Jakub A Kochanowski, Bobby Carroll, Merrill E Asp, Emma C Kaputa, Alison E Patteson
{"title":"Bacteria Colonies Modify Their Shear and Compressive Mechanical Properties in Response to Different Growth Substrates.","authors":"Jakub A Kochanowski, Bobby Carroll, Merrill E Asp, Emma C Kaputa, Alison E Patteson","doi":"10.1021/acsabm.3c00907","DOIUrl":"10.1021/acsabm.3c00907","url":null,"abstract":"<p><p>Bacteria build multicellular communities termed biofilms, which are often encased in a self-secreted extracellular matrix that gives the community mechanical strength and protection against harsh chemicals. How bacteria assemble distinct multicellular structures in response to different environmental conditions remains incompletely understood. Here, we investigated the connection between bacteria colony mechanics and the colony growth substrate by measuring the oscillatory shear and compressive rheology of bacteria colonies grown on agar substrates. We found that bacteria colonies modify their own mechanical properties in response to shear and uniaxial compression in a manner that depends on the concentration of agar in their growth substrate. These findings highlight that mechanical interactions between bacteria and their microenvironments are an important element in bacteria colony development, which can aid in developing strategies to disrupt or reduce biofilm growth.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"7809-7817"},"PeriodicalIF":4.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139400907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacterial Patterning: A Promising Biofabrication Technique.","authors":"Minghui Xiao, Shuyi Lv, Chunlei Zhu","doi":"10.1021/acsabm.4c00056","DOIUrl":"10.1021/acsabm.4c00056","url":null,"abstract":"<p><p>Bacterial patterning has emerged as a pivotal biofabrication technique in the biomedical field. In the past 2 decades, a diverse array of bacterial patterning approaches have been developed to enable the precise manipulation of the spatial distribution of bacterial patterns for various applications. Despite the significance of these advancements, there is a deficiency of review articles providing an overview of bacterial patterning technologies. In this mini-review, we systematically summarize the progress of bacterial patterning over the past 2 decades. This review commences with an elucidation of the definition and fundamental principles of bacterial patterning. Subsequently, we introduce the established bacterial patterning strategies, accompanied by discussions about the advantages and limitations of each approach. Furthermore, we showcase the biomedical applications of these strategies, highlighting their efficacy in spatial control of biofilms, biosensing, and biointervention. Finally, this mini-review is concluded with a summary and an outlook on future challenges and opportunities. It is anticipated that this mini-review can serve as a concise guide for those who are interested in this exciting and rapidly evolving research area.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"8008-8018"},"PeriodicalIF":4.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139970163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nghia Le Ba Thai, Henry T Beaman, Megan Perlman, Ernest E Obeng, Changling Du, Mary Beth B Monroe
{"title":"Chitosan Poly(vinyl alcohol) Methacrylate Hydrogels for Tissue Engineering Scaffolds.","authors":"Nghia Le Ba Thai, Henry T Beaman, Megan Perlman, Ernest E Obeng, Changling Du, Mary Beth B Monroe","doi":"10.1021/acsabm.3c01209","DOIUrl":"10.1021/acsabm.3c01209","url":null,"abstract":"<p><p>A major challenge in tissue engineering scaffolds is controlling scaffold degradation rates during healing while maintaining mechanical properties to support tissue formation. Hydrogels are three-dimensional matrices that are widely applied as tissue scaffolds based on their unique properties that can mimic the extracellular matrix. In this study, we develop a hybrid natural/synthetic hydrogel platform to tune the properties for tissue engineering scaffold applications. We modified chitosan and poly(vinyl alcohol) (PVA) with photo-cross-linkable methacrylate functional groups and then synthesized a library of chitosan PVA methacrylate hydrogels (ChiPVAMA) with two different photoinitiators, Irgacure 2959 (I2959) and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP). ChiPVAMA hydrogels showed tunability in degradation rates and mechanical properties based on both the polymer content and photoinitiator type. This tunability could enable their application in a range of tissue scaffold applications. In a 2D scratch wound healing assay, all hydrogel samples induced faster wound closure compared to a gauze clinical wound dressing control. NIH/3T3 cells encapsulated in hydrogels showed a high viability (∼92%) over 14 days, demonstrating the capacity of this system as a supportive cell scaffold. In addition, hydrogels containing a higher chitosan content demonstrated a high antibacterial capacity. Overall, ChiPVAMA hydrogels provide a potential tissue engineering scaffold that is tunable, degradable, and suitable for cell growth.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"7818-7827"},"PeriodicalIF":4.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139911438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bianca B M Garcia, Stefania Douka, Omar Mertins, Enrico Mastrobattista, Sang W Han
