农林科学最新文献

{"title":"Multiplexed immunoassay for a serum autoantibody biomarker panel in diagnostic and prognostic prediction of canine mammary tumors.","authors":"Chih-Ching Wu, Chia-Yu Chang, Pei-Yi Chou, Xiu-Ya Chan, Chun-Chueh Huang, Youngsen Yang, Hao-Ping Liu","doi":"10.1080/01652176.2024.2435978","DOIUrl":"10.1080/01652176.2024.2435978","url":null,"abstract":"<p><p>Canine mammary tumor (CMT) is a prevalent and destructive disease often diagnosed at an advanced stage, leading to poor outcomes. Currently, there is a lack of effective biomarkers for early detection and prognostic prediction of CMT. To improve CMT detection, we established a multiplexed immunoassay using a fluorescence bead-based suspension array system to measure serum levels of autoantibodies against four CMT-associated proteins (AGR2, HAPLN1, IGFBP5, and TYMS) in CMT patients. Our data revealed that serum levels of the four autoantibodies (anti-AGR2, anti-HAPLN1, anti-IGFBP5, and anti-TYMS) were significantly elevated in CMT patients (<i>n</i> = 158) compared to healthy individuals (<i>n</i> = 39). Notably, serum levels of anti-AGR2, anti-HAPLN1, and anti-TYMS in the dogs with stage I CMT (<i>n</i> = 56) were higher than those in the healthy group. Using a marker panel consisting of the four autoantibodies for detecting malignant CMT (<i>n</i> = 125) achieved a sensitivity of 50.4% and a specificity of 90%. Furthermore, higher levels of anti-AGR2, anti-HAPLN1, anti-IGFBP5, and anti-TYMS were associated with poorer survival in CMT patients. Collectively, we established a multiplexed immunoassay platform to detect serum autoantibodies and demonstrated that a tailored autoantibody marker panel shows potential clinical applicability for the diagnosis and prognosis of CMT.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-12"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of citrinin biosynthesis in Monascus purpureus: Impacts on growth, morphology, and pigments production.","authors":"Xizi Zhang, Wei Chen, Chengtao Wang","doi":"10.1016/j.fm.2024.104698","DOIUrl":"https://doi.org/10.1016/j.fm.2024.104698","url":null,"abstract":"<p><p>Fungal hyphae self-assemble a variety of cellular macrostates, ranging from suspended mycelium to dense pellets, all inextricably linked to their productivity. In this study, using CRISPR/Cas technology, we constructed a ctnA knockout strain (ΔctnA) and an overexpression strain (A2) so as to investigate the effects of interfering with citrinin biosynthesis on the growth, morphology and pigmentation of M.purpureus. Results indicated that deletion of ctnA in M. purpureus RP2 led to increased mycelium length, delayed conidium formation, and a citrinin content of 22% of the wild-type strain. Conversely, ctnA overexpression in strain A2 resulted in delayed mycelial growth, normal conidium formation, and a citrinin content of 120% compared to the wild-type strain, with minimal effects on pigments content. Notably, the ΔctnA strain formed small, tightly structured pellets (mean diameter 1.2 ± 0.06 mm) and exhibited low citrinin content, promoting pigments production. Our findings suggest a complex interplay between citrinin biosynthesis and morphological development, providing insights for optimizing metabolite production in industrial applications.</p>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"127 ","pages":"104698"},"PeriodicalIF":4.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The investigation of the mechanism underlying variations in oxidative stress tolerance of Lacticaseibacillus paracasei resulting from fermentation methods through endogenous CRISPR-Cas9 editing methodology.","authors":"Pengyu Wu, Yutian Zhang, Qiantong Shan, Ziyang Wang, Shuang Cheng, Laiyou Wang, Bingbing Liu, Wenhuan Li, Zhenmin Chen, Jiancheng Luo, Yunxiang Liang","doi":"10.1016/j.fm.2024.104697","DOIUrl":"https://doi.org/10.1016/j.fm.2024.104697","url":null,"abstract":"<p><p>The probiotic effects of lactic acid bacteria make them widely used in human and animal breeding industry. However, the presence of oxidative stress during the production and application process can cause bacterial damage or even death, significantly compromising the functionality of probiotics. Despite its potential for broader