化学•材料最新文献

{"title":"Magnetic assembly of microwires on a flexible substrate for minimally invasive electrophysiological recording.","authors":"Claire King Teck Sieng, Chan Jun Yi, Taiki Yasui, Koji Yamashita, Rioki Sanda, Kensei Sakamoto, Yuki Kondo, Ko Suzuki, Shinnosuke Idogawa, Yu Seikoba, Rika Numano, Kowa Koida, Takeshi Kawano","doi":"10.1016/j.bios.2024.116927","DOIUrl":"10.1016/j.bios.2024.116927","url":null,"abstract":"<p><p>Understanding the neural system in the brain requires the detection of signals from the tissue. Microscale electrodes enable high spatiotemporal neural recording, whereas traditional microelectrodes cause material and geometry mismatches between the electrode and the tissue, leading to injury and signal loss during recording. In this study, we propose a fabrication technique that uses magnetic force to facilitate assembly of vertical microscale wire-electrodes on a flexible substrate. Two-channel 15-μm-diameter and 400-μm-length nickel-microwire electrodes on a 5-μm-thick flexible parylene film are designed and fabricated. Impedance characteristics of these electrodes are <500 kΩ at 1 kHz, with output/input signal amplitude ratios of over 90%. In vivo neural recording in mice demonstrates that both local field potentials and action potentials are detected through each wire electrode, confirming the minimal invasiveness during the electrode penetration and through immunohistochemical tissue analysis.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"116927"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron-responsive nanoparticle-loaded bilayer dissolving microneedles for selective and controlled transdermal delivery of deferasirox in β-thalassemia major treatment.","authors":"Andi Dian Permana, Sitti Nurkhadijah Maharani, Anugerah Yaumil Ramadhani Aziz, Indianty Dwi Ramadhany, Achmad Himawan, Habibie, Rangga Meidianto Asri, Muhammad Nur Amir, Rina Masadah","doi":"10.1016/j.colsurfb.2024.114416","DOIUrl":"10.1016/j.colsurfb.2024.114416","url":null,"abstract":"<p><p>Deferasirox (DFX) is widely used to manage β-thalassemia major (β-TM), but its oral administration is limited by low bioavailability and side effects. To address these challenges, we developed iron-responsive nanoparticles (NP-IR) of DFX using ferrocene as the iron-responsive material, incorporated into dissolving microneedles (DMN) for transdermal delivery. The NP-IR measured 276.67 ± 7.80 nm with an entrapment efficiency of 47.54 ± 3.68 %. FTIR analysis confirmed DFX incorporation, while reduced crystallinity suggested enhanced formulation. In vitro testing demonstrated controlled DFX release in the presence of iron, highlighting its targeted responsiveness. The DMN containing NP-IR, composed of polyvinyl pyrrolidone and polyvinyl alcohol, showed less than 10 % height reduction and successfully penetrated the fourth layer of Parafilm®, simulating human skin penetration. Ex vivo studies validated effective DFX delivery through rat skin with high iron selectivity, while in vivo experiments in an iron overload rat model revealed sustained, controlled release, outperforming oral administration and potentially ehancing DFX efficacy in β-TM treatment.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114416"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical fiber SPR Pb²⁺sensor with tip-printed Fabry-Perot interferometer for temperature compensation.","authors":"Lu-Feng Wang, Ya-Nan Zhang, Mao-Qing Chen, Yu Wang, Pengqi Gong, Yong Zhao","doi":"10.1016/j.bios.2024.117013","DOIUrl":"10.1016/j.bios.2024.117013","url":null,"abstract":"<p><p>Due to the serious biological toxicity and environmental refractory of heavy metal ions, the detection of heavy metal ions in liquids has attracted great attention. A novel fiber optic surface plasmon resonance (SPR) sensor is presented for detecting lead ions (Pb²⁺) with temperature compensation. The sensitivity of SPR channel to Pb²⁺ is up to -41.55 nm/μM and the detection limit is 7.05 nM after three parallel experiments by synthesizing Schiff base and fixing it on the sensor surface. Using femtosecond laser-induced two-photon polymerization (TPP) technology, a compact fiber-tip miniature Fabry-Perot interferometer (FPI) was printed at the end of the fiber-optic SPR sensor, thus realizing the temperature measurement in the process of detecting Pb²⁺. The FPI channel exhibits a linear response to temperature and remains unaffected by Pb²⁺ concentration, thus facilitating temperature correction. The experimental results also show that the proposed sensor has the advantages of compact structure, easy to prepare, good stability, specificity and repeatability. Therefore, this kind of sensor has great potential in various biochemical test applications, and opens up a new way to prepare compact and flexible optical sensors.