Yujiao Tang, Xiaomin Zhao, Zhenlin Nie, Bangshun He, Dawei Deng
{"title":"Nanobody Coupled with Au Nanoparticle Cluster to Detect Survivin Protein by Lateral Flow Immunoassay.","authors":"Yujiao Tang, Xiaomin Zhao, Zhenlin Nie, Bangshun He, Dawei Deng","doi":"10.1021/acsabm.5c00745","DOIUrl":"https://doi.org/10.1021/acsabm.5c00745","url":null,"abstract":"<p><p>The monoclonal antibody (mAb) used in a traditional Lateral Flow Immunoassay (LFIA) has many limitations, particularly related to its poor affinity, specificity, and stability. In this study, a nanobody (VHH) was developed with improved tolerability and higher specificity for the target. The strong binding affinity between VHH and survivin was emphasized by simulated docking, demonstrating the feasibility and effectiveness of replacing mAb in the detection of urinary survivin based on LFIA. In addition, the superior performance of VHH compared with mAb under extreme conditions was highlighted. To further enhance the detection performance, VHH was conjugated with an Au nanoparticle cluster (AuNP cluster). The pore size structure of the AuNP cluster led to an increased abundance of AuNPs, which amplified the signal while providing adequate adsorption sites for VHH coupling, thereby achieving a synergistic enhancement in the sensitivity and specificity of the AuNP cluster-VHH probe. Consequently, the detection limit for survivin was established at 3.90 ng/mL, facilitating noninvasive detection and monitoring in early-stage bladder cancer and during post-treatment surveillance. This insight underscores the key role of VHH in enhancing the performance of LFIA and the potential of developing a promising detection tool based on urinary biomarkers.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chitin-Core Chitosan-Sheath Nanowhiskers as a Multifunctional Bionanofiber Modality for Hydrogel Microspheres in a Micromolding-Based Fabrication-Conjugation Approach.","authors":"Alec K Zackin, Subhash Kalidindi, Hyunmin Yi","doi":"10.1021/acsabm.5c00632","DOIUrl":"https://doi.org/10.1021/acsabm.5c00632","url":null,"abstract":"<p><p>Simple and tunable production of macroporous hydrogel microparticles for rapid protein quantification in a suspension array format remains a major challenge. We exploit biologically derived rigid nanofibers as a multifunctional modality in a robust micromolding method using a postfabrication bioconjugation approach to address this challenge. Specifically, chitin-core chitosan-sheath nanowhiskers (CSNW) with a tunable amine titer are prepared under mild deacetylation reaction conditions. Transmission electron microscopy, dynamic light scattering, and dynamic viscosity measurements show rigid bionanofibers with substantially lower viscosity compared to solubilized linear forms of chitosan and other biopolymers, suggesting improved handling and manufacturability. Fluorescent labeling studies on polyacrylamide-based microspheres fabricated via micromolding indicate stable and uniform incorporation of CSNW in hydrogel microspheres and the readily tunable chemical functionality of CSNW. Further, reliable fabrication using acrylate-modified CSNW as the primary cross-linker, along with selective and improved protein conjugation kinetics, attests to the macroporous network of the hydrogel microparticles and illustrates the multifunctionality of CSNW. We thus envision that our approach in harnessing potent bionanofibers and micromolding can be readily extended to produce a wide variety of multifaceted microscale materials with a multitude of desirable features with improved performances for applications such as rapid biosensing and biodiagnostics.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sopon Nuchpun, Wid Mekseriwattana, Anna Solé-Porta, Bodee Nutho, Onrapak Reamtong, Patompon Wongtrakoongate, Anna Roig, Kanlaya Prapainop Katewongsa
