ACS Applied Bio Materials最新文献

{"title":"Multifunctional Dual Enzyme-Responsive Nanostructured Lipid Carriers for Targeting and Enhancing the Treatment of Bacterial Infections.","authors":"Kerisha Chetty, Xylia Q Peters, Calvin A Omolo, Eman A Ismail, Mohammed A Gafar, Eman Elhassan, Sania Z F Kassam, Jasoda Govender, Sbongumusa Dlamini, Thirumala Govender","doi":"10.1021/acsabm.4c01436","DOIUrl":"https://doi.org/10.1021/acsabm.4c01436","url":null,"abstract":"<p><p>Bacterial infections pose an increasingly worrisome threat to the health of humankind, with antibiotic resistance contributing significantly to this burden. With current conventional antibiotics perpetuating the problem, and a paucity in developing antibiotics, drug delivery systems incorporating nanotechnology appear promising. As such, a dual enzyme-responsive multifunctional nanostructured lipid carrier (NLC) incorporating farnesol (FAN) and triglycerol monostearate (TGMS), was conceptualized for the codelivery of vancomycin (VCM) and antimicrobial peptide (AMP) to enhance the antibacterial activity of VCM. In silico studies and Microscale Thermophoresis demonstrated the strong binding relationships between the NLC constituents and two enzymes that exist in higher concentrations during host infection, namely lipase and a matrix metalloproteinase (MMP). The formulated nanosystem, VCM-AMP-TF-NLCs, had a particle size, polydispersity index, zeta potential, and entrapment efficiency of 149.00 ± 2.97 nm, 0.07 ± 0.01, -5.51 ± 1.21 mV, and 86.20% ± 1.47%, respectively. The NLCs, which showed stability, and biocompatibility, also demonstrated lipase- and MMP-responsiveness. The in vitro antibacterial studies revealed 2-fold and 8-fold reductions in the minimum inhibitory concentration for the NLCs compared to bare VCM, against methicillin-resistant <i>Staphylococcal aureus</i> (MRSA) and <i><i>Escherichia coli</i></i>, respectively. Furthermore, in vivo studies revealed that tissues treated with the VCM-AMP-TF-NLCs displayed significantly reduced bacterial burdens (up to 8.73-fold) and less histopathological cellular injury, edema, and necrosis compared to the tissues treated with bare VCM alone. The results support the superiority of the VCM-AMP-TF-NLCs as a multifunctional dual enzyme-responsive NLC compared to bare VCM.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Casting Skin Dressing Containing Extractions of the Organic Part of Marine Sponges for Wound Healing.","authors":"Amanda de Souza, Cintia C S Martignago, Lívia Assis, Fernanda Vieira Botelho Delpupo, Marcelo Assis, Karolyne S J Sousa, Lais Caroline Souza E Silva, Laura O Líbero, Flavia de Oliveira, Ana Claudia Muniz Renno","doi":"10.1021/acsabm.4c01497","DOIUrl":"https://doi.org/10.1021/acsabm.4c01497","url":null,"abstract":"<p><p>Skin wounds are extremely frequent injuries related to many etiologies. They are a burden on healthcare systems worldwide. Skin dressings are the most popular therapy, and collagen is the most commonly used biomaterial, although new sources of collagen have been studied, especially spongin-like from marine sponges (SPG), as a promising source due to a similar composition to vertebrates and the ability to function as a cell-matrix adhesion framework. Despite evidence showing the positive effects of SPG for tissue healing, the effects of skin dressings manufactured are still limited. In this context, this study aimed at investigating the effects of collagen skin dressings in an experimental model of skin wounds in rats. For this purpose, SEM, FTIR, cell viability, morphological and morphometric aspects, collagen deposition, and immunostaining of TGF-β and FGF were evaluated. The results demonstrated micro- and macropores on the rough surface, peak characteristics of collagen, and no cytotoxicity for the skin dressing. Also, the control group (CG) after 5 and 10 days exhibited an intense inflammatory process and the presence of granulation tissue, while the treated group (TG) exhibited re-epithelialization after 10 days. The evaluation