Biosensors and Bioelectronics最新文献

Cell elasticity measurement and sorting based on microfluidic techniques: Advances and applications.

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-03-01 Epub Date: 2024-11-25 DOI: 10.1016/j.bios.2024.116985

Jiahuan Yang, Yong Liu, Bin Li, Jingjing Li, Sheng Yan, Huaying Chen

{"title":"Cell elasticity measurement and sorting based on microfluidic techniques: Advances and applications.","authors":"Jiahuan Yang, Yong Liu, Bin Li, Jingjing Li, Sheng Yan, Huaying Chen","doi":"10.1016/j.bios.2024.116985","DOIUrl":"10.1016/j.bios.2024.116985","url":null,"abstract":"Cell elasticity serves as a crucial physical biomarker that reflects changes in cellular structures and physiological states, providing key insights into cell behaviors. It links mechanical properties to biological function, highlighting its importance for understanding cell health and advancing biomedical research. Microfluidic technologies, with their capabilities for precise manipulation and high-throughput analysis, have significantly advanced the measurement of cell elasticity and elasticity-based cell sorting. This paper presents a comprehensive overview of advanced microsystems for assessing cell elasticity, discussing their advantages and limitations. The biomedical applications of elasticity-based sorting are highlighted, including cell classification, clinical diagnosis, drug screening, and stem cell differentiation prediction. The paper addresses the current challenges in the field, such as limited measurement efficiency and scalability, and explores future research directions, including the development of automated, high-throughput systems and the integration of elasticity measurements into practical biomedical applications. These advancements aim to deepen our understanding of cellular mechanics, improve diagnostic precision, and foster the development of novel therapeutic strategies. Ultimately, this work emphasizes the potential of cell elasticity as a key parameter in advancing disease diagnosis and therapeutic research.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"116985"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical magneto-immunoassay based on Mycobacterium leprae mimotope for serological diagnosis of leprosy.

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-03-01 Epub Date: 2024-12-03 DOI: 10.1016/j.bios.2024.117022

Sthéfane Valle de Almeida, Pablo Roberto Lins da Silva, Wilson Tiago Fonseca, Tássia Regina de Oliveira, Cristiane Zocatelli Ribeiro, Juliana de Moura, Ronaldo Censi Faria

{"title":"Electrochemical magneto-immunoassay based on Mycobacterium leprae mimotope for serological diagnosis of leprosy.","authors":"Sthéfane Valle de Almeida, Pablo Roberto Lins da Silva, Wilson Tiago Fonseca, Tássia Regina de Oliveira, Cristiane Zocatelli Ribeiro, Juliana de Moura, Ronaldo Censi Faria","doi":"10.1016/j.bios.2024.117022","DOIUrl":"10.1016/j.bios.2024.117022","url":null,"abstract":"Leprosy is an infectious disease classified as Neglected Tropical Disease (NTD) by the World Health Organization (WHO). Its diagnosis is challenging, relying on clinical symptoms and invasive procedures. Delays can cause severe physical disability, including hand, foot, and eye impairments. Herein, we describe the development of a magneto-immunoassay based on an electrochemical disposable microfluidic device for the serological diagnosis of leprosy. The assay makes use of magnetic particles (MPs) modified with Mycobacterium leprae mimotope as a probe to capture and separate target-specific antibodies from serum samples. The antibody anti-M. leprae was detected using the anti-human antibody IgG conjugated with horseradish peroxidase enzyme as an electrochemical maker. The magneto-immunoassay was applied in serum samples from healthy subjects and patients diagnosed with leprosy classified as paucibacillary (PB) and multibacillary (MB). The results showed that the method could differentiate between negative and positive patients (AUC = 0.990) with high clinical sensitivity (91.2%) and specificity (100.0%). In addition, the assay showed the capability to differentiate between paucibacillary and multibacillary with sensitivities of 88.9% and 93.7%, respectively, indicating that the device can be used to guide the treatment of positive cases. Thus, magneto-immunoassay based on the use of a disposable microfluidic device demonstrated to be a valuable tool for the early diagnosis of leprosy which can help the public health system to reduce disability cases and even control and eliminate the disease by interrupting the chain of transmission.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117022"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digital microfluidics with integrated Raman sensor for high-sensitivity in-situ bioanalysis.

