Journal of Agricultural and Food Chemistry最新文献_第10页

From Aggregation-Prone to Stabilized Whey Protein: Deciphering the Role of Disulfide Bonds and Individual Proteins in Thermal Resistance Ability. 从易于聚集到稳定的乳清蛋白：解读二硫键和单个蛋白质在抗热能力中的作用。

IF 5.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-07-25 DOI: 10.1021/acs.jafc.5c04621

Rui Zhang, Xiaohan Zheng, Guang'ou Chen, Jianyu Zhu, Xiaokang Na, Ming Du, Beiwei Zhu, Chao Wu

{"title":"From Aggregation-Prone to Stabilized Whey Protein: Deciphering the Role of Disulfide Bonds and Individual Proteins in Thermal Resistance Ability.","authors":"Rui Zhang, Xiaohan Zheng, Guang'ou Chen, Jianyu Zhu, Xiaokang Na, Ming Du, Beiwei Zhu, Chao Wu","doi":"10.1021/acs.jafc.5c04621","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c04621","url":null,"abstract":"<p><p>Whey protein (WP) has been gaining popularity in high-protein beverages due to its nutritional advantages. However, its heat-induced aggregation behavior, particularly in salt-containing systems, remains a key barrier to commercialization. Previously, modified whey protein (MWP) with thermostability in the presence of NaCl was synthesized through controlled aggregation. Herein, we disclose the underlying mechanism of how specific protein fractions alter the aggregation behavior of MWP following heating. When heated in NaCl-containing WP system, β-lg, α-la, and BSA all contributed to forming large aggregates via covalent interactions. This was supported by a dramatic decrement in the relative concentration of β-lg and α-la in WP, decreasing from 55% and 30% (before heating) to 2% and 5% (after heating). However, such thermally induced aggregation behavior was limited in MWP, with β-lg and α-la existing predominantly as soluble aggregates. The restricted aggregation behavior was ascribed to the higher absolute zeta potential and disulfide bonds of MWP compared to WP. These features enabled MWP to resist heat-induced structural unfolding, resulting in the formation of smaller aggregates. The disclosure of whey protein aggregation behavior provides theoretical guidance for formulating high-whey protein beverages with tolerance to ionic and temperature changes.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Sensory Analysis Confirms Wood Smoke Exposure as a Source of Smoky Off-Flavors in Fermented Cocoa. 分子感官分析证实木烟暴露是发酵可可烟味的来源。

IF 5.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-07-25 DOI: 10.1021/acs.jafc.5c06046

Franziska Krause, Martin Steinhaus

引用次数: 0

Synbiotic Intervention with Inulin and Lactiplantibacillus plantarum LPm77 Attenuates Type 2 Diabetes via Enhanced TUDCA Metabolism and Gut-Liver Axis Modulation. 菊粉和植物乳杆菌LPm77通过增强TUDCA代谢和肠-肝轴调节的联合干预减轻2型糖尿病。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-07-25 DOI: 10.1021/acs.jafc.5c03515

Minghan Li,Jialin Guo,Jinxin Meng,Xueting Wang,Tianxu Pan,Dongyu Zhao,Hongye Li,Ruyi Gao,Ya Wang,Hechun Li,Songyang Li,Jiagan Wang,Haibin Huang,Nan Wang,Shuyuan Yu,Jiayao Guan,Mingxiao Liu,Chunfeng Wang,Guilian Yang

