Journal of Agricultural and Food Chemistry最新文献_第10页

Lactobacillus reuteri-Enriched Eicosatrienoic Acid Regulates Glucose Homeostasis by Promoting GLP-1 Secretion to Protect Intestinal Barrier Integrity

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-16 DOI: 10.1021/acs.jafc.4c03818

Jun-Xia Wang, Shuang-Yan Chang, Zheng-Yun Jin, Dongxu Li, Jun Zhu, Zhao-Bo Luo, Sheng-Zhong Han, Jin-Dan Kang, Lin-Hu Quan

{"title":"Lactobacillus reuteri-Enriched Eicosatrienoic Acid Regulates Glucose Homeostasis by Promoting GLP-1 Secretion to Protect Intestinal Barrier Integrity","authors":"Jun-Xia Wang, Shuang-Yan Chang, Zheng-Yun Jin, Dongxu Li, Jun Zhu, Zhao-Bo Luo, Sheng-Zhong Han, Jin-Dan Kang, Lin-Hu Quan","doi":"10.1021/acs.jafc.4c03818","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c03818","url":null,"abstract":"Lactobacillus reuteri is a well-known probiotic with beneficial effects, such as anti-insulin resistance, anti-inflammatory, and improvement of the intestinal barrier. However, the underlying mechanisms remain unclear. Here, we found that gavage of L. reuteri improved the intestinal barrier and glucose homeostasis in HFD-fed mice. Analysis of lipid metabolomics reveals a significant increase in eicosatrienoic acid (ETA) levels in mouse feces after L. reuteri gavage. We found that ETA maintain intestinal barrier integrity and improve glucose homeostasis by promoting GLP-1 secretion. Mechanistically, by using CD36 inhibitor in vivo and CD36 knockdown STC-1 cells in vitro, we elucidate that ETA activates intestinal CD36-activated PLC/IP3R/Ca2+ signaling to promote GLP-1 secretion. In vivo administration of GLP-1R inhibitor and in vitro intestinal organoid experiments demonstrate that GLP-1 upregulates the PI3K/AKT/HIF-1α pathway by GLP-1R and increases intestinal tight junction protein expressions, which in turn enhance the intestinal barrier integrity, reduce serum LPS level, attenuate inflammation in white adipose tissue (WAT), and ultimately improve glucose homeostasis in HFD and db/db mice. Our study elucidates for the first time the mechanism by which L. reuteri and its enriched metabolite ETA inhibit WAT inflammation by ameliorating the intestinal barrier, ultimately improving glucose homeostasis, and provides a new treatment strategy for T2D.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of Gut Microbiota by the Complex of Caffeic Acid and Corn Starch

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-13 DOI: 10.1021/acs.jafc.4c06946

Meihui Yu, Nuseybe Bulut, Xinruo Zhao, Rosa Jarumy López Rivera, Yue Li, Bruce R. Hamaker

{"title":"Modulation of Gut Microbiota by the Complex of Caffeic Acid and Corn Starch","authors":"Meihui Yu, Nuseybe Bulut, Xinruo Zhao, Rosa Jarumy López Rivera, Yue Li, Bruce R. Hamaker","doi":"10.1021/acs.jafc.4c06946","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06946","url":null,"abstract":"To understand the impact of different types of polyphenol-starch complexes on digestibility and gut microbiota, caffeic acid (CA) and corn starch (CS) complexes were prepared by coheating and high-pressure homogenization. The resistant starch content in CS coheated with CA (HCS-CA) and HCS-CA after high-pressure homogenization (HCS-CA-HPH) was 47.75 and 56.65%, respectively. Fourier transform infrared spectroscopy and X-ray diffraction analysis revealed hydrogen bonding in coheated samples and enhanced V-complex formation with high-pressure homogenization. The in vitro-digested complexes were of the B + V type, with higher relative crystallinity and short-range ordering of HCS-CA-HPH. Fermentation of the digested complex with human feces increased the yield of acetate, butyrate, and total short-chain fatty acids (SCFAs), which was more pronounced for HCS-CA-HPH. HCS-CA increased torques-Ruminococcaceae abundance, while HCS-CA-HPH boosted Prevotella, Roseburia, Lachnospiraceae, and Lachnospiraceae-NK4A136. Overall, CA and CS complexes enhanced beneficial bacteria and increased SCFA production.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"35 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FM-568: A Promising Phenyl-Hydrazonomalononitrile Antibacterial Agent for the Sustainable Management of Citrus Canker

