Biosensors and Bioelectronics最新文献

{"title":"A borate-inspired strategy to enhance the sensitivity of fluorescent probe for NTR and hypoxia imaging dynamic in cancer cells","authors":"Yingchun Wu,&nbsp;Chunying Wei","doi":"10.1016/j.bios.2025.117567","DOIUrl":"10.1016/j.bios.2025.117567","url":null,"abstract":"<div><div>Designing highly sensitive and selective imaging probes for NTR overexpressed in cancer cells remains a great challenge. Herein, an ultra-high sensitive fluorescence probe <strong>PNO</strong> for imaging NTR in <em>in vitro</em> A549 cells was designed and synthesized. <strong>PNO</strong> is composed of 4-nitro-1,8-naphthalic anhydride and boronic acid ester groups. For comparison, another probe <strong>CNO</strong> (unmodified with boronic acid ester) was also synthesized and investigated. UV–visible absorption and fluorescence spectroscopic studies indicated that <strong>PNO</strong> exhibited an ultra-sensitive (with the detection limit (DL) of 0.543 ng/mL) and selective response to NTR. Compared to <strong>CNO</strong>, <strong>PNO</strong> possessed the higher affinity to NTR and faster enzymatic reaction rate, and the Michaelis-Menten constant (<em>K</em>_m) and maximum reaction rate (<em>V</em>_max) values were 6.199 μM and 1.641 μM s⁻¹, respectively. The molecular docking further showed that the binding energy of <strong>PNO</strong> to NTR was slightly lower than <strong>CNO</strong>, moreover, the reduced intermediate and product of <strong>PNO</strong> was easier to release from NTR, enhancing the catalytic reaction rate. Besides, <strong>PNO</strong> presented the high stability and the low cytotoxicity. Especially, <strong>PNO</strong> was able to image sensitively the fluctuations of endogenous NTR in various physiological processes. It was found that the intracellular NTR level enhanced in hypoxia, autophagy, early apoptosis, and CoCl₂-mediated activation of HIF-1α signaling pathway. On the contrary, the NTR level decreased in the late apoptosis and drug-mediated inhibition of HIF-1α signaling pathway. We anticipated that the novel design strategy of the <strong>PNO</strong> probe and the findings would provide a promising future for tumor diagnosis and treatment.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"285 ","pages":"Article 117567"},"PeriodicalIF":10.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative detection and cellular imaging of hydrogen sulfide using a SERS probe based on AuAg nanocages","authors":"Xiaoli Wang ,&nbsp;Jiale Zhang ,&nbsp;Yun Wang,&nbsp;Yang Shen,&nbsp;Yuhan Zhang,&nbsp;Yuting Zhang,&nbsp;Nandi Zhou","doi":"10.1016/j.bios.2025.117580","DOIUrl":"10.1016/j.bios.2025.117580","url":null,"abstract":"<div><div>Hydrogen sulfide (H₂S), a crucial gasotransmitter, plays an essential regulatory role in various physiological and pathological processes. There is an urgent need to develop sensitive and effective detection methods for intracellular H₂S, as abnormal H₂S levels are closely related to various diseases such as tumors. Herein, a surface-enhanced Raman scattering (SERS) probe was constructed for quantitative detection and imaging of H₂S in living cells. The SERS probe was obtained by using gold silver alloy nanocages (AuAg NCs) with superior plasmonic activity as SERS substrate, followed by modification with Raman signal molecule (4-ethynylaniline), mucin1 aptamer and polyethylene glycol. Upon exposure to H₂S, Ag in the SERS probe was rapidly and specifically converted to Ag₂S, leading to a remarkable decrease in the SERS intensity of the probe at 2010 cm⁻¹, a spectral region within the cell silent region. The developed SERS probe exhibited outstanding performances, including high sensitivity (with a detection limit as low as 0.36 nM for H₂S), remarkable selectivity, excellent stability and minimal cytotoxicity. Notably, this SERS probe had been successfully applied for the detection and imaging of both endogenous and exogenous H₂S levels in single living cells without bio-interference, highlighting its potential for precise and accurate intracellular H₂S monitoring. This advancement provides a powerful tool for studying H₂S-related physiological processes and disorders.