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-03-01 Epub Date: 2024-12-04 DOI: 10.1016/j.bios.2024.117036

Wenbo Dong, Rongxin Fu, Nan Zhang, Jing Zhao, Yudan Ma, Han Cui, Jiangjiang Zhang, Zipeng Zhao, Hang Li, Yunxia Zhao, Yao Lu, Zhizhong Chen, Tianming Xu, Huikai Xie, Qian Yu, Shuailong Zhang

{"title":"Digital microfluidics with integrated Raman sensor for high-sensitivity in-situ bioanalysis.","authors":"Wenbo Dong, Rongxin Fu, Nan Zhang, Jing Zhao, Yudan Ma, Han Cui, Jiangjiang Zhang, Zipeng Zhao, Hang Li, Yunxia Zhao, Yao Lu, Zhizhong Chen, Tianming Xu, Huikai Xie, Qian Yu, Shuailong Zhang","doi":"10.1016/j.bios.2024.117036","DOIUrl":"10.1016/j.bios.2024.117036","url":null,"abstract":"This study introduces an advanced bioanalytical platform that combines digital microfluidics (DMF) with Raman spectroscopy, effectively addressing common issues in bioanalysis such as sample contamination, excessive consumption of samples and reagents, and manual handling. Our innovative system is engineered to handle diverse sample types and enables both sample preparation and in-situ analysis on a single device, utilizing less than 5 μL of samples and reagents. It incorporates a Translucent Raman Enhancement Stack (TRES) sensor, which boosts the detection signal, and includes droplet-driving functionality for automated processing of complex samples in a compact setting. The hydrophilic surface of the TRES sensor draws analytes into the detection zone, expediting sample attachment and enhancing the surface-enhanced Raman scattering (SERS). Moreover, the TRES sensors are cost-effective, straightforward to manufacture, and scalable, making them suitable for widespread production and single-use bioanalytical applications. This integrated and automated approach streamlines the sample preparation and analysis processes, enhances detection efficiency, and achieves high sensitivity, excellent linearity, and effective detection of different biochemical analytes. We validate the system's accuracy through on-device enrichment and analysis of exosomes from serum, demonstrating its capability for real-time, on-site analysis of complex biological samples.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117036"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic and quantitative assessment of quercetin for cardiac oxidative stress injury prevention using sensitive cardiomyocyte based biosensing.

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-03-01 Epub Date: 2024-12-06 DOI: 10.1016/j.bios.2024.117045

Jie Chen, Xuelian Lyu, Qunchen Yuan, Chunlian Qin, Han Yu, Dongxin Xu, Jilin Zheng, Hongchun Li, Jiaru Fang, Ning Hu, Yuqun Cai

{"title":"Dynamic and quantitative assessment of quercetin for cardiac oxidative stress injury prevention using sensitive cardiomyocyte based biosensing.","authors":"Jie Chen, Xuelian Lyu, Qunchen Yuan, Chunlian Qin, Han Yu, Dongxin Xu, Jilin Zheng, Hongchun Li, Jiaru Fang, Ning Hu, Yuqun Cai","doi":"10.1016/j.bios.2024.117045","DOIUrl":"10.1016/j.bios.2024.117045","url":null,"abstract":"Myocardial infarction is a leading cause of morbidity and mortality associated with cardiovascular diseases worldwide. Although novel medications and treatments greatly alleviate patient suffering, challenges related to prognostic limit the recovery of cardiac function. Currently, treatment with monomeric compounds displays promise in prognostic interventions for cardiac diseases. However, there is a lack of dynamic and quantitative assessment of cardiomyocyte response to these drugs. Herein, an integrated biosensing platform with a microelectrode array was constructed for label-free, non-invasive, long-term, and real-time recording of cardiomyocyte electrophysiological signals. By analyzing the signals of cardiomyocytes before and after treatment, we established the safe concentration of quercetin in cardiomyocytes and identified its long-term cardiotoxicity. Moreover, quercetin also demonstrated significant protective effects on cardiomyocytes in a H2O2-induced oxidative stress injury model. This study provides a trustworthy platform to evaluate the effects of monomeric compounds on cardiomyocytes, and offers a novel approach for drug screening and efficacy testing in cardiovascular diseases.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117045"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-metal MOF-on-MOF metal-organic gel-based visual sensing platform for ultrasensitive detection of chlortetracycline and D-penicillamine.