application scenarios that could provide a more comprehensive understanding of bacteria's internal adaptation strategies, there is a lack of research investigating oxidative stress from the perspective of culture methods. In this study, the tolerance to oxidative stress was compared between bacteria cultivated through solid-state fermentation (SSF) and liquid-state fermentation (LSF), and the physiological and transcriptional disparities between these two bacterial strains were investigated. Additionally, a novel and efficient gene editing method was developed to elucidate the genetic basis underlying these differences in tolerance. The results demonstrated a significantly higher tolerance to oxidative stress in SSF bacteria compared to LSF bacteria, along with a stronger capacity for maintaining intracellular microenvironment stability and the activity of key metabolic enzymes. It is noteworthy that the bacteria from SSF significantly enhance the transport of carbohydrate substances and facilitate intracellular metabolic flow. Gene editing experiments have confirmed the crucial role of genes glpF and glpO in regulating the glycerol metabolism pathway, which is essential for enhancing the tolerance of bacteria from SSF to oxidative stress. Based on these findings, the mechanism underlying the disparity in oxidative stress tolerance resulting from different culture methods has been summarized. Furthermore, investigation into different culture modes has revealed that moderate oxygen levels during cultivation significantly influence variation in bacterial tolerance to oxidative stress. Importantly, these variations are species-specific and depend on the ecological niche distribution of Lactobacilli. These findings elucidate a novel mechanism by which Lacticaseibacillus paracasei Zhang tolerates oxidative stress, and also suggest that distinct cultivation and processing methods should be tailored based on the specific Lactobacilli groups to achieve optimal application effects in production.</p>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"127 ","pages":"104697"},"PeriodicalIF":4.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction and cross-contamination potential of prepared beef steak isolates Pseudomonas weihenstephanensis and Macrococcus caseolyticus in biofilms of dual-species.","authors":"Wendi Zhang, Yunhao Ma, Yong Xie, Xiaoyan Liu, Lijun Tan, Jinsong Zhao, Yongsheng Ni, Zhaoming Wang, Cong Li, Baocai Xu","doi":"10.1016/j.fm.2024.104685","DOIUrl":"https://doi.org/10.1016/j.fm.2024.104685","url":null,"abstract":"<p><p>This study evaluated the interactions between single or dual-species biofilms formed by dominant spoilage bacteria P. weihenstephanensis and M. caseolyticus isolated from refrigerated, spoilage prepared beef steaks at 4 °C and elucidated the interactive behavior of biofilm development in dual species. In addition, the relationship between biofilm formation capacity and cross-contamination was analyzed by simulating surface to food contact transfer. The results showed that the two species exhibited synergism as biofilms developed, which was the main mode of interaction observed. Under aerobic conditions, Pseudomonas weihenstephanensis and Macrococcus caseolyticus co-cultured for 96 h showed obvious biofilm formation ability, resulting in greater cross-contamination. Scanning electron microscopy and Confocal laser scanning microscopy showed the formation of flattened dense biofilms in the co-culture. The significant increase in Fe content and decrease in siderophore content of the dual-species biofilm as determined by ICP-MS was attributed to respiratory inhibition resulting in a decrease in the transcription of genes regulating the two-component regulatory system of Macrococcus tyrolyticus SrrAB and an increase in the expression of cytoplasmic hydrolase leading to the rupture of the release of hemoglobin to provide a source of iron for P. weihenstephanensis. The increase of heme content in the supernatant of dual-species and the results of RT-qPCR showed that the gene expression of the heme transport system of P. weihenstephanensis was significantly up-regulated and the siderophore gene expression was decreased, which further revealed that P. weihenstephanensis preferentially uses the heme uptake system to take up the iron source provided by M. caseolyticus for P. weihenstephanensis. Overall, our results provide insight into the complex dynamics of biofilms formed by P. weihenstephanensis and M. caseolyticus, emphasizing that the iron reaction pathway may be a key factor influencing the growth of P. weihenstephanensis biofilms, and that these results will provide a theoretical basis for the control of spoilage of refrigerated foods.