Biosensors and Bioelectronics最新文献

{"title":"Multimodal activity-affinity assay of ADAM-10 extracellular vesicles in untreated plasma reveals metastatic stage of colorectal cancer","authors":"Tiger Haoran Shi ,&nbsp;John Alex Sinclair ,&nbsp;Youwen Zhang ,&nbsp;Xuemin Lu ,&nbsp;Sonu Kumar ,&nbsp;Xin Lu ,&nbsp;Satyajyoti Senapati ,&nbsp;Hsueh-Chia Chang","doi":"10.1016/j.bios.2026.118517","DOIUrl":"10.1016/j.bios.2026.118517","url":null,"abstract":"<div><div>Metalloproteinases (MPs) such as a-disintegrin and metalloproteinase-10 (ADAM-10) are key drivers of extracellular matrix remodeling during tumor progression, yet MP-based liquid biopsy tests have not reached clinical utility. Here, we show that active ADAM-10 is selectively enriched on the surface of circulating extracellular vesicles (EVs) in the plasma of colorectal cancer patients. Our findings further suggest ADAM-10+ EVs are locally enriched in dense pre-metastatic tumor extracellular matrices and subsequently accumulate in blood post-metastasis. To capture these unique signatures of disease progression, an ADAM-10 activity assay is integrated with a novel size-sensitive Immuno-Janus Particle affinity assay for characterizing ADAM-10+ EVs in untreated plasma. In a 43-patient colorectal cancer cohort, this multimodal platform distinguishes healthy, pre-metastatic, and metastatic states with 95% overall accuracy. When combined with lipidomics as a third modality, the platform correctly determines 97.4% cancer stage accuracy, with only one misclassification. This study establishes a multimodal EV-based activity/affinity assay as a robust framework for liquid biopsy, providing accurate cancer staging, improved prognostics, and offering a potential platform for pan-disease diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"302 ","pages":"Article 118517"},"PeriodicalIF":10.5,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146196854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A wearable system enabling acute stress monitoring and closed-loop mitigation through transcutaneous median nerve stimulation","authors":"Farhan N. Rahman ,&nbsp;Prabhkirat S. Bindra ,&nbsp;Afra Nawar ,&nbsp;H. Trask Crane ,&nbsp;Jesus Antonio Sanchez-Perez ,&nbsp;John A. Berkebile ,&nbsp;Onur Selim Kilic ,&nbsp;Chuoqi Chen ,&nbsp;Vikram Abbaraju ,&nbsp;Christopher J. Nichols ,&nbsp;Jacob M. Cook ,&nbsp;Samer Mabrouk ,&nbsp;Asim H. Gazi ,&nbsp;Jin-Oh Hahn ,&nbsp;Omer T. Inan","doi":"10.1016/j.bios.2026.118461","DOIUrl":"10.1016/j.bios.2026.118461","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Acute stress, in moderation, helps to prepare the body to overcome mental and physical challenges. However, excessive bouts of acute stress can be detrimental to the cardiovascular system and are a risk factor for cardiovascular disease and sudden cardiac death. Transcutaneous median nerve stimulation (tMNS) is a promising therapy for the mitigation of acute stress through peripheral neuromodulation, but the optimal delivery of tMNS for stress mitigation may require continuous monitoring of acute stress events for targeted delivery.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;div&gt;The purpose of this study was to develop a wearable system capable of continuous closed-loop acute stress monitoring and mitigation through non-invasive cardiovascular sensing and tMNS respectively.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;A wearable wrist-worn device capable of sensing three channels of photoplethysmogram (PPG) and tri-axial accelerometry was designed. Pulse rate (PR) and PPG amplitude (PPGamp) were extracted from the acquired green PPG signal, while tMNS was delivered at varying intensities by custom-designed analog circuitry onboard the device. A companion app was used to wirelessly set stimulation levels by communicating with the device's microcontroller using Bluetooth low energy. The device was validated against bench-top sensors in a study with 19 healthy participants involving acute mental and physical stressors as well as tMNS. Repeated-measures correlation and Bland-Altman analyses were performed to compare PR extracted from 9904 5-s windows of PPG from the device and heart rate (HR) extracted beat-by-beat from bench-top electrocardiogram (ECG) and averaged across the same windows. Statistical tests were also performed to analyze differences in mean PR and PPGamp from baseline metrics across the acute stress and tMNS protocol.