of granulation tissue and neoepithelial length had an intragroup statistical difference (<i>p</i> = 0.0216) and no intergroup difference. Birefringence demonstrated an organized mesh arranged in a network pattern, presenting type I and type III collagen fibers in all groups. Moreover, in the morphometric evaluation, there were no statistical differences in intergroups or time points for the different types of collagen evaluated. In conclusion, these findings may indicate that the dressing has not exacerbated the inflammatory process and may allow faster healing. However, further studies using a critical wound healing injury model should be used, associated with longer experimental periods of evaluation, to further investigate the effects of these promising therapeutic approaches throughout the skin repair process.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Montmorillonite-Based Pickering Nanoemulsion for the Integration of Photothermal Therapy and NIR-Responsive Drug Delivery.","authors":"Yonghui Zan, Jiaren Liu, Ziwei Zhao, Yi Wei, Ning Yang, Hean Zhang, Xiaoyu Wang, Yuetong Kang","doi":"10.1021/acsabm.4c01501","DOIUrl":"https://doi.org/10.1021/acsabm.4c01501","url":null,"abstract":"<p><p>Chemo/photothermal combination therapy is a promising and practical approach for cancer treatment which calls for certain nanovehicles to achieve the spatiotemporal co-occurrence of photothermal conversion and drug delivery. Herein, we developed a montmorillonite-based Pickering emulsion equipped with a near-infrared photothermal agent (indocyanine green, ICG) and anticarcinogen (paclitaxel, PTX). With both montmorillonite and ICG functioning as interfacial stabilizers, the Pickering emulsion showed good stability and nanoscale droplet size, which were favored for cellular applications. Due to the vast oil-water interface, where the majority of amphiphilic ICG was prone to distribute, the Pickering nanoemulsion could achieve a higher local concentration of ICG than the aqueous solution, therefore leading to a higher local photothermal performance under near-infrared irradiation. The Pickering nanoemulsion exhibited fast cell penetration, which promoted the photothermal therapeutic effect of ICG. Moreover, the inner phase of the Pickering nanoemulsion also facilitated the loading of PTX, further improving its killing efficacy against cancer cells under near-infrared irradiation, because the photothermal conversion of the Pickering nanoemulsion could not only cause heat damage by itself but also promote the loaded PTX to diffuse out and induce cell death. Therefore, this clay-based Pickering nanoemulsion as a nanovehicle could realize the synergy of chemo- and photothermal therapy.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cell-Laden Engineered Patch: Advances and Applications in Tissue Regeneration.","authors":"Seyeong Heo, Minhyeok Noh, Yeonseo Kim, Sunho Park","doi":"10.1021/acsabm.4c01427","DOIUrl":"https://doi.org/10.1021/acsabm.4c01427","url":null,"abstract":"<p><p>Stem cell-based therapies are emerging as significant approaches in tissue engineering and regenerative medicine, applicable to both fundamental scientific research and clinical practice. Despite remarkable results in clinical studies, challenges such as poor standardization of graft tissues, limited sources, and reduced functionality have hindered the effectiveness of these therapies. In this review, we summarize the engineering approaches involved in fabricating stem cell assisted patches and the substantial strategies for designing stem cell-laden engineered patches (SCP) to complement the existing stem cell-based therapies. We then outline the potential applications of SCP in advancing tissue regeneration and regenerative medicine. By combining living stem cells with engineered patches, SCP can enhance the functions of both components, particularly for tissue engineering applications. Finally, we addressed current challenges, such as ethical considerations, high costs, and regulatory hurdles and proposed future research directions to overcome these barriers.