Journal of Agricultural and Food Chemistry最新文献

{"title":"Expression of Recombinant Human α-Glucosidase in HEK293 Cells","authors":"So Nishimoto, Anaïs Debarbat, Yuki Ikeda, Emi Arikawa, Yuki Odagaki, Haruna Yano, Ying Qiao, Masaaki Ito, Toshiyuki Kimura, Teisuke Takita, Kiyoshi Yasukawa","doi":"10.1021/acs.jafc.4c06902","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06902","url":null,"abstract":"In mammals, intestinal α-glucosidase exists as a maltase–glucoamylase complex (MGAM) and a sucrase–isomaltase complex (SI). In this study, we transiently expressed human MGAM and SI in human embryonic kidney 293 (HEK293) cells. At pH 6.0 and 37 °C, the MGAM-expressing HEK293 cells extract (MGE) exhibited maltase, glucoamylase, and isomaltase activities but not sucrase activity, whereas the SI-expressing HEK293 cells extract (SIE) exhibited sucrase, isomaltase, and maltase activities but not glucoamylase activity. The apparent <i>K</i>_m value of the MGE for maltose hydrolysis was 14–26% of that of the SIE for maltose, sucrose, and isomaltose hydrolysis. The respective apparent <i>V</i>_max values of the MGE and SIE for sucrose and isomaltose hydrolysis were 0% and 6% and 10% and 42% of those for maltose hydrolysis. These results indicated that the maltase activities of MGAM and SI were higher than those of sucrase and isomaltase.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"83 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat Tolerance-Associated circRNA3685 Regulates Apoptosis and Autophagy in Bovine Mammary Epithelial Cells via Sponging bta-miR-138","authors":"Congcong Zhang, Lirong Hu, Shuhui Wang, Gong Chen, Luiz F. Brito, Bin Li, Qing Xu, Yachun Wang","doi":"10.1021/acs.jafc.4c07967","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07967","url":null,"abstract":"Heat stress negatively affects dairy cow production, and health, leading to significant losses. Identifying mechanisms associated with heat tolerance is crucial for developing breeding strategies. Circular RNAs (circRNAs), a type of noncoding RNA, regulate cell functions like autophagy, apoptosis and proliferation. In this study, dairy cows were classified into heat stress tolerant (HST, <i>n</i> = 15) and heat stress sensitive (HSS, <i>n</i> = 15) groups based on respiratory and drooling score during the heat stress. A significant difference in milk production decline was observed, with the HST group showing less decline, indicating better heat tolerance. Blood transcriptomics analysis identified 166 differentially expressed circRNAs with circRNA3685 being highlighted as a key candidate linked to heat tolerance. Overexpression of circRNA3685 in bovine mammary (MAC-T) cells inhibited autophagy and apoptosis. The circRNA3685 was found to interact with bta-miR-138, targeting HIF1A. These findings provide insights into circRNAs’ role in heat stress adaptation in dairy cows.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"112 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Feature of Salty/Saltiness-Enhancing Peptides Derived from Coprinus comatus and Their Stability during Subsequent Thermal Treatment and Maillard Reaction","authors":"Mingzhou Song, Tong Zhou, Qiuhong Liao, Foxin Zhang, Shahzad Hussain, Khizar Hayat, Xiaoming Zhang, Chi-Tang Ho","doi":"10.1021/acs.jafc.4c10426","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10426","url":null,"abstract":"Through a quantitative analysis of saltiness perception, favorable enzymatic hydrolysis parameters were confirmed for the preparation of saltiness-enhancing peptide mixtures from <i>Coprinus comatus</i>. The enzymatic hydrolysate was fractionated into four fractions (F1–F4) by gel chromatography, with F3 exhibiting the strongest saltiness-enhancing effect (22% increase). LC-MS/MS analysis of F3 identified 36 peptides, and their secondary structures and interactions with the TMC4 receptor were examined through circular dichroism spectroscopy and molecular docking. Molecular docking analysis revealed Asn588, Ser165, Asp5, and Arg168 as key amino acid residues, with the peptide GDNVGF showing the lowest binding energy. Synthetic GDNVGF (0.01%) in 70 mmol/L NaCl enhanced saltiness by 17%. When 0.7% GDNVGF was added to the aqueous solution, its saltiness was equivalent to that of 36.89 mmol/L NaCl, which suggested that GDNVGF functions both as a saltiness-enhancing peptide and a salty peptide. The taste changes of peptides during thermal reactions were further investigated. The thermal stability of <i><i>Coprinus comatus</i></i> peptides was good, but their saltiness-enhancing effect slightly reduced due to thermal degradation. The Maillard reaction further diminished this effect, though the umami level remained satisfactory, offering new insights into using <i><i>Coprinus comatus</i></i> peptides as low-sodium salt substitutes.