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-12 DOI: 10.1021/acs.jafc.4c09946

Yixing Gao, Liang Chen, Dongdong Liu, Chunfeng Luo, Jialin Ye, Hongyan Pei, Jing Zhang, Lixin Zhang

{"title":"FM-568: A Promising Phenyl-Hydrazonomalononitrile Antibacterial Agent for the Sustainable Management of Citrus Canker","authors":"Yixing Gao, Liang Chen, Dongdong Liu, Chunfeng Luo, Jialin Ye, Hongyan Pei, Jing Zhang, Lixin Zhang","doi":"10.1021/acs.jafc.4c09946","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09946","url":null,"abstract":"Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), poses a significant threat to citrus production worldwide. To develop effective and eco-friendly antibacterial agents, we designed and synthesized phenyl-hydrazonomalononitrile derivatives using a scaffold-hopping strategy. Among these, FM-568 emerged as a potent candidate, exhibiting broad-spectrum antibacterial activity in vitro against various phytopathogenic bacteria, including Xcc. Greenhouse experiments demonstrated that FM-568 achieved a control efficacy of 88.36% against citrus canker at 400 μg/mL, with an EC50 of 26.68 μg/mL. Field trials in two major citrus-producing regions in China confirmed its effectiveness, yielding control efficacies of 86.60 and 77.87% at 400 μg/mL, outperforming conventional agents like zinc thiazole and thiadiazole copper. Density functional theory calculations suggested that FM-568′s optimized scaffold and electronic properties contribute to its enhanced antibacterial activity. These findings indicate that FM-568 is a promising eco-friendly alternative for managing citrus canker. Further studies on its mechanism of action, safety profile, and formulation optimization are warranted to advance its development for sustainable citrus production.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"118 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and Biochemical Characterization of Olive 4-Hydroxyphenyl Pyruvate Dioxygenase Involved in the Biosynthesis of Tocopherols Present in Virgin Olive Oil

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-12 DOI: 10.1021/acs.jafc.4c06657

Rosario Sánchez, Jesús Expósito Torres, Lourdes García Vico, Pilar Luaces, Carlos Sanz, Ana G. Pérez

{"title":"Molecular and Biochemical Characterization of Olive 4-Hydroxyphenyl Pyruvate Dioxygenase Involved in the Biosynthesis of Tocopherols Present in Virgin Olive Oil","authors":"Rosario Sánchez, Jesús Expósito Torres, Lourdes García Vico, Pilar Luaces, Carlos Sanz, Ana G. Pérez","doi":"10.1021/acs.jafc.4c06657","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06657","url":null,"abstract":"Olive (Olea europaea) fruit contains high amounts of tocopherols that are responsible, along with secoiridoid phenolic compounds, for most of the antioxidant and anti-inflammatory properties of virgin olive oil. This study focuses on the molecular and biochemical characterization of olive 4-hydroxyphenyl pyruvate dioxygenase (OeHPPD) catalyzing the biosynthesis of homogentisic acid, which constitutes the phenolic residue in the tocopherol molecule. OeHPPD is a cytoplasmic enzyme with a molecular weight of 49.8 kDa and a predicted tertiary structure very similar to the Arabidopsis enzyme that suggests similar catalytic mechanisms. OeHPPD has an estimated Kcat of 75.26 s–1 and catalytic efficiency (Km/Kcat) of 0.145 μM–1 s–1 with 4-hydroxyphenyl pyruvate as the substrate. The expression analysis in fruits from selected olive cultivars harvested at different ripening stages indicates that the OeHPPD gene is temporally regulated and cultivar-dependent. Moreover, the analysis of OeHPPD expression in fruits affected by drought stress suggests that HPPD is involved in olive environmental adaptation.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"20 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Throughput Screening for Enhanced Thermal Stability of Inherently Salt-Tolerant l-Glutaminase and Its Efficient Expression in Bacillus licheniformis

