Biosensors and Bioelectronics最新文献

{"title":"Construction of nanozyme based with mixed valence manganese oxide loaded on defective metal-organic frameworks for sensitive detection of biomarker procalcitonin","authors":"Suimin Deng ,&nbsp;Yun Hao ,&nbsp;Lina Yang ,&nbsp;Tongtong Yu ,&nbsp;Xiangfeng Wang ,&nbsp;Hailing Liu ,&nbsp;Yuan Liu ,&nbsp;Mengxia Xie","doi":"10.1016/j.bios.2025.117339","DOIUrl":"10.1016/j.bios.2025.117339","url":null,"abstract":"<div><div>Nanozymes possess the advantages of high stability, adjustable catalytic activity and simple preparation processes, which position them as a promising alternative to natural enzymes. In this work, an oxidase-like nanozyme has been prepared by loading mixed valence manganese oxides (Mn_xO_y) on defective PCN-224 MOFs (dPCN). Dodecanoic acid was utilized to introduce abundant mesoporous defects into the dPCN, allowing manganese oxide to grow in situ on the surface and within the pores. The mixed valence state of manganese oxides endowed the Mn_xO_y@dPCN nanozyme (MdP) with redox and catalytic properties, and the high oxidase-like catalytic performance of MdP for TMB substrate also originated from its favorable electrical conductivity and affinity to the substrate. The reactive oxygen species of the catalytic reaction were mainly singlet oxygen (¹O₂) and peroxyl radicals (·O₂⁻). Without the existence of hydrogen peroxide (H₂O₂), the nanozyme can rapidly and efficiently oxidize TMB substrate into blue oxidation state, which has strong absorbance at 650 nm. An immunosensor for detecting biomarker procalcitonin (PCT) in human serum samples has been established based on the high catalytic property of MdP nanozyme. The immunoassay for PCT has satisfactory accuracy and repeatability, and its linear detection range can reach to be 0.05–100 ng mL⁻¹ with a limit of detection (LOD) of 0.011 ng mL⁻¹. The result affords a promising idea to construct oxidase-like nanozyme, and provides a method for sensitive determination of PCT in complex matrices.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"278 ","pages":"Article 117339"},"PeriodicalIF":10.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Reactive EEG biomarkers for diagnosis and prognosis of Alzheimer's disease and mild cognitive impairment\" [Biosens. Bioelectron. 273 (2025) 117181].","authors":"Soonhyouk Lee, Changtae Hahn, Eunyoung Seong, Hak Soo Choi","doi":"10.1016/j.bios.2025.117348","DOIUrl":"https://doi.org/10.1016/j.bios.2025.117348","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117348"},"PeriodicalIF":10.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrosynthesis of molybdenum carbide-doped epitope-imprinted conductive polymers for the determination of soluble suppression of tumorigenicity 2 with a field effect transistor platform","authors":"Mei-Hwa Lee ,&nbsp;Kai-Hsi Liu ,&nbsp;James L. Thomas ,&nbsp;Chen-Yuan Chen ,&nbsp;Chuen-Yau Chen ,&nbsp;David Jui-Yang Feng ,&nbsp;Hung-Yin Lin","doi":"10.1016/j.bios.2025.117353","DOIUrl":"10.1016/j.bios.2025.117353","url":null,"abstract":"<div><div>In this work, several peptides of soluble suppression of tumorigenicity 2 (sST2) were screened, synthesized, and then imprinted with electropolymerization onto poly(aniline-<em>co</em>-3-aminobenzenesulfonic acid), poly(AN-<em>co</em>-MSAN). Three MXenes, including titanium or molybdenum carbides, were also doped within the conductive polymer film to enhance the electrochemical response. The electrodes were employed as the extended gate in a field-effect transistor (EG-FET) platform. The sensing range and limit of detection for sST2 were found to be from 1.0 fg/mL to 100.0 pg/mL and 0.05 fg/mL, respectively. Importantly, incorporation of a Mo-based MXene increased the responsivity of the FET sensor by a factor of about 1.5 at pg/mL to ng/mL sST2 concentrations. This study demonstrated the potential utility of an sST2-imprinted electrochemical sensor for diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"278 ","pages":"Article 117353"},"PeriodicalIF":10.