ACS Applied Bio Materials最新文献

Chitosan-Supported Molecularly Imprinted Polypyrrole@MoSe₂ Nanosheet-Based Bioinspired Electrochemical Sensor for Improved Quantification of Gut Microbiota-Derived Hippuric Acid.

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-06-02 DOI: 10.1021/acsabm.5c00329

Archana, Anil Kumar, Pratima R Solanki

{"title":"Chitosan-Supported Molecularly Imprinted Polypyrrole@MoSe2 Nanosheet-Based Bioinspired Electrochemical Sensor for Improved Quantification of Gut Microbiota-Derived Hippuric Acid.","authors":"Archana, Anil Kumar, Pratima R Solanki","doi":"10.1021/acsabm.5c00329","DOIUrl":"https://doi.org/10.1021/acsabm.5c00329","url":null,"abstract":"Hippuric acid (HA) is a well-known gut microbiota-derived metabolite involved in the development of various diseases such as chronic kidney disease, Chron's disease, and inflammatory bowel disease. HA being one of the most commonly found metabolites in gut microbiota is an important analyte considered for establishing an efficient, quick, sensitive, and reliable method for its detection in human samples. In this work, a molecularly imprinted polymer (MIP) technique has been focused on the electrochemical detection of HA using soft materials, as well as a bioinspired technique. Different characterization techniques have been used to validate the synthesis of MoSe2 NS, polypyrrole (PPY)@molybdenum diselenide (MoSe2)-MIP, such as X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, atomic force microscopy, contact angle, Brunauer-Emmett-Teller method, and transmission electron microscopy. Also, density functional theory computational study has been used to establish the theoretical interaction between the pyrrole (monomer) and HA (target analyte), as well as explore the highest occupied molecular orbital and lowest unoccupied molecular orbital interactions followed by molecular electrostatic potential and Mulliken charges. The chitosan-polypyrrole@MoSe2-MIP showed 14.92 μA log10(ng/mL)-1 cm-2 sensitivity and a low limit of detection of 0.76 ng/mL in the presence of the analyte using the differential pulse voltammetry technique. The fabricated sensor was also deployed to assess the level of HA in the spiked urine sample, further showing the recovery of 93-108%. Also, the green profile score for the PPY-MIP-based sensor is assessed using the Analytical GREEness MIP (AGREEMIP) metric tool.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Coating of Calcium Titanate and High-Entropy Alloy for Improved Mechanical and Bioactive Properties of ZTA Implants.

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-06-02 DOI: 10.1021/acsabm.5c00093

Peyman Karimi, Moein Taheri, Azita Etminan, Parnia Mohebimanesh, Majid Majd, Hamed Faraji

{"title":"Dual Coating of Calcium Titanate and High-Entropy Alloy for Improved Mechanical and Bioactive Properties of ZTA Implants.","authors":"Peyman Karimi, Moein Taheri, Azita Etminan, Parnia Mohebimanesh, Majid Majd, Hamed Faraji","doi":"10.1021/acsabm.5c00093","DOIUrl":"https://doi.org/10.1021/acsabm.5c00093","url":null,"abstract":"In this study, we developed a dual-coating approach to enhance the mechanical and bioactive properties of zirconia-toughened alumina (ZTA) bioceramics for biomedical implant applications. The method applies a two-step coating process: a calcium titanate (CaTiO3) layer on the tissue-contacting region to promote bioactivity and osseointegration, and a high-entropy alloy (HEA, Fe0.25Pt0.25Zr0.25Mg0.25) coating on the remaining surface to improve mechanical strength and durability. ZTA substrates were synthesized through high-energy ball milling and sintering at 1400 °C, yielding a baseline hardness of 2.97 GPa and an elastic modulus of 79.2 GPa, as measured by nanoindentation. Molecular dynamics (MD) simulations, performed using LAMMPS with a hybrid Embedded Atom Method, Buckingham, and Lennard-Jones potential framework, produced a hardness range of 2.49-2.95 GPa, consistent with experimental results. The HEA coating increased the composite hardness to 2.63 GPa, reduced principal stresses from 1330 to 546 MPa, and improved thermal conductivity with a z-axis heat flux of 80 eV/fs·Å2, minimizing localized overheating. The CaTiO3 coating slightly increased hardness to 2.49 GPa while enhancing bioactivity through improved apatite formation and cell proliferation. Stress and thermal analyses confirmed the HEA's mechanical stability and the CaTiO3's contribution to biological integration. This dual-coating method addresses ZTA's challenges, including low-temperature degradation and limited bone bonding, providing a framework for high-performance, biocompatible dental implants.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum. 德国亲密伴侣暴力行为的发生率和预测因素：关于女性和男性受害情况的首次全国性研究。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-06-01 Epub Date: 2022-10-03 DOI: 10.1177/10497315221113884

