化学•材料最新文献

{"title":"Detection of brain metastases from blood using Brain nanoMET sensor: Extracellular vesicles as a dynamic marker for metastatic brain tumors.","authors":"Srilakshmi Premachandran, Ishita Shreshtha, Krishnan Venkatakrishnan, Sunit Das, Bo Tan","doi":"10.1016/j.bios.2024.116968","DOIUrl":"10.1016/j.bios.2024.116968","url":null,"abstract":"<p><p>Brain metastases account for a significant number of cancer-related deaths with poor prognosis and limited treatment options. Current diagnostic methods have limitations in resolution, sensitivity, inability to differentiate between primary and metastatic brain tumors, and invasiveness. Liquid biopsy is a promising non-invasive alternative; however, current approaches have shown limited efficacy for diagnosing brain metastases due to biomarker instability and low levels of detectable tumor-specific biomarkers. This study introduces an innovative liquid biopsy technique using extracellular vesicles (EVs) as a biomarker for brain metastases, employing the Brain nanoMET sensor. The sensor was fabricated through an ultrashort femtosecond laser ablation process and provides excellent surface-enhanced Raman Scattering functionality. We developed an in vitro model of metastatic tumors to understand the tumor microenvironment and secretomes influencing brain metastases from breast and lung cancers. Molecular profiling of EVs derived from brain-seeking metastatic tumors revealed unique, brain-specific signatures, which were also validated in the peripheral circulation of brain metastasis patients. Compared to primary brain tumor EVs, we also observed an upregulation of PD-L1 marker in the metastatic EVs. A machine learning model trained on these EV molecular profiles achieved 97% sensitivity in differentiating metastatic brain cancer from primary brain cancer, with 94% accuracy in predicting the primary tissue of origin for breast metastasis and 100% accuracy for lung metastasis. The results from this pilot validation suggest that this technique holds significant potential for improving metastasis diagnosis and targeted treatment strategies for brain metastases, addressing a critical unmet need in neuro-oncology.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"116968"},"PeriodicalIF":10.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bivariate tracking of NIR phototherapeutic probe that illuminates the deterioration process of NAFLD-HCC.","authors":"Jialu Yang, Huimin Xu, Yijun Zhao, Pengju Sun, Yuanyuan Li, Ting Chen, Yanmei Zhou","doi":"10.1016/j.bios.2024.116967","DOIUrl":"10.1016/j.bios.2024.116967","url":null,"abstract":"<p><p>Non-alcoholic fatty liver disease (NAFLD) has evolved to become a major cause of hepatocellular carcinoma (HCC). Visualization of NAFLD-HCC deterioration process imaging is essential to understand the underlying pathophysiological processes. However, currently relevant probes with short emission wavelengths, univariate and the inability to achieve theranostics functionality have encountered obstacles in further evaluating the NAFLD-HCC process. Here, we present a carboxylesterase (CE)-activated NIR fluorescent probe (BODJ) which has lipid droplets (LDs)-targeting ability and emits at a wavelength of 858 nm with a fluorescence quantum yield of 19.06%. CE-activated BODJ was used as a visual tool to successfully visualize both NAFLD deterioration processes and HCC in situ based on changes in the average number of LDs and the associated fluorescence intensity fluctuations. Imaging results showed that changes associated with CE and LDs in the modelled cells varied during the transition from nonalcoholic fatty liver to nonalcoholic steatohepatitis and later progression to HCC, highlighting the close association between bivariate and disease. We also demonstrate that BODJ has photodynamic (PDT) and photothermal therapy (PTT) capabilities, allowing image-guided dual phototherapy to damage HCC in situ. This NIR probe, which takes advantage of bivariate to track the deterioration process that illuminates NAFLD-HCC and has dual phototherapy capabilities, provides new ideas for the design of probes related to the diagnosis and treatment of hepatic metabolic diseases.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"116967"},"PeriodicalIF":10.