ACS Applied Bio Materials最新文献

{"title":"Lab to Fab Process Using Ablation Lasers: A Lightweight, Flexible, and Biocompatible Microheater for Wearable Therapy Applications.","authors":"Bhavani Prasad Yalagala, Tahereh Masalehdan, Changhao Ge, Mahmut Talha Kirimi, John Mercer, Morteza Amjadi Kolour, Hadi Heidari","doi":"10.1021/acsabm.5c01263","DOIUrl":"https://doi.org/10.1021/acsabm.5c01263","url":null,"abstract":"<p><p>Wearable thermal therapy has attained significant attention owing to its potential in various wearable and biomedical applications specifically targeted for accelerated wound healing using smart bandages. Here, in the current work, a large area, precision, and scalable fabrication methodology is proposed using an ultraviolet (UV)-based ablation laser as compared to conventional fabrication methods such as photolithography or printing. This laser-based approach is unique and offers rapid prototyping, superior material versatility, design flexibility, and minimal thermal damage to the emerging biocompatible polymer-based flexible substrates. A thin, flexible, and biocompatible microheater and an array (4 × 4) of diverse designs, including circular, hexagonal, and planar, were designed and fabricated on a gold-coated PAC substrate using the proposed ablation laser-based approach. Multiple heater sizes varying from small to extra-large were fabricated and are tailored for the targeted temperatures ranging from 30 °C to 100 °C for biomedical applications, especially wearable wound healing applications. Electrical and electrothermal characteristics revealed that the sheet resistance, thermal response, and response time vary with the structure and size of the microheaters. Further, mechanical flexibility and biocompatibility studies on the PAC, patterned gold electrodes, and polyimide substrate demonstrated excellent mechanical robustness and biocompatibility, clearly demonstrating its efficacy and ability for wearable and implantable applications. Finally, the proposed research paves the pathway for the fabrication of next-generation wearable and implantable biointegrated flexible microheater devices toward advanced thermal therapy solutions.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145311916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat-Induced Structural Changes in Lactoferrin for Enhanced Mucoadhesion.","authors":"Bianca Hazt, Daniel J Read, Oliver G Harlen, Wilson C K Poon, Adam O'Connell, Simon D Connell, Anwesha Sarkar","doi":"10.1021/acsabm.5c01534","DOIUrl":"https://doi.org/10.1021/acsabm.5c01534","url":null,"abstract":"<p><p>The development of biocompatible and safe mucoadhesive materials is critical for improving therapeutic strategies, where cationic proteins such as lactoferrin are emerging as promising alternatives to synthetic polymers. Here, we demonstrate how thermal denaturation of lactoferrin can be used as a viable strategy to enhance mucoadhesion. We identify and study in detail the structural changes in lactoferrin upon thermal denaturation using light scattering, circular dichroism spectroscopy, gel-electrophoresis, and atomic force microscopy. Lactoferrin-mucin binding was evaluated using rheology, confocal microscopy, and quartz crystal microbalance with dissipation monitoring. We find that lactoferrin binds to mucin at its native state, heat-treatment at 95 °C enhances its affinity for mucin, and that the adhesion mechanism relies on hydrophobic interactions with no obvious contributions of disulfide bonds. Lactoferrin and its resulting complexes with mucin present high surface activity, which induces an artificial shear-thinning rheological response. While electrostatic interactions have been considered the dominant mucoadhesive mechanism of native lactoferrin up to now, our findings highlight the role of hydrophobic interactions, providing a design route to alter the structural state of the protein to inspire the development of future natural protein-based mucoadhesive systems.