引用次数: 0

Antimicrobial Peptide P-113-DPS Suppresses the Cariogenic Virulence of Streptococcus mutans.

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-06-01 DOI: 10.1021/acsabm.5c00314

Qing Liu, Li Zhou, Simin Peng, Quan Li Li, Hai Ming Wong

{"title":"Antimicrobial Peptide P-113-DPS Suppresses the Cariogenic Virulence of Streptococcus mutans.","authors":"Qing Liu, Li Zhou, Simin Peng, Quan Li Li, Hai Ming Wong","doi":"10.1021/acsabm.5c00314","DOIUrl":"https://doi.org/10.1021/acsabm.5c00314","url":null,"abstract":"Dental caries is a widespread and contagious chronic infectious condition. As the principal cariogenic bacterium involved in dental caries, Streptococcus mutans (S. mutans) possesses cariogenicity-related properties, including acidogenicity, aciduricity, and exopolysaccharide (EPS) synthesis. Our previously designed peptide, P-113-DPS, has demonstrated antibacterial effects on S. mutans; however, its detailed impact on its cariogenic virulence factors remains unclear. This study focused on assessing changes in these factors following treatment with P-113-DPS. Furthermore, it aimed to investigate alterations in virulence-associated gene expression in vitro. The basic viability of S. mutans after P-113-DPS treatment was evaluated using a growth curve assay and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide staining assay. Acidogenicity was assessed through monitoring pH drop and lactate dehydrogenase activity, while aciduricity was evaluated through measuring survival rates in a lethal acidic environment. Additionally, EPS synthesis was analyzed using the anthrone sulfuric acid method, and structural observations were performed with confocal laser scanning and scanning electron microscopy. Finally, the changes in gene expression were examined utilizing quantitative real-time PCR (qPCR). P-113-DPS inhibited the growth and cell viability of S. mutans. Treatment with P-113-DPS resulted in decreased acidogenicity and aciduricity, evidenced by reduced acid production and survival rates at pH 5.0. Additionally, P-113-DPS suppressed the biofilm formation and EPS synthesis. Moreover, qPCR analysis revealed that P-113-DPS downregulated the expression of S. mutans virulence-associated genes. In conclusion, P-113-DPS exhibited strong antimicrobial properties and effectively suppressed the cariogenic virulence traits of S. mutans in vitro by downregulating virulence-associated genes, highlighting its promising anticaries potential.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast Lithium-Releasing Phosphate Glasses Promote the Resolution of Inflammation. 快速释放锂磷酸盐玻璃促进炎症的消退。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-05-31 DOI: 10.1021/acsabm.5c00128

Gabriela L Abe, Linghao Xiao, Jun-Ichi Sasaki, Hirohiko Sakai, Haruaki Kitagawa, Tomoki Kohno, Naoya Funayama, Satoshi Imazato

引用次数: 0

Selective Sensing of Human Serum Albumin by a D···π···A-Based Self-Assembled Nanoprobe via a Disassembly Mechanism: Quantification and Live Cell Imaging Applications. 基于D···π····的自组装纳米探针通过拆卸机制选择性检测人血清白蛋白：定量和活细胞成像应用。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-05-31 DOI: 10.1021/acsabm.5c00650

