Journal of Agricultural and Food Chemistry最新文献

Selection and Application of an Aptamer for the Extraction and HPLC-MS/MS Determination of Six Perfluorinated Compounds in Food 食品中六种全氟化合物提取及HPLC-MS/MS测定适配体的选择与应用

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-11 DOI: 10.1021/acs.jafc.6c06107

Shengyu You,Huning Chen,Qinyan Xu,Xiaoyao Wang,Zepeng Gu,Zhouping Wang,Nuo Duan,Shijia Wu

引用次数: 0

Microbial Bioprocessing of Plant-Based Food Matrices: From Conventional Analysis to In Situ Characterization of Cell Wall-Bound Phenolic Release 植物性食物基质的微生物处理：从常规分析到细胞壁结合酚释放的原位表征

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-11 DOI: 10.1021/acs.jafc.6c00824

Yuhao Li,Jiapeng Li,Weiwei He,Ningyu Lei,Xin Wang,Yangyang Xun,Shuai Liu,Xiaoyi Hu,Xiaoxiao Song,Shaoping Nie,Junyi Yin,Mingyong Xie

{"title":"Microbial Bioprocessing of Plant-Based Food Matrices: From Conventional Analysis to In Situ Characterization of Cell Wall-Bound Phenolic Release","authors":"Yuhao Li,Jiapeng Li,Weiwei He,Ningyu Lei,Xin Wang,Yangyang Xun,Shuai Liu,Xiaoyi Hu,Xiaoxiao Song,Shaoping Nie,Junyi Yin,Mingyong Xie","doi":"10.1021/acs.jafc.6c00824","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c00824","url":null,"abstract":"The release mechanisms of bound phenolics (BPs) from plant cell walls during bioprocessing, such as microbial fermentation, are highly dynamic and exhibit microscopic spatial selectivity. While conventional chemical analysis can provide macroscopic data on BPs release, it fails to reveal the microscopic spatial heterogeneity and causal relationships underlying the cell wall degradation process. This review examines both conventional extraction-based analytical methods and emerging in situ imaging technologies, such as Raman/FTIR spectroscopy, confocal laser scanning microscopy, immunofluorescence, and mass spectrometry imaging, highlighting their complementary roles and current technical bottlenecks in characterizing BPs release and cell wall degradation. Furthermore, a unified multiscale, multimodal framework is proposed, integrating high-resolution imaging, dynamic fluorescence probes, multidimensional spectroscopy, and machine-learning-assisted data fusion. This holistic characterization strategy elucidates the precise mechanisms governing phenolic release, providing a theoretical basis to guide the targeted enrichment of BPs and advance the efficient utilization of plant biomass and agricultural residues.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"20 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149374748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute Total Ammonia Nitrogen Exposure in Pacific White Shrimp (Litopenaeus vannamei): Physiological and Multi-Omics Responses Associated with Tissue Semicarbazide 凡纳滨对虾急性总氨氮暴露：与组织氨基脲相关的生理和多组学反应

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-10 DOI: 10.1021/acs.jafc.6c10606

Liwei Long,Siqi Du,Zhi Li,Yaxin Sang,Wenlong Yu,Xianghong Wang

引用次数: 0

A Novel α2,3-Sialyltransferase Enabling the Synthesis of para α2,3-Sialylated Human Milk Oligosaccharides via Immobilized Cascade Enzymes 一种新型α2,3-唾液基转移酶可通过固定化级联酶合成对α2,3-唾液基化的人乳低聚糖

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-10 DOI: 10.1021/acs.jafc.6c12083

Rui Chen,Yifan Cao,Zhuoning Cao,Xiaomeng Wang,Kunpeng Gao,Mengxiao Li,Hong Jiang,Xiangzhao Mao

