Biosensors and Bioelectronics最新文献

{"title":"Corrigendum to \"A CRISPR-Cas12a-mediated dual-mode luminescence and colorimetric nucleic acid biosensing platform based on upconversion nanozyme\" [Biosens. Bioelectron. 270 (2025) 116963].","authors":"Jiaxiang Yan, Bohan Yin, Qin Zhang, Chuanqi Li, Jiareng Chen, Yingying Huang, Jianhua Hao, Changqing Yi, Yu Zhang, Siu Hong Dexter Wong, Mo Yang","doi":"10.1016/j.bios.2025.117738","DOIUrl":"10.1016/j.bios.2025.117738","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117738"},"PeriodicalIF":10.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144566883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Development of a universal one-pot CRISPR assay based on multifunctional tagged primer eliminating unstable crRNA input and PAM dependency for point-of-care detection of bacterial infections\" [Biosens. Bioelectron. 287 (2025) 117718].","authors":"Xiaofan Zhu, Jingran Jiao, Qun Ni, Li Yao, Yule Zhang, Kaiyong Liu, Lin Huang, Qingli Bo, Panzhu Qin","doi":"10.1016/j.bios.2025.117742","DOIUrl":"10.1016/j.bios.2025.117742","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117742"},"PeriodicalIF":10.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferrocene derivatives constructed Prussian blue analogs for colorimetric detection of hydrogen peroxide and glucose.","authors":"Yue Tong, Qian Liu, Dan Yang, Chunze Zhang, Yi Xiao, Mengying Shi, Meng Meng, Huixia Di, Hongmei Gao, Rimo Xi, Yongmei Yin","doi":"10.1016/j.bios.2025.117714","DOIUrl":"10.1016/j.bios.2025.117714","url":null,"abstract":"<p><p>This study reports the rational design of ferrocene-derived Prussian blue analogs (PBAs) as a new class of nanozymes with enhanced catalytic properties. Capitalizing on its high peroxidase-like activity, we developed a colorimetric assay for detecting hydrogen peroxide (H₂O₂) and glucose, which exhibited high stability, sensitivity, and accuracy in biological samples such as serum. Furthermore, by leveraging the reversible structural dynamics of Fc(COOH)₂-PBA, we engineered a dual-mode colorimetric assay for visual quantitation of H₂O₂ and glucose in complex samples, including cells, tissues, and cerebrospinal fluid. The distinct colorimetric response of this system demonstrates promising potential for point-of-care testing applications. Our findings provide mechanistic insights into the structure-activity relationships of nanozymes and will facilitate the development of application-targeted nanozymes for disease diagnostics.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117714"},"PeriodicalIF":10.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel microneedle based transdermal fluorescent patch for the on-site and non-invasive diagnosis of Alzheimer's disease","authors":"Yingchun Zuo ,&nbsp;Shiyu He ,&nbsp;Wenmeng Zhang ,&nbsp;Yanqing Wu ,&nbsp;Huijun Qiao ,&nbsp;Xinguang Zhou ,&nbsp;Yanyan Yu","doi":"10.1016/j.bios.2025.117823","DOIUrl":"10.1016/j.bios.2025.117823","url":null,"abstract":"<div><div>Detection of biomarkers in interstitial fluid (ISF) by microneedles (MNs) has received extensive attentions as a non-invasive biosensing strategy. However, fewer work has focused on the long non-coding RNAs (lncRNAs) sensing, which are most likely present in ISF and engage in the occurrence and development of Alzheimer's disease (AD). In this work, taking adenocarcinoma transcript 1 (MALAT1) as the representative of lncRNAs, which is abnormally down-regulated in AD patients, a robust hydrogel MN array - based fluorescent patch was developed for MALAT1 by integrating a methacrylated gelatin (GelMA) hydrogel MN as the extraction tool with a strand displacement reaction (SDR) mediated fluorescent probe. Specifically, the MNs were prepared by the simple and feasible photo-crosslinking of GelMA, which displayed satisfactory swelling capability for extracting ISF and mechanical strength to penetrate the skin's cuticle layer. Subsequently, a triplex SDR amplification probe was constructed for MALAT1 sensing. By mixing with the prepolymer solution, the MNs patch loaded with the fluorescent probe was fabricated, which demonstrated excellent sensing performances in both PBS and agarose simulated skin, with lower limits of quantification (LLOQ) of 7.62 and 13.24 nM, respectively. The developed MN patch was proved highly suitable for distinguishing the down-regulated MALAT1 expressions in ISF of AD mice. This work represented the first example to correlate the changes in the levels of MALAT1 in ISF with AD pathology, providing a practical tool for the early non - invasive diagnosis of AD.