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"285 ","pages":"Article 117580"},"PeriodicalIF":10.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted molecular rapid SERS diagnosis in clinical human serum through aptamer origami-collapsed nanofingers chip","authors":"Beijia Ji ,&nbsp;Zerui Liu ,&nbsp;Zhekai Lv ,&nbsp;Qihan Yang ,&nbsp;Jingyi Sun ,&nbsp;Guangxu Su ,&nbsp;Yuxuan Xia ,&nbsp;Xinxin Yan ,&nbsp;Junzheng Hu ,&nbsp;Pan Hu ,&nbsp;Wanwan Yi ,&nbsp;Chengyou Jia ,&nbsp;Jiangbin Wu ,&nbsp;Peng Zhan ,&nbsp;Pingheng Tan ,&nbsp;Wei Wu ,&nbsp;Fanxin Liu","doi":"10.1016/j.bios.2025.117583","DOIUrl":"10.1016/j.bios.2025.117583","url":null,"abstract":"<div><div>Surface-Enhanced Raman Scattering (SERS) offers great potential for label-free molecular diagnosis, especially in detecting disease biomarkers. However, the complexity of the biological environment in clinical human serum often significantly impairs detection accuracy. In this study, we present a highly effective SERS strategy utilizing aptamer origami-collapsed nanofingers for the precise qualitative and quantitative detection of specific targeted biomarkers in clinical serum. Here, the biomarker-specific aptamers are anchored to gold nanofingers, which then collapse during liquid evaporation, forming sub-nanometric gaps that enhance near-field strength. The serum is introduced directly into these stabilized nanofingers, where targeted biomarkers are selectively captured in aptamer hotspots, yielding pure Raman spectra of the biomarkers without interference from other serum molecules. The ratio of the biomarker's characteristic Raman peak to that of the aptamer allows for accurate quantification. This approach was validated with alpha-fetoprotein (AFP) for hepatocellular carcinoma and cardiac troponin I (cTnI) for acute myocardial infarction in clinical serum, achieving detection within 3 min. This strategy represents a significant advancement in SERS-based medical diagnostics, offering exceptional sensitivity and specificity in complex biological samples.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"285 ","pages":"Article 117583"},"PeriodicalIF":10.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interface self-shelling effect-mediated photoinduced carrier transport and multiplexed signal amplification mechanism in self-powered photoelectrochemical biosensing","authors":"Jinjie Liu ,&nbsp;Luyang Lv ,&nbsp;Dongquan Leng ,&nbsp;Yu Du ,&nbsp;Xiang Ren ,&nbsp;Hongmin Ma ,&nbsp;Tingting Wu ,&nbsp;Qin Wei","doi":"10.1016/j.bios.2025.117577","DOIUrl":"10.1016/j.bios.2025.117577","url":null,"abstract":"<div><div>In the realm of biomedical diagnostics, the development of sensitive and specific detection methods for cancer biomarkers is of paramount importance. Herein, we report on the design and implementation of a self-powered photoelectrochemical (PEC) sensor that harnesses amplified photocathode signals for the deterioration of carbohydrate antigen 125 (CA125) associated with ovarian cancer. This self-powered sensing platform integrates Cu₂O/Cu₃SnS₄ heterojunction and ZnIn₂S₄ sensitized TiO₂ with flower-like structure as photocathode and photoanode. Moreover, the PEC biosensor introduces the interface shedding effect to overcome the limitations of weak or unstable photocathode PEC signals. When MnO₂ nanoparticles are used as the quenching source, the cathode photocurrent experiences a reduction to a certain extent owing to the phenomenon of competitive light absorption. To enhance the application for efficient CA125 detection, the interface self-shelling effect is introduced. The effect is implemented through the hydrolysis reaction of Acetylcholinesterase (AChE), producing thiocholine (TCh) as the interface detachment initiator. Which resulting in the detachment of layer modifiers, including MnO₂, from the electrode surface and achieving the effect of significant enhancement of the photoelectric signal. Therefore, multiple signal amplification effects synergistically enhanced the photoelectric response. The self-powered PEC biosensing with a wide linear range of 0.001 U/mL-200 U/mL and a low detection limit of 0.32 mU/mL, which shows excellent performance in terms of sensitivity, specificity, and stability, making it a promising candidate for point-of-care diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117577"},"PeriodicalIF":10.