Dolan Moni, Mihir Sasmal, Atul Katarkar, Abu Saleh Musha Islam, Mansur Habibullah, Anamika Basu, Mahammad Ali

{"title":"Selective Sensing of Human Serum Albumin by a D···π···A-Based Self-Assembled Nanoprobe via a Disassembly Mechanism: Quantification and Live Cell Imaging Applications.","authors":"Dolan Moni, Mihir Sasmal, Atul Katarkar, Abu Saleh Musha Islam, Mansur Habibullah, Anamika Basu, Mahammad Ali","doi":"10.1021/acsabm.5c00650","DOIUrl":"https://doi.org/10.1021/acsabm.5c00650","url":null,"abstract":"Human serum albumin (HSA) serves as a vital biomarker for diagnosing kidney and liver diseases. Herein, we describe the detailed synthetic procedure of a D···π···A-based fluorescent probe HPQM [(E)-2-(2-(8-(3-hydroxypropoxy)-2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-yl)vinyl)-1,1,3-trimethyl-1H-benzo[e]indol-3-ium], which forms weakly fluorescent nanoaggregates in aqueous environments due to the aggregation-caused quenching phenomenon. Nevertheless, in the presence of HSA, it becomes highly fluorescent (∼25-fold enhancement), facilitating the disassembly of nanoaggregates into its monomer. The study reveals that noncovalent interactions between HPQM and HSA promote the disassembly of the nanoaggregates and trapping of the HPQM monomer in the HSA cavity, resulting in red fluorescence by prohibiting the free rotation of the probe molecules owing to the substantial steric hindrance and low polarity environment present inside the HSA cavity. Multiple circular dichroism spectral analyses of HSA in the absence and presence of the probe revealed HPQM-induced conformational changes in the secondary and tertiary structures of HSA. The 3σ/slope technique yielded a limit of detection value of 29.8 nM (1.98 mg/L) in aqueous solutions, highlighting its high sensitivity toward HSA. Also, HPQM offers some practical utility including live cell imaging of HSA and the detection and estimation of HSA content in human blood and urine.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moisture-Absorbing and Antimicrobial Semi-Interpenetrating Polymer Network Hydrogels: An Advanced Medical and Healthcare Material. 吸湿抗菌半互穿聚合物网络水凝胶：一种先进的医疗保健材料。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-05-31 DOI: 10.1021/acsabm.5c00586

Ye Xu, Lifang Ma, Yongyu Zha, Jiangzhen Guo, Yubo Fan, Chunjing Tao

{"title":"Moisture-Absorbing and Antimicrobial Semi-Interpenetrating Polymer Network Hydrogels: An Advanced Medical and Healthcare Material.","authors":"Ye Xu, Lifang Ma, Yongyu Zha, Jiangzhen Guo, Yubo Fan, Chunjing Tao","doi":"10.1021/acsabm.5c00586","DOIUrl":"https://doi.org/10.1021/acsabm.5c00586","url":null,"abstract":"The moisture absorption and antibacterial properties of medical materials are crucial for their clinical efficacy. However, existing hydrogel materials primarily focus on enhancing single properties, leading to issues, such as poor mechanical strength and limited environmental adaptability. Therefore, the development of medical materials with the dual functions of moisture absorption and antibacterial activity is of significant importance. In this study, inspired by biomimetic multilevel porous design, a semi-interpenetrating hydrogel material (CNSL) was successfully developed by incorporating moisture-absorbing component (LiCl) and antibacterial nanoparticles (SDP-NPs) into carboxymethyl cellulose (CMC) and N-isopropylacrylamide (NIPAm) matrix. The multilevel porous structure mimics the hierarchical pore characteristics of biological systems, optimizing the specific surface area and significantly improving the moisture absorption performance in the humidity range of 15-90% (0.613-5.127 g/g). The temperature-sensitive network enables intelligent water adsorption-desorption cycling. SDP-NPs provide long-lasting antibacterial effects through sustained release within the pores and physical barriers, demonstrating bactericidal rates of 99.77 and 99.83% against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), respectively, in vitro. The CNSL hydrogel effectively achieves humidity regulation and broad-spectrum antibacterial performance, offering a unique design for the development of multifunctional medical dressings and other healthcare products. This research holds significant value in enhancing the infection prevention and control efficacy as well as clinical safety of medical materials and devices.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Bioelectronic Immunosensing Platform Comprising a Gold Dendritic 3D Architecture Sandwiched between Graphene Sheets for Oral Cancer Detection. 一种生物电子免疫传感平台，包括夹在石墨烯片之间的金树突状3D结构，用于口腔癌检测。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-05-30 DOI: 10.1021/acsabm.5c00368

