Biosensors and Bioelectronics最新文献

{"title":"Boosting ultrasensitive electroanalytical detection of antibiotics at triphasic interface enzymatic biosensor","authors":"Rui Li ,&nbsp;Yanli Liu ,&nbsp;Jie Zhou ,&nbsp;Jie Cui ,&nbsp;Jun Chen ,&nbsp;Zhuo Li ,&nbsp;Fengxiang Chen ,&nbsp;Songqin Liu ,&nbsp;Li Mi","doi":"10.1016/j.bios.2025.117430","DOIUrl":"10.1016/j.bios.2025.117430","url":null,"abstract":"<div><div>Electroanalytical procedures are often closely bound up the gas molecules. However, the detection limitation of some electroanalytical procedures was largely limited by the lower solubility of gas molecules in liquid. To address this problem, a photoelectrochemical enzymatic biosensor with triphasic interface was designed for antibiotics detection. The hydrophobic porous carbon paper (CP) with atomic layer deposition (ALD) Zinc oxide (ZnO) film and Tungsten disulfide (WS₂) sheets were used for fixing laccase (Lac) to form Lac/WS₂/ZnO/CP, which contacted with analyst solution on one side and exposed to the gas phase directly on the other. Impressively, the catalytic activity of Lac on WS₂/ZnO/CP was promoted by adjusting the oxygen concentration from the gas phase and generated significant electrochemical response for sensitively detecting tetracycline (TC), resulting in minimum detection limit of 1.81 fM in the range of 10–200 μM. Additionally, the recovery rate of environmental samples remained at 96.07 %–104.48 %, confirming the reliability and practicality of the prepared biosensors. This strategy provided a potential method to upgrade the optical properties and enzyme activities of biosensor with eventual applications in bioanalysis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"280 ","pages":"Article 117430"},"PeriodicalIF":10.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid and ultrasensitive RPA-assisted CRISPR–Cas12a/Cas13a nucleic acid diagnostic platform with a smartphone-based portable device","authors":"Fei Hu ,&nbsp;Yunyun Zhang ,&nbsp;Yue Yang ,&nbsp;Lixin Peng ,&nbsp;Shuhui Cui ,&nbsp;Qing Ma ,&nbsp;Fangning Wang ,&nbsp;Xincheng Wang","doi":"10.1016/j.bios.2025.117428","DOIUrl":"10.1016/j.bios.2025.117428","url":null,"abstract":"<div><div>The spread of infectious diseases can be controlled by early identification of the source of infection and timely diagnosis to stop transmission. Real-time fluorescence quantitative polymerase chain reaction (PCR) is the current gold standard for pathogen diagnosis, with high detection sensitivity and accuracy. However, due to the need for specialized equipment, laboratories, and personnel, it is difficult to achieve rapid and immediate diagnosis during large-scale infectious disease outbreaks. Herein, an optimized CRISPR-based nucleic acid detection method was developed that reduces the CRISPR detection time to 15 min while maintaining high sensitivity. By using nucleic acid extraction-free and lyophilization techniques, the ‘sample-in-result-out’ detection of the two target genes of SARS-CoV-2, the human internal reference gene, and the negative quality control sample can be completed in 20 min, with a sensitivity of 0.5 copies/μL. Additionally, to facilitate the application, a smartphone-based reverse transcription-recombinase polymerase amplification (RT-RPA)-assisted CRISPR-rapid, portable nucleic acid detection device was developed, integrating functions such as heating, centrifugation, mixing, optical detection and result output. Process control, output, and uploading of detection results were conducted through smartphones. The device is not dependent on a power supply and can perform on-site rapid virus detection in resource-limited settings. Real-time uploading of results helps to rapidly implement epidemic prevention and control measures, providing an innovative means of detection, control, and prevention of virus-based infectious diseases. This important work provides a new and effective tool to manage potential future outbreaks of infectious diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"280 ","pages":"Article 117428"},"PeriodicalIF":10.