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Air-liquid microfluidics-integrated surface-enhanced Raman spectroscopy for selective molecular adsorption and detection to achieve bacterial discrimination","authors":"Chi-Yao Ku ,&nbsp;Yu-Wei Chiang ,&nbsp;Huai-Yuan Hsu ,&nbsp;Ho-Wen Cheng ,&nbsp;Ko-Lun Chen ,&nbsp;Yin-Yi Han ,&nbsp;Juen-Kai Wang ,&nbsp;Yuh-Lin Wang ,&nbsp;Nien-Tsu Huang","doi":"10.1016/j.bios.2025.117576","DOIUrl":"10.1016/j.bios.2025.117576","url":null,"abstract":"<div><div>Bacterial discrimination is crucial for accurate microbiological diagnosis and timely antibiotic treatment. Surface-enhanced Raman spectroscopy (SERS) is an ideal technique due to its non-invasive, label-free molecular sensing capabilities. By analyzing bacterial supernatants, containing various purine derivatives, SERS can differentiate bacterial species based on their unique spectral distributions. However, the same bacterial species with different antibiotic resistance may secrete similar purine derivatives, differing only slightly in composition. Furthermore, each purine derivative may have a different molecular affinity to the SERS substrate, making it difficult to distinguish the exact molecular ratio. To improve SERS-based bacterial discrimination, we propose an air-liquid microfluidics-integrated SERS system (ALM-SERS) that selectively adsorbs and detects bacterial secretions. By taking features of precise microdroplet manipulation and contact area from microfluidics and features of selective molecular adsorption and fingerprints characterization from the SERS technique, we successfully perform a \"sequential molecular adsorption\" strategy to address the signal interference of complicated molecular mixtures in existing SERS methods. As a proof of concept, we first evaluate the molecular affinity of purine derivatives and then demonstrate the competitive analyte adsorption using adenine/cytosine and hypoxanthine/uracil sample mixtures. Finally, we tested six bacterial supernatants, including two Gram types and four strains with identical taxonomy but differing antibiotic resistance. Another six clinically isolated bacterial samples with different antibiotic resistance were also applied. Our results showed that a successful bacterial discrimination between similar species was not possible using a single SERS spectrum. In summary, the ALM-SERS system offers a powerful approach for bacterial discrimination, even when spectral differences are subtle. Beyond microbiology, this technique holds potential for analyzing complex molecular mixtures in drug development, food safety, and environmental hazard detection.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"285 ","pages":"Article 117576"},"PeriodicalIF":10.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct monitoring of electrochemical behavior of viable E. coli under various stress conditions without mediators","authors":"Xinfang Zhang ,&nbsp;Kai Zhou ,&nbsp;Xian Mao ,&nbsp;Ying Xiong ,&nbsp;Jiali Ren","doi":"10.1016/j.bios.2025.117578","DOIUrl":"10.1016/j.bios.2025.117578","url":null,"abstract":"<div><div><em>Escherichia coli</em> (<em>E. coli</em>) plays a vital role in human life and various fields, yet its naturally non-electroactive nature presents challenges for electrochemical detection. In this study, we directly monitored <em>E. coli</em>'s electrochemical behavior in an M9 medium without exogenous electron shuttles or genetic modifications, obtaining an oxidation peak at +0.35 V (vs Ag/AgCl). The electrochemical signal correlated with bacterial growth and viability. Under stress conditions (hypoxia, acid, heat, osmotic, oxidative, and metal ion stress), signal intensity correlates with the number of viable <em>E. coli</em> cells and their electron transport activity. Hydroquinone (HQ) was identified as the contribution to the signal via electrochemical analysis, Prep-HPLC, and GC-MS. This study directs the detection of quinone-related electrochemical behavior in <em>E. coli</em>, offering insights into quinone-mediated electron transfer and potential applications in food science and environmental engineering.