IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-01-04 DOI: 10.1080/01652176.2023.2298089

B Agüero, F Berrios, C Pardo-Roa, N Ariyama, B Bennett, R A Medina, V Neira

{"title":"First detection of Omicron variant BA.4.1 lineage in dogs, Chile.","authors":"B Agüero, F Berrios, C Pardo-Roa, N Ariyama, B Bennett, R A Medina, V Neira","doi":"10.1080/01652176.2023.2298089","DOIUrl":"10.1080/01652176.2023.2298089","url":null,"abstract":"SARS-CoV-2's rapid global spread caused the declaration of COVID-19 as a pandemic in March 2020. Alongside humans, domestic dogs and cats are also susceptible to infection. However, limited reports on pet infections in Chile prompted a comprehensive study to address this knowledge gap. Between March 2021 and March 2023, the study assessed 65 pets (26 dogs and 39 cats) from 33 COVID-19+ households alongside 700 nasal swabs from animals in households with unknown COVID-19 status. Using RT-PCR, nasal, fecal, and environmental samples were analyzed for the virus. In COVID-19+ households, 6.06% tested positive for SARS-CoV-2, belonging to 3 dogs, indicating human-to-pet transmission. Pets from households with unknown COVID-19 status tested negative for the virus. We obtained 2 SARS-CoV-2 genomes from animals, that belonged to Omicron BA.4.1 variant, marking the first report of pets infected with this lineage globally. Phylogenetic analysis showed these sequences clustered with human sequences collected in Chile during the same period when the BA.4.1 variant was prevalent in the country. The prevalence of SARS-CoV-2 in Chilean pets was relatively low, likely due to the country's high human vaccination rate. Our study highlights the importance of upholding and strengthening human vaccination strategies to mitigate the risk of interspecies transmission. It underscores the critical role of the One Health approach in addressing emerging zoonotic diseases, calling for further research on infection dynamics and risk factors for a comprehensive understanding.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10769545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139089342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the clinical usefulness of pancreatic alpha amylase as a novel biomarker in dogs with acute pancreatitis: a pilot study. 评估胰腺α-淀粉酶作为一种新型生物标记物在犬急性胰腺炎患者中的临床实用性：一项试点研究。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-03-18 DOI: 10.1080/01652176.2024.2326007

Keon Kim, Hee-Hong Kim, Jae-Beom Joo, Ock-Kyu Kim, Sin-Wook Park, Guk-Hyun Suh, Woong-Bin Ro, Chang-Min Lee

{"title":"Evaluation of the clinical usefulness of pancreatic alpha amylase as a novel biomarker in dogs with acute pancreatitis: a pilot study.","authors":"Keon Kim, Hee-Hong Kim, Jae-Beom Joo, Ock-Kyu Kim, Sin-Wook Park, Guk-Hyun Suh, Woong-Bin Ro, Chang-Min Lee","doi":"10.1080/01652176.2024.2326007","DOIUrl":"10.1080/01652176.2024.2326007","url":null,"abstract":"Pancreatic alpha amylase (P-AMY) is used as a biomarker of acute pancreatitis (AP) in human medicine. To our knowledge, there are no studies evaluating the usefulness of P-AMY in dogs with AP. In this study, we evaluated the diagnostic value of P-AMY, currently not verified in veterinary medicine. The AP group (n = 40) consisted of dogs with AP diagnosed using clinical signs and laboratory examinations, including abnormal canine pancreatic lipase (cPL) concentration, and compatible abdominal ultrasound examination at first presentation. Evaluation of the canine AP severity (CAPS) score was performed. The control group (n = 38) was composed of normal dogs without any abnormalities in clinical findings, blood exams or diagnostic imaging. The correlation of P-AMY with cPL was confirmed by Pearson's correlation analysis (r = 0.564, p < .001). The sensitivity and specificity for the most appropriate cut-off values of P-AMY were recorded similar to the values of DGGR. The dogs with AP and CAPS ≥11 had significantly higher serum P-AMY (p = .016) contrary to DGGR lipase and cPL. Furthermore, there was a significant difference in the median P-AMY dependent on the presence of systemic inflammatory response syndrome (p = .001). P-AMY showed similar level of diagnostic accuracy along with sensitivity and specificity compared to DGGR lipase. In addition, P-AMY showed a significant association with CAPS score, contrary to cPL and DGGR lipase. Along with other biomarkers associated with AP, P-AMY has the potential of usefulness as a supportive diagnostic and prognostic biomarker of AP in dogs.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10949834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Live performance, nutrient digestibility, immune response and fecal microbial load modulation in Japanese quails fed a Bacillus-based probiotic alone or combination with xylanase. 单独或与木聚糖酶联合使用芽孢杆菌益生菌喂养日本鹌鹑的活体表现、营养消化率、免疫反应和粪便微生物负荷调节。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-06-21 DOI: 10.1080/01652176.2024.2364641

