{"title":"Effect of convective drying temperature and tray load density on bioactive compounds, antioxidant properties, and functional quality of radish Sango microgreens","authors":"Mahendra Gunjal, Atul Khalangre, Mansuri M. Tosif, Jyoti Singh, Sawinder Kaur, Riaz Ullah, Prasad Rasane","doi":"10.1016/j.foodchem.2025.144810","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.144810","url":null,"abstract":"This study examined the effects of convective drying parameters, including temperature (40, 50, and 60 °C) and tray loading density (0.057 and 0.113 g/cm<sup>2</sup>), on the bioactive compounds, antioxidant properties, and structural characteristics of radish sango microgreens powder. Drying at 50 °C with 0.057 g/cm<sup>2</sup> preserved the highest anthocyanins (43.23 μmol/100 g), phenolics (189.45 mg GAE/100 g), and antioxidant activities (DPPH 43.13 %, and FRAP 10.80 μmol TE/g), while drying at 50 °C with 0.113 g/cm<sup>2</sup> preserved the highest flavonoids (5.86 mg QUE/100 g) and ascorbic acid (239.18 mg/100 g) of radish sango microgreens. Degradation kinetics followed a first-order model, with thermal sensitivity varying among compounds. FTIR analysis revealed functional groups linked to bioactive compounds, while SEM showed flake-like particles enhancing solvent interaction. These findings highlight the significance of controlled drying in retaining functional and structural qualities, supporting the potential of radish sango microgreens in functional food and pharmaceuticals applications.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"233 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-05-17DOI: 10.1016/j.foodchem.2025.144773
Lingfang Zhang, Qianyi Jiang, Binghua Sun, Xiaoxi Wang, Sen Ma
{"title":"Designing food matrix structure by adjusting the ratio of soluble/insoluble wheat bran dietary fiber to mitigate the inhibition on wheat protein digestion: Application in noodles","authors":"Lingfang Zhang, Qianyi Jiang, Binghua Sun, Xiaoxi Wang, Sen Ma","doi":"10.1016/j.foodchem.2025.144773","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.144773","url":null,"abstract":"This study investigated the effect of changing the ratio of soluble (SDF) and insoluble (IDF) wheat bran dietary fiber on matrix structure and protein digestion in wheat noodles. Results showed that SDF at a low ratio (≤ 3.5) induced a compact matrix structure, which decreased protein digestibility to 67.70 % and total amino acids release to 140.67 mg/g protein, respectively. The compaction of the noodle matrix could be attributed to the promotion of cross-linking between D-type low molecular weight gluten and γ-gliadin by a small amount of SDF, reducing lacunarity from 0.28 to 0.16. A high SDF ratio (>3.5) induced a more fragmented matrix structure, increasing protein digestibility to 76.88 %. Notably, at a higher SDF ratio (>4.5), the concentration-dependent static quenching on enzymatic sites of SDF became prominent, slightly reducing gastric protein digestibility. In summary, an SDF ratio of 2.0–2.5 helps to reduce the inhibition of SDF on protein digestion.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"127 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-05-17DOI: 10.1016/j.foodchem.2025.144762
Shih-Wei Wu, Wei Hong Tai, Chia-Yu Hsieh, Biing-Hui Liu, Feng-Yih Yu
{"title":"Novel bispecific monoclonal antibody-based dual-analyte immunochromatographic strip for the detection of aflatoxin B1 and ochratoxin A in cereal samples","authors":"Shih-Wei Wu, Wei Hong Tai, Chia-Yu Hsieh, Biing-Hui Liu, Feng-Yih Yu","doi":"10.1016/j.foodchem.2025.144762","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.144762","url":null,"abstract":"We have developed a novel bispecific monoclonal antibody (mAb) for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA). The specific mAb was used to establish a sensitive competitive direct ELISA (cdELISA) and dual-analyte immunochromatographic strip (immunostrip). The cdELISA achieved IC<sub>50</sub> values of 0.06 ng/mL for AFB1 and 1.02 ng/mL for OTA, with limits of detection (LOD) of 0.004 ng/mL and 0.24 ng/mL, respectively. The dual-analyte immunostrip exhibited visual detection limits of 0.5 ng/mL for AFB1 and 5 ng/mL for OTA. Recovery rates for spiked cereal samples ranged from 91 % to 110 % for AFB1 and from 99 % to 107 % for OTA. The analysis of 15 cereal samples using the cdELISA detected AFB1 and OTA contamination consistent with HPLC analysis, validating the reliability of both methods. This bispecific mAb offers a rapid and efficient approach for monitoring AFB1 and OTA in food, enhancing food safety efforts.