Biosensors and Bioelectronics最新文献

A disposable VHH-based SPR fiber probe for sensitive and specific detection of MMP-9 in cancer and inflammatory disease 一种基于vhh的一次性SPR纤维探针，用于敏感和特异性检测癌症和炎症性疾病中的MMP-9

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-09 DOI: 10.1016/j.bios.2025.117684

Dandan Wu , Jian Yang , Junxiao Cong , Jian Zhou , Youbin Li , Feng Yan , Xuejin Li , Yuzhi Chen , Liang Ye

{"title":"A disposable VHH-based SPR fiber probe for sensitive and specific detection of MMP-9 in cancer and inflammatory disease","authors":"Dandan Wu , Jian Yang , Junxiao Cong , Jian Zhou , Youbin Li , Feng Yan , Xuejin Li , Yuzhi Chen , Liang Ye","doi":"10.1016/j.bios.2025.117684","DOIUrl":"10.1016/j.bios.2025.117684","url":null,"abstract":"<div><div>A novel one-step label-free immunoassay platform was developed through the integration of nanobody (VHH) technology with a disposable surface plasmon resonance (SPR) fiber probe for rapid, precise biomarker detection. The platform employs immobilization of high-affinity VHH against human matrix metalloproteinase-9 (MMP-9), a key biomarker in cancer and inflammatory bowel disease (IBD), on gold-coated optical fiber surfaces. This disposable VHH-based SPR fiber probe enables sensitive MMP-9 quantification in various cancer cell lines as well as tissue and serum specimens from murine models of colorectal and breast cancer and dextran sulfate sodium (DSS)-induced colitis. Clinical validation using human samples demonstrated successful MMP-9 detection in breast cancer and IBD cohorts. The VHH-based SPR fiber probe demonstrated rapid, sensitive, and specific MMP-9 detection with minimal cross-reactivity, establishing its reliability for clinical applications in early cancer and IBD diagnosis, therapeutic monitoring, and prognostic assessment.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117684"},"PeriodicalIF":10.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TiO2/WO3 nanoarrays photodetector and liposomes equipped with electron donor-mediated signal amplification for immunoassay applications 二氧化钛/WO3纳米阵列光电探测器和脂质体配备电子供体介导的信号放大免疫分析应用

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-07 DOI: 10.1016/j.bios.2025.117676

Shanghua Liu , Yueyuan Li , Feng Jiang , Mingxiang Wang , Shiyi Hou , Yueyun Li , Qin Wei

{"title":"TiO2/WO3 nanoarrays photodetector and liposomes equipped with electron donor-mediated signal amplification for immunoassay applications","authors":"Shanghua Liu , Yueyuan Li , Feng Jiang , Mingxiang Wang , Shiyi Hou , Yueyun Li , Qin Wei","doi":"10.1016/j.bios.2025.117676","DOIUrl":"10.1016/j.bios.2025.117676","url":null,"abstract":"<div><div>Exploring efficient photoelectric conversion platforms and innovative sensing strategies is the driving force of sensors to realize sensitivity detection. In this work, a photoelectrochemical (PEC) sensor integrating the type-II heterojunction of TiO<sub>2</sub>/WO<sub>3</sub> nanoarrays (NAs) composites and liposome-mediated electron donor equipping strategy, was present for highly sensitive immunoassays. TiO<sub>2</sub>/WO<sub>3</sub> NAs with the well-ordered density orientation, stable signal output, and minimal variation between batches, were an excellent PEC sensing substrate for the analysis of low abundance disease-related markers. A complete chain of evidence confirmed the successful charge carriers transfer containing density functional theory (DFT), <em>in situ</em> XPS (ISI-XPS), and electron paramagnetic resonance (EPR). Impressively, liposome-encapsulated electron donor (ascorbic acid) was utilized as a signal amplification strategy, which effectively shortened the distance between the electron donor and the substrate material, improved the photoelectronic conductivity of the photoelectric sensing interface. Tumor Necrosis Factor-Like Ligand 1A (TL1A) as a new target for inflammatory bowel disease to test the feasibility of this \"signal-on\" immunosensor in the linear range from 10.00 pg/mL to 100.00 ng/mL. The sensing model is only a small demonstration of its application potential, and we also expect that this will be a promising path to broaden the analysis of other targets.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117676"},"PeriodicalIF":10.7,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic monitoring of skin barrier function enabled by 3D bioelectronic human skin model 三维生物电子人体皮肤模型实现皮肤屏障功能动态监测