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-03-01 Epub Date: 2024-12-05 DOI: 10.1016/j.bios.2024.117012

Zongwu Meng, Tao Zhang, Zerun Zhang, Xiaolong Qu, Bin Wang, Yuzhe Qiu, Pian Wu, Ping Ding

{"title":"Multi-metal MOF-on-MOF metal-organic gel-based visual sensing platform for ultrasensitive detection of chlortetracycline and D-penicillamine.","authors":"Zongwu Meng, Tao Zhang, Zerun Zhang, Xiaolong Qu, Bin Wang, Yuzhe Qiu, Pian Wu, Ping Ding","doi":"10.1016/j.bios.2024.117012","DOIUrl":"10.1016/j.bios.2024.117012","url":null,"abstract":"The widespread presence of antibiotic residues in the environment and food represents a significant threat to human health. In this study, a novel MOF-on-MOF (FCZE) gel with excellent peroxidase activity and strong fluorescence properties successfully synthesized by using Polyvinyl pyrrolidone (PVP) as a cross-linker and H₄BTEC as a ligand. Based on the energy transfer from the FCZE ligand to Eu luminescence, an ultrasensitive fluorescence method was developed for the detection of chlortetracycline (CTC), achieving a limit of detection (LOD) as low as 0.43 nM. Furthermore, the combination of FCZE with the 3,3',5,5'-tetramethylbenzidine (TMB)-hydrogen peroxide (H2O2) system enabled sensitive colorimetric detection of D-penicillamine (D-PA) with an LOD of 8.66 nM. Theoretical calculations reveal that the fluorescence quenching of FCZE by CTC is attributed to the inner filter effect of CTC and its suppression of the H4BTEC excited-state return to the ground state. Furthermore, Fukui function was employed for the first time to clarify the role of D-PA in scavenging free radicals and reducing oxidized TMB (oxTMB), providing a comprehensive understanding of the colorimetric detection mechanism. Additionally, a smartphone-assisted visual detection platform based on FCZE was developed for the detection of CTC and D-PA, with LODs of 0.71 μM and 0.13 μM, respectively. This study highlights the potential of nanomaterials for the simultaneous detection of multiple antibiotics, offering a novel strategy for the rapid and efficient monitoring of antibiotic residues in environmental samples.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117012"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SiO₂/Si interferometers designed for on-site botulinum neurotoxin serotypes B and C quantification and biological activity assessment.

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-03-01 Epub Date: 2024-12-03 DOI: 10.1016/j.bios.2024.117027

D Nanda Kumar, Zina Baider, Shlomo E Blum, Giorgi Shtenberg

{"title":"SiO2/Si interferometers designed for on-site botulinum neurotoxin serotypes B and C quantification and biological activity assessment.","authors":"D Nanda Kumar, Zina Baider, Shlomo E Blum, Giorgi Shtenberg","doi":"10.1016/j.bios.2024.117027","DOIUrl":"10.1016/j.bios.2024.117027","url":null,"abstract":"Botulinum neurotoxins (BoNT), the agent causing botulism, exhibit the highest potency among bacterial toxins and pose a significant threat to both humans and animals. The current in vivo method (mouse lethality assay, MLA) is inappropriate for real-time and pen-side assessment of the occurring outbreak or case. Herein, we describe a reflective-based biosensor capable of detecting the toxin's type and activity state by competitive immunoassay and endopeptidase activity, respectively. Two serotypes (BoNT-B and BoNT-C) were assessed independently by SiO2/Si interferometers using simulating antigens or specific peptides (i.e., synaptosomal-associated protein and vesicle-associated membrane protein). The reflective responses of both platforms were biochemically amplified using 4-chloro-1-naphthol oxidation into insoluble products. The resulting refractive index variations were observed in real-time using reﬂective interferometric Fourier transform spectroscopy. The portable bioassay depicted high sensitivity, selectivity, and near real-time rapid assessment (0.14 and 0.47 pg mL-1 for BoNT-C and BoNT-B, negligible interferences with interfering serotypes, respectively). Additionally, the applicability of the bioassay for monitoring extracted toxins was tested against the in vivo method with satisfactory performance values. Overall, the presented approach can improve field assessment of new botulism incidences by acquiring epidemiological data in near real-time, thus eliminating the need for the MLA approach.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117027"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-resonance optical fiber lossy mode resonance immunoprobe for serum PSA detection.

