IF 4.6 1区农林科学

Food microbiology Pub Date : 2026-01-01 Epub Date: 2025-07-30 DOI: 10.1016/j.fm.2025.104882

Ksenia Mozhaitseva, Sylvie Pairaud, Olivier Firmesse, Mathilde Bonis

{"title":"Population structure of Bacillus cereus sensu lato associated with foodborne outbreaks in France between 2004 and 2023.","authors":"Ksenia Mozhaitseva, Sylvie Pairaud, Olivier Firmesse, Mathilde Bonis","doi":"10.1016/j.fm.2025.104882","DOIUrl":"https://doi.org/10.1016/j.fm.2025.104882","url":null,"abstract":"Bacillus cereus sensu lato (Bcsl) is a group of closely related bacterial species known for their resistant spores, enabling them to persist in a dormant state and thereby colonize and adapt across diverse environments. Bcsl is known for its harmful impact on human health, producing toxins that cause emetic and diarrheal syndromes or provoking extradigestive infections. Importantly, Bcsl is the most frequent confirmed or presumptive causative agent associated with foodborne outbreaks (FBOs) in France. In our study, we assessed the population structure of a large collection of Bcsl isolated during FBOs investigation in France between 2004 and 2023, focusing on the association between distinct populations and food categories. Using 294 genomes from 183 FBOs, we applied genomic clustering and phylogenomic analysis and then identified three predominant Bcsl populations. B. cereus sensu stricto (17.0 %) prevailed in composite dishes, B. paranthracis (16.1 %) was positively associated with cereals, and B. thuringiensis subsp. kurstaki (7.6 %) was predominantly found in vegetable-based salads. Some strains were phylogenetically closely related to clinical isolates, highlighting the need to assess the antibiotic susceptibility of Bcsl. Notably, one Bcsl clade, B. cytotoxicus, lacking beta-lactamase-encoding genes showed a greatly increased sensitivity to ampicillin than other Bcsl considered to be naturally resistant to beta-lactams. Additionally, various strains from distinct populations showed reduced susceptibility to macrolides and cyclins. Finally, accurately differentiated populations will be used in further epidemiological studies and in dose-response modeling.","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"133 ","pages":"104882"},"PeriodicalIF":4.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of physiological transitions during forward processing on Shiga-toxin producing Escherichia coli risks in lettuce. 正加工过程中生理变化对生菜产志贺毒素大肠杆菌风险的影响。

IF 4.6 1区农林科学

Food microbiology Pub Date : 2026-01-01 Epub Date: 2025-08-06 DOI: 10.1016/j.fm.2025.104892

Joshua Ombaka Owade, Teresa M Bergholz, Jade Mitchell

{"title":"Impact of physiological transitions during forward processing on Shiga-toxin producing Escherichia coli risks in lettuce.","authors":"Joshua Ombaka Owade, Teresa M Bergholz, Jade Mitchell","doi":"10.1016/j.fm.2025.104892","DOIUrl":"https://doi.org/10.1016/j.fm.2025.104892","url":null,"abstract":"Cold stress during forward processing delays of lettuce can induce the formation of viable but nonculturable (VBNC) cells of Shiga toxin-producing Escherichia coli (STEC) O157:H7 and pose risks of foodborne disease outbreaks. This study investigated the effect of physiological changes during the forward processing cold chain on the risks of illness from consuming lettuce contaminated with STEC O157:H7. A probabilistic quantitative microbial risk assessment model was developed to quantify the risks associated with consuming field-bagged Romaine hearts and shredded and packaged lettuce contaminated with STEC O157:H7. The exposure assessment included the farm-to-consumer pathway, with probability distributions generated using 105 Monte Carlo simulations. The risk of illness was calculated using a previously published beta-Poisson model. Scenario analysis was conducted to account for transition to VBNC over 5 days of cold storage. The median risk of consuming field-bagged Romaine hearts and shredded and packaged lettuce was 1.88×10-8 (95 % CI = 1.59×10-11; 4.97×10-4) and 9.12×10-7 (95 % CI = 2.41×10-8, 3.90×10-5), respectively. Convolution tests showed the distribution of risks of consuming Romaine hearts and shredded and packaged lettuce were not significantly (p > 0.05) different. Physiological changes due to cold stress during forward processing did not significantly increase the risk of illness for either field-bagged Romaine hearts or shredded and packaged lettuce (p > 0.05). While post-processing factors were the most important uncertainty factors influencing the risks from shredded and packaged lettuce, both pre- and postharvest factors most influenced the risks from field-bagged Romaine hearts. We concluded that cold stress along the lettuce distribution chain, despite inducing physiological changes in the cells, did not significantly increase the risks of illness.","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"133 ","pages":"104892"},"PeriodicalIF":4.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tomato leaf microstructure affects the adhesion and localization of Salmonella enterica as shown using biomimetics. 仿生学显示，番茄叶片微观结构影响肠沙门氏菌的粘附和定位。

