ACS Applied Bio Materials最新文献

{"title":"Silica-Iron Oxide Magnetic Composite Particles for In Vitro Cell Separation: Synthesis, Possibilities, and Effects on Cell Metabolism.","authors":"Anna V Tyumentseva, Sergey V Komogortsev, Roman N Yaroslavtsev, Olga V Kryukova, Valeria V Petrova, Yulia V Pyastolova, Yuriy V Knyazev, Sergey V Stolyar","doi":"10.1021/acsabm.5c00587","DOIUrl":"https://doi.org/10.1021/acsabm.5c00587","url":null,"abstract":"<p><p>The development of magnetic particles for cell separation is a promising and actively developing direction. An important requirement for this method of isolation is the preservation of cell viability for the possibility of further study. The aim of the work was to develop magnetic composites based on iron oxide for single-stage cell separation and to evaluate the possibilities of further study of these cells using molecular and cellular biology methods. The particles were synthesized by the precipitation method; the magnetic cores were embedded in silica using TEOS and APTES reagents. Anti-EpCAM antibodies were immobilized on the surfaces of the obtained composites. T24 cells containing this antigen on the surface of some cells were used as a model suspension. It was shown that incubation of particles with the cells led to a decrease in the proportion of EpCAM-positive cells in the suspension and their binding to the magnetic composites. During the first hour of incubation with the particles, a decrease in the proportion of living cells in the suspension and a change in the mRNA level of the <i>BCL2</i> gene were noted. However, after 2 h of incubation, cell adaptation and restoration of viability were noted. The separation procedure resulted in a stable decrease in the expression of the <i>BIRC5</i> gene. The cells that were immobilized were subsequently successfully cultured. Thus, the proposed particles do not have high requirements for synthesis but allow for the isolation of living cells that can be used for further studies.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145051380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Action Nitric Oxide-Releasing Polydimethylsiloxane Sponge: Preventing Infection in Needleless Connectors.","authors":"Adam Brooks Goodman, Manjyot Kaur Chug, Natalie Crutchfield, Mark Garren, Hitesh Handa, Elizabeth J Brisbois","doi":"10.1021/acsabm.5c01100","DOIUrl":"https://doi.org/10.1021/acsabm.5c01100","url":null,"abstract":"<p><p>Catheter-related bloodstream infections (CRBSIs) are a prevalent concern, often resulting from suboptimal disinfection practices of needleless connectors. Although alcohol-based disinfectants have demonstrated efficacy, there is growing concern about developing microbial resistance. Similar to antibiotics in recent decades, microbes have the potential to develop resistance to these alcohol-based therapies. Therefore, this study delves into the antimicrobial potential of nitric oxide (NO), an endogenous gas molecule with broad-spectrum antimicrobial properties, in combination with the widely used disinfectant 70% isopropanol (IPA). Due to its short half-life, NO presents minimal risk of microbial resistance development. By incorporating <i>S</i>-nitroso-<i>N</i>-acetylpenicillamine (SNAP), a synthetic NO donor, into hydrophilic-modified polydimethylsiloxane (PDMS-PEO) sponges using 70% IPA, the sponge functions as an antimicrobial reservoir, effectively sterilizing the hub region of needleless connectors. Formulation-dependent effects on sponge porosity were observed, affecting compressive strength, absorption capacity, SNAP retention, and NO release kinetics. Up to 30% variation in sponge porosity coincided with a tunable compressive modulus, increased absorption capacity of 70% IPA, and enhanced SNAP loading after 15 min. These properties enable significantly greater SNAP release within 30 min. Zone of inhibition demonstrated higher porosity leads to more significant inhibition of <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, and <i>Candida albicans</i>. The disinfection of needleless connectors demonstrated a 2.91-, 7.04-, 2.02-, 3.21-, and 5.65-log reduction in <i>E. coli</i>, <i>P. aeruginosa</i>, <i>S. aureus</i>, <i>S. epidermidis</i>, and <i>C. albicans</i> viability after 30 min. These findings highlight the potential of this approach for efficient microbial decontamination in healthcare settings while offering adaptability for diverse biomedical applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Yellow Sodium Alginate Capable of Cross-Linking with Mg²⁺ to