ACS Applied Bio Materials最新文献

{"title":"Multimodal Synergistic Antimicrobial Activity of the Copper-Doped and Oxygen-Defective In Situ Nanocoating on Medical Titanium","authors":"Leizi Chi,&nbsp;Jinteng Qi,&nbsp;Zhuo Ma*,&nbsp;Zeshuai Zhang,&nbsp;Yunfeng Qiu*,&nbsp;Tiedong Sun* and Shaoqin Liu*,&nbsp;","doi":"10.1021/acsabm.5c0037610.1021/acsabm.5c00376","DOIUrl":"https://doi.org/10.1021/acsabm.5c00376https://doi.org/10.1021/acsabm.5c00376","url":null,"abstract":"<p >To combat escalating antibiotic resistance in titanium implant-associated infections, oxygen-vacancy-rich polydopamine/TiCu nanocoating (PDA/p-TiCu-300 °C) was developed on medical-grade titanium, uniquely enabling synergistic photothermal (PTT), photodynamic (PDT), and sonodynamic (SDT) antimicrobial strategies. Unlike previous dual-modal approaches, this trimodal strategy, activated by near-infrared light and ultrasound, achieved exceptional broad-spectrum bactericidal efficacy against both <i>Escherichia coli</i> (99.19% killing) and <i>Staphylococcus aureus</i> (95.03% killing) via enhanced reactive oxygen species (ROS) generation and membrane disruption. The engineered oxygen vacancies within the PDA/p-TiCu-300 °C nanocoating significantly boosted ROS production, outperforming conventional photocatalytic materials. Crucially, the nanocoatings demonstrated excellent <i>in vitro</i> cytocompatibility. This PTT–PDT–SDT platform exhibits synergistic multimodal bactericidal activity, overcoming the limitations of existing strategies and representing a paradigm shift in implant surface modification with significant translational potential against severe infections.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 4","pages":"3560–3570 3560–3570"},"PeriodicalIF":4.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Isolation and Characterization of Exosomes through a Single-Step, Label-Free Protein Biomarker Analysis","authors":"Hannah Ambrosius,&nbsp;Manjusha Vaidya,&nbsp;Fnu Joshua,&nbsp;Arjun Bajaj,&nbsp;Lei Zhai,&nbsp;Kiminobu Sugaya* and Qun Huo*,&nbsp;","doi":"10.1021/acsabm.5c0031810.1021/acsabm.5c00318","DOIUrl":"https://doi.org/10.1021/acsabm.5c00318https://doi.org/10.1021/acsabm.5c00318","url":null,"abstract":"<p >Exosomes, small extracellular vesicles with compositions reflecting their cell of origin, serve as sensitive and specific biomarkers for disease detection. We herein report a protocol for rapid isolation and characterization of exosomes by a single-step and label-free protein biomarker analysis. Using a simple centrifugation-filtration-concentration (CFC) method, exosomes are isolated and enriched 50-fold from conditioned cell culture media. For protein biomarker analysis, unconjugated antibodies are added directly to the isolated exosome solution. The specific interaction between the antibodies and exosomes leads to aggregation of exosomes, and subsequently, an average particle size increase of the assay solution. This average particle size increase can be detected using dynamic light scattering and correlated to the presence or absence of protein biomarkers on the exosomes. In this study, exosomes from three cell types, human embryonic kidney (HEK293) cells, genetically modified HEK-GFP cells, and GBM/NSC CD133⁺ cells were isolated. The exosomes released from HEK293, HEK-GFP, and GBM/NSC CD133⁺ cells exhibited monodispersed size distributions with an average particle size centered around 70, 66, and 249 nm, respectively. Positive antibody binding to exosome surface proteins resulted in a peak shift, increasing particle size by 25, 32, and 148 nm, respectively, for the HEK293, HEK-GFP, and GBM/NSC CD133⁺ exosomes, while the size increase upon addition of a negative antibody remained minimum. This protocol provides a convenient platform for the design and development of rapid diagnostic tests targeting disease specific exosomes.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 4","pages":"3533–3540 3533–3540"},"PeriodicalIF":4.