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145311921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rational Designing of Hypocrellin B-Loaded Mesoporous Silicia-NaYF₄@NaSmF₄ Core@Shell Upconversion Nanoparticles for Targeted In Vitro Breast Cancer Therapy.","authors":"Selvamuthu Preethi, Ponnusamy Chandrasekar, Darson Jaison, Krishnaswami Venkateshwaran, Arunachalam Arulraj, Alagarsamy Shanmugarathinam, Natesan Subramanian","doi":"10.1021/acsabm.5c01524","DOIUrl":"https://doi.org/10.1021/acsabm.5c01524","url":null,"abstract":"<p><p>Breast cancer, the second leading cause of death among women globally, presents challenges to conventional treatments. To combat this, researchers are more interested in developing minimally invasive alternative treatments and have emerged with a potential photodynamic treatment (PDT). To enhance the PDT efficacy and explore its potential in biological applications, materials such as upconversion nanoparticles (UCNPs) are being utilized. In this context, a combination of mesoporous silica core-shell UCNPs (mSi-CS-UCNPs: mSi-NaYF₄@NaSmF₄) loaded with Hypocrellin B (HB) was successfully synthesized via a layer-by-layer method (hydrothermal and coprecipitation methods). The structural and morphological studies of the synthesized HB-loaded mSi-CS-UCNPs were performed by XRD, FESEM, and HRTEM. The HB content was determined by RP-HPLC with peak detection at 254 nm and 3.8 min retention time. The in vitro release profile for HB, α-, and αβ-HB-mSi-CS-UCNPs gives 4.87, 38.9, and 56.47% of HB release at 72 h, respectively. The α and αβ phases of HB-mSi-CS-UCNPs under 980 nm near-infrared (NIR) light irradiation show 39 and 42% cell viability with a 29 μg/mL IC₅₀ value on 24 h treatment, which is less compared to that of the blank and HB-mSi-CS-UCNPs without light irradiation. Consequently, the reactive oxygen species (ROS) assay, cell apoptosis assay, and mitochondrial membrane potential (ΔΨ_m) of HB-loaded mSi-CS-UCNPs-treated MCF-7 breast cancer cells under 980 nm light irradiation also show better results, which represent higher singlet oxygen (¹O₂) production, leading to late apoptotic cells and features a decrease of ΔΨ_m by ROS generation. These results indicate that the HB-mSi-CS-UCNPs can effectively activate the loaded photosensitizers in the presence of 980 nm NIR light irradiation and produce ¹O₂ for cancer cell death by either apoptosis or necrosis and could be used as a therapeutic modality for the treatment of breast cancers.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Phototriggered and pH-Responsive Micellar Cancer Drug Delivery System with Real-Time Monitoring through the NSET Mechanism.","authors":"Shalini Dyagala, Milan Paul, Sneha Das, Sayantan Halder, Swati Biswas, Subit K Saha","doi":"10.1021/acsabm.5c00770","DOIUrl":"https://doi.org/10.1021/acsabm.5c00770","url":null,"abstract":"<p><p>A phototriggered and pH-responsive cancer drug delivery system based on polymeric micelles was formulated, utilizing easily available and cost-effective materials such as an amphiphilic diblock copolymer (mPEG-PLA) and a Spiropyran derivative. It addressed the major challenges in drug delivery systems, i.e., monitoring real-time drug release, targeted, and on-demand drug delivery. To monitor real-time drug release, the fluorescence quenching of the cancer drug, doxorubicin (DOX), by <i>in situ</i> synthesized gold nanoparticles (AuNPs) through the Nanomaterial Surface Energy Transfer (NSET) mechanism was explored. Photoisomerization and size switching were characterized using UV-vis spectroscopy and dynamic light scattering (DLS) techniques. The NSET process during <i>in situ</i> synthesis of AuNPs and drug release from nanocarrier after 365 nm UV light exposure was demonstrated by steady-state and time-resolved fluorescence of DOX. The mPEG-PLA-Spiropyran-DOX (3:1:1) formulation exhibited ∼ 73.16% encapsulation efficiency and ∼ 6.45% DOX-loading with proven kinetic stability. Sustained DOX release over 50 h was validated through <i>in vitro</i> studies at pH 5.5, 6.5, and 7.4, showing enhanced DOX release at acidic pH 5.5, representative of cancer cell organelles with prolonged UV exposure. Cell internalization, intracellular phototriggered drug release, and fluorescence cell imaging in mouse breast adenocarcinoma cells (4T1) were investigated. The results demonstrated that the micellar nanocarrier, after 365 nm UV light exposure, was highly efficient in inducing apoptosis, significant cytotoxicity, and mitochondrial membrane depolarization. Furthermore, <i>in vivo</i> studies were conducted in both oral and breast