化学•材料最新文献

{"title":"Wearable electrochemical immunosensor based on ultra-thin flexible stainless steel sheets for detection of methyl jasmonate in tomato leaves.","authors":"Yali Zhang, Xinliu Geng, Linbing Ma, Mengting Li, Xinyue Zhao, Ling Sun, Rong Tan, Lijun Sun","doi":"10.1016/j.bios.2025.117853","DOIUrl":"10.1016/j.bios.2025.117853","url":null,"abstract":"<p><p>Methyl jasmonate (MeJA), a key plant hormone, plays essential roles in plant growth, development, biotic stress responses, and wound-induced defense. Monitoring dynamic changes in MeJA in situ is vital for botanical research. Herein, coupling with paper-based analytical devices, the ultra-thin flexible stainless steel sheets with the excellent flexibility and conductivity were used to develop wearable electrochemical immunosensor for in situ and continuous detection of MeJA in plants. The ultra-thin flexible stainless steel sheets were modified with conducting carbon cement, ferrocene - graphene oxide - multi-walled carbon nanotubes composites, and MeJA antibodies to construct the wearable electrochemical immunosensor, which can detect the MeJA in the range of 10 pM-100 μM, and with a limit of detection of 5.4 pM. Using this wearable electrochemical immunosensor, the MeJA content in tomato leaves under wound stimulation was detected in situ and continuously. The results showed that MeJA levels in tomato leaves increased significantly with mechanical damage. A significant difference was observed between the untreated control group (0 cm) and the mechanically damaged group (2.0 cm), confirming the sensor's capability to monitor dynamic changes in MeJA in response to stress in real-time. In all, this study not only suggested that the ultra-thin flexible stainless steel sheets with the excellent flexibility and conductivity can be used to fabricated the wearable electrochemical sensors, but also provided a novel method for continuous in situ MeJA detection, which contributed to the understanding of MeJA regulatory mechanisms in plants and advancing precision agriculture technologies.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"117853"},"PeriodicalIF":10.5,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel thermo-activated one-pot RPA-CRISPR-Cas12b assay for Mycoplasma pneumoniae POCT.","authors":"Jiayu Feng, Ze Wu, Wenhui Zhu, Fei Jin, Minghai Zhao, Wenjie Zhong, Chen Dai, Yongjian He, Lizhi Yan, Shengquan Wu, Yuhang Wang, Yongyu Rui, Lei Zheng, Qiangqiang Fu","doi":"10.1016/j.bios.2025.117839","DOIUrl":"10.1016/j.bios.2025.117839","url":null,"abstract":"<p><p>Mycoplasma pneumoniae (M. pneumoniae), a major human respiratory pathogen, necessitates the development of rapid point-of-care testing (POCT) platforms for clinical management. However, current two-step workflows suffer from operational complexity and aerosol contamination risks. This limitation stems from CRISPR-Cas12 mediated template degradation in single-reaction systems, which compromises amplification efficiency and detection sensitivity. Here, we combined RPA and CRISPR Cas12b by leveraging the difference in their optimal temperatures to construct a novel TRACER (Thermo-activated RPA Amplification for CRISPR-Cas12b Efficient Recognition) technology. Through precise temperature modulation, TRACER sequentially executes isothermal amplification and CRISPR-mediated detection while preventing premature template cleavage, thereby maintaining optimal reaction efficiency. The platform demonstrates exceptional analytical sensitivity with a detection limit of 1 copy/μL, representing a 100-fold improvement over conventional one-pot RPA-CRISPR-Cas12a systems. Clinical validation using 195 specimens revealed diagnostic performance metrics of 99.2 % sensitivity (119/120), 100.0 % specificity (75/75), and 99.5 % accuracy (194/195). This innovative combination of single-tube reaction, field-deployable instrumentation, and cost-effectiveness establishes TRACER as an ideal POCT solution for M. pneumoniae detection in diverse clinical settings.