</p>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"127 ","pages":"104685"},"PeriodicalIF":4.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing wine malolactic fermentation: Variable effect of yeast mannoproteins on Oenococcus oeni strains.","authors":"Paloma Toraño, Alba Martín-García, Albert Bordons, Nicolas Rozès, Cristina Reguant","doi":"10.1016/j.fm.2024.104689","DOIUrl":"https://doi.org/10.1016/j.fm.2024.104689","url":null,"abstract":"<p><p>Lactic acid bacteria (LAB), principally Oenococcus oeni, play crucial roles in wine production, contributing to the transformation of L-malic acid into L-lactic acid during malolactic fermentation (MLF). This fermentation is influenced by different factors, including the initial LAB population and wine stress factors, such as nutrient availability. Yeast mannoproteins can enhance LAB survival in wine. This study explored in model conditions the impact of a commercial mannoprotein extract on MLF dynamics in ten O. oeni strains. The results revealed strain-specific responses in fermentation kinetics and mannoprotein utilization. Mannoprotein addition influenced MLF outcomes, depending on the strain and concentration. The variability in MLF confirmed different technological aptitude of the strains used. The α-mannosidase enzymatic activity was determined and showed higher values in the supernatant than in whole cells. Moreover, α-mannosidase activity varied among strains, suggesting differential regulation in response to fermentation conditions. These findings highlight the importance of understanding mannoprotein interactions with O. oeni for optimizing MLF efficiency and enhancing wine quality. Further research under cellar conditions is needed to evaluate the potential of yeast mannoproteins to promote MLF.</p>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"127 ","pages":"104689"},"PeriodicalIF":4.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity of duck circovirus and novel goose parvovirus co-infection in SPF ducks.","authors":"Yudong Zhu, Qiong Wu, Mian Wu, Dalin He, Bingrong Wu, Mingtian Mao, Wentao Tang, Jiake Li, Caiqi Wang, Hui Zhao, Yafei Qin, Youxiang Diao, Yi Tang","doi":"10.1080/03079457.2024.2383231","DOIUrl":"10.1080/03079457.2024.2383231","url":null,"abstract":"<p><p>Duck circovirus (DuCV) is one of the most prevalent infectious viruses in the duck industry in China. Although the clinical signs vary, it often causes immunosuppression in the host and leads to secondary infection with other pathogens. Novel goose parvovirus (NGPV) mainly infects ducks and causes short beak and dwarfism syndrome in ducks. However, the incidence of infection in ducks has increased in recent years, and the phenomenon of mixed infection with DuCV is common, resulting in more severe clinical morbidity. However, there are no systematic studies evaluating the presence of mixed infections. In order to investigate the synergistic pathogenicity of DuCV and NGPV co-infection in SPF ducks, a comparative experiment using DuCV and NGPV co-infection and mono-infection bird models was established. The results showed that the clinical signs of short beak, dwarfism and immunosuppression were more obvious in DuCV and NGPV co-infected ducks; the tissue damage of target organs was more serious, and the viral titre in organs and cloacal swabs were more significant compared with those of SPF ducks infected with only one virus. The results indicated that co-infection with DuCV and NGPV could promote viral replication and cause more severe tissue damage and immunosuppression than single virus infection. The present study reveals that the co-infection of NGPV and DuCV has a synergistic pathogenic effect from the aspect of pathogenicity, and the conclusions drawn not only clarify the direction of the subsequent research on the mechanism of co-infection of NGPV and DuCV, but also provide a scientific basis for the research on the co-infection of immunosuppressive pathogens and other pathogens.