Journal of Agricultural and Food Chemistry最新文献

Kiwifruit Polysaccharides Alleviate Ulcerative Colitis via Regulating Gut Microbiota-Dependent Tryptophan Metabolism and Promoting Colon Fucosylation 猕猴桃多糖通过调节肠道微生物群依赖性色氨酸代谢和促进结肠岩藻糖基化缓解溃疡性结肠炎

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-21 DOI: 10.1021/acs.jafc.4c06435

Yixiao Li, Yanhui Han, Xuejie Wang, Xingbin Yang, Daoyuan Ren

{"title":"Kiwifruit Polysaccharides Alleviate Ulcerative Colitis via Regulating Gut Microbiota-Dependent Tryptophan Metabolism and Promoting Colon Fucosylation","authors":"Yixiao Li, Yanhui Han, Xuejie Wang, Xingbin Yang, Daoyuan Ren","doi":"10.1021/acs.jafc.4c06435","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06435","url":null,"abstract":"A previous study showed that kiwifruit polysaccharide (KFP) has benefits in relieving intestinal inflammation, while the underlying mechanism remains unresolved. The objective of this study was to investigate the regulatory effect of KFP on the gut microbiota metabolism and intestinal barrier of ulcerative colitis (UC) mice induced by dextran sulfate sodium (DSS). KFP significantly improved the UC symptoms including weight loss, shortened colon length, splenomegaly, diarrhea, hematochezia, and colon inflammation of mice. In addition, KFP could alleviate DSS-caused gut microbiota dysbiosis and increase the levels of short-chain fatty acids in the cecal contents of mice. Furthermore, the results of nontargeted and targeted metabolomics analysis combined with antibiotic treatment revealed that KFP could regulate gut microbiota-dependent tryptophan metabolism, activate the aryl hydrocarbon receptor (AhR) in colon cells, and enhance interleukin-22 production and tight junction proteins' (ZO-1, occludin, and claudin3) expression to repair the intestinal barrier in UC mice. Immunofluorescence results showed that KFP significantly upregulated the conjunction of lectin WGA and UEA1 in the UC mouse colon, implying that KFP promoted fucosylation in the colon. These results suggest that KFP alleviates UC primarily via targeting the gut microbiota involved in the AhR pathway and upregulating colon fucosylation.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142452088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isomalto-Oligosaccharide Potentiates Alleviating Effects of Intermittent Fasting on Obesity-Related Cognitive Impairment during Weight Loss and the Rebound Weight Gain 异麦芽低聚糖能增强间歇性禁食对减肥和体重反弹期间肥胖相关认知障碍的缓解作用

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-21 DOI: 10.1021/acs.jafc.4c07351

Tianqi Liu, Lanqi Zhou, Rui Dong, Yizhe Qu, Yuan Liu, Wei Song, Jiayao Lv, Shan Wu, Wen Peng, Lin Shi

{"title":"Isomalto-Oligosaccharide Potentiates Alleviating Effects of Intermittent Fasting on Obesity-Related Cognitive Impairment during Weight Loss and the Rebound Weight Gain","authors":"Tianqi Liu, Lanqi Zhou, Rui Dong, Yizhe Qu, Yuan Liu, Wei Song, Jiayao Lv, Shan Wu, Wen Peng, Lin Shi","doi":"10.1021/acs.jafc.4c07351","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07351","url":null,"abstract":"Obesity-related cognitive dysfunction poses a significant threat to public health. The present study demonstrated mitigating effects of intermittent fasting (IF) and its combination with isomalto-oligosaccharides and IF (IF + IMO) on cognitive impairments induced by a high-fat-high-fructose (HFHF) diet in mice, with IF + IMO exhibiting superior effects. Transcriptomic analysis of the hippocampus revealed that the protective effects on cognition might be attributed to the suppression of toll-like receptor 4 (TLR4)/NFκB signaling, oxidative phosphorylation, and neuroinflammation. Moreover, both IF and IF + IMO modulated the gut microbiome and promoted the production of short-chain fatty acids, with IF + IMO displaying more pronounced effects. IF + IMO-modulated gut microbiota, metabolites, and molecular targets associated with cognitive impairments were further corroborated using human data from public databases Gmrepo and gutMgene. Furthermore, the fecal microbiome transplantation confirmed the direct impacts of IF + IMO-derived microbiota on improving cognition functions by suppressing TLR4/NFκB signaling and increasing BDNF levels. Notably, prior exposure to IF + IMO prevented weight-regain-induced cognitive decline, suppressed TLR4/NFκB signaling and inflammatory cytokines in the hippocampus, and mitigated weight regain-caused gut dysbacteriosis without altering body weight. Our study underscores that IMO-augmented alleviating effects of IF on obesity-related cognitive impairment particularly during weight-loss and weight-regain periods, presenting a novel nutritional strategy to tackle obesity-related neurodegenerative disorders.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142452084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactivity-Oriented Separation of “Pepper Alkaloids” from Piper sintenense Hatusima with Potential Antigouty Arthritis Activity 以生物活性为导向分离具有潜在抗痛风性关节炎活性的 Piper sintenense Hatusima 中的 "胡椒生物碱"