{"title":"Uptake Mechanism of Riboflavin-Functionalized Superparamagnetic Iron Oxide Nanoparticles in Triple-Negative Breast Cancer Cells.","authors":"Sopon Nuchpun, Wid Mekseriwattana, Anna Solé-Porta, Bodee Nutho, Onrapak Reamtong, Patompon Wongtrakoongate, Anna Roig, Kanlaya Prapainop Katewongsa","doi":"10.1021/acsabm.5c00649","DOIUrl":"https://doi.org/10.1021/acsabm.5c00649","url":null,"abstract":"<p><p>Superparamagnetic iron oxide nanoparticles (SPIONs), which are widely used as contrast agents in magnetic resonance imaging and as magnetic hyperthermia agents in cancer therapy, can be functionalized with biological molecules to enhance their specificity, stability, and cellular interaction. Riboflavin (Rf), a crucial biomolecule in cellular metabolism, is a potentially effective targeting moiety that can be selectively transported via riboflavin transporters (RFVTs), which are often overexpressed in cancer cells, including breast cancer cells. Here, we synthesize Rf-functionalized SPIONs (Rf-SPIONs) with high colloidal stability and stronger cellular interaction with breast cancer cells (MCF-7, and MDA-MB-231) than with normal breast cells (MCF-10A). Notably, the uptake is highest in triple-negative breast cancer cells (MDA-MB-231), a highly aggressive and treatment-resistant subtype. A mechanistic study revealed that RFVT is expressed in breast cancer cells and plays an important role in Rf-SPIONs uptake via the RFVT-mediated pathway. These findings identify riboflavin-functionalized nanoparticles as a promising platform for targeted delivery, diagnostic imaging, and cancer therapeutics. Rf-based nanomaterials could also pave the way for precision targeting of Rf-dependent metabolic pathways in cancer and other diseases.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Design of Green-Synthesized Dy<sup>3+</sup>-Doped Iron Oxide Nanoparticle-Entrapped Liposomes for Synergistic Modulation of MRI Contrast, Magnetic Hyperthermia, and Combined Anticancer Efficacy.","authors":"Poornima Govindharaj, Deepa Murugan, Somlee Gupta, Arunkumar Dhayalan, Barid Baran Lahiri, Arup Dasgupta, Sanjeevi Kannan","doi":"10.1021/acsabm.5c00503","DOIUrl":"https://doi.org/10.1021/acsabm.5c00503","url":null,"abstract":"<p><p>Cancer treatment demands the development of multifunctional diagnostic tools and therapeutic agents with low toxicity and a high therapeutic index. The current work aimed to design multifunctional quercetin (QTN)-encapsulated Dy<sup>3+</sup>-doped magneto-liposomes as theranostic agents. Fe<sub>3</sub>O<sub>4</sub> and Dy<sup>3+</sup>-doped Fe<sub>3</sub>O<sub>4</sub> nanoparticles were synthesized with the aid of <i>Punica granatum</i> <i>L</i> fruit peel extract. The thin film hydration technique employed to encapsulate Dy<sup>3+</sup>-doped iron oxide nanoparticles (IONPs) in liposomes resulted in a small multilamellar vesicle size of ∼50 nm. The antioxidant capacity of QTN-encapsulated liposomes displayed improved efficacy and better radical oxygen scavenging (ROS) ability. The saturation magnetization initially increased upon Dy<sup>3+</sup> addition, followed by a significant reduction at higher Dy<sup>3+</sup> concentrations due to the formation of a mixed phase. Magnetic hyperthermia studies on Dy<sup>3+</sup>-doped magneto-liposomes revealed a superior heating efficiency of ∼95.0 ± 5.9 W/g<sub>Fe</sub> at a biomedically relevant field-frequency range. Dy<sup>3+</sup>-doped magneto-liposomes increased both T<sub>1</sub> and T<sub>2</sub> contrast, especially with Dy<sup>3+</sup> playing an effective role to shorten T<sub>2</sub>. The presence of Dy<sup>3+</sup> in magneto-liposomes induced an enhanced X-ray attenuation of ∼22.7 HU. <i>In vitro</i> studies on MG-63 cell lines envisaged better efficacy against cancer cells (∼20%), while negligible cytotoxicity has been revealed from tests conducted on noncancerous HEK293 cells. The results clearly showed the multimodal theranostic applications of quercetin-loaded Dy<sup>3+-</sup>doped magneto-liposomes.