{"title":"Synbiotic Intervention with Inulin and Lactiplantibacillus plantarum LPm77 Attenuates Type 2 Diabetes via Enhanced TUDCA Metabolism and Gut-Liver Axis Modulation.","authors":"Minghan Li,Jialin Guo,Jinxin Meng,Xueting Wang,Tianxu Pan,Dongyu Zhao,Hongye Li,Ruyi Gao,Ya Wang,Hechun Li,Songyang Li,Jiagan Wang,Haibin Huang,Nan Wang,Shuyuan Yu,Jiayao Guan,Mingxiao Liu,Chunfeng Wang,Guilian Yang","doi":"10.1021/acs.jafc.5c03515","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c03515","url":null,"abstract":"Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by inflammation and immune dysregulation, with the gut-liver axis playing a crucial role in immunometabolic balance. While dietary fibers show therapeutic potential, their mechanisms in T2DM remain unclear. This study investigates the effects of synbiotic intervention combining inulin and Lactiplantibacillus plantarum LPm77 on T2DM, focusing on the gut-liver axis. After 7 weeks of synbiotic treatment, significant improvements in hyperglycemia, insulin resistance, and hepatic steatosis were observed in T2DM mice. Inulin promoted L. plantarum LPm77 colonization, enhancing the gut microbiota balance and restoring the intestinal barrier function. It also regulated bile acid levels in the enterohepatic circulation. The synbiotic promoted tauro-ursodeoxycholic acid (TUDCA) biosynthesis, improving hepatic lipid metabolism and activating TGR5. TGR5 activation suppressed M1 macrophage polarization via the TGR5-TLR4-NF-κB pathway, reducing pro-inflammatory cytokines and increasing IL-10, thus alleviating systemic inflammation. This study highlights a novel therapeutic approach targeting immune-metabolic dysregulation in T2DM.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"119 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous Application of the Functional Metabolite Rosmarinic Acid Can Simulate the Effects of AMF Inoculation on Plant Growth Promotion and Cd Passivation. 外源应用功能代谢物迷迭香酸可以模拟AMF接种对植物生长促进和Cd钝化的作用。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-07-25 DOI: 10.1021/acs.jafc.5c01134

Zhuoyun Zhang,Shuo Liu,Kaili Wang,Yuyang Liu,Hongyu Liu,Fujuan Feng

{"title":"Exogenous Application of the Functional Metabolite Rosmarinic Acid Can Simulate the Effects of AMF Inoculation on Plant Growth Promotion and Cd Passivation.","authors":"Zhuoyun Zhang,Shuo Liu,Kaili Wang,Yuyang Liu,Hongyu Liu,Fujuan Feng","doi":"10.1021/acs.jafc.5c01134","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01134","url":null,"abstract":"Previous studies revealed that inoculation with Glomus mosseae (AMF) significantly increased biomass and reduced cadmium (Cd) accumulation in Cd-sensitive Medicago sativa, which was linked to AMF-induced root exudates recruiting a functional microbiome enriched with plant growth-promoting and Cd-passivating bacteria. Here, we employed microcosm and greenhouse pot experiments to identify key metabolites that enhance the α diversity and functional potential of this microbiome and evaluated their exogenous application effects. Microcosm screening, based on α diversity and functional profiling of growth-promoting and Cd-passivating bacterial communities, identified rosmarinic acid, myristic acid, and indole-3-acetic acid (IAA) as critical functional metabolites. Greenhouse experiments demonstrated that all three metabolites significantly increased plant biomass (rosmarinic acid: 28.8%; myristic acid: 35.8%; IAA: 22.2%; P < 0.05) compared to the control. However, only rosmarinic acid significantly reduced rhizosphere Cd availability (14.4%, P < 0.05), with effects comparable to AMF inoculation (P > 0.05). Single-strain recruitment assays further confirmed that rosmarinic acid exhibited superior capability in enriching functional bacteria, demonstrating stronger chemotaxis and biofilm formation abilities.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"124 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Biodegradation Potential of Enterobacter ludwigii ES2 to Nicosulfuron Using Metabolomics Analysis, Functional Gene El-puuE, and γ-Aminobutyric Acid. 利用代谢组学分析、功能基因El-puuE和γ-氨基丁酸增强路德威肠杆菌ES2对尼科磺隆的生物降解潜力

IF 5.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-07-25 DOI: 10.1021/acs.jafc.5c03977

Yufeng Xiao, Bingbing Yang, Siya Wang, Minghui Zhao, Mei Li, Jian Li, Hao Zhang, Xian Wu

引用次数: 0

Chemoproteomics in Live Bacteria Identifies Covalent Interactome of Epigallocatechin-3-Gallate (EGCG) Underlying Its Multimodal Antibacterial Mechanism. 活细菌的化学蛋白质组学鉴定了表没食子儿茶素-3-没食子酸酯（EGCG）的共价相互作用组及其多模式抗菌机制。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.jafc.5c04484

