Journal of Agricultural and Food Chemistry最新文献

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Genome-Wide Association Study Reveals the Genetic Basis of Crude Fiber Components in Brassica napus L. Shoots at Stem Elongation Stage 全基因组关联研究揭示甘蓝型油菜茎伸长期嫩芽粗纤维成分的遗传基础
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2024-07-13 DOI: 10.1021/acs.jafc.4c03032
Rui Shi, Yu Cao, Tinghai Yang, Yaping Wang, Yanan Xiang, Feng Chen, Wei Zhang, Xiaoying Zhou, Chengming Sun, Sanxiong Fu, Maolong Hu, Jiefu Zhang, Xiaodong Wang
{"title":"Genome-Wide Association Study Reveals the Genetic Basis of Crude Fiber Components in Brassica napus L. Shoots at Stem Elongation Stage","authors":"Rui Shi, Yu Cao, Tinghai Yang, Yaping Wang, Yanan Xiang, Feng Chen, Wei Zhang, Xiaoying Zhou, Chengming Sun, Sanxiong Fu, Maolong Hu, Jiefu Zhang, Xiaodong Wang","doi":"10.1021/acs.jafc.4c03032","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c03032","url":null,"abstract":"<i>Brassica napus</i> is currently the principal field crop for producing materials for primary, secondary and tertiary industries. <i>B. napus</i> shoots at stem elongation stage are rich in anthocyanins, vitamin C and mineral elements such as selenium, calcium and zinc, and represent a new type of green vegetable. However, the high crude fiber (CF) content of <i>B. napus</i> shoots affects their taste, and few studies have focused on the quality traits of these vegetables. In this study, we investigated five traits related to the CF components, including neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), hemicellulose (Hem) and cellulose (Cel), of <i>B. napus</i> shoots. Whole-genome resequencing at a depth of ∼20× was utilized to genotype an association panel of 202 diverse accessions, which resulted in the identification of 6,093,649 single nucleotide polymorphisms (SNPs) and 996,252 indels, respectively. A genome-wide association study (GWAS) was performed for the five CF-related traits based on the phenotypic data observed in four environments. A total of 1,285 significant SNPs were detected at the threshold of -log<sub>10</sub> (<i>p</i>) = 5.16, and 97 significant association regions were obtained. In addition, seven candidate genes located on chromosomes A2 (one gene), A8 (three genes), A9 (two genes) and C9 (one gene) related to CF traits were identified, and ten lines containing low CF contents were selected as excellent germplasm resources for breeding. Our results contributed new insights into the genetic basis of CF traits and suggested germplasm resources for the quality improvement of <i>B. napus</i> shoots.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141602832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the Health Benefits of Boletus aereus Polysaccharides: Extraction, Structural Characterization, and Antiproliferative Properties against Non-Hodgkin's Lymphomas (NHLs). 探索牛肝菌多糖对健康的益处:牛肝菌多糖的提取、结构特征和对非霍奇金淋巴瘤(NHLs)的抗增殖特性。
IF 5.7 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2024-07-12 DOI: 10.1021/acs.jafc.4c03945
Haidi Wang, Linyu Yang, Qiang Qiu, Qianru Rao, Li Liu, Yuchen Cui, Liang Zhang, Yucheng Ma, Xi Jin, Dongxue Yang, Shiqian Qi, Kunjie Wang, Yong Li, Xufeng Zhang, Min Zhao