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-Activated Near-Infrared-II Afterglow Luminescence for Precise Cancer Imaging.","authors":"Meng Yuan, Xiao Fang, Wenzheng Liu, Xiaoguang Ge, Ying Wu, Lichao Su, Shi Gao, Jibin Song","doi":"10.1021/acsabm.4c01352","DOIUrl":"https://doi.org/10.1021/acsabm.4c01352","url":null,"abstract":"<p><p>Afterglow fluorescence imaging has been extensively assessed in ultrasensitive bioimaging. Since it eliminates the need for real-time excitation light and thereby circumvents the autofluorescence background of tissue, it holds tremendous potential in accurate biomedical imaging. However, current afterglow probes are rare and emit light only in the visible to near-infrared (NIR) range, which is inadequate for <i>in vivo</i> imaging. To resolve this issue, an ultrasound (US)-activated NIR-II afterglow luminescence probe (NPs-Ce4-SN) emitting afterglow luminescence with a peak at ∼1100 nm was developed. This peak is nearly 400 nm red-shifted compared with other reported afterglow probes. Of note, after US termination, NPs-Ce4-SN undergoes energy transformation to produce ¹O₂ and subsequently undergoes internal oxidation-reduction reaction to produce NIR-II afterglow, generating high signal-to-noise ratio and high-penetration depth imaging. <i>In vitro</i> and <i>in vivo</i> NIR-II afterglow imaging experiments revealed that NPs-Ce4-SN has good biocompatibility and deep tissue penetration depth, suggesting a diagnostic strategy for <i>in vivo</i> tumor imaging with a high signal-to-noise ratio.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA-Assisted CRISPR-Cas12a Enhanced Fluorescent Assay for Protein Detection in Complicated Matrices.","authors":"Sathishkumar Munusamy, Haiyan Zheng, Rana Jahani, Shuo Zhou, Jun Chen, Juanhua Kong, Xiyun Guan","doi":"10.1021/acsabm.4c01600","DOIUrl":"https://doi.org/10.1021/acsabm.4c01600","url":null,"abstract":"<p><p>Proteins are important biological macromolecules that perform a wide variety of functions in the cell and human body, and can serve as important biomarkers for early diagnosis and prognosis of human diseases as well as monitoring the effectiveness of disease treatment. Hence, sensitive and accurate detection of proteins in human biospecimens is imperative. However, at present, there is no ideal method available for the detection of proteins in clinical samples, many of which are present at ultralow (less than 1 pM) concentrations and in complicated matrices. Herein, we report an ultrasensitive and selective DNA-assisted CRISPR-Cas12a enhanced fluorescent assay (DACEA) for protein detection with detection limits reaching as low as attomolar concentrations. The high assay sensitivity was accomplished through the combined DNA barcode amplification (by using dual-functionalized AuNPs) and CRISPR analysis, while the high selectivity and high resistance to the matrix effects of our method were accomplished via the formation of protein-antibody sandwich structure and the specific recognition of Cas12a (under the guidance of crRNA) toward the designed target ssDNA. Given its ability to accurately and sensitively detect trace amounts of proteins in complicated matrices, the DACEA protein assay platform pioneered in this work has a potential application in routine protein biomarker testing.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposomal Encapsulation of Chlorambucil with a Terpyridine-Based, Glutathione-Targeted Optical Probe Facilitates Cell Entry and Cancer Cell Death.","authors":"Mallayasamy Siva, Kiran Das, Priya Rana, Abhijit Saha, Debasish Mandal, Atanu Barik, Adele Stewart, Biswanath Maity, Priyadip Das","doi":"10.1021/acsabm.4c01448","DOIUrl":"https://doi.org/10.1021/acsabm.4c01448","url":null,"abstract":"<p><p>The nitrogen mustard alkylating agent chlorambucil (CBL) is a critical component of chemotherapeutic regimens used in the treatment of chronic lymphocytic leukemia. The cancer cell-killing actions of CBL are limited by glutathione (GSH) conjugation, a process catalyzed by the GSH transferase hGSTA1-1 that triggers CBL efflux from cells. In the cancer cell microenvironment, intracellular GSH levels are elevated to counterbalance oxidative stress generated due to the high glycolytic demand. As many chemotherapeutic drugs trigger cell death through mechanisms that depend on reactive oxygen species (ROS), antioxidant capacity in cancer cells also represents a barrier to anticancer therapies. Here, we demonstrate that a heightened GSH content in cancer cells can also be exploited for cell-selective drug delivery. We successfully synthesized a malononitrile conjugate terpyridine-based derivative <b>L1</b>, which specifically reacts with GSH in the presence of other biologically relevant amino acids including cysteine (Cys) and homocysteine (Hcy). The significant change in the electronic spectra of <b>L1</b> in the presence of GSH confirmed GSH detection, which was further corroborated by density functional theory calculations. We next encapsulated CBL into <b>L1</b>-containing, anthracene-functionalized, and 10,12-pentacosadiynoic acid (PCDA)- and 1,2-dimyristoyl-<i>sn</i>-glycero-3-phosphocholine (DMPC)-based liposomes (<b>Lip-CBL-L1</b>). We established successful CBL encapsulation and release from <b>L1</b>-containing liposomes in GSH-enriched cancer cells in vitro. Both <b>Lip-CBL-L1</b> and the <b>L1</b>-lacking <b>Lip-CBL</b> control displayed cell-killing activity. However, human triple-negative breast cancer cells MDAMB231, human lung cancer cells A549, and murine leukemic WEHI cells were more sensitive to the cytotoxic effects of <b>Lip-CBL-L1</b> compared to the nonmalignant cells (AC16 and HEK293). Indeed, in these cancer cell lines, <b>Lip-CBL-L1</b> induced greater ROS generation compared to that of <b>Lip-CBL</b>. Together, our results provide initial evidence of the feasibility of exploiting the unique oxidant environment of cancer cells for optimized drug delivery.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun 11β-HSD1 Inhibitor-Loaded Scaffolds for Accelerating Diabetic Ulcer Healing.","authors":"Xiaofeng Ding, Heyan Huang, Yutong Chen, Junchao Wu, Xin Yan, Youjun Ding, Jie Dong, Yiwei Wang, Lili Wang, Qian Tan, Chenxi Yang","doi":"10.1021/acsabm.4c01397","DOIUrl":"https://doi.org/10.1021/acsabm.4c01397","url":null,"abstract":"<p><p>Diabetic ulcers (DUs) are a common and severe complication of diabetes, characterized by impaired wound healing due to a complex pathophysiological mechanism. Elevated levels of 11β-hydroxysteroid dehydrogenase type I (11β-HSD1) in wounds have been demonstrated to modulate glucocorticoid activity, leading to delayed skin cell proliferation and restricted angiogenesis, ultimately hindering wound healing. In this study, we propose an electrospun poly(ε-caprolactone) (PCL) nanofiber scaffold doped with the 11β-HSD1 inhibitor BVT2733 (BPs) to prevent 11β-HSD1 activity during the diabetic wound healing process. The electrospun scaffold loaded with BVT2733 is designed to achieve localized inhibition of 11β-HSD1 in DUs. This scaffold exhibited a porous morphology and desirable drug-loading capacity, meeting the requirements for wound coverage and effective delivery of BVT2733 BPs. In vitro studies demonstrated that the sustained release of BVT2733 from the scaffold promoted skin cell proliferation and migration while stimulating angiogenesis by upregulating HIF1-α/VEGF expression. The therapeutic effect of the scaffold was further confirmed in a full-thickness wound model using diabetic mice. The mice treated with the scaffolds exhibited an accelerated wound healing rate, increased neovascularization, enhanced collagen deposition, and regeneration of skin appendages within 2 weeks postinjury. The findings here provide evidence for the use of 11β-HSD1 inhibitor-integrated biomaterials in treating DUs and represent a novel biological platform for modulating dysregulated mechanisms in DUs.