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"7 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impacts of soy protein isolate hydrolysates on gel properties of high-protein soy yogurt","authors":"Ziyi Li, Anan Xie, Yizhe Yan, Chunhong Piao, Aoxiang Lan, Siwen Wang, Jianfeng Wu, Xiang Fang","doi":"10.1016/j.foodchem.2025.144759","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.144759","url":null,"abstract":"In this study, the effects of incorporating soy protein isolate (SPI) and its hydrolysates (SPHs) on the gel properties of high-protein soy yogurt was investigated. Papain hydrolysis induced increased SPI solubility, reduced molecular weight and a more uniform size distribution as indicated by SDS-PAGE and SEC-HPLC. Formation of SPH aggregates were proven by increased particle size (4 μm) and decreased total free SH content and surface hydrophobicity. Soy yogurt with SPH addition at an appropriate degree of hydrolysis (DH; 1 to 3 %) exhibited reduced large protein aggregates and surface roughness, as observed by CLSM and SEM. Rheological and tribological analyses indicated that addition of SPHs reduced viscoelasticity and friction coefficient. However, excessive DH (4 to 6 %) resulted in low viscoelasticity that was difficult to maintain in a gel-like state, probably due to interference from preformed SPH aggregates and enhanced inter-protein electrostatic repulsion induced by raised pH and lowered isoelectric point.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"122 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Black tea addition enhances the aroma release of rose petals by activating related pathways during the scenting process","authors":"Yueling Zhao, Liuyi Chen, Jinlin Bian, Faxin Li, Yuanyuan Wang, Hongyu Zhou, Zihan Ma, Ping Xu, Xiao Du","doi":"10.1016/j.foodchem.2025.144801","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.144801","url":null,"abstract":"This study investigated the aroma-releasing mechanism of rose during the scenting process with black tea. By quantitative descriptive analysis of sensory evaluations, it was found that 20-h scenting optimized the floral fragrance accumulation while minimizing the undesirable green odors. Meanwhile, neryl alcohol, citronellol, rose oxide, geraniol, linalool, phenethyl alcohol, neryl acetate, citronellyl acetate, eugenol, methyleugenol, and phenethyl acetate were identified as the dominant contributors to the aroma profiles of rose black tea and rose petals. Notably, methylerythritol phosphate (MEP) metabolism, specifically enzymes such as 1-deoxy-D-xylulose-5-phosphate synthase (DXS), alcohol acyltransferase (AAT), and linalool synthase (LIS), and phenylalanine metabolism are the crucial pathways for the key aroma volatile synthesis. Interestingly, we found the introduction of black tea was able to early stimulate those enzymes and then activate the related metabolic pathways to facilitate the emission of rose aromatics, which were subsequently adsorbed by the tea base, yielding a floral-rich rose black tea.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"16 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-05-17DOI: 10.1016/j.foodchem.2025.144809
Eunwoo Jeong, Youjin Baek, Hyeon Gyu Lee