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-03-01 Epub Date: 2024-12-08 DOI: 10.1016/j.bios.2024.117049

Xiaoshuang Dai, Shuang Wang, Yong Wang, Xu Wang, Xupeng Wu, Xiang Liu, Junfeng Jiang, Tianhua Xu, Tiegen Liu

{"title":"Dual-resonance optical fiber lossy mode resonance immunoprobe for serum PSA detection.","authors":"Xiaoshuang Dai, Shuang Wang, Yong Wang, Xu Wang, Xupeng Wu, Xiang Liu, Junfeng Jiang, Tianhua Xu, Tiegen Liu","doi":"10.1016/j.bios.2024.117049","DOIUrl":"10.1016/j.bios.2024.117049","url":null,"abstract":"Biomarker detection has emerged as an essential complementary approach for early-stage screening of tumors. Conventional methods are constrained by bulky systems, cumbersome operation steps, and low detection accuracy. Here, we demonstrate a dual-resonance optimally configured lossy mode resonance (LMR) immunoprobe for detecting prostate-specific antigen (PSA), a biomarker for prostate cancer (PCa). The dual-resonance structure used in this study introduces low-refractive-index magnesium fluoride (MgF2) as a matching layer before adding indium tin oxide (ITO) as a lossy mode excitation layer to enhance the phase matching between the evanescent wave and the lossy mode to realize dual-resonance. Compared with classical optical fiber LMR, the dual-resonance effect not only improves the detection precision but also features a high accuracy of joint detection. The limit of detection (LOD) of the optical fiber immunoprobe based on dual-resonance for PSA immunoassay can reach down to 52 pg/mL, making it ideally suited for early risk management and prognostic diagnosis of PCa. In addition, the optical fiber immunoprobe is used to detect suspected PCa patients, with a successful recognition rate of 90% compared with that of standard commercially available chemiluminescent assays. This work may pave the way for promising applications of optical fiber immunoprobe for high-precision, high-accuracy, and portable early-stage screening of tumors.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117049"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green fluorescent FM dyes with prolonged retention for 4D tracking of plasma membrane dynamics in live plants under environmental stress.

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-03-01 Epub Date: 2024-12-05 DOI: 10.1016/j.bios.2024.117039

Penglei Wu, Jiaqi Zuo, Zhengdong Han, Xin Peng, Ziqing He, Wenjing Yin, Hui Feng, Engao Zhu, Yuchun Rao, Zhaosheng Qian

{"title":"Green fluorescent FM dyes with prolonged retention for 4D tracking of plasma membrane dynamics in live plants under environmental stress.","authors":"Penglei Wu, Jiaqi Zuo, Zhengdong Han, Xin Peng, Ziqing He, Wenjing Yin, Hui Feng, Engao Zhu, Yuchun Rao, Zhaosheng Qian","doi":"10.1016/j.bios.2024.117039","DOIUrl":"10.1016/j.bios.2024.117039","url":null,"abstract":"Macroscopic phenotypic changes in plants are frequently employed as a means of evaluating the biological response of plants to external environmental stresses. However, the lack of effective observational tools at the microscopic cellular level hinders the ability to fully comprehend the intricacies of this response. Herein, we developed a plasma membrane fluorescent dye with target-activated green emission complemented with conventional FM dyes, and established a four-dimensional (4D) imaging approach based on this dye for spatio-temporal monitoring of plasma membrane dynamics during cellular responses to external environmental stress. A green fluorescent dye, designated FMG-DBO, was constructed by modifying the bridged unit between the aniline donor and the pyridinium acceptor. Its green emission can be combined with that of conventional FM dyes, enabling high-resolution imaging of plant leaf cells containing chlorophyll. The anchoring ability of the dyes was enhanced by incorporating a rigid diaza[2.2.2]octane unit as an anti-permeability group. The long retention time of the FMG-DBO dye in the plasma membrane enables the tracking of three-dimensional dynamics of the plasma membrane of plant cells. Consequently, an FMG-DBO-based four-dimensional imaging approach was established to monitor dynamic changes of plant cells under external environmental stress at the cellular level. The biological responses of two different drought-tolerant rice root cells to drought stress were examined by this four-dimensional imaging approach. It was observed that the two types of rice root cells exhibited disparate responses to the drought environmen. This approach offers alternative cell-level visualization tools for evaluating the biological responses of plant cells under environmental stress.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117039"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

All-in-one microfluidic immunosensing device for rapid and end-to-end determination of salivary biomarkers of cardiovascular diseases.