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silk fibroin-based bioelectronic devices for high-sensitivity, stable, and prolonged in vivo recording.","authors":"Huiran Yang, Ziyi Zhu, Siyuan Ni, Xueying Wang, Yanyan Nie, Chen Tao, Dujuan Zou, Wanqi Jiang, Ying Zhao, Zhitao Zhou, Liuyang Sun, Meng Li, Tiger H Tao, Keyin Liu, Xiaoling Wei","doi":"10.1016/j.bios.2024.116853","DOIUrl":"10.1016/j.bios.2024.116853","url":null,"abstract":"<p><p>Silk fibroin, recognized for its biocompatibility and modifiable properties, has significant potential in bioelectronics. Traditional silk bioelectronic devices, however, face rapid functional losses in aqueous or in vivo environments due to high water absorption of silk fibroin, which leads to expansion, structural damage, and conductive failure. In this study, we developed a novel approach by creating oriented crystallization (OC) silk fibroin through physical modification of the silk protein. This advancement enabled the fabrication of electronic interfaces for chronic biopotential recording. A pre-stretching treatment of the silk membrane allowed for tunable molecular orientation and crystallization, markedly enhancing its aqueous stability, biocompatibility, and electronic shielding capabilities. The OC devices demonstrated robust performance in sensitive detection and motion tracking of cutaneous electrical signals, long-term (over seven days) electromyographic signal acquisition in live mice with high signal-to-noise ratio (SNR >20), and accurate detection of high-frequency oscillations (HFO) in epileptic models (200-500 Hz). This work not only improves the structural and functional integrity of silk fibroin but also extends its application in durable bioelectronics and interfaces suited for long-term physiological environments.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"267 ","pages":"116853"},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field-deployable pencil lead-based electrochemical cell for the determination of the emerging contaminant and antidepressant drug venlafaxine in wastewater.","authors":"Maria Cerrato-Alvarez, Pablo Rioboó-Legaspi, Estefania Costa-Rama, M Teresa Fernández-Abedul","doi":"10.1016/j.bios.2024.116851","DOIUrl":"10.1016/j.bios.2024.116851","url":null,"abstract":"<p><p>Screening and quantification of emerging contaminants in water is of enormous relevance due to its scarcity and harmful effects on aquatic life and human health. We present a simple and cost-effective electrochemical cell for determination of the antidepressant venlafaxine, an emerging contaminant included in the EU Watch list 2022. The cell consists of pencil leads used as electrodes and a microcentrifuge tube. Modification of the working electrode with carbon nanomaterials improved the signal. Cell-related (e.g., type of pencil leads or electroactive area) as well as experimental (e.g., pH, accumulation potential and time, and scan rate) parameters were thoroughly optimized. The adsorptive nature of venlafaxine process allowed the use of an adsorptive stripping square wave voltammetry methodology to increase the sensitivity. Under optimized variables, a linear range from 0.8 to 10 μmol L^-1 with a correlation coefficient of 0.996, a sensitivity of 1.48 μmol L^-1, a LOD of 0.4 μmol L^-1 and a RSD of 2.4 % were achieved. Selectivity was also studied, especially with respect to the main metabolite, o-desmethylvenlafaxine. The methodology distinguishes its signal from that of the main compound, allowing its determination. A similar linear range was obtained for the metabolite, with a LOD of 0.6 μmol L^-1. The platform developed was applied for venlafaxine quantification in spiked wastewaters from the Febros plant in Portugal, obtaining satisfactory recoveries. Furthermore, the versatility of pencil leads made it possible to combine them with modified paper for sampling and buffering in order to decentralize the determination, showing promising results.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"267 ","pages":"116851"},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saline based microfluidic soft pressure sensor utilizing a three-dimensional focused electric field for motion and healthcare monitoring.","authors":"Suhyeon Kim, Hyeonsu Woo, Seungbin Yoon, HyungGon Shin, Keehoon Kim, Geonhwee Kim, Geunbae Lim","doi":"10.1016/j.bios.2024.116868","DOIUrl":"10.1016/j.bios.2024.116868","url":null,"abstract":"<p><p>This paper introduces the 'Spatially Focused Saline-based Pressure Sensor (SF-SaPS)', a novel soft microfluidic pressure sensor featuring a distinctive three-dimensional focusing structure. By critically reducing the cross-sectional area of the microchannel at the focused structure, the SF-SaPS achieves excellent sensitivity to pressure within the sensing region. With the spatially focused region, the SF-SaPS could detect a wide range of pressure from gentle touches to human weight, which is typically unachievable with low-conductivity sensing media such as saline, a medium inherently safe for human use. Beyond its sensitivity, the SF-SaPS exhibits sensing performance and stability comparable with conventional liquid metal-based pressure sensors. Our sensor demonstrated minimal signal drift, a rapid response time of 70 ms under cyclic loading, and 20-day long-term stability tests immersed in water. Additionally, the sensor possesses a transparency advantage unattainable by liquid metal sensors as we utilized transparent polymers and saline. A unique advantage of the SF-SaPS lies in its selective spatial and mechanical sensitivity; as the electrical resistance is highly dependent on changes in the cross-sectional area of the microchannels, the sensor has superior pressure sensitivity compared to bending and strain. Finally, various application examples highlight the SF-SaPS's advantages. By configuring the sensor in a two-axis array, the SF-SaPS facilitates pressure mapping across a plane. Additionally, it proves effective in healthcare monitoring, from radial pulse to finger movements. In conclusion, the SF-SaPS's combination of performance, stability, biocompatibility, and transparency positions this sensor as a versatile tool for applications extending beyond healthcare, as demonstrated in this study.