{"title":"Development and characterization of avenanthramide-loaded bilosomes: Bile salt-incorporated liposomes as a cholesterol substitute for enhanced oral delivery","authors":"Eunwoo Jeong, Youjin Baek, Hyeon Gyu Lee","doi":"10.1016/j.foodchem.2025.144809","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.144809","url":null,"abstract":"Avenanthramide (AVN), a phenolamide unique to oats, offers health benefits but is limited in application due to poor solubility, instability, and low bioavailability. This study developed bilosomes (AOE-BL) by incorporating bile salts into liposomes (AOE-LP) as a cholesterol substitute to enhance the oral delivery of AVN-enriched oat extract (AOE). AOE-BL exhibited smaller particle sizes and higher encapsulation efficiency than AOE-LP. Both formulations significantly increased AVN solubility by 2.74-fold and 2.71-fold, respectively, compared to free AOE while preventing heat-induced degradation. AOE-BL demonstrated superior stability under acidic conditions, with less pronounced changes in particle size and zeta potential than AOE-LP. After in vitro digestion, AOE-BL achieved significantly higher bioaccessibility (77.31 ± 3.31 %) than AOE-LP (67.21 ± 2.37 %) and free AOE (55.69 ± 3.43 %). Cellular uptake studies in Caco-2 enterocytes revealed that bile salts enhanced intracellular uptake compared to cholesterol. These findings highlight the potential of AOE-BL as an effective oral delivery system for AVN.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"75 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-05-17DOI: 10.1016/j.foodchem.2025.144807
Gan Hu, Xuan Yao, Xinyue Huang, Yuting Du, Wenlong Xu, Jinqiu Wang, Fang Geng
{"title":"Molecular mechanism of succinylation-mediated inhibition of heat-induced egg white protein aggregation: Structural characterization and molecular dynamics simulations","authors":"Gan Hu, Xuan Yao, Xinyue Huang, Yuting Du, Wenlong Xu, Jinqiu Wang, Fang Geng","doi":"10.1016/j.foodchem.2025.144807","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.144807","url":null,"abstract":"Thermal aggregation of food proteins poses a significant challenge in liquid food systems, limiting their practical applications in food processing and formulation. This study investigates the mechanism by which succinylation enhances the thermal stability of egg white proteins (EWP). The results demonstrated succinylation effectively prevented heat-induced aggregation (90 °C, 30 min), maintaining over 80 % light transmittance. Molecular dynamics simulations revealed that succinylation disrupted key salt bridges (eg., K62-E254, E14-K227) and increased polar surface area from 13,836 to 20,338 Å<sup>2</sup>, significantly altering protein-protein and protein-solvent interactions. These molecular changes were further confirmed by increased zeta potential (from −17.5 mV to −36.8 mV), enhanced β-sheet content (27.4 % to 40.0 %), and reduced surface hydrophobicity. As a result, the size of heat-induced aggregates decreased dramatically from 113.19 μm to 196.17 nm. These findings provide novel insights into the molecular basis of succinylation-enhanced protein thermal stability, offering valuable strategies for improving protein functionality in liquid food systems.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"8 1","pages":"144807"},"PeriodicalIF":8.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-05-17DOI: 10.1016/j.foodchem.2025.144811
Dijana Jelić, Milan Vraneš, Saša Zeljković, Suzana Veličković, Filip Veljković, Aleksandar Cvetkovski, Biljana Pejova, Snežana Papović
{"title":"Advancing food fortification with kinetics: Stabilizing vitamin D3 through calcium carbonate-based vehicles","authors":"Dijana Jelić, Milan Vraneš, Saša Zeljković, Suzana Veličković, Filip Veljković, Aleksandar Cvetkovski, Biljana Pejova, Snežana Papović","doi":"10.1016/j.foodchem.2025.144811","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.144811","url":null,"abstract":"The addition of vitamin D<sub>3</sub> in food products is an important health strategy. The vitamin D is heat, moisture, oxidation and pH sensitive. Given the complexity and diversity of food matrices, the use of various encapsulation strategies can more effectively address the need for vehicle system, that ensure the long-term chemical and physical stability of vitamin D. Calcium carbonate-based vehicle, modified with polyvinyl alcohol (PVA) and D-α-tocopherol succinate, were