{"title":"A Novel α2,3-Sialyltransferase Enabling the Synthesis of para α2,3-Sialylated Human Milk Oligosaccharides via Immobilized Cascade Enzymes","authors":"Rui Chen,Yifan Cao,Zhuoning Cao,Xiaomeng Wang,Kunpeng Gao,Mengxiao Li,Hong Jiang,Xiangzhao Mao","doi":"10.1021/acs.jafc.6c12083","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c12083","url":null,"abstract":"Sialylated oligosaccharides are critical components of human milk oligosaccharides (HMOs). Rapid and efficient synthesis of these oligosaccharides is essential for investigating their functions and applications. In this study, a novel α2,3-sialyltransferase MsST was successfully identified through molecular docking and virtual screening. MsST was cascaded with CMP-sialic acid synthase to form a one-pot multienzyme system, which achieved a conversion rate exceeding 90% for various neutral core HMOs. Subsequently, the cascade enzymes were purified and immobilized in one step using nickel ferrite magnetic nanoparticles. The immobilized cascade enzymes retained 86% of their initial activity after 12 cycles and 70% at 37 °C after storage for 4 days, demonstrating high recyclability and storage stability. Therefore, seven sialylated HMOs, including three para α2,3-sialylated hexasaccharides, were synthesized from the neutral core HMOs in 91–96% recovery yields. This system provides an efficient platform for producing structurally defined α2,3-sialylated HMOs, facilitating the investigation of future functional food applications.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"7 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149374742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Transcriptomic and Metabolomic Analysis Elucidates Melatonin-Mediated Cold and Drought Tolerance during Grapevine Cane Overwintering 综合转录组学和代谢组学分析揭示了葡萄越冬过程中褪黑激素介导的耐寒性和抗旱性

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-10 DOI: 10.1021/acs.jafc.6c07869

Yuanyuan Wei,Guoqian Xu,Jin Dong,Kunyuan Gao,Nan Sun

{"title":"Integrated Transcriptomic and Metabolomic Analysis Elucidates Melatonin-Mediated Cold and Drought Tolerance during Grapevine Cane Overwintering","authors":"Yuanyuan Wei,Guoqian Xu,Jin Dong,Kunyuan Gao,Nan Sun","doi":"10.1021/acs.jafc.6c07869","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c07869","url":null,"abstract":"Cold-drought stress limits grapevine cane overwintering. Melatonin is known to enhance plant stress tolerance, but its regulatory mechanisms under natural overwintering remain unclear. We used the new grapevine line “140902” to investigate molecular responses to exogenous melatonin under combined cold-drought stress via integrated transcriptomic and metabolomic analyses. Melatonin increased endogenous hormones and antioxidant enzyme activities (e.g., SOD activity increased 23.6% under M3) and reduced MDA content by up to 31.9%, alleviating physiological damage. DEGs and DAMs were enriched in phenylpropanoid/flavonoid biosynthesis, hormone signaling, and starch/sucrose metabolism pathways. Melatonin upregulated JA/ABA signaling genes (GH3, JAZ, PYL), phenylpropanoid/flavonoid biosynthetic genes (C4H, 4CL, CAD, FLS), and carbon metabolism genes (β-glucosidase, cellulase), altering flavonoid (naringenin, eriodictyol) relative abundance. These coordinated changes may contribute to the melatonin-mediated alleviation of cold-drought injury in grapevines, offering insights into potential regulatory networks and a basis for nonburial cultivation in cold, arid northwestern China.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"64 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149374743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Basis for Altered Hydrolysis and Transglycosylation in a GH20 β-N-Acetylhexosaminidase Mutant: Insights from Molecular Dynamics Simulations GH20 β- n -乙酰己糖氨酸酶突变体水解和转糖基化改变的分子基础：来自分子动力学模拟的见解

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-10 DOI: 10.1021/acs.jafc.6c02292

Kunpeng Gao,Jing Gao,Hao Dong,Rui Chen,Zhuoning Cao,Na Guo,Chunhui Liu,Jianan Sun,Xiangzhao Mao

