Journal of Agricultural and Food Chemistry最新文献

Bisdemethoxycurcumin and Curcumin Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.jafc.4c11101

Kai-Yu Hsu, Anju Majeed, Chi-Tang Ho, Min-Hsiung Pan

{"title":"Bisdemethoxycurcumin and Curcumin Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice","authors":"Kai-Yu Hsu, Anju Majeed, Chi-Tang Ho, Min-Hsiung Pan","doi":"10.1021/acs.jafc.4c11101","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11101","url":null,"abstract":"Curcuminoids, found in turmeric (Curcuma longa L.), include curcumin (CUR), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC). Although CUR and DMC are well-studied, the anti-inflammatory effects of BDMC remain less explored. Recent studies highlight BDMC’s stronger NF-κB inhibition compared to CUR and DMC in cell models, along with its ability to target pathways associated with inflammatory bowel disease (IBD) in DSS-induced colitis mice, reflected by lower disease activity scores and reduced inflammation. This study assessed CUR and BDMC in a DSS-induced colitis mouse model. Dietary administration of CUR or BDMC strengthened tight junction (TJ) proteins, reduced inflammatory cytokine secretion, and attenuated intestinal inflammatory protein expression, thereby alleviating DSS-induced IBD in mice. Furthermore, gut microbiota and short-chain fatty acid analyses revealed that CUR and BDMC effectively regulated gut microbial imbalance and promoted the relative abundance of butyrate-producing bacteria. Furthermore, CUR showed low absorption and was primarily excreted in feces, while BDMC had higher absorption levels. In conclusion, while both BDMC and CUR have potential as adjunct therapies for IBD, BDMC at a concentration of 0.1% showed strong anti-inflammatory effects and enhanced TJ proteins, suggesting that BDMC, even at lower concentrations than CUR, holds promising therapeutic potential and prospects.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"117 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome Reveals the Promoting Effect of Beta-Sitosterol on the Differentiation of Bovine Preadipocytes

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.jafc.4c10452

Lei Jiang, Xiaolei Sun, Yuan Wan, Qihua Qin, Min Xu, Jianqiang Ma, Linsen Zan, Hongbao Wang

{"title":"Transcriptome Reveals the Promoting Effect of Beta-Sitosterol on the Differentiation of Bovine Preadipocytes","authors":"Lei Jiang, Xiaolei Sun, Yuan Wan, Qihua Qin, Min Xu, Jianqiang Ma, Linsen Zan, Hongbao Wang","doi":"10.1021/acs.jafc.4c10452","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10452","url":null,"abstract":"Natural small molecule compounds play crucial roles in regulating fat deposition. Beta-sitosterol exhibits multiple biological activities such as cholesterol reduction and anticancer effects. However, its regulatory mechanism in the differentiation of bovine preadipocytes remains unclear. We identified potential associations of Beta-sitosterol with biological processes such as cholesterol regulation and lipid metabolism through the prediction of its targets. We utilized techniques such as Oil Red O staining, Western blotting, RNA-seq, and others to elucidate the promoting effect of Beta-sitosterol on the differentiation of bovine preadipocytes. Furthermore, reducing the expression of the most downregulated gene among differential expressed genes (DEGs), MGP, promotes the differentiation of bovine preadipocytes. After interfering with MGP, RNA-seq analysis on the sixth day of differentiation revealed that DEGs were most significantly enriched in the PPAR signaling pathway. In this pathway, the expression levels of genes related to adipocyte differentiation, including CD36, RXRα, RXRγ, FABP4, PLIN1, ADIPO, and CAP, were significantly upregulated (P < 0.01). Western blot and ELISA analysis on genes related to the PPAR signaling pathway showed that interfering with MGP increased the expression of proteins such as RXRα, indicating the possible activation of the PPAR signaling pathway. In summary, Beta-sitosterol may promote the differentiation of bovine preadipocytes by reducing the expression of MGP, thereby activating the PPAR signaling pathway.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"59 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suckling Rat Pup Model: Do Caprine Milk Lactoferrin and Immunoglobulins Have Different Digestion and Absorption Properties from That of Human and Bovine Species?