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Nanoenabled Microalgae Systems: Integrating Oxidative Stress-Induced Metabolic Reprogramming and Enhanced Lipid Biosynthesis for Next-Generation Biofuel Production","authors":"Luis Pablo Salmeron Covarrubias,&nbsp;Kavitha Beluri,&nbsp;Yasaman Mohammadi,&nbsp;Nusrat Easmin,&nbsp;Oskar A. Palacios and Hamidreza Sharifan*,&nbsp;","doi":"10.1021/acsabm.5c0030010.1021/acsabm.5c00300","DOIUrl":"https://doi.org/10.1021/acsabm.5c00300https://doi.org/10.1021/acsabm.5c00300","url":null,"abstract":"<p >The growing demand for renewable energy has positioned microalgae, such as <i>Chlorella vulgaris</i>, as a promising feedstock for sustainable biofuel production. Leveraging nanotechnology, this study explores the multifaceted impacts of zinc oxide (ZnO) nanoparticles (NPs) on <i>C. vulgaris</i>, focusing on lipid biosynthesis, oxidative stress, biomass productivity, and photosynthetic pigment retention. The morphology of NPs and algae and their interactions were extensively studied using scanning electron microscopy (SEM), confocal microscopy, energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). The ZnO NP-enabled microalgae system enhanced lipid accumulation to as high as 48% at 50 mg/L. Biomass production and pigment content remained stable within the applied dose of NPs (20–50 mg/L), highlighting the resilience of <i>C. vulgaris</i> under NP exposure. However, at 100 mg/L, photosynthetic efficiency was disrupted, pigment content was reduced, and lipid yield declined to 30%. The enzymatic activity of catalase (CAT) revealed significant upregulation at higher ZnO NP concentrations, further corroborating the stress-induced metabolic shifts. This study also introduced a model for the Biofuel Suitability Score (BSS), which integrates lipid content, biomass productivity, oxidative stress levels, and pigment retention to identify the optimal conditions for biofuel production. The BSS peaked at moderate ZnO NP concentrations (30–50 mg/L), indicating a balance between lipid biosynthesis and cellular integrity. Beyond this threshold, oxidative damage compromises the biofuel potential, emphasizing the critical need for precise control of NP exposure. These findings highlight the potential of ZnO NPs to induce lipid accumulation through targeted stress modulation while maintaining biomass quality, advancing the application of nanotechnology in sustainable bioenergy systems. This study provides a scalable framework for integrating nanotechnology into renewable energy.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 4","pages":"3513–3524 3513–3524"},"PeriodicalIF":4.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsabm.5c00300","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Core–Shell UCNP@MOF Nanoplatforms for Dual Stimuli-Responsive Doxorubicin Release","authors":"Marina P. Abuçafy*,&nbsp;Beatriz B. S. Ramin,&nbsp;Angelica E. Graminha,&nbsp;Willy G. Santos,&nbsp;Regina C. G. Frem,&nbsp;Adelino V. G. Netto,&nbsp;José Clayston M. Pereira and Sidney J. L. Ribeiro*,&nbsp;","doi":"10.1021/acsabm.4c0179610.1021/acsabm.4c01796","DOIUrl":"https://doi.org/10.1021/acsabm.4c01796https://doi.org/10.1021/acsabm.4c01796","url":null,"abstract":"<p >Nanocarrier systems with multifunctional capabilities hold great potential for targeted cancer therapy, particularly for breast cancer treatment. Metal–organic frameworks (MOFs) are notable for their high porosity and, in some cases, biocompatibility, with ZIF-8 being particularly advantageous due to its pH-sensitive degradability, enabling selective drug release in tumor environments. Additionally, lanthanide-doped upconversion nanoparticles (UCNPs) offer unique optical properties that enhance both imaging and therapeutic applications. In this study, NaYF₄/Yb³⁺Er³⁺ UCNPs were synthesized via a hydrothermal method, subsequently coated with poly(acrylic acid) (PAA) and encapsulated within a ZIF-8 shell, forming of UCNP@ZIF-8 core–shell nanocomposites. This system was designed to leverage stimulation by a 980 nm laser and acidic pH to facilitate drug release. When exposed to this specific laser wavelength, the nanocomposites demonstrated significantly enhanced drug release, achieving up to 90% release of the incorporated antitumor drug, doxorubicin (DOX), in acidic environments. In vitro studies indicated selective cytotoxicity, with MCF-7 tumor cell viability decreasing from 85.7% to 20% following laser activation, while showing minimal toxicity toward healthy cells. These findings underscore the potential of the UCNP@ZIF-8 nanocarrier system as a pH and laser-responsive platform for improved cancer therapy, enabling precise control over drug delivery while minimizing side effects on surrounding healthy tissues.