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-12 DOI: 10.1021/acs.jafc.4c07745

Yanglu Hu, Hengwei Zhang, Meijuan Xu, Zhiming Rao, Xian Zhang

{"title":"High-Throughput Screening for Enhanced Thermal Stability of Inherently Salt-Tolerant l-Glutaminase and Its Efficient Expression in Bacillus licheniformis","authors":"Yanglu Hu, Hengwei Zhang, Meijuan Xu, Zhiming Rao, Xian Zhang","doi":"10.1021/acs.jafc.4c07745","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07745","url":null,"abstract":"In addressing the challenges posed by extended fermentation cycles and high-salt conditions in high-salt liquid-state fermentation soy sauce (HLFSS) production, a high-throughput screening method was devised to identify thermally stable l-glutaminase mutants. This study yielded mutants A146D and A51D, exhibiting enhanced thermal stability. Computer-aided analysis revealed that these mutations introduced additional forces, compacting the protein structure and lowering the Gibbs free energy, thereby improving thermostability. Furthermore, the incorporation of aspartic acid augmented the negative surface charge, contributing to superior salt tolerance compared to the wild type (WT). Notably, in a 25% NaCl buffer, A146D and A51D demonstrated half-lives of 72.57 and 71.31 day, respectively, surpassing the WT’s 59.08 day. In a 5 L bioreactor, the optimal mutant A146D achieved a remarkable enzymatic activity of 2800.78 ± 98.1 U/mL in recombinant Bacillus licheniformis fermentation broth, setting a new benchmark. This research offers valuable insights and a foundation for the modification and application of l-glutaminase in the food industry, particularly in HLFSS brewing.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"142 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Escherichia coli for Efficient De Novo Synthesis of Salidroside

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-12 DOI: 10.1021/acs.jafc.4c10247

Weizhu Zeng, Huijing Wang, Jianbin Chen, Minglong Hu, Xinru Wang, Jian Chen, Jingwen Zhou

{"title":"Engineering Escherichia coli for Efficient De Novo Synthesis of Salidroside","authors":"Weizhu Zeng, Huijing Wang, Jianbin Chen, Minglong Hu, Xinru Wang, Jian Chen, Jingwen Zhou","doi":"10.1021/acs.jafc.4c10247","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10247","url":null,"abstract":"Salidroside is a high-value plant-derived glycoside with diverse biological activities, but the main industrial salidroside production method, extraction from Rhodiola plants, is insufficient to meet the growing market demand. The biosynthetic route via microbial fermentation is a sustainable and eco-friendly alternative method. De novo synthesis of the precursor tyrosol was established by introducing the ARO10 and ADH6 genes. Systematic metabolic engineering resulted in 3.0 g/L tyrosol, but accumulated tyrosol inhibited cell growth. Adaptive evolution produced an evolved strain with a 10.0% higher OD600 and a 3.3 g/L tyrosol titer. Introducing glucosyltransferase AtUGT85A1, and overexpressing phosphoglucose mutase pgm and UDP-glucose pyrophosphorylase galU, achieved de novo synthesis of salidroside. Furthermore, AtUGT85A1 was semirationally engineered, resulting in the A21G mutation, which enhanced salidroside production by 31.2%. The optimally engineered strain produced 16.8 g/L salidroside with 0.4 g/(L h) productivity in a 5 L bioreactor. This study laid a foundation for future industrial production of salidroside and provided important guidance for efficient biosynthesis of other tyrosol derivatives.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"5 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Context-Dependent Estrogenic Actions of (+)-Pisatin Produced in Elicited Green or Snow Pea (Pisum sativum)

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-12 DOI: 10.1021/acs.jafc.4c06409

Jorge A. Belgodere, Megan C. Benz, G. Wills Kpeli, Jack R. Elliott, Steven Elliott, Jack D. North, Isaac J. Ponder, Peng Ma, Sophie R. Dietrich, Thomas Cheng, Khoa Nguyen, Syreeta L. Tilghman, John A. McLachlan, Binghao Zou, Muralidharan Anbalagan, Brian Rowan, Mark Mondrinos, Thomas E. Wiese, Van T. Hoang, Bridgette M. Collins-Burow, Elizabeth C. Martin, Matthew E. Burow, Stephen M. Boué