{"title":"Efficacy of Chitosan-N-Arginine Chitosomes in mRNA Delivery and Cell Viability Enhancement.","authors":"Bianca B M Garcia, Stefania Douka, Omar Mertins, Enrico Mastrobattista, Sang W Han","doi":"10.1021/acsabm.4c00983","DOIUrl":"10.1021/acsabm.4c00983","url":null,"abstract":"<p><p>Cationic lipid-based carriers are recognized for their ability to complex with mRNA and effectively deliver the mRNA for vaccination and therapeutic purposes. However, the significant cytotoxicity of these carriers often restricts their practical application. In the present study, polymer-lipid hybrid nanoparticles, termed chitosomes, incorporating chitosan-N-arginine (CSA) with the DOTAP cationic lipid and the DOPE helper lipid, were synthesized and evaluated. The addition of CSA to the lipid formulations improved their physicochemical stability and enhanced mRNA complexation, resulting in high transfection rates in the HeLa and HEK293T cell lines. However, the transfection efficiency was low in the NIH-3T3 cell line, indicating a cell type-specific response to chitosomes. Importantly, CSA significantly reduced the cytotoxicity typically associated with DOTAP. Overall, the present study indicated that optimizing the ratio of CSA to DOTAP is crucial for developing mRNA nanocarriers to achieve high transfection efficiency and reduce cytotoxicity across different cell lines.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"8261-8271"},"PeriodicalIF":4.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ashwini Dantanarayana, Wassim El Housseini, Kevin Beaver, Monica Brachi, Timothy P McFadden, Shelley D Minteer
{"title":"Boosting the Microbial Electrosynthesis of Formate by <i>Shewanella oneidensis</i> MR-1 with an Ionic Liquid Cosolvent.","authors":"Ashwini Dantanarayana, Wassim El Housseini, Kevin Beaver, Monica Brachi, Timothy P McFadden, Shelley D Minteer","doi":"10.1021/acsabm.4c01276","DOIUrl":"10.1021/acsabm.4c01276","url":null,"abstract":"<p><p>Microbial electrosynthesis (MES) is a rapidly growing technology at the forefront of sustainable chemistry, leveraging the ability of microorganisms to catalyze electrochemical reactions to synthesize valuable compounds from renewable energy sources. The reduction of CO<sub>2</sub> is a major target application for MES, but research in this area has been stifled, especially with the use of direct electron transfer (DET)-based microbial systems. The major fundamental hurdle that needs to be overcome is the low efficiency of CO<sub>2</sub> reduction largely attributed to minimal microbial access to CO<sub>2</sub> owing to its low solubility in the electrolyte. With their tunable physical properties, ionic liquids present a potential solution to this challenge and have previously shown promise in facilitating efficient CO<sub>2</sub> electroreduction by increasing the CO<sub>2</sub> solubility. However, the use of ionic liquids in MES remains unexplored. In this study, we investigated the role of 1-ethyl-3-methylimidazolium acetate ([EMIM][Ac]) using <i>Shewanella oneidensis</i> MR-1 as a model DET strain. Electrochemical investigations demonstrated the ability of <i>S. oneidensis</i> MR-1 biocathodes to directly convert CO<sub>2</sub> to formate with a faradaic efficiency of 34.5 ± 26.1%. The addition of [EMIM][Ac] to the system significantly increased cathodic current density and enhanced the faradaic efficiency to 94.5 ± 4.3% while concurrently amplifying the product yield from 34 ± 23 μM to 366 ± 34 μM. These findings demonstrate that ionic liquids can serve as efficient, biocompatible cosolvents for microbial electrochemical reduction of CO<sub>2</sub> to value-added products, holding promise for more robust applications of MES.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"8434-8443"},"PeriodicalIF":4.