Journal of Agricultural and Food Chemistry最新文献_第9页

An Ultrasensitive Immunocapture (IC)-RPA-CRISPR/Cas12a Assay with Three Readout Modes for Detecting Xanthomonas oryzae pv. oryzicola of Rice Bacterial Leaf Streak 超灵敏免疫捕获(IC)-RPA-CRISPR/Cas12a三读出模式检测米黄单胞菌水稻细菌性叶条病

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-23 DOI: 10.1021/acs.jafc.5c04360

Shifang Fei, Cui Zhang, Xi Zhang, Yan Xie, Shuai Fu, Jianxiang Wu

{"title":"An Ultrasensitive Immunocapture (IC)-RPA-CRISPR/Cas12a Assay with Three Readout Modes for Detecting Xanthomonas oryzae pv. oryzicola of Rice Bacterial Leaf Streak","authors":"Shifang Fei, Cui Zhang, Xi Zhang, Yan Xie, Shuai Fu, Jianxiang Wu","doi":"10.1021/acs.jafc.5c04360","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c04360","url":null,"abstract":"Xanthomonas oryzae pv oryzicola (Xoc) is the causal agent of rice bacterial leaf streak (BLS) and causes enormous losses of rice yields in many countries every year. Development of sensitive diagnostic techniques is crucial for its prevention and control. Here, we developed an ultrasensitive IC-RPA-CRISPR/Cas12a assay with three readout modes [qPCR machine, UV lamp, and lateral flow strip (LFS)] for Xoc detection in rice, which combined advantages of immunocapture, recombinase polymerase amplification (RPA), and CRISPR/Cas12a-based cleavage. Especially, the immunocapture step allows to capture and enrich Xoc from samples, which minimizes the interference from rice debris to benefit nucleic acid release and amplification and enhances the specificity and sensitivity of this assay. The detection limits of its three readout modes for Xoc bacterial suspension is 2, 6, and 60 CFU/mL, respectively. Collectively, this study provides a specific, ultrasensitive, practical approach for quarantine and detection of Xoc that will benefit the prevention and control of BLS.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"131 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, Synthesis, and Biological Evaluation of Novel Quinoline Derivatives Containing 1,3,4-Oxadiazole Moieties as Potential Antifungal Agents. 含有1,3,4-恶二唑的新型喹啉衍生物的设计、合成和生物学评价。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-23 DOI: 10.1021/acs.jafc.5c06849

Fucheng Yin,Hang Liu,Xinhui Li,Fengyun You,Liu Hu,Yong Guo,Ruige Yang

{"title":"Design, Synthesis, and Biological Evaluation of Novel Quinoline Derivatives Containing 1,3,4-Oxadiazole Moieties as Potential Antifungal Agents.","authors":"Fucheng Yin,Hang Liu,Xinhui Li,Fengyun You,Liu Hu,Yong Guo,Ruige Yang","doi":"10.1021/acs.jafc.5c06849","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c06849","url":null,"abstract":"To discover novel green antifungal candidates, herein, a series of 8-hydroxyquinaldine derivatives were designed and synthesized by introducing the antifungal fragment 1,3,4-oxadiazole. Their antifungal activities against phytopathogenic fungi were evaluated to develop quinoline-based antifungal agents. The results showed that compound VIa6 displayed better in vitro antifungal activity against Valsa mali with an EC50 value of 0.091 μmol/mL. Significantly, compound VIa6 displayed superior in vivo preventive and therapeutic effects against V. mali, comparable to that of Chlorothalonil. Moreover, the combination of VIa6 and Chlorothalonil showed good synergistic antifungal effects. Additionally, compound VIa6 exerted good selectivity and relatively low toxicity to nontarget organisms. Finally, a preliminary antifungal mechanism study indicated that VIa6 affected the viability of fungi by inducing intracellular oxidative damage in V. mali and disrupting the integrity of the fungus cell membranes. These findings demonstrated that the novel quinoline derivative VIa6 possessed excellent in vitro and in vivo antifungal activity, offering the potential as a novel green agricultural antifungal agent.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"35 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145127239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, Synthesis, and Bioactivity of 4-Aryl-1H-naphtho[2,3-d]imidazole Derivatives as Potential Fungicides against Phytopathogenic Fungi 4-芳基- 1h -萘[2,3-d]咪唑衍生物的设计、合成及生物活性研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-23 DOI: 10.1021/acs.jafc.5c06030

