Journal of Agricultural and Food Chemistry最新文献

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In Vino Analytica Scientia 2024: Symposium History, Comment on the 13th Installment and Welcome to the Special Issue
IF 5.7 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-02-12 DOI: 10.1021/acs.jafc.4c1280810.1021/acs.jafc.4c12808
Caroline Bamforth, Andrew Clark*, Susan E. Ebeler and Chantal Maury, 
{"title":"In Vino Analytica Scientia 2024: Symposium History, Comment on the 13th Installment and Welcome to the Special Issue","authors":"Caroline Bamforth,&nbsp;Andrew Clark*,&nbsp;Susan E. Ebeler and Chantal Maury,&nbsp;","doi":"10.1021/acs.jafc.4c1280810.1021/acs.jafc.4c12808","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c12808https://doi.org/10.1021/acs.jafc.4c12808","url":null,"abstract":"<p >Since 1997, the In Vino Analytica Scientia symposia (IVAS) have provided an international platform for researchers to present novel analytical science relevant to grapes, wines, and spirits. Within this article the symposium history is outlined, paying homage to those who have contributed so much to the ongoing success of IVAS. Details for the 13th edition of IVAS, held at the University of California Davis campus on 9–12th July, are provided along with a welcome to the IVAS/JAFC special issue.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"73 6","pages":"3326–3329 3326–3329"},"PeriodicalIF":5.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanoporous 3D Polyurethane for Toosendanin Adsorption, Encapsulation, and High-Efficient Utilization
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-02-11 DOI: 10.1021/acs.jafc.4c09493
Wen-kui Li, Song Wang, Yong-hong Wang, Yu-zhen Wu, Jia Li, Tian-hua Chai, Kang Wang, GuangYou Chen, Zhiqing Ma
{"title":"Nanoporous 3D Polyurethane for Toosendanin Adsorption, Encapsulation, and High-Efficient Utilization","authors":"Wen-kui Li, Song Wang, Yong-hong Wang, Yu-zhen Wu, Jia Li, Tian-hua Chai, Kang Wang, GuangYou Chen, Zhiqing Ma","doi":"10.1021/acs.jafc.4c09493","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09493","url":null,"abstract":"Nanoporous 3D-polyurethane (3D-PU) was prepared based on nano-CaCO<sub>3</sub> templated controllably confined polymerization assembly and weak acid etching strategies. Nanopores with diameters ranging from 48 to 72 nm were distributed on 3D-PU, facilitating its high BET surface area of 468.0 m<sup>2</sup>/g. The 3D-PU exhibited enhanced adsorption selectivity to multi-H-bond donors and acceptors, multirings contained compounds based on pore filling, hydrogen-bonding, and π–π interactions; therefore, the novel 3D-PU had promising adsorption ability to toosendanin (TSN) with a maximum theoretical adsorption capacity of 361.6 mg/g. Adsorption isotherm, kinetic, and thermodynamic investigations revealed that the adsorption was heterogeneous and was supported by multiple adsorption sites, controlled by a chemical adsorption mechanism, endothermic, spontaneous, and with increased entropy. Based on the optimized adsorption, the loading capacity (LC) of 3D-PU toward TSN attained 23.4%. After encapsulation, the effective period of TSN was extended to 11 days, the photolysis half-life of TSN was increased 3.2 times, and the LC<sub>50</sub> for <i>Aphis citricola</i> was reduced approximately 6.0 times, indicating that 3D-PU effectively improved the performance of TSN. The porous 3D-PU can serve as a promising carrier for more pesticide adsorption, encapsulation, safe, highly efficient, and environmentally friendly utilization.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"63 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Cu Fractions on the Light-Induced Spoilage Aromas of Chardonnay Wine at Variable Riboflavin Concentrations
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-02-11 DOI: 10.1021/acs.jafc.4c11032
Isara Vongluanngam, John W. Blackman, Xinyi Zhang, Leigh M. Schmidtke, Kerry L. Wilkinson, Andrew C. Clark
{"title":"Impact of Cu Fractions on the Light-Induced Spoilage Aromas of Chardonnay Wine at Variable Riboflavin Concentrations","authors":"Isara Vongluanngam, John W. Blackman, Xinyi Zhang, Leigh M. Schmidtke, Kerry L. Wilkinson, Andrew C. Clark","doi":"10.1021/acs.jafc.4c11032","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11032","url":null,"abstract":"Despite the known ability of copper(II) to suppress sulfidic aromas, particularly the Cu fractions associated with organic acids and Cu(I) thiol species, the action of these copper fractions on the accumulation of light-induced sulfidic aromas in bottle-aged white wine is unknown. Chardonnay wine, containing different concentrations of riboflavin and copper(II), was exposed to fluorescent light for 6.5 months. The Cu fractions were measured by colorimetry, volatile sulfur compounds by gas chromatography-sulfur chemiluminescence detector (GC-SCD), and volatile aldehydes by gas chromatography-mass spectrometry (GC-MS). The protective Cu fractions inhibited light-induced sulfidic odor accumulation; however, light exposure accelerated the loss of these Cu fractions, and this loss was further accelerated with elevated riboflavin concentration. Upon depletion of the protective Cu fractions, the presence of copper sulfides led to an elevated hydrogen sulfide concentration with further light exposure. The results demonstrated that Cu(II) offers transient protection against the deleterious effects of short-term light exposure but can promote reductive characters with long-term light exposure.