Buddhadev Purohit, Rohini Kumari, Ananya Srivastava, Pranjal Chandra

{"title":"A Bioelectronic Immunosensing Platform Comprising a Gold Dendritic 3D Architecture Sandwiched between Graphene Sheets for Oral Cancer Detection.","authors":"Buddhadev Purohit, Rohini Kumari, Ananya Srivastava, Pranjal Chandra","doi":"10.1021/acsabm.5c00368","DOIUrl":"https://doi.org/10.1021/acsabm.5c00368","url":null,"abstract":"Epidemiological studies have shown that oral cancer is one of the most lethal forms of cancer, with a high risk of distant metastases and eventual death, largely due to late diagnosis. The noninvasive diagnosis of oral cancer can simplify sample collection, and when paired with single-step detection assays, it can facilitate and popularize early diagnosis, ultimately reducing the mortality rate. In light of this, we have designed a simple and noninvasive immunosensor for the facile and selective identification of the oral cancer biomarker cytokeratin 19 fragment-21-1 (CYFRA-21-1) in saliva samples, specifically in the clinically safe as well as diseased range. Prior to being utilized as a marker for oral carcinomas, CYFRA-21-1 was used as a biomarker for nonsmall cell lung cancer (NSCLC) as well as other cancers. Due to its high salivary secretion, it has become a powerful and reliable marker for the early detection of oral cancer in recent decades; however, there are very few studies pertaining to it. Moreover, the literature has reported the higher sensitivity and specificity of CYFRA-21-1 markers in saliva than in serum samples. Hence, in this study, the sensing surface was developed using a nanocomposite of gold nanodendrites (AuNDs) sandwiched between graphene oxide (GO) sheets for the sensitive detection of CYFRA-21-1 in saliva samples. Metallic dendrites based on nanohybrid systems possess astonishing conductive and catalytic properties. The first layer of GO is used as the substrate for enhanced growth of AuNDs, while the second layer of GO is utilized for covalent binding of the anti-CYFRA-21-1 antibody through bioconjugation reactions. The developed bioelectronic immunosensing platform is able to sense CYFRA-21-1 in a clinically safe as well as diseased range with a low limit of detection (LOD). It is easy to operate and can be integrated with a portable electrochemical analyzer for point-of-care (POC) oral cancer diagnosis in low-resource settings.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclosporine A-Loaded Poly(ethylene glycol)-block-poly(lactic-co-glycolic acid)-Based Mucopenetrating Nanoparticles for Treatment of Inflammatory Bowel Diseases. 负载环孢素a -聚乙二醇-块聚乳酸-共羟基乙酸基粘膜穿透纳米颗粒治疗炎症性肠病

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-05-30 DOI: 10.1021/acsabm.5c00439

Hideyuki Sato, Yuki Inoue, Taiki Adachi, Takahiro Mizumoto, Kohei Yamada, Satomi Onoue