Asad Sultan, Syed Murtaza, Shabana Naz, Ziaul Islam, Abdulwahed Fahad Alrefaei, Rifat Ullah Khan, Samia H Abdelrahman, A Chandrasekaran

{"title":"Live performance, nutrient digestibility, immune response and fecal microbial load modulation in Japanese quails fed a Bacillus-based probiotic alone or combination with xylanase.","authors":"Asad Sultan, Syed Murtaza, Shabana Naz, Ziaul Islam, Abdulwahed Fahad Alrefaei, Rifat Ullah Khan, Samia H Abdelrahman, A Chandrasekaran","doi":"10.1080/01652176.2024.2364641","DOIUrl":"10.1080/01652176.2024.2364641","url":null,"abstract":"Animal industry seeks cost-effective solutions to enhance performance and health of domestic animals. This study investigated the effects of supplementing Bacillus spp. probiotics and xylanase on 2000 one-day-old Japanese quails, randomly assigned to four treatment groups (10 replicates). The control group received no supplementation, while the others were supplemented with a Bacillus-based probiotic at 7.5 × 107 cfu/kg of feed, xylanase enzyme (2,000 U/kg) alone or in combination. Quails receiving both probiotic and enzyme exhibited significantly (p < 0.01) higher weekly and overall weight gain, and lower feed conversion ratios compared to the control group. Dressing percentage was higher (p < 0.01), and mortality lower in birds supplemented with a combination of enzyme and probiotic. Antibody titres against infectious bronchitis and infectious bursal disease were significantly (p < 0.01) higher in quails receiving combined probiotic and enzyme supplementation, while titres against Newcastle disease virus were higher (p < 0.01) in groups supplemented with probiotic and enzyme individually or in combination. Additionally, digestibility was significantly (p < 0.01) higher in groups receiving combined enzyme and probiotic supplementation, with higher apparent metabolizable energy compared to the control. The populations of beneficial Lactobacillus increased, while harmful E. coli and Salmonella decreased significantly in quails supplemented with both probiotic and enzyme. In conclusion, supplementing xylanase enzyme and probiotic together in Japanese quails positively influenced growth, nutrient digestibility, immune response, and cecal microbiota.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11195456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial resistance and virulence profiling of Staphylococcus pseudintermedius isolated from cats, Bangladesh. 从孟加拉国猫体内分离出的假中间葡萄球菌的抗菌药耐药性和毒力特征。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-03-15 DOI: 10.1080/01652176.2024.2326848

Eaftekhar Ahmed Rana, Tanvir Ahmad Nizami, Md Sayedul Islam, Subrata Sarker, Hafizar Rahman, Azizul Hoque, Mizanur Rahman

