Biosensors and Bioelectronics最新文献

Cortical, striatal, and thalamic populations self-organize into a functionally connected circuit with long-term memory properties 大脑皮层、纹状体和丘脑群体自组织成具有长期记忆特性的功能连接回路

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-17 DOI: 10.1016/j.bios.2024.116840

{"title":"Cortical, striatal, and thalamic populations self-organize into a functionally connected circuit with long-term memory properties","authors":"","doi":"10.1016/j.bios.2024.116840","DOIUrl":"10.1016/j.bios.2024.116840","url":null,"abstract":"<div><div>The human brain is a complex organ with an intricate neuronal connectivity and diverse functional regions. Neurological disorders often disrupt the delicate balance among these anatomical compartments, resulting in severe impairments. The available therapeutic options constitute an incomplete solution as many patients respond partially, highlighting the need for continued research into causes and treatments. Bottom-up approaches, like <em>in vitro</em> models, offer insights into brain functions as they recreate the <em>in vivo</em> microenvironment that allows studying how specific features affect physiological and pathological conditions. In this work, we engineered the cortical-striatal-thalamic (CST) circuit, involved in many brain functions such as action initiation and selection, using a three-compartment polymeric device. We characterized the emerging spontaneous electrophysiological activity by using Micro-Electrode Arrays (MEAs). Cortical neurons exhibited complex bursting activity, which influenced the entire circuit. Striatal and thalamic neurons displayed predominantly tonic firing when isolated, while interconnections with the cortex synchronized and organized their neuronal activity, highlighting the cortical pivotal role in bursting activity and information processing. The CST circuit demonstrated self-organization abilities and displayed high entropy values, indicative of dynamic richness and information encoding potential. Furthermore, we proved the CST’s involvement in learning and memory. Our CST model provides a platform for further exploration into brain circuitry and potential therapeutic interventions, underscoring the necessity of realistic <em>in vitro</em> models to fully understand neurological diseases' pathophysiology.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NanoMIP beacons with a co-operative binding mechanism for the all-in-one detection of methamphetamine aptamer complexes 具有协同结合机制的纳米 MIP 信号信标，用于一体化检测甲基苯丙胺适配体复合物

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-15 DOI: 10.1016/j.bios.2024.116856

{"title":"NanoMIP beacons with a co-operative binding mechanism for the all-in-one detection of methamphetamine aptamer complexes","authors":"","doi":"10.1016/j.bios.2024.116856","DOIUrl":"10.1016/j.bios.2024.116856","url":null,"abstract":"<div><div>Methamphetamine is a highly addictive stimulant with significant public health implications, necessitating the development of rapid, sensitive, and reliable detection methods. Traditional analytical techniques, though accurate, often involve complex sample preparation, expensive equipment, and lengthy analysis times. This study presents the design, synthesis, and application of nanoMIP beacons with a unique co-operative binding mechanism for the detection of methamphetamine. NanoMIP beacons selective for methamphetamine/aptamer complexes were synthesized using a solid-phase synthesis method that involved the bio-conjugation of an aptamer on the stationary phase and the incubation of methamphetamine to form a methamphetamine-aptamer complex. The resultant nanoMIP beacons were eluted off the affinity column and characterised using transmission electron microscopy. The co-operative binding mechanism, which relies on the structure-switching capability of the aptamer upon analyte binding was demonstrated through comparison of the fluorescence quenching signal of a scrambled sequence and nanoNIP controls. The fluorescence quenching assay was established using a fixed optimal concentration of aptamer and varying amounts of methamphetamine. The nanoMIP beacons showed enhanced the sensitivity (LOD = 23 ± 3.5 nM) and excellent selectivity, with a 40-fold increase in quenching for methamphetamine compared to other illicit drugs. The nanoMIP beacons demonstrated acceptable sample recoveries in both urine diluent and 50% human serum. This work provides a new strategy for the development of hybrid nanoMIP/aptamer-based sensors and provides a robust analytical tool for combating methamphetamine abuse.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cascade enzyme-mimicking with spatially separated gold-ceria for dual-mode detection of superoxide anions 利用空间隔离的金铈进行级联酶模拟，实现超氧阴离子的双模式检测

