Journal of Agricultural and Food Chemistry最新文献_第9页

Microwave Processing Alters Milk Protein Digestion and Peptide Release: Insights from Peptidomics and Bioactivity Evaluation 微波处理改变牛奶蛋白消化和肽释放：来自肽组学和生物活性评价的见解

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-10-05 DOI: 10.1021/acs.jafc.6c09292

Yaxin Huang, Yuan Tao, Huayu Yang, Bowen Yan, Nana Zhang, Bo Hu, Hao Zhang, Wei Chen, Daming Fan

引用次数: 0

The Critical Role of Molecular Weight in Digestion Stability and Gut Microbiota-Dependent Fermentation of Dendrobium officinale Polysaccharides 分子量在铁皮石斛多糖消化稳定性和肠道微生物依赖发酵中的关键作用

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-10-05 DOI: 10.1021/acs.jafc.6c10562

Jinfeng Fu, Pingping Wang, Chun Chen, Jiansheng Cheng, Yu Xie, Xiong Fu

{"title":"The Critical Role of Molecular Weight in Digestion Stability and Gut Microbiota-Dependent Fermentation of Dendrobium officinale Polysaccharides","authors":"Jinfeng Fu, Pingping Wang, Chun Chen, Jiansheng Cheng, Yu Xie, Xiong Fu","doi":"10.1021/acs.jafc.6c10562","DOIUrl":"10.1021/acs.jafc.6c10562","url":null,"abstract":"This study systematically investigated the impact ofmolecularweight (Mw) on the gastrointestinal fate and gut microbiota modulationof Dendrobium officinale polysaccharides(DOP). A series of DOPs with different Mw (509–110 kDa) wasobtained via ultrasonic degradation. During simulated digestion, allDOPs exhibited partial depolymerization, evidenced by reduced Mw andincreased reducing sugar content, with high-Mw DOPs being more susceptibleto gastric conditions. Although the triple-helical structure and microtopographywere disrupted, the functional groups and biological activity remainedlargely intact. DOPs were extensively utilized by gut microbiota inan Mw-dependent manner. Notably, low-Mw UDOP-9 preferentially promotedthe proliferation of Bacteroides (up to 29.04%) and Parabacteroides, whereas high-Mw DOP selectively enhanced Bifidobacterium growth. All DOPs significantly increasedshort-chain fatty acid (SCFAs) production, particularly acetate. However,Mw did not affect the total SCFAs content. These findings revealedthe important role of Mw in regulating polysaccharide digestion andthe gut microbiota.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31731–31747"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, Fungicidal Activity, and Action Mode of Functionalized Siderophores 功能化铁载体的合成、杀真菌活性和作用方式

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-10-02 DOI: 10.1021/acs.jafc.6c09445

Minghui Chen, Sai Hong, Xuemin Wu, Yuxia Hou, Yumei Xiao, Bin Fu, Jiaqi Li, Zhaohai Qin

{"title":"Synthesis, Fungicidal Activity, and Action Mode of Functionalized Siderophores","authors":"Minghui Chen, Sai Hong, Xuemin Wu, Yuxia Hou, Yumei Xiao, Bin Fu, Jiaqi Li, Zhaohai Qin","doi":"10.1021/acs.jafc.6c09445","DOIUrl":"10.1021/acs.jafc.6c09445","url":null,"abstract":"Iron homeostasis influences fungalpathogenicity and is a promisingtarget for crop protection. In this study, catechol moieties wereincorporated into G2-class fungicidal scaffolds to prepare a seriesof siderophores. At 50 μg/mL, the compounds PP-3 and PPDM-2 achieved mean inhibition rates (MIRs) of64.0% and 67.2%, respectively, against 11 plant pathogens, comparableto tridemorph (64.6%). However, the two compounds exhibited distinctactivity spectra. PP-3 and PPDM-2 showedstrong activity against two oomycete pathogens (Pythium aphanidermatum and Phytophthora capsici) and Fusarium graminearum, suggestingpotential for crop disease management. Preliminary assays indicateddisruption of Fe3+/Fe2+ homeostasis and rapidion leakage through nonspecific membrane lipid perturbation; inhibitionof ergosterol biosynthesis may additionally contribute to activityagainst fungi. These findings support the development of agriculturalfungicides incorporating functional iron-chelating moieties.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31503–31518"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fucoidan Alleviates Intestinal Inflammation through Microbiota-Associated Citraconic Acid Accumulation to Modulate Ferroptosis-Related Pathways 岩藻糖聚糖通过菌群相关的柠檬酸积累调节凋亡相关途径减轻肠道炎症