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"76-82"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a recombinant non-replicating Newcastle disease virus.","authors":"Pheik-Sheen Cheow, Tiong Kit Tan, Adelene Ai-Lian Song, Khatijah Yusoff, Suet Lin Chia","doi":"10.1080/03079457.2024.2403412","DOIUrl":"10.1080/03079457.2024.2403412","url":null,"abstract":"<p><strong>Research highlights: </strong>Development of nr-NDV.Reverse transfection was applied for the recovery of nr-NDV.Propagation of nr-NDV was done by sub-passaging transfected BSR T7/5 cells.Safety profile was done to prove that the nr-NDV is non-replicating.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"149-157"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of global warming effects on juvenile rainbow trout: focus on immunohistochemistry and osmoregulation.","authors":"Zehra Alkan, Boran Karataş, Ahmet Sepil","doi":"10.1007/s10695-024-01431-5","DOIUrl":"10.1007/s10695-024-01431-5","url":null,"abstract":"<p><p>The negative effects of global warming also directly affect aquatic populations. Consequences such as evaporation due to chronic temperature increase, increase in salinity, and increase in stock density per unit volume are potential stress factors. While creating the trial design, an attempt was made to simulate the effects of global warming, especially on species living in salty and brackish water biotopes. In this study, changes in the gills of rainbow trout (Oncorhynchus mykiss) acclimated to 0, 20, and 38 ‰ of saline in the laboratory were examined histologically and immunohistochemically and blood serum osmolarity. In addition, the water temperature was changed, and experiments were carried out at 16, 19, and 22 °C for each salinity group in parallel with the increase in salinity. However, to simulate the decrease in water volume and intensive stocking due to the potential impact of climate change, the study was carried out using 15 fishes in low-volume aquariums (45 L). Tap water that had been kept for at least 3 days was used in the aquariums. To protect the water quality, independent aquariums with sponge filters were used, and since the aim was to keep dissolved oxygen low, no ventilation system other than the sponge filter was used. In order to minimize the deterioration in water quality during the trial, a 15% water change was performed by performing a bottom flush every 4 days and water of the same temperature and salinity was added as much as the reduced volume. In addition, since increasing stock density due to temperature increase and water decrease will cause the amount of dissolved oxygen to decrease, pure oxygen was not entered into any tank throughout the experiment, and the concentration was requested to be at a low level (7 ± 0.13 mg/L) in all groups. The trials were terminated at the end of the 71st day. Increased serum osmolarity values were observed due to the increase in salinity, and the highest serum osmolarity value was measured at 644 mOsm/kg in the 38 ‰ salinity group. Differences between the groups were found to be statistically significant (p < 0.05). It was observed that the number of cells containing Na⁺/K⁺-ATPase increased depending on salinity. Also, the number of chloride cells reached the maximum level in the 38 ‰ salinity group. Due to increasing salt levels, an increase in mucus cells, limited onset hyperplasia, aneurysm, lamellar separation, and necrosis were observed in the gill tissue.