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"17 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conjugate Position of Glucuronide and Sulfate in Piceatannol Derivatives Affects the Stability and Hydrolytic Resistance of the Conjugate in Biological Matrices","authors":"Miyu Nishikawa, Mai Nakayama, Keisuke Fukaya, Daisuke Urabe, Shinichi Ikushiro","doi":"10.1021/acs.jafc.4c08072","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08072","url":null,"abstract":"Piceatannol, a stilbene compound, undergoes a comprehensive phase II metabolism mediated by UDP-glucuronosyltransferases (UGTs) and sulfotransferases (SULTs) in humans. Despite their well-documented beneficial effects on health, their detailed pharmacokinetic fate, including the metabolite structure and properties, is poorly understood. Thus, we determined the structure of seven glucuronides and six sulfates transformed from piceatannol and its methylated derivatives in recombinant yeast cells expressing UGTs or SULTs. We evaluated their properties in human and rat plasma samples. The conjugate that was substituted at the 3′- or 4′-catecholic hydroxy moiety exhibited increased stability. The sulfatase-mediated hydrolysis assay results in incomplete digestion or compound degradation of certain sulfates, suggesting a potential risk of underestimation by using indirect quantification methods. These findings emphasize the importance of an authentic standard for accurate pharmacokinetic studies of phase II metabolites that will be useful for understanding the mechanisms underlying the functional contribution of piceatannol in the body.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volatile Byproducts of Carotenoid Degradation as Biomarkers of Maize Infestation by the Maize Weevil (Sitophilus zeamais) (Motsch.)","authors":"Geoffrey T. Broadhead, Hui Liu, Gretchen L. Sumrall, Anna K. Block, Charles T. Hunter, John J. Beck","doi":"10.1021/acs.jafc.4c09665","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09665","url":null,"abstract":"Maize (<i>Zea mays</i>) is a major global food crop and a source of industrial raw materials. Effective postharvest storage is important for national food security programs, international trade, and global agriculture economics. The maize weevil (<i>Sitophilus zeamais</i>) is a primary postharvest insect pest that infests maize during storage and leads to significant losses. Using multivariate discriminant analysis of volatile profiles collected from intact and infested maize, we identified two volatile apocarotenoids, 6-methyl-5-hepten-2-one and 6-methyl-5-hepten-2-ol, as indicators of maize weevil infestation in stored maize. Emission of these biomarker compounds rapidly signaled maize weevil infestation and showed a significant correlation with oviposition damage to the stored kernels. The pattern of elevated biomarker emission after weevil exposure was consistent across all seven maize lines examined. These volatile biomarkers can be used for early detection and removal of infested maize and can aid in the control of this pest.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"269 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing Succinic Acid Biomanufacturing Using the Nonconventional Yeast Yarrowia lipolytica","authors":"Tao Sun, Mei-Li Sun, Lu Lin, Jian Gao, Kaifeng Wang, Xiao-Jun Ji","doi":"10.1021/acs.jafc.4c09990","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09990","url":null,"abstract":"Succinic acid is an essential bulk chemical with wide-ranging applications in materials, food, and pharmaceuticals. With the advancement of biotechnology, there has been a surge in focus on low-carbon sustainable microbial synthesis methods for producing biobased succinic acid. Due to its high intrinsic acid tolerance, <i>Yarrowia