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-09-30 DOI: 10.1021/acs.jafc.6c08698

Yifan Wei, Xiaomeng Ren, Shuang Song, Chunqing Ai

{"title":"Fucoidan Alleviates Intestinal Inflammation through Microbiota-Associated Citraconic Acid Accumulation to Modulate Ferroptosis-Related Pathways","authors":"Yifan Wei, Xiaomeng Ren, Shuang Song, Chunqing Ai","doi":"10.1021/acs.jafc.6c08698","DOIUrl":"10.1021/acs.jafc.6c08698","url":null,"abstract":"Fucoidan has shown potential forintestinal protection;however,its underlying mechanisms remain unclear. This study investigatedthe protective effects of fucoidan against DSS-induced colitis andexplored microbiota-associated metabolic changes that were linkedto its protective effects. Fucoidan alleviated colonic injury, improvedepithelial barrier integrity, and reshaped the gut microbial profile,including reduced Pseudomonadota levels and the enrichment of beneficialtaxa. Targeted metabolomics identified citraconic acid (CA) as a candidatemetabolite associated with fucoidan-mediated protection. Antibioticdepletion suggested that fucoidan-associated CA accumulation was partiallydependent on the gut microbiota. CA reduced colitis severity, attenuatedferroptosis-related oxidative stress, and improved barrier function,accompanied by enhanced Nrf2-associated antioxidant responses andthe restoration of SLC7A11 and GPX4 expression. In vitro, CA reducedmitochondrial Fe2+ accumulation and lipid peroxidationwhile enhancing tight junction protein expression. These results highlighta microbiota-associated metabolic mechanism underlying fucoidan-mediatedintestinal protection and identify CA as a potential mediator of itsanti-inflammatory activity.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31715–31730"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eupatilin Attenuates Mastitis in Dairy Cows by Activating the NRF2 Pathway 尤帕汀通过激活NRF2通路减轻奶牛乳腺炎

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-10-02 DOI: 10.1021/acs.jafc.6c02196

Yiyi Zhao, Qingqing Zhou, Xinyuan Zhang, Gefen Yin, Junlong Bi, Jie Hou, Xiaobing Li, Zhiyuan Fang

{"title":"Eupatilin Attenuates Mastitis in Dairy Cows by Activating the NRF2 Pathway","authors":"Yiyi Zhao, Qingqing Zhou, Xinyuan Zhang, Gefen Yin, Junlong Bi, Jie Hou, Xiaobing Li, Zhiyuan Fang","doi":"10.1021/acs.jafc.6c02196","DOIUrl":"10.1021/acs.jafc.6c02196","url":null,"abstract":"Antibiotic treatment of mastitis in dairy cows can causeantibioticresidues and antimicrobial resistance, highlighting the urgent needfor eco-friendly and sustainable alternatives. Here, we show thatdairy cows with mastitis have severe inflammation, apoptosis, andoxidative stress in mammary tissue. Eupatilin (EU), a natural compoundderived from Artemisia argyi, alleviatedLPS-induced pathological damage in bovine mammary epithelial cells(BMECs) by enhancing the transcriptional activity of NRF2. Knockdownof NRF2 abolished the protective effects of EU againstLPS-induced inflammation, apoptosis, and oxidative stress in BMECs,whereas overexpression of NRF2 did not further enhancethese effects. Importantly, the protective effect of EU against LPS-inducedmastitis in mice was fully reversed by pharmacological inhibitionof NRF2. This work not only identifies NRF2 as a potential therapeutictarget for combating mastitis but also identifies EU as a promisingtherapeutic agent and an effective non-antibiotic option for the prophylaxisand therapy of mastitis.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31568–31582"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolite-GuidedRegulation of the Rhizosphere Microbiomeby a Synthetic Microbial Consortium and Key Mixed Metabolites SuppressesRoot Rot Disease in Fritillaria pallidiflora 代谢物引导的根际微生物群调控：合成微生物联合体和关键混合代谢物抑制白贝母根腐病

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 DOI: 10.1021/acs.jafc.6c11017