cancer-bearing mice to assess tumor growth inhibition, changes in body weight, tumor weight, and immunohistochemical analysis. Notably, the therapeutic response was more pronounced in oral cancer, allowing for enhanced UV penetration and efficient activation of the micellar system. Overall, this study highlighted the potential of the dual-responsive micellar drug delivery system for targeted, on-demand cancer therapy with real-time monitoring. The enhanced efficacy in treating superficial malignancies underscores its promise for future clinical applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anisotropic Tussah Silk Nanofiber Scaffolds for Accelerating Wound Repair.","authors":"Yi Luo, Na Li, Jie Tu, Pan Shen, Yingchao Shen, Shijun Lu, Feng Zhang","doi":"10.1021/acsabm.5c01467","DOIUrl":"https://doi.org/10.1021/acsabm.5c01467","url":null,"abstract":"<p><p>Scaffolds with anisotropic structures are attractive for wound repair due to their enhanced ability to promote cell adhesion, migration, and tissue growth. In this study, anisotropic tussah silk nanofiber (TSn) scaffolds were developed through physical shearing, NaOH hydrolysis, and ice templating to accelerate wound healing. The anisotropic TSn scaffolds demonstrated radial and aligned structures, which would influence cell behavior and tissue regeneration. In vitro cellular studies have demonstrated that radial and aligned structures promoted fibroblast proliferation and directional migration. Furthermore, the disordered scaffolds (DS), aligned scaffolds (AS), and radial scaffolds (RS) were investigated to elucidate the effect of the different structures on wound healing. In vivo results demonstrated that the RS (99.8 ± 0.1%) exhibited the fastest healing rate, which may be attributed to the enhanced peripheral to central growth of blood vessels (the positive expression rates of CD31 and α-SMA were 13.0 ± 1.1% and 18.8 ± 0.8%, respectively) and tissues (the collagen deposition rate was 71.2 ± 3.5%) 14 days post implantation under the guidance of its radial structure. Taken together, biomimetic TSn scaffolds with radial structures performed the best in wound healing, offering promising applications in skin wound healing.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skin Adhesive 3D-Printable BSA-Amyloid/Cellulose Hybrid Hydrogel Film for Rapid Wound Healing and Skin Regeneration with Enhanced Antioxidant and Anti-Inflammatory Properties.","authors":"Saurabh Kumar Srivastava, Shikha Tripathi, Sakshi Agarwal, Rahul Ranjan, Somesh Agrawal, Prodyut Dhar, Eugene B Postnikov, Shilpi Chaudhary, Vinod Tiwari, Avanish Singh Parmar","doi":"10.1021/acsabm.5c01216","DOIUrl":"https://doi.org/10.1021/acsabm.5c01216","url":null,"abstract":"<p><p>Natural polymer-based hydrogels closely mimic the extracellular matrix, making them ideal for supporting cell growth and tissue regeneration. Recent advancements in tuning their porosity, morphology, and size have helped overcome key challenges in tissue engineering, such as vascularization and multicellular integration. However, their clinical use is often limited by drawbacks, such as low mechanical strength, structural instability, high production costs, and limited reproducibility. In this work, we present a skin-adhesive, 3D-printable/injectable hybrid hydrogel composed of natural protein and cellulose. This hybrid hydrogel overcomes the limitations of conventional systems by enhancing mechanical strength, scaffold stability, reproducibility, cost-effectiveness, and adhesive properties while preserving high biocompatibility and biodegradability. Using the same formulation, a wound dressing material is fabricated and applied at the wound site either by suturing or as an adhesive film. Furthermore, the hydrogel exhibits inherent antibacterial, antioxidant (60% of radical scavenging), anti-inflammatory, cell viability (up to 90%), and cell migration properties that significantly promote wound healing. This multifunctional hybrid hydrogel offers a promising solution for next-generation wound dressing applications and contributes to the advancement of bioactive and customizable materials in regenerative medicine.