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-13 DOI: 10.1016/j.bios.2024.116847

{"title":"Cascade enzyme-mimicking with spatially separated gold-ceria for dual-mode detection of superoxide anions","authors":"","doi":"10.1016/j.bios.2024.116847","DOIUrl":"10.1016/j.bios.2024.116847","url":null,"abstract":"<div><div>Metal-semiconductor nanozyme of dumbbell Au-CeO<sub>2</sub> with spatially separated heterostructure has cascade superoxide dismutase (SOD)-like and peroxidase (POD)-like activities for superoxide anions detection. It was synthesized by selective growth of CeO<sub>2</sub> at the ends of Au nanorod (Au NR). Taking advantage of the excellent local surface plasmon resonance (LSPR) effect of Au NR, the spatially separated Au-CeO<sub>2</sub> has a higher photothermal effect than the continuously growing core-shell structure of Au@CeO<sub>2</sub>. Meanwhile, the hot electrons of Au NR could transfer to CeO<sub>2</sub> under 808 nm laser irradiation, changing the ratio of Ce<sup>3+</sup>/Ce<sup>4+</sup> redox couples over CeO<sub>2</sub> and facilitating H<sub>2</sub>O<sub>2</sub> decomposition thus enhancing POD-like activity. Based on the SOD-like activity of Au-CeO<sub>2</sub>, superoxide anion (O<sub>2</sub><sup>·−</sup>) can be transformed into hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Dual-mode including absorbance and temperature sensing detection of O<sub>2</sub><sup>·−</sup>, with the detection range from nM to μM i.e., 0.1–150 μM and LOD of 0.033 μM (S/N = 3) was achieved through the cascade catalysis and photothermal effect. The as-proposed method was applicable to both cancer and normal cell samples with satisfactory accuracy and recovery.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step copper deposition-induced signal amplification for multiplex bacterial infection diagnosis on a lateral flow immunoassay device 一步式铜沉积诱导信号放大技术，用于在侧流免疫测定装置上进行多重细菌感染诊断

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-11 DOI: 10.1016/j.bios.2024.116849

{"title":"One-step copper deposition-induced signal amplification for multiplex bacterial infection diagnosis on a lateral flow immunoassay device","authors":"","doi":"10.1016/j.bios.2024.116849","DOIUrl":"10.1016/j.bios.2024.116849","url":null,"abstract":"<div><div>The lateral flow immunoassay (LFIA) is predominant in rapid diagnostic tests owing to its cost-effectiveness and operational simplicity. However, the conventional LFIA exhibits limited sensitivity and is susceptible to human variance for the result readout, impacting result interpretation. In this study, we introduced a novel one-step copper deposition-induced signal amplification lateral flow immunoassay (osa-LFIA) that markedly enhances the detection sensitivity for <em>Staphylococcus aureus</em> (protein A) and <em>Pseudomonas aeruginosa</em> (exotoxin A). Utilizing gold nanoparticles (AuNPs) as a catalyst, this approach employs ascorbic acid to reduce Cu<sup>2+</sup> to Cu<sup>0</sup>, depositing on AuNPs at the test line and amplifying the signal. A user-friendly design features a three-dimensional paper structure incorporating pre-dried reagents, enabling a streamlined, efficient testing process. The osa-LFIA significantly lowers detection limits to 3 ng mL<sup>−1</sup> for protein A and 10 ng mL<sup>−1</sup> for exotoxin A, offering a tenfold improvement over conventional LFIA. Additionally, we developed a portable grayscale detection device, achieving less than 10% error in quantitative analysis compared to the data acquired and analyzed in the lab. This entire process, from detection to signal amplification, is completed in just 20 min. For the clinical trial, we utilized the osa-LFIA to test synovial fluid samples infected with <em>Staphylococcus aureus</em>. We also successfully detected different concentrations of the exotoxin A in parallel, with a recovery value of 96%–110%. Our findings demonstrate the osa-LFIA's potential as a rapid, highly sensitive, and simple-to-use diagnostic tool for detecting various pathogens, significantly advancing the field of rapid diagnostic testing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142437714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel cobalt-based aerogels for uric acid detection in fluids at physiological pH 用于检测生理 pH 值液体中尿酸的新型钴基气凝胶