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-03-01 Epub Date: 2024-12-17 DOI: 10.1016/j.bios.2024.117077

Baihui Liang, Shan Wang, Junmei Zheng, Bin Li, Ningtao Cheng, Ning Gan

{"title":"All-in-one microfluidic immunosensing device for rapid and end-to-end determination of salivary biomarkers of cardiovascular diseases.","authors":"Baihui Liang, Shan Wang, Junmei Zheng, Bin Li, Ningtao Cheng, Ning Gan","doi":"10.1016/j.bios.2024.117077","DOIUrl":"10.1016/j.bios.2024.117077","url":null,"abstract":"Routine screening for cardiovascular diseases (CVDs) through point-of-care assays for at-home or community-based testing of salivary biomarkers can significantly improve patient outcomes. However, its translatability has been hindered by a dearth of biosensing devices that streamline assay procedures for rapid biomarker quantitation. To address this challenge through end-to-end engineering, we developed an in-house, all-in-one microfluidic immunosensing device that integrates on-chip vibration-enhanced incubation, magnetic-assisted separation using immune magnetic bead probes, and colorimetric readout via absorbance measurements. This device enables probe preparation and one-pot immunoassay procedures on a reusable microfluidic chip. By engaging the vibrator with the reaction chamber, the vibration-enhanced incubation module significantly accelerates immune complex formation, drastically reducing the sample-to-answer timeline of approximately 1 h required for room temperature enzyme-linked immunosorbent assay (ELISA) to just under 15 min. We showcase the utility of the device with an on-demand assay for a biomarker panel comprising C-reactive protein (CRP), interleukin 6 (IL-6), and procalcitonin (PCT). The device achieved a linear detection range of 1.75-28 ng mL-1 for CRP and 1.56-100 ng mL-1 for IL-6 and PCT with an R2 > 0.98 for all three biomarkers. The limits of detection were 0.295, 0.400, and 0.947 ng mL-1, respectively. Results from real saliva samples were consistent with standard ELISA (R2 = 0.952). This fully integrated, modular immunosensing device opens up opportunities for household CVD screening and could be adapted for rapid, affordable multiplexed biosensing for other major chronic diseases at the point of care.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117077"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a dual-mode lateral flow assay based on structure-guided aptamers for the detection of capsaicin in gutter oils.

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-03-01 Epub Date: 2024-12-25 DOI: 10.1016/j.bios.2024.117100

Mingwei Qin, Ning Ding, Pengfei Ma, Hongtao Jiang, Yu Li, Peifang Chen, Zhouping Wang, Junsong Yang

{"title":"Development of a dual-mode lateral flow assay based on structure-guided aptamers for the detection of capsaicin in gutter oils.","authors":"Mingwei Qin, Ning Ding, Pengfei Ma, Hongtao Jiang, Yu Li, Peifang Chen, Zhouping Wang, Junsong Yang","doi":"10.1016/j.bios.2024.117100","DOIUrl":"10.1016/j.bios.2024.117100","url":null,"abstract":"The construction of structure-guided aptamers and the ultra-sensitive aptamer-based lateral flow assays (Apt-LFA) integrated detection method hold significant potential for food analysis. Using an engineered modified sequence strategy, we successfully developed the aptamer Cap-1-2M4, significantly enhancing its affinity for capsaicin (CAP) to 0.6197 ± 0.0689 nM. We developed a highly sensitive method for CAP detection based on Vap@AuNFs, leveraging their superior colorimetric and photothermal signals and high aptamer coupling performance. The Vap@AuNFs-based Apt-LFA (Vap@AuNFs-Apt-LFA) achieve signal amplification through catalytic hairpin assembly (CHA) and exonuclease III (Exo III). Additionally, dual-mode (colorimetric and photothermal) quantitative detection via lateral flow test strips was achieved by capturing the nucleic acid effect of Vap@AuNFs. The limit of detection (LOD) of Vap@AuNFs-Apt-LFA by colorimetric/photothermal method was 0.043 ng mL-1 and 0.011 ng mL-1, with linear ranges of 0.1-500 ng mL-1 and 0.05-500 ng mL-1, respectively. This aptamer engineering strategy and the developed Vap@AuNFs-Apt-LFA offer new directions for food safety and provide a reference for feasible portable detection methods.","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117100"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0