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"117839"},"PeriodicalIF":10.5,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An artificial intelligence-assisted, kilometer-scale wireless and wearable biochemical sensing platform for monitoring of key biomarkers in urine.","authors":"Yan Dong, Wenzheng An, Yongbin Zhang, Zehao Kang, Botao Gao, Juntai Lv, Yixuan Jiang, Chang Niu, Yuling Mao, Dongzhi Zhang","doi":"10.1016/j.bios.2025.117844","DOIUrl":"10.1016/j.bios.2025.117844","url":null,"abstract":"<p><p>Wearable biochemical sensors enabling non-invasive monitoring of biomarkers in bodily fluids play a pivotal role in advancing personalized healthcare. The state-of-the-art wireless and wearable biochemical sensors still suffer from large form factors, poor detection accuracy due to sample-to-sample variation, short and weak wireless communication, and difficulty to integrate with data processing algorithm on a system level. To solve these problems, this work develops an all-range wireless and wearable biochemical sensing platform which can be integrated in a diaper for monitoring four urine biomarkers (dimethylamine, creatinine, glucose, and H⁺) with two switchable wireless modes. To simplify the circuit design and reducing the form factor of the wearable sensing platform, this work develops flexible and passive potentiometric sensing interfaces for dimethylamine and creatinine detection by developing high-performance ion-selective electrode (ISE) with customized molecularly imprinted polymers (MIPs) as ionophores. The narrowband Internet of Things (NB-IoT) far-field wireless mode enables remote, and concurrent monitoring of urine biomarkers with a working range up to tens of kilometers, while the LC resonance near-field wireless mode is capable of battery-free and intermittent detection of urine biomarkers. The wearable sensor can be easily switched between the NB-IoT far-field wireless mode and the near-field wireless mode to fit different application scenarios. The wireless sensing platform enables system level integration of the wearable biochemical sensor with a multilayer perceptron data calibration system for data auto-calibration, which reduces the errors caused by varying pH and thus improves the detection accuracy, enabling deeper AI-wearable biochemical sensor fusion for next-generation healthcare applications.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"117844"},"PeriodicalIF":10.5,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas13a-driven lateral flow assay for preamplification-free and ultrasensitive miRNA-21 detection.","authors":"Yan Zhu, Junwei Lai, Xinyao Yang, Shuqing Wang, Dayong Gu, Yan Huang, Yizhen Liu, Conghui Liu","doi":"10.1016/j.bios.2025.117850","DOIUrl":"10.1016/j.bios.2025.117850","url":null,"abstract":"<p><p>Developing a preamplification-free and sensitive clustered regularly interspaced short palindromic repeats (CRISPR)-based method is significant but still extremely challenging for microRNA (miRNA) detection. Here we present a combination of a CRISPR/Cas13a-based reaction with a lateral flow biosensor, which enables the quantitative and colorimetric readout of preamplification-free miRNA detection at room temperature. In this work, the reaction principle and the structure of the lateral flow strip are well-designed to achieve surface-enhanced Raman scattering (SERS)/colorimetric dual-signal \"turn-on\" response of target miRNA. The CRISPR/Cas13a Reporter is engineered with a DNA-RNA splicing structure to generate DNA cleavage products and reduce nonspecific collateral cleavage. Without the need for nucleic acid preamplification strategy, the developed CRISPR/Cas13a-driven lateral flow biosensor enables the microRNA-21 (miR-21) detection at room temperature with a readout time of 10 min and a total process time of less than 45 min, achieving an impressive limit of detection of 8.96 aM by SERS and 1 fM by visualization, respectively. Moreover, the platform demonstrated excellent recovery rates in spiked human serum samples. The proposed CRISPR/Cas13a-driven, dual-signal \"turn-on\"-responded lateral flow platform has the potential to simultaneously meet the requirements of convenient point-of-care visualization detection and more accurate and sensitive SERS detection of miR-21, offering a cost-effective, rapid, and reliable tool for early cancer diagnosis.