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-21 DOI: 10.1021/acs.jafc.4c05999

Zhiren Yao, Tian Li, Meiling Jin, Siyang Fang, Jie Wang, Jiaming Zheng, Ke Pan, Jian Zhang, Guoqing Zhao, Zhiqi Yin, Yaping Huang

{"title":"Bioactivity-Oriented Separation of “Pepper Alkaloids” from Piper sintenense Hatusima with Potential Antigouty Arthritis Activity","authors":"Zhiren Yao, Tian Li, Meiling Jin, Siyang Fang, Jie Wang, Jiaming Zheng, Ke Pan, Jian Zhang, Guoqing Zhao, Zhiqi Yin, Yaping Huang","doi":"10.1021/acs.jafc.4c05999","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c05999","url":null,"abstract":"Piper sintenense Hatusima (PsH) is a member of the Piper genus used as food and folk medicine in China. However, the detailed chemical ingredients and potential pharmacological effects are still underexploited. In this study, we evaluated the anti-inflammatory and antigouty arthritis effect of the ethanolic extract of P. sintenense. Active compounds were isolated from the petroleum ether fraction resulting in six novel amide alkaloids (1–6) and 13 known analogues (7–19). All of the compounds exhibited excellent anti-inflammatory activities. Bioinformatics analysis and in vitro experiments showed that compound 4 had anti-inflammatory properties (IC50 = 4.86 ± 0.32 μM) and antigouty arthritis activity. Mechanistic studies revealed that compound 4 exerted its favorable efficacy by regulating macrophage polarization by inhibiting P-STAT1/4. These findings provide new insights into the chemical composition and function of P. sintenense and expand its promising application as a functional food in the prevention and treatment of gouty arthritis.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142452087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for the Quercetin Binding Site of Glycogen Phosphorylase as a Target for Liver-Isoform-Selective Inhibitors against Glioblastoma: Investigation of Flavanols Epigallocatechin Gallate and Epigallocatechin 证明糖原磷酸化酶的槲皮素结合位点是针对胶质母细胞瘤的肝异构体选择性抑制剂的靶点：对黄烷醇表没食子儿茶素没食子酸酯和表没食子儿茶素的研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-21 DOI: 10.1021/acs.jafc.4c06920

Serafeim Alexopoulos, Megan McGawley, Roshini Mathews, Souzana Papakostopoulou, Symeon Koulas, Demetres D. Leonidas, Tamara Zwain, Joseph M. Hayes, Vasiliki Skamnaki