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117578"},"PeriodicalIF":10.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Covalent organic framework-based surface plasmon-enhanced fluorescence sensing for real-time monitoring of cell apoptosis","authors":"Zhao Li ,&nbsp;Min Chen ,&nbsp;Qing-Bo Liang ,&nbsp;Xiao-Hui Pan ,&nbsp;Jia-Dai Wang ,&nbsp;Yu-Hua Weng ,&nbsp;Shuo-Hui Cao ,&nbsp;Yao-Qun Li","doi":"10.1016/j.bios.2025.117569","DOIUrl":"10.1016/j.bios.2025.117569","url":null,"abstract":"<div><div>The complex physiological environment of living organisms is a major hurdle for in situ monitoring of vital cellular activities. Here, we propose that the surface plasmon-coupled emission (SPCE) biointerface sensing system prepared by modifying covalent organic frameworks (COFs) on metal substrates, can be a powerful tool for biointerface sensing. We have successfully developed a novel pH-responsive fluorescent COF nanoprobe, where fluorophores were precisely post-modified into intrinsically enriched chemically reactive sites within the nanoporous structure. A graphene oxide-assisted assembly strategy was employed to facilitate the robust integration of COFs onto the Ag film. Remarkably, the resulting COF-modified biosensing platform achieves a 40-fold directional fluorescence enhancement in directional fluorescence, attributed to the synergistic coupling between the near-field excited fluorophore dipole, Ag nanofilm and π-conjugated graphene oxide. By precisely controlling the penetration depth of the evanescent through angular modulation of incident light, selective detection of the extracellular and intracellular information can be realized. This allows us to construct a stable, fluorescence-enhanced biosensor chip based on surface plasmon coupling for accurate in situ monitoring of extracellular pH changes during apoptosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117569"},"PeriodicalIF":10.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering of a palindrome-crosslinked DNA nanoaggregate for rapid detection of circular RNA and precise identification of lung cancer","authors":"Sirui Xiong ,&nbsp;Yun Han ,&nbsp;Mao-tao He ,&nbsp;Fei Ma ,&nbsp;Chun-yang Zhang","doi":"10.1016/j.bios.2025.117564","DOIUrl":"10.1016/j.bios.2025.117564","url":null,"abstract":"<div><div>Circular RNAs (circRNAs) are endogenous covalently closed non-coding RNAs with cell-/developmental-stage-/tissue-specific expression patterns, and they can act as the miRNA sponges and gene transcription regulatory factors to influence numerous biological processes. Herein, we develop a palindrome-crosslinked DNA nanoaggregate system to rapidly detect circRNA and precisely identify lung cancer. We utilize a self-assembled palindromic DNA nanosphere (DS) as the spatial-confinement scaffold to anchor hairpin probes (HP) for the formation of the hybrid assemblies (DSH). The presence of target circSATB2 can hybridize with the hairpin probe to expose the locked palindromic sequence, initiating the cross-linking of the palindromic ends to form a self-catenated structure through intermolecular hybridization. Then the hybridized palindromic ends serve as the self-primers to initiate extension reaction and eventually assemble into the net-like crosslinked DNA nanoaggregates, resulting in the recovery of Cy5 signals. Taking advantage of the excellent antidegradation capability and superior kinetic behavior of DSH nanostructure, high amplification efficiency of Klenow Fragment polymerase (KF)-mediated extension reaction, and signal enhancement induced by the DNA nanoaggregates, this nanosystem enables mix-and-read detection of circSATB2 within 30 min under isothermal conditions (37 °C) with a limit detection of 77.56 fM. Moreover, it is capable of measuring intracellular circSATB2 with single-cell sensitivity, exploring its biological functions, and precisely identifying different stages (I/II/III) and subtypes (IA1/IA2/IA3/IB) of lung cancers, holding great potential in early screening of lung cancers.