Shabin N Chathangad,Jissy Anna George,Revathy Sahadevan,Sushabhan Sadhukhan

{"title":"Chemoproteomics in Live Bacteria Identifies Covalent Interactome of Epigallocatechin-3-Gallate (EGCG) Underlying Its Multimodal Antibacterial Mechanism.","authors":"Shabin N Chathangad,Jissy Anna George,Revathy Sahadevan,Sushabhan Sadhukhan","doi":"10.1021/acs.jafc.5c04484","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c04484","url":null,"abstract":"Epigallocatechin-3-gallate (EGCG), the most bioactive catechin of green tea, exhibits remarkable antibacterial activity against a wide range of bacteria, including multidrug-resistant strains. However, its precise molecular targets and mechanisms of action remain poorly understood. Herein, we have designed YnEGCG, a clickable and cell-permeable activity-based probe of EGCG, to comprehensively investigate its molecular targets through in situ chemoproteomics. YnEGCG enabled the fluorescence visualization and mass-spectrometric identification of covalently interacting proteins from live E. coli. Quantitative proteomics identified over 600 proteins, with approximately 10% exhibiting remarkably high enrichment (H:L > 50). These included critical bacterial enzymes such as DNA gyrase, DNA polymerase, ATP synthase, ribosomal proteins, etc., and several previously unidentified targets. Further, we experimentally validated that EGCG inhibits DNA gyrase activity as well as de novo protein synthesis. Taken together, our in situ chemoproteomics studies revealed that EGCG binds to critical bacterial enzymes, uncovering previously unknown antibacterial targets and providing insights into its broad-spectrum action.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"115 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AlTPS6 from Atractylodes lancea Is a Crucial Regulatory Target for Root-Knot Nematode Infestation. 白术AlTPS6是根结线虫侵染的重要调控靶点。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.jafc.5c03252

Xiao Huang,Yuezhen Liu,Lina Chen,Qi Dai,Jin Shang,Juan Deng,Lei Chen,Yuanyuan Chen,Xu Huang,Ling Gong,Kun Yu

{"title":"AlTPS6 from Atractylodes lancea Is a Crucial Regulatory Target for Root-Knot Nematode Infestation.","authors":"Xiao Huang,Yuezhen Liu,Lina Chen,Qi Dai,Jin Shang,Juan Deng,Lei Chen,Yuanyuan Chen,Xu Huang,Ling Gong,Kun Yu","doi":"10.1021/acs.jafc.5c03252","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c03252","url":null,"abstract":"Root-knot nematodes (RKNs) are widespread parasites that damage crops by weakening the plant immune system, causing root galls and stunting growth. However, the molecular mechanisms behind how RKNs regulate host plant defenses remain largely unknown. The medicinal plant Atractylodes lancea frequently suffers losses from RKNs. We investigated the alterations in A. lancea pre- and postinfection by Meloidogyne hapla utilizing histological, transcriptomic, and metabolomic assessment. Infected roots exhibited compromised tissue and giant cell formation as well as a notable reduction of essential oils and four typical bioactive components, i.e., β-eudesmol, hinesol, atractylon, and atractylodin. Transcriptomic analysis revealed significant changes in gene expression related to the cell wall, MAPK pathway, heat shock proteins, peroxidases, ubiquitin proteases, TFs, TPS, and hormones. The AlTPS6 gene was identified as responsible for producing β-caryophyllene, which exhibited strong attraction at low concentrations and nematicidal activity at high concentrations. Taken together, M. hapla downregulated AlTPS6 expression by manipulating host MAPK signaling and related genes to succeed in their infection and reproduction in A. lancea. Our results broaden our insight into the interaction and regulation between RKNs and host.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"8 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caseinolytic Mechanism of Serine Protease MCP746 from Tamarillo (Solanum betacea Cav.) and Potential Application in Fresh Cheese. 柽柳丝氨酸蛋白酶MCP746的酪蛋白溶解机制及其在新鲜奶酪中的潜在应用

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.jafc.5c04993

Tong Zhang,Xinxin Bao,Siying Yang,Chunli Lu,Qiong Zhao,Ying Gu,Shuo Wang

引用次数: 0

Sulforaphane Alleviates PACS 2-Mediated Oxidative Stress Damage in Porcine Endometrial Stromal Cells Induced by Zearalenone. 萝卜硫素可减轻玉米赤霉烯酮诱导的pacs2介导的猪子宫内膜基质细胞氧化应激损伤。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.jafc.5c03839