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"Article 117823"},"PeriodicalIF":10.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supramolecular hydrogel mediated photoelectrochemical/electrochemiluminescence dual-mode biosensor based on TiO2/CdIn2S4/CdS for sensitive detection of enrofloxacin","authors":"Jiajie Zhang,&nbsp;Yuanzhen Ning,&nbsp;Minghao Bai,&nbsp;Guifen Jie,&nbsp;Xinxing Wang","doi":"10.1016/j.bios.2025.117817","DOIUrl":"10.1016/j.bios.2025.117817","url":null,"abstract":"<div><div>In this paper, a new photoelectrochemical (PEC) and electrochemiluminescence (ECL) dual-mode biosensor based on TiO₂/CdIn₂S₄/CdS (TO/CIS/CdS) composite and a unique supramolecular hydrogel quenching probe was constructed for sensitive detection of enrofloxacin (ENR). Firstly, TO/CIS/CdS showed excellent PEC and ECL dual signals, which was applied to dual-mode sensing and detection. By using cyclic amplification and magnetic separation techniques, target ENR was converted into a large number of product chains with ZnO nanoparticles (NPs), and acidolysis was carried out to obtain zinc ions. Then, a layer of supramolecular hydrogel with good biocompatibility and stability was formed on the electrode using the ligand interaction between adenosine monophosphate (AMP) and Zn²⁺. The poor electrical conductivity and light transmission of the hydrogel layer make it an excellent double-quenching material for PEC and ECL signals, which was used for sensitive dual-mode detection of ENR. The cyclic amplification technique in a homogeneous solution was utilized to improve the sensitivity of detection while avoiding the complex electrode-modification process, thus effectively improving efficiency and stability of the sensor. The detection range of the PEC mode can reach 0.1 pg mL^˗1 to 1 μg mL^˗1, and the limit of detection (LOD) is 35 fg mL^˗1. The detection range of the ECL mode is from 1 pg mL^˗1 to 1 μg mL^˗1, and the LOD is 0.14 pg mL^˗1. Thus, a new multifunctional biosensor utilizing TO/CIS/CS and the unique hydrogel quencher was opened up, which has great potential for application in the field of food safety and environmental monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"Article 117817"},"PeriodicalIF":10.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic self-calibration strategy based on nano-cornucopia MOFs for accurate HER-2 detection in precision breast cancer diagnosis","authors":"Mengai Yin ,&nbsp;Tong Wang ,&nbsp;Zhijie Wang ,&nbsp;Lina Lu ,&nbsp;Bowen Ding ,&nbsp;Jiadong Chi ,&nbsp;Xiangrui Wang ,&nbsp;Lan Xue ,&nbsp;Fuju Dai ,&nbsp;Bingxin Hu ,&nbsp;Jun Jiao ,&nbsp;Qiang Chen","doi":"10.1016/j.bios.2025.117813","DOIUrl":"10.1016/j.bios.2025.117813","url":null,"abstract":"<div><div>With its high porosity and tunable properties, metal-organic frameworks (MOFs) serve as nano-cornucopias, unlocking limitless possibilities in biosensing applications. Herein, we designed an ultrasensitive biosensor leveraging MOFs as a multifunctional nano-platform to implement a synergistic self-calibration strategy for the ultra-accurate quantification of breast cancer-related human epidermal growth factor receptor-2 (HER-2). This approach integrates (1) ratiometric electrochemistry for built-in correction to minimize non-specific interference and (2) dual-modal electrochemical and colorimetric detection for enhanced accuracy through cross-validation. Functionalized NH₂-UiO-66 (Zr-MOF) was employed as a carrier to incorporate a dual-layer of anthraquinone-2-carboxylic acid (AQ), creating NH₂-UiO-66@AQ, a highly electroactive nanomaterial for signal amplification. The incorporation of gold nanoparticles (AuNPs) and ionically reduced graphene oxide (IL-rGO) further enhanced conductivity and electrochemical response. Additionally, MOF@FeTCPP nanozyme was synthesized by encapsulating iron (III) meso-tetra(4-carboxyphenyl) porphyrin chloride (FeTCPP) into HKUST-1 (Cu-MOF), demonstrating exceptional peroxidase-like activity. Coupling this nanozyme with streptavidin (SA) resulted in the formation of a