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-06 DOI: 10.1016/j.bios.2025.117673

Sihui Xu , Kunal Das Mahapatra , Nicolas Maïno , Onur Parlak

{"title":"Dynamic monitoring of skin barrier function enabled by 3D bioelectronic human skin model","authors":"Sihui Xu , Kunal Das Mahapatra , Nicolas Maïno , Onur Parlak","doi":"10.1016/j.bios.2025.117673","DOIUrl":"10.1016/j.bios.2025.117673","url":null,"abstract":"<div><div>The complexity of human skin poses significant challenges in replicating its structure and function for applications such as drug testing, disease modeling, and biosensing. Existing models, like 2D cell cultures, are unable to replicate the layered structure and barrier properties of the skin, while animal studies raise ethical concerns and are costly. Here we present a 3D epidermal skin model, designed to replicate the multilayered architecture of human skin, and integrated with organic electrochemical transistors (OECTs) for real-time non-invasive monitoring of skin barrier integrity. The model represented herein enables the study of both structural and functional aspects of human skin. By interfacing with OECTs, we can dynamically assess skin barrier function, demonstrating higher sensitivity and temporal resolution compared to traditional methods like transepithelial electrical resistance (TEER). This innovative approach merges 3D skin models with advanced bioelectronic technologies, offering a powerful tool for skin research that adheres to ethical standards while offering valuable insights into skin physiology.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117673"},"PeriodicalIF":10.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144242028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A miniaturized liver function detection system with machine learning enhancing strategy 具有机器学习增强策略的小型化肝功能检测系统

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-06 DOI: 10.1016/j.bios.2025.117667

Yang Zeng , Bianzheng Wang , Jie Cheng , Chunhui Ren , Jinhong Guo , Renting Liu , Shan Liu , Jiuchuan Guo

{"title":"A miniaturized liver function detection system with machine learning enhancing strategy","authors":"Yang Zeng , Bianzheng Wang , Jie Cheng , Chunhui Ren , Jinhong Guo , Renting Liu , Shan Liu , Jiuchuan Guo","doi":"10.1016/j.bios.2025.117667","DOIUrl":"10.1016/j.bios.2025.117667","url":null,"abstract":"<div><div>Serum alanine aminotransferase (ALT) is one of the most sensitive indicators of liver function and is crucial in diagnosing acute liver injury (ALI). However, its widespread clinical application is limited due to expensive equipment, detection delays, and high technical requirements, especially in resource-limited areas. This paper presents a miniaturized liver function detection system with machine learning enhancing strategy to address these challenges. The system integrates a quantitative detection algorithm based on grayscale processing and a semi-quantitative detection algorithm based on a convolutional neural network (CNN). The CNN model consists of four block units, each comprising a convolutional layer, an activation function, and a pooling layer. The model was validated using the hold-out method. Additionally, the detection instrument employs rapid and stable temperature control at 37 ± 0.4 °C to maintain serum enzyme activity. The system achieves a quantitative limit of detection of 5.47 U/L, with a measurable range of 6∼395 U/L. The semi-quantitative classification intervals are <1 × ULN (Upper limit of normal), 1–5 × ULN, and >5 × ULN. Accurate ALT concentration results can be obtained within 3 min. Compared to results from a fully automated biochemical analyzer, the system exhibits a linear correlation coefficient of 0.9930 for quantitative detection and an accuracy of 96.97 % for semi-quantitative detection. The impact of common interfering substances is less than 8 %, and the coefficient of variation (CV) is consistently below 10 %, demonstrating good reliability. This system offers a low-cost, fast, and accurate solution for liver function detection, providing a convenient and efficient alternative for clinical applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117667"},"PeriodicalIF":10.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-driven 3D-QSAR models facilitated rapid on-site broad-spectrum immunoassay of (fluoro)quinolones using evanescent wave fiber-embedded optofluidic biochip 机器学习驱动的3D-QSAR模型使用隐波光纤嵌入的光流生物芯片促进了（氟）喹诺酮类药物的快速现场广谱免疫分析

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-05 DOI: 10.1016/j.bios.2025.117666