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;PR extracted from our device correlated (r = 0.871, p &lt; 0.001) and agreed (mean difference: 0.51 bpm, 95 % limits of agreement: 6.58 bpm, 5.57 bpm) strongly with HR extracted from bench-top ECG. We found decreases in mean PPGamp from baseline during stressors, while application of tMNS alongside stressors increased PPGamp back to baseline levels, and continued delivery of tMNS post-stressor further increased PPGamp to a significant difference from baseline. Significant reductions in PR as compared to baseline post-physical stressor also mirrored these findings, suggesting that our wearable device can track elevations in acute stress through cardiovascular monitoring while also mitigating the effects of acute stress through tMNS.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;Our device is the first wearable, to our knowledge, to enable continuous monitoring of acute stress through cardiovascular sensing and feature extraction while mitigating acute stress through peripheral neuromodulation. Future work should test the device in ambulatory settings and investigate potential ","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118461"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146147297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-mode electrochemiluminescence/SERS biosensor for B-type natriuretic peptide based on a T7–CRISPR/Cas13a cascade and a CsPbBr3@PDA@Au perovskite interface","authors":"Yang Li ,&nbsp;Haozhen Ren ,&nbsp;Runze Wu ,&nbsp;Jiahui Wang ,&nbsp;Yuanxun Gong ,&nbsp;Qianli Tang ,&nbsp;Xianjiu Liao ,&nbsp;Kai Zhang ,&nbsp;Longjian Huang ,&nbsp;Jihua Wei","doi":"10.1016/j.bios.2026.118484","DOIUrl":"10.1016/j.bios.2026.118484","url":null,"abstract":"<div><div>B-type natriuretic peptide (BNP) is an important biomarker for cardiovascular diseases, motivating sensitive and robust quantification in complex matrices. Here we report a single-electrode, anti-correlated dual-mode electrochemiluminescence/surface-enhanced Raman scattering (ECL/SERS) biosensor that couples split-aptamer recognition with a solution-phase T7 transcription–CRISPR/Cas13a cascade and an interfacial toehold-mediated strand-displacement “probe-stripping” transduction on a CsPbBr₃@PDA@Au-modified glassy carbon electrode. BNP binding releases an active T7 template to generate trigger RNA, which activates Cas13a collateral cleavage to produce initiator strands for interfacial unlocking. The interface reaction removes ferrocene/Raman co-labeled probes, synchronizing ECL turn-on with SERS turn-off and enabling ratiometric quantification (R = I_ECL/I_SERS) to suppress common-mode variability. The sensor provides BNP determination over 0–10⁶ aM with log-linear single-mode calibrations and a continuous ratiometric response. Selectivity was validated against multiple interferents and the structurally related peptide NT-proBNP at the same concentration (10⁶ aM), showing negligible ratiometric change relative to BNP. Serum-sample evaluation and stability tests further support feasibility in complex matrices. This work establishes a cascade-to-interface ratiometric strategy for robust protein biosensing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118484"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146147347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically encoded biosensor of apoptosis and necrosis reveals the dynamics and biphasic cytotoxicity by Anti-EGFR CAR-T cells","authors":"Aijaz Ahmad Rather ,&nbsp;Aman Munirpasha Halikar ,&nbsp;Ritika Sachdeva ,&nbsp;Shivanshu Kumar Tiwari ,&nbsp;T.R. Santhoshkumar","doi":"10.1016/j.bios.2026.118471","DOIUrl":"10.1016/j.bios.2026.118471","url":null,"abstract":"<div><div>Precise and quantitative evaluation of CAR-T cell-induced cytotoxicity by resolving distinct cell death phenotypes in live cells remains a critical unmet need in the development of immunotherapies. Discriminating between apoptosis and necrosis induced by CAR-T therapy at single-cell resolution is essential for elucidating cytotoxic mechanisms and predicting therapeutic outcomes. Yet, most live cell assays lack the spatiotemporal sensitivity to do so. Here, we present a genetically encoded dual-biosensor system that concurrently monitors caspase-3/7 activation and mitochondrial integrity, enabling the real-time distinction between apoptosis and necrosis. The