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 4","pages":"2954–2964 2954–2964"},"PeriodicalIF":4.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsabm.4c01796","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SnO2NPs as a Nontoxic Antiviral Agent for Designing Protective Masks against Human Coronavirus Infection","authors":"Anna Baranowska-Korczyc*,&nbsp;Dorota Kowalczyk,&nbsp;Marcin Chodkowski,&nbsp;Kamil Sobczak,&nbsp;Małgorzata Krzyżowska and Małgorzata Cieślak,&nbsp;","doi":"10.1021/acsabm.5c0017310.1021/acsabm.5c00173","DOIUrl":"https://doi.org/10.1021/acsabm.5c00173https://doi.org/10.1021/acsabm.5c00173","url":null,"abstract":"<p >The COVID-19 pandemic has created a need to develop protective textiles that reduce the infection of SARS-CoV-2, mainly via face masks. The key issue in designing protective textiles is the functionalization with antiviral agents. This report presents tin oxide nanoparticles (SnO₂NPs) as a novel, efficient antiviral agent against human coronavirus HCoV 229E due to blocking virus entry, attachment, and penetration into MRC-5 cells and nontoxicity. SnO₂NPs were obtained by sodium stannate hydrolysis and have a 3 nm diameter, a cubic structure, and a zeta potential of −28.8. SnO₂NPs were applied to functionalize a protective face mask made of silk fibroin. Polydopamine was applied to immobilize the particles. SnO₂NPs have a negative potential and enhance silk fabric hydrophobicity, which is crucial for antiviral properties. The mask functionalized with SnO₂NPs reveals very good antiviral properties and antibacterial activity against Gram-positive and -negative bacteria. Silk fabric functionalized with SnO₂NPs retains the silk fibroin β-sheet structure, is nontoxic, noncorrosive to human skin, and reveals high thermophysiological wear comfort.The highest filtration efficiency is obtained for the 3-layered mask (60%), while breathing resistance, sufficient for the FFP3 mask, was achieved for the 1-layered mask (maximum allowable breathing of 100 and 300 Pa, respectively, for 30 L/min and 95 L/min inhale and 300 Pa for an exhale flow rate of 160 L/min). SnO₂NPs can be useful in developing advanced antiviral textile materials to control virus spread and future pandemics.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 4","pages":"3416–3430 3416–3430"},"PeriodicalIF":4.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsabm.5c00173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Nanoenabled Microalgae Systems: Integrating Oxidative Stress-Induced Metabolic Reprogramming and Enhanced Lipid Biosynthesis for Next-Generation Biofuel Production.","authors":"Luis Pablo Salmeron Covarrubias, Kavitha Beluri, Yasaman Mohammadi, Nusrat Easmin, Oskar A Palacios, Hamidreza Sharifan","doi":"10.1021/acsabm.5c00300","DOIUrl":"https://doi.org/10.1021/acsabm.5c00300","url":null,"abstract":"<p><p>The growing demand for renewable energy has positioned microalgae, such as <i>Chlorella vulgaris</i>, as a promising feedstock for sustainable biofuel production. Leveraging nanotechnology, this study explores the multifaceted impacts of zinc oxide (ZnO) nanoparticles (NPs) on <i>C. vulgaris</i>, focusing on lipid biosynthesis, oxidative stress, biomass productivity, and photosynthetic pigment retention. The morphology of NPs and algae and their interactions were extensively studied using scanning electron microscopy (SEM), confocal microscopy, energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). The ZnO NP-enabled microalgae system enhanced lipid accumulation to as high as 48% at 50 mg/L. Biomass production and pigment content remained stable within the applied dose of NPs (20-50 mg/L), highlighting the resilience of <i>C. vulgaris</i> under NP exposure. However, at 100 mg/L, photosynthetic efficiency was disrupted, pigment content was reduced, and lipid yield declined to 30%. The enzymatic activity of catalase (CAT) revealed significant upregulation at higher ZnO NP concentrations, further corroborating the stress-induced metabolic shifts. This study also introduced a model for the Biofuel Suitability Score (BSS), which integrates lipid content, biomass productivity, oxidative stress levels, and