Form the Hydrogel of Magnesium Alginate Quickly and Easily.","authors":"Tongtong Pan, Xiao Wang, Lili Mao, Haizeng Wang","doi":"10.1021/acsabm.5c01224","DOIUrl":"https://doi.org/10.1021/acsabm.5c01224","url":null,"abstract":"<p><p>The bright yellow sodium alginate (YSA) powders were successfully prepared by a solid-phase reaction. The mechanism of the reaction and the structure of products have been investigated by Fourier transform infrared spectroscopy, Raman spectroscopy, and ¹H and ¹³C NMR spectroscopy. The terminal groups of the SA chains undergo ring-opening reactions under alkaline conditions, leading to the formation of an α-dicarbonyl group at the chain ends. Notably, for the first time, we discovered that YSA solution (M/G = 1:2) can rapidly and easily cross-link with Mg²⁺ to form magnesium alginate (MgA) hydrogel within 1 s and does not depend on the concentrations of SA or Mg²⁺. The MgA hydrogel exhibits stable, porous, and uniform structures. The YSA has the potential to replace existing applications of SA in alginate materials, enabling biocompatibility and other properties for applications in the medical, textile, and food industries.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145051366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable Surfactant-Free Synthesis of MnMoO₄/Carbon Nanofiber Composite for Highly Sensitive Detection of Nimesulide in Biological and Pharmaceutical Matrices.","authors":"Kumar Gokulkumar, Sakthivel Kogularasu, Shih-Hsuan Chen, Guo-Ping Chang-Chien, Wan-Ching Lin, Yung-Lung Chen, Kun-Mu Lee","doi":"10.1021/acsabm.5c01031","DOIUrl":"https://doi.org/10.1021/acsabm.5c01031","url":null,"abstract":"<p><p>In this study, a high-performance electrochemical sensor based on a MnMoO₄/carbon nanofiber (MnMoO₄/CNF) composite was developed for the sensitive and selective detection of nimesulide, a widely used nonsteroidal anti-inflammatory drug. The MnMoO₄ nanoparticles were synthesized via a deep eutectic solvent-assisted hydrothermal route and subsequently integrated onto acid-functionalized carbon nanofibers (CNFs) to form a hybrid electrocatalyst with enhanced conductivity and active surface area. Comprehensive physicochemical characterization using X-ray diffraction (XRD), Fourier transform infrared (FTIR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), and selected area electron diffraction (SAED) confirmed the successful formation of highly crystalline MnMoO₄ with uniform dispersion on the CNF matrix. Electrochemical investigations revealed that the MnMoO₄/CNF-modified glassy carbon electrode exhibited outstanding redox activity toward nimesulide, achieving a wide linear detection range of 0.00125-3525.25 μM, a low limit of detection of 0.0026 μM, high sensitivity 1.27 μA μM^-1 cm², and a high correlation coefficient (R² = 0.982). The sensor demonstrated favorable surface-controlled electrochemical kinetics, excellent stability over extended operation (1600 s), and high selectivity against common interfering substances such as phenols, uric acid, and glucose. Notably, the sensor exhibited remarkable reproducibility (RSD 98.3%) across independently fabricated electrodes. Real sample analyses in spiked blood plasma and pharmaceutical tablet extracts yielded satisfactory recoveries, validating the sensor's matrix compatibility and analytical robustness. The synergistic integration of electroactive MnMoO₄ and conductive CNF enables efficient electron transfer, high electrocatalytic activity, and structural stability, positioning the MnMoO₄/CNF composite as a promising candidate for practical applications in therapeutic drug monitoring and pharmaceutical quality assurance.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymerization-Induced Self-Assembly of Block Copolymers for Fabricating Polymeric Nanomaterials with Inverse Morphologies.","authors":"Guangzhao Li, Wang Gao, Hongyan Cao, Yuxuan Liu, Jiaqi Yu, Hongxiao Zhang, Ke Huang, Ulrich Glebe, Liang Qiu, Chengfen Xing","doi":"10.1021/acsabm.5c01400","DOIUrl":"https://doi.org/10.1021/acsabm.5c01400","url":null,"abstract":"<p><p>Inverse nanostructures offer unique structural features, including multicompartmental organization and high surface area, rendering them promising for applications as porous materials and for molecular separations, catalysis, and biointerfaces. However, inverse nanostructures constructed via block copolymer self-assembly suffer from low concentrations, multistep processing, limited scalability, and morphological complexity. Polymerization-induced self-assembly (PISA) offers a powerful alternative, enabling the efficient, one-pot fabrication of nanostructures with tunable morphologies at high solid contents. This review highlights recent advances in using PISA to access inverse morphologies, summarizing key parameters, including block composition, monomer design, and solvent selectivity, that govern structure formation. This review also presents several illustrative examples that demonstrate the practical applications of inverse morphologies synthesized through the PISA approach, thereby illuminating future opportunities for this innovative technique.