IF 4.6 1区农林科学

Food microbiology Pub Date : 2026-01-01 Epub Date: 2025-08-07 DOI: 10.1016/j.fm.2025.104893

Orian Dayan, Yulia Kroupitski, Tali Sayas, Shlomo Sela Saldinger, Maya Kleiman

{"title":"Tomato leaf microstructure affects the adhesion and localization of Salmonella enterica as shown using biomimetics.","authors":"Orian Dayan, Yulia Kroupitski, Tali Sayas, Shlomo Sela Saldinger, Maya Kleiman","doi":"10.1016/j.fm.2025.104893","DOIUrl":"https://doi.org/10.1016/j.fm.2025.104893","url":null,"abstract":"Non-typhoidal Salmonella enterica serovars are a major cause of diarrheal diseases worldwide and represent a significant health concern. Several Salmonella outbreaks worldwide originated from bacteria residing on plants, specifically on leaves. Understanding the adhesion and survival of Salmonella upon the leaf surface is, hence, of great importance. Among other factors involved in the localization and adhesion of Salmonella to the leaf surface, the surface microstructure did not receive significant attention. Here, we study the localization and adhesion of Salmonella to the surface of tomato leaves, with emphasis on the role of the leaf surface microstructure. To do so, we use biomimetics, a field in chemistry and material sciences aimed at mimicking biological systems. We formed synthetic replication of the leaf surface microstructure, to isolate the microstructure property from all other leaf properties. We found that the distribution of Salmonella upon the leaf surface is not random and there is a clear localization preference to the intercellular spaces and the trichomes. We found that this localization repeats in the synthetic system, suggesting this phenomenon is due to the microstructural features of the leaf. The localization of Salmonella on the trichome is independent of flagella, curli or cellulose, and does not require bacterial viability. However, the overall adhesion of Salmonella to both natural and synthetic leaf surfaces decreased in the cellulose mutant. This result emphasizes the strength of the model synthetic system we developed. A better understanding of Salmonella interaction with leaf surfaces could yield new directions for prevention methods. The findings in this research could assist in the development of such directions.","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"133 ","pages":"104893"},"PeriodicalIF":4.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-phase Hog1 activation and transporter gene reprogramming enable extreme sugar tolerance in food osmophilic yeasts. 双相Hog1激活和转运体基因重编程使食物嗜渗酵母具有极强的糖耐受性。

IF 4.6 1区农林科学

Food microbiology Pub Date : 2026-01-01 Epub Date: 2025-07-21 DOI: 10.1016/j.fm.2025.104879