{"title":"Cyclosporine A-Loaded Poly(ethylene glycol)-block-poly(lactic-co-glycolic acid)-Based Mucopenetrating Nanoparticles for Treatment of Inflammatory Bowel Diseases.","authors":"Hideyuki Sato, Yuki Inoue, Taiki Adachi, Takahiro Mizumoto, Kohei Yamada, Satomi Onoue","doi":"10.1021/acsabm.5c00439","DOIUrl":"https://doi.org/10.1021/acsabm.5c00439","url":null,"abstract":"This study aimed to develop cyclosporine A (CsA)-loaded mucopenetrating nanoparticles (MNP) for the safe and effective treatment of inflammatory bowel diseases (IBD). Poly(ethylene glycol)-block-poly(lactic-co-glycolic acid) (PEG-PLGA) was selected as the mucopenetrating carrier polymer for CsA/MNP to achieve topical accumulation by passive targeting and prolonged pharmacological action at the inflammation site, potentially maximizing anti-inflammatory effects with minimizing systemic exposure. Uniform nanoparticles were successfully prepared using a flash nanoprecipitation technique, and their size and dispersibility varied based on the length of the PEG chain and mixing ratio of PEG-PLGA and PLGA. The composition of CsA/MNP was optimized in terms of the dispersibility and release properties. CsA/MNP exhibited a 3-fold higher penetrating amount through artificial mucus than the reference PLGA nanoparticles. In a chemical-induced rat model of IBD, CsA/MNP accumulated at the inflamed colon site, achieving a 4-fold higher concentration of CsA than the reference PLGA nanoparticles. After repeated oral administration of CsA/MNP, significant anti-inflammatory effects were observed in the model rats compared to the reference formulation as evidenced by 73% reduction of myeloperoxidase activity in the colon tissue of the IBD model animal. Systemic exposure of CsA after a single oral administration of CsA/MNP was 25% of that after oral administration at the toxic dose of CsA. Even after repeated oral administration, no significant changes were observed in plasma biomarkers for kidney functions. PEG-PLGA-based nanoparticles may be viable oral drug delivery carriers to enhance the therapeutic potential of CsA with reduced risk of systemic side effects.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen-Bond-Assisted Dual-Mode Ratiometric Detection of Uric Acid Using Pyrimidine-Driven Charge Transfer Probes: From Mechanistic Studies to Real-Life Applications. 氢键辅助双模式比例检测尿酸使用嘧啶驱动电荷转移探针：从机理研究到现实生活中的应用。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-05-30 DOI: 10.1021/acsabm.5c00038

Animesh Pal, Imran Ahmad, Nilanjan Dey

{"title":"Hydrogen-Bond-Assisted Dual-Mode Ratiometric Detection of Uric Acid Using Pyrimidine-Driven Charge Transfer Probes: From Mechanistic Studies to Real-Life Applications.","authors":"Animesh Pal, Imran Ahmad, Nilanjan Dey","doi":"10.1021/acsabm.5c00038","DOIUrl":"https://doi.org/10.1021/acsabm.5c00038","url":null,"abstract":"The study highlights the design, synthesis, and photophysical evaluation of two pyrimidine-based charge transfer probes (compounds 1 and 2) for the selective detection of uric acid (UA) in aqueous environments and biological fluids. Both probes exhibited distinct spectral responses attributable to their structural differences, with compound 1 demonstrating superior sensitivity. Quantitative analysis revealed a strong linear correlation (R2 = 0.998) between UA concentration and fluorescence intensity, enabling detection as low as 0.12 μM, which is well within the clinically relevant range. Mechanistic investigations confirmed the indispensable role of the pyrimidine unit in forming multipoint hydrogen-bonding interactions with UA, which induced spectroscopic changes and disrupted probe aggregates. Environmental factors such as pH, polarity, and temperature were systematically examined, revealing that probe performance was optimal at moderate pH (4-8) and 60-70% water content, with elevated temperatures (50 °C) enhancing the response due to the dissociation of supramolecular aggregates. Screening in diluted human urine validated the practical applicability of the probe, demonstrating high recovery rates and minimal matrix interference. The compound was also utilized for the detection of UA in HeLa cells. Also, chemically modified paper strips were developed for rapid, on-site detection of UA.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0