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tribo-Mechanical and Antibacterial Performance of 3D Printed hBN/PEGDA Nanocomposites for Load-Bearing Tissue Engineering Applications.","authors":"Raju Kumar, Jenish Patel, Avinash Parashar, Ankur Chaurasia","doi":"10.1021/acsabm.5c00950","DOIUrl":"https://doi.org/10.1021/acsabm.5c00950","url":null,"abstract":"<p><p>Photo-cross-linkable polyethylene glycol diacrylate (PEGDA (Mn ≈ 700)) is gaining importance as a potential biomaterial for tissue engineering due to its excellent biocompatibility and its ability to imitate the structural and functional characteristics of native human tissues. However, the limited mechanical and tribological properties of PEGDA constrain its application in load-bearing tissue engineering. To address the challenges associated with the limited mechanical and tribological properties of PEGDA, an attempt has been made to enhance its performance by incorporating an inorganic nanofiller hexagonal boron nitride (hBN). Here, stereolithography (SLA), an additive manufacturing technique, was used to synthesize the hBN-reinforced PEGDA nanocomposite, rendering superior mechanical, thermal, and tribological properties. PEGDA and phenylbis (2,4,6-trimethylbenzoyl) phosphine oxide (BAPOs) photoinitiator were mixed with hBN nanoplatelets (with varying concentrations of 0.25%, 0.50%, 0.75%, and 1.0% w/w) to prepare a composite resin. The SLA-printed PEGDA and hBN/PEGDA nanocomposites were characterized meticulously by using mechanical, physical, thermal, and tribological characterization techniques. As a result, hBN-incorporated PEGDA nanocomposite samples demonstrated significant improvement in the tensile, compressive, and flexural strength at 71.62%, 76.22%, and 31.89%, respectively, compared to that of pristine PEGDA. The fractography of the fractured surfaces revealed a pure brittle fracture in both pristine PEGDA and hBN/PEGDA nanocomposite samples. In addition to evaluating mechanical strength, the tribological performance of PEGDA and its hBN-reinforced nanocomposites was also assessed, revealing a substantial reduction in wear and frictional force upon nanofiller incorporation. The 3D printed samples were also characterized by differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), and wettability test. DSC thermograms and wettability measurements indicated that both the glass transition temperature and the hydrophilicity of the nanocomposites increased with higher hBN weight concentrations. Furthermore, the antibacterial property tests were conducted with two Gram-positive and Gram-negative bacteria, and it was found that the hBN-incorporated PEGDA composite resin inhibits antimicrobial properties.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Nuruzzaman Khan, M Tarik Arafat, Taslim Ur Rashid, Papia Haque, Mohammed Mizanur Rahman
{"title":"Retraction of \"Chitosan-Stabilized CuO Nanostructure-Functionalized UV-Crosslinked PVA/Chitosan Electrospun Membrane as Enhanced Wound Dressing\".","authors":"M Nuruzzaman Khan, M Tarik Arafat, Taslim Ur Rashid, Papia Haque, Mohammed Mizanur Rahman","doi":"10.1021/acsabm.5c01078","DOIUrl":"https://doi.org/10.1021/acsabm.5c01078","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cody S Madsen, Ashley V Makela, Chima V Maduka, Emily M Greeson, Anthony Tundo, Evran Ural, Satyajit Hari Kulkarni, Ahmed A Zarea, Matti Kiupel, Maryam Sayadi, Christopher H Contag