{"title":"Antimicrobial resistance and virulence profiling of Staphylococcus pseudintermedius isolated from cats, Bangladesh.","authors":"Eaftekhar Ahmed Rana, Tanvir Ahmad Nizami, Md Sayedul Islam, Subrata Sarker, Hafizar Rahman, Azizul Hoque, Mizanur Rahman","doi":"10.1080/01652176.2024.2326848","DOIUrl":"10.1080/01652176.2024.2326848","url":null,"abstract":"Staphylococcus pseudintermedius is a significant bacterial pathogen that frequently colonizes different body sites and mucous membranes of pets. The objectives of the cross-sectional study were to estimate the prevalence, antimicrobial resistance pattern, and detection of diverse resistance as well as virulence genes of S. pseudintermedius in cats. A standard bacteriological method, species-specific gene and different antimicrobial resistance as well as virulence genes were confirmed by PCR assay. A total of 233 swab samples were collected from different body sites of 102 cats, among them 146 swabs from 73 healthy cats, and 87 from 29 diseased cats. Overall, prevalence of S. pseudintermedius in cats was 12.01%, while dermatitis and otitis affected cats were 26.08% and 33.33%, respectively. The highest antimicrobial resistance was observed against penicillin (96.42%) followed by streptomycin (85.71%) and erythromycin (78.57%). Moreover, 89.28% of S. pseudintermedius isolates exhibit multi-drug resistance (MDR) (≥ 3 classes' antimicrobial resistant). In addition, 17.86% isolates harbored the mecA gene; thus, were classified as methicillin-resistant S. pseudintermedius (MRSP). Furthermore, the erythromycin resistance genes ermA and ermB were harbored by 25% and 10.71% of isolates, while 42.86% and 17.86% of isolates carried tetK and tetL (tetracycline resistance) genes, respectively. In virulence profiling, 32.14% (sea) and 10.71% (seb) of isolates were found positive for enterotoxin genes, whereas, the toxic shock syndrome toxin-1 (tst-1) gene and the Panton-Valentine leukocidin gene (pvl) were detected in 25% and 14.29% of isolates, respectively. To our knowledge, this is the first report of cats in Bangladesh for MDR S. pseudintermedius, MRSP, and their virulence profiling.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10946260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140133198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and evaluation of a chicken embryo fibroblast cell culture based live attenuated Indian strain duck plague vaccine. 基于鸡胚成纤维细胞培养的印度鸭瘟减毒活疫苗的开发与评估。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-05-10 DOI: 10.1080/01652176.2024.2350668

Satyabrata Dandapat, Suresh Bindu, Gaurav Kumar Sharma, Sivasankar Panickan, Sukdeb Nandi, G Saikumar, Kuldeep Dhama

{"title":"Development and evaluation of a chicken embryo fibroblast cell culture based live attenuated Indian strain duck plague vaccine.","authors":"Satyabrata Dandapat, Suresh Bindu, Gaurav Kumar Sharma, Sivasankar Panickan, Sukdeb Nandi, G Saikumar, Kuldeep Dhama","doi":"10.1080/01652176.2024.2350668","DOIUrl":"10.1080/01652176.2024.2350668","url":null,"abstract":"Duck plague (DP) is an acute, contagious and fatal disease, caused by duck enteritis virus (DEV), with worldwide distribution causing several outbreaks and posing severe economic losses. The present study was carried out with a goal of development of a live attenuated cell culture based DP vaccine using an Indian strain of DEV and evaluation of its safety, efficacy along with complete genome analysis. The live attenuated DP vaccine (DPvac/IVRI-19) was developed by serial propagation of a virulent isolate of DEV (DEV/India/IVRI-2016) in the chicken embryo fibroblast (CEF) primary cell culture. Adaptation of DEV in CEF cell culture was indicated by more rapid appearance of cytopathic effects (CPE) and gradual increase of virus titre, which reached up to 107.5 TCID50/mL after 41 passages. The safety, immunogenicity and efficacy of the vaccine were determined by immunization trials in ducklings. The DPvac/IVRI-19 was found to be avirulent and completely safe in the ducklings. Further, the vaccine induced both humoral and cell mediated immune responses and afforded 100% protection against the virulent DEV challenge. A comparison of the whole genome of DPvac/IVRI-19 (MZ911871) and DEV/India/IVRI-2016 (MZ824102) revealed significant number of mutations, which might be associated with viral attenuation. Phylogenetic tree of DEV/India/IVRI-2016 revealed its evolutionary relationship with other DEV isolates, but it formed a separate cluster with certain unique mutations. Thus, with the proven safety and 100% efficacy, the DPvac/IVRI-19 is suitable for large scale production with precisely pure form of vaccine and has potential utility at national and global levels.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11089916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus. 暴露于低毒性非洲猪瘟病毒分离株的野猪的三级淋巴器官。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-03-27 DOI: 10.1080/01652176.2024.2331525