synthesized and loaded with vitamin D<sub>3</sub> (D<sub>3</sub>/X/CaCO<sub>3</sub>). The vehicle has a form of beadlet designed to protect vitamin D<sub>3</sub>. The obtained nanostructured D<sub>3</sub>/X/CaCO<sub>3</sub> demonstrated the desirable characteristics for powdered food matrix-vitamin D based, such as: enhanced thermal stability of vitamin D<sub>3</sub>, efficient loading, and an extended shelf-life. A detailed analysis of the physicochemical behavior of vitamin D, both structurally and kinetically, was provided presenting the fully functionalized pre-food model systems. The proposed kinetic perspective could serve as a roadmap for vitamin D₃ stability.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"35 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-05-17DOI: 10.1016/j.foodchem.2025.144775
Olga Vyviurska, Roksolana Fromel, Rocio Vidal Pellegrino, Adriano A. Gomes, Ivan Špánik
{"title":"Utilisation of stir Bar Sorptive extraction for the analysis of biogenic amines in wines","authors":"Olga Vyviurska, Roksolana Fromel, Rocio Vidal Pellegrino, Adriano A. Gomes, Ivan Špánik","doi":"10.1016/j.foodchem.2025.144775","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.144775","url":null,"abstract":"Wine intolerance primarily concerns the body's ability to process histamine, sulphites, tannins, and alcohol. Histamine, a biogenic amine, is involved in immune responses and helps regulate stomach acid. The legal limits for histamine in wine range from 2 to 10 mg/mL. Accurate estimation of histamine requires a sensitive and reliable sample preparation technique. In our study, we applied stir bar sorptive extraction (SBSE) with in situ derivatization using isobutyl chloroformate, followed by gas chromatographic analysis. Under optimal experimental conditions, established through multivariate design of experiments, a limit of detection (LOD) ranging from 5 to 17 μg/L was achieved. The developed SBSE method was used to compare the biogenic amine content in botrytized wines from different regions, including Slovakia, Hungary, France, and Austria. Principal component analysis revealed partial segregation of the samples from France and Hungary.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"13 1","pages":"144775"},"PeriodicalIF":8.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-05-17DOI: 10.1016/j.foodchem.2025.144791
Jie Zhong, Caie Wu, Tingting Li, Gongjian Fan, Dandan Zhou, Xiaojing Li
{"title":"Improving the quality of solidified yogurt with high-voltage electric field-treated chestnut short amylose/protein composite nanoparticles","authors":"Jie Zhong, Caie Wu, Tingting Li, Gongjian Fan, Dandan Zhou, Xiaojing Li","doi":"10.1016/j.foodchem.2025.144791","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.144791","url":null,"abstract":"Starch/protein composite nanoparticles have significant potential as gel modifiers for solidified yogurt. This study employed a high-voltage electric field to treat chestnut short amylose, which was then combined with three proteins to produce composite nanoparticles. The physicochemical properties of the nanoparticles and their effects on the quality of solidified yogurt were systematically investigated. The results indicated that high-voltage electric field treatment significantly improved starch nanoparticle uniformity, reducing size distribution from 0.375 to 0.172, and enhanced the thermal stability of short amylose-protein composite nanoparticles. Notably, the short amylose/soybean protein isolate (10:7.5, <em>w</em>/w) composite nanoparticles exhibited a peak temperature of 108.5 °C. When incorporated into solidified yogurt, the storage modulus at an angular frequency of 35 rad/s exceeded 85 Pa, representing a 131.75 % increase over the control. These findings underscore the potential of high-voltage electric field-treated short amylose/protein composite nanoparticles as highly effective gel modifiers for solidified yogurt.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"61 1","pages":"144791"},"PeriodicalIF":8.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}