ACS Applied Bio Materials最新文献

{"title":"Bifunctional Metal Ion-Enhanced PDA-Coated Titanium for Superior Osteogenic and Antimicrobial Performance.","authors":"Chenhui Liu, Sikun Meng, Huachun Wang, Tianzhen Zhang, Cheng Qi, Mei Li, Jingyang Kang","doi":"10.1021/acsabm.4c01869","DOIUrl":"https://doi.org/10.1021/acsabm.4c01869","url":null,"abstract":"<p><p>Titanium (Ti) implants serve as a popular solution for tooth replacement, because they combine excellent mechanical properties with high biocompatibility. However, the biologically inert nature of titanium surfaces restricts their osteogenic capacity and renders them prone to bacterial colonization, elevating the risk of biofilm formation and implant-associated infections. Surface modification of titanium implants is essential to overcoming these limitations. To optimize the titanium surface functionality, this study employed a polydopamine (PDA) coating strategy combined with subsequent incorporation of copper and manganese ions. We prepared Ti-PDA and then fabricated Ti-PDA-Cu and Ti-PDA-Cu/Mn samples. Material characterization revealed that Ti-PDA-Cu/Mn exhibits favorable surface wettability and biocompatibility, along with sustained and stable release of Cu²⁺ and Mn²⁺ ions. Experiments in vitro showed that Ti-PDA-Cu/Mn had good antimicrobial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>, significantly promoted protein adsorption, osteoblast adhesion, proliferation, and mineralization, and upregulated the expression of osteogenic genes. A promising strategy has been established through this bifunctional modification to simultaneously enhance the osteogenic and antimicrobial properties of titanium implants.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibroblast Morphology, Adhesion, and Extracellular Matrix Secretion in Columnar Microarray Hydroxyapatite.","authors":"Takuma Watanabe, Gerardo Martin Quindoza, Yuta Aida, Hayato Laurence Mizuno, Yasutaka Anraku, Toshiyuki Ikoma","doi":"10.1021/acsabm.5c00595","DOIUrl":"https://doi.org/10.1021/acsabm.5c00595","url":null,"abstract":"<p><p>Biomimetic dental implants mimicking the columnar structure and biomechanics of natural enamel have been extensively studied. However, fibrointegration, a process essential for implant stability, remains largely underexplored. This study investigated the adhesion of NIH3T3 fibroblasts and extracellular matrix (ECM) secretion on hydroxyapatite (HAp) ceramic bodies with a columnar microarray architecture. Scanning electron microscopy and immunofluorescence analyses revealed dendritic fibroblast morphology, with protrusions penetrating and anchoring to parallel-aligned HAp pillars. ECM infiltration and fibrous structures along the pillars were also observed. These findings suggest that the enamel-like structure enhances fibrointegration, potentially reducing the risk of implant failure.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Cellulose-Based Laser-Scribed Graphene Electrode for Hydrogen Peroxide Detection in Cancer Cells.","authors":"Lucas F de Lima, André L Ferreira, Letícia Ester Dos Santos, Keyla Lívian P Coelho, Keyla Teixeira Santos, Ariane Schmidt, Marcelo Bispo de Jesus, Thiago R L C Paixão, William R de Araujo","doi":"10.1021/acsabm.5c00825","DOIUrl":"https://doi.org/10.1021/acsabm.5c00825","url":null,"abstract":"<p><p>The development of sustainable and high-performance electrochemical sensors is crucial for advancing biomedical applications. In this work, we introduce a hydrogen peroxide (H₂O₂) sensor based on bacterial cellulose-derived laser-scribed graphene (BC-LSG), modified with MXene and platinum nanoparticles (PtNPs). Bacterial cellulose (BC), a biodegradable and renewable material, was cultivated and transformed into a highly conductive carbon network using CO₂ laser irradiation, producing a flexible, portable, and miniaturized electrochemical platform. The incorporation of MXene and PtNPs significantly enhanced the electrocatalytic response toward H₂O₂ oxidation, achieving a wide linear concentration range (15-95 μmol L^-1) and a low detection limit (0.35 μmol L^-1). Compared to traditional enzymatic sensors, our nanostructured BC-LSG device offers superior stability, reproducibility, and eco-friendliness, aligning with green analytical chemistry principles. The sensor was successfully applied for H₂O₂ detection in mammalian cells, demonstrating its potential for real-time monitoring of oxidative stress, a key biomarker in cancer progression and therapeutic responses. This work underscores the synergy between biopolymeric materials, nanotechnology, and laser processing, opening new avenues for scalable, disposable, and sustainable electrochemical devices.