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using signal off-to-on strategy for designing precise and ultrasensitive biosensor towards hepatocellular carcinoma through protein variant detection based on biocompatible bimetallic MOF","authors":"Wenwen Tu ,&nbsp;Hua Liu ,&nbsp;Junfei Wang ,&nbsp;Yu Wang ,&nbsp;Zhaoyin Wang ,&nbsp;Zhihui Dai","doi":"10.1016/j.bios.2025.117429","DOIUrl":"10.1016/j.bios.2025.117429","url":null,"abstract":"<div><div>The high mortality rate of patients with hepatocellular carcinoma (HCC) is an ever-increasing worldwide concern. Fortunately, the newest research has found that the proportion of a protein variant in total alpha-fetoprotein (AFP) over 10 % can accurately predict the incidence of HCC. Therefore, a signal off-to-on strategy was designed for developing a novel precise and ultrasensitive biosensor towards HCC through protein variant detection based on bimetallic metal-organic framework (MOF). In this study, the biocompatible Fe₂Ni-MOF was used as an electrochemically immobilized carrier, which provided abundant active sites and exhibited a synergistic effect between Fe and Ni ions for dramatically promoting the electron transfer and improving the electrochemical reduction efficiency, prominently facilitating signal amplification of the biosensing platform. Then, we designed a novel ordered labeling method to distinguish AFP-L3 from overall AFP and introduced a signal off-to-on strategy for achieving highly efficient determination of AFP-L3 %. This proposed biosensor demonstrated a satisfactory linear range, along with a very low detection limit of 69 pg/mL for AFP-L3, which was far below the medically relevant threshold level. Furthermore, the adopted biosensor presented preeminent specificity, and favorable reproducibility.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"280 ","pages":"Article 117429"},"PeriodicalIF":10.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning and statistical classification in CRISPR-Cas12a diagnostic assays","authors":"Nathan K. Khosla ,&nbsp;Jake M. Lesinski ,&nbsp;Marcus Haywood-Alexander ,&nbsp;Andrew J. deMello ,&nbsp;Daniel A. Richards","doi":"10.1016/j.bios.2025.117402","DOIUrl":"10.1016/j.bios.2025.117402","url":null,"abstract":"<div><div>CRISPR-based diagnostics have gained increasing attention as biosensing tools able to address limitations in contemporary molecular diagnostic tests. To maximize the performance of CRISPR-based assays, much effort has focused on optimizing the chemistry and biology of the biosensing reaction. However, less attention has been paid to improving the techniques used to analyze CRISPR-based diagnostic data. To date, diagnostic decisions typically involve various forms of slope-based classification. Such methods are superior to traditional methods based on assessing absolute signals, but still have limitations. Herein, we establish performance benchmarks (total accuracy, sensitivity, and specificity) using common slope-based methods. We compare the performance of these benchmark methods with three different quadratic empirical distribution function statistical tests, finding significant improvements in diagnostic speed and accuracy when applied to a clinical data set. Two of the three statistical techniques, the Kolmogorov-Smirnov and Anderson-Darling tests, report the lowest time-to-result and highest total test accuracy. Furthermore, we developed a long short-term memory recurrent neural network to classify CRISPR-biosensing data, achieving 100 % specificity on our model data set. Finally, we provide guidelines on choosing the classification method and classification method parameters that best suit a diagnostic assay's needs.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"279 ","pages":"Article 117402"},"PeriodicalIF":10.