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Improved Graphitization of Lignin by Templating Using Graphene Oxide Additives.","authors":"Sandra N Ike, Randy L Vander Wal","doi":"10.1021/acsabm.4c01122","DOIUrl":"10.1021/acsabm.4c01122","url":null,"abstract":"<p><p>Techniques to improve the graphitization of lignin, the second most abundant natural polymer, are in great demand as a viable means to obtain cost-effective and less energy-intensive graphite for various applications. In this work, we report the effects of two-dimensional nanomaterials, graphene oxide (GO) and its derivative, reduced graphene oxide (RGO), used as templating agents for the graphitization of alkali-derived lignin. The hypothesis is that during heat temperature treatment, the GO additives act as a template that allows the lignin matrix to align on its basal planes through π-π interactions. In addition, possible chemical bonding between the GO additives and lignin may extend the two planar frameworks. Results from X-ray diffraction and Raman spectroscopy showed improved graphitic quality in the lignin-GO and lignin-RGO samples compared to pure lignin at 2500 °C. Transmission electron microscopy images and selected area electron diffraction patterns also revealed ordered nanostructures and defined polycrystalline patterns in the lignin-GO and lignin-RGO samples. This work presents a method to synthesize graphitic-like materials using carbon-based templates with the advantage that there is no need for further purification of the final material as in the case of transition metal catalysts.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"8319-8327"},"PeriodicalIF":4.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chanwoo Kim, Hayeon Kim, Jinwoong Jo, Soyeon Kim, Arrhon Mae Bongo, Ho-Joong Kim, Jaesung Yang
{"title":"Moderately Heavy Atom-Substituted BODIPY Photosensitizer with Mitochondrial Targeting Ability for Imaging-Guided Photodynamic Therapy.","authors":"Chanwoo Kim, Hayeon Kim, Jinwoong Jo, Soyeon Kim, Arrhon Mae Bongo, Ho-Joong Kim, Jaesung Yang","doi":"10.1021/acsabm.4c01108","DOIUrl":"10.1021/acsabm.4c01108","url":null,"abstract":"<p><p>Advanced photodynamic therapy requires photosensitizers with targeting, diagnostic, and therapeutic properties. To fulfill this multifunctionality, we report the synthesis of two triphenylphosphonium (TPP)-functionalized boron-dipyrromethene (BODIPY) dyes, TPPB-H and TPPB-Br, which incorporate a hydrogen atom and dibrominated vinyl moiety at the 6-position of the BODIPY core, respectively. The heavy-atom effect of the moderately heavy bromine atoms allowed TPPB-Br to achieve a proper balance between the toxic singlet oxygen (<sup>1</sup>O<sub>2</sub>) production and fluorescence efficiencies. In this dye, the bromine atom-induced stimulation of the singlet-to-triplet intersystem crossing dynamics resulted in an approximately 45-fold increase in the <sup>1</sup>O<sub>2</sub> quantum yield with respect to that of the nonbrominated counterpart (0.0059 and 0.28 for TPPB-H and TPPB-Br, respectively). This increase was accompanied only a 2-fold reduction in the fluorescence quantum yield (0.54 and 0.22 for TPPB-H and TPPB-Br, respectively). During multicolor confocal laser scanning microscopy observations conducted using two carcinomas, MCF-7 and HeLa, both BODIPY dyes exhibited high targeting specificity toward cancer cell mitochondria owing to the TPP cation functionalization. The two dyes also showed the feasibility of fluorescence cell imaging; however, only the dibrominated BODIPY TPPB-Br manifested pronounced photocytotoxicity with half-maximal inhibitory concentrations of 0.12 and 0.77 μM obtained for MCF-7 and HeLa cells, respectively. These findings demonstrate the potential applicability of TPPB-Br as an imaging-guided photodynamic therapy agent with mitochondrial specificity.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"8294-8304"},"PeriodicalIF":4.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Lucía Campaña Perilla, Jaime Gomez-Bolivar, Mohamed L Merroun, Nadeem Joudeh, Athanasios Saragliadis, Anja Røyne, Dirk Linke, Pavlo Mikheenko
{"title":"Characterization of Bimetallic Pd-Fe Nanoparticles Synthesized in <i>Escherichia coli</i>.","authors":"Ana Lucía Campaña Perilla, Jaime Gomez-Bolivar, Mohamed L Merroun, Nadeem Joudeh, Athanasios Saragliadis, Anja Røyne, Dirk Linke, Pavlo Mikheenko","doi":"10.1021/acsabm.4c01354","DOIUrl":"10.1021/acsabm.4c01354","url":null,"abstract":"<p><p>Biologically mediated nanoparticle (NP) synthesis offers a reliable and sustainable alternative route for metal NP production. Compared with conventional chemical and physical production methods that require hazardous materials and considerable energy expenditure, some microorganisms can reduce metal ions into NPs during standard metabolic processes. However, to be considered a feasible commercial option, the properties and inherent activity of bio-NPs still need to be significantly improved. In this work, we present an <i>Escherichia coli</i>-mediated synthesis method for catalytically active Pd-Fe NPs. The produced biogenic Pd-Fe NPs with varying Fe content were characterized using complementary analytical techniques to assess their size, composition, and structural properties. In addition, their catalytic performance was assessed by using standardized chemical reactions. We demonstrate that the combination of Pd with Fe leads to synergistic effects that enhance the catalytic performance of