiota-dependent mechanism via the interaction between metabolites and miRNAs.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Translating 2D-Chromatographic Fingerprinting to Quantitative Volatilomics: Unrevealing Compositional Changes in Maize Silage Volatilome for Robust Marker Discovery 将二维色谱指纹图谱转化为定量挥发物组学：揭示玉米青贮挥发物的成分变化，发现可靠的标记物

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-11 DOI: 10.1021/acs.jafc.4c05877

Andrea Caratti, Francesco Ferrero, Ernesto Tabacco, Fabio Gerbaldo, Erica Liberto, Giorgio Borreani, Chiara Cordero

{"title":"Translating 2D-Chromatographic Fingerprinting to Quantitative Volatilomics: Unrevealing Compositional Changes in Maize Silage Volatilome for Robust Marker Discovery","authors":"Andrea Caratti, Francesco Ferrero, Ernesto Tabacco, Fabio Gerbaldo, Erica Liberto, Giorgio Borreani, Chiara Cordero","doi":"10.1021/acs.jafc.4c05877","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c05877","url":null,"abstract":"This study examines the complex volatilome of maize silage, both with and without commercial heterolactic strain inoculation, conserved for 100 days, using quantitative volatilomics. Chemical classes linked to microbial metabolism were analyzed across a concentration range from 10 μg g–1 to 1 ng g–1. A reference method using comprehensive two-dimensional gas chromatography (GC × GC) and time-of-flight mass spectrometry (TOF MS) with loop-type thermal modulation (TM) was translated to a differential-flow modulation (FM) platform with parallel MS and flame ionization detector (FID) detection. With translation, the original method’s analyte elution order and resolution are preserved. The new method allowed for accurate quantification using multiple headspace solid-phase microextraction (MHS-SPME) and FID-predicted relative response factors (RRFs). Both methods showed comparable discriminatory power with FM GC × GC-MS/FID achieving satisfactory quantification accuracy without external calibration. Analysis of 98 volatiles provided insights into silage fermentation, supporting marker discovery and correlations with silage quality and stability.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precise Immobilization Strategy Combined with Rational Design to Improve β-Agarase Stability 精确固定化策略与合理设计相结合，提高 β-Agarase 的稳定性

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-11 DOI: 10.1021/acs.jafc.4c06466

Xuewu Liu, Xingfei Li, Qiaoling Xie, Cheng Lu, Zhengjun Xie, Xing Zhou, Long Chen, Chao Qiu, Zhengyu Jin, Jie Long

{"title":"Precise Immobilization Strategy Combined with Rational Design to Improve β-Agarase Stability","authors":"Xuewu Liu, Xingfei Li, Qiaoling Xie, Cheng Lu, Zhengjun Xie, Xing Zhou, Long Chen, Chao Qiu, Zhengyu Jin, Jie Long","doi":"10.1021/acs.jafc.4c06466","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06466","url":null,"abstract":"Recently, the orientational immobilization of enzymes has attracted extensive attention. In this study, we report the development of a strategy combined with rational design to achieve precise site-specific covalent immobilization of β-agarase. We first rationally screened six surface sites that can be mutated to cysteine by combining molecular dynamics simulation and energy calculation. Site-specific immobilization was successfully achieved by Michael addition reaction of mutant enzymes and maleimide-modified magnetic nanoparticles (MAL-MNPs). The enzyme activity retention rate of R66C-MAL-MNPs and K588C-MAL-MNPs was greater than 96%. The thermal deactivation kinetics study revealed that the site-specific immobilization strategy significantly improved the thermal stability of Aga50D, resulting in a substantial increase in its antidenaturation activity at elevated temperatures, and the highest t1/2 of the immobilized mutant enzymes was increased by an impressive 21.25-fold at 40 °C. The immobilized mutant enzymes also showed significantly enhanced tolerance to metal ions and organic reagents. For instance, all of the immobilized enzymes maintained over 90% of their enzymatic activity in the 50% (v/v) acetone/water solution. The present work may pave the way for the design of precisely immobilized enzymes, which can help promote green manufacturing.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Complexation with Different Molecular Weights of Oat β-Glucan and Sea Buckthorn Flavonoid on the Digestion of Rice Flour 不同分子量的燕麦β-葡聚糖和沙棘黄酮络合物对米粉消化的影响

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-11 DOI: 10.1021/acs.jafc.4c06405

Hui Yang, Peter Wilde, Ruijie Wang, Qi Meng, Hongyi Shi, Heng Yu, Zijun Zhou, Jianzhong Han, Weilin Liu