lipolytica</i> has gained recognition as a competitive chassis for the industrial manufacture of succinic acid. This review summarizes the research progress on succinic acid biomanufacturing using <i>Y. lipolytica</i>. First, it introduces the major metabolic routes for succinic acid biosynthesis and the pertinent engineering approaches for building efficient cell factories. Subsequently, we offer a review of methods employed for succinic acid synthesis by <i>Y. lipolytica</i> utilizing alternative substrates as well as the relevant optimization strategies for the fermentation process. Finally, future research directions for improving succinic acid biomanufacturing in <i>Y. lipolytica</i> are delineated in light of the recent progress, obstacles, and trends in this area.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design, Synthesis, and Herbicidal Activity Study of Novel Pyrazole-Carboxamides as Potential Transketolase Inhibitors","authors":"Chengkun Li, Junmin Wang, Haijiao Dong, Dongchen Yang, Peng Li, Shuang Cao, Chao Li, Zexiu An, Jinlin Zhang, Yan-En Wang","doi":"10.1021/acs.jafc.4c08397","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08397","url":null,"abstract":"Transketolase (TKL; EC 2.2.1.1) has been identified as a potential new herbicide target. In order to discover highly herbicidal active compounds targeting TKL and improve their structural diversity for lead compounds, a series of pyrazole-carboxamides <b>7a</b>–<b>7v</b> were designed and synthesized through structural optimization for pyrazole-containing phenoxy amide compound <b>4u</b>. Among the synthesized compounds, compound <b>7r</b> possessed excellent herbicidal efficacy against <i>Digitaria sanguinalis</i> (<i>Ds</i>) and <i>Amaranthus retroflexus</i> (<i>Ar</i>) by the small cup method (the inhibition about 95%, 100 mg/L) and the foliar spray method (the inhibition over 90%, 150 g ai/ha) in a greenhouse, which were superior to that of the positive control nicosulfuron. More significantly, compound <b>7r</b> displayed good crop selectivity toward both maize and wheat even at 375 g of ai/ha. The studies on mode of action (MOA) of high herbicidal active compounds, including the enzyme inhibition activity, fluorescent quenching experiments, and molecular docking analysis between <i>Setaria viridis</i> (<i>Sv</i>)TKL and ligand, suggested that compound <b>7r</b> acts as a typical TKL inhibitor, and the benzothiazole ring is an important motif for <i>Sv</i>TKL inhibition activity. Above all, compound <b>7r</b> could be a potential candidate for the development of herbicides with new MOA for weed control in maize and wheat field.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"82 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “CF2–II Alternative Splicing Isoform Regulates the Expression of Xenobiotic Tolerance-Related Cytochrome P450 CYP6CY22 in Aphis gossypii Glover”","authors":"Yaping Ding, Jianyi Li, Kunpeng Yan, Long Jin, Chengcheng Fan, Rui Bi, Haoran Kong, Yiou Pan, Qingli Shang","doi":"10.1021/acs.jafc.4c11996","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11996","url":null,"abstract":"A corrected version of Figure 9 appears below. An incorrect image appeared in the negative control in the original version. No bacteria could grow in medium lacking Leu with ABA (600 ng/mL) in the negative control. This correction does not change the scientific conclusions of the article. Figure 9. Structure of the <i>CYP6CY22</i> promoter region. (A) Structure of the 5′-flanking region of <i>CYP6CY22</i>. The TSS in the figure represents the transcription start site. The transcription starting point number is +1, with the upstream sequence starting with “–” and the downstream sequence starting with “+”. The predicted <i>CF</i>₂<i>–II-AS</i> protein binding sites and mutant sequence are indicated. (B) Investigation of the interaction between <i>CYP6CY22.p</i> and <i>CF</i>₂<i>–II-AS</i> by the Y1H assay. Yeast transformants containing the <i>CYP6CY22.p</i> vector and TF plasmid were selected on SD/-Leu media supplemented with ABA. Positive control: yeast strain transformed with the pGADT7-<i>p53</i> and pAbAi-<i>p53</i> plasmids; negative control: yeast strain transformed with pAbAi-<i>CYP6CY22.p</i>. This article has not yet been cited by other publications.