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117013"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical methods for evaluation of therapeutic monoclonal antibodies: A review.","authors":"Diana R Cunha, Marcela A Segundo, M Beatriz Quinaz","doi":"10.1016/j.bios.2024.116988","DOIUrl":"10.1016/j.bios.2024.116988","url":null,"abstract":"<p><p>Biopharmaceuticals are complex pharmaceutical drug products produced by biotechnology in living systems. Small changes in the production process can induce differences in the structure of the active ingredient, which may have a strong impact on its pharmacological properties. Therefore, quality assurance of biopharmaceuticals results in a high analytical effort. Strict quality and stability monitoring of potentially critical quality attributes (CQAs) is required. Electrochemical methods have been contributing to the expansion of sensors and biosensors due to their advantages, such as cost-effectiveness and easy operation. Here, we discuss the recent developments in sensors and biosensors using electrochemical techniques employed for the determination of biopharmaceuticals, namely monoclonal antibodies (mAb) and fragments of mAbs. In the frame of this information, this review aims to critically address electrochemical sensors and biosensors for the analysis of biopharmaceuticals reported since 2016. Electrochemical bio(sensors) development has been mainly based on gold and aptamers, respectively, as the most used electrode material and biorecognition element. Also, Bevacizumab (BEVA) was the main therapeutic mAb detected and 69% of the works described a (bio)sensor) that can be applied to therapeutic drug monitoring.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"116988"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinspired synthesis of virus-like particle-templated thin silica-layered nanocages with enhanced biocompatibility and cellular uptake as drug delivery carriers.","authors":"Kyeong Rok Kim, Ae Sol Lee, Hye Ryoung Heo, So-Young Park, Chang Sup Kim","doi":"10.1016/j.colsurfb.2024.114418","DOIUrl":"10.1016/j.colsurfb.2024.114418","url":null,"abstract":"<p><p>The bioinspired synthesis of virus-like silica nanoparticles in biomedical applications makes it possible to utilize the cellular delivery capabilities of viruses while minimizing the cytotoxicity of inorganic silica. In this study, we developed a diatom-inspired method for synthesizing silica-layered nanocages utilizing R5 peptide-functionalized virus-like particles (VLPs). R5 peptides were genetically inserted into the F-G loop of human papillomavirus 16 L1 proteins (HPV16 L1-R5). HPV16 L1-R5 was self-assembled into VLPs under an acidic pH similar to native ones and exhibited ∼65 % drug encapsulation efficiency. The HPV16 L1-R5 VLP@silica nanocages (SiNPs) were synthesized through diatom-inspired silicification of HPV16 L1-R5 VLPs via intermolecular interaction of the R5 peptide and polyol. HPV16L1-R5 VLP@SiNPs displayed uniform, monodisperse particles with approximately 10 nm silica layer compared to HPV16 L1-R5 VLPs. HPV16 L1-R5 VLP@SiNPs showed high biocompatibility at high concentrations, unlike commercial mesoporous SiNPs. Furthermore, the virus-like HPV16 L1-R5 VLP@SiNPs resulted in approximately 2.5-fold increased cellular uptake efficiency compared to commercial mesoporous SiNPs. These results suggest that the thin silica layer on HPV16 L1-R5 VLPs retains cellular delivery capacity while reducing cytotoxicity. Our strategy presents an innovative method for synthesizing virus-like nanoparticles in biomedical applications, enhancing cellular delivery capacity and biocompatibility.