{"title":"Molecular Basis for Altered Hydrolysis and Transglycosylation in a GH20 β-N-Acetylhexosaminidase Mutant: Insights from Molecular Dynamics Simulations","authors":"Kunpeng Gao,Jing Gao,Hao Dong,Rui Chen,Zhuoning Cao,Na Guo,Chunhui Liu,Jianan Sun,Xiangzhao Mao","doi":"10.1021/acs.jafc.6c02292","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c02292","url":null,"abstract":"β-N-acetylhexosaminidase (NAHase) plays a critical role in the potential high-value transformation of chitin through its hydrolysis and transglycosylation activities. In this work, a novel GH20 NAHase (designated Fmhex) from Fulvivirga maritima was heterologously expressed and biochemically characterized, which showed the highest activity at 45 °C and pH 6.0. To regulate its catalytic properties, a saturated mutation library targeting five nonconserved residues was constructed. Mutants Fmhex(G417A) and Fmhex(I436S) demonstrated 2.3-fold hydrolysis activity and 1.9-fold transglycosylation activity, respectively. Classical molecular dynamics alone cannot fully explain the enhanced hydrolysis of Fmhex(G417A) since it only exhibited a shorter catalytic distance and increased sugar-ring distortion, without a complete near-attack geometry or binding advantage. Conversely, Fmhex(I436S) reshaped the internal pocket architecture, enabling more pocket contacts and hydrogen bonds, deeper burial, and more favorable energetics, providing a structural rationale for its enhanced transglycosylation. These findings provided mechanistic insights and testable hypotheses for engineering NAHases with tailored hydrolysis/transglycosylation balances.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"419-420 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149374744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xylo-Oligosaccharides Attenuate T-2 Toxin-Induced Pulmonary Fibrosis by Inhibiting Intestinal Overgrowth and Gut-to-Lung Translocation of Staphylococcus saprophyticus 低聚木糖通过抑制腐生葡萄球菌肠道过度生长和肠-肺易位减轻T-2毒素诱导的肺纤维化

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-10 DOI: 10.1021/acs.jafc.6c09811

Jianyu Qu,Ying Ma,Xin Sheng,Xin Jiang,Liu Xu,Jie Sheng,Yuanyuan Zhu,Yuanbin Wang,Changyi Pi,Yanbin Ding,Shuiping Liu,Chuan Zhou,Sisi Yan,Jing Wu,Lixin Wen,Xiangyan Liu,Yulong Yin,Eduardo R. Cobo,Ji Wang

{"title":"Xylo-Oligosaccharides Attenuate T-2 Toxin-Induced Pulmonary Fibrosis by Inhibiting Intestinal Overgrowth and Gut-to-Lung Translocation of Staphylococcus saprophyticus","authors":"Jianyu Qu,Ying Ma,Xin Sheng,Xin Jiang,Liu Xu,Jie Sheng,Yuanyuan Zhu,Yuanbin Wang,Changyi Pi,Yanbin Ding,Shuiping Liu,Chuan Zhou,Sisi Yan,Jing Wu,Lixin Wen,Xiangyan Liu,Yulong Yin,Eduardo R. Cobo,Ji Wang","doi":"10.1021/acs.jafc.6c09811","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c09811","url":null,"abstract":"T-2 toxin, a widespread mycotoxin frequently detected in food and feed, severely threatens public health and livestock breeding safety. Although reports indicate its potential to cause pulmonary injury, the underlying mechanisms remain unclear. Here, we demonstrated that T-2 toxin compromised intestinal barrier integrity and increased abundance of S. saprophyticus in both the ileum and the lungs, thereby triggering pulmonary inflammation and fibrosis in piglets and mice. Transplantation of fecal microbiota from T-2 toxin-exposed piglets into recipient piglets mimicked the phenomena. NOD2 gene knockdown in mice reversed S. saprophyticus-induced pulmonary damage. Besides, xylo-oligosaccharides (XOS) inhibited the proliferation of S. saprophyticus driven by T-2 toxin and its translocation from the ileum to the lungs, thereby suppressing the NOD2/NF-κB signaling pathway and ultimately alleviating pulmonary fibrosis. This study deciphers the pivotal role of S. saprophyticus in mycotoxin-induced pulmonary fibrosis, highlighting the gut microbiota as a therapeutic target for preventing and treating mycotoxin-induced lung injury.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"4 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149374746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Potential Probiotic Candidate Heyndrickxia ginsengihumi W1 is Associated with Increased Intestinal Stem Cell-Mediated Proliferation and Altered Barrier-Related Parameters: Potential Involvement of the Wnt/β-Catenin Pathway 潜在的益生菌候选者人参海因德里克氏菌W1与肠道干细胞介导的增殖增加和屏障相关参数的改变有关：Wnt/β-Catenin通路的潜在参与