Chenglu Peng,Sirao Hai,Lei Wang,Zuyan Hu,Xunfei Li,Chang Zhao,Shibin Feng,Wanyue Huang,Xichun Wang

{"title":"Sulforaphane Alleviates PACS 2-Mediated Oxidative Stress Damage in Porcine Endometrial Stromal Cells Induced by Zearalenone.","authors":"Chenglu Peng,Sirao Hai,Lei Wang,Zuyan Hu,Xunfei Li,Chang Zhao,Shibin Feng,Wanyue Huang,Xichun Wang","doi":"10.1021/acs.jafc.5c03839","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c03839","url":null,"abstract":"Zearalenone (ZEA) is commonly found in crops and feed, with a high detection rate and concentration. This substance adversely affects reproduction and development, with particularly noticeable effects on pigs. Given these detrimental effects, there is an urgent need to identify effective protective agents against ZEA toxicity. Sulforaphane (SFN) has emerged as a promising candidate owing to its widely acknowledged antioxidant, anti-inflammatory, and detoxifying properties. This study elucidates ZEA's mechanism of oxidative injury in porcine endometrial stromal cells (ESCs) via mitochondrial-associated membrane (MAM) disruption and demonstrates SFN's protective role. ZEA disrupts the structural integrity and functional dynamics of mitochondrial-associated membranes (MAM) by downregulating PACS 2, leading to elevated intracellular Ca2+ levels (P < 0.01), increased ROS generation (P < 0.01), MDA accumulation (P < 0.01), and suppressed antioxidant enzyme activity (P < 0.01). SFN (10 μM) or the overexpression of PACS 2 can reduce the toxic damage caused by ZEA (45 μM). This study highlights the mechanism by which ZEA causes oxidative damage in porcine cells through mitochondrial membrane disruption and showcases SFN's protective effects, opening up possibilities for broader applications against ZEA toxicity.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Functional Characterization of OsSULTR3;1 in Grain Selenium Accumulation in Rice. 水稻籽粒硒积累中OsSULTR3；1基因的鉴定及功能表征

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.jafc.4c12043

Humphrey Mkumbwa,Ashmit Kumar,Tianyu Mo,Jinwei Qi,Tianhao Wang,Yinglu Sun,Jingjing Fang,Jinfeng Zhao,Rao Muhammad Samran Gul,Hnin Si Htun,Htike Htike Aung,Shoujiang Yuan,Xueyong Li

{"title":"Identification and Functional Characterization of OsSULTR3;1 in Grain Selenium Accumulation in Rice.","authors":"Humphrey Mkumbwa,Ashmit Kumar,Tianyu Mo,Jinwei Qi,Tianhao Wang,Yinglu Sun,Jingjing Fang,Jinfeng Zhao,Rao Muhammad Samran Gul,Hnin Si Htun,Htike Htike Aung,Shoujiang Yuan,Xueyong Li","doi":"10.1021/acs.jafc.4c12043","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c12043","url":null,"abstract":"Rice is a major source of selenium (Se) globally; however, about one billion people experience Se deficiencies. Plants uptake and transport both sulfur (S) and Se using sulfate transporters (SULTRs). Our study aimed to identify rice SULTR genes involved in grain Se accumulation. Expression analysis of 12 rice SULTR genes in tissues at different developmental stages, and under Se and S treatments, revealed that OsSULTR3;1 is strongly expressed in rice grains and mainly induced by Se. Through heterologous expression of OsSULTR3;1 in yeast and the creation of OsSULTR3;1 overexpression and knockout lines, we showed that OsSULTR3;1 is responsible for the active uptake of both selenate and selenite. OsSULTR3;1 knockout and overexpression lines reduced and increased grain Se content by 69.6%, 68.7%, 33.3%, and 78.5%, 15.5%, and 57.5%, respectively, compared with the wild type. Our findings validate the function of OsSULTR3;1 in grain Se accumulation and provide information for breeding Se-enriched rice cultivars.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"17 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0