Guang Yang,Jianrui Dong,Bing Qin,Xiaodan Zhang,Yanyan Xu,Hegan Dong,Wenqin Zhao

{"title":"Metabolite-Guided\u0000Regulation of the Rhizosphere Microbiome\u0000by a Synthetic Microbial Consortium and Key Mixed Metabolites Suppresses\u0000Root Rot Disease in\u0000 Fritillaria pallidiflora","authors":"Guang Yang,Jianrui Dong,Bing Qin,Xiaodan Zhang,Yanyan Xu,Hegan Dong,Wenqin Zhao","doi":"10.1021/acs.jafc.6c11017","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c11017","url":null,"abstract":"Abstract Continuous cropping can weaken soil disease suppression, but its microbial and metabolic bases remain unclear. We compared first-planting and replant rhizospheres of Fritillaria pallidiflora at 2-, 3-, and 4-year cultivation stages sampled in different calendar years using soil analysis, sequencing, metabolomics, and pot experiments. Replant soils had a higher root rot incidence, greater Fusarium solani abundance, and lower microbial network complexity and stability. Nitrate, ammonium, and electrical conductivities were associated with community restructuring. Five metabolites were linked to disease incidence and microbial taxa. A synthetic community (SynCom) of four Bacillus strains and one Brevibacillus laterosporus strain reduced F. solani abundance and disease incidence, enriched beneficial bacteria, increased antioxidant enzyme activities, and promoted growth. All five strains were recovered from the rhizosphere after 30 days. Combining the SynCom with the five metabolites produced the strongest disease suppression and plant growth. These findings support the evaluation of metabolite-guided microbial consortia for disease management.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"80 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut–MammaryAxis-Mediated Improvement of SialylatedMilk Oligosaccharide Synthesis by Polygonatum sibiricum Polysaccharide and Probiotics in Mice with Gestational MetabolicDisorders 肠道-乳腺轴介导的西伯利亚黄精多糖和益生菌对妊娠期代谢紊乱小鼠唾液化乳寡糖合成的影响

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 DOI: 10.1021/acs.jafc.6c08273

Ye Wang,Yan-Ling Kang,Bin-Qi Rui,Jing Zhao,Man-Lin Zhou,Qi-Yu E,Bushra Walayat,Tong Pan,Yi Zheng,Xiao-Ming Zhang,Yin-Hui Liu,Jing-Yu Yan,Ming Li

{"title":"Gut–Mammary\u0000Axis-Mediated Improvement of Sialylated\u0000Milk Oligosaccharide Synthesis by\u0000 Polygonatum sibiricum\u0000 Polysaccharide and Probiotics in Mice with Gestational Metabolic\u0000Disorders","authors":"Ye Wang,Yan-Ling Kang,Bin-Qi Rui,Jing Zhao,Man-Lin Zhou,Qi-Yu E,Bushra Walayat,Tong Pan,Yi Zheng,Xiao-Ming Zhang,Yin-Hui Liu,Jing-Yu Yan,Ming Li","doi":"10.1021/acs.jafc.6c08273","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c08273","url":null,"abstract":"Abstract Milk oligosaccharides (MOs) are vital for infant health but vulnerable to gestational metabolic dysfunction. To explore a new microecological intervention strategy, we established a probiotic fermentation platform coupled with a mammary epithelial cell screening model to identify Polygonatum sibiricum polysaccharide (PSP) as a promising candidate. Murine models demonstrated that both PSP monotherapy and synergistic treatment with probiotics significantly mitigated gestational metabolic dysregulation and restored postpartum sialylated MOs. Mechanistically, the combined treatment outperformed single interventions by modulating glycerophospholipid (GPL) and polyunsaturated fatty acid (PUFA) metabolism, mitigating mammary gland mitochondrial dysfunction and ATP deficiency, and significantly upregulating the mRNA expression of sialyltransferase gene ST3GAL1. Notably, ATP restoration improved precursor availability, while ST3GAL1 upregulation promoted terminal sialylation, jointly facilitating sialylated MO biosynthesis. Our findings provide novel insights into how maternal metabolic health governs sialylated MO biosynthesis via the gut–mammary gland axis and propose a synergistic microecological intervention strategy for gestational metabolic disorders.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"14 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Functional Analysis of the CLAVATA3/embryo Surrounding Region-Related (CLE) Gene Family in Tartary Buckwheat Suggests the Promoting Effect of FtCLE7 on Growth and Nitrogen Accumulation 苦荞CLAVATA3/胚胎周边区域相关（CLE）基因家族的鉴定和功能分析提示FtCLE7对生长和氮素积累有促进作用

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-10-01 DOI: 10.1021/acs.jafc.6c07923

Changying Liu, Yaoxuan Zou, Jiting Wang, Hanlin Wang, Qi Wu, Sha Zheng, Xiaoqin Zheng, Maoling Tan, Xi Wu, Daiying Xu, Junjie Yin, Yan Wan, Linsen Mei, Dabing Xiang, Wenjun Sun

