ACS Applied Bio Materials最新文献

{"title":"Next-Generation Point-of-Care Diagnostics: Silver Nanoparticle-Enhanced 3D-Printed Multiplex Electrochemical Biosensor for Detecting Dengue and Chikungunya Viruses.","authors":"Pradakshina Sharma, Mohd Rahil Hasan, Aysenur Yardim, Emine Guler Celik, Suna Timur, M Z Abdin, Jagriti Narang","doi":"10.1021/acsabm.4c01530","DOIUrl":"https://doi.org/10.1021/acsabm.4c01530","url":null,"abstract":"<p><p>In recent years, the increasing prevalence of viral infections such as dengue (DENV) and chikungunya (CHIKV) has emphasized the vital need for new diagnostic techniques that are not only quick and inexpensive but also suitable for point-of-care and home usage. Existing diagnostic procedures, while useful, sometimes have limits in terms of speed, mobility, and price, particularly in resource-constrained environments and during epidemics. To address these issues, this study proposes a novel technique that combines 3D printing technology with electrochemical biosensors to provide a highly sensitive, user-friendly, and customizable diagnostic platform. This study focuses on a unique 3D-printed electrode cassette made with fused deposition modeling technology, which ensures strong structural alignment and improved performance under a variety of environmental conditions. When combined with paper-based electrodes loaded with silver nanoparticles, the platform dramatically enhances the detection sensitivity and reliability. The biosensor uses cyclic voltammetry and electrochemical impedance spectroscopy to detect DENV and CHIKV antigens within a linear range of 1 × 10² to 1 × 10⁶ ng/mL. Results were delivered in 20 s and stable for 30 days. The device's performance was verified by testing with blood serum samples containing both DENV and CHIKV antigens, demonstrating its capacity to properly identify coinfections. This novel diagnostic tool represents a huge step forward in accessible and efficient healthcare solutions, bridging important gaps in the global battle against arboviral infections.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances of Carbon Dots: Synthesis, Plants Applications, Prospects, and Challenges.","authors":"Zhiwei Zhang, Caijuan Wu, Juan Hu, Chen Li, Yingliang Liu, Bingfu Lei, Mingtao Zheng","doi":"10.1021/acsabm.4c01785","DOIUrl":"https://doi.org/10.1021/acsabm.4c01785","url":null,"abstract":"<p><p>Nanomaterials and nanotechnology have garnered significant attention in the realm of agricultural production. Carbon dots (CDs), as a class of nanomaterials, play a crucial role in the field of plant growth due to their excellent properties. This review aims to summarize recent achievements on CDs, focusing on their methods of preparation and applications in plants systems. The effects of CDs on seed germination, growth, photosynthesis, nutritional quality, and stress resistance were studied. It has been demonstrated that CDs can promote seed germination and growth, as well as improve photosynthetic efficiency, ultimately leading to increase plants yield. The nutritional quality of the plants treated with CDs was significantly improved. Specifically, the levels of essential mineral elements, vitamins, amino acids, and other constituents that are beneficial to human health increased notably. Additionally, CDs show positive effects on augmenting plant resistance against environmental stresses, such as drought conditions, heavy metal toxicity, and high salinity. Finally, the prospects and challenges of the application of CDs in plant systems are also discussed, which provide a scientific basis for the future application of CDs in agricultural production.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun Chitosan/Polylactic Acid Nanofibers with Silver Nanoparticles: Structure, Antibacterial, and Cytotoxic Properties.","authors":"Yevhen Samokhin, Yuliia Varava, Kateryna Diedkova, Ilya Yanko, Valeriia Korniienko, Yevheniia Husak, Igor Iatsunskyi, Vladlens Grebnevs, Maris Bertiņs, Rafal Banasiuk, Viktoriia Korniienko, Agne Ramanaviciute, Maksym Pogorielov, Arunas Ramanavicius","doi":"10.1021/acsabm.4c01252","DOIUrl":"https://doi.org/10.1021/acsabm.4c01252","url":null,"abstract":"<p><p>Electrospinning, a technique for creating fabric materials from polymer solutions, is widely used in various fields, including biomedicine. The unique properties of electrospun fibrous membranes, such as large surface area, compositional versatility, and customizable porous structure, make them ideal for advanced biomedical applications like tissue engineering and wound healing. By considering the high biocompatibility and well-known regenerative potential of polylactic acid (PLA) and chitosan (CH), as well as the versatile antibacterial effect of silver nanoparticles (AgNPs), this study explores the antibacterial efficacy, adhesive properties, and cytotoxicity of electrospun chitosan membranes with a unique nanofibrous structure and varying concentrations of AgNPs. Silver nanoparticles incorporated at concentrations of 25-50 μg/mL or above significantly enhanced the antibacterial effectiveness, especially against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. Biocompatibility assessments using umbilical cord mesenchymal stem cells demonstrated the nontoxic nature of the membranes with an AgNP concentration of 12.5 μg/mL, underscoring their potential for biomedical applications. This study provides valuable insights into developing electrospun chitosan membranes as effective antimicrobial coatings for various biomedical uses, including wound healing patches and tissue engineering constructs for soft tissue replacement.