{"title":"Evidence for the Quercetin Binding Site of Glycogen Phosphorylase as a Target for Liver-Isoform-Selective Inhibitors against Glioblastoma: Investigation of Flavanols Epigallocatechin Gallate and Epigallocatechin","authors":"Serafeim Alexopoulos, Megan McGawley, Roshini Mathews, Souzana Papakostopoulou, Symeon Koulas, Demetres D. Leonidas, Tamara Zwain, Joseph M. Hayes, Vasiliki Skamnaki","doi":"10.1021/acs.jafc.4c06920","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06920","url":null,"abstract":"Glycogen phosphorylase (GP) is the rate-determining enzyme in glycogenolysis, and its druggability has been extensively studied over the years for the development of therapeutics against type 2 diabetes (T2D) and, more recently, cancer. However, the conservation of binding sites between the liver and muscle isoforms makes the inhibitor selectivity challenging. Using a combination of kinetic, crystallographic, modeling, and cellular studies, we have probed the binding of dietary flavonoids epigallocatechin gallate (EGCG) and epigallocatechin (EGC) to GP isoforms. The structures of rmGPb-EGCG and rmGPb-EGC complexes were determined by X-ray crystallography, showing binding at the quercetin binding site (QBS) in agreement with kinetic studies that revealed both compounds as noncompetitive inhibitors of GP, with EGCG also causing a significant reduction in cell viability and migration of U87-MG glioblastoma cells. Interestingly, EGCG exhibits different binding modes to GP isoforms, revealing QBS as a promising site for GP targeting, offering new opportunities for the design of liver-selective GP inhibitors.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142452089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Saccharomyces cerevisiae for the Production of Punicic Acid-Rich Yeast Biomass 对酿酒酵母进行工程改造以生产富含丁酸的酵母生物质

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-21 DOI: 10.1021/acs.jafc.4c08252

Juli Wang, Guanqun Chen

引用次数: 0

Nanomaterial-Based Biofortification: Potential Benefits and Impacts of Crops 基于纳米材料的生物强化：作物的潜在益处和影响

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-21 DOI: 10.1021/acs.jafc.4c05079

Erico R. Carmona, Cynthia Rojo, Víctor Vergara Carmona

引用次数: 0

Overexpression of ALDOC Promotes Porcine Intramuscular and Intermuscular Fat Deposition by Activating the AKT-mTORC1 Signaling Pathway 过表达 ALDOC 可通过激活 AKT-mTORC1 信号通路促进猪肌内和肌间脂肪沉积

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-20 DOI: 10.1021/acs.jafc.4c07781

Zeqiang Ma, Yingqian Wang, Junnan Shen, Wenyong Yan, Chaoyue Cao, Ming Feng, Jiahua Zhu, Weijun Pang

{"title":"Overexpression of ALDOC Promotes Porcine Intramuscular and Intermuscular Fat Deposition by Activating the AKT-mTORC1 Signaling Pathway","authors":"Zeqiang Ma, Yingqian Wang, Junnan Shen, Wenyong Yan, Chaoyue Cao, Ming Feng, Jiahua Zhu, Weijun Pang","doi":"10.1021/acs.jafc.4c07781","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07781","url":null,"abstract":"Skeletal muscle fat accumulation, a common complication of obesity, has garnered increasing attention. For livestock animals, intramuscular fat content is a key determinant of meat quality and directly influences consumer preferences for different meat products. However, genes effectively regulating intramuscular and intermuscular fat (IIMF) deposition remain largely unexplored. To address this gap, we employed transcriptome sequencing of muscle from pigs with high and low IIMF contents, uncovering Aldolase C (ALDOC) as a novel key gene controlling IIMF. ALDOC overexpression significantly enhanced the IIMF content both in vivo and in vitro. Mechanistic investigations revealed that ALDOC activates the AKT-mTORC1 axis to promote lipid accumulation in myotubes and intramuscular adipocytes. Notably, the mTORC1 inhibitor rapamycin abrogated ALDOC’s proadipogenic effect. Our findings establish ALDOC as a novel key gene regulating IIMF deposition and identify the ALDOC-AKT-mTORC1 signaling pathway as a promising therapeutic target for treating skeletal muscle fat infiltration and improving pork quality.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethanolic Extract of Bergenia purpurascens Exhibits Antimelanogenic Effects in B16F10 Cells through Multiple Mechanisms That Suppress Tyrosinase 紫花地丁乙醇提取物通过抑制酪氨酸酶的多重机制对 B16F10 细胞产生抗黑色素生成作用

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-19 DOI: 10.1021/acs.jafc.4c05392

Jaeseong Seo, Youn-Hwan Hwang, Ami Lee, Kyoung mi Moon, Ji Yun Van, Hyeon Hak Jeong, Heeyeon Ryu, Hui Peng Xi, Bonggi Lee