{"title":"Exploring the Health Benefits of <i>Boletus aereus</i> Polysaccharides: Extraction, Structural Characterization, and Antiproliferative Properties against Non-Hodgkin's Lymphomas (NHLs).","authors":"Haidi Wang, Linyu Yang, Qiang Qiu, Qianru Rao, Li Liu, Yuchen Cui, Liang Zhang, Yucheng Ma, Xi Jin, Dongxue Yang, Shiqian Qi, Kunjie Wang, Yong Li, Xufeng Zhang, Min Zhao","doi":"10.1021/acs.jafc.4c03945","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c03945","url":null,"abstract":"<p><p><i>Boletus aereus</i> Fr. ex Bull. stands out as a delectable edible mushroom with high nutritional and medicinal values, featuring polysaccharides as its primary nutrient composition. In our continuous exploration of its beneficial substances, a novel polysaccharide (BAPN-1) with a molecular weight of 2279 kDa was prepared. It was identified as a glucan with a backbone composed of the residues →4)-α-Glc<i>p</i>-(1→ and →4,6)-α-Glc<i>p</i>-(1→ connected in a proportion of 5:1 and a β-Glc<i>p</i>-(1→ side residue attached at C6 of the →4,6)-α-Glc<i>p</i>-(1→ residue. Biologically, BAPN-1 exhibited broad-spectrum antiproliferative activities against various NHL cells, including HuT-78, OCI-LY1, OCI-LY18, Jurkat, RL, and Karpas-299, with IC<sub>50</sub> values of 0.73, 1.21, 3.18, 1.52, 3.34, and 4.25 mg/mL, respectively. Additionally, BAPN-1 significantly induced cell cycle arrest in the G2/M phase and caused apoptosis of NHL cells. Mechanistically, bulk RNA sequencing and Western blot analysis revealed that BAPN-1 could upregulate cyclin B1 and enhance cleaved caspase-9 expression through the inhibition of FGFR3 and RAF-MEK-ERK signaling pathways. This work supports the improved utilization of <i>B. aereus</i> in high-value health products.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of Eurotium cristatum Fermented Thinned Young Apple and Mechanisms Underlying Its Alleviating Impacts on Experimental Colitis. 冠突散囊菌发酵变薄嫩苹果的特性及其缓解实验性结肠炎的机制
IF 5.7 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2024-07-12 DOI: 10.1021/acs.jafc.4c02005
Wei Song, Lanqi Zhou, Tianqi Liu, Guoze Wang, Jiayao Lv, Shiyi Zhang, Xiaoshuang Dai, Meng Wang, Lin Shi
{"title":"Characterization of <i>Eurotium cristatum</i> Fermented Thinned Young Apple and Mechanisms Underlying Its Alleviating Impacts on Experimental Colitis.","authors":"Wei Song, Lanqi Zhou, Tianqi Liu, Guoze Wang, Jiayao Lv, Shiyi Zhang, Xiaoshuang Dai, Meng Wang, Lin Shi","doi":"10.1021/acs.jafc.4c02005","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c02005","url":null,"abstract":"<p><p>A hundred million tons of young apples are thinned and discarded in the orchard per year, aiming to increase the yield and quality of apples. We fermented thinned young apples using a potential probiotic fungus, <i>Eurotium cristatum</i>, which notably disrupted the microstructure of raw samples, as characterized by the scanning electron microscope. Fermentation substantially altered the metabolite profiles of samples, which are predicted to alleviate colitis <i>via</i> regulating inflammatory response and response to lipopolysaccharide by using network pharmacology analysis. <i>In vivo</i>, oral gavage of water extracts of <i>E. cristatum</i> fermented young apples (<i>E</i>.YAP) effectively alleviated DSS-induced colitis, restored the histopathology damage, reduced the levels of inflammatory cytokines, and promoted colonic expressions of tight junction proteins. Moreover, <i>E</i>.YAP ameliorated gut dysbacteriosis by increasing abundances of <i>Lactobacillus</i>,<i>Blautia</i>, <i>Muribaculaceae</i>, and <i>Prevotellaceae_UCG-001</i> while inhibiting <i>Turicibacter</i>, <i>Alistipes</i>, and <i>Desulfovibrio</i>. Importantly, <i>E</i>.YAP increased colonic bile acids, such as CA, TCA, DCA, TUDCA, and LCA, thereby alleviating colitis <i>via</i> PXR/NF-κB signaling. Furthermore, a synbiotic combination with <i>Limosilactobacillus reuteri</i> WX-94, a probiotic strain isolated from feces of healthy individuals with anti-inflammatory properties, augmented anticolitis capacities of <i>E</i>.YAP. Our findings demonstrate that <i>E</i>.YAP could be a novel, potent, food-based anti-inflammatory prebiotic for relieving inflammatory injuries.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141597914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bta-miR-200a Regulates Milk Fat Biosynthesis by Targeting IRS2 to Inhibit the PI3K/Akt Signal Pathway in Bovine Mammary Epithelial Cells. Bta-miR-200a 通过靶向 IRS2 抑制牛乳腺上皮细胞中的 PI3K/Akt 信号通路来调节乳脂的合成。