Dongdong Chen, Yan Wang, Yujun Wu, Qi Li, Xufeng Yang

{"title":"Design, Synthesis, and Bioactivity of 4-Aryl-1H-naphtho[2,3-d]imidazole Derivatives as Potential Fungicides against Phytopathogenic Fungi","authors":"Dongdong Chen, Yan Wang, Yujun Wu, Qi Li, Xufeng Yang","doi":"10.1021/acs.jafc.5c06030","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c06030","url":null,"abstract":"Inspired by marine natural products like kealiinines A–C, a series of 4-aryl-1H-naphtho[2,3-d]imidazole derivatives were designed and synthesized. The results of their antifungal activities showed that among the twenty-eight synthesized compounds, most of them showed excellent activity against nine common phytopathogenic fungi. Compounds A16, A17, and A20 exhibited remarkable antifungal potency against all fungal strains. Their average EC50 values were 28.1, 11.9, and 14.2 μM, respectively, much lower than that of azoxystrobin (EC50 = 101 μM). A17 can completely control the infection of Colletotrichum gloeosporioides in apples at 37.5 μM over 9 days. Scanning electron microscopy (SEM) observations indicated that A17 can damage the fungal morphology and hyphal structures. Molecular docking results showed that the 4-aryl-1H-naphtho[2,3-d]imidazole scaffold could bind well to succinate dehydrogenase (SDH). The results of seed germination tests indicated that compound A17 had no effect on the germination of cabbage seeds and exhibited favorable safety profiles. SAR analysis demonstrated that the introduction of an aliphatic hydrocarbon to the D ring can enhance activity against the majority of fungi. Therefore, 4-aryl-1H-naphtho[2,3-d]imidazole derivatives have great potential to be developed into novel fungicides.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"35 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Functional Disparity of Pheromone-Binding Proteins from Tomato Leafminer Tuta absoluta in Detection of Sex Pheromone Components 番茄叶螨信息素结合蛋白在性信息素成分检测中的鉴定及功能差异

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-23 DOI: 10.1021/acs.jafc.5c10051

Liu Zhang, Shuo Yang, Jin Xie, Hongwei Li, Wangpeng Shi, Jianfeng Pei, Yanxue Yu, Junfeng Zhai

{"title":"Identification and Functional Disparity of Pheromone-Binding Proteins from Tomato Leafminer Tuta absoluta in Detection of Sex Pheromone Components","authors":"Liu Zhang, Shuo Yang, Jin Xie, Hongwei Li, Wangpeng Shi, Jianfeng Pei, Yanxue Yu, Junfeng Zhai","doi":"10.1021/acs.jafc.5c10051","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c10051","url":null,"abstract":"Studies on the olfactory sensory systems of pests have substantial theoretical potential for the development of eco-friendly control strategies. Tuta absoluta (Meyrick, 1917), one of the most damaging invasive pests of Solanaceae crops, has limited research on its sex pheromone recognition mechanism. In the present study, five PBPs, including three PBP1, PBP2, and PBP3, were identified in T. absoluta. Four TabsPBPs were predominantly expressed in the antennae of male moths, of which TabsPBP1.1 had a strong and similar binding affinity to sex pheromones (3E,8Z,11Z)-tetradecatrien-1-yl acetate (TDTA) and (3E,8Z)-tetradecadien-1-yl acetate (TDDA), and TabsPBP3 had a stronger binding affinity to TDDA than the primary sex pheromone component TDTA. TabsPBP1.3 did not show a sex-biased expression pattern in the antennae, but showed binding affinity to sex pheromones and the host plant volatile terpinolen. Molecular dynamics simulations predicted the key active sites for the binding of TabsPBPs to sex pheromones. TabsPBP1.1/1.3/3 could serve as potential targets for the development of attractants or repellents.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"25 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitation of Polysulfides in Wine, Beer, and Cider by HPLC-MS/MS after Solid-Phase Extraction 固相萃取后HPLC-MS/MS定量测定葡萄酒、啤酒和苹果酒中的多硫化物