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Engineering by Micropatterned Polymer Brushes: Strategies to Construct and Their Applications in Biomedical Areas.","authors":"Meenakshi Verma, Shubham Roy, Sampa Saha","doi":"10.1021/acsabm.5c01387","DOIUrl":"https://doi.org/10.1021/acsabm.5c01387","url":null,"abstract":"<p><p>Micropatterned polymer brushes are a revolutionary platform that has opened up unique biomedical application possibilities through spatial control of surface characteristics. Unlike traditional polymer-brush coatings that enable homogeneous surface modifications, micropatterned brushes allow the fabrication of heterogeneous surfaces by providing localized functionality for enhanced biological interactions. This review presents different lithography techniques followed by surface-initiated polymerization as advanced fabrication methods that are vital in creating such a modulated surface architecture. Despite remarkable achievements, issues of scalability and reproducibility of the created surfaces remain a core challenge toward their industrial utilization. By critically evaluating these methodologies and their integration with biomedical technologies, we have outlined the current limitations and potential breakthroughs required for the future deployment of micropatterned polymer brushes in healthcare, particularly in areas such as biosensing, drug delivery, and tissue engineering.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Nanozymes and Their Composites for Antibiofilm Applications.","authors":"Hui Cui, Yuanyuan Cui, Huanxiang Yuan, Ruilian Qi","doi":"10.1021/acsabm.5c01274","DOIUrl":"https://doi.org/10.1021/acsabm.5c01274","url":null,"abstract":"<p><p>Biofilm-associated infections caused by microbial communities have become a major threat to the global public health. Once formed, biofilms not only significantly enhance microbial resistance to antibiotics but also render infections extremely difficult to eradicate, often resulting in poor clinical outcomes and high mortality rates. Therefore, there is an urgent need to develop effective antibiofilm strategies to combat these persistent infections. Nanozymes, nanomaterials with intrinsic enzyme-like catalytic activities, have attracted increasing attention as promising tools for combating biofilm-related infections. As artificial enzyme mimics, nanozymes exhibit excellent catalytic efficiency, enabling them to rapidly generate large amounts of reactive oxygen species (ROS) or catalyze the hydrolysis of biomolecules within microbial cells and biofilms, thereby effectively preventing biofilm formation or eradicating established biofilm. Combined with their good biocompatibility and stability, nanozymes have made remarkable progress in antibiofilm applications over the past five years. In this review, we first provide a brief overview of biofilm infections, biofilm structure, and the mechanisms underlying their antibiotic resistance. Then, we summarize recent advances in the application of nanozymes and their composites to the prevention and disruption of microbial biofilms. Finally, we briefly summarize the present status of nanozymes in antibiofilm research, discuss existing challenges, and propose future prospects for nanozymes in combating biofilms.