{"title":"An innovative perspective on fermented foods: isolation, purification, biochemical properties, and evaluation of the flavor formation potential of meat protein hydrolysis by Saccharomyces cerevisiae L3 proteases.","authors":"Yuhang Fan, Wenting Hui, Qian Liu, Jinxuan Cao, Sitong Liu, Fangda Sun, Baohua Kong","doi":"10.1016/j.foodchem.2025.145999","DOIUrl":"10.1016/j.foodchem.2025.145999","url":null,"abstract":"<p><p>This study evaluated the extracellular protease characteristics, protein hydrolysis activity, and flavor formation of Saccharomyces cerevisiae L3. The protease was purified using ammonium sulfate precipitation, size exclusion chromatography, and molecular sieve chromatography, yielding a relative molecular weight of 48.0 kDa. The protease exhibited maximum activity at pH 8 and 50 °C. The presence of Mg<sup>2+</sup> and Ca<sup>2+</sup> significantly enhanced the activity of protease (P < 0.05). The activity of protease was significantly inhibited by ethylene diamine tetraacetic acid (P < 0.05). The K<sub>m</sub> and V<sub>max</sub> of the protease were 17.84 mg/mL and 19.61 U/mL, respectively. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis revealed the protease's ability to hydrolyze myofibrillar proteins (MPs). Moreover, as protease hydrolysis progresses, the solubility of MPs, the levels of free amino acids, and volatile compounds all increase, thereby enhancing the flavor. The results demonstrated the potential application of S. cerevisiae protease in fermented meat products.</p>","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"493 Pt 3","pages":"145999"},"PeriodicalIF":9.8,"publicationDate":"2025-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-11-15Epub Date: 2025-08-22DOI: 10.1016/j.foodchem.2025.145940
Ruimin Wang, Lu Wang, Shengwei Wang, Juntao Wang, Congyan Su, Lin Zhang, Congfa Li, Sixin Liu
{"title":"Corrigendum to \"Phenolics from noni (Morinda citrifolia L.) fruit alleviate obesity in high fat diet-fed mice via modulating the gut microbiota and mitigating intestinal damage\" [Food Chem. 402 (2023) 134232].","authors":"Ruimin Wang, Lu Wang, Shengwei Wang, Juntao Wang, Congyan Su, Lin Zhang, Congfa Li, Sixin Liu","doi":"10.1016/j.foodchem.2025.145940","DOIUrl":"10.1016/j.foodchem.2025.145940","url":null,"abstract":"","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":" ","pages":"145940"},"PeriodicalIF":9.8,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chitosan/alginate biocomposites for postharvest preservation of horticultural produce: A comprehensive review","authors":"Shaojie Zheng, Fangting Ye, Yihui Chen, Hetong Lin, Yijing Wu, Chao Zhao","doi":"10.1016/j.foodchem.2025.146624","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.146624","url":null,"abstract":"Chitosan/alginate-based composites have demonstrated significant potential for preserving fruits and vegetables after harvest due to their superior mechanical properties, electrostatic interactions, and tunable barrier functionality. This review discusses various fabrication methods for chitosan/alginate composite systems, including layer-by-layer self-assembly, crosslinking agent-mediated film formation, post-blending gel coating, and nanocomposite films. These composite materials exhibit notable antimicrobial activity, which can be attributed to their ability to regulate enzymatic interference, disrupt electrostatic disruption, and facilitate ionotropic crosslinking. Additionally, their antioxidant efficacy arises from reactive oxygen species scavenging and enhances endogenous defenses (enzymes and non-enzymatic) in fresh produce. Furthermore, this review addresses the challenges of optimizing composites and highlights advancements in sustainable packaging, smart delivery systems, and eco-friendly preservation for next-generation postharvest management.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"105 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Metabolomics analysis reveals the formation mechanism of Cordyceps militaris fruiting bodies in liquid culture","authors":"Yihong Sun, Bingjie He, Xuemei Cao, Zimo Li, Lina Xu, Yanting Li, Shang Guo","doi":"10.1016/j.foodchem.2025.146651","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.146651","url":null,"abstract":"<em>Cordyceps militaris</em> is a functional fungus renowned for its significant nutritional and therapeutic value. However, the mechanisms governing its growth and fruiting body formation in liquid culture remain poorly understood. In this study, we employed UHPLC-MS/MS-based untargeted metabolomics to profile the metabolome of <em>C. militaris</em> across key developmental stages. A total of 2288 metabolites were identified in <em>C. militaris</em>. Among these, amino acids and derivatives accounted for the highest proportion, with 894 types identified. Organic acids are the second most abundant compounds, with 244 types identified. Metabolite profiles varied significantly across growth stages. Amino acids, organic acids, and lipids may serve as potential markers for distinguishing developmental stages. Notably, significant alterations were observed in six key metabolic pathways during mycelial proliferation, primordium differentiation, and fruiting body maturation. Our findings elucidate the metabolic drivers of <em>C. militaris</em> growth in liquid culture, providing a mechanistic basis for strain improvement and the industrial-scale production of bioactive metabolites.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"1 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-10-03DOI: 10.1016/j.foodchem.2025.146632
Nikkie van der Wielen, Hanlu Zhang, Pien J.C. Schouten, Erik Meulenbroeks, Natascha Stroebinger, Suzanne M. Hodgkinson, Marco Mensink, Wouter Hendriks, Edoardo Capuano