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-23 DOI: 10.1021/acs.jafc.5c07483

Yu Hou, Marlize Z. Bekker, Tracey E. Siebert, David W. Jeffery

{"title":"Quantitation of Polysulfides in Wine, Beer, and Cider by HPLC-MS/MS after Solid-Phase Extraction","authors":"Yu Hou, Marlize Z. Bekker, Tracey E. Siebert, David W. Jeffery","doi":"10.1021/acs.jafc.5c07483","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c07483","url":null,"abstract":"Polysulfides are chain-like organosulfur compounds that are recognized as potential reservoirs of volatile sulfur compounds (VSCs) in wine. Quantitative analysis of polysulfides is essential for understanding their relationship with VSCs, so an approach using solid-phase extraction and high-performance liquid chromatography with tandem mass spectrometry was developed, utilizing glutathione di- and trisulfide standards. Quantitation of di- and polysulfides in commercial wine, beer, and cider was then undertaken. Four disulfides were consistently confirmed in beer and wine but were close to the detection limit in ciders. Long-chain polysulfides were detected predominantly in wine samples, with red wines containing higher levels of di- and trisulfides compared to white wines. There was a distinct association between wine parameters of SO2 and pH with the measured sulfur compounds. Principal component analysis revealed some clustering by wine variety, although vintage and region effects were less distinct, highlighting the complex interactions among the factors influencing polysulfide profiles in wine.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"89 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, Synthesis, and Biological Activity Studies of Flavonol Derivatives Containing Pyrazolamide Structure 含吡唑酰胺结构黄酮醇衍生物的设计、合成及生物活性研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-23 DOI: 10.1021/acs.jafc.5c07095

Chunmei Hu, Yujiao Qiu, Dan Shen, Qing Zhou, Fang Tian, Ying Yang, Mingman Sun, Xiujie Yu, Hongyu Zhang, Wei Xue

{"title":"Design, Synthesis, and Biological Activity Studies of Flavonol Derivatives Containing Pyrazolamide Structure","authors":"Chunmei Hu, Yujiao Qiu, Dan Shen, Qing Zhou, Fang Tian, Ying Yang, Mingman Sun, Xiujie Yu, Hongyu Zhang, Wei Xue","doi":"10.1021/acs.jafc.5c07095","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c07095","url":null,"abstract":"A series of flavonol derivatives with pyrazole amide were synthesized, and their antifungal activity was tested against 9 fungi in this study. Most compounds showed strong inhibitory effect against Phytophthora capsici. Notably, J13 exhibited the highest inhibition against Pc (EC50 = 6.29 μg/mL), significantly outperforming azoxystrobin (96.5 μg/mL) and fluopyram (FI, 127.95 μg/mL). In vivo antioomycete experiments with J13 were conducted on chili fruits, leaves, and eggplant. In vivo tests on chili leaves revealed that J13 (200 μg/mL) provided superior curative (89.4%) and protective (87.2%) effects compared to FI (47.9 and 33.7%). J13 caused damage to the cell membranes of Pc hyphae, as observed in scanning electron microscopy (SEM) and fluorescence microscopy (FM) experiments. This membrane disruption was further confirmed by detecting cytoplasmic leakage, assessing membrane permeability, and measuring the malondialdehyde (MDA) content. Furthermore, J13 induced mitochondrial dysfunction, manifested as altered mitochondrial membrane potential and reduced succinate dehydrogenase (SDH) activity; molecular docking and molecular dynamics simulations demonstrated stable binding of J13 to the SDH protein. Moreover, J13 demonstrated favorable biosafety toward both target plants and nontarget organisms. These experimental results lay the groundwork for innovative strategies to develop novel environmentally friendly fungicides.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"61 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Binding Stability and Catalytic Mechanism of a Novel Chitin Deacetylase from Cytobacillus firmus and Preparation of Chitosan 一种新型几丁质脱乙酰酶的结合稳定性、催化机理及壳聚糖的制备。