{"title":"Context-Dependent Estrogenic Actions of (+)-Pisatin Produced in Elicited Green or Snow Pea (Pisum sativum)","authors":"Jorge A. Belgodere, Megan C. Benz, G. Wills Kpeli, Jack R. Elliott, Steven Elliott, Jack D. North, Isaac J. Ponder, Peng Ma, Sophie R. Dietrich, Thomas Cheng, Khoa Nguyen, Syreeta L. Tilghman, John A. McLachlan, Binghao Zou, Muralidharan Anbalagan, Brian Rowan, Mark Mondrinos, Thomas E. Wiese, Van T. Hoang, Bridgette M. Collins-Burow, Elizabeth C. Martin, Matthew E. Burow, Stephen M. Boué","doi":"10.1021/acs.jafc.4c06409","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06409","url":null,"abstract":"Legumes are a predominant source of isoflavones, termed phytoestrogens, that mimic 17β-estradiol (E2). Phytoalexins are inducible isoflavones produced in plants subjected to environmental stressors (e.g., UV, heat, or fungi). This study investigated estrogenic activity of snow and green peas elicited with Aspergillus sojae. Elicited extracts increased estrogenic activity and proliferation of breast cancer cells (MCF-7 or T47D) in a dose-dependent manner but exhibited antiestrogenic activity when combined with synthetic E2. HPLC analysis of elicited pea extracts identified (+)-pisatin as the primary phytoalexin, which was produced significantly (p < 0.0001) more in snow pea compared to green pea. RNA sequencing results suggested potential functional effects on endothelial cells and tissue vascularization. Indeed, (+)-pisatin enhanced metrics of network assembly and maturation in a microphysiological model of bulk tissue vasculogenesis. Thus, context-dependent functional effects of (+)-pisatin and pharmacologically similar phytoestrogens on the entire tissue microenvironment should be considered in preclinical investigation as potential therapeutic agents.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"9 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142809778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Herbal Multiomics Provide Insights into Gene Discovery and Bioproduction of Triterpenoids by Engineered Microbes

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-12 DOI: 10.1021/acs.jafc.4c08372

Feng Zhang, Xuemi Hao, Jia Liu, Hongping Hou, Shilin Chen, Caixia Wang

{"title":"Herbal Multiomics Provide Insights into Gene Discovery and Bioproduction of Triterpenoids by Engineered Microbes","authors":"Feng Zhang, Xuemi Hao, Jia Liu, Hongping Hou, Shilin Chen, Caixia Wang","doi":"10.1021/acs.jafc.4c08372","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08372","url":null,"abstract":"Triterpenoids are natural products found in plants that exhibit industrial and agricultural importance. Triterpenoids are typically synthesized through two main pathways: the mevalonate (MVA) and methylerythritol 4-phosphate (MEP) pathways. They then undergo structural diversification with the help of squalene cyclases (OSCs), cytochrome P450 monooxygenases (P450s), UDP glycosyltransferases (UGTs), and acyltransferases (ATs). Advances in multiomics technologies for herbal plants have led to the identification of novel triterpenoid biosynthetic pathways. The application of various analytical techniques facilitates the qualitative and quantitative analysis of triterpenoids. Progress in synthetic biology and metabolic engineering has also facilitated the heterologous production of triterpenoids in microorganisms, such as Escherichia coli and Saccharomyces cerevisiae. This review summarizes recent advances in biotechnological approaches aimed at elucidating the complex pathway of triterpenoid biosynthesis. It also discusses the metabolic engineering strategies employed to increase the level of triterpenoid production in chassis cells.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Crystal Structure of Pro c 2 Provides Insights into Cross-Reactivity of Aquatic Allergens from the Phosphagen Kinase Family

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-11 DOI: 10.1021/acs.jafc.4c09727

Yang Yang, Xin-Rong He, Fei Huan, Tian-Liang Bai, Qi-Hui Zhang, Fa-Jie Li, Gui-Xia Chen, Pei-Yi Zheng, Li-Mei Xu, Guang-Ming Liu