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellulose-Silver and Cellulose-Gold Bioactive Nanocomposites Obtained Using SCOBY Purified Membranes.","authors":"Violeta Dediu, Mariana Buşilă, Claudia Ungureanu, Leontina Grigore-Gurgu, Mihaela Cotârleṭ, Cosmin Romanitan, Vasilica Tucureanu, Oana Brincoveanu, Bogdan Stefan Vasile, Gabriela Elena Bahrim","doi":"10.1021/acsabm.5c00395","DOIUrl":"https://doi.org/10.1021/acsabm.5c00395","url":null,"abstract":"<p><p>The green synthesis of bioactive nanomaterials is becoming more attractive in various fields like biotechnology, pharmaceuticals, cosmeceuticals, etc. In this study, bacterial cellulose-silver and bacterial cellulose-gold bionanocomposites were obtained through an environmentally friendly and low-cost method without using additional reducing agents. In the first step, the bacterial cellulose, a byproduct from kombucha production using a symbiotic culture of bacteria and yeast (SCOBY), was purified using an alkaline solution. In the second step, the purified bacterial cellulose (SBC) was used to obtain silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) on the bacterial cellulose matrix, starting only from metal precursors in different media: water, black tea, and kombucha. Gold and silver nanoparticles were obtained on cellulose fibrils in all media, even in water, indicating the reducing role of cellulose. The morphology and structural features of the noble metal nanoparticles/bacterial cellulose nanocomposites (AgNPs/SBC and AuNPs/SBC) were investigated. Scanning electron microscopy (SEM) images show nanoparticles with an irregular shape with dimensions ranging from a few nanometers up to 70 nm, depending on the synthesis medium. TEM analysis revealed mostly quasi-spherical nanoparticles distributed along the surface of the cellulose fibers or within the interfibrillar pores. All nanoparticles are well crystallized and generally formed from more than two nanocrystallites, except AuNPs obtained in kombucha which are monocrystalline. XRD analysis shows the characteristic diffractograms of Iβ cellulose allomorphs and confirms the formation of crystalline AgNPs and AuNPs. The antioxidant capacity tests determined that the best activity was registered for the AgNPs/SBC composites obtained in kombucha and fresh black tea. The antimicrobial potential was evaluated against the Gram-negative bacteria <i>Escherichia coli</i> and the Gram-positive bacteria <i>Staphylococcus aureus</i> and <i>Listeria monocytogenes</i>. Cellulose-silver and cellulose-gold nanocomposites showed increased antimicrobial activity compared with raw SBC, especially in the case of kombucha medium for green synthesis. The highest antioxidant activity, determined by DPPH and ABTS assays, was obtained for AgNPs/SBC produced in kombucha and fresh black tea. Based on the results, cellulose-silver and cellulose-gold nanocomposites could be considered as bioactive materials for multiple practical applications, such as the medical field and food packaging.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Mesenchymal Stem Cell Derived Exosomes Endowed with miR-13474 as a Therapeutic Delivery Vehicle for Diabetic Wound Healing by Targeting the CPEB2/TWIST1 Axis.","authors":"Hui Shi, Xinye Han, Yuting Lu, Yingzhe Li, Hanqiang Lu, Hui Qian, Wenrong Xu","doi":"10.1021/acsabm.5c01352","DOIUrl":"https://doi.org/10.1021/acsabm.5c01352","url":null,"abstract":"<p><p>A delayed healing process in diabetic wounds is intractable. In this study, a high-glucose condition was found to be responsible for skin structure destruction, inflammatory infiltration, and vital cell dysfunction. Extracellular vesicles, particularly exosomes secreted by hucMSCs, contribute to improved diabetic wound healing, largely by promoting tissue repair and re-establishing normal function in affected cells. Small RNA-sequencing revealed that hucMSC-derived exosomes (hucMSC-Ex) were highly enriched in NC_000019.10_13474 (miR-13474), which was predicted to be an miRNA with an undiscovered function. miR-13474 showed a reduced expression level in high-glucose-treated skin cells as well as diabetic foot ulcer (DFU) rats. Moreover, there is also a significant expression difference between the wound area and the wound edge in DFU patients, indicating the potential clinical value of miR-13474. Blocking miR-13474 in hucMSC-Ex obviously diminished the therapeutic effects. Furthermore, exosomal miR-13474 was found to target the CPEB2/TWIST1 axis to improve the impaired function of skin cells. On this basis, hucMSC-Ex were used as a vehicle for the delivery of therapeutic miR-13474 to optimize the repairing effect. The study has revealed the role of hucMSC-derived exosomes and the underlying molecular mechanism in diabetic wound healing and proposes a cell-free-based modification strategy for refractory wound management.