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"117850"},"PeriodicalIF":10.5,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SERS-based miniaturized biosensors for alkaline phosphatase detection: Towards intelligent, real-time diagnostics in precision medicine.","authors":"Ajitesh Dhal, Ana Elena Aviña, Cheng-Jen Chang, Chang-I Chen, Tzu Sen Yang","doi":"10.1016/j.bios.2025.117793","DOIUrl":"10.1016/j.bios.2025.117793","url":null,"abstract":"<p><p>Alkaline phosphatase (ALP) is a clinically important hydrolase enzyme and a valuable biomarker for hepatobiliary diseases, metabolic bone disorders, and certain malignancies. Raman-based miniaturized sensors, particularly those employing surface-enhanced Raman scattering (SERS), have enabled ultrasensitive and selective ALP detection at femtomolar to picomolar levels in complex biological samples. This narrative review critically examines recent advances in SERS-enabled ALP sensors, highlighting hotspot engineering, nanozyme-assisted signal amplification, and microfluidic integration to achieve high-throughput, low-volume assays. It also explores the incorporation of artificial intelligence algorithms for real-time spectral interpretation and discusses the potential for integrating these systems with fifth and sixth generation (5G/6G) wireless networks for rapid, cloud-based diagnostics. In addition, this review outlines current challenges, including substrate reproducibility and standardization issues, and proposes strategies to enhance clinical translation. Collectively, these developments are transforming ALP sensing by enabling decentralized, intelligent, and personalized diagnostic platforms, which hold promise for advancing precision healthcare and improving patient outcomes.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"117793"},"PeriodicalIF":10.5,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"A CRISPR-Cas12a-mediated dual-mode luminescence and colorimetric nucleic acid biosensing platform based on upconversion nanozyme\" [Biosens. Bioelectron. 270 (2025) 116963].","authors":"Jiaxiang Yan, Bohan Yin, Qin Zhang, Chuanqi Li, Jiareng Chen, Yingying Huang, Jianhua Hao, Changqing Yi, Yu Zhang, Siu Hong Dexter Wong, Mo Yang","doi":"10.1016/j.bios.2025.117738","DOIUrl":"10.1016/j.bios.2025.117738","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117738"},"PeriodicalIF":10.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144566883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significant improvements in targeted UHPLC-ESI-MS/MS analysis of the reactive aldehydes 4-hydroxy-2(E)-nonenal and 4-hydroxy-2(E)-hexenal and application to rat serum.","authors":"S Chevolleau, C Orlandi, L Mervant, R Vuillaume, I Jouanin, N Naud, F Pierre, F Gueraud, L Debrauwer","doi":"10.1016/j.jchromb.2025.124747","DOIUrl":"10.1016/j.jchromb.2025.124747","url":null,"abstract":"<p><p>The elevated risk of colorectal cancer (CRC) induced by red or processed meat rich diets is now established. Those haem‑iron rich diets induce luminal lipid peroxidation, one of the most recognised hypotheses explaining CRC promotion. Due to their known toxic properties, quantification of reactive aldehydes such as 4-hydroxy-2(E)-nonenal (HNE) and 4-hydroxy-2(E)-hexenal (HHE) as lipid peroxidation end-products in biological fluids is of upmost importance. Following previous works on faecal waters, an UHPLC-ESI-MS/MS method has been developed and validated for HNE and HHE quantification in rat serum, using deuterated internal standards (ISs). After protein precipitation (PP) and solid phase extraction (SPE), LC-ESI-MS/MS analysis was achieved by MRM. The use of a brominated derivatisation reagent allowed using the bromine isotopes for selective detection of both HNE and HHE based on diagnostic transitions. This new method was validated according to the European Medicines Agency (EMA) guidelines. Our method proved to efficiently determine HNE and HHE serum concentrations with the required sensitivity (nM range) in serum of rats fed diets rich or not in red meat and different fatty acid compositions.</p>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1265 ","pages":"124747"},"PeriodicalIF":2.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Development of a universal one-pot CRISPR assay based on multifunctional tagged primer eliminating unstable crRNA input and PAM dependency for point-of-care detection of bacterial infections\" [Biosens. Bioelectron. 287 (2025) 117718].","authors":"Xiaofan Zhu, Jingran Jiao, Qun Ni, Li Yao, Yule Zhang, Kaiyong Liu, Lin Huang, Qingli Bo, Panzhu Qin","doi":"10.1016/j.bios.2025.117742","DOIUrl":"10.1016/j.bios.2025.117742","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117742"},"PeriodicalIF":10.