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ru^III-Morpholine-Derived Thiosemicarbazone-Based Metallodrugs: Lysosome-Targeted Anticancer Agents.","authors":"Monalisa Mohanty, Sanchita Das, Pratikshya Das Pattanayak, Sudhir Lima, Werner Kaminsky, Rupam Dinda","doi":"10.1021/acsabm.4c01536","DOIUrl":"https://doi.org/10.1021/acsabm.4c01536","url":null,"abstract":"<p><p>The idea of coordinating biologically active ligand systems to metal centers to exploit their synergistic effects has gained momentum. Therefore, in this report, three Ru^III complexes <b>1</b>-<b>3</b> of morpholine-derived thiosemicarbazone ligands have been prepared and characterized by spectroscopy and HRMS along with the structure of <b>2</b> through a single-crystal X-ray diffraction study. The solution stability of <b>1</b>-<b>3</b> was tested using conventional techniques such as UV-vis and HRMS. Further, the anticancer activity of <b>1</b>-<b>3</b> was tested in HT-29 and HeLa cancer cell lines. To gain insight into their mechanism of action, the cytotoxicity, hydrophobicity, and the interaction of <b>1</b>-<b>3</b> with DNA and HSA were evaluated by different conventional methods such as absorption, fluorescence, and circular dichroism studies. Along with favorable biomolecule interaction, <b>1</b>-<b>3</b> revealed potent selectivity toward cancer cells, which is a prerequisite for the generation of an anticancer drug. According to cell viability results, <b>1</b> has the highest cytotoxicity among all in the group, against both cells, respectively. Additionally, the fluorescence-active ruthenium complexes selectively target lysosomes, which is evaluated by live-cell imaging. <b>1</b>-<b>3</b> disrupt the lysosome membrane potential by generating an excessive amount of reactive oxygen species, which results in an apoptotic mode of cell death.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Ex Vivo Evaluation of a PCLA Degradable Device To Improve Annulus Fibrosus Repair.","authors":"Chloé Falcoz, Mansoor Chaaban, Cédric Paniagua, Marion Fusellier, Jérôme Guicheux, Catherine Le Visage, Benjamin Nottelet, Xavier Garric, Coline Pinese","doi":"10.1021/acsabm.4c01415","DOIUrl":"https://doi.org/10.1021/acsabm.4c01415","url":null,"abstract":"<p><p>With a prevalence of over 90% in people over 50, intervertebral disc degeneration (IVDD) is a major health concern. This weakening of the intervertebral discs can lead to herniation, where the nucleus pulpus (NP) leaks through the surrounding Annulus Fibrosus (AF). Considering the limited self-healing capacity of AF tissue, an implant is needed to restore its architecture and function. Here, we developed a biomimetic electrospun nanofibrous biodegradable scaffold that could be potentially used to repair AF defects. To that aim, we synthesized copolymers and blends of ε-caprolactone and lactide to create poly(ε-caprolactone-<i>co</i>-lactide) (PCLA) and PCL/PLA scaffolds with 10, 20, or 30% PLA. Properties of the initial nanofibrous scaffolds and the impact of gamma irradiation sterilization on the mechanical, thermal, and <i>in vitro</i> degradation properties are assessed and discussed with respect to the AF application. It was shown that ovine AF cells colonize the nanofibrous layers with increased metabolic activity over time. As an outcome of these studies, two copolymers were chosen to design a device composed of a 3D nanofibrous stacked scaffold associated with a degradable anchoring system to maintain the scaffold in an AF defect. The implantability of this device was tested in a cadaveric sheep lumbar IVD.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"M3-DPPE Liposomal Nanoparticles Encapsulating CLEC12A Enhance CD206-Mediated Endocytosis and Efficacy in the Collagen-Induced Arthritis Model.","authors":"Shulin Luo, Junfeng Cai, Feng Yin, Laiya Lu, Zheng Liu, Yunxia Wang, Xiaocong Fu, Shuangfeng Ding, Naoya Kojima, Min Ma","doi":"10.1021/acsabm.4c01139","DOIUrl":"https://doi.org/10.1021/acsabm.4c01139","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the efficacy of M3-DPPE liposomal nanoparticles encapsulated with mRNA encoding cytokines (M3-mRNAs) in targeting macrophages for the treatment of inflammation-induced joint injury.