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theranostic Potential of Metal-Organic Framework/Polydopamine-Modified Magnetic Nanoparticles for Photothermal/Chemotherapy and MR Imaging.","authors":"Elnaz Mohammadi, Fatemehsadat Pishbin, Azadeh Ghaee, Mohammad Akrami, Fatemeh Rezaei","doi":"10.1021/acsabm.5c00702","DOIUrl":"https://doi.org/10.1021/acsabm.5c00702","url":null,"abstract":"<p><p>Theranostic nanoparticles that integrate therapeutic and diagnostic functions in one system offer significant potential for cancer treatment. The present study aimed to develop a theranostic hybrid nanosystem based on cobalt ferrite (CFO), polydopamine (PDA), and UiO-66-NH₂ (UiO) metal organic framework, to be used for magnetic resonance imaging (MRI) applications and synergistic photothermal therapy (PTT)/chemotherapy. Material characterizations confirmed that a nanostructure with a mean diameter of less than 100 nm has been successfully constructed. Due to the CFO component, the CFO@PDA@UiO demonstrated a relaxivity of 101.3 mM^-1. s^-1, showing potential as a T₂-weighted contrast agent. PDA improved the photothermal conversion capability of the CFO@PDA@UiO hybrid nanosystem, increasing the medium temperature within the hyperthermia therapy range. The UiO shell also acted as a carrier for the anticancer drug Gemcitabine (GEM). Ultraviolet-visible (UV-vis) spectroscopy analysis estimated that nanoparticles have a loading capacity of about 17.59% and approximately 30% of GEM was released at acidic pH (5.5). Drug-loaded nanoparticles demonstrated excellent hemocompatibility, with less than 5% hemolysis at concentrations up to 500 μg/mL. Compared to the free GEM, CFO@PDA@UiO-GEM exhibited more effective anticancer activity against MCF-7 cancer cells under NIR laser irradiation. As a result, the synthesized CFO@PDA@UiO hybrid nanosystem showed great potential as a candidate for cancer theranostic applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photothermal Micro-Nano Solid Substrates for Intracellular Delivery via Laser-Assisted Photoporation.","authors":"Jairo David Narro Silva, Prathamesh Sarnaik, Carlos Eduardo Fajardo Barocio, Vianni Chopra, Gaurav Chauhan","doi":"10.1021/acsabm.5c00742","DOIUrl":"https://doi.org/10.1021/acsabm.5c00742","url":null,"abstract":"<p><p>Introducing functional molecules into cells relies on carrier- or membrane disruption-based methods. Photoporation, utilizing laser irradiation to create transient membrane pores, facilitates the cytoplasmic entry of exogenous molecules. Photothermal agents, such as nanoparticles, bind to cell membranes and induce localized damage via thermal conversion or vapor nanobubbles. Vapor nanobubbles form thermally or via multiphoton ionization. Photothermal substrates, with immobilized agents, reduce cytotoxicity and serve as biocompatible cell culture platforms. Cell morphology and cytoskeletal organization significantly influence the efficacy of membrane disruption. Micronanopatterned surfaces control focal adhesion spatial organization and cytoskeleton arrangement. Engineered photothermal agents regulate cell spreading and adhesion, enhancing membrane disruption or resealing. In the context of intracellular delivery, this review examines the interactions among membrane mechanics, cytoskeletal structure, and photothermal agents. Recent developments in photothermal substrates, the effect of cell shape on membrane rupture, and current approaches to designing photothermal platforms to increase delivery efficiency are also covered. Understanding these mechanisms advances intracellular delivery strategies, improving targeted molecule delivery efficiency and minimizing cytotoxic effects. Further exploration of the interplay between the cell structure and disruption mechanisms is warranted for optimizing intracellular delivery techniques.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}