{"title":"Engineered Endosymbionts that Modulate Primary Macrophage Function and Attenuate Tumor Growth by Shifting the Tumor Microenvironment.","authors":"Cody S Madsen, Ashley V Makela, Chima V Maduka, Emily M Greeson, Anthony Tundo, Evran Ural, Satyajit Hari Kulkarni, Ahmed A Zarea, Matti Kiupel, Maryam Sayadi, Christopher H Contag","doi":"10.1021/acsabm.5c00590","DOIUrl":"https://doi.org/10.1021/acsabm.5c00590","url":null,"abstract":"<p><p>Modulating gene expression in macrophages can be used to improve tissue regeneration and redirect tumor microenvironments (TMEs) toward positive therapeutic outcomes. We have developed <i>Bacillus subtilis</i> as an engineered endosymbiont (EES) capable of residing inside the eukaryotic host cell cytoplasm and controlling the fate of macrophages. Secretion of mammalian transcription factors (TFs) from <i>B. subtilis</i> that expresses listeriolysin O (LLO; allowing the EES to escape destruction by the macrophage) modulated expression of surface markers, cytokines, and chemokines, indicating functional changes in a macrophage/monocyte cell line. The engineered <i>B. subtilis</i> LLO TF strains were evaluated in murine bone marrow-derived macrophages (BMDMs) by flow cytometry, chemokine/cytokine profiling, metabolic assays, and RNA-Seq delivery of TFs by the EES shifted BMDM gene expression, production of cytokine and chemokines, and metabolic patterns, indicating that the TF strains could guide primary macrophage function. Thereafter, the ability of the TF strains to alter the TME was characterized in vivo in an orthotopic murine model of triple-negative breast cancer to assess therapeutic effects. The TF strains altered the TME by shifting immune cell composition and attenuating tumor growth. Additionally, multiple doses of the TF strains were well-tolerated by the mice. The use of <i>B. subtilis</i> LLO TF strains as EES showed promise as a unique cancer immunotherapy by directing the immune function intracellularly. The uses of EES could be expanded to modulate other mammalian cells over a range of biomedical applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yang Wang, Dianpeng Li, Min He, Gang Wang, Shulin Zhao
{"title":"Remodeling the Tumor Microenvironment via Metal-Phenolic Network-Coated Poly(lactic acid-<i>co</i>-glycolic acid) Nanoparticles for Inducing Multimodal Combination Therapy in Non-Small Cell Lung Cancer.","authors":"Yang Wang, Dianpeng Li, Min He, Gang Wang, Shulin Zhao","doi":"10.1021/acsabm.5c00634","DOIUrl":"https://doi.org/10.1021/acsabm.5c00634","url":null,"abstract":"<p><p>The treatment of non-small cell lung cancer (NSCLC) primarily relies on chemotherapy following surgical intervention. However, many late-stage NSCLC patients exhibit drug resistance and experience significant side effects from current chemotherapy agents, ultimately leading to disease progression. Consequently, the development of strategies to enhance efficacy while minimizing side effects through multimodal synergistic treatment technologies remains challenging. Here, we prepared a stable multifunctional nanocomplex, PLGA@DTX@Fe<sup>3+</sup>-TA/GO<sub>X</sub> (PDFTG), which utilizes poly(lactic acid-<i>co</i>-glycolic acid) (PLGA) nanoparticles to encapsulate docetaxel (DTX) as the core component, while a Fe<sup>3+</sup>-tannic acid (TA) nanonetwork serves as the outer coating. Additionally, interactions between polyphenols and proteins facilitate the loading of glucose oxidase (GO<sub>X</sub>). After intravenous injection, PDFTG selectively accumulates in the tumor region, subsequently releasing Fe<sup>3+</sup>, TA, DTX, and GO<sub>X</sub>, thereby remodeling the tumor microenvironment. Following this process, Fe<sup>3+</sup> is reduced to Fe<sup>2+</sup> by TA, leading to mitochondrial damage in cancer cells and resulting in an increase in O<sub>2</sub> content. This increase promotes the GO<sub>X</sub>-catalyzed glucose oxidation reaction that generates gluconic acid and H<sub>2</sub>O<sub>2</sub>, initiating starvation therapy. The gluconic acid enhances the acidity of the microenvironment, further promoting the release of PDFTG nanoparticles and enhancing the accumulation of H<sub>2</sub>O<sub>2</sub> within the cell. The accumulated H<sub>2</sub>O<sub>2</sub> reacts with Fe<sup>2+</sup> in a strongly acidic environment, leading to the of hydroxyl free radicals that initiate chemical dynamic therapy (CDT). Moreover, mitochondrial damage results in a reduction of adenosine triphosphate levels, which decreases the expression of P-glycoprotein, thereby reversing DTX resistance and producing potent multimodal synergistic antitumor effects. Multimode treatment strategies for reshaping the tumor microenvironment offer an approach with significant clinical application potential for the efficient treatment of NSCLC.