Hong Guo, Qi Wang, Wenxi Lv, Yuxiang Zhang, Fei Wang, Yahong Yuan, Tianli Yue

{"title":"Dual-phase Hog1 activation and transporter gene reprogramming enable extreme sugar tolerance in food osmophilic yeasts.","authors":"Hong Guo, Qi Wang, Wenxi Lv, Yuxiang Zhang, Fei Wang, Yahong Yuan, Tianli Yue","doi":"10.1016/j.fm.2025.104879","DOIUrl":"https://doi.org/10.1016/j.fm.2025.104879","url":null,"abstract":"The protein kinase Hog1 plays a central role in cellular responses, including cell volume and gene expression regulation during osmoregulation in the model yeast Saccharomyces cerevisiae. Despite sharing the conserved kinase Hog1 for osmotic response, Zygosaccharomyces rouxii and S. cerevisiae exhibit markedly different sugar resistance. Here, we systematically compared the phenotypes, Hog1 phosphorylation kinetics, and transcriptomic profiles of both yeasts under 60 % (w/v) extremely high-glucose stress. Under 60 % (w/v) extremely high-glucose stress, Z. rouxii exhibits prolonged survival with volume recovery post-shrinkage, contrasting S. cerevisiae's irreversible collapse. Additionally, we found that the important Hog1 kinase shows transient activation with Hsp70-coupled recovery in Z. rouxii versus sustained activation in S. cerevisiae. Correspondingly, transcriptome data showed different expression patterns of transmembrane transport differentially expressed genes (DEGs): S. cerevisiae upregulated high-affinity transporter genes (HXT3: 5.2-fold; HXT4: 4.7-fold), whereas Z. rouxii induced low-affinity transporter genes (ZYRO0E10054 (FFZ1): 1.6-fold; ZYRO0F02090 (FFZ2): 25.8-fold) under 60 % (w/v) extremely high-glucose stress. Most transmembrane transport gene expression patterns persist in 60 °brix apple juice stress (complex sugar), except for stress-type-specific induction of ZYRO0F02090 (FFZ2) and ZYRO0E09988 (FLR1). Our work deciphers the evolutionary divergence of sugar osmoadaptation strategies in yeasts, providing actionable targets for engineering microbial sugar tolerance.","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"133 ","pages":"104879"},"PeriodicalIF":4.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined detection of foodborne pathogens in irrigation water in Jiangsu, China. 江苏省灌溉水中食源性致病菌联合检测

IF 4.6 1区农林科学

Food microbiology Pub Date : 2026-01-01 Epub Date: 2025-07-28 DOI: 10.1016/j.fm.2025.104885

Yajing Xie, Jiayao Lu, Bin Li, Dandi Li, Xuefei Du, Bochao Fan, Cunzheng Zhang, Xianjin Liu, Stefan Schmidt

{"title":"Combined detection of foodborne pathogens in irrigation water in Jiangsu, China.","authors":"Yajing Xie, Jiayao Lu, Bin Li, Dandi Li, Xuefei Du, Bochao Fan, Cunzheng Zhang, Xianjin Liu, Stefan Schmidt","doi":"10.1016/j.fm.2025.104885","DOIUrl":"https://doi.org/10.1016/j.fm.2025.104885","url":null,"abstract":"Numerous foodborne disease outbreaks have been attributed to the consumption of fresh produce contaminated with foodborne pathogens. Contaminated irrigation water is a well-established source of bacterial and viral contamination during primary production and is frequently responsible for the contamination of fresh produce. However, efficient methods for the simultaneous detection of bacterial and viral pathogens present in irrigation water are still scarce. A new recombined method was developed to recover two foodborne viruses (human norovirus [huNoV, GI and GII] and rotavirus [RV]) and three pathogenic bacteria (Salmonella Enteritidis, Listeria monocytogenes, and Staphylococcus aureus) from irrigation water for strawberry production. The foodborne viruses and pathogenic bacteria were effectively recovered from spiked water using this method, even at low concentrations. The detection limits of the viruses (huNoV GII, huNoV GI, and RV) were 11, 4.5, and 16 genocopies/mL, while the detection limits of pathogenic bacteria (S. Enteritidis, L. monocytogenes, and S. aureus) were 7, 10, and 4 cells/mL. Using this method, the presence of foodborne pathogens in strawberry irrigation water samples collected from the Jiangsu province (China) was confirmed, with 61 % of samples testing positive for huNoV GII, 38.7 % for huNoV GI, 29.0 % for Salmonella spp., 16.1 % for L. monocytogenes, 9.7 % for S. aureus, and 3.2 % for RV or hepatitis A virus (HAV). HuNoV was also detected in strawberry samples, collected simultaneously from the same farm, when high huNoV detection frequencies and contamination levels were found in irrigation water samples, indicating that huNoV-contaminated irrigation water is a risk when used for strawberry production. This is the first report on the simultaneous detection of selected viral and bacterial pathogens from irrigation water in China, with the new method reported applicable for monitoring other relevant pathogens (e.g., coronavirus) in various water resources.","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"133 ","pages":"104885"},"PeriodicalIF":4.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From genes to products: High-efficiency biosynthesis and holistic optimization strategies for monounsaturated fatty acids. 从基因到产品：单不饱和脂肪酸的高效生物合成和整体优化策略。