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.jafc.4c10539

Lina Zhang, Ying Ma, Kasper Hettinga, Peng Zhou

{"title":"Suckling Rat Pup Model: Do Caprine Milk Lactoferrin and Immunoglobulins Have Different Digestion and Absorption Properties from That of Human and Bovine Species?","authors":"Lina Zhang, Ying Ma, Kasper Hettinga, Peng Zhou","doi":"10.1021/acs.jafc.4c10539","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10539","url":null,"abstract":"This study aimed to investigate the digestion and absorption properties of caprine milk serum proteins in comparison to human and bovine species by using rat pups to mimic preterm infants. The results indicate that caprine lactoferrin (LTF) had a shorter retention time in the intestine and released a greater number of fragments, resembling human milk LTF more closely. In contrast, caprine immunoglobulins (Igs) were similar to bovine Igs and both exhibited a longer retention time in the intestine. For absorption, caprine Igs could be absorbed intact, which was similar to human and bovine Igs, whereas caprine LTF fragments were found in jejunum but not in plasma of rat pups. This is similar to bovine LTF but differed from human LTF as human LTF could be absorbed intact in plasma of rat pups at 20 min. In addition, the absorption rate of peptides and amino acids from caprine milk serum was similar to that of human milk serum, which was higher than that from bovine milk serum. This study aimed to enhance our understanding of the differences in bioavailability of LTF and Igs derived from caprine, human milk, and bovine milk, thereby offering guidance for selecting protein sources for premature infants.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"64 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quinoa Ethanol Extract Ameliorates Cognitive Impairments Induced by Hypoxia in Mice: Insights into Antioxidant Defense and Gut Microbiome Modulation

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.jafc.4c07530

Yongli Lan, Yujie Song, Wengang Zhang, Shiyang Zhao, Xinze Wang, Lei Wang, Yutang Wang, Xijuan Yang, Hao Wu, Xuebo Liu

{"title":"Quinoa Ethanol Extract Ameliorates Cognitive Impairments Induced by Hypoxia in Mice: Insights into Antioxidant Defense and Gut Microbiome Modulation","authors":"Yongli Lan, Yujie Song, Wengang Zhang, Shiyang Zhao, Xinze Wang, Lei Wang, Yutang Wang, Xijuan Yang, Hao Wu, Xuebo Liu","doi":"10.1021/acs.jafc.4c07530","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07530","url":null,"abstract":"Quinoa, rich in pharmacologically active ingredients, possesses the potential benefit in preventing cognitive impairments induced by hypoxia. In this study, the efficacy of quinoa ethanol extracts (QEE) consumption (200 and 500 mg/kg/d, respectively) against hypobaric hypoxia (HH)-induced cognitive deficits in mice was investigated. QEE significantly ameliorated hypoxic stress induced by HH, as evidenced by improvements in baseline indices and reductions in hypoxia-inducible factor 1α levels. Furthermore, QEE enhanced antioxidant defense mechanisms, alleviated neuroinflammation in brain regions associated with memory, and improved HH-induced cognitive impairments by modulating the cyclic adenosine monophosphate response element-binding protein/brain-derived neurotrophic factor signaling pathway. Higher doses generally yielded more effective outcomes than lower doses. QEE also significantly reshaped the gut microbiome structure of HH mice, inhibited gut barrier damage, and reduced lipopolysaccharide migration, thereby increasing short-chain fatty acids (SCFAs) levels. Our findings suggest that QEE may be a promising strategy for preventing hypoxia-induced cognitive impairments by maintaining gut microbiome stability and increasing SCFAs levels.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"25 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of the Discontinuous Structure in Heat-Induced Natural Egg Yolk Mediated by Accumulation of Yolk Sphere Microgels: Morphology, 4D-DIA Proteomics, and Physicochemical Properties

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-27 DOI: 10.1021/acs.jafc.4c11324

Keshan Wang, Xianmin Qin, Chan Li, Jiao Wu, Xiaoli Liu, Jiaxuan Ma, Ying Zhou, Qiujin Zhu, Yuanyuan Liu, Yongguo Jin