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>In Silico</i> Design of Antimicrobial Peptides against Carbapenem-Resistant <i>Acinetobacter baumannii</i> Infections with Enhanced Activity by Nanoformulation.","authors":"Lawrance Richardson, Tsung-Ying Yang, Yu-Wei Chen, Shang-Yi Lin, Yeng-Tseng Wang, Po-Liang Lu, Yang-Hsiang Chan, Hong-Cheu Lin","doi":"10.1021/acsabm.5c00679","DOIUrl":"https://doi.org/10.1021/acsabm.5c00679","url":null,"abstract":"<p><p>Carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) has emerged as a critical public health menace. Its resistance to last-resort antibiotics highlights the urgent need for innovative treatment approaches. Antimicrobial peptides (AMPs) are promising candidates to address this challenge. AMPs have distinct mechanisms and a low likelihood of inducing resistance. In this study, we designed a water-soluble cationic AMP, \"T2-02.\" This was achieved using AMP database screening and <i>in silico</i> modeling with genetic algorithms (GAs). T2-02 has a net +7 charge at physiological pH and is composed of 21 amino acid residues. This charge facilitates strong electrostatic interactions with negatively charged microbial membranes. Moreover, the helical secondary structure of T2-02 enhances amphipathicity, enabling effective membrane insertion. When tested against Gram-negative CRAB isolates, T2-02 showed strong antibacterial activity. It also demonstrated outstanding biocompatibility, with low cytotoxicity and a minimal inhibitory concentration (MIC) of 8-16 μg/mL. Its therapeutic potential was further enhanced by the use of a liposomal nanodelivery method. This significantly improved T2-02's loading efficiency. The liposomal strategy amplified its antimicrobial efficacy, reducing MICs by 2- to 4-fold. It also further minimized cytotoxicity. These results position T2-02 as a promising candidate for combating CRAB infections.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"H<sub>2</sub>O<sub>2</sub>-Responsive Boronic Ester-Modified Mesoporous Silica Nanocarrier for TfR Mediated Tumor-Specific Drug Delivery Applications.","authors":"Hsiao-Yen Lee, Natesan Thirumalaivasan, Shu-Pao Wu","doi":"10.1021/acsabm.5c00645","DOIUrl":"https://doi.org/10.1021/acsabm.5c00645","url":null,"abstract":"<p><p>This study introduces a drug delivery system utilizing boronic ester-functionalized mesoporous silica nanoparticles (<b>MSNP-BA-Tf</b>) for targeted cancer therapy. By conjugating transferrin (Tf) to the MSNP surface, the system actively targets cancer cells via transferrin receptor (TfR)-mediated endocytosis. The incorporation of boronic ester linkages enables hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-responsive drug release, enhancing therapeutic efficacy. In vitro experiments demonstrated that <b>MSNP-BA-Tf</b> effectively delivered doxorubicin (DOX) to cancer cells while sparing normal cells, as confirmed by fluorescence imaging and cytotoxicity assays. In vivo studies using a colon cancer xenograft model showed that <b>DOX@MSNP-BA-Tf</b> inhibited tumor growth more effectively than free DOX. These findings highlight <b>MSNP-BA-Tf</b> as a promising nanocarrier for cancer therapy, offering targeted and controlled drug delivery through active targeting and H<sub>2</sub>O<sub>2</sub> responsiveness.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}