IF 5.7 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2024-07-12 DOI: 10.1021/acs.jafc.4c02508
Jianbing Tan, Benshun Yang, Liang Qiu, Ruiying He, Zhangqing Wu, Miaomiao Ye, Linsen Zan, Wucai Yang
{"title":"Bta-miR-200a Regulates Milk Fat Biosynthesis by Targeting <i>IRS2</i> to Inhibit the PI3K/Akt Signal Pathway in Bovine Mammary Epithelial Cells.","authors":"Jianbing Tan, Benshun Yang, Liang Qiu, Ruiying He, Zhangqing Wu, Miaomiao Ye, Linsen Zan, Wucai Yang","doi":"10.1021/acs.jafc.4c02508","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c02508","url":null,"abstract":"<p><p>Milk fat synthesis has garnered significant attention due to its influence on the quality of milk. Recently, an increasing amount of proofs have elucidated that microRNAs (miRNAs) are important post-transcriptional factor involved in regulating gene expression and play a significant role in milk fat synthesis. MiR-200a was differentially expressed in the mammary gland tissue of dairy cows during different lactation periods, which indicated that miR-200a was a candidate miRNA involved in regulating milk fat synthesis. In our research, we investigated the potential function of miR-200a in regulating milk fat biosynthesis in bovine mammary epithelial cells (BMECs). We discovered that miR-200a inhibited cellular triacylglycerol (TAG) synthesis and suppressed lipid droplet formation; at the same time, miR-200a overexpression suppressed the mRNA and protein expression of milk fat metabolism-related genes, such as fatty acid synthase (<i>FASN</i>), peroxisome proliferator-activated receptor gamma (<i>PPARγ</i>), sterol regulatory element-binding protein 1 (<i>SREBP1</i>), CCAAT enhancer binding protein alpha (<i>CEBPα</i>), etc. However, knocking down miR-200a displayed the opposite results. We uncovered that insulin receptor substrate 2 (<i>IRS2</i>) was a candidate target gene of miR-200a through the bioinformatics online program TargetScan. Subsequently, it was confirmed that miR-200a directly targeted the 3'-untranslated region (3'-UTR) of <i>IRS2</i> via real-time fluorescence quantitative PCR (RT-qPCR), western blot analysis, and dual-luciferase reporter gene assay. Additionally, IRS2 knockdown in BMECs has similar effects to miR-200a overexpression. Our research set up the mechanism by which miR-200a interacted with <i>IRS2</i> and discovered that miR-200a targeted <i>IRS2</i> and modulated the activity of the PI3K/Akt signaling pathway, thereby taking part in regulating milk fat synthesis in BMECs. Our research results provided valuable information on the molecular mechanisms for enhancing milk quality from the view of miRNA-mRNA regulatory networks.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141597887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regulatory Peptide Encoded by the Primary Transcript of miR396a Influences Gene Expression and Root Development in Solanum lycopersicum. miR396a 主转录本编码的调控肽影响茄果类植物的基因表达和根系发育
IF 5.7 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2024-07-12 DOI: 10.1021/acs.jafc.4c03588
Zhengjie Wang, Ruili Lv, Chenglin Su, Yan Li, Sizhe Fang, Ruirui Yang, Jiaxuan Zhu, Ruiming Wang, Jun Meng, Yushi Luan