{"title":"Mechanism of the Discontinuous Structure in Heat-Induced Natural Egg Yolk Mediated by Accumulation of Yolk Sphere Microgels: Morphology, 4D-DIA Proteomics, and Physicochemical Properties","authors":"Keshan Wang, Xianmin Qin, Chan Li, Jiao Wu, Xiaoli Liu, Jiaxuan Ma, Ying Zhou, Qiujin Zhu, Yuanyuan Liu, Yongguo Jin","doi":"10.1021/acs.jafc.4c11324","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11324","url":null,"abstract":"The heat-induced natural egg yolk is a discontinuous object formed by the accumulation of yolk spheres. However, the reason why yolk spheres form individual microgels rather than continuous gels has not been elucidated. This study investigated the different gelation behaviors in the yolk sphere exterior (EYSE) and the yolk sphere interior (EYSI) by using 4D-DIA proteomics, electron microscopy, and multispectral techniques. Results demonstrated that vitellogenin-1, -2, and -3 (EYSI/EYSE fold change: 3.36, 3.53, and 2.42, respectively) were key proteins corresponding to continuous gel structure formation of EYSI. However, the high levels of apolipoprotein A-I (FC: 0.18) and heat shock protein found in EYSE with a special hydrophobic domain for lipid binding impeded the continuous gel formation. Thus, the EYSE formed some small-volume aggregates without continuous gel, which separated individually the microgel of yolk spheres. This study will provide theoretical guidance for the quality regulation of egg yolk products.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"63 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Core–Satellite Gold Nanoparticle@Silver Nanocluster Nanohybrids for Milk Allergen β-Lactoglobulin Detection Using the Electrochemical Aptasensor

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-27 DOI: 10.1021/acs.jafc.4c08948

Tingting Bai, Yanjia Liu, Zhien Liu, Yue Teng, Pin Liu, Liusi Peng, Daohong Wu

{"title":"Core–Satellite Gold Nanoparticle@Silver Nanocluster Nanohybrids for Milk Allergen β-Lactoglobulin Detection Using the Electrochemical Aptasensor","authors":"Tingting Bai, Yanjia Liu, Zhien Liu, Yue Teng, Pin Liu, Liusi Peng, Daohong Wu","doi":"10.1021/acs.jafc.4c08948","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08948","url":null,"abstract":"Detecting β-lactoglobulin (β-Lg) with high sensitivity and selectivity is an urgent requirement due to nearly 80% of milk anaphylaxis, such as respiratory tract, skin urticaria, and gastrointestinal disorders, being caused by β-Lg. An ultrasensitive β-Lg electrochemical aptasensor utilizing core–satellite gold nanoparticle@silver nanocluster (AuNPs@AgNCs) nanohybrids as electrocatalysts was developed. First, β-Lg aptamer was anchored on gold electrodes and AuNPs to obtain high selectivity. Aptamer-cDNA-AuNPs conjugation was attached to the functionalized electrode with β-Lg as a “bridge” through the target–ligand interaction. Second, DNA-templated AgNCs were introduced via the hybridization of DNA templates oligonucleotide with cDNA anchored on AuNPs. The formed AuNPs@AgNCs nanohybrids showed enhanced catalytic performance toward the silver deposition reaction. This strategy is demonstrated by determining the oxidation current of produced silver nanoparticles (AgNPs) surrounding AuNPs by β-Lg. A detection limit of 0.87 fg/mL and a linear range of 0.001–1000 pg/mL were obtained. Finally, β-Lg content in food products was analyzed successfully, and RSD of 2.44–8.33% was obtained. The recovery of 87.54–113.70% and RSD of 0.95–9.29% was obtained for standard addition experiments. This proposed aptasensor exhibits excellent sensitivity, selectivity, reproducibility, and stability and has good practical application capability for complex food matrices.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"32 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of 14 Lactic Acid Bacteria for Fermentative Isomalto/Malto-Polysaccharide Synthesis

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-27 DOI: 10.1021/acs.jafc.4c09286

Nele Brand, Daniel Wefers

引用次数: 0

Nanoization of Technical Pesticides: Facile and Smart Pesticide Nanocapsules Directly Encapsulated through “On Site” Metal–Polyphenol Coordination Assembly for Improved Efficacy and Biosafety

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-27 DOI: 10.1021/acs.jafc.4c10948

Yuxia Chen, Hang Li, Yuhui Peng, Tongtong Li, Xiaona Li, Chen Wang, Ruixi Xiao, Jiangtao Dong, Xuezhong Du