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly-sensitive and logic platform based on spatially-constrained T7 transcription enhanced Cas13a for DNA repair enzyme detection and intracellular imaging","authors":"Dongsheng Shen ,&nbsp;Hong Guo ,&nbsp;Fubin Zhang ,&nbsp;Xixi Chen ,&nbsp;Xiaowen Tong ,&nbsp;Huaifang Li ,&nbsp;Wenjun Wu ,&nbsp;Shuaikang Mei","doi":"10.1016/j.bios.2025.117406","DOIUrl":"10.1016/j.bios.2025.117406","url":null,"abstract":"<div><div>The activity of DNA repair enzymes, particularly Flap endonuclease 1 (FEN1) and apurinic/apyrimidinic endonuclease 1 (APE1), plays a critical role in disease prevention, diagnosis, and prognosis. Accurate detection of these enzymes is therefore essential. Recent advancements in CRISPR-Cas technology, particularly its programmable and trans-cleavage activity, have paved the way for the development of innovative detection methods. However, there is a need for a simple, low-background, highly sensitive detection platform with logical capabilities for FEN1 and APE1. In this study, we present a novel detection platform that integrates spatially constrained T7 transcription with the CRISPR-Cas13a system. This biosensor minimizes background interference and achieves high sensitivity, with limits of detection as low as 5 × 10⁻⁷ U/μL for FEN1 and 2 × 10⁻⁸ U/μL for APE1, making it one of the most sensitive methods available for detecting these enzymes. The platform supports both OR and logic detection, offering enhanced versatility. It demonstrates robustness by detecting FEN1 activity at concentrations as low as 1 cell/μL and screening enzyme inhibitors. Additionally, the system was successfully used for intracellular imaging of FEN1 activity in cells and reliably measured APE1 activity in ovarian tissue samples, confirming its clinical applicability. This biosensor represents a promising tool for detecting FEN1 and APE1, further expanding the potential of CRISPR-Cas13a in diagnostic applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"280 ","pages":"Article 117406"},"PeriodicalIF":10.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and sensitive biosensing of uropathogenic E. coli using plasmonic nanohole arrays on MIM: Bridging the gap between lab and clinical diagnostics","authors":"Hasan Kurt ,&nbsp;Caner Soylukan ,&nbsp;Süleyman Çelik ,&nbsp;Eda Çapkın ,&nbsp;Ibrahim Cagatay Acuner ,&nbsp;Aynur Eren Topkaya ,&nbsp;Meral Yüce","doi":"10.1016/j.bios.2025.117419","DOIUrl":"10.1016/j.bios.2025.117419","url":null,"abstract":"<div><div>This study introduces a novel biosensing platform, Plasmonic Array Nanohole Technology on Metal-Insulator-Metal (PANTOMIM), designed to overcome limitations of traditional plasmonic nanohole array biosensors. PANTOMIM utilizes a metal-insulator-metal structure as a lossy waveguide to dampen metal/substrate peaks, ensuring high extinction coefficients and spectral purity for biosensing. The architecture is optimized for the 800–850 nm wavelength range, with potential for future integration into nanophotonic devices. To demonstrate its clinical utility, we applied PANTOMIM to the detection of uropathogenic Escherichia coli (UPEC) in urine samples. This approach addresses the need for rapid diagnosis of urinary tract infections, providing results in 15 min and requiring minimal sample preparation. The efficacy of the technology was validated in a clinical setting with a cohort of 100 patients, showcasing its potential to revolutionize the detection of UPEC. PANTOMIM combines the advantages of plasmonic nanohole arrays, including tunable periodicity, coupled plasmonic response, and extraordinary optical transmission, while mitigating the challenges associated with thin-film plasmonic metals. This innovation paves the way for integrated nanoplasmonic biosensors for point-of-care diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"280 ","pages":"Article 117419"},"PeriodicalIF":10.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive detection of tetracycline using the disruption of crosslink-enhanced emission and inner-filter effect-induced phosphorescence quenching of carbonized polymer dots","authors":"Cheng-Yu Zhao ,&nbsp;Wei-Bin Tseng ,&nbsp;Kai-Hsin Hung ,&nbsp;Wei-Lung Tseng","doi":"10.1016/j.bios.2025.117409","DOIUrl":"10.1016/j.bios.2025.117409","url":null,"abstract":"<div><div>Accumulation of tetracycline (TC) in the environment and food may lead to potential health risks and the emergence of antibiotic-resistant bacteria. To meet the demand for sensitivity, ease of use, and portability in detecting TCs, we fabricated a green phosphorescent film consisting of crosslinked polymer-integrated carbon dots (named carbonized polymer dots, CPD) and polyvinyl alcohol (PVA) polymers for ultrasensitive sensing of