{"title":"Exploring in vitro production of colonic microbial metabolites from diverse protein sources using human ileal digesta","authors":"Nikkie van der Wielen, Hanlu Zhang, Pien J.C. Schouten, Erik Meulenbroeks, Natascha Stroebinger, Suzanne M. Hodgkinson, Marco Mensink, Wouter Hendriks, Edoardo Capuano","doi":"10.1016/j.foodchem.2025.146632","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.146632","url":null,"abstract":"We explored the relationship between protein fermentation metabolites and ileal digesta composition, using ileal digesta from ileostomates, who ingested nine different protein sources, incubated in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®). NH<sub>3</sub>, short-chain fatty acids, branched-chain fatty acids (BCFA), H<sub>2</sub>S, tryptophan derivatives, and biogenic amines were measured in proximal and distal colon vessels. The relative changes in most metabolites were positively correlated with their amino acid precursors in ileal digesta. In both colon vessels, the relative change of NH<sub>3</sub> was a good predictor for the production of other metabolites. Indole was strongly associated with oxindole, 5-HT, and tryptamine and the sum of Trp metabolites in the distal colon. Per gram ingested protein, zein and whey may produce the highest levels of NH<sub>3</sub> and BCFA in the proximal colon and BCFA in the distal colon, whereas whey and pigeon peas may result in the highest levels of H<sub>2</sub>S.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"98 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structural modifications and physicochemical characterization of Pueraria lobata starch: Focusing on the impact of combined enzymatic and acid hydrolysis on emulsifying efficiency","authors":"Fang Fang, Shuyu Cheng, Lihua Huang, Zijing Tian, Haiyang Gao, Mingwei Mu, Yongjian Cai","doi":"10.1016/j.foodchem.2025.146647","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.146647","url":null,"abstract":"With the increasing demand for green and sustainable materials, starch particles have attracted attention as Pickering stabilizers. This study investigated the structural, physicochemical, and emulsifying properties of <em>Pueraria lobata</em> starch (PLS) before and after Treatment (STs), including enzymatic hydrolysis via glucoamylase (GM) and α-amylase (AMG) or sulfuric acid hydrolysis, as well as the combined modification. Results showed that compared with PLS, all STs exhibited disrupted structure with enhanced wettability (13.22° ~ 44.73°) and surface charge. Combined modification significantly improved the emulsifying performance of STs. Notably, when the ratio of GM: AMG was 3:1 and sulfuric acid hydrolysis was conducted for 48 h (Gst <sub>G3A1 48</sub>), the contact angle increased by 44.73°. The emulsion prepared by Gst <sub>G3A1 48</sub> displayed significantly smaller droplet size (6.76 μm), improved viscosity and physical stability. These findings provide a new approach for developing high-performance, novel starch-based Pickering emulsifiers, widening its application in food industry.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"42 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-10-03DOI: 10.1016/j.foodchem.2025.146649
Sanja Ćavar Zeljković, Amra Brkić, Petr Tarkowski
{"title":"A high-throughput HILIC-UHPLC-MS/MS approach for multi-tissue profiling of alkaloids in Papaver somniferum: Method development and chemotypic prediction","authors":"Sanja Ćavar Zeljković, Amra Brkić, Petr Tarkowski","doi":"10.1016/j.foodchem.2025.146649","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.146649","url":null,"abstract":"A rapid and sensitive HILIC-UHPLC-MS/MS method was developed and validated for the simultaneous quantification of benzylisoquinoline alkaloids in <em>Papaver somniferum</em> tissues. The method fully separates six major alkaloids within 4.5 min using only 5 mg of dry plant material, with high recovery, minimal matrix effects, and excellent reproducibility across seeds, leaves, and capsules. Application to 15 food-grade poppy cultivars revealed significant chemotypic variation in seeds, as supported by profiling sugars, fatty acids, and phenolics. Time-course analysis in leaves and capsules uncovered distinct tissue-specific metabolic trends, with leaves serving as biosynthetic sources and capsules as primary sinks of alkaloids. Leaf metabolites at week 13 strongly predicted final alkaloid content in capsules, offering a non-destructive strategy for early screening. This high-throughput workflow supports chemotyping, cultivar screening, and quality control in food-grade poppy. Its sensitivity and low input make it ideal for both research and regulatory monitoring of alkaloids in food products.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"4 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impacts of gluten protein characteristics on the cracking behavior of dumpling wrappers during freeze-thaw cycles","authors":"Li-qin Tang, Xiaohong Sun, Xiao-Na Guo, Ke-Xue Zhu","doi":"10.1016/j.foodchem.2025.146650","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.146650","url":null,"abstract":"This study investigated the influence mechanism of gluten protein characteristics on the cracking behavior of dumpling wrappers (DWs) during freeze-thaw (FT) cycles. The results demonstrated that FT cycles increased freezable water content, water loss, and cracking rates. Five wheat flour varieties showed variation in cracking rates. DWs with the lowest cracking rate exhibited optimal moisture status. Gluten protein and rheological analysis illustrated that wheat flours with higher proportions of SDS-soluble polymers and monomeric proteins exhibited superior tensile resistance, whereas those with elevated SDS-insoluble protein content showed enhanced shear resistance. The lower gliadin/glutenin ratio in wheat flours contributes to enhanced deformation resistance. Microstructural observations indicated that low-cracking DWs exhibited better integrity than high-cracking counterparts. This study demonstrated that variations in gluten protein characteristics (gliadin/glutenin ratio and molecular weight distribution) mediate FT-induced cracking by influencing water variation and rheological behavior. These findings provide theoretical guidance for developing crack-resistant wheat flour of DWs.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"143 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}