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"86 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Self-Assembled PVP-Gd Composite Nanosheets via Ultrasound Synthesis for Targeted Acrylamide Sensing in Food Safety
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-02-11 DOI: 10.1021/acs.jafc.4c08460
Sahar Pakbaten Toopkanloo, Hui-Fen Wu
{"title":"Self-Assembled PVP-Gd Composite Nanosheets via Ultrasound Synthesis for Targeted Acrylamide Sensing in Food Safety","authors":"Sahar Pakbaten Toopkanloo, Hui-Fen Wu","doi":"10.1021/acs.jafc.4c08460","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08460","url":null,"abstract":"Acrylamide (AM) is a recognized carcinogen and neurotoxin, posing a significant threat to food safety and human health. Therefore, developing sensitive and convenient methods for AM detection in food samples is essential. This study responds to the urgent need for sensitive and selective detection of AM, a hidden hazard in food, to safeguard public health and environmental safety. We present the development of a novel two-dimensional ultrasound-synthesized PVP-Gd composite nanosheet platform for precise AM sensing. These self-assembled nanosheets, constructed from gadolinium (Gd) and poly(vinylpyrrolidone) (PVP), exhibit remarkable stability and robust blue fluorescence, with a quantum yield of 45.01% upon excitation at 380 nm. A full factorial design was employed to optimize the synthesis process, revealing significant parameter interactions. The optimized nanosheets demonstrated a strong quenching effect upon acrylamide exposure, resulting in a high-performance acrylamide sensor with an impressively low detection limit (9.4 nM) and a broad linear response range. This innovative sensor platform offers a promising approach for environmental monitoring and food safety applications, effectively addressing the risks associated with acrylamide.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"3 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ethyl-Dimer-Galactopyranoside, a New Glycoside with Attractive Activity from Cultures of Caenorhabditis elegans on NGM
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-02-11 DOI: 10.1021/acs.jafc.4c11812
Tingting Shi, Xiangyu Yang, Keqin Zhang, Pei-Ji Zhao, Guohong Li
{"title":"Ethyl-Dimer-Galactopyranoside, a New Glycoside with Attractive Activity from Cultures of Caenorhabditis elegans on NGM","authors":"Tingting Shi, Xiangyu Yang, Keqin Zhang, Pei-Ji Zhao, Guohong Li","doi":"10.1021/acs.jafc.4c11812","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11812","url":null,"abstract":"The nematode <i>Caenorhabditis elegans</i> is an important model for the study of social behaviors. Ethyl-dimer-galactopyranoside, a new glycoside isolated from the cultures of <i>C. elegans</i> on nematode growth medium (NGM), exhibited strong attractive activity toward <i>C. elegans</i> at different doses, reaching a chemotaxis index of 0.36 at 500 pg. Moreover, the results of transcriptome and chemotaxis assays suggest that ASE neurons are involved in the attractive effect of ethyl-dimer-galactopyranoside toward <i>C. elegans</i>. This study supplemented the understanding of the structure and biological function of modular glycosides of <i>C. elegans</i>. It is noteworthy that the chemotaxis index of 500 pg of ethyl-dimer-galactopyranoside toward <i>Meloidogyne incognita</i> was 0.19. It will also help us to further explore the complex multidirectional communication networks among different nematode species, which may aid the development of new treatment approaches for harmful parasitic nematodes in agriculture.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"29 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Direct Influence of the Conserved Motif in PL7 Family Alginate Lyases on Enzyme Cold Adaptability
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-02-11 DOI: 10.1021/acs.jafc.4c10895
Zhixiao He, Shanshan Meng, Yan Xu, Mingqi Zhong, Xuefeng Han, Qingyi Xie, Mo Ding, Jin Li, Zhong Hu