{"title":"A Crystal Structure of Pro c 2 Provides Insights into Cross-Reactivity of Aquatic Allergens from the Phosphagen Kinase Family","authors":"Yang Yang, Xin-Rong He, Fei Huan, Tian-Liang Bai, Qi-Hui Zhang, Fa-Jie Li, Gui-Xia Chen, Pei-Yi Zheng, Li-Mei Xu, Guang-Ming Liu","doi":"10.1021/acs.jafc.4c09727","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09727","url":null,"abstract":"Arginine kinase (AK) from the phosphagen kinase family is a cross-reactive shellfish allergen. Structurally related cross-reactive allergens are involved in the pathogenesis of allergic symptoms. This study aimed to unravel the cross-reactivity of AK from a structural perspective. The crystal structure of Procambarus clarkii AK (Pro c 2) was resolved at 1.57 Å resolution, which showed a well-conserved structure not only to shellfish AKs but also to fish creatine kinase (CK), another allergen from the phosphagen kinase family. In Western blot, the CK corresponding protein in fish muscles was found to be reactive with AK-specific immunoglobulin (Ig) G. Recombinant Pro c 2 (rPro c 2) and CKs from Lateolabrax japonicus (rCK-L) and Ctenopharyngodon idell (rCK-C) were then produced, and the IgE reactivity of rCK-L and rCK-C, as well as their IgG/IgE cross-reactivity with rPro c 2, was confirmed by immunological assays. This study demonstrated the cross-reactivity among aquatic allergens from the phosphagen kinase family due to their structural similarity.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"21 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Biofilm to Breath: The Role of Lacticaseibacillus paracasei ET-22 Postbiotics in Combating Oral Malodor. 从生物膜到呼吸：副溶血性乳酸杆菌 ET-22 生后益生菌在消除口腔异味中的作用。

IF 5.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-11 Epub Date: 2024-11-26 DOI: 10.1021/acs.jafc.4c07381

Yue Liu, Bing Fang, Guna Wuri, Hanglian Lan, Ran Wang, Yuhang Sun, Wen Zhao, Wei-Lian Hung, Ming Zhang

{"title":"From Biofilm to Breath: The Role of Lacticaseibacillus paracasei ET-22 Postbiotics in Combating Oral Malodor.","authors":"Yue Liu, Bing Fang, Guna Wuri, Hanglian Lan, Ran Wang, Yuhang Sun, Wen Zhao, Wei-Lian Hung, Ming Zhang","doi":"10.1021/acs.jafc.4c07381","DOIUrl":"10.1021/acs.jafc.4c07381","url":null,"abstract":"Previous studies demonstrated that sufferers with halitosis can be significantly improved with Lacticaseibacillus paracasei ET-22 (ET-22) postbiotics intervention. The objectives of this investigation were to identify the primary components responsible for inhibiting oral malodor. This study demonstrated that cell-free supernatants (CFSs) were more effective in inhibiting production of volatile sulfur compounds (VSCs). Untargeted metabolomics identified CFSs as primarily consisting of organic acids, lipids, peptides, and nucleotides. Among the potential active components, phenyllactic acid (PLA) and peptide GP(Hyp)GAG significantly inhibited microbial-induced VSCs production, with VSC concentrations reduced by 42.7% and 44.6%, respectively. Given the correlation between biofilms and halitosis, microstructural changes in biofilms were examined. PLA suppressed the biomass of the biofilm by 41.7%, while the biofilm thickness was reduced from 202.3 to 70.0 μm. GP(Hyp)GAG intervention reduced the abundance of Fusobacterium nucleatum and Streptococcus mutans within the biofilm, and the expression of biofilm-forming genes FadA and Gtfb were also suppressed by 41.8% and 59.4%. Additionally, the VSC production capacities were reduced due to the decrease in VSC producing bacteria (F. nucleatum, Prevotella intermedia, and Solobacterium moorei) and down-regulation of Cdl and Mgl genes. Collectively, the current study proved that PLA and GP(Hyp)GAG may be the main contributors to halitosis inhibition by ET-22 postbiotics.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":" ","pages":"27203-27214"},"PeriodicalIF":5.7,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0