platform combines a FRET-based caspase sensor (RealCas3) and a mitochondria-targeted DsRed reporter (MitoDsRed) that exhibits compatibility with both microscopy imaging and flow cytometry. We applied this platform to functionally profile anti-EGFR CAR-T cells and uncover a biphasic cytotoxicity pattern, marked by early caspase-3/7 induced apoptosis, followed by a delayed necrosis. Notably, the system enables the time-resolved detection of secondary necrosis, a delayed immunogenic death phenotype that is often misclassified in conventional assays. The developed platform offered a robust and scalable approach for real-time functional phenotyping of immune effector responses, addressing a key gap in evaluating cell death mechanisms during immunotherapy development. By enabling the time-resolved quantification of tumor cell demise along with the mode of death, this dual-sensor system established a mechanistic framework for evaluation and rational design of safer and more effective next-generation immunotherapies.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118471"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene hybrid nanoprobes for targeted microbial sensing and ultralow-energy, deep-tissue, noninvasive multiphoton imaging in the NIR-I/II region","authors":"Wen-Shuo Kuo ,&nbsp;Yen-Sung Lin ,&nbsp;Chia-Yuan Chang ,&nbsp;Jiu-Yao Wang ,&nbsp;Pei-Chi Chen ,&nbsp;Shih-Wen Tseng ,&nbsp;Chiao-Yun Lin ,&nbsp;Chan-Chi Chang ,&nbsp;Shang-Rung Wu","doi":"10.1016/j.bios.2026.118506","DOIUrl":"10.1016/j.bios.2026.118506","url":null,"abstract":"<div><div>Nitrogen (N) doping and amino functionalization markedly enhance the electron-donating capacity of graphene quantum dots (GQDs), thereby improving charge-transfer efficiency in amino-N-GQDs and yielding substantially superior photophysical performance compared with amino-free N-GQDs and N-free amino-GQDs. Further optimization was achieved through conjugation of amino-N-GQDs with sulfur- and nitrogen-rich polymers, polystyrene sulfonate and polyethylenimine, resulting in amino-N-GQD-polymer nanohybrids with significantly improved optical behavior. These hybrid nanostructures exhibited high quantum yields, excellent photostability, negligible reactive oxygen species generation, and strong two-photon luminescence, positioning them as promising contrast agents for nonlinear bioimaging. To enable molecular specificity, antibody functionalization was incorporated. When conjugated with anti-lipopolysaccharide or anti-TasA antibodies, the nanohybrids selectively targeted <em>Escherichia coli</em> (<em>E. coli</em>) and <em>Bacillus subtilis</em> (<em>B. subtilis</em>), generating bright fluorescence, strong signal intensity, and high signal-to-noise ratios under two-photon excitation. Using a custom-built Ti:sapphire laser system operating at 970 nm (near-infrared-II region), imaging depths of up to 270 μm were achieved with ultralow excitation energies, 42.96 nJ pixel⁻¹ for <em>E. coli</em> and 35.14 nJ pixel⁻¹ for <em>B. subtilis</em>, acquired over 100 scans (total exposure = 0.666 s). The nanohybrids produced two-photon luminescence using only 1/49 and 1/36 of the energy required for cellular autofluorescence, corresponding to ∼2401- and ∼1296-fold signal enhancements, respectively. This remarkable efficiency supports deep, noninvasive imaging and underscores the potential of amino-N-GQD-polymer nanohybrids as versatile near-infrared-I/II-responsive probes for next-generation biomedical imaging applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118506"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scalable fabrication of conductive silk textiles for integrated motion monitoring and adaptive thermal management","authors":"Wen-Wu Zhang,&nbsp;Ting Liu,&nbsp;Shou-Shan Yu,&nbsp;Ke-Zheng Chen,&nbsp;Sheng-Lin Qiao","doi":"10.1016/j.bios.2026.118480","DOIUrl":"10.1016/j.bios.2026.118480","url":null,"abstract":"<div><div>The development of multifunctional smart textiles with integrated motion sensing and thermal management is critical for advanced wearable electronics, especially in health monitoring, injury prevention, and environmental adaptation. Herein, we develop a durable, scalable MXene-functionalized conductive silk textile (HMS-ST) with combined negative gauge factor strain sensing and Joule heating properties. HMS-ST was prepared by impregnating silk yarns with sericin (SC)-modified MXene (MS) ink, followed