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114418"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The biological macromolecules constructed Matrigel for cultured organoids in biomedical and tissue engineering.","authors":"Ke-Yu Zhao, Yi-Xiang Du, Hui-Min Cao, Li-Ya Su, Xiu-Lan Su, Xian Li","doi":"10.1016/j.colsurfb.2024.114435","DOIUrl":"10.1016/j.colsurfb.2024.114435","url":null,"abstract":"<p><p>Matrigel is the most commonly used matrix for 3D organoid cultures. Research on the biomaterial basis of Matrigel for organoid cultures is a highly challenging field. Currently, many studies focus on Matrigel-based biological macromolecules or combinations to construct natural Matrigel and synthetic hydrogel scaffolds based on collagen, peptides, polysaccharides, microbial transglutaminase, DNA supramolecules, and polymers for organoid culture. In this review, we discuss the limitations of both natural and synthetic Matrigel, and describe alternative scaffolds that have been employed for organoid cultures. The patient-derived organoids were constructed in different cancer types and limitations of animal-derived organoids based on the hydrogel or Matrigel. The constructed techniques utilizing 3D bioprinting platforms, air-liquid interface (ALI) culture, microfluidic culture, and organ-on-a-chip platform are summarized. Given the potential of organoids for a wide range of therapeutic, tissue engineering and pharmaceutical applications, it is indeed imperative to develop defined and customized hydrogels in addition to Matrigel.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114435"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visual and fluorescence dual mode platform for sensitive and accurate screening of breast carcinoma.","authors":"Qiongdan Zhang, Qingyi Liu, Kang Long, Kang Zhou, Zheng Yang, Anqi Ge, Jinhui Hu, Caiyun Peng, Wei Wang, Huizhen Wang, Bin Li","doi":"10.1016/j.bios.2024.117047","DOIUrl":"10.1016/j.bios.2024.117047","url":null,"abstract":"<p><p>Compared to single-mode detection, dual-mode sensing strategies have garnered increasing attention from researchers due to their superior detection accuracy and reliability. Exosomes, as non-invasive biomarkers, hold significant potential for disease diagnosis. However, sensitive and precise detection of exosomes still presents considerable technical challenges. Inspired by the advantages of dual-mode detection, we developed a visual and fluorescence dual-mode platform (VFDMP) based on an aptamer strategy for exosome detection using enzyme-free nucleic acid amplification and nanomaterial-assisted cation exchange reactions (CERs). The Aptamer-ssDNA complexes capture tumor-derived exosomes, releasing abundant single-stranded DNA (ssDNAs), which then triggers the catalytic hairpin assembly (CHA) cycle, leading to the release of Ag⁺. The introduced CdTe quantum dots (QDs) act as signal reporters, interacting with Ag⁺ through CERs, and switching both fluorescence and visual signals from \"on\" to \"off\" to achieve exosome detection. Based on this innovative sensing principle, the developed FL/visual dual-mode aptasensor demonstrated excellent sensitivity and accuracy, achieving a low detection limit of 1.1 particles/μL by fluorometer, while exosome concentrations as low as 300 particles/mL could be visually distinguished by naked eye. Furthermore, this dual-mode platform can directly detect exosomes in clinical human serum samples, with only a small volume (10 μL) required. It can accurately differentiate between healthy individuals and breast cancer patients, as well as identify cancer stages (Stage II and Stage III) and subtypes (triple-negative, luminal B, and HER2+). These results suggest that the developed dual-mode detection strategy holds great promise as a sensitive, accurate method for biomarker analysis in clinical samples.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117047"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescence imaging opens a new window for the diagnosis of early-stage Alzheimer's disease.","authors":"Yi-Xuan Fu, Shi-Yu Liu, Wu-Yingzheng Guo, Long-Can Mei, Yi-Jie Dai, Xuan-Jian Peng, Jun Yin, Da-Wei Wang, Guang-Fu Yang","doi":"10.1016/j.bios.2024.117051","DOIUrl":"10.1016/j.bios.2024.117051","url":null,"abstract":"<p><p>As the global population ages, the incidence and prevalence of Alzheimer's disease (AD) continues to rise, posing a serious threat to human health. Butyrylcholinesterase (BChE), which is overexpressed in the brains of patients with AD, is a potential drug target and biomarker. However, the molecular mechanism underlying BChE's role in the AD process remains unclear. Therefore, the development