IF 4.6 1区农林科学

Food microbiology Pub Date : 2026-01-01 Epub Date: 2025-08-05 DOI: 10.1016/j.fm.2025.104894

Lu-Wei Xu, Yan-Cheng Lin, Yi-Ting Shen, Xin Qi, Zi-Xu Zhang, Wang Ma, Xiao-Man Sun

{"title":"From genes to products: High-efficiency biosynthesis and holistic optimization strategies for monounsaturated fatty acids.","authors":"Lu-Wei Xu, Yan-Cheng Lin, Yi-Ting Shen, Xin Qi, Zi-Xu Zhang, Wang Ma, Xiao-Man Sun","doi":"10.1016/j.fm.2025.104894","DOIUrl":"https://doi.org/10.1016/j.fm.2025.104894","url":null,"abstract":"Monounsaturated fatty acids (MUFAs), particularly palmitoleic, oleic and nervonic acids, serve essential functions in cardiovascular health, metabolic regulation and neuroprotection. Microbial fermentation has emerged as a sustainable production platform that circumvents the geographical constraints and high costs associated with traditional agricultural systems. This review establishes a holistic framework for sustainable MUFA production, systematically integrating upstream metabolic engineering (Δ9 desaturase pathway optimization and chassis strain design), midstream precision fermentation (artificial intelligence-driven dynamic control of bioreactor parameters), and downstream processing (supercritical fluid extraction and microencapsulation). Key strategies include enhancing precursor flux via enzyme engineering, resolving NADPH cofactor imbalances, implementing artificial intelligence-guided genome-scale metabolic models for real-time bioprocess optimization. This review emphasizes the value of microbial production of MUFAs and its optimization methods, while exploring its potential in nutraceutical and biomedical applications.","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"133 ","pages":"104894"},"PeriodicalIF":4.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ex situ conservation on commensal bacteria of crocodile lizard and conservation implications. 迁地保护对鳄鱼蜥蜴共生细菌的影响及其保护意义。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-02-10 DOI: 10.1080/01652176.2025.2463704

Haiying Jiang, Mei Lv, Tengfei He, Mujiao Xie, Zhiwen Zhao, Jiasong He, Shuyi Luo, Yide Guo, Jinping Chen