{"title":"Direct Influence of the Conserved Motif in PL7 Family Alginate Lyases on Enzyme Cold Adaptability","authors":"Zhixiao He, Shanshan Meng, Yan Xu, Mingqi Zhong, Xuefeng Han, Qingyi Xie, Mo Ding, Jin Li, Zhong Hu","doi":"10.1021/acs.jafc.4c10895","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10895","url":null,"abstract":"Alginate lyase, a vital component of polysaccharide lyases, is instrumental in the efficient degradation of alginate and the production of single oligosaccharides. Although numerous alginate lyases have been characterized, only a few display extreme cold adaptability in the range of 0–20 °C. In this study, we identified a novel cold-adapted alginate lyase, Aly423, from <i>Tamlana laminarinivorans</i> PT2-4 isolated from <i>Sargassum</i>. Phylogenetic classification, enzyme structure, and catalytic property analyses confirmed that Aly423 could be classified as a member of subfamily 5 of the PL7 family and exhibited significant cold adaptability at low temperatures. Further analysis of the secondary structure and homology modeling of several cold-adapted enzymes revealed two variable amino acid sites in the conserved amino acid motif (YFK*G*Y) of Aly423, which may affect the cold adaptation mechanism. Point mutation experiments demonstrated that mutant A304T significantly altered the temperature adaptation of Aly423, highlighting the critical role of this amino acid site in the cold-adaptation mechanism of the enzyme. In summary, we effectively enhanced the enzymatic activity of the PL7 alginate cold-adapted enzyme through a rational design using computational methods. This advancement is of significant importance for the efficient utilization of sodium alginate in the food, agricultural, and pharmaceutical industries under low-temperature conditions.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"16 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigating the Olfactory Impact of Monoterpenic Compounds on Fruity Aroma Perception in Model Red Wine Solutions
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-02-10 DOI: 10.1021/acs.jafc.4c11226
Justine Garbay, Sophie Tempere, Lisa Ballet, Jean-Christophe Barbe, Georgia Lytra
{"title":"Investigating the Olfactory Impact of Monoterpenic Compounds on Fruity Aroma Perception in Model Red Wine Solutions","authors":"Justine Garbay, Sophie Tempere, Lisa Ballet, Jean-Christophe Barbe, Georgia Lytra","doi":"10.1021/acs.jafc.4c11226","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11226","url":null,"abstract":"This study investigated the impact of monoterpenic compounds on red wine fruity aroma perception in different matrices and at different concentrations typically found in red wines. Fruity aromatic reconstitutions (FAR) containing sixteen esters that represent the fruity pool of red wine and eight monoterpenic compounds at low, medium, and high concentrations were prepared in dilute alcohol solution (FAR-DAS) and in dearomatized red wine (FAR-DRW). Sensory analysis applied to the olfactory detection threshold of the fruity pool revealed that α-terpineol, at the subthreshold level, and 1,8-cineole, at the suprathreshold level, significantly boosted the olfactory detection threshold of the fruity pool. The results of sensory profiles showed that the presence of a mixture of monoterpenic compounds at a medium concentration in FAR-DAS and in FAR-DRW induced changes in fruity perception. Their addition increased the intensity of black-berry fruit descriptors, such as fresh and jammy blueberry and blackcurrant fruit notes. Moreover, the presence of α-terpineol (130 μg/L), 1,8-cineole (4 μg/L), and linalool (38 μg/L), respectively, increased the fresh raspberry, fresh and jammy blueberry, and jammy blackcurrant notes in FAR-DAS. Across all matrices, the presence of monoterpenic compounds at medium levels consistently amplified black-berry fruit notes. This research provides insights into the impact of monoterpenic compounds on red wine fruity aroma, shedding light on specific enhancing effects of certain monoterpenic compounds via perceptual interactions at various concentrations and in different matrices.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"41 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cyclobutrifluram Resistance in Alternaria alternata: Molecular Mechanisms and Detection Strategies
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-02-10 DOI: 10.1021/acs.jafc.4c10317
Qin Peng, Lijun Tang, Shiqi Tang, Shuai Wang, Jianqiang Miao, Xili Liu
{"title":"Cyclobutrifluram Resistance in Alternaria alternata: Molecular Mechanisms and Detection Strategies","authors":"Qin Peng, Lijun Tang, Shiqi Tang, Shuai Wang, Jianqiang Miao, Xili Liu","doi":"10.1021/acs.jafc.4c10317","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10317","url":null,"abstract":"The resistance risk and mechanisms of cyclobutrifluram in <i>Alternaria alternata</i> are unclear. The baseline sensitivity of 111 <i>A. alternata</i> isolates to cyclobutrifluram was 0.10 ± 0.07 μg/mL. Eight cyclobutrifluram-resistant mutants of <i>A. alternata</i> were generated in the laboratory, exhibiting high-level and relative stable resistance. Cross-resistance was found between cyclobutrifluram and boscalid or pydiflumetofen, yet no cross-resistance was observed between cyclobutrifluram and iprodione, azoxystrobin, mefentrifluconazole, or difenoconazole. Each resistant mutant exhibited a reduced compound fitness index (CFI) compared to their parental isolates. An S73L substitution in AaSdhC or a P113T, H134N, or D145N mutation in AaSdhD conferred cyclobutrifluram resistance. Allele-specific PCR (AS-PCR) methods for the detection of mutations AaSdhC<sup>S73L</sup>, AaSdhD<sup>P113T</sup>, AaSdhD<sup>H134N</sup>, and AaSdhD<sup>D145N</sup> were successfully developed. In summary, <i>A. alternata</i> exhibits a moderate risk for developing resistance to cyclobutrifluram, attributed to the S73L substitution in AaSdhC or the P113T, H134N, or D145N mutations in AaSdhD, which are detectable using specific AS-PCR methods.