pigment retention to identify the optimal conditions for biofuel production. The BSS peaked at moderate ZnO NP concentrations (30-50 mg/L), indicating a balance between lipid biosynthesis and cellular integrity. Beyond this threshold, oxidative damage compromises the biofuel potential, emphasizing the critical need for precise control of NP exposure. These findings highlight the potential of ZnO NPs to induce lipid accumulation through targeted stress modulation while maintaining biomass quality, advancing the application of nanotechnology in sustainable bioenergy systems. This study provides a scalable framework for integrating nanotechnology into renewable energy.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"YOPRO-1: A Cyanine-Based Molecular Rotor Probe for Amyloid Fibril Detection","authors":"Sailee D. Shahane,&nbsp;Niyati H. Mudliar,&nbsp;Bhavya R. Chawda,&nbsp;Munira Momin and Prabhat K. Singh*,&nbsp;","doi":"10.1021/acsabm.5c0018610.1021/acsabm.5c00186","DOIUrl":"https://doi.org/10.1021/acsabm.5c00186https://doi.org/10.1021/acsabm.5c00186","url":null,"abstract":"<p >The widespread occurrence of amyloidosis in many neurodegenerative diseases, including Alzheimer’s, highlights the urgent need for early detection methods. Traditional approaches often fall short in sensitivity, specificity, and the ability to operate within complex biological matrices. Fluorescence spectroscopy, which leverages the unique properties of extrinsic fluorescence sensors, has emerged as a promising avenue for amyloid detection. Thioflavin-T (ThT), while extensively utilized, faces several disadvantages such as poor blood–brain barrier penetration, short emission wavelength, and lack of sensitivity to oligomeric protein aggregates. These limitations necessitate the development of improved amyloid probes with enhanced properties for the better detection and understanding of neurodegenerative diseases. In this context, YOPRO-1, a cyanine-based molecular rotor probe, has been identified as a potent amyloid fibril sensor characterized by its turn-on fluorescence response and specificity for amyloid fibrils over native protein forms. Utilizing a variety of spectroscopic techniques, including steady-state emission, ground-state absorption, time-resolved fluorescence, and molecular docking, we demonstrate the superior selectivity and sensitivity of YOPRO-1 for amyloid fibrils. The probe exhibits a remarkable 245-fold increase in fluorescence intensity upon binding to insulin fibrils, which is a common amyloid model. This capability facilitates its application in complex biological matrices, such as high-percentage human serum, which has rarely been demonstrated by previous amyloid sensing probes. Furthermore, the commercial availability of YOPRO-1 avoids the challenges associated with the synthesis of specific probes, thereby marking a significant advancement in amyloid detection methodologies. Our findings highlight the potential of YOPRO-1 as a versatile and effective tool for the early diagnosis of amyloid-related diseases, offering a foundation for future therapeutic and diagnostic applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 4","pages":"3443–3453 3443–3453"},"PeriodicalIF":4.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redefining Medical Applications with Safe and Sustainable 3D Printing.","authors":"Sadaf Bashir Khan, Syed Irfan, Zhengjun Zhang, Weifeng Yuan","doi":"10.1021/acsabm.4c01923","DOIUrl":"https://doi.org/10.1021/acsabm.4c01923","url":null,"abstract":"<p><p>Additive manufacturing (AM) has revolutionized biomedical applications by enabling personalized designs, intricate geometries, and cost-effective solutions. This progress stems from interdisciplinary collaborations across medicine, biomaterials, engineering, artificial intelligence, and microelectronics. A pivotal aspect of AM is the development of materials that respond to stimuli such as heat, light, moisture, and chemical changes, paving the way for intelligent systems tailored to specific needs. Among the materials employed in AM, polymers have gained prominence due to their flexibility, synthetic versatility, and broad property spectrum. Their adaptability has made them the most widely used material class in AM processes, offering the potential for diverse applications, including