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-11 DOI: 10.1016/j.bios.2024.116850

{"title":"Novel cobalt-based aerogels for uric acid detection in fluids at physiological pH","authors":"","doi":"10.1016/j.bios.2024.116850","DOIUrl":"10.1016/j.bios.2024.116850","url":null,"abstract":"<div><div>A sensor for uric acid (UA) based on the urate oxidase enzyme (UOx) immobilized in novel Co-based aerogels with transition metals synthesized by the sol-gel method was developed and evaluated. The Co-based aerogels: Co, Ni-Co and Pd-Co were physicochemically characterized by XRD and HR-TEM. The surface area values of 53, 57 and 66 m<sup>2</sup> g-1 were determined for Co, Ni-Co and Pd-Co, respectively by N<sub>2</sub> adsorption-desorption technique. Co-based aerogels were mixed by cross-linking with UOx enzymes and electrochemically characterized in buffers at pH 7.4 and 5.6 (pH values reported for biological fluids such as blood and sweat) in the presence of different uric acid concentrations. Co-based aerogels with UOx showed improved performance as a uric acid biosensor compared to using the enzyme alone. At a pH of 7.4, a higher sensitivity of 11 μA μM<sup>−1</sup> was obtained with Pd-Co/UOx, 1.6 times higher than with UOx. At a pH value of 5.6, the highest sensitivity is achieved with Ni-Co/UOx. Stability and selectivity tests were performed in the presence of biological interferents without significant changes in the sensor. These results indicate a pleasing synergistic activity between Co-based aerogels and the enzyme.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A PLA-mediated transistor biosensor enables specific identification of tumor-derived exosomal PD-L1 聚乳酸介导的晶体管生物传感器可特异性识别肿瘤外泌体 PD-L1

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-10 DOI: 10.1016/j.bios.2024.116846

{"title":"A PLA-mediated transistor biosensor enables specific identification of tumor-derived exosomal PD-L1","authors":"","doi":"10.1016/j.bios.2024.116846","DOIUrl":"10.1016/j.bios.2024.116846","url":null,"abstract":"<div><div>The expression of programmed death ligand 1 (PD-L1) on tumor-derived exosomes (tExos) forecasts the efficacy of immunotherapy and tumor diagnosis. Due to the heterogeneity of exosomes, current detection methods face challenges in distinguishing between tumor-derived and non-tumor-derived exosome PD-L1. To address this challenge, we introduce a novel field effect transistor (FET) biosensor based on proximity ligation assay (PLA) technology. This approach uses a single probe to simultaneously recognize two biomarkers on exosomes to identify tumor-derived exosome PD-L1 (tExo<sub>-PD-L1</sub>). This method, for the first time, integrates the PLA strategy with FET technology, allowing for tracking of exosomes that co-express multiple biomarkers. In clinical diagnostics, this strategy not only significantly improves the sensitivity and specificity, but also enhances the precision and accuracy, compared to conventional approaches that identify total Exo<sub>-PD-L1</sub> or Exo<sub>-EpCAM</sub> using a single biomarker. This technology holds promise for enhancing the reliability of using exosomes as biomarkers in clinical diagnostics and further exploring the biological functions of exosomes more effectively.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative dynamics of intracellular NMN by genetically encoded biosensor 利用基因编码生物传感器定量分析细胞内 NMN 的动态变化

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-10 DOI: 10.1016/j.bios.2024.116842

引用次数: 0

A Mem-dELISA platform for dual color and ultrasensitive digital detection of colocalized proteins on extracellular vesicles 用于对细胞外囊泡上的共定位蛋白质进行双色超灵敏数字检测的 Mem-dELISA 平台

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-10 DOI: 10.1016/j.bios.2024.116848