</p><p><strong>Methods: </strong><i>in vitro</i>, M3-mRNAs were administered to peritoneal exudate macrophages (PEMs), and the uptake was assessed using flow cytometry. The mechanism of uptake was investigated by blocking the CLEC12A pathway with M3-SiCLEC12A and observing CD206-mediated endocytosis. In vivo, the distribution of Dir-labeled M3-drugs was monitored using IVIS imaging, and its accumulation in inflammatory and noninflammatory areas was evaluated. The therapeutic potential was evaluated in collagen-induced arthritis (CIA) model mice by assessing macrophage polarization, joint pathology, and cytokine expression.</p><p><strong>Results: </strong><i>in vitro</i> studies demonstrated that M3-mRNAs were taken up significantly by PEMs via CD206-mediated endocytosis. In vivo imaging showed that Dir-labeled M3-drugs accumulated predominantly in inflammatory areas and subsequently in bone injury joints. Treatment with M3-drugs in collagen-induced arthritis model mice increased the population of F4/80+ and F4/80+/CD206+ M2 macrophages in inflamed joints, leading to reduced joint fibrosis and modulation of cytokine levels, including decreased pro-inflammatory cytokines (IL-6, IL-1β, TNF-α, and INF-γ) and increased anti-inflammatory cytokines (IL-10 and TGF-β).</p><p><strong>Conclusions: </strong>M3-SiCLEC12A enhanced CD206-mediated endocytosis of M3-mRNAs and M3-drugs in macrophages, promoting the production of corresponding proteins and modulating the immune microenvironment. This treatment approach shows promise in repairing inflammation-induced bone and joint injury by balancing pro-inflammatory and anti-inflammatory cytokines. However, further research is required to address drug tolerance and safety concerns and minimize potential side effects before clinical application in autoimmune diseases caused by inflammation.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Potential of Nanocarriers for Cancer Immunotherapy: Insights into Mechanism, Nanocarriers, and Regulatory Perspectives.","authors":"Tanmoy Kanp, Anish Dhuri, Bharath M, Khushi Rode, Mayur Aalhate, Priti Paul, Rahul Nair, Pankaj Kumar Singh","doi":"10.1021/acsabm.4c01797","DOIUrl":"https://doi.org/10.1021/acsabm.4c01797","url":null,"abstract":"<p><p>Immunotherapy is a cutting-edge approach that leverages sophisticated technology to target tumor-specific antibodies and modulate the immune system to eradicate cancer and enhance patients' quality of life. Bioinformatics and genetic science advancements have made it possible to diagnose and treat cancer patients using immunotherapy technology. However, current immunotherapies against cancer have limited clinical benefits due to cancer-associated antigens, which often fail to interact with immune cells and exhibit insufficient therapeutic targeting with unintended side effects. To surmount this challenge, nanoparticle systems have emerged as a potential strategy for transporting immunotherapeutic agents to cancer cells and activating immune cells to combat tumors. Consequently, this process potentially generates an antigen-specific T cells response that effectively suppresses cancer growth. Furthermore, nanoplatforms have high specificity, efficacy, diagnostic potential, and imaging capabilities, making them promising tools for cancer treatment. However, this informative paper delves into the various available immunotherapies, including CAR T cells therapy and immune checkpoint blockade, cytokines, cancer vaccines, and monoclonal antibodies. Furthermore, the paper delves into the concept of theragnostic nanotechnology, which integrates therapy and diagnostics for a more personalized treatment approach for cancer therapy. Additionally, the paper covers the potential benefits of different nanocarrier systems, including marketed immunotherapy products, clinical trials, regulatory considerations, and future prospects for cancer immunotherapy.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Chirality and Amphiphilicity on the Antimicrobial Activity of Tripodal Lysine-Based Peptides.","authors":"Anindyasundar Adak, Valeria Castelletto, Lucas de Mello, Bruno Mendes, Glyn Barrett, Jani Seitsonen, Ian W Hamley","doi":"10.1021/acsabm.4c01635","DOIUrl":"https://doi.org/10.1021/acsabm.4c01635","url":null,"abstract":"<p><p>A series of tripodal (three-arm) lysine-based peptides were designed and synthesized and their self-assembly properties in aqueous solution and antimicrobial activity were investigated. We compare the behaviors of homochiral tripodal peptides (KKY)₃K and a homologue containing the bulky aromatic fluorenylmethoxycarbonyl (Fmoc) group Fmoc-(KKY)₃K, and heterochiral analogues containing k (d-Lys), (kkY)₃K and Fmoc-(kkY)₃K. The molecular conformation and self-assembly in aqueous solutions were probed using various spectroscopic techniques, along with small-angle X-ray scattering (SAXS) and cryogenic-transmission electron microscopy (cryo-TEM). In cell viability assays using fibroblast cell lines, the tripodal peptides without Fmoc were observed to be noncytotoxic over the concentration range studied, and the Fmoc