surgical tools, structural composites, photovoltaic devices, and filtration systems. Despite this, integrating multiple polymer systems to achieve multifunctional and dynamic performance remains a significant challenge, highlighting the need for further research. This review explores the foundational principles of AM, emphasizing its application in tissue engineering and medical technologies. It provides an in-depth analysis of polymer systems, besides inorganic oxides and bioinks, and examines their unique properties, advantages, and limitations within the context of AM. Additionally, the review highlights emerging techniques like rapid prototyping and 3D printing, which hold promise for advancing biomedical applications. By addressing the critical factors influencing AM processes and proposing innovative approaches to polymer integration, this review aims to guide future research and development in the field. The insights presented here underscore the transformative potential of AM in creating dynamic, multifunctional systems to meet evolving biomedical and healthcare demands.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-Infrared Multifunctional Antitumor Nanomaterial of Bismuth Sulfide Heterojunction Coupled with Mesoporous Polydopamine","authors":"Juanxuan Guo,&nbsp;Biling Chen,&nbsp;Menglong Zhao,&nbsp;Wenbin Chen,&nbsp;Siqi Qiu*,&nbsp;Ming-De Li* and Li Dang*,&nbsp;","doi":"10.1021/acsabm.5c0031310.1021/acsabm.5c00313","DOIUrl":"https://doi.org/10.1021/acsabm.5c00313https://doi.org/10.1021/acsabm.5c00313","url":null,"abstract":"<p >Bismuth sulfide@bismuth nanorods (Bi₂S₃@Bi NRs) have emerged as promising photodynamic therapeutic agents due to Bi₂S₃@Bi being able to produce reactive oxygen species from self-supplied O₂. Combining photothermal and photodynamic therapies with chemotherapy is attractive but difficult to achieve. Here, we develop a subtle method to wrap Bi₂S₃@Bi NRs with photothermal mesoporous polydopamine, where chemotherapy drug doxorubicin hydrochloride can be loaded, thus providing multifunctional antitumor nanospheres. To our delight, the prepared triple-functional material exhibits excellent antitumor efficacy toward tumor cells under near-infrared light irradiation. This multifunctional antitumor nanomaterial is not only biocompatible but also suitable for tumor hypoxic microenvironments, having much better efficacy than single- or double-functional materials. This study highlights the great potential of combining photothermal, photodynamic, and chemotherapies.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 4","pages":"3525–3532 3525–3532"},"PeriodicalIF":4.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-Infrared Light-Controlled Nitric Oxide Delivery Combined with In Situ Activated Chemotherapy for Enhanced Multimodal Therapy.","authors":"Bing Ren, Jing Liu, Yi Wang, Qi Tang, Jian Fang, Shiping Yang, Jin-Gang Liu","doi":"10.1021/acsabm.5c00175","DOIUrl":"https://doi.org/10.1021/acsabm.5c00175","url":null,"abstract":"<p><p>Development of nanoplatforms with in situ activation for chemotherapy represents a promising modality for biomedical application. Herein, a multifunctional nanoplatform, CMS@DTC@PDA@RuNO@FA (abbreviated as CDPNF NPs), was developed for highly efficient antitumor therapy, in which diethyldithiocarbamate (DTC)-loaded mesoporous Cu₂MoS₄ (CMS) nanoparticles were covered by polydopamine (PDA) layers and further covalently modified with a NO donor (RuNO) and a folic acid (FA)-directing moiety. Under the mild acidic tumor microenvironment (TME), the CDPNF NPs co-liberated DTC and Cu²⁺ in the tumor site, where in situ formation of the highly cytotoxic Cu(DTC)₂ complex effectively killed tumor cells. Furthermore, under near-infrared (NIR) light irradiation, the CDPNF NPs could deliver nitric oxide (NO) and produce superoxide anions (O₂^•-), followed by the formation of more toxic peroxynitrite (ONOO^-), which led to promoted cell apoptosis. Under 1064 nm NIR light irradiation, in vivo experiments with CDPNF NPs demonstrated an impressively high tumor inhibition rate (∼97%) while with good biocompatibility. This work represents an in situ activated approach for precision medicine that might imply its promising potential for clinical applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}