Néstor Porras, José M Sánchez-Vizcaíno, Antonio Rodríguez-Bertos, Aleksandra Kosowska, José Á Barasona

{"title":"Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus.","authors":"Néstor Porras, José M Sánchez-Vizcaíno, Antonio Rodríguez-Bertos, Aleksandra Kosowska, José Á Barasona","doi":"10.1080/01652176.2024.2331525","DOIUrl":"10.1080/01652176.2024.2331525","url":null,"abstract":"Despite the great interest in the development of a vaccine against African swine fever (ASF) in wild boar, the immunological mechanisms that induce animal protection are still unknown. For this purpose, tertiary lymphoid organs (TLOs) of wild boar were characterised and compared with mucosa-associated lymphoid tissues (MALTs) by histopathology, histomorphometry and immunohistochemistry (CD3, CD79, PAX5, LYVE1, fibronectin). In addition, real-time polymerase chain reaction (qPCR) and immunohistochemistry (p72) were used to evaluate the presence of ASF virus (ASFV) in blood and tissues samples, respectively. TLOs were observed in animals infected with a low-virulent ASFV isolate (LVI), animals co-infected with low and high-virulent ASFV isolates (LVI-HVI) and animals infected only with the high virulence isolate (HVI). TLOs in LVI and LVI-HVI groups were located adjacent to the mucosa and presented a similar structure to MALT. Immunoexpresion of p72 observed in the inflammatory cells adjacent to TLOs/MALTs confirmed its development and reactivity generated by ASF attenuated isolates. Immunohistochemical evaluation, based on cellular composition (T and B lymphocytes), and histomorphometrical study revealed a more pronounced maturation of TLOs/MALTs in the LVI-HVI group. It is currently unclear whether these formations play a protective role by contributing to local immunity in chronic inflammatory diseases. However, the structural similarities between TLOs and MALTs and the location of TLOs close to the mucosa suggest that they may perform a similar function, facilitating a local protective response. Nevertheless, further investigations are warranted to assess the cellular and humoral dynamics of these lymphoid organs induced by attenuated isolates.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasitic findings on threatened pudu deer from Central Chile accounts first genetic characterization of lice parasitizing P. puda in Chile and the first molecular report of Taenia hydatigena metacestodes in this species. 对智利中部濒临灭绝的普渡鹿的寄生虫研究结果首次说明了寄生于智利普渡鹿的虱子的遗传特征，并首次从分子角度报告了该物种中的水蚤元绦虫。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-01-19 DOI: 10.1080/01652176.2024.2302027

Tamara Muñoz-Caro, María Fernanda González, Rodrigo Villalobos, Alejandro Hidalgo