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobody Coupled with Au Nanoparticle Cluster to Detect Survivin Protein by Lateral Flow Immunoassay.","authors":"Yujiao Tang, Xiaomin Zhao, Zhenlin Nie, Bangshun He, Dawei Deng","doi":"10.1021/acsabm.5c00745","DOIUrl":"https://doi.org/10.1021/acsabm.5c00745","url":null,"abstract":"<p><p>The monoclonal antibody (mAb) used in a traditional Lateral Flow Immunoassay (LFIA) has many limitations, particularly related to its poor affinity, specificity, and stability. In this study, a nanobody (VHH) was developed with improved tolerability and higher specificity for the target. The strong binding affinity between VHH and survivin was emphasized by simulated docking, demonstrating the feasibility and effectiveness of replacing mAb in the detection of urinary survivin based on LFIA. In addition, the superior performance of VHH compared with mAb under extreme conditions was highlighted. To further enhance the detection performance, VHH was conjugated with an Au nanoparticle cluster (AuNP cluster). The pore size structure of the AuNP cluster led to an increased abundance of AuNPs, which amplified the signal while providing adequate adsorption sites for VHH coupling, thereby achieving a synergistic enhancement in the sensitivity and specificity of the AuNP cluster-VHH probe. Consequently, the detection limit for survivin was established at 3.90 ng/mL, facilitating noninvasive detection and monitoring in early-stage bladder cancer and during post-treatment surveillance. This insight underscores the key role of VHH in enhancing the performance of LFIA and the potential of developing a promising detection tool based on urinary biomarkers.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitin-Core Chitosan-Sheath Nanowhiskers as a Multifunctional Bionanofiber Modality for Hydrogel Microspheres in a Micromolding-Based Fabrication-Conjugation Approach.","authors":"Alec K Zackin, Subhash Kalidindi, Hyunmin Yi","doi":"10.1021/acsabm.5c00632","DOIUrl":"https://doi.org/10.1021/acsabm.5c00632","url":null,"abstract":"<p><p>Simple and tunable production of macroporous hydrogel microparticles for rapid protein quantification in a suspension array format remains a major challenge. We exploit biologically derived rigid nanofibers as a multifunctional modality in a robust micromolding method using a postfabrication bioconjugation approach to address this challenge. Specifically, chitin-core chitosan-sheath nanowhiskers (CSNW) with a tunable amine titer are prepared under mild deacetylation reaction conditions. Transmission electron microscopy, dynamic light scattering, and dynamic viscosity measurements show rigid bionanofibers with substantially lower viscosity compared to solubilized linear forms of chitosan and other biopolymers, suggesting improved handling and manufacturability. Fluorescent labeling studies on polyacrylamide-based microspheres fabricated via micromolding indicate stable and uniform incorporation of CSNW in hydrogel microspheres and the readily tunable chemical functionality of CSNW. Further, reliable fabrication using acrylate-modified CSNW as the primary cross-linker, along with selective and improved protein conjugation kinetics, attests to the macroporous network of the hydrogel microparticles and illustrates the multifunctionality of CSNW. We thus envision that our approach in harnessing potent bionanofibers and micromolding can be readily extended to produce a wide variety of multifaceted microscale materials with a multitude of desirable features with improved performances for applications such as rapid biosensing and biodiagnostics.