{"title":"Ethanolic Extract of Bergenia purpurascens Exhibits Antimelanogenic Effects in B16F10 Cells through Multiple Mechanisms That Suppress Tyrosinase","authors":"Jaeseong Seo, Youn-Hwan Hwang, Ami Lee, Kyoung mi Moon, Ji Yun Van, Hyeon Hak Jeong, Heeyeon Ryu, Hui Peng Xi, Bonggi Lee","doi":"10.1021/acs.jafc.4c05392","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c05392","url":null,"abstract":"Bergenia purpurascens, renowned for its antibacterial and antioxidant properties, remains relatively unexplored in its impact on melanogenesis. This study delves into the antimelanogenic potential of the ethanol extract derived from B. purpurascens (BPE). Our investigations reveal the robust antioxidant capabilities of the BPE, along with its effective inhibition of mushroom tyrosinase activity. Remarkably, these effects were significantly stronger than those observed with arbutin, the positive control. In vitro assays demonstrate the BPE’s efficacy in reducing the melanin content and tyrosinase activity in α-MSH-stimulated B16F10 cells. Immunofluorescence and qPCR analyses further reveal the BPE’s ability to inhibit MITF-mediated gene expression levels associated with melanogenesis, including Trp-1, Trp-2, and tyrosinase. These findings suggest that the extract operates through dual mechanisms, suppressing both tyrosinase activity and key transcription factor-mediated downstream signaling. Utilizing UPLC-MS/MS analysis, we identified six key compounds implicated in tyrosinase activity inhibition and melanogenesis suppression. Docking simulations confirm moderate binding affinities between these compounds and tyrosinase. This study highlights the potential of B. purpurascens as a novel natural agent for depigmentation in medicinal and cosmetic applications, elucidating its dual mechanism of action in melanogenesis inhibition.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142450036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary and Expression Analyses of the bZIP Family in Tea Plants (Camellia sinensis) and Functional Characterization of CsbZIP3/42/6 in Response to Environmental Stresses 茶树（Camellia sinensis）中 bZIP 家族的进化和表达分析以及 CsbZIP3/42/6 在环境胁迫下的功能特性分析

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-19 DOI: 10.1021/acs.jafc.4c06725

Man Zhang, Yanhui Liu, Jianlong Li, Bo Zhou, Yiyong Chen, Hao Tang, Yingying Cui, Jiayu Liu, Jinchi Tang

{"title":"Evolutionary and Expression Analyses of the bZIP Family in Tea Plants (Camellia sinensis) and Functional Characterization of CsbZIP3/42/6 in Response to Environmental Stresses","authors":"Man Zhang, Yanhui Liu, Jianlong Li, Bo Zhou, Yiyong Chen, Hao Tang, Yingying Cui, Jiayu Liu, Jinchi Tang","doi":"10.1021/acs.jafc.4c06725","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06725","url":null,"abstract":"Basic leucine zipper (bZIP) transcription factors play crucial roles in various biological processes and responses to environmental stresses. However, the functions of the bZIP family in tea plants remain largely unexplored. Here, we identified 74 bZIP genes in tea plants (Camellia sinensis) and classified them into 12 phylogenetic groups, supported by analyses of conserved motifs and gene structures. Cis-element analysis provided insights into the potential roles of CsbZIP genes in phytohormone signaling and stress responses. Tissue-specific expression analysis demonstrated differential expression profiles of CsbZIP genes, suggesting their tissue- and stage-specific functions. Additionally, varying expression levels under different abiotic stresses indicated functional divergence of the CsbZIP family during the long-term evolution. Notably, CsbZIP3/42/6 were identified as positive regulators of drought and salt stress responses but negative regulators in response to pathogen infection, and CsbZIP42 could interact with CsbZIP3 and CsbZIP6 in regulating these environmental stresses. This study provides valuable information on potential applications for improving stress tolerance and overall plant health of tea plants.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Diosgenin Reduces Acute Kidney Injury and Ameliorates the Progression to Chronic Kidney Disease by Modifying the NOX4/p65 Signaling Pathways” 对 "薯蓣皂苷通过改变 NOX4/p65 信号通路减轻急性肾损伤并改善慢性肾病的进展 "的更正

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-10-19 DOI: 10.1021/acs.jafc.4c09385

Chih-Hung Chiang, Tien-Yun Lan, Jung-Hung Hsieh, Su-Chu Lin, Jaw-Wen Chen, Ting-Ting Chang

引用次数: 0