{"title":"Regulatory Peptide Encoded by the Primary Transcript of miR396a Influences Gene Expression and Root Development in <i>Solanum lycopersicum</i>.","authors":"Zhengjie Wang, Ruili Lv, Chenglin Su, Yan Li, Sizhe Fang, Ruirui Yang, Jiaxuan Zhu, Ruiming Wang, Jun Meng, Yushi Luan","doi":"10.1021/acs.jafc.4c03588","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c03588","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) are the processing products of primary miRNAs (pri-miRNAs) that regulate the expression of target genes. Recent studies have demonstrated that some pri-miRNAs can encode small peptides (miPEPs) that perform significant biological functions. The function of miPEPs in tomatoes, an important model horticultural crop, remains to be investigated. Here, we characterized the primary sequence of tomato miR396a using 5' RACE and confirmed the presence of miPEP396a in tomato by verifying the translational activity of the start codon. It primarily resides in the nucleus to exert its function and additionally regulates the expression of pri-miR396a, miR396a, and its target genes. Transcriptomic and metabolomic analyses showed that <i>in vitro</i> synthesis of miPEP396a significantly increased the expression of genes related to phenylpropanoid biosynthesis and hormones in tomato. Meanwhile, our <i>in vitro</i> application of miPEP396a in tomato significantly inhibited the elongation of tomato primary roots. In conclusion, our results indicate that miPEP396a regulates root growth in tomato by specifically promoting miR396a expression, provide insight into the function of miPEPs in tomato and potential applications.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cadmium Maximum Levels and Residue Situation in the German Wheat and Rye Harvest from 1975 to 2021. 1975 年至 2021 年德国小麦和黑麦收成中的镉最高含量和残留情况。
IF 5.7 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2024-07-12 DOI: 10.1021/acs.jafc.4c03560
Alexandra Hüsken, Lidia Arent, Regina Lohmayer
{"title":"Cadmium Maximum Levels and Residue Situation in the German Wheat and Rye Harvest from 1975 to 2021.","authors":"Alexandra Hüsken, Lidia Arent, Regina Lohmayer","doi":"10.1021/acs.jafc.4c03560","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c03560","url":null,"abstract":"<p><p>For a better understanding of cadmium (Cd) accumulation over long time periods in cereals, Cd levels of the German wheat and rye harvest from 1975 to 2021 were analyzed. Overall, wheat had higher grain Cd concentrations than rye. Comparing mean values from different time periods showed that Cd levels in winter rye have stabilized, while Cd concentrations in winter wheat have decreased. Furthermore, Cd concentrations in almost all samples were below the newly introduced European Commission limits specifying the maximum permissible contaminant levels in foodstuffs (Cd in grains: rye 50 μg/kg FW; wheat 100 μg/kg FW). However, it is important to note that Cd is still ubiquitous in the German wheat and rye harvest. Although there has been a significant reduction in emissions and imissions for around 30 years, the extraordinarily long biological half-life and carcinogenicity of Cd still make it a relevant substance to food safety and human health.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141597888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polyunsaturated Fatty Acids in Quinoa Induce Ferroptosis of Colon Cancer by Suppressing Stemness. 藜麦中的多不饱和脂肪酸通过抑制干性诱导结肠癌的铁变态反应
IF 5.7 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2024-07-11 DOI: 10.1021/acs.jafc.4c00118
Songtao Li, Man Ding, Mangmang Feng, Xiaxia Fan, Zhuoyu Li