IF 6.2 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-22 DOI: 10.1021/acs.jafc.5c08532

Bicheng Liang, , , Tong Zhang, , , Guantian Li, , , Haoyue Yang, , , Zongji Wang, , , Kecheng Li, , , Song Liu, , , Huahua Yu, , and , Ronge Xing*,

引用次数: 0

A Novel Nanozyme for Sustainable Agriculture: Improving Wettability, Reducing Toxicity, and Enhancing Drought Resistance in Wheat and Rice 一种用于可持续农业的新型纳米酶：改善小麦和水稻的润湿性、降低毒性和增强抗旱性。

IF 6.2 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-22 DOI: 10.1021/acs.jafc.5c08238

Qianwei Liang, , , Qing Tian, , , Yifei Tan, , , Tianzhen Jiang, , , Zhen Li, , and , Jianguo Feng*,

{"title":"A Novel Nanozyme for Sustainable Agriculture: Improving Wettability, Reducing Toxicity, and Enhancing Drought Resistance in Wheat and Rice","authors":"Qianwei Liang, , , Qing Tian, , , Yifei Tan, , , Tianzhen Jiang, , , Zhen Li, , and , Jianguo Feng*, ","doi":"10.1021/acs.jafc.5c08238","DOIUrl":"10.1021/acs.jafc.5c08238","url":null,"abstract":"Drought stress causes a significant accumulation of reactive oxygen species (ROS) in crops, inhibiting their growth and development and threatening food security. This study constructs cerium-based metal–organic frameworks (CeMs) encapsulated with polydopamine (PDA), effectively eliminating ROS (O2•–, H2O2, ·OH, DPPH·, and ABTS·+). Experiments showed that CeMs@PDA exhibits superior wettability and outstanding translocation capabilities in wheat and rice. Under drought conditions, CeMs@PDA (12.5 mg L–1, 100 mg L–1) significantly increased the vigor index (71.84%, 224.83%) of seeds (wheat, rice). The application of 25 and 100 mg L–1 CeMs@PDA significantly alleviated drought-induced growth inhibition in wheat and rice seedlings, respectively. The inhibition of stem and root growth decreased from 28.07% and 38.15% to 3.72% and 4.43% in wheat seedlings, and the inhibition declined from 25.33% and 30.83% to 2.89% and 5.30% in rice seedlings, respectively. In addition, CeMs@PDA significantly increased the level of antioxidant enzyme activities in wheat and rice and showed better biosafety. This study opens valuable pathways for sustainable agriculture under water-limited conditions.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"73 39","pages":"24622–24633"},"PeriodicalIF":6.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptides IPVP and LPIA Induce Insulin Secretion in a 2-Tiered Caco-2-STC-1/BRIN-BD11 Cell Model 肽IPVP和LPIA诱导Caco-2-STC-1/BRIN-BD11两层细胞模型胰岛素分泌

IF 6.2 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-22 DOI: 10.1021/acs.jafc.5c08240