{"title":"Nanoization of Technical Pesticides: Facile and Smart Pesticide Nanocapsules Directly Encapsulated through “On Site” Metal–Polyphenol Coordination Assembly for Improved Efficacy and Biosafety","authors":"Yuxia Chen, Hang Li, Yuhui Peng, Tongtong Li, Xiaona Li, Chen Wang, Ruixi Xiao, Jiangtao Dong, Xuezhong Du","doi":"10.1021/acs.jafc.4c10948","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10948","url":null,"abstract":"Facile pesticide nanocapsules were successfully prepared by directly encapsulating the antisolvent precipitation of pesticides through instantaneous “on site” coordination assembly of tannic acid and Fe3+, avoiding tedious preparation, time consumption, and large amounts of organic solvents. The pesticide nanocapsules showed excellent resistance to ultraviolet photolysis and rainwater washing owing to the nanocapsule walls. The smart pesticide nanocapsules exhibited the controlled release of pesticides under multidimensional stimuli, such as acidic/alkaline pH, glutathione, H2O2, phytic acid, laccase, tannase, and sunlight, which were related to the physiological and natural environments of crops, pests, and pathogens. The tebuconazole nanocapsules not only enhanced the fungicidal activity against Fusarium graminearum and effective control efficacy in wheat powdery mildew through foliar spray and seed coating, but also improved the biosafety of target plant growth and nontarget organisms. The facile, smart, efficient, safe, and green pesticide nanocapsules using the universal strategy have broad application prospects in ecoagriculture.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"35 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T-2 Toxin Nephrotoxicity: Toxic Effects, Mechanisms, Mitigations, and Future Perspectives

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-27 DOI: 10.1021/acs.jafc.4c10015

Youshuang Wang, Pengli Liu, Jiayan Fan, Shuo Li, Xinyu Zhang, Yanan Li, Xuebing Wang, Cong Zhang, Xu Yang

{"title":"T-2 Toxin Nephrotoxicity: Toxic Effects, Mechanisms, Mitigations, and Future Perspectives","authors":"Youshuang Wang, Pengli Liu, Jiayan Fan, Shuo Li, Xinyu Zhang, Yanan Li, Xuebing Wang, Cong Zhang, Xu Yang","doi":"10.1021/acs.jafc.4c10015","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10015","url":null,"abstract":"T-2 toxin is a highly toxic fungal toxin that threatens humans and animals’ health. As a major detoxifying and metabolic organ, the kidney is also a target of T-2 toxin. This article reviews T-2 toxin nephrotoxicity research progress, covering renal structure and function damage, nephrotoxicity mechanisms, and detoxification methods to future research directions. T-2 toxin damages kidney structure, causing renal dysfunction. The nephrotoxicity mechanism of T-2 toxin involves multiple factors including oxidative stress, endoplasmic reticulum stress, mitophagy, inflammatory responses, and apoptosis, which are intertwined and interdependent. Current detoxification strategies mainly involve reducing T-2 toxin in feedstuff and using antioxidant substances, but both have limits. Future research should focus on renal cells sensitivity to T-2 toxin, exploring key molecules in T-2 toxin’s nephrotoxicity, renal injury’s impact on other organs, and better detoxification methods. This review aims to guide future research and underpin T-2 toxin-induced nephrotoxicity prevention and treatment.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"22 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Internal Environmental Changes of Muscle Cells and Apoptotic Phase of Mitochondria in Dry-Cured Loin Using Electrical Stimulation: Promoting the Precise Regulation of Loin Ham Quality

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-27 DOI: 10.1021/acs.jafc.4c09673

Li Chen, Lisha Lan, Shenghui Bi, Mixin Zhou, Jing Wan, Ying Zhou, Yuanyuan Liu, Qiujin Zhu

{"title":"Exploring the Internal Environmental Changes of Muscle Cells and Apoptotic Phase of Mitochondria in Dry-Cured Loin Using Electrical Stimulation: Promoting the Precise Regulation of Loin Ham Quality","authors":"Li Chen, Lisha Lan, Shenghui Bi, Mixin Zhou, Jing Wan, Ying Zhou, Yuanyuan Liu, Qiujin Zhu","doi":"10.1021/acs.jafc.4c09673","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09673","url":null,"abstract":"Traditional dry-curing methods have a long cycle time and low efficiency, resulting in the inconsistent quality of dry-cured ham. By applying electrical stimulation (ES) technology in the dry-curing process, it was found that ES affected mitochondrial apoptosis by modulating the intracellular environment of muscle cells, which, in turn, enhanced the quality of dry-cured pork loin. Specifically, ES accelerated glycogen and ATP depletion, which led to a rapid decline in pH. Meanwhile, compared to the control group (CK), the activities of Na+-K+-ATPase and Ca2+-ATPase in the ES group (ES) increased by 160% and 124%, respectively, leading to the generation of H+ gradient and Ca2+ overload in mitochondria, which in turn triggered mitochondrial apoptosis and increased the apoptosis rate by 259%. In addition, Western blot analysis showed that ES promoted the Desmin and Troponin-T degradation levels. This study highlights the advantages of ES in dry-cured ham processing, which is expected to be a precise regulation technology.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"40 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0