{"title":"Polyunsaturated Fatty Acids in Quinoa Induce Ferroptosis of Colon Cancer by Suppressing Stemness.","authors":"Songtao Li, Man Ding, Mangmang Feng, Xiaxia Fan, Zhuoyu Li","doi":"10.1021/acs.jafc.4c00118","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c00118","url":null,"abstract":"<p><p>Polyunsaturated fatty acids (PUFAs) are essential nutrients for the human body, playing crucial roles in reducing blood lipids, anti-inflammatory responses, and anticancer effect. Quinoa is a nutritionally sound food source, rich in PUFAs. This study investigates the role of quinoa polyunsaturated fatty acids (QPAs) on quelling drug resistance in colorectal cancer. The results reveal that QPA downregulates the expression of drug-resistant proteins P-gp, MRP1, and BCRP, thereby enhancing the sensitivity of colorectal cancer drug-resistant cells to the chemotherapy drug. QPA also inhibits the stemness of drug-resistant colorectal cancer cells by reducing the expression of the stemness marker CD44. Consequently, it suppresses the downstream protein SLC7A11 and leads to ferroptosis. Additionally, QPA makes the expression of ferritin lower and increases the concentration of free iron ions within cells, leading to ferroptosis. Overall, QPA has the dual-function reversing drug resistance in colorectal cancer by simultaneously inhibiting stemness and inducing ferroptosis. This study provides a new option for chemotherapy sensitizers and establishes a theoretical foundation for the development and utilization of quinoa.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
β-Sitosterol Inhibits Tumor Growth and Amplifies Rituximab Sensitivity through Acid Sphingomyelinase/Ceramide Signaling in Diffuse Large B-Cell Lymphoma β-谷甾醇在弥漫大 B 细胞淋巴瘤中通过酸性鞘磷脂酶/神经酰胺信号传导抑制肿瘤生长并增强利妥昔单抗的敏感性
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2024-07-11 DOI: 10.1021/acs.jafc.4c00014
Guoping He, Minghan Qiu, Zhen Yang, Ke Zhao, Ruxue Liu, Hanwei Mei, Xuanzhu Zhao, Teng Song, Xiaozhi Liu, Miao Zhang, Huaqing Wang
{"title":"β-Sitosterol Inhibits Tumor Growth and Amplifies Rituximab Sensitivity through Acid Sphingomyelinase/Ceramide Signaling in Diffuse Large B-Cell Lymphoma","authors":"Guoping He, Minghan Qiu, Zhen Yang, Ke Zhao, Ruxue Liu, Hanwei Mei, Xuanzhu Zhao, Teng Song, Xiaozhi Liu, Miao Zhang, Huaqing Wang","doi":"10.1021/acs.jafc.4c00014","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c00014","url":null,"abstract":"Rituximab (RTX) resistance is a notable challenge in treating diffuse large B-cell lymphoma (DLBCL). β-Sitosterol (β-ST) is a plant sterol that has been found in a broad variety of fruits, spices, and medicinal plants. The antineoplastic properties of β-ST are established in various solid malignancies; however, its effect on DLBCL is uncharted. This study investigates the role of β-ST in DLBCL as well as the underlying mechanisms. Our findings indicated that β-ST impeded DLBCL cell proliferation in a concentration- and time-dependent manner. β-ST appeared to alter sphingolipid metabolism, facilitate acid sphingomyelinase (ASM) translocation to the plasma membrane, augment ceramide platforms through increased ceramide synthesis, and consequently induce apoptosis in DLBCL cells. Furthermore, we found that RTX initiated both apoptotic and survival pathways <i>in vitro</i>, with the former contingent on the transient activation of the ASM, and β-ST could amplify the anti-DLBCL efficacy of RTX by modulating ASM/Ceramide (Cer) signaling. Collectively, our findings elucidate the mechanistic role of β-ST in DLBCL and underscore its potential in amplifying the antineoplastic efficacy of RTX via ASM activation, proposing a potential avenue to improve the efficacy of RTX therapy.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tangeretin Enhances Muscle Endurance and Aerobic Metabolism in Mice via Targeting AdipoR1 to Increase Oxidative Myofibers 橘皮苷通过靶向 AdipoR1 增加氧化肌纤维增强小鼠肌肉耐力和有氧代谢
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2024-07-11 DOI: 10.1021/acs.jafc.3c09386