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uptake Mechanism of Riboflavin-Functionalized Superparamagnetic Iron Oxide Nanoparticles in Triple-Negative Breast Cancer Cells.","authors":"Sopon Nuchpun, Wid Mekseriwattana, Anna Solé-Porta, Bodee Nutho, Onrapak Reamtong, Patompon Wongtrakoongate, Anna Roig, Kanlaya Prapainop Katewongsa","doi":"10.1021/acsabm.5c00649","DOIUrl":"https://doi.org/10.1021/acsabm.5c00649","url":null,"abstract":"<p><p>Superparamagnetic iron oxide nanoparticles (SPIONs), which are widely used as contrast agents in magnetic resonance imaging and as magnetic hyperthermia agents in cancer therapy, can be functionalized with biological molecules to enhance their specificity, stability, and cellular interaction. Riboflavin (Rf), a crucial biomolecule in cellular metabolism, is a potentially effective targeting moiety that can be selectively transported via riboflavin transporters (RFVTs), which are often overexpressed in cancer cells, including breast cancer cells. Here, we synthesize Rf-functionalized SPIONs (Rf-SPIONs) with high colloidal stability and stronger cellular interaction with breast cancer cells (MCF-7, and MDA-MB-231) than with normal breast cells (MCF-10A). Notably, the uptake is highest in triple-negative breast cancer cells (MDA-MB-231), a highly aggressive and treatment-resistant subtype. A mechanistic study revealed that RFVT is expressed in breast cancer cells and plays an important role in Rf-SPIONs uptake via the RFVT-mediated pathway. These findings identify riboflavin-functionalized nanoparticles as a promising platform for targeted delivery, diagnostic imaging, and cancer therapeutics. Rf-based nanomaterials could also pave the way for precision targeting of Rf-dependent metabolic pathways in cancer and other diseases.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of Green-Synthesized Dy³⁺-Doped Iron Oxide Nanoparticle-Entrapped Liposomes for Synergistic Modulation of MRI Contrast, Magnetic Hyperthermia, and Combined Anticancer Efficacy.","authors":"Poornima Govindharaj, Deepa Murugan, Somlee Gupta, Arunkumar Dhayalan, Barid Baran Lahiri, Arup Dasgupta, Sanjeevi Kannan","doi":"10.1021/acsabm.5c00503","DOIUrl":"https://doi.org/10.1021/acsabm.5c00503","url":null,"abstract":"<p><p>Cancer treatment demands the development of multifunctional diagnostic tools and therapeutic agents with low toxicity and a high therapeutic index. The current work aimed to design multifunctional quercetin (QTN)-encapsulated Dy³⁺-doped magneto-liposomes as theranostic agents. Fe₃O₄ and Dy³⁺-doped Fe₃O₄ nanoparticles were synthesized with the aid of <i>Punica granatum</i> <i>L</i> fruit peel extract. The thin film hydration technique employed to encapsulate Dy³⁺-doped iron oxide nanoparticles (IONPs) in liposomes resulted in a small multilamellar vesicle size of ∼50 nm. The antioxidant capacity of QTN-encapsulated liposomes displayed improved efficacy and better radical oxygen scavenging (ROS) ability. The saturation magnetization initially increased upon Dy³⁺ addition, followed by a significant reduction at higher Dy³⁺ concentrations due to the formation of a mixed phase. Magnetic hyperthermia studies on Dy³⁺-doped magneto-liposomes revealed a superior heating efficiency of ∼95.0 ± 5.9 W/g_Fe at a biomedically relevant field-frequency range. Dy³⁺-doped magneto-liposomes increased both T₁ and T₂ contrast, especially with Dy³⁺ playing an effective role to shorten T₂. The presence of Dy³⁺ in magneto-liposomes induced an enhanced X-ray attenuation of ∼22.7 HU. <i>In vitro</i> studies on MG-63 cell lines envisaged better efficacy against cancer cells (∼20%), while negligible cytotoxicity has been revealed from tests conducted on noncancerous HEK293 cells. The results clearly showed the multimodal theranostic applications of quercetin-loaded Dy^3+-doped magneto-liposomes.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tribo-Mechanical and Antibacterial Performance of 3D Printed hBN/PEGDA Nanocomposites for Load-Bearing Tissue Engineering Applications.","authors":"Raju Kumar, Jenish Patel, Avinash Parashar, Ankur Chaurasia","doi":"10.1021/acsabm.5c00950","DOIUrl":"https://doi.org/10.1021/acsabm.5c00950","url":null,"abstract":"<p><p>Photo-cross-linkable polyethylene glycol diacrylate (PEGDA (Mn ≈ 700)) is gaining importance as a potential biomaterial for tissue engineering due to its excellent biocompatibility and its ability to imitate the structural and functional characteristics of native human tissues. However, the limited mechanical and tribological properties of PEGDA constrain its application in load-bearing tissue engineering. To address the challenges associated with the limited mechanical and tribological properties of PEGDA, an attempt has been made