TCs <em>via</em> the inner filter effect-mediated phosphorescence quenching and the disruption of the crosslink-enhanced emission (CEE) effect by TC. To create polymer structures on the carbon dots for interaction with PVA, CPDs were synthesized <em>via</em> low-temperature hydrothermal treatment using citric acid and cysteine. Compared to products with oxidized sulfur or no sulfur doping, the incorporation of nitrogen and sulfur in CPDs was found to effectively facilitate intersystem crossing, significantly enhancing phosphorescence. By measuring the phosphorescence properties of compounds inside and outside the dialysis bag at different dialysis times, we confirmed that crosslinking interactions between CPD and PVA polymers can create a rigid environment to amplify the phosphorescence of sub-luminophores (e.g., hydroxyl, carboxyl, and amino groups) through the CEE effect. These features make the CPD/PVA film an effective tool for phosphorescence turn-off detection of TC, offering a wide linear detection range (1 nM–1 mM), a low limit of detection (0.7 nM), and good selectivity over potential interfering substances, such as metal ions, amino acids, fatty acids, and lactose. Our finding indicates that the TC-triggered phosphorescence quenching of the CPD/PVA film originates from TC-mediated IFE effect and TC-disrupted CEE effect. The CPD/PVA film was shown to establish a linear calibration curve to quantify TC in drinking water and milk samples with good recoveries (84 %–120 %).</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"279 ","pages":"Article 117409"},"PeriodicalIF":10.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A telomerase-enhanced homogeneous cascade amplification strategy designed for highly sensitive electrochemical detection of microRNA","authors":"Sha Yu ,&nbsp;Shaodi Zhao ,&nbsp;Yibo Liu ,&nbsp;Tongnian Gu ,&nbsp;Shao-Hua Wen ,&nbsp;Junping Ma ,&nbsp;Yuan Dang ,&nbsp;Jun-Jie Zhu ,&nbsp;Yuanzhen Zhou","doi":"10.1016/j.bios.2025.117422","DOIUrl":"10.1016/j.bios.2025.117422","url":null,"abstract":"<div><div>Highly sensitive and specific detection of microRNAs (miRNAs) is vital for cancer early diagnosis. In this work, we have proposed a telomerase-enhanced homogeneous cascade amplification strategy for high-performance electrochemical detection of miRNA-21 (miR-21). The target miRNA is first transcribed and amplified into massive single-stranded output DNA fragments through the endonucleases-assisted primary amplification element. Then, the output DNAs can activate the telomerase-promoted entropy-driven DNA catalytic (EDC) circuit, which can significantly improve the amplification efficiency and release a mass of linker DNAs, achieving the secondary amplification of miR-21. Finally, the G-quadruplex loaded with plenty of electroactive substances can be captured on the electrode via the linker DNAs for highly sensitive detection of miR-21. The fabricated electrochemical biosensor exhibits a broad linear range from 1 aM to 1 nM with the detection limit of 0.36 aM. The exceptional sensitivity and specificity endow this biosensor with the ability to discriminate miR-21 from the interference miRNAs and proteins. In addition, the biosensor has been utilized to analyze miR-21 expression levels in human serum and diverse cell lysates, demonstrating its practicability in real sample analysis. Therefore, our designed electrochemical biosensor will have huge potential in analysis of cancer-related miRNA and early cancer diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"279 ","pages":"Article 117422"},"PeriodicalIF":10.