Yuxin Zhuo , Siyan Liu , Wenjuan Xu , Yunsong Mu , Anna Zhu , Feng Long

{"title":"Machine learning-driven 3D-QSAR models facilitated rapid on-site broad-spectrum immunoassay of (fluoro)quinolones using evanescent wave fiber-embedded optofluidic biochip","authors":"Yuxin Zhuo , Siyan Liu , Wenjuan Xu , Yunsong Mu , Anna Zhu , Feng Long","doi":"10.1016/j.bios.2025.117666","DOIUrl":"10.1016/j.bios.2025.117666","url":null,"abstract":"<div><div>(Fluoro)quinolones (FQs) pose significant threats to public health due to their widespread use and persistence in food and water sources. Given the extensive variety of FQs, testing each compound individually is prohibitively expensive and time-consuming. Here, we introduce an evanescent wave fiber-embedded 3D optofluidic biochip (e-FOB) that enables rapid, on-site detection of 14 FQs through a broad-spectrum immunoassay. The e-FOB integrates a functionalized fiber biosensor with a 3D optofluidic chip, leveraging a broad-spectrum anti-FQ antibody to achieve high sensitivity and specificity. Limits of detection for all tested FQs were below 3.0 μg/L, with excellent reusability and stability demonstrated over 400 cycles. Two machine learning-driven 3D quantitative structure-activity relationship (ML-3D-QSAR) models were developed to identify key physicochemical factors and quantitative interactions influencing FQs detection performance. The simulation results of both models demonstrate that the e-FOB, leveraging broad-spectrum antibodies, enables the detection of an entire class of FQs, highlighting the potential for broad-spectrum detection of antibiotics. The e-FOB was successfully applied to detect FQs in complex matrices such as honey and water samples, demonstrating its practical applicability. The ML-3D-QSAR empowered e-FOB offers a revolutionary approach to rapid, on-site screening of antibiotic residues, improving detection efficiency and reducing costs while protecting public health.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117666"},"PeriodicalIF":10.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of fluorescence sensor array for discrimination subtypes of aminoglycosides leveraging MOF-based inhibition reactions and thiol-response metal nanoclusters 利用基于mof的抑制反应和巯基响应金属纳米团簇制备分辨氨基糖苷亚型的荧光传感器阵列

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-03 DOI: 10.1016/j.bios.2025.117652

Jinzhu Ma , Xuezi Lu , Song Liu , Yu-e Shi , Linfeng Cui , Zhe Wang , Henggang Wang , Zhenguang Wang

{"title":"Fabrication of fluorescence sensor array for discrimination subtypes of aminoglycosides leveraging MOF-based inhibition reactions and thiol-response metal nanoclusters","authors":"Jinzhu Ma , Xuezi Lu , Song Liu , Yu-e Shi , Linfeng Cui , Zhe Wang , Henggang Wang , Zhenguang Wang","doi":"10.1016/j.bios.2025.117652","DOIUrl":"10.1016/j.bios.2025.117652","url":null,"abstract":"<div><div>Detection and discrimination aminoglycoside (AG) subtypes are crucial, which remains a formidable challenge. In this study, a sensor array for differentiating of AGs was developed. This was achieved by integrating the inhibitory effect of AGs on the acetylcholinesterase (AChE)-like activities of Al<sup>3+</sup> decorated MOF-808 (MOF-808-Al) and the thiol-response fluorescence of metal nanoclusters (NCs). MOF-808-Al exhibited AChE-like activities, which catalyzed the decomposition of AChE into thiocholine, ascribed to the synergistic effect of metal–OH and Lewis acid sites. Thiocholine quenched the green fluorescence of Au NCs by forming Au-S bonds. In contrast, the blue-emissive Cu NCs showed high resistance to thiocholine. AGs inhibited the AChE-like activities of MOF-808-Al through forming stronger interactions with metal and Lewis acid sites compared to acetylthiocholine. By analyzing the fluorescence changes of green- and blue-emissive metal NCs, a sensor array was constructed, and five subtypes of AGs were quantitatively detected and discriminated, with a limit of detection and limit of quantitation of 11.2 μM and 12.9 μM, respectively. The discrimination of mixed AGs in both buffer solutions and practical samples was also successfully achieved, indicating the great potential for practical applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117652"},"PeriodicalIF":10.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Music-Box: Controlling microfluidic flow with customized melodies for point-of-care testing 音乐盒：控制微流体流动与定制的旋律点护理测试

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-02 DOI: 10.1016/j.bios.2025.117653