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117564"},"PeriodicalIF":10.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)","authors":"Siyuan Liu ,&nbsp;Guohui Xiao ,&nbsp;Peixuan Li ,&nbsp;Yating Xu ,&nbsp;Xinhao Fan ,&nbsp;Lin Tian ,&nbsp;Su Zhang ,&nbsp;Guoliang Zhang","doi":"10.1016/j.bios.2025.117566","DOIUrl":"10.1016/j.bios.2025.117566","url":null,"abstract":"<div><div>Tuberculosis (TB) screening in clinical diagnosis is challenging due to issues such as sputum dependence, time-consuming procedures, and high costs. In this study, we introduce a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing), an aptamer-based CRISPR/Cas12a assay designed for the rapid and sensitive detection of <em>Mycobacterium tuberculosis</em> (Mtb) antigens from peripheral blood. Aptamer 3 (Ap3) and the aptamer-mediated probe (Aptamer-blocker 3–7) were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Ap3 demonstrated a dissociation constant (<em>K</em>d) of 8.3E-7 M with the ESAT6/CFP10 fusion proteins (EC proteins), which are produced during the replicative phase of Mtb. Upon labeling the EC proteins with Aptamer-blocker 3–7 (Ap-blocker 3–7) probe, single-stranded DNA (ssDNA) blocker 3–7 was released, thereby completing the process for RPA-based CRISPR/Cas12a fluorescence detection. After optimizing multiple parameters, CRAFT achieved a detection limit of 0.1 ag/mL EC proteins (equivalent to 3 protein particles per mL) within 120 min from plasma sample to result. The method was validated with 86 clinical plasma samples confirmed the method's high diagnostic accuracy for Mtb infection (sensitivity: 97.1 %, 95 % confidence interval (CI) [0.849–0.998]); specificity: 98.0 %, 95 % CI [0.897–0.999]), supporting its utility in early therapeutic evaluation of tuberculosis management.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"284 ","pages":"Article 117566"},"PeriodicalIF":10.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measurement of lactate concentration using a minimally invasive needle with non-planer optical waveguides","authors":"Daigo Koiwa,&nbsp;Yoichi Haga,&nbsp;Noriko Tsuruoka","doi":"10.1016/j.bios.2025.117557","DOIUrl":"10.1016/j.bios.2025.117557","url":null,"abstract":"<div><div>Traditional methods for sampling biological substance concentrations in blood are invasive and unsuitable for continuous monitoring. This paper presents a minimally invasive device equipped with optical waveguides on the surface of an acupuncture needle for measuring lactate concentration in subepidermal tissue, potentially in a continuous fashion. The device utilizes absorbance measurements, based on the correlation between absorbance and lactate concentration in tissue. The needle, with a diameter of 200 μm, exhibits good penetrability, particularly when coated with a biocompatible epoxy, which reduces insertion resistance. The experimental results demonstrate that the device can successfully measure the concentration of lactate solutions, with light intensity decreasing as the lactate concentration increases. However, challenges remain in optimizing the device to reduce optical losses and enhance measurement accuracy. Future work will focus on reducing optical loss, conducting animal experiments, and developing a miniaturized system for simultaneous measurement of multiple biological substances, aiming for practical applications in daily health monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"285 ","pages":"Article 117557"},"PeriodicalIF":10.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}