Jinjie Li, Jiangtao Li, Amin Ullah, Xiaoyang Shi, Xinyuan Zhang, Zhenwei Cui, Quanjun Lyu, Guangning Kou
{"title":"Tangeretin Enhances Muscle Endurance and Aerobic Metabolism in Mice via Targeting AdipoR1 to Increase Oxidative Myofibers","authors":"Jinjie Li, Jiangtao Li, Amin Ullah, Xiaoyang Shi, Xinyuan Zhang, Zhenwei Cui, Quanjun Lyu, Guangning Kou","doi":"10.1021/acs.jafc.3c09386","DOIUrl":"https://doi.org/10.1021/acs.jafc.3c09386","url":null,"abstract":"Slow oxidative myofibers play an important role in improving muscle endurance performance and maintaining body energy homeostasis. However, the targets and means to regulate slow oxidative myofibers proportion remain unknown. Here, we show that tangeretin (TG), a natural polymethoxylated flavone, significantly activates slow oxidative myofibers-related gene expression and increases type I myofibers proportion, resulting in improved endurance performance and aerobic metabolism in mice. Proteomics, molecular dynamics, cellular thermal shift assay (CETSA) and drug affinity responsive target stability (DARTS) investigations revealed that TG can directly bind to adiponectin receptor 1 (AdipoR1). Using AdipoR1-knockdown C2C12 cells and muscle-specific AdipoR1-knockout mice, we found that the positive effect of TG on regulating slow oxidative myofiber related markers expression is mediated by AdipoR1 and its downstream AMPK/PGC-1α pathway. Together, our data uncover TG as a natural compound that regulates the identity of slow oxidative myofibers via targeting the AdipoR1 signaling pathway. These findings further unveil the new function of TG in increasing the proportion of slow oxidative myofibers and enhancing skeletal muscle performance.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Salidroside Alleviates Furan-Induced Impaired Gut Barrier and Inflammation via Gut Microbiota–SCFA–TLR4 Signaling 水杨甙通过肠道微生物群-SCFA-TLR4 信号传导缓解呋喃引起的肠道屏障受损和炎症反应
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2024-07-11 DOI: 10.1021/acs.jafc.4c02433
Ziyue Wang, Lu Li, Wenliang Li, Haiyang Yan, Yuan Yuan
{"title":"Salidroside Alleviates Furan-Induced Impaired Gut Barrier and Inflammation via Gut Microbiota–SCFA–TLR4 Signaling","authors":"Ziyue Wang, Lu Li, Wenliang Li, Haiyang Yan, Yuan Yuan","doi":"10.1021/acs.jafc.4c02433","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c02433","url":null,"abstract":"As a food contaminant that can be quickly absorbed through the gastrointestinal system, furan has been shown to disrupt the intestinal flora and barrier. Investigation of the intestinal toxicity mechanism of furan is of great significance to health. We previously identified the regulatory impact of salidroside (SAL) against furan-provoked intestinal damage, and the present work further explored whether the alleviating effect of SAL against furan-caused intestinal injury was based on the intestinal flora; three models, normal, pseudo-germ-free, and fecal microbiota transplantation (FMT), were established, and the changes in intestinal morphology, barrier, and inflammation were observed. Moreover, 16S rDNA sequencing observed the variation of the fecal flora associated with inflammation and short-chain fatty acids (SCFAs). Results obtained from the LC–MS/MS suggested that SAL increased furan-inhibited SCFA levels, activated the mRNA expressions of SCFA receptors (<i>GPR41</i>, <i>GPR43</i>, and <i>GPR109A</i>), and inhibited the furan-activated TLR4/MyD88/NF-κB signaling. Analysis of protein–protein interaction further confirmed the aforementioned effects of SAL, which inhibited furan-induced barrier damage and intestinal inflammation.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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