{"title":"A Mem-dELISA platform for dual color and ultrasensitive digital detection of colocalized proteins on extracellular vesicles","authors":"","doi":"10.1016/j.bios.2024.116848","DOIUrl":"10.1016/j.bios.2024.116848","url":null,"abstract":"<div><div>Accurate, multiplex, and ultrasensitive measurement of different colocalized protein markers on individual tumor-derived extracellular vesicles (EVs) and dimerized proteins with multiple epitopes could provide insights into cancer heterogeneity, therapy management and early diagnostics that cannot be extracted from bulk methods. However, current digital protein assays lack certain features to enable robust colocalization, including multi-color detection capability, large dynamic range, and selectivity against background proteins. Here, we report a lithography-free, inexpensive (< $0.1) and ultrasensitive dual-color Membrane Digital ELISA (Mem-dELISA) platform by using track-etched polycarbonate (PCTE) membranes to overcome these shortcomings. Their through-pores remove air bubbles through wicking before they are sealed on one side by adhesion to form microwells. Immunomagnetic bead-analyte complexes and substrate solution are then loaded into the microwells from the opposite side, with >80% loading efficiency, before sealing with oil. This enables duplex digital protein colorimetric assay with beta galactosidase and alkaline phosphatase enzymes. The platform achieves 5 logs of dynamic range with a limit of detection of 10 aM for both Biotinylated β-galactosidase (B-βG) and Biotin Alkaline Phosphatase Conjugated (B-ALP) proteins. We demonstrate its potential by showing that a higher dosage of paclitaxel suppresses EpCAM-positive EVs but not GPC-1 positive EVs from breast cancer cells, a decline in chemo-resistance that cannot be detected with Western blot analysis of cell lysate. The Mem-dELISA is poised to empower researchers to conduct ultrasensitive, high throughput protein colocalization studies for disease diagnostics, treatment monitoring and biomarker discovery.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142437715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-dimensional photonic crystal acetylcholinesterase hydrogel and organohydrogel sensors for efficient detection of organophosphorus compounds 用于高效检测有机磷化合物的二维光子晶体乙酰胆碱酯酶水凝胶和有机水凝胶传感器

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-09 DOI: 10.1016/j.bios.2024.116845

{"title":"Two-dimensional photonic crystal acetylcholinesterase hydrogel and organohydrogel sensors for efficient detection of organophosphorus compounds","authors":"","doi":"10.1016/j.bios.2024.116845","DOIUrl":"10.1016/j.bios.2024.116845","url":null,"abstract":"<div><div>Sensors capable of detecting organophosphorus (OP) compounds have attracted the most attention owing to severe OP contamination worldwide. Despite many years of research, the developed OP sensors mainly focused on detecting water-soluble OPs in proper environments and the exploration of OP sensors suitable in resource-limited areas is extremely challenging. Here, a simple two-dimensional photonic crystal (2D PC) hydrogel featuring capabilities of effectively quantitative determination of OP compounds is facilely constructed by immobilizing the enzyme acetylcholinesterase (AChE) onto a bovine serum albumin (BSA) protein hydrogel. Owing to the specific interaction between AChE and OP compounds, the OP compounds are easily bound to the hydrogel, triggering volume phase transition and resulting in apparent Debye diffraction ring variations. The resulting hydrogel sensors show a limit of detection (LoD) of 2.23 nM for trichlorfon and 0.07 nM for diethyl methylphosphonate (DMPP), respectively. On the basis of the hydrogel, a responsive organohydrogel is facilely fabricated utilizing a solvent exchange strategy to meet the requirements of applications in harsh environments and detection of the non-water-soluble OP compounds. The organohydrogel sensors, however, demonstrated a LoD of 0.70 μM for trichlorfon and 4.46 μM for DMPP, respectively. This work provides new light on the development of next-generation stable, low-cost, and portable field sensing devices.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142437713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical sensors for transdermal biomarker detection: A review 用于透皮生物标记检测的光学传感器：综述

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2024-10-09 DOI: 10.1016/j.bios.2024.116844

引用次数: 0