Pd NPs and make biogenic Pd-Fe NPs excellent potential substitutes for currently used catalysts. Briefly, the apparent rates for the model reaction of 4-nitrophenol reduction to 4-aminophenol catalyzed by Pd-based nanoparticles were as high as 0.1312 min<sup>-1</sup> using bimetallic Pd-Fe NPs, which is far superior to the rates of monometallic Pd NPs counterparts. This study provides a feasible strategy for the synthesis of multimetallic Pd-based NPs using common microbial processes. It emphasizes the potential of biogenic Pd-Fe NPs as efficient and sustainable catalysts for hydrogenation reactions, offering an environmentally friendly synthesis for various applications, including wastewater treatment and the production of fine chemicals.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"8573-8589"},"PeriodicalIF":4.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ziyi Zhao, Boyu Zhu, Xinru Li, Jiayi Cao, Min Qi, Lin Zhou, Bin Su
{"title":"Microneedle Electrode Patch Modified with Graphene Oxide and Carbon Nanotubes for Continuous Uric Acid Monitoring and Diet Management in Hyperuricemia.","authors":"Ziyi Zhao, Boyu Zhu, Xinru Li, Jiayi Cao, Min Qi, Lin Zhou, Bin Su","doi":"10.1021/acsabm.4c01286","DOIUrl":"10.1021/acsabm.4c01286","url":null,"abstract":"<p><p>Hyperuricemia is a common disorder induced by purine metabolic abnormality, which will further cause chronic kidney disease, cardiovascular disease, and gout. Its main pathological characteristic is the high uric acid (UA) level in the blood, so that the detection of UA is highly important for hyperuricemia diagnosis and therapy. Herein, we report a biocompatible and minimally invasive microneedle electrode patch (MEP) for continuous UA monitoring and diet management in hyperuricemia. The composite of graphene oxide and carboxylated multiwalled carbon nanotubes was modified on the microneedle electrode surface to enhance its sensitivity, selectivity, and stability, thus realizing the continuous detection of UA in the interstitial fluid to accurately predict the UA level in the blood. This further allowed us to study the hypouricemic effect of anthocyanins on the hyperuricemia model mouse. It was found that anthocyanins extracted from blueberry can effectively inhibit the activity of xanthine oxidase to reduce the production of UA. The UA level of hyperuricemia model mice fed with anthocyanins is ∼1.7 fold lower than that of the control group. We believe that this MEP offers enormous promise for continuous UA monitoring and diet management in hyperuricemia.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"8456-8464"},"PeriodicalIF":4.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Easy-to-Engineer Flexible Nanoelectrode Sensor from an Inexpensive Overhead Projector Sheet for Sweat Neuropeptide-Y Detection.","authors":"Jayakrishnan Aerathupalathu Janardhanan, Jia-Wei She, Hsiao-Hua Yu","doi":"10.1021/acsabm.4c01229","DOIUrl":"10.1021/acsabm.4c01229","url":null,"abstract":"<p><p>In this paper, we report an inexpensive and easy-to-engineer flexible nanobiosensor electrode platform by exploring a nonconductive overhead projector (OHP) sheet for sweat Neuropeptide-Y (NPY) detection, a potential biomarker for stress, cardiovascular regulation, appetite, etc. We converted a nonconductive OHP sheet into a conductive nanobiosensor electrode platform with a hybrid polymerization method, which consists of interfacial polymerization of pyrrole and a template-free electropolymerization technique to decorate the electrode platform with poly(EDOT-COOH-<i>co</i>-EDOT-EG3) nanotubes. The selection of poly(EDOT-COOH) features an easy conjugation of NPY antibody (NPY-Ab) through EDC/Sulfo-NHS coupling chemistry, while poly(EDOT-EG3) is best known to reduce nonspecific binding of biomolecules. The antibody conjugation on the polymer surface was characterized by a quartz crystal microbalance, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and chronoamperometry techniques. The OHP nanosensor platform exhibited the successful detection of NPY analyte through a chronoamperometry method in phosphate-buffered saline with a wide range of concentrations from 1 pg/mL to 1 μg/mL with a limit of detection of 0.68 pg/mL having good linearity (<i>R</i><sup>2</sup> = 0.9841). The sensor platform exhibited excellent stability, reproducibility, repeatability, and a shelf-life of 13 days. Furthermore, the sensor showed superior selectivity to a 100 pg/mL NPY analyte among other interfering compounds such as tumor necrosis factor α, cortisol, and Interleukin-6. The clinical practicality of the sensor was confirmed through the detection of 100 pg/mL NPY spiked artificial perspiration, highlighting the possibility of integrating the sensor platform to wearable healthcare applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"8423-8433"},"PeriodicalIF":4.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}