to enhance its performance by incorporating an inorganic nanofiller hexagonal boron nitride (hBN). Here, stereolithography (SLA), an additive manufacturing technique, was used to synthesize the hBN-reinforced PEGDA nanocomposite, rendering superior mechanical, thermal, and tribological properties. PEGDA and phenylbis (2,4,6-trimethylbenzoyl) phosphine oxide (BAPOs) photoinitiator were mixed with hBN nanoplatelets (with varying concentrations of 0.25%, 0.50%, 0.75%, and 1.0% w/w) to prepare a composite resin. The SLA-printed PEGDA and hBN/PEGDA nanocomposites were characterized meticulously by using mechanical, physical, thermal, and tribological characterization techniques. As a result, hBN-incorporated PEGDA nanocomposite samples demonstrated significant improvement in the tensile, compressive, and flexural strength at 71.62%, 76.22%, and 31.89%, respectively, compared to that of pristine PEGDA. The fractography of the fractured surfaces revealed a pure brittle fracture in both pristine PEGDA and hBN/PEGDA nanocomposite samples. In addition to evaluating mechanical strength, the tribological performance of PEGDA and its hBN-reinforced nanocomposites was also assessed, revealing a substantial reduction in wear and frictional force upon nanofiller incorporation. The 3D printed samples were also characterized by differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), and wettability test. DSC thermograms and wettability measurements indicated that both the glass transition temperature and the hydrophilicity of the nanocomposites increased with higher hBN weight concentrations. Furthermore, the antibacterial property tests were conducted with two Gram-positive and Gram-negative bacteria, and it was found that the hBN-incorporated PEGDA composite resin inhibits antimicrobial properties.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction of \"Chitosan-Stabilized CuO Nanostructure-Functionalized UV-Crosslinked PVA/Chitosan Electrospun Membrane as Enhanced Wound Dressing\".","authors":"M Nuruzzaman Khan, M Tarik Arafat, Taslim Ur Rashid, Papia Haque, Mohammed Mizanur Rahman","doi":"10.1021/acsabm.5c01078","DOIUrl":"https://doi.org/10.1021/acsabm.5c01078","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered Endosymbionts that Modulate Primary Macrophage Function and Attenuate Tumor Growth by Shifting the Tumor Microenvironment.","authors":"Cody S Madsen, Ashley V Makela, Chima V Maduka, Emily M Greeson, Anthony Tundo, Evran Ural, Satyajit Hari Kulkarni, Ahmed A Zarea, Matti Kiupel, Maryam Sayadi, Christopher H Contag","doi":"10.1021/acsabm.5c00590","DOIUrl":"https://doi.org/10.1021/acsabm.5c00590","url":null,"abstract":"<p><p>Modulating gene expression in macrophages can be used to improve tissue regeneration and redirect tumor microenvironments (TMEs) toward positive therapeutic outcomes. We have developed <i>Bacillus subtilis</i> as an engineered endosymbiont (EES) capable of residing inside the eukaryotic host cell cytoplasm and controlling the fate of macrophages. Secretion of mammalian transcription factors (TFs) from <i>B. subtilis</i> that expresses listeriolysin O (LLO; allowing the EES to escape destruction by the macrophage) modulated expression of surface markers, cytokines, and chemokines, indicating functional changes in a macrophage/monocyte cell line. The engineered <i>B. subtilis</i> LLO TF strains were evaluated in murine bone marrow-derived macrophages (BMDMs) by flow cytometry, chemokine/cytokine profiling, metabolic assays, and RNA-Seq delivery of TFs by the EES shifted BMDM gene expression, production of cytokine and chemokines, and metabolic patterns, indicating that the TF strains could guide primary macrophage function. Thereafter, the ability of the TF strains to alter the TME was characterized in vivo in an orthotopic murine model of triple-negative breast cancer to assess therapeutic effects. The TF strains altered the TME by shifting immune cell composition and attenuating tumor growth. Additionally, multiple doses of the TF strains were well-tolerated by the mice. The use of <i>B. subtilis</i> LLO TF strains as EES showed promise as a unique cancer immunotherapy by directing the immune function intracellularly. The uses of EES could be expanded to modulate other mammalian cells over a range of biomedical applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}