{"title":"Parasitic findings on threatened pudu deer from Central Chile accounts first genetic characterization of lice parasitizing P. puda in Chile and the first molecular report of Taenia hydatigena metacestodes in this species.","authors":"Tamara Muñoz-Caro, María Fernanda González, Rodrigo Villalobos, Alejandro Hidalgo","doi":"10.1080/01652176.2024.2302027","DOIUrl":"10.1080/01652176.2024.2302027","url":null,"abstract":"Southern pudu (Pudu puda) is a threatened endemic deer of the temperate forests of Chile. In recent years pudu populations rates have decreased mainly due to anthropogenic causes including forest loss and landscape fragmentation. In this context, the parasitic fauna of Chilean pudu has been scarcely investigated. The aim of this study was to determine the parasitic status of rescued pudu n = 13 from its natural habitat in Central Chile (Maule region) during March 2022 and June 2023 by applying morphological, histopathological, and molecular analyses. As result, we report the presence of transmission of parasites from dogs to pudus as showed by the presence of metacestodes of the parasite Taenia hydatigena on omentum, liver, and pleura of pudus during postmortem examinations, being the first molecular report on the presence of this parasite on Chilean pudu. Meanwhile, ectoparasite examinations determined the presence of chewing and sucking lice on pudu exemplars here analysed. Molecular and phylogenetic analysis of lice revealed new insights on Bovicola and Anoplura lice parasitizing P. puda in Chile, equally being the first genetic characterization of lice parasitizing pudu exemplars in Chile. In addition, parasite loads of lice and metacestodes were analysed. However, no statistically significance was observed when comparing environmental and individual traits influence on parasite load variation. Overall, the study area is the northern limit of habitat distribution of this specie in Chile and we here provide novel information on pudu deer parasites, thus making a useful and valuable contribution to the parasitological knowledge on this threatened species.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10802800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139503169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sleep disturbance in clinical and preclinical scrapie-infected sheep measured by polysomnography. 通过多导睡眠图测量临床和临床前瘙痒症感染绵羊的睡眠障碍。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-05-02 DOI: 10.1080/01652176.2024.2349674

Diego Sola Fraca, Ernesto Sánchez Garrigós, Jorge de Francisco Moure, Belén Marín Gonzalez, Juan José Badiola Díez, Cristina Acín Tresaco

{"title":"Sleep disturbance in clinical and preclinical scrapie-infected sheep measured by polysomnography.","authors":"Diego Sola Fraca, Ernesto Sánchez Garrigós, Jorge de Francisco Moure, Belén Marín Gonzalez, Juan José Badiola Díez, Cristina Acín Tresaco","doi":"10.1080/01652176.2024.2349674","DOIUrl":"10.1080/01652176.2024.2349674","url":null,"abstract":"Neurodegenerative diseases are characterised by neuronal loss and abnormal deposition of pathological proteins in the nervous system. Among the most common neurodegenerative diseases are Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease and transmissible spongiform encephalopathies (TSEs). Sleep and circadian rhythm disturbances are one of the most common symptoms in patients with neurodegenerative diseases. Currently, one of the main objectives in the study of TSEs is to try to establish an early diagnosis, as clinical signs do not appear until the damage to the central nervous system is very advanced, which prevents any therapeutic approach. In this paper, we provide the first description of sleep disturbance caused by classical scrapie in clinical and preclinical sheep using polysomnography compared to healthy controls. Fifteen sheep classified into three groups, clinical, preclinical and negative control, were analysed. The results show a decrease in total sleep time as the disease progresses, with significant changes between control, clinical and pre-clinical animals. The results also show an increase in sleep fragmentation in clinical animals compared to preclinical and control animals. In addition, sheep with clinical scrapie show a total loss of Rapid Eye Movement sleep (REM) and alterations in Non Rapid Eyes Movement sleep (NREM) compared to control sheep, demonstrating more shallow sleep. Although further research is needed, these results suggest that prion diseases also produce sleep disturbances in animals and that polysomnography could be a diagnostic tool of interest in clinical and preclinical cases of prion diseases.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11073408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete coding region sequence analyses and antigenic characterization of emerging lineage G-IX of foot- and-mouth disease virus serotype Asia1. 新出现的亚洲 1 号口蹄疫病毒血清型 G-IX 系的完整编码区序列分析和抗原特征。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-06-21 DOI: 10.1080/01652176.2024.2367215

Manoranjan Rout, Shyam Singh Dahiya, Saravanan Subramaniam, Ramakant Acharya, Reshama Samanta, Jitendra Kumar Biswal, Jajati Keshari Mohapatra, Rabindra Prasad Singh