Bangfeng Wang , Yangcheng Liu , Shunji Li , Ying Zhang , Dongjuan Chen , Mingxuan Li , Zetai Liu , Peng Chen , Yiwei Li , Xiaojun Feng , Bi-Feng Liu , Wei Lin

{"title":"Music-Box: Controlling microfluidic flow with customized melodies for point-of-care testing","authors":"Bangfeng Wang , Yangcheng Liu , Shunji Li , Ying Zhang , Dongjuan Chen , Mingxuan Li , Zetai Liu , Peng Chen , Yiwei Li , Xiaojun Feng , Bi-Feng Liu , Wei Lin","doi":"10.1016/j.bios.2025.117653","DOIUrl":"10.1016/j.bios.2025.117653","url":null,"abstract":"<div><div>The global prevalence of Human Papillomavirus (HPV) underscores an urgent need for solutions of accessible and rapid diagnostics. Although microfluidic chips have paved a promising pathway for achieving point-of-care-testing (POCT), the reliance on bulky and high-cost pumps has restricted their applicability in decentralized settings. Here, we present \"Music-Box\", a system leveraging a novel actuation mechanism based on the asymmetric motion of the speaker to achieve precise microfluidic control. By eliminating the need for fixed unidirectional structures, this system enables bidirectional fluid manipulation with high precision. Coupled with a smartphone, Music-Box offers a sample-to-result workflow for HPV nucleic acid detection. Clinical verification using 50 hospital-sourced samples reveals that the device has a 97.3 % sensitivity and 100 % specificity for HPV detection. By integrating portability, affordability, and precision, Music-Box redefines the method of POCT, enabling scalable diagnostics not only under resource-limited settings but also adaptable to scenarios of various infectious diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117653"},"PeriodicalIF":10.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanohole-in-microsphere array (NiMA): An ultrasensitive digital-SERS tool for serum diagnosis of rheumatoid arthritis 微球纳米孔阵列（NiMA）：一种用于类风湿性关节炎血清诊断的超灵敏数字sers工具

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-02 DOI: 10.1016/j.bios.2025.117655

Mengyuan Wang , Lulu Cao , Huijuan Zhang , Chuping Zhang , Xiaolin Sun , Yanping Yuan , Xinyang Guo , Xiaoran Guo , Yan Zhao , Yijian Jiang , Zhanguo Li , Yinzhou Yan

{"title":"Nanohole-in-microsphere array (NiMA): An ultrasensitive digital-SERS tool for serum diagnosis of rheumatoid arthritis","authors":"Mengyuan Wang , Lulu Cao , Huijuan Zhang , Chuping Zhang , Xiaolin Sun , Yanping Yuan , Xinyang Guo , Xiaoran Guo , Yan Zhao , Yijian Jiang , Zhanguo Li , Yinzhou Yan","doi":"10.1016/j.bios.2025.117655","DOIUrl":"10.1016/j.bios.2025.117655","url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is a serious chronic systemic autoimmune disease leading to joint destruction for progressive disability. Accurate non-invasive diagnosis of RA in the early stages is vital to timely precision treatment. The advancements of surface-enhanced Raman spectroscopy (SERS) in label-free liquid biopsy for noninvasive diagnosis have been witnessed in the past decade. The recently-developed digital SERS (dSERS) further overcomes the spectral intensity fluctuation, strengthening reliability and robustness for quantitative analysis. Here we propose a high-throughput dSERS substrate by <em>fs</em>-laser parallel fabrication utilizing microsphere-generated photonic nanojet (PNJ), by which the nanohole-in-microsphere array (NiMA) is obtained with the minimum diameter of 115 nm and depth of 600 nm. The Au-nanoparticle-coated NiMA possesses high precision in liquid sampling (10<sup>−18</sup> L) and ultralow limit of detection. The discrete nanohole distribution with high homogeneity guarantees the independent acquisition of Raman spectrum for accuracy of Poisson's statistics in a small spectral dataset volume. The NiMA substrate is employed to collect the sera and diagnose by quantitative recognition of three biomarkers (homocitrulline, L-tryptophan and L-citrulline) in sera by dSERS approach. The accuracy of 88.0 %, sensitivity of 92.9 % and specificity of 81.8 % in distinguishing RA patients manifests the feasibility of serum dSERS protocol for clinical early-stage diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117655"},"PeriodicalIF":10.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CRISPR/Cas12a-powered liquid crystal aptasensor for point-of-care detection of prostate-specific antigen for early-stage cancer diagnosis 一种基于CRISPR/ cas12a的液晶适体传感器，用于前列腺特异性抗原的即时检测，用于早期癌症诊断