{"title":"Effect of Complexation with Different Molecular Weights of Oat β-Glucan and Sea Buckthorn Flavonoid on the Digestion of Rice Flour","authors":"Hui Yang, Peter Wilde, Ruijie Wang, Qi Meng, Hongyi Shi, Heng Yu, Zijun Zhou, Jianzhong Han, Weilin Liu","doi":"10.1021/acs.jafc.4c06405","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06405","url":null,"abstract":"The complex of oat β-glucan (OBG) and flavonoids hampered the digestion of starch-based food and retarded the blood glucose response; however, its effect on gastric emptying and its relative mechanism have not been thoroughly investigated. By using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), antioxidant ability, and enzymic inhibitory tests for the characterization and in vitro semi-dynamic digestion of complexes of OBG (high and low molecular weights) and sea buckthorn flavonoids, we found that the higher molecular weight complex (FU) exhibited stronger ABTS and DPPH radical scavenging abilities and higher α-glucosidase and α-amylase inhibition rates. Mice fed with rice flour with FU addition exhibited the slowest gastric emptying and intestinal propulsion rates and blood glucose rise and had the lowest activity of digestive enzymes and levels of insulin, ghrelin, motilin (MTL), and relevant gene (ghrelin and GHSR mRNA) expression than those in the control and low-molecular-weight groups. This study provided scientific data for the development of foods with delayed gastric rate and hypoglycemic index for specific populations.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Biotransformation of Volatile Markers During the Early Stage of Zygosaccharomyces rouxii and Zygosaccharomyces mellis Contamination in Acacia Honey. 金合欢蜂蜜中胭脂酵母菌和麦氏酵母菌污染初期挥发性标记物的鉴定和生物转化。

IF 5.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-11 DOI: 10.1021/acs.jafc.4c06157

Yuanyuan Huang, Jing Jin, Wei Cao, Yin Wang

{"title":"Identification and Biotransformation of Volatile Markers During the Early Stage of Zygosaccharomyces rouxii and Zygosaccharomyces mellis Contamination in Acacia Honey.","authors":"Yuanyuan Huang, Jing Jin, Wei Cao, Yin Wang","doi":"10.1021/acs.jafc.4c06157","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06157","url":null,"abstract":"To address the volatile markers and their biotransformation during the early stage of Zygosaccharomyces spp. contamination in acacia honey, headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and chemometric analyses were used to explore the variation of volatile compounds. A total of 36 and 35 volatile compounds were identified before and after contamination of Zygosaccharomyces rouxii and Zygosaccharomyces mellis, respectively. Methyl butyrate and 2-methyl-3-pentanone could be used as volatile markers of Z. rouxii and Z. mellis-contaminated honey, which were both specific products of the yeast's own fermentation. 2,5-Dimethylbenzaldehyde was identified as a volatile marker of Z. rouxii and Z. mellis-contaminated acacia honey, and it was a specific product resulting from the interaction of yeast and acacia honey. In addition, β-damascenone could be determined as a potential volatile marker after Z. rouxii-contaminated acacia honey. Methyl 2-methylbutyrate was used as a potential volatile marker in the high-concentration groups of Z. rouxii and Z. mellis. The content ranges of methyl butyrate, 2-methyl-3-pentanone, and 2,5-dimethylbenzaldehyde in four samples were 6.62-14.59, 3.15-5.42, and 52.52-215.19 μg/mL, respectively. The variation of volatile markers during the early stage of osmotolerant yeast contamination provided a theoretical basis for the use of HS-SPME-GC-MS for the rapid detection of acacia honey deterioration while reducing economic losses.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Whey N-Glycoproteins in Yak Colostrum and Mature Milk Based on 4D Label-Free Technology. 基于 4D 无标签技术的牦牛初乳和成熟乳中乳清 N-糖蛋白比较。

IF 5.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-11 DOI: 10.1021/acs.jafc.4c04354

Liwen Zhong, Yiheng Li, Jinliang Zhang, Pengjie Wang, Hao Zhang, Yingrui Lu, Li Song, Tao Peng, Jinxiu Ma, Weibing Zhang, Pengcheng Wen

{"title":"Comparison of Whey N-Glycoproteins in Yak Colostrum and Mature Milk Based on 4D Label-Free Technology.","authors":"Liwen Zhong, Yiheng Li, Jinliang Zhang, Pengjie Wang, Hao Zhang, Yingrui Lu, Li Song, Tao Peng, Jinxiu Ma, Weibing Zhang, Pengcheng Wen","doi":"10.1021/acs.jafc.4c04354","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c04354","url":null,"abstract":"Whey proteins are N-glycosylated proteins that play important roles in a variety of biological processes including immune defense. However, the N-glycosylation of yak colostrum (YC) and mature milk (YM) whey proteins is unknown. Therefore, this study systematically compared and analyzed YC and YM whey N-glycoproteomes using the 4D label-free technique. We identified 162 glycoproteins, 222 glycosylated peptides, and 234 glycosylation sites in YC and YM, of which 59 glycoproteins were differentially expressed in YC and YM. According to gene ontology annotation and KEGG pathway metabolism analysis, the differentially expressed N-glycoproteins were highly enriched in \"cell adhesion\", \"extracellular region\", and \"calcium binding\", and were mainly involved in the extracellular matrix (ECM)-receptor interaction pathway. The immunity-related N-glycoproteins, such as platelet glycoprotein 4 (CD36) and polymeric immunoglobulin receptor (PIGR), were observed to be different between YC and YM. The results revealed the glycosylation sites, composition, and biological functions of YC and YM whey N-glycoprotein, which supplemented our understanding of the N-glycosylation of yak whey proteins.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0