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perovskite Nanozyme-Mediated Sonocatalytic Therapy: A Mitochondrion-Targeted Strategy for Enhanced Cancer Therapy.","authors":"Li Yang, Jialun Li, Xiaomin Sun, Wangzixi Zhang, Tiedong Sun, Yuan Sun, Jinyu Wu, Lei Wang","doi":"10.1021/acsabm.5c01087","DOIUrl":"https://doi.org/10.1021/acsabm.5c01087","url":null,"abstract":"<p><p>The generation of reactive oxygen species (ROS) through nanozyme-mediated sonocatalytic therapy has demonstrated remarkable therapeutic efficacy in the field of cancer. Nevertheless, it remains a significant challenge for nanozymes with a single catalytic active center to generate sufficient ROS via Fenton or Fenton-like reactions to effectively induce tumor cell death. In order to enhance the catalytic efficacy, we devised and synthesized a multiple active centre and mitochondrial-targeted perovskite nanozyme (NCFP), doped with cobalt (Co) element, and incorporated 4-carboxybutyltriphenylphosphonium bromide (TPP) as a mitochondrial targeting marker for ultrasound (US)-assisted enzyme-like catalytic treatment of tumors. Considering that perovskite nanozymes have the advantages of long carrier diffusion length, tunable direct band gap, and strong quantum confinement, it can be used as a substrate to increase the production rate of ROS. Therefore, on the basis of the intrinsic catalytic reaction, US is introduced to improve the catalytic reaction efficiency. The cavitation effect releases energy to promote the electron-hole pair separation of NCFP, increase the rate of electron-hole-mediated reaction, and enhance the degree of reaction, thereby helping it to produce more ROS and effectively kill tumor cells. Moreover, NCFP also has mitochondrial targeting ability, which can damage mitochondria more accurately and kill tumor cells. In summary, the successful preparation of NCFP provides a strategy for perovskite nanozyme-mediated sonocatalytic therapy for tumor therapy.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mannose-Decorated Stimuli-Responsive Fluorescent Poly(aminoamide) Microgels for Lectin Binding and Sensing.","authors":"Aayush Anand, Amandeep Singh, Soumen Ghosh, Subrata Chattopadhyay","doi":"10.1021/acsabm.5c01081","DOIUrl":"https://doi.org/10.1021/acsabm.5c01081","url":null,"abstract":"<p><p>Development of suitable carbohydrate-decorated, biocompatible, and stimuli-responsive fluorescent microgels that can selectively bind and detect proteins (such as lectins) is an important research topic. Herein, we report the development of mannose-decorated, dual-stimuli (temperature and pH)-responsive fluorescent poly(aminoamide) microgels, which can selectively bind to and thereby detect the presence of concanavalin A (Con A). The resultant stimuli-responsive microgels have a lower critical solution temperature (VPTT) of 37.4 °C and exhibit pH responsiveness over a wide range. The microgels exhibited robust multivalent interactions with Con A. Binding kinetics, as shown by fluorescence quenching, demonstrated a rapid response within 8 min, with a detection limit of 15 nM and a binding constant (<i>K_d</i>) of 3.77 × 10⁶ M^-1.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Brain-Inspired Hierarchical Micro-Nanostructured Poly(3,4-ethylenedioxythiophene)/Polydopamine Neural Interface on Titanium Nitride Electrodes for Electrophysiological Signal Recording.","authors":"Shahab Ahmadi Seyedkhani, Azam Iraji Zad, Raheleh Mohammadpour, Mojtaba Taghipoor, Mohaddeseh Vafaiee","doi":"10.1021/acsabm.5c01451","DOIUrl":"https://doi.org/10.1021/acsabm.5c01451","url":null,"abstract":"<p><p>The development of high-performance neural interfaces is critical for advancing brain-machine communication and treating neurological disorders. A major challenge in neural electrode design is achieving a seamless biological-electronic interface with optimized electrochemical properties, mechanical stability, and biocompatibility. In this study, we introduce a hierarchical micronanostructured poly(3,4-ethylenedioxythiophene)-polydopamine (PEDOT-PDA) coating on titanium nitride (TiN) microelectrodes engineered to enhance electrophysiological signal recording and neural integration. The PEDOT-PDA films were synthesized via potentiodynamic electropolymerization, achieving a 90% reduction in impedance (∼353 Ω at 1 kHz) compared to conventional gold (Au) electrodes (∼3795 Ω) and a 60% decrease relative to TiN substrates (∼890 Ω). The brain-inspired hierarchical micronanostructure mimics the extracellular matrix (ECM), improving cell adhesion and biointegration. Wettability analysis revealed a 63% enhancement in hydrophilicity, reducing the water contact angle from ∼70° for pure PEDOT to ∼25° for PEDOT-PDA. Biocompatibility assessments demonstrated excellent cell viability of ∼97% for PEDOT-PDA electrodes and superior cell attachment with extended filopodia formation, promoting long-term neural interface stability. The PEDOT-PDA interface outperforms conventional PEDOT and metal-based electrodes in electrochemical stability, biocompatibility, and signal recording efficiency, making it a promising candidate for next-generation brain-computer interfaces (BCIs).</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}