{"title":"Effects of ex situ conservation on commensal bacteria of crocodile lizard and conservation implications.","authors":"Haiying Jiang, Mei Lv, Tengfei He, Mujiao Xie, Zhiwen Zhao, Jiasong He, Shuyi Luo, Yide Guo, Jinping Chen","doi":"10.1080/01652176.2025.2463704","DOIUrl":"10.1080/01652176.2025.2463704","url":null,"abstract":"Ex situ conservation is an important wildlife conservation strategy, but endangered wildlife in captivity often exhibit high disease rates. Commensal microorganisms are vital for homeostasis, immunity, and linked to diseases. This study analyzed the structure, assembly, variations of the symbiotic microbiota of the endangered crocodile lizard, and their relationship with environment, as well as the effects of captivity on them, to explore why captive reptiles face high dermatosis rates. Results showed that the reptile's microbiota significantly differ from that of its habitat, demonstrating niche specificity. While species richness among organs showed no significant differences, microbial diversity varied considerably. Skin microbiota showed no site-specific clustering. The assembly of skin, oral, and intestinal bacterial communities was dominated by homogeneous selection. The gut and oral bacterial networks were resilient to disturbances, while the skin bacterial network was sensitive. Captivity primarily affected the skin microbiota, reducing its diversity and stability, thereby increasing disease risk, and these effects were not solely attributable to environmental changes. These findings suggested that skin microbial changes in captive reptiles may be responsible for their increased susceptibility to dermatosis in ex situ conservation. This study underscored the importance of understanding reptile-associated microbes for effective conservation strategies and offers potential solutions.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-14"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11816626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between the CUBN and the MIA3 gene copy number variation and growth traits in different cattle breeds. 不同牛品种CUBN与MIA3基因拷贝数变异及生长性状的关系

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-28 DOI: 10.1080/10495398.2025.2450355

Yue Han, Jiwei Liu, Congcong Zhang, Ming Sun, Xuanyu Li, Hongliang Liu, Shengnan Li, Yongchao Zhu, Ruidong Li, Xiaotong Luo, Yumin Zhao, Jian Wu

{"title":"Relationship between the CUBN and the MIA3 gene copy number variation and growth traits in different cattle breeds.","authors":"Yue Han, Jiwei Liu, Congcong Zhang, Ming Sun, Xuanyu Li, Hongliang Liu, Shengnan Li, Yongchao Zhu, Ruidong Li, Xiaotong Luo, Yumin Zhao, Jian Wu","doi":"10.1080/10495398.2025.2450355","DOIUrl":"10.1080/10495398.2025.2450355","url":null,"abstract":"Copy number variations (CNV) are important genetic variations. The endogenous factors cobalamin receptor (CUBN) and MIA SH3 domain ER-derived factor 3 (MIA3) are associated with bone/muscle development and intramuscular fat deposition. There have been no reports on the effects of CUBN and MIA3 CNVs on growth traits of Chinese cattle. This study aimed to determine the correlation between the CUBN and MIA3 CNVs and growth traits in Chinese cattle. qRT-PCR was used to detect the distribution of CUBN and MIA3 CNV and the expression levels of their mRNA, and correlation analysis was conducted between CNV and growth traits. The CUBN was differentially expressed in different breeds of cattle, and CUBN CNV correlated significantly with body height, hip height, body slanting length, and hip width of Grassland Red cattle (CYH); eye muscle area of Yanbian cattle (YB) and Yan Yellow cattle (YH). MIA3 showed no CNV in CYH and YB cattle, and only one deletion type occurred in YH cattle. CUBN and MIA3 mRNA have different expression patterns in different cattle breeds and tissues. In conclusion, CUBN CNV is correlated significantly with growth traits in Chinese cattle and is a novel molecular marker that could be exploited in cattle breeding.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450355"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep. INSL3和WNT2B基因多态性对新疆齐拉黑羊、哈萨克羊和多朗羊季节繁殖性状的影响

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-03 DOI: 10.1080/10495398.2025.2498677

Jingdong Bi, Sulaiman Yiming, Jiaqi Li, Quanfeng Wang, Manjun Zhai, Shaoqi Cao, Mengting Zhu, Hua Yang