by hexamethylene diisocyanate (HDI) cross-linking to enhance mechanical robustness, wash durability, and oxidative stability. The textile shows an ultrawide strain detection range (1-100%), high negative gauge factor (GF = −6.08), and minimal hysteresis for high-fidelity motion monitoring. Additionally, it delivers efficient Joule heating, reaching ∼40 °C stably at 9 V with negligible thermal fluctuation under 50% strain. This work presents a mechanically resilient, flexible, scalable electronic textile platform for real-time motion tracking and adaptive thermal regulation, with great potential in biomechanical analysis, rehabilitation engineering, and extreme environmental protection.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118480"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146147299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-responsive nanozyme-mediated enrichment-confinement dual strategy breaks the non-responsive barrier for Triphenols: Highly responsive detection and differentiation of tea","authors":"Jie Li ,&nbsp;Qing Han ,&nbsp;Xingna Zheng ,&nbsp;Linxue Zhen ,&nbsp;Yongxin Li ,&nbsp;Hui Huang","doi":"10.1016/j.bios.2026.118503","DOIUrl":"10.1016/j.bios.2026.118503","url":null,"abstract":"<div><div>The predominant ortho-trihydroxyphenyl structure in tea polyphenols requires highly specific sensing materials for their detection. However, both natural enzymes and current polyphenol oxidase-like nanozymes exhibit inadequate responsiveness to this specific configuration. To overcome this limitation, a dual \"enrichment-confinement\" strategy was proposed, effectively addressing the suboptimal detection performance for triphenolic compounds. The efficacy of this strategy relies on a switchable hydrophilic-to-hydrophobic transition: substrate enrichment is driven by extended hydrophilic chains at low temperatures, whereas heating induces hydrophobic collapse and nanoscale contraction, thereby activating spatial confinement. This conformational change not only shortens the interactive distance between the nanozyme and the substrate but also mitigates undesirable substrate aggregation. Theoretical calculations further reveal that the interactions between the nanozyme and the thermosensitive polymer are primarily governed by van der Waals forces and hydrogen bonding, which notably preserve the nanozyme intrinsic catalytic activity. This method enables the precise differentiation of tea polyphenols with a detection limit as low as 100 nM. Notably, this technology extends beyond the detection of tea triphenols, broadening the application scope of polyphenol oxidase-like nanozymes and providing a novel paradigm for the sensitive detection of diverse triphenolic compounds.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118503"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146147348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finger pulp-inspired flexible pressure sensor array for gap balance monitoring during total knee arthroplasty","authors":"Ziheng Wang ,&nbsp;Tianci Zhang ,&nbsp;Hongyi Shao ,&nbsp;Yixin Zhou ,&nbsp;Yihao Chen ,&nbsp;Xue Feng","doi":"10.1016/j.bios.2026.118504","DOIUrl":"10.1016/j.bios.2026.118504","url":null,"abstract":"<div><div>Total knee arthroplasty (TKA) is one of the most effective treatments for end-stage knee osteoarthritis (∼1 million annual surgeries globally), where the biomechanical stability of the implanted prosthesis is crucial for lower limb mechanical axis alignment and postoperative normal gait. Most surgical failures and revisions are caused by intraoperative soft tissue imbalance. It heavily relies on surgeons' experience. Here, we present a wireless digital system featuring an ultrathin finger pulp-inspired flexible pressure sensor array, enabling balance monitoring in the clearance between the tibial component and polyethylene spacer, providing a specification-independent option. It addresses the inherent trade-off between body mass-level measurement range and minute force change detection during balancing. The bio-inspired sensor features fingerprint/mechanoreceptor-shaped trans-scale microstructures, achieving high linear sensitivity (0.016 N^-1/0.002 N^-1), wide range (0-150 N per sensor and &gt;440 N total), and fast response/recovery time (50/75 ms), with stability (&gt;1000 cycles). Prosthetic bone validation demonstrates bilateral real-time quantitative force visualization across flexion angles (0°, 45°, 90°). Cadaveric validation with authentic bone mass and intraoperative force variations confirms clinical effectiveness and compatibility.