functionalized tripodal peptides were only cytotoxic at the highest concentrations (above the critical aggregation concentration of the lipopeptides). The molecules also show good hemocompatibility at sufficiently low concentration, and antimicrobial activity was assessed via MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) assays. These revealed that the Fmoc-functionalized tripodal peptides had significant activity against both Gram-negative and Gram-positive bacteria, and in the case of Gram-positive <i>Staphylococcus aureus</i>, the antimicrobial activity for Fmoc-(kkY)₃K was improved compared to polymyxin B. The mechanism of the antimicrobial assay was found to involve rupture of the bacterial membrane as evident from fluorescence microscopy live/dead cell assays, and scanning electron microscopy images.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flame Retardancy and Heat Shielding in Green Polypropylene Composites Using Biohybrid Fibers and Agro-Waste Fillers.","authors":"Thanh Mai Nguyen Tran, M N Prabhakar, Lee Dong Woo, Song Jung-Il","doi":"10.1021/acsabm.4c01733","DOIUrl":"https://doi.org/10.1021/acsabm.4c01733","url":null,"abstract":"<p><p>The current work presents the flame-retardant performance of hybrid polypropylene composites, reinforced with specific short woven flax fabrics (SWFs), short basalt fibers (BFs), and rice husk powder (RHP), using polypropylene grafted maleic anhydride (MAPP) as the coupling agent. Horizontal burning test (HBT), microcalorimeter test (MCT), and cone calorimeter test (CCT) were conducted on these composites. The formulations used were 25% SWF/PP, 25% SWF/20% BF/PP, and 25% SWF/20% BF/PP with 6% RHP and 25% SWF/20% BF/PP with varying RHP contents (6, 12, and 18%) in combination with 6% MAPP. A peak heat release rate (pHRR) reduction of 14.42% was recorded in microcalorimeter test results. However, cone calorimeter test results indicated a reduction of 80.95% in pHRR and 23.84% in total heat release rate (THR). Furthermore, the emissions of CO and CO₂ were reduced by 68 and 67%, respectively, in the 25SWF/20BF/PP.6MAPP-18RHP composite compared to pure PP, indicating an overall improvement in combustion efficiency. Fire performance index (FPI) also improved remarkably: FPI increased by 130% and fire growth index (FGI) improved by 81.34%. Moreover, the horizontal burning test showed an improved performance, with the burning rate further reduced by 11.10% compared to that of the 25% SWF/PP composite. These results highlight the synergy among natural fibers, agro-waste fillers, and MAPP in improving flame retardancy of polypropylene composites, and hence their potential application in automobiles and construction, where the demand for fire safety is very high.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Situ Ag-Seeded Lamellar Ti₃C₂ Nanosheets: An Electroactive Interface for Noninvasive Diagnosis of Oral Carcinoma via Salivary TNF-α Sensing.","authors":"Sunil Vasu, Damini Verma, Omal Surya Souraph S, Katha Anki Reddy, Gopinath Packirisamy, Uday Kumar S","doi":"10.1021/acsabm.4c01379","DOIUrl":"https://doi.org/10.1021/acsabm.4c01379","url":null,"abstract":"<p><p>In the fast-paced quest for early cancer detection, noninvasive screening techniques have emerged as game-changers, offering simple and accessible avenues for precession diagnostics. In line with this, our study highlights the potential of silver nanoparticle-decorated titanium carbide MXene nanosheets (Ti₃C₂_AgNPs) as an electroactive interface for the noninvasive diagnosis of oral carcinoma based on the prevalence of the salivary biomarker, tumor necrosis factor-α (TNF-α). An in situ reduction was utilized to synthesize the Ti₃C₂_AgNPs nanohybrid, wherein Ti₃C₂ acts as the reducing agent, and the resulting nanohybrid was subjected to various characterization techniques to examine the optical, structural, and morphological attributes. The results revealed that spherical AgNPs formed on the surface of Ti₃C₂ MXene nanosheets by virtue of the low-valent Ti species present in Ti₃C₂, which facilitated the reduction of AgNO₃ to AgNPs. Furthermore, the electrochemical characterization of the nanohybrid-modified screen-printed electrode (Ti₃C₂_AgNPs/SPE) indicated enhanced heterogeneous electron transfer kinetics. With these encouraging results, the Ti₃C₂_AgNPs nanohybrid was employed as an immobilization matrix for TNF-α antibodies and applied for electrochemical sensing. Analytical studies of the fabricated immunosensor, conducted by differential pulse voltammetry (DPV), exhibited a broader linear range (1 to 180 pg mL^-1), a low limit of detection (0.97 pg mL^-1), and high sensitivity (1.214 μA mL pg^-1 cm^-2) and specificity, even in artificial saliva, indicating its reliability for oral carcinoma diagnosis. Therefore, the Ti₃C₂_AgNP nanohybrid seems a promising candidate for the effective sensing of TNF-α and could also be explored for other biomarkers.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}