{"title":"Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep.","authors":"Jingdong Bi, Sulaiman Yiming, Jiaqi Li, Quanfeng Wang, Manjun Zhai, Shaoqi Cao, Mengting Zhu, Hua Yang","doi":"10.1080/10495398.2025.2498677","DOIUrl":"https://doi.org/10.1080/10495398.2025.2498677","url":null,"abstract":"The purpose of this study was to investigate the polymorphism and genetic correlation of INSL3 and WNT2B genes with seasonal estrus and litter size in three different Xinjiang sheep breeds. The genetic diversity of INSL3 and WNT2B genes were analyzed, and their association with litter size and estrous traits were analyzed. The results showed that two SNPs (SNP1, SNP2) were detected in INSL3 gene and there were three genotypes in SNP2 (INSL3 (A100T)), named of AA, AT and TT, A was the dominant allele. Additionally, five SNPs (SNP3, SNP4, SNP5, SNP6, SNP7) were detected in the WNT2B gene and there were three genotypes in SNP4 (WNT2B (G126T)), named GG, GT and TT, G was dominant allele. SNP2 was in Hardy-Weinberg equilibrium in three sheep breeds (P > 0.05). SNP4 was deviated from Hardy-Weinberg equilibrium in three sheep breeds (P < 0.05). Further, AT genotype of SNP2 (INSL3 (A100T)) could significantly affect the estrus trait in Duolang sheep and Qira black sheep, and related to the litter size in Duolang sheep. The WNT2B significantly affected the estrus and litter size of Duolang sheep and Qira black sheep. INSL3 (A100T) and WNT2B (G126T) may be potential molecular markers for controlling seasonal reproductive trait in sheep.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2498677"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome wide landscaping of copy number variations for horse inter-breed variability. 马种间变异拷贝数变异的全基因组景观分析。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-10 DOI: 10.1080/10495398.2024.2446251

Nitesh Kumar Sharma, Prashant Singh, Bibek Saha, Anuradha Bhardwaj, Mir Asif Iquebal, Yash Pal, Varij Nayan, Sarika Jaiswal, Shiv Kumar Giri, Ram Avatar Legha, T K Bhattacharya, Dinesh Kumar, Anil Rai

{"title":"Genome wide landscaping of copy number variations for horse inter-breed variability.","authors":"Nitesh Kumar Sharma, Prashant Singh, Bibek Saha, Anuradha Bhardwaj, Mir Asif Iquebal, Yash Pal, Varij Nayan, Sarika Jaiswal, Shiv Kumar Giri, Ram Avatar Legha, T K Bhattacharya, Dinesh Kumar, Anil Rai","doi":"10.1080/10495398.2024.2446251","DOIUrl":"https://doi.org/10.1080/10495398.2024.2446251","url":null,"abstract":"Copy number variations (CNVs) have become widely acknowledged as a significant source of genomic variability and phenotypic variance. To understand the genetic variants in horses, CNVs from six Indian horse breeds, namely, Manipuri, Zanskari, Bhutia, Spiti, Kathiawari and Marwari were discovered using Axiom™ Equine Genotyping Array. These breeds differed in agro-climatic adaptation with distinct phenotypic characters. A total of 2668 autosomal CNVs and 381 CNV regions (CNVRs) were identified with PennCNV tool. DeepCNV was employed to re-validate to get 883 autosomal CNVs, of which 9.06% were singleton type. A total of 180 CNVRs were identified after DeepCNV filtering with the estimated length of 3.12 Kb-4.90 Mb. The functional analysis showed the majority of the CNVRs genes enriched for sensory perception and olfactory receptor activity. An Equine CNVs database, EqCNVdb (http://backlin.cabgrid.res.in/eqcnvdb/) was developed which catalogues detailed information on the horse CNVs, CNVRs and gene content within CNVRs. Also, three random CNVRs were validated with real-time polymerase chain reaction. These findings will aid in the understanding the horse genome and serve as a preliminary foundation for future CNV association research with commercially significant equine traits. The identification of CNVs and CNVRs would lead to better insights into genetic basis of important traits.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2446251"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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