dual-signal nanozyme probe (DSN-Probe) capable of generating both electrochemical and colorimetric signals. The biosensor exhibited a broad detection range (100 fg/mL to 150 ng/mL) with an ultra-low limit of detection (LOD) of 28.509 fg/mL, effectively differentiating HER-2 expression in various cell lines and distinguishing HER-2 levels in serum samples from healthy individuals and breast cancer patients. Its adaptability to electrochemical workstations, microplate readers, and smartphones makes it ideal for point-of-care testing (POCT), reinforcing its potential as a powerful tool for drug mechanism studies and breast cancer diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"Article 117813"},"PeriodicalIF":10.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxygen vacancies-driven self-powered photoelectrochemical sensor for acetamiprid detection","authors":"Xiaoran Yang ,&nbsp;Xiang Ren ,&nbsp;Jingui Chen ,&nbsp;Na Song ,&nbsp;Hongmin Ma ,&nbsp;Jinxiu Zhao ,&nbsp;Qin Wei","doi":"10.1016/j.bios.2025.117814","DOIUrl":"10.1016/j.bios.2025.117814","url":null,"abstract":"<div><div>Residues of acetamiprid (ACT) in agricultural products may enter the human body with the diet and subsequently cause potential harm to humans. Therefore, it is necessary to establish a rapid and sensitive method to detect ACT. In this work, a self−powered ACT photoelectrochemical (PEC) sensor using W₁₈O₄₉/CdS as photoanode, BiOCl as photocathode, and Au@PANI as the signal amplification marker was constructed. The matched band gap structure of the W₁₈O₄₉/CdS can effectively accelerate the charge transfer, laying the foundation for a strong and stable photocurrent response at the cathode. In particular, the introduction of oxygen vacancies can induce defects on the surface of the photosensitive material, facilitating the separation of photogenerated carriers. When the target analyte ACT was introduced, the signal amplification was achieved by using ACT to compete with c−DNA for specific binding sites and inducing p−DNA−Au@PANI shedding. With the synergistic effect of the above multiple strategies, the designed sensor exhibits good linearity in the range of 100 fM ∼1 μM with a detection limit of 65.8 fM (S/N = 3). In addition, the sensor has excellent selectivity, stability, reproducibility, and also shows good analytical performance in real sample. It provides a reliable method for detection of ACT.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"Article 117814"},"PeriodicalIF":10.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D interconnected conductive network architectures of layered double hydroxides nanosheets anchored in MXene for particularly sensitive electrochemical biosensing platform","authors":"Fei Wang ,&nbsp;Yao Zhu ,&nbsp;Long Qian ,&nbsp;Yuhao Yin ,&nbsp;Ziyu Yuan ,&nbsp;Yuting Dai ,&nbsp;Tao Zhang ,&nbsp;Dongya Yang ,&nbsp;Fengxian Qiu","doi":"10.1016/j.bios.2025.117812","DOIUrl":"10.1016/j.bios.2025.117812","url":null,"abstract":"<div><div>The development of precise and sensitive electrochemical sensing methods for organophosphorus pesticides (OPs) is crucial for effective contamination regulation in food and environmental resources. In this study, a novel biosensing platform, AChE-Chit/PPy/NiFe LDH/TM/GCE (APLTMG), was constructed based on the activity inhibition mechanism of the chitosan (Chit) and acetylcholinesterase (AChE) system as the biorecognition element for the rapid detection of dimethoate. The NiFe LDHs, characterized by bimetallic active sites, are deposited onto the Ti₃C₂ (TM) substrate with superior conductivity and integrated with a three-dimensional (3D) conductive network of polypyrrole (PPy). This configuration facilitates rapid charge transfer and serves to enhance performance through a synergistic effect. On this basis, the APLTMG biosensor demonstrates exceptional sensitivity with a low detection limit (1.459 × 10⁻¹¹ mol L⁻¹), good recovery rates (97.43 %–106.92 %), and reasonable relative standard deviation (RSD) or coefficient of variation (CV) (&lt;7.8 %) for dimethoate in real samples. Besides, it is worth noting that the APLTMG exhibits the comparable repeatability, good reproducibility, stable storage performance, prominent selectivity and detection accuracy in comparison with LC-MS. This work provides valuable insights for the development of highly sensitive biosensors based on three-dimensional conductive architectures, with potential applications in ecological resource management and food safety monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"Article 117812"},"PeriodicalIF":10.