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking Photosynthetic Potential: Harnessing <i>Rosa roxburghii</i> Derived Carbon Dots as Nanofertilizers for Enhanced Plant Growth.","authors":"Qingyun Xu, Jijie Han, Dongyu Wang, Jianle Zhuang, Chaofan Hu, Hanwu Dong, Wei Li, Bingfu Lei, Yingliang Liu","doi":"10.1021/acsabm.4c01609","DOIUrl":"https://doi.org/10.1021/acsabm.4c01609","url":null,"abstract":"<p><p>The synthesis of nanomaterials from renewable resources has emerged as an environmentally friendly alternative. This approach helps to reduce the use of chemical fertilizers in agricultural production, further reducing the potential harm to the ecosystem and effectively reducing the burden on the environment. In this work, we synthesized <i>Rosa roxburghii</i> derived carbon dots (CDs) using the microwave hydrothermal method (RR-CDs) and the electrolytic oxidation method (GRR-CDs), and the results showed that RR-CDs had a wider ultraviolet absorption range and emitted blue fluorescence. These properties make RR-CDs more effective as light-harvesting materials in plants, thus promoting photosynthesis. In the cultivation of lettuce, RR-CDs significantly enhanced both the biomass and the quality of the lettuce. In addition, compared to the control group, the chlorophyll content of lettuce treated with RR-CDs increased by 31.83%, the net photosynthetic rate increased by 60.76%, and the electron transport rate of photosystem II increased by 38.72%. Therefore, we found that the microwave hydrothermal method could bring better benefits, with a yield of up to 40.20% after just 2 h of reaction. RR-CDs promote photosynthesis by promoting light conversion and improving nutrient efficiency while also boasting the dual advantages of low cost and easy large-scale production, thus opening up avenues for sustainable agricultural production.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrafast Microwave-Synthesized 2D/1D MnO₂/Carbon Nanotube Hybrid for Bilirubin Detection in Simulated Blood Serum.","authors":"Manaswini Ravipati, Sumit Chahal, Sushmee Badhulika","doi":"10.1021/acsabm.4c00792","DOIUrl":"https://doi.org/10.1021/acsabm.4c00792","url":null,"abstract":"<p><p>Hybridization of carbon nanotubes (CNTs) and manganese dioxide (MnO₂) integrates the biocompatibility and outstanding electrocatalytic activity of MnO₂ with the exceptional conductivity of CNTs, thus providing a superior synergistic sensing platform for the detection of biomolecules. However, the existing methods for synthesizing MnO₂/CNT hybrids are complex and inefficient, resulting in low yields and limited surface functionalities. Hence, in this study, we present a low-cost and ultrafast solid-phase synthesis of the MnO₂/CNT hybrid using a facile microwave technique to detect a crucial biomolecule bilirubin. The successful synthesis of the MnO₂/CNT hybrid is confirmed through characteristic Raman and X-ray diffraction peaks, while morphology is analyzed by imaging techniques such as FESEM and HRTEM. The MnO₂/CNT/nickel foam (NF) sensor is thereafter used for the electrochemical detection of bilirubin. The sensor demonstrates a wide linear detection range from 10 nM to 1 mM, with a sensitivity of 6.87 mA nM^-1 cm^-2 toward bilirubin, as determined through the differential pulse voltammetry technique. The lower limit of detection is noted at 3.3 nM (=3.3 <i>S</i>/<i>m</i>). Furthermore, the as-fabricated sensor showcases high selectivity against the interfering species. Real-time analysis conducted in simulated blood serum using the standard addition method reveals an outstanding recovery percentage of approximately 98%. The conductive MnO₂/CNT hybrid interacts robustly with bilirubin, aided by the porous NF substrate for stability, catalytic activity, and rapid electron transfer, enabling sensitive bilirubin detection. The work provides an ultrafast, low-cost, and high-yield solid-phase microwave synthesis of MnO₂/CNT hybrid material and broadens its application in the detection of biological specimens for clinical diagnosis and biomedical research.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}