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-10 DOI: 10.1021/acs.jafc.6c08447

Yujia Jing,Enting Qiao,Wenjun Wei,Yanxin Zhang,Yifan Wang,Li Song,Xuezhao Sun,Mengzhi Wang

{"title":"A Potential Probiotic Candidate Heyndrickxia ginsengihumi W1 is Associated with Increased Intestinal Stem Cell-Mediated Proliferation and Altered Barrier-Related Parameters: Potential Involvement of the Wnt/β-Catenin Pathway","authors":"Yujia Jing,Enting Qiao,Wenjun Wei,Yanxin Zhang,Yifan Wang,Li Song,Xuezhao Sun,Mengzhi Wang","doi":"10.1021/acs.jafc.6c08447","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c08447","url":null,"abstract":"Probiotic properties of the rumen-derived strain Heyndrickxia ginsengihumi W1 were evaluated by whole-genome analysis, in vitro assays, and a short-term mouse model. Genome analysis preliminarily indicated no acquired antimicrobial resistance genes or virulence factors. In mice, W1 administration showed no obvious adverse effects under the present short-term experimental conditions, and was associated with Lactobacillus enrichment and elevated lactate in the jejunum. W1 administration was also associated with changes in selected jejunal barrier-related parameters and with increased intestinal stem cell (ISC)-mediated epithelial proliferation, potentially through the Wnt/β-catenin pathway. Jejunal lactate correlated positively with crypt depth, PCNA, ISC markers, and Wnt/β-catenin components. In vitro, purified lactate and W1 cell-free supernatant, but not heat-killed W1, promoted sheep intestinal epithelial cell proliferation, and lactate upregulated Axin2, Ctnnb1, and PCNA, but this lactate–Wnt/β-catenin mechanism remains a hypothesis requiring further validation. These results indicate that W1 may be a promising rumen-derived probiotic candidate.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"23 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149374747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tau-Fluvalinate Inhibits the Molting Process of Lizards (Eremias argus) by Disrupting Thyroid Hormone Signaling tau -氟戊酸酯通过干扰甲状腺激素信号传导抑制蜥蜴的蜕皮过程

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-10 DOI: 10.1021/acs.jafc.6c09601

Leisen Zhang,Lin Rao,Qiong An,Jianzhong Li,Jing Chang

引用次数: 0

Discovery of Human Gut Bacteria Responsible for Biotransformation and Structural Diversification of Oat Steroidal Saponins 参与燕麦甾体皂苷生物转化和结构多样化的人类肠道细菌的发现

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-10 DOI: 10.1021/acs.jafc.6c14250

Juanjuan Hu,Salar Hafez-Ghoran,Fatemeh Taktaz,Shuwei Zhang,Pengpeng Zhou,Shengmin Sang

{"title":"Discovery of Human Gut Bacteria Responsible for Biotransformation and Structural Diversification of Oat Steroidal Saponins","authors":"Juanjuan Hu,Salar Hafez-Ghoran,Fatemeh Taktaz,Shuwei Zhang,Pengpeng Zhou,Shengmin Sang","doi":"10.1021/acs.jafc.6c14250","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c14250","url":null,"abstract":"Oat steroidal saponins undergo biotransformation by the gut microbiota, yet the bacteria involved and the structural diversity of the resulting metabolites remain incompletely characterized. Incubation of avenacoside B (AVE-B), a major oat steroidal saponin, with fecal microbiota from six human donors revealed marked interindividual variation in avenacoside E (AVE-E) production. Five bacterial species capable of AVE-B metabolism were isolated, with Bacteroides uniformis SS-F-0001 exhibiting the broadest metabolic profile. Quantitative PCR and carbohydrate-active enzyme profiling suggested that abundance alone did not explain conversion capacity. Comparative 1D and 2D NMR analysis of isolated AVE-E and its isomer revealed diagnostic C-22 and C-25 chemical-shift differences and H2-26 → C-22 HMBC correlations, supporting an isonuatigenin-type isomer with a six-membered F ring. These findings identify bacterial contributors to oat saponin metabolism and reveal structural diversity involving both deglycosylation and aglycone ring rearrangement, providing a basis for investigating the mechanisms and biological consequences of microbial saponin metabolism.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"23 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149374788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0