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug functionalized organic electromechanical transistor for rapid and sensitive detection of C-reactive protein","authors":"Chenyang Li ,&nbsp;Chaoyi Yan ,&nbsp;Siyu Di ,&nbsp;Yu Xiao ,&nbsp;Xuefeng Zhang ,&nbsp;Ziling Jiang ,&nbsp;Guo Liu ,&nbsp;Runjie Zhang ,&nbsp;Deliu Zeng ,&nbsp;Yang Liu ,&nbsp;Ye Zou ,&nbsp;Jia Li ,&nbsp;Fengjiao Zhang","doi":"10.1016/j.bios.2025.117807","DOIUrl":"10.1016/j.bios.2025.117807","url":null,"abstract":"<div><div>C-reactive protein (CRP) is a critical biomarker for inflammation and postoperative recovery monitoring. Organic electrochemical transistor (OECT) plays a key role in CRP detection, however, with a limited sensitivity in physiological environment due to inefficient charge transfer within the Debye length. Here, we introduce rosuvastatin (RSV) drug as a novel recognition element for CRP detection in OECT biosensors. The grafted RSV molecules can inherently interact with CRP through electrostatic bonding interaction, contributing to a direct charge transfer between the captured CRP and the device within the Debye length limitation. Following this, the OECT biosensor exhibits a broad range (10⁻¹⁰ to 10⁻⁴ g/mL) for CRP quantification, with a short response time of 2 min. Moreover, the devices can realize selective CRP recognition in serum, demonstrating the great potential of drug receptors in electronic biosensor functionalization. This strategy paves a new way for developing ultrasensitive OECT biosensors towards accurate and reliable in-situ biomarker monitoring for convenient disease diagnose.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"Article 117807"},"PeriodicalIF":10.7,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-destructive monitoring of cartilage tissue engineering via near-infrared (NIR) spectroscopic assessment of culture medium","authors":"Nithin Sadeesh ,&nbsp;Harini Karunarathna ,&nbsp;Abolfazl Jalali ,&nbsp;Sanna Oikari ,&nbsp;Antti Eskelinen ,&nbsp;Arjen Gebraad ,&nbsp;Ervin Nippolainen ,&nbsp;Susanna Miettinen ,&nbsp;Isaac O. Afara","doi":"10.1016/j.bios.2025.117809","DOIUrl":"10.1016/j.bios.2025.117809","url":null,"abstract":"<div><div>Degenerative joint diseases are major contributors to pain, impaired mobility, and an increasing socioeconomic burden in aging populations. Tissue Engineering (TE) offers alternative solutions to restore joint functions. However, current TE approaches often fail to produce biocompatible, patient-specific cartilage. This could be due to the lack of a rapid, non-destructive assessment for monitoring tissue growth. Biological markers such as hyaluronan, lactate, and collagen are released in culture medium during tissue maturation, and measuring their levels indicates tissue growth and development. We performed near-infrared spectroscopy (NIRS) on conditioned medium collected during culture and differentiation of Na purified gellan gum (NaGG) embedded bone marrow stem/stromal cell (BMSC) constructs. We also employed NIR preprocessing using Nippy and an unbiased machine learning (ML) approach, where multiple ML regression models like linear regression, ridge regression, random forest regressor, and support vector regressor were used to correlate the spectral variations to the biomarkers released into culture medium. The analysis revealed strong correlations between near infrared (NIR) spectral data and biomarkers, with R² values of 0.976 for hyaluronan, 0.701 for lactate, and 0.444 for total collagen, and mean absolute percentage errors (MAPE) of 0.276, 0.090, and 0.158 respectively, showing the efficacy of NIRS in monitoring these key indicators. Feature importance further determined key regions of interest in NIR spectra for each of the biomarkers. In conclusion, this research is the first to demonstrate the potential of using NIRS for non-destructive assessment of tissue growth through the culture medium.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"Article 117809"},"PeriodicalIF":10.7,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}