Maria Cermeño*, , , Marta Santos-Hernández, , and , Richard J. FitzGerald*,

{"title":"Peptides IPVP and LPIA Induce Insulin Secretion in a 2-Tiered Caco-2-STC-1/BRIN-BD11 Cell Model","authors":"Maria Cermeño*, , , Marta Santos-Hernández, , and , Richard J. FitzGerald*, ","doi":"10.1021/acs.jafc.5c08240","DOIUrl":"10.1021/acs.jafc.5c08240","url":null,"abstract":"The insulin release effects of two peptides, IPVP and LPIA, derived from a brewers’ spent grain (BSG) protein hydrolysate, were investigated using a two-tiered Caco-2 and STC-1 (apical: intestinal and enteroendocrine cells, respectively)/BRIN-BD11 (basolateral: pancreatic cells) cell model. Both peptides significantly enhanced insulin secretion in BRIN-BD11 cells (29.64 ± 3.30 and 28.30 ± 1.98 pM insulin for LPIA and IPVP, respectively) following their inclusion on the apical side. However, the direct exposure of BRIN-BD11 cells to peptides did not induce significant changes in insulin secretion, suggesting an indirect mode of action. LPIA significantly increased glucagon-like peptide-1 (GLP-1) levels (43.83 ± 9.25 pM), a known enhancer of insulin release, after 2 h of incubation during Caco-2 and STC-1 cell coculture. Additionally, IPVP and LPIA inhibited dipeptidyl peptidase-IV (DPP-IV) activity in vitro with IC50 values of 38.96 ± 1.26 μM and 31.20 ± 1.15 μM, respectively, and in situ using Caco-2 cells with IC50 values of 58.42 ± 0.45 μM and 59.01 ± 6.54 μM, respectively. The inhibition was via a noncompetitive mixed-type mechanism, and they resisted DPP-IV degradation. These findings highlight the therapeutic potential of IPVP and LPIA in type 2 diabetes management via GLP-1- and DPP-IV-related pathways and warrant further molecular and clinical-level investigations.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"73 39","pages":"24769–24778"},"PeriodicalIF":6.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and Metabolomic Insights into Volatile and Nonvolatile Metabolite Variation from Fresh Leaves to the Turning Stages in Wuyi Rock Tea “Rougui” 武夷岩茶“若桂”鲜叶至转体期挥发性和非挥发性代谢物变异的基因组学和代谢组学研究

IF 6.2 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-22 DOI: 10.1021/acs.jafc.5c06865

Feiquan Wang, , , Huike Li, , , Zongjie Wu, , , Jiawei Yan, , , Hua Feng, , , Ruihua Liu, , , Yao Wu, , , Yiting Liu, , , Wenlong Lei, , , Wenmin Fan, , , Tianmeng Liang, , , Rongping Chen, , , Yingao Zhang, , , Yezi Xiao, , , Xiaomei Lei, , , Bo Zhang*, , , Yucheng Zheng*, , and , Pengjie Wang*,

{"title":"Genomic and Metabolomic Insights into Volatile and Nonvolatile Metabolite Variation from Fresh Leaves to the Turning Stages in Wuyi Rock Tea “Rougui”","authors":"Feiquan Wang, , , Huike Li, , , Zongjie Wu, , , Jiawei Yan, , , Hua Feng, , , Ruihua Liu, , , Yao Wu, , , Yiting Liu, , , Wenlong Lei, , , Wenmin Fan, , , Tianmeng Liang, , , Rongping Chen, , , Yingao Zhang, , , Yezi Xiao, , , Xiaomei Lei, , , Bo Zhang*, , , Yucheng Zheng*, , and , Pengjie Wang*, ","doi":"10.1021/acs.jafc.5c06865","DOIUrl":"10.1021/acs.jafc.5c06865","url":null,"abstract":"Wuyi rock tea is renowned for its distinctive ″rock flavor,″ largely formed during the turning stage through mechanical stress and enzymatic changes.To investigate its molecular basis, we assembled the most contiguous tea genome to date from the representative cultivar “Rougui” and identified structural variations distributed across flavor-related genes and pathways. Metabolomic profiling highlighted the fourth and fifth turning stages as critical, showing surges in volatiles and nucleotides, rapid catechin and amino acid degradation, and a striking 180-fold rise in theaflavins. Leveraging transcriptome data with >97% mapping rate to the “Rougui” genome, we identified 1248 genes forming key coexpression modules associated with volatiles and theaflavins biosynthesis. These modules include 10 TPSs, PPO, POD, and multiple upstream structural genes that were coordinately activated during the sequential turning stages. The results offer genomic resources and insights into the regulation of flavor formation in Wuyi rock tea.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"73 39","pages":"25089–25102"},"PeriodicalIF":6.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0