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"77 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome Mining Reveals Biosynthesis of the Antifungal Lipopeptaibols, Texenomycins, through a Hybrid PKS-NRPS System, in the Fungus Mariannaea elegans","authors":"Yang Jiao, Jian Ling, Raja Asad Ali Khan, Ning Luo, Zixin Li, Zeyu Li, Yuhong Yang, Jianlong Zhao, Zhenchuan Mao, Gerald F. Bills, Bingyan Xie, Yan Li","doi":"10.1021/acs.jafc.4c08847","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08847","url":null,"abstract":"Texenomycins are a family of linear lipopeptaibols with a long polyketide side chain at the N-terminus and 21 amino acid residues at the <i>C</i>-terminus, presenting demonstrated potential as antibiotics against plant fungal pathogens. In this study, texenomycins were identified and isolated from the fungus <i>Mariannaea elegans</i> strain TTI-0396 and showed effective antifungal properties against two plant pathogens <i>Colletotrichum lagenarium</i> and <i>Botrytis cinerea</i>. Through analysis of the whole-genome data of <i>M. elegans</i> strain TTI-0396, we discovered a hybrid PKS-NRPS system with the polyketide synthase (PKS: TexQ), thioesterase (TexO), acyl-CoA ligase (TexI), and three nonribosomal peptide synthetases (NRPSs: TexG, TexJ, TexV) in the <i>tex</i> gene cluster that were proposed to be responsible for the biosynthesis of texenomycins and another related lipopeptaibol, lipohexin. The functions of six key genes (<i>texQ</i>, <i>texO</i>, <i>texI</i>, <i>texG, texJ</i>, and <i>texV</i>) in the hybrid PKS-NRPS system were verified by gene deletion experiments, and five genes (<i>texQ</i>, <i>texO</i>, <i>texI</i>, <i>texG</i>, and <i>texV</i>) were confirmed to be responsible for the biosynthesis of texenomycins, while four genes (<i>texQ</i>, <i>texO</i>, <i>texI</i>, and <i>texJ</i>) were involved in the biosynthesis of lipohexin. Furthermore, the function of one transcription factor gene (<i>texR</i>), which enhanced the production of texenomycins by regulating the key genes in the <i>tex</i> gene cluster, was also demonstrated through gene deletion and overexpression experiments. Finally, a hypothetical scheme for texenomycins and lipohexin biosynthesis assembly is proposed. The elucidation of this intricate hybrid PKS-NRPS system has significantly deepened our comprehension of the mechanisms underlying the generation and chemical diversity of fungal lipopeptaibol natural products, offering a promising avenue for future research and potential applications in fungicidal disease control in agriculture.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"11 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemoproteomic Profiling Reveals Chlorogenic Acid as a Covalent Inhibitor of Arabidopsis Dehydroascorbate Reductase 1","authors":"Jingyuan Xu, Lijun Chen, Shanshan Wang, Wen Zhang, Jianjia Liang, Lu Ran, Zhangshuang Deng, Yiqing Zhou","doi":"10.1021/acs.jafc.4c07955","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07955","url":null,"abstract":"Chlorogenic acid (CA) is an abundant plant secondary metabolite with promising allelopathic effects on weed growth. However, the molecular targets and mechanism of action of CA in plants remain elusive. Here, we report the employment of a clickable photoaffinity probe in identifying the protein targets of CA in <i>Arabidopsis</i> seedling proteomes. CA specifically binds <i>Arabidopsis</i> dehydroascorbate reductase 1 (<i>At</i>DHAR1), an enzyme responsible for ascorbate regeneration in plants, by covalent alkylating Cys20 within the catalytic center, thereby inhibiting its activity. In vivo application of CA reduced the pool size and redox state of ascorbate, leading to H₂O₂ accumulation in <i>Arabidopsis</i> seedlings. In agreement with these results, CA significantly induced the upregulation of antioxidant enzymes and downregulation of proteins involved in water transport and photosynthesis, as evidenced by quantitative proteomics. Taken together, this study revealed DHAR1 as a functional target underlying CA’s allelopathic activity in plants, which opens new opportunities for the development of novel herbicides from naturally existing resources.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"70 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}