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-02 DOI: 10.1016/j.bios.2025.117651

Zahra Khoshbin , Elham Sameiyan , Fatemeh Alizadeh , Mohammad Ramezani , Mona Alibolandi , Khalil Abnous , Seyed Mohammad Taghdisi

{"title":"A CRISPR/Cas12a-powered liquid crystal aptasensor for point-of-care detection of prostate-specific antigen for early-stage cancer diagnosis","authors":"Zahra Khoshbin , Elham Sameiyan , Fatemeh Alizadeh , Mohammad Ramezani , Mona Alibolandi , Khalil Abnous , Seyed Mohammad Taghdisi","doi":"10.1016/j.bios.2025.117651","DOIUrl":"10.1016/j.bios.2025.117651","url":null,"abstract":"<div><div>Herein, an ultrasensitive aptasensor was developed for monitoring prostate-specific antigen (PSA) levels for the first time by incorporating the catalytic activity of CRISPR/Cas12a assembly and directional orientation of liquid crystal (LC) molecules. In the absence of PSA, the specific aptamer was hybridized with crRNA segment of the CRISPR/Cas12a assembly, which activated it for cleaving the ssDNA strand on the sensing substrate. This resulted in a dark background of the sensing substrate. While the assembly was unable to cleave the ssDNA sequence in the presence of PSA, due to forming the PSA-aptamer hybrid and disabling the enzymatic assembly, visualizing the colorful polarized sensing substrate. By changing the polarized background of the LC aptasensor from murky to multicolored, PSA could be detected ranging from 1 ag mL<sup>−1</sup>-1 pg mL<sup>−1</sup> and 1 pg mL<sup>−1</sup>-1 ng mL<sup>−1</sup> with a detection limit of 0.28 ag mL<sup>−1</sup>. With the potency to determine PSA in the spiked human serum samples with the recovery amounts of 97.87–99.57 %, the CRISPR/Cas12a-powered LC aptasensor is a reliable test kit for cancer screening. This was also confirmed by comparing the results obtained from the LC aptasensor with those of ELISA analysis for human serum samples of some patients. Being ultrasensitive, cost-effective, label-free, and user-friendly, the aptasensor can be an efficient future portable gadget for early-stage cancer monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117651"},"PeriodicalIF":10.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing the stability of Hg2+-mediated self-duplexes: Effects of thymine residues position and number via a single-molecule protein nanopore sensing technique 通过单分子蛋白质纳米孔传感技术探测Hg2+介导的自双链的稳定性：胸腺嘧啶残基位置和数量的影响。

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-05-30 DOI: 10.1016/j.bios.2025.117626

Isabela Dragomir, Irina Schiopu, Alina Asandei

{"title":"Probing the stability of Hg2+-mediated self-duplexes: Effects of thymine residues position and number via a single-molecule protein nanopore sensing technique","authors":"Isabela Dragomir, Irina Schiopu, Alina Asandei","doi":"10.1016/j.bios.2025.117626","DOIUrl":"10.1016/j.bios.2025.117626","url":null,"abstract":"<div><div>In this study, we report for the first time that mercury metal ions (Hg<sup>2+</sup>) can stabilize self-duplexes that would otherwise fail to hybridize. We designed two short DNA fragments containing varying numbers of thymine residues, positioned either in the central region or at the terminal ends of the resulting double-stranded structures formed via non-canonical T-Hg<sup>2+</sup>-T base pairs. The stability and structural properties of these newly formed duplexes were investigated using single-molecule nanopore electrophysiology. Our results demonstrated that duplexes with metal-coordinated base pairs located in the central region could be unzipped with relative ease, whereas those with terminal T-Hg<sup>2+</sup>-T pairs exhibited remarkable structural rigidity, resisting modification even under high external force. We emphasize that both the position and the number of thymine residues within the DNA strands are critical key players in the hybridization efficiency and the stability of duplexes. This novel coordination chemistry approach holds significant potential for the development of highly responsive, label-free nanopore sensors for metal ions, the facilitation of strand self-recognition, and the precise detection of thymine positioning within DNA duplexes.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"285 ","pages":"Article 117626"},"PeriodicalIF":10.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0