{"title":"Complete coding region sequence analyses and antigenic characterization of emerging lineage G-IX of foot- and-mouth disease virus serotype Asia1.","authors":"Manoranjan Rout, Shyam Singh Dahiya, Saravanan Subramaniam, Ramakant Acharya, Reshama Samanta, Jitendra Kumar Biswal, Jajati Keshari Mohapatra, Rabindra Prasad Singh","doi":"10.1080/01652176.2024.2367215","DOIUrl":"10.1080/01652176.2024.2367215","url":null,"abstract":"Foot-and-mouth disease Virus (FMDV) serotype Asia1 is prevalent in the Indian subcontinent, with only G-III and G-VIII reported in India until 2020. However, in 2019, a novel genetic group within serotype Asia1, designated as G-IX, emerged in Bangladesh, followed by its detection in India in 2020. This report presents analyses of the complete coding region sequences of the G-IX lineage isolates. The length of the open reading frame (ORF) of the two G-IX isolates was 6990 nucleotides without any deletion or insertion. The G-IX isolates showed the highest sequence similarity with an isolate of G-III at the ORF, L, P2, and P3 regions, and with an isolate of G-VIII at the P1 region. Phylogenetic analysis based on the capsid region (P1) supports the hypothesis that G-VIII and G-IX originated from a common ancestor, as speculated earlier. Further, VP1 region-based phylogenetic analyses revealed the re-emergence of G-VIII after a gap of 3 years. One isolate of G-VIII collected during 2023 revealed a codon insertion in the G-H loop of VP1. The vaccine matching studies support the suitability of the currently used Indian vaccine strain IND63/1972 to contain outbreaks due to viruses belonging to G-IX.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11195457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial susceptibility and resistome of Actinobacillus pleuropneumoniae in Taiwan: a next-generation sequencing analysis. 台湾胸膜肺炎放线杆菌的抗菌药敏感性和耐药性组：新一代测序分析。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-04-30 DOI: 10.1080/01652176.2024.2335947

Chiao-Hsu Ke, Pan-Yun Lai, Feng-Yang Hsu, Po-Ren Hsueh, Ming-Tang Chiou, Chao-Nan Lin

{"title":"Antimicrobial susceptibility and resistome of Actinobacillus pleuropneumoniae in Taiwan: a next-generation sequencing analysis.","authors":"Chiao-Hsu Ke, Pan-Yun Lai, Feng-Yang Hsu, Po-Ren Hsueh, Ming-Tang Chiou, Chao-Nan Lin","doi":"10.1080/01652176.2024.2335947","DOIUrl":"https://doi.org/10.1080/01652176.2024.2335947","url":null,"abstract":"Actinobacillus pleuropneumoniae infection causes a high mortality rate in porcine animals. Antimicrobial resistance poses global threats to public health. The current study aimed to determine the antimicrobial susceptibilities and probe the resistome of A. pleuropneumoniae in Taiwan. Herein, 133 isolates were retrospectively collected; upon initial screening, 38 samples were subjected to next-generation sequencing (NGS). Over the period 2017-2022, the lowest frequencies of resistant isolates were found for ceftiofur, cephalexin, cephalothin, and enrofloxacin, while the highest frequencies of resistant isolates were found for oxytetracycline, streptomycin, doxycycline, ampicillin, amoxicillin, kanamycin, and florfenicol. Furthermore, most isolates (71.4%) showed multiple drug resistance. NGS-based resistome analysis revealed aminoglycoside- and tetracycline-related genes at the highest prevalence, followed by genes related to beta-lactam, sulfamethoxazole, florphenicol, and macrolide. A plasmid replicon (repUS47) and insertion sequences (IS10R and ISVAp11) were identified in resistant isolates. Notably, the multiple resistance roles of the insertion sequence IS10R were widely proposed in human medicine; however, this is the first time IS10R has been reported in veterinary medicine. Concordance analysis revealed a high consistency of phenotypic and genotypic susceptibility to florphenicol, tilmicosin, doxycycline, and oxytetracycline. The current study reports the antimicrobial characterization of A. pleuropneumoniae for the first time in Taiwan using NGS.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11064736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140871640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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