of tools for BChE detection can aid in the diagnosis of AD and deepen our understanding of BChE's contribution to disease progression. Motivated by a bioinspired strategy based on the natural substrate of BChE, we designed a BChE fluorescent probe (HCYO) with a novel recognition group for BChE detection to assist in the early diagnosis of AD. This probe can selectively detect endogenous BChE with an excellent detection limit of 28.9 ng/mL. Using HCYO, we successfully imaged four-week-old mice with an ultraearly AD model, the early diagnosis of the disease. Furthermore, using this HCYO probe, we confirmed that BChE influences the inflammation-induced upregulation the levels of phosphorylated tau and Trigger Receptor Expressed on Myeloid Cells 2, impacting AD progression. These findings provide a crucial theoretical basis for the development of BChE inhibitors for AD treatment.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117051"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced transcytosis and therapeutic efficacy of paclitaxel nanoparticles: Pyridylboronic acid modification and sialic acid targeting.","authors":"Manzhen Li, Miao Chen, Pengxin Li, Ziqi Zhang, Han Yu, Xiangtao Wang","doi":"10.1016/j.colsurfb.2024.114417","DOIUrl":"10.1016/j.colsurfb.2024.114417","url":null,"abstract":"<p><p>Efficient drug delivery and deeper penetration into tumors have become a primary focus of anti-tumor nanomedicine. In this study, pyridylboronic acid (BPA), as a targeting ligand for sialic acid, which is highly expressed on the surface of tumor cells, was conjugated with DSPE-PEG2k-NH₂ to synthesize DSPE-PEG2k-BPA and used to encapsulate PTX. The resultant PTX@DSPE-PEG2k-BPA nanoparticles (DPB NPs) showed a mean particle size of 189.0 ± 3.5 nm, with a high drug loading content of 48.75 % and a rod-like morphology. In contrast to PTX@DSPE-mPEG2k nanoparticles (DP NPs), DPB NPs displayed enhanced cellular uptake and targetability to 4T1 tumor cells. Interestingly, BPA modification could also enhance transcytosis through the endoplasmic reticulum-Golgi pathway, thus improving penetration and accumulation of nanoparticles in tumors. An in vivo study on 4T1 tumor-bearing mice demonstrated that DPB NPs achieved a faster and more accumulation in tumors than DP NPs after intravenous administration, led to significantly improved therapeutic efficacy with a higher tumor inhibition rate (74.27 % vs 50.58 %, p < 0.01). In conclusion, the modification of BPA presents a strategy for the development of drug delivery systems that exhibit dual functionalities: active targeting and transcytosis.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114417"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications of DNA functionalized gold nanozymes in biosensing.","authors":"Min Yang, Ran Wang, Yushi Xie, Longjiao Zhu, Jiaqiang Huang, Wentao Xu","doi":"10.1016/j.bios.2024.116987","DOIUrl":"10.1016/j.bios.2024.116987","url":null,"abstract":"<p><p>In recent years, nanozymes have emerged as highly potential substitutes, surpassing the performance of natural enzymes. Among them, gold nanoparticles (AuNPs) and their metal hybrids have become a hot topic in nanozyme research due to their facile synthesis, easy surface modification, high stability, and excellent enzymatic activity. The integration of DNA with AuNPs, by precisely controlling the assembly, arrangement, and functionalization of nanoparticles, greatly facilitates the development of highly sensitive and selective biosensors. This review comprehensively elaborates on three core strategies for the combination of DNA with AuNPs, and deeply analyzes two widely applied enzyme activities in the field of sensing technology and the catalytic principles behind them. On this basis, we systematically summarize various methods for regulating the activity of gold nanozymes by DNA. Following that, we comprehensively review the latest research trends of DNA-Au nanozymes in the field of biosensing, with a particular focus on several crucial application areas such as food safety, environmental monitoring, and disease diagnosis. In the conclusion of the article, we not only discuss the main challenges faced in current research but also look forward to potential future research directions.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"116987"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}