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"267 ","pages":"116868"},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aggregation-induced emission luminogens for intracellular bacteria imaging and elimination.","authors":"Mingji Jiang, Jing Kang, Alideertu Dong","doi":"10.1016/j.bios.2024.116873","DOIUrl":"10.1016/j.bios.2024.116873","url":null,"abstract":"<p><p>Intracellular bacterial infections are a serious threat to human health due to their ability to escape immunity and develop drug resistance. Recent attention has been devoted to identifying and ablating intracellular bacteria with fluorescence probes. Aggregation-induced emission luminogens (AIEgens) photosensitizers as fluorescence probes possess excellent photostability and rapid response, which have emerged as powerful fluorescent tools for intracellular bacterial detection and antibacterial therapy. This review is intended to highlight the current advances in AIEgens on intracellular bacteria imaging and elimination, which covers topics from intracellular AIE mechanism, intracellular bacteria imaging of AIEgens to the elimination of intracellular bacteria with AIEgens. AIEgens utilized different interactions to detect intracellular bacteria, emitting bright light due to restricted intramolecular movement to visualize intracellular bacteria. Photosensitive AIEgens generate reactive oxygen species (ROS) in the aggregate state to elimate intracellular bacteria. Moreover, the prospects and application of AIEgens in intracellular bacteria imaging and elimination are also discussed, which provides insights for the development of AIE-based diagnostic and therapeutic materials and technologies.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"267 ","pages":"116873"},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Albendazole nanosuspension coated granules for the rapid localized release and treatment of colorectal cancer.","authors":"Yi Guo, Henis J Patel, Akanksha S Patel, Emilio Squillante, Ketan Patel","doi":"10.1016/j.colsurfb.2024.114320","DOIUrl":"10.1016/j.colsurfb.2024.114320","url":null,"abstract":"<p><p>Albendazole (ABZ), an anthelmintic drug, has been repurposed to treat various types of cancers. However, poor solubility of ABZ, resulting in low bioavailability, limits its application. Nanosuspension is a versatile method for enhancing the dissolution of hydrophobic molecules, but a successful drying has been the biggest challenge in the field. The objective of this research is to formulate and optimize ABZ nanosuspension (NS) coated granules for rapid delivery of ABZ for the treatment of colorectal cancer. ABZ NS was prepared by dual centrifugation method using Kollidon® VA64 and sodium lauryl sulphate (SLS) as stabilizers. The processing method was optimized to obtain a stable nanosuspension with particle size < 300 nm. The optimized ABZ NS was coated on microcrystalline cellulose (MCC) to form the nano-coated granules (NCG) and filled in EUDRACAP® for colon targeted delivery. The ABZ NS and NCG achieved ∼ 60 % and ∼55 % drug release, respectively, in presence of bile salt at colonic pH. Half-maximal inhibitory concentration (IC₅₀) of ABZ NS was found to be 1.18 ± 0.081 µM and 3.59 ± 0.080 µM in two colorectal cancer cell lines: HCT 116 and HT-29, respectively. In addition, In vitro 3D tumor assay revealed that ABZ NS has superior tumor growth inhibition activity compared to the control and pure ABZ. The preparation of ABZ NCG in EUDRACAP® could be a promising approach to achieve colon targeted delivery and to repurpose ABZ for the treatment of colorectal cancer.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114320"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redox-responsive degradation of antimicrobials with programmable drug release for enhanced antibacterial activity.","authors":"Yue Zhang, Xuehan Yang, Yawei Zhao, Fangman Chen, Tongfei Shi, Ziping Wu, Xuenian Chen, Ming Zhang, Li Chen","doi":"10.1016/j.colsurfb.2024.114308","DOIUrl":"10.1016/j.colsurfb.2024.114308","url":null,"abstract":"<p><p>The global crisis of antibiotic resistance has impelled the exigency to develop more effective drug delivery systems for the treatment of bacterial infection. The development of possessing high biocompatibility and targeted delivery of antimicrobials remains a persisting challenge. For programmable release of