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dietary Oligosaccharides Isolated from Coix Seed Mitigate Hyperuricemia through Modulation of Lipid Metabolites and Intestinal Homeostasis
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-02-10 DOI: 10.1021/acs.jafc.4c09397
Guozhen Wu, Hongjing Dong, Tao Li, Chuangchuang Wang, Yingjian Guo, Lanping Guo, Xiao Wang
{"title":"Dietary Oligosaccharides Isolated from Coix Seed Mitigate Hyperuricemia through Modulation of Lipid Metabolites and Intestinal Homeostasis","authors":"Guozhen Wu, Hongjing Dong, Tao Li, Chuangchuang Wang, Yingjian Guo, Lanping Guo, Xiao Wang","doi":"10.1021/acs.jafc.4c09397","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09397","url":null,"abstract":"Hyperuricemia (HUA) is a prevalent metabolic disorder associated with chronic disease, posing significant global health challenges. Coix seed, a traditional cereal, has shown therapeutic potential against HUA, with oligosaccharides serving as its primary active components. However, the mechanisms of Coix seed oligosaccharides in HUA management remain underexplored. In this study, a novel oligosaccharide was isolated from Coix seed (CSO) through enzymatic hydrolysis and column chromatography. Structural analysis revealed that the CSO is primarily composed of glucose, with a backbone of →4)-β-Glc<i>p</i>-(1→ linkages. CSO exhibited significant hypouricemic effects in both adenosine-induced HK-2 cells and HUA mice by inhibiting XOD activity and regulating urate transporter expression. Furthermore, CSO restored lipid imbalances, particularly in PS and PC, and modulated gut microbiota by increasing <i>Ruminococcus</i>, <i>Akkermansia</i>, and <i>Lachnospiraceae</i> abundance to alleviate HUA-related systemic disturbances. Importantly, CSO alleviated HUA-induced renal injury by downregulating the IL-6/JAK2/STAT3 signaling pathway. This study provided meaningful evidence supporting the effect of CSO on HUA and offered new directions for natural oligosaccharide interventions in metabolic health.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"51 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening Human IgE Epitopes on α-Lactalbumin and Developing Specific Detection Tools for Enhanced Allergen Detection in Food Matrices
IF 6.1 1区 农林科学
Journal of Agricultural and Food Chemistry Pub Date : 2025-02-10 DOI: 10.1021/acs.jafc.4c11921
Yu Qiu, Mao Ye, Hongkai Tan, Xing Zhang, Huming Shao, Siyi Chen, Yi Zhang, Yong Wu, Jinyan Gao, Xuanyi Meng, Xin Li, Hongbing Chen
{"title":"Screening Human IgE Epitopes on α-Lactalbumin and Developing Specific Detection Tools for Enhanced Allergen Detection in Food Matrices","authors":"Yu Qiu, Mao Ye, Hongkai Tan, Xing Zhang, Huming Shao, Siyi Chen, Yi Zhang, Yong Wu, Jinyan Gao, Xuanyi Meng, Xin Li, Hongbing Chen","doi":"10.1021/acs.jafc.4c11921","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11921","url":null,"abstract":"Food proteins undergo significant structural changes during various processing methods; however, key epitopes often exhibit resistance to damage and modification. Recently, we developed a sensitive sandwich enzyme-linked immunosorbent assay (Epi-mAbs-sELISA) based on IgE linear epitope peptides. This method enables the quantitative detection of alpha-lactalbumin (ALA) for the assessment of food allergenicity. We determined that antibodies developed from IgE linear epitopes possess higher affinity. Ultimately, the optimized Epi-mAbs-sELISA presented in this study demonstrated a wide linear detection range (30.52 ng/mL to 125 μg/mL), a low detection limit (1.122 ng/mL), and excellent precision, with an internal determination relative standard deviation of 9.23% and an external determination relative standard deviation of 9.89%. The recoveries across various food matrices ranged from 81.9% to 111.9%, surpassing the sensitivity and detection range of commercial ELISA kits. The application of this testing method to processed food samples confirmed its ability to detect undeclared ALA residues, thereby mitigating potential safety issues for patients with milk allergies.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"62 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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