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferrocene derivatives constructed Prussian blue analogs for colorimetric detection of hydrogen peroxide and glucose.","authors":"Yue Tong, Qian Liu, Dan Yang, Chunze Zhang, Yi Xiao, Mengying Shi, Meng Meng, Huixia Di, Hongmei Gao, Rimo Xi, Yongmei Yin","doi":"10.1016/j.bios.2025.117714","DOIUrl":"10.1016/j.bios.2025.117714","url":null,"abstract":"<p><p>This study reports the rational design of ferrocene-derived Prussian blue analogs (PBAs) as a new class of nanozymes with enhanced catalytic properties. Capitalizing on its high peroxidase-like activity, we developed a colorimetric assay for detecting hydrogen peroxide (H₂O₂) and glucose, which exhibited high stability, sensitivity, and accuracy in biological samples such as serum. Furthermore, by leveraging the reversible structural dynamics of Fc(COOH)₂-PBA, we engineered a dual-mode colorimetric assay for visual quantitation of H₂O₂ and glucose in complex samples, including cells, tissues, and cerebrospinal fluid. The distinct colorimetric response of this system demonstrates promising potential for point-of-care testing applications. Our findings provide mechanistic insights into the structure-activity relationships of nanozymes and will facilitate the development of application-targeted nanozymes for disease diagnostics.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117714"},"PeriodicalIF":10.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating network pharmacology and metabolomics to elucidate the mechanism of action of Yangluan formula for treating of diminished ovarian reserve.","authors":"Yang Wang, Panwei Hu, Hua Yan, Yuanyuan Wu, Ping Yin, Dongyi Shen, Xiaole Zhang, Cong Qi, Qinhua Zhang","doi":"10.1016/j.jchromb.2025.124749","DOIUrl":"10.1016/j.jchromb.2025.124749","url":null,"abstract":"<p><strong>Objectives: </strong>The Yangluan Formula (YLF) influences the outcomes of in vitro fertilization-embryo transfer (IVF-ET) in infertile women with diminished ovarian reserve (DOR); however, the potential mechanisms by which YLF ameliorates DOR have not yet been elucidated. The aim of this study was to examine the effects of YLF on IVF-ET outcomes in infertile women with DOR, and to elucidate the potential mechanisms by which YLF addresses DOR.</p><p><strong>Methods: </strong>Non-targeted metabolomics studies were conducted on follicular fluid specimens procured from individuals with DOR treated with or without YLF, and from patients with normal ovarian reserve who underwent IVF-ET treatment. Distinct metabolites were identified using untargeted metabolomics, and MetaboAnalyst was used to examine metabolic pathways. After applying network pharmacology (NP), the target of YLF acting on DOR was determined. Cytoscape software was used to develop compound-reaction-enzyme-gene networks, and molecular docking (MD) simulations were conducted to confirm the link between YLF and crucial targets.</p><p><strong>Results: </strong>Patients with DOR showed a notable reduction in the number of oocytes retrieved, incidence of 2PN fertilization, and number of cleaved embryos (P < 0.001). Additionally, the DOR cohort exhibited a markedly reduced quantity of high-quality embryos on day 3 compared to the CON cohort (P < 0.005). In contrast to the DOR cohort, the YLF cohort exhibited notably superior outcomes in terms of 2PN fertilization rates, cleavage-stage embryo development, and the number of high-grade embryos on day 3 (P < 0.05). The proportion of 2PN fertilization observed in YLF subjects substantially exceeded that in DOR individuals (81.2 % vs. 64.3 %, P < 0.05). Combined analysis of metabolomics and NP, focusing on five key targets for the action of YLF (monoamine oxidase A, monoamine oxidase B, myeloperoxidase, xanthine dehydrogenase, and phosphodiesterase 3A), four key metabolites (pelargonic acid, 1-(5-Phospho-D-ribosyl)-5-amino-4-imidazolecarboxylate, isokobusone, 5-O-(1-Carboxyvinyl)-3-phosphoshikimate), and two related pathways (glycine, serine, alanine, and threonine metabolism).</p><p><strong>Conclusion: </strong>We elucidated the mode of action of YLF in DOR treatment by integrating metabolomics and NP. YLF can effectively improve IVF outcomes in patients with DOR. This study provides new perspectives on the mechanism by which YLF improves ovarian function.</p>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1265 ","pages":"124749"},"PeriodicalIF":2.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}