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118504"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triple-signal lateral flow immunoassay for the detection of bovine casein using an M13 phage-displayed nanobody and MnO2 nanozyme conjugate","authors":"Wenyi Dong ,&nbsp;Ajuan You ,&nbsp;Yulou Qiu ,&nbsp;Wanwan Liu ,&nbsp;Jia Qiu ,&nbsp;Yutao Zhu ,&nbsp;Biao Zhang ,&nbsp;Xianshu Fu ,&nbsp;Zihong Ye ,&nbsp;Xiaoping Yu","doi":"10.1016/j.bios.2026.118486","DOIUrl":"10.1016/j.bios.2026.118486","url":null,"abstract":"<div><div>Cow's milk allergy is a significant global health concern, with bovine casein recognized as its predominant allergen. Rapid, sensitive, and accurate detection of bovine casein in food products is therefore essential. Here, a triple-signal lateral flow immunoassay (LFIA) for casein detection was developed by integrating an M13 phage-displayed nanobody (M13-Nb) with multifunctional MnO₂ nanosheets (MnO₂ NSs) as the signal probe. Anti-casein M13-Nb was selected from a naïve phage-displayed nanobody library through four rounds of biopanning. MnO₂ NSs exhibiting intrinsic colorimetric properties, oxidase-like catalytic activity, and photothermal capability were synthesized and conjugated with M13-Nb, enabling the MnO₂ NSs@M13-Nb-based LFIA to generate colorimetric, amplified colorimetric, and photothermal signals simultaneously. The visual limits of detection (vLOD) for these modes were 125, 15.63, and 31.25 ng/mL, respectively, with corresponding quantitative LODs of 60.97, 6.72, and 23.88 ng/mL. The assay showed high specificity and accuracy, with recovery rates of 87.92%–114.25% in spiked food samples. This triple-signal LFIA provides a rapid and reliable platform for casein detection in foods and highlights the potential of combining M13-Nb and MnO₂ NSs for on-site monitoring of food allergens.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118486"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Affordable plasmonic biosensing: democratizing SERS with scalable, field-compatible substrate fabrication","authors":"Saransh Arora ,&nbsp;Vighnesh Ginde ,&nbsp;Swati Tanwar ,&nbsp;Marwan El Chazli ,&nbsp;Raj Bhatt ,&nbsp;Evan Edelman ,&nbsp;Debadrita Paria ,&nbsp;Ishan Barman","doi":"10.1016/j.bios.2026.118487","DOIUrl":"10.1016/j.bios.2026.118487","url":null,"abstract":"<div><div>Efficient and accurate plasmonic biosensing in the field remains a challenge. Despite its potential to revolutionize point-of-care (PoC) diagnostics through unparalleled sensitivity and precise molecular fingerprinting, adoption of traditional surface-enhanced Raman spectroscopy (SERS) has not proven feasible in the field. High production costs, complex fabrication, specialized equipment, and persistent issues with stability and reproducibility impede development for PoC use. Addressing these challenges, this study innovates a democratized and cost-effective fabrication kit that enables the SERS substrate production using commonly available materials and straightforward electrochemistry without compromising on sensitivity and reproducibility. Importantly, this method leverages commercially available bottled water and simple battery-powered fabrication, eliminating reliance on a power grid and enabling local production of biosensors in resource-restricted regions. The cost of producing the fabrication kit is $39.54 with raw materials purchased at bulk retail prices, while the cost of consumables for fabricating each test is just 1.33¢. We demonstrate the efficacy of these substrates in two critical applications: the rapid detection of bacteria and pesticides. We detect trace levels of pesticides such as Thiram and Thiabendazole down to 0.1 ppm using a digital SERS approach. We also demonstrated the identification of bacteria isolated from culture, namely <em>Escherichia coli</em> and <em>Bacillus subtilis.</em> We envision that this label-free, high-sensitivity substrate, when paired with portable Raman spectrometers, could open doors for a new era of field-deployable biosensing, paving the way for its adoption for a plethora of applications, from public health to food testing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118487"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}