{"title":"Identification and Functional Analysis of the CLAVATA3/embryo Surrounding Region-Related (CLE) Gene Family in Tartary Buckwheat Suggests the Promoting Effect of FtCLE7 on Growth and Nitrogen Accumulation","authors":"Changying Liu, Yaoxuan Zou, Jiting Wang, Hanlin Wang, Qi Wu, Sha Zheng, Xiaoqin Zheng, Maoling Tan, Xi Wu, Daiying Xu, Junjie Yin, Yan Wan, Linsen Mei, Dabing Xiang, Wenjun Sun","doi":"10.1021/acs.jafc.6c07923","DOIUrl":"10.1021/acs.jafc.6c07923","url":null,"abstract":"Low nitrogen use efficiency caused by irrational nitrogenfertilizationrestricts Tartary buckwheat (TB) yield improvement. CLE peptides arekey signaling molecules regulating plant nitrogen response, yet theirfunctions in TB remain unclear. Herein, 16 FtCLE geneswere identified in TB, and all encoding proteins contained conservedbioactive CLE mature peptides. The root-specific and high nitrogen-inducedgene, FtCLE7, was screened as the key CLE member by expression analysis. Exogenous synthetic FtCLE7 peptidetreatment significantly promoted growth and nitrogen accumulationin TB, and reshaped rhizosphere microbial communities. Transcriptomeanalysis demonstrated that FtCLE7 peptide changed the expression ofgenes involved in hormone signaling, phenylpropanoid biosynthesis,transcription regulation, and receptor-like kinases. Additionally,overexpression of FtCLE7 in tobacco verified itspositive regulatory role in growth and nitrogen accumulation. Thisstudy clarifies the biological function of FtCLE7 in modulating nitrogen accumulation, providing a theoretical basisfor breeding nitrogen-efficient TB varieties and developing novelpeptide fertilizers.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31462–31477"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rice Bran Insoluble Dietary Fiber and Its Bound Polyphenols Alleviate Colitis by Regulating the Autophagy-NLRP3 Inflammasome Axis 米糠不溶性膳食纤维及其结合的多酚通过调节自噬- nlrp3炎性小体轴缓解结肠炎

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-10-01 DOI: 10.1021/acs.jafc.6c06416

Li Liu, Bing Zheng, Hui Ye, Jianhua Xie, Yi Chen, Xiaole Zhao, Yufei Hu, Xiaobo Hu, Qiang Yu

{"title":"Rice Bran Insoluble Dietary Fiber and Its Bound Polyphenols Alleviate Colitis by Regulating the Autophagy-NLRP3 Inflammasome Axis","authors":"Li Liu, Bing Zheng, Hui Ye, Jianhua Xie, Yi Chen, Xiaole Zhao, Yufei Hu, Xiaobo Hu, Qiang Yu","doi":"10.1021/acs.jafc.6c06416","DOIUrl":"10.1021/acs.jafc.6c06416","url":null,"abstract":"Although dietary fiber alleviates colonic inflammation,most studieshave focused on carbohydrate components, neglecting bound polyphenols(BPs). Rice bran insoluble dietary fiber (RIDF), rich in BPs, improvescolitis; however, how BPs work and their specific contributions remainelusive. Our study demonstrates that BPs are important contributorsof RIDF in alleviating colitis. Relative to rice bran dietary fiberwithout BPs (RIDF_DF), RIDF markedly ameliorated colitis symptoms,mitigated endoplasmic reticulum stress (ER stress), restored the gutmicrobiome and metabolites, and blocked the PI3K/Akt/mTOR cascadeand NLRP3 inflammasome activation. 3-MA abolished, whereas RAPA restored,these protective effects via the autophagy-NLRP3 axis, identifyingthis axis as an important hub. Collectively, BPs served as an importantfunctional component of RIDF. They ameliorated colitis by activatingthe autophagy-NLRP3 axis, which suppressed ER stress, repaired theintestinal barrier, and restored the gut microbiome and metabolome.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31647–31665"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycitin Targets the Orphan Receptor GPR3 to Promote Milk Fat Synthesis in Goat Mammary Epithelial Cells 甘油三酯靶向孤儿受体GPR3促进山羊乳腺上皮细胞乳脂合成

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-09-29 DOI: 10.1021/acs.jafc.6c00383

Liaoyu Fan, Chu Chen, Xiaoran Li, Runyu Yang, Hua Wang, Huaiping Shi

引用次数: 0