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"1-13"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of the microalgae Tisochrysis lutea in a \"green water\" system increases the absorption area in the intestine of larvae of the seahorse Hippocampus reidi Ginsburg, 1933.","authors":"Elen Monique de Oliveira Sousa, Nayara Yoshimini de Oliveira, Ianka Agra da Silva, Renata Ávila Ozório, Emilly Monteiro Lopes, Maurício Laterça Martins, Mônica Yumi Tsuzuki","doi":"10.1007/s10695-024-01428-0","DOIUrl":"10.1007/s10695-024-01428-0","url":null,"abstract":"<p><p>The study aimed to investigate the influence of different microalgae used in green water culture on the intestinal morphology of the seahorse Hippocampus reidi during the first 15 days of life. Four treatments were tested in triplicate, consisting of two microalgae (Tisochrysis lutea-ISO and Chaetoceros muelleri-CHO), used either individually (TISO and TCHO) or combined (TIC 1:1) in larval rearing water, and a treatment without microalgae (TWM). Larvae (6.68 ± 0.55 mm) were fed copepods (Parvocalanus crassirrostris; 2 ind mL^-1) and rotifers (Brachionus rotundiformis; 8 ind mL^-1) from the first to the seventh day, and from the eighth day onwards, with Artemia sp. nauplii (2 ind mL^-1). For the analysis of intestinal histomorphometry, histological analysis of the tissue was performed on day 0 (initial) and after 7 and 15 days. The results demonstrate a significant increase in weight and weight gain of the larvae on the 15th day in the treatments with the addition of the microalgae T. lutea, whether alone or combination with other microalgae, compared to TWM. In relation the intestinal histomorphometry of larvae, the ANCOVA revealed that, on the 7th and 15th day, only the treatments with microalgae significantly affected the surface area of the intestinal villi. The intestinal histomorphometry of larvae from TISO showed higher values of villus height (85.29 ± 1.23 µm²) compared to TWM (69.42 ± 2.82 µm²) and TCHO (76.92 ± 2.23 µm²) (P < 0.05) but did not show significant differences compared to TIC (80.127 ± 2.08 µm²), which reflected on the villi surface area. Therefore, larvae from TISO (3603.43 ± 151.31 µm²) and TIC (3550.29 ± 120.99 µm²) showed significantly higher values of this parameter compared to TWM (3026.79 ± 147.64 µm²) and TCHO (3069.09 ± 126.18 µm²) (P < 0.05). In conclusion, the protocol for H. reidi larvae rearing in a green water system with the addition of the microalgae T. lutea resulted in an increase in the intestinal absorption area, resulting in improved performance of the larvae.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"1-12"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and sex affect respiratory responses to temperature and dissolved oxygen in the air-breathing fishes Betta splendens and Trichopodus trichopterus.","authors":"My Phuong Le, Warren Burggren, Gil Martinez-Bautista","doi":"10.1007/s10695-024-01411-9","DOIUrl":"10.1007/s10695-024-01411-9","url":null,"abstract":"<p><p>Ventilation frequencies of the gills (f_G) and the air-breathing organ (f_ABO) were measured in juveniles and adults of the air-breathing betta (Betta splendens) and the blue gourami (Trichopodus trichopterus) in response to temperature and hypoxia. Ventilatory rates were evaluated after 1 h of exposure to 27 °C (control), 23 and 31 °C (PO₂ = 21.0 kPa), after acute temperature changes (ATC) from 23 to 27, and 27 to 31 °C, and under progressive hypoxia (PH; PO₂ =  ~ 21 to 2.5 kPa). Complex, multi-phased ventilatory alterations were evident across species and experimental groups revealing different stress responses and shock reactions (e.g., changes in temperature sensitivity (Q₁₀) of f_G between 1-h exposure and ACT in both species). Female and male gourami showed differences in Q₁₀ over the temperature range 23-31 °C. No such Q₁₀ differences occurred in betta. Juveniles of both species showed higher Q₁₀ for f_ABO (~ 3.7) than f_G (~ 2.2). Adult fish exhibited variable Q₁₀s for f_G (~ 1.5 to ~ 4.3) and f_ABO (~ 0.8 to ~ 15.5) as a function of temperature, suggesting a switch from aquatic towards aerial ventilation in response to thermal stress. During PH, juveniles from both species showed higher f_G than adults at all oxygen levels. Females from both species showed higher f_G compared with males. Collectively, our results suggest that environmental cues modulate ventilatory responses in both species throughout ontogeny, but the actual responses reflect species-specific differences in natural habitat and ecology. Finally, we strongly suggest assessing physiological differences between male and female fish to avoid masking relevant findings and to facilitate results interpretation.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"1-22"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}