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two Birds with One Stone: Fe-DNA nanospheres produced via coordination-propelled self-assembly with excellent peroxidase-like property for versatile ratiometric fluorescent assay and cellular imaging","authors":"Yuwei Zhang ,&nbsp;Jiawen Han ,&nbsp;Shuai Qin ,&nbsp;Juan Wang ,&nbsp;Zhihua Lv ,&nbsp;Shaojun Dong ,&nbsp;Daoqing Fan","doi":"10.1016/j.bios.2025.117424","DOIUrl":"10.1016/j.bios.2025.117424","url":null,"abstract":"<div><div>Exploring novel versatile nanozymes for multi-signal biosensing and cellular application is one of the most promising directions to meet the diversified requirements in this field. Herein, by harnessing coordination-propelled self-assembly between Fe (II) and DNAs, we prepared Fe-DNA nanospheres (Fe-DNA NSs) via a cost-effective one-step hydrothermal method, and pioneered the application of its excellent POD-mimicking property to fluorescent substrates. Initially, we investigated its enzyme-like activity using TMB as canonical colorimetric substrate and screened its catalytic oxidation effects towards different fluorescent substrates, such as T-HCl, AR, OPD and Sc, respectively. Afterwards, by virtue of the contrary fluorescent changes of Sc (decreased FI₄₆₅) and OPD (increased FI₅₆₂) and the cooperative effects of FRET/IFE between them, we devised the first universal Fe-DNA nanospheres-based ratiometric fluorescent (RF) platform. Taking H₂O₂ and glucose as model targets, two RF biosensors based on the alternative direct-nanozyme-catalysis and enzyme/nanozyme-tandem-catalysis were rationally fabricated, respectively. And we further exploited them to evaluate the quality of commercial contact lens care solution, and sensitively determine the blood glucose level of human. Moreover, corresponding cytotoxicity experiments adequately proved the superior biocompatibility of Fe-DNA NSs over most inorganic nanozymes. Furthermore, taking Cy5-labelled A20 strands as templates, we synthesized small-sized (∼60 nm) Fe-DNA fluorescent nanozyme and achieved efficient cellular delivery/imaging. This work not only offered a valid prototype for operating multi-signal-responsive nanozymatic biosensors, but also opened unique avenues for the bio-applications of nucleic acids-originated fluorescent nanozymes in cellular imaging and biotherapy.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"279 ","pages":"Article 117424"},"PeriodicalIF":10.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collaborative light-off and light-on bacterial luciferase biosensors: Innovative approach for rapid contaminant detection","authors":"Jun Yang ,&nbsp;Yongwei Zhang ,&nbsp;Guangjuan Luo ,&nbsp;Suiping Zheng ,&nbsp;Shuangyan Han ,&nbsp;Shuli Liang ,&nbsp;Ying Lin","doi":"10.1016/j.bios.2025.117369","DOIUrl":"10.1016/j.bios.2025.117369","url":null,"abstract":"<div><div>Bioluminescence-based light-off and light-on biosensors are widely used in environmental monitoring due to their rapid, cost-effective, real-time, and easy operation. However, stability and sensitivity issues in detecting real samples remain challenging. This study introduces a novel approach utilizing combined light-off and light-on biosensors for rapid and sensitive contaminants detection within 45 min in real samples. First, a recombinase-based state machine (RSM) was used to construct light-off RSM biosensors (light-off RSMs) for continuously strong light emission and overexpressing of outer membrane porins OmpC and OmpF enhanced their sensitivity to toxic contaminants. Additionally, a new experimental protocol containing the cell culture, collection, preparation, and the contaminant measurement was established for bioluminescent light-on whole-cell biosensors (light-on WCBs) in contaminant detection, initially developed using cadmium (Cd) and later applied to lead (Pb) and mercury (Hg). For Cd light-on WCBs, overexpressing OmpC and knocking out the contaminant exporter ZntA enhanced the accumulation of intracellular Cd in WCB cells, resulting in increased sensitivity to low concentrations of contaminants. Further, metabolic modifications in light-on WCBs significantly boosted luminescence. These genetic modified bacterial strains, whether freshly harvested or as freeze-dried powders, showed rapid luminescent responses to contaminants in the picomolar (pM) to nanomolar (nM) range within 45 min. Finally, the combined use of light-off RSMs and light-on WCBs successfully assessed toxicity and detected specific contaminants in real environmental and food samples. These strategies could be applied to developing other bacterial luciferase-based biosensors and even other types such as colorimetric biosensors.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"280 ","pages":"Article 117369"},"PeriodicalIF":10.7,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}