efficient antimicrobials in infection sites to enhance antibacterial activity, herein, we fabricated diselenide-bridged mesoporous organosilica nanoparticle-supported silver nanoparticles (Ag NPs) with high drug-loading capacity for the co-delivery of tobramycin (TOB) within one drug delivery system (Ag-MON@TOB (Se)). The resultant Ag-MON@TOB (Se) exhibited favorable biocompatibility due to its high stability in the physiological condition. Notably, such Ag-MON@TOB (Se) manifested a programmable structural destabilization to trigger sequential drug release in response to the oxidative stimuli within the bacterial infection microenvironment. In contradistinction to the oxidation-stable disulfide bond moieties within the framework of the nanocarrier (Ag-MON@TOB (S)), the Ag-MON@TOB (Se) with its programmed drug release behavior augmented prominent antibacterial therapy both in vitro and in vivo. This work represents a promising strategy for programmable drug release by harnessing a responsive degradable vehicle to enhance the treatment of bacterial infection.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114308"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional mesoporous nanoselenium delivery of metformin breaks the vicious cycle of neuroinflammation and ROS, promotes microglia regulation and alleviates Alzheimer's disease.","authors":"Xian Guo, Borui Zhang, Yutong Chen, Zhi Jia, Xiaoyu Yuan, Li Zhang, Jie Liu, Yanan Liu","doi":"10.1016/j.colsurfb.2024.114300","DOIUrl":"10.1016/j.colsurfb.2024.114300","url":null,"abstract":"<p><p>Clinical trials based on a single molecular target continue to fail, and the adverse effects of Aβ protein aggregation and neuroinflammation need to be solved and treatment of Alzheimer's disease. Herein, by designed a nano-sized flower mesoporous selenium transport carrier (Met@MSe@Tf) with high enzyme-like activity, metformin (Met) was loaded, and transferrin (Tf) was modified to bind to transferrin receptor to promote receptor-mediated transport across the BBB. In the AD lesion environment, with the acidic environment response dissociation, promote the release of metformin by nanoflower to achieve therapeutic effect in the brain lesion site. Metformin, a major anti-diabetic drug in diabetic metabolism, has been found to be a promising new therapeutic target in neurodegenerative diseases. Further studies showed that the metformin drug release from the designed and synthesized transport nanoparticles showed high intrinsic activity and the ability to degrade the substrate involved, especially the degradation of Aβ deposition in the cortex and hippocampus, increased the phagocytosis of microglia, thus relieving neuroinflammation simultaneously. Collectively, in vivo experiments demonstrated that Met@MSe@Tf significantly increased the number of NeuN-positive neurons in the hippocampus of AD mice, promoted neurovascular normalization in the brain, and improved cognitive dysfunction in AD transgenic AD mice. Thus, it provides a preclinical proof of concept for the construction of a highly modular accurate drug delivery platform for Alzheimer's disease.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114300"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional NIR-II nanoplatform for disrupting biofilm and promoting infected wound healing.","authors":"Jinqiang Wu, Xiaolei Huo, Jinjia Liu, Fanqiang Bu, Pengfei Zhang","doi":"10.1016/j.colsurfb.2024.114330","DOIUrl":"10.1016/j.colsurfb.2024.114330","url":null,"abstract":"<p><p>Healing wounds presents a significant challenge due to bacterial biofilm infections and the inherent drug resistance of these biofilms. This report introduces a multifunctional nanoplatform (NPs) designed to combat wound biofilm infections using NIR-II photothermal therapy. The NPs are self-assembled from amphiphilic polymers (AP) to encapsulate photothermal polymers (PT) through classic electrostatic interactions. Importantly, these NPs are electrically neutral, which enhances their ability to penetrate biofilms effectively. Once inside the biofilm, the NPs achieve complete thermal ablation of the biofilm under NIR-II laser irradiation. Additionally, when exposed to laser and the GSH microenvironment, the NPs exhibit strong photothermal effects and self-degradation capabilities. In vitro tests confirm that the NPs have excellent antibacterial and anti-biofilm properties against methicillin-resistant Staphylococcus aureus (MRSA). In vivo studies demonstrate that the NPs can efficiently clear wound biofilm infections and promote wound healing. Notably, the NPs show superior photothermal effects under NIR-II laser irradiation compared to NIR-I lasers. In summary, the developed NPs serve as an integrated diagnostic and therapeutic nano-antimicrobial agent, offering promising applications for biofilm wound infections and wound healing.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114330"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}