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-powered PEC platform with large and stable photocurrent for blocker-free sensitive assay of Caspase-3 activity based on CdIn2S4/CdS QDs anode and NH2-MIL-125(Ti)@MAPbI3/Au NPs cathode","authors":"Guizhen Luo,&nbsp;Yuyang Sun,&nbsp;Cuicui Du,&nbsp;Xiaohua Zhang,&nbsp;Jinhua Chen","doi":"10.1016/j.bios.2025.117350","DOIUrl":"10.1016/j.bios.2025.117350","url":null,"abstract":"<div><div>The diagnosis of apoptosis is of particular importance for assessing apoptosis-related disease progression and improving the therapy efficiency. Caspase-3 is the most frequently activated cysteine protease and a key mediator of cell apoptosis, therefore, its activity assay is vital. Here, by encapsulating of MAPbI₃ in NH₂-MIL-125(Ti) and constructing “Z-scheme” structure between CdIn₂S₄ microspheres and CdS quantum dots (QDs) to obtain high-photoelectrochemical (PEC)-stability and large-photocurrent NH₂-MIL-125(Ti)@MAPbI₃/Au NPs photocathode and CdIn₂S₄/CdS QDs photoanode, respectively, a new dual-photoelectrode self-powered PEC platform was constructed for highly sensitive and blocker-free assay of caspase-3 activity. The designed negatively-charged CC-DEVD-peptide chains were immobilized on the positive-charged NH₂-MIL-125(Ti)@MAPbI₃/Au NPs hybrids in flat-lying state due to the electrostatic interaction and double Au-S bonds to maximally suppress the photocurrent signal and to make blockers be not needed. When caspase-3 was present, the CC-DEVD-peptide chains were specifically recognized and cleaved, and only a short Cys-Cys dipeptide remained on the NH₂-MIL-125(Ti)@MAPbI₃/Au NPs/ITO photocathode, leading to an obviously enhanced photocurrent. Due to the good PEC properties and the matched energy levels of CdIn₂S₄/CdS QDs and NH₂-MIL-125(Ti)@MAPbI₃/Au NPs, the developed dual-photoelectrode self-powered PEC platform had large open circuit voltage and photocurrent, and the caspase-3 activity was sensitively assayed (linear range, 0.1 pg mL⁻¹ – 1.0 μg mL⁻¹; detection limit, 5.7 fg mL⁻¹), implying its promising applications in assessment of disease progression and therapy efficiency associated with apoptosis, and rapid screening of caspase-3-related drugs.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"278 ","pages":"Article 117350"},"PeriodicalIF":10.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive and multiplex SERS immunoassay for stroke subtype-specific biomarkers based on graphene oxide-supported nanofilms coated by roughened nanoboxes with extensive high-density hotspots","authors":"Wei-Bin Wang ,&nbsp;Ya-Fei Wang ,&nbsp;Jian-Jun Li ,&nbsp;Jing-Yuan Wang ,&nbsp;Guo-Jun Weng ,&nbsp;Jian Zhu ,&nbsp;Jun-Wu Zhao","doi":"10.1016/j.bios.2025.117352","DOIUrl":"10.1016/j.bios.2025.117352","url":null,"abstract":"<div><div>Herein, we fabricate the graphene oxide-supported nanofilms coated by roughened nanoboxes (GO@AuAgRNB) for the ultrasensitive and simultaneous determination of multiple stroke subtype-specific biomarkers. Initially, Au-Ag roughened nanobox (AuAgRNB) with abundant coupling and tip hotspots is prepared by the partial surface passivation strategy. AuAgRNB is uniformly, densely and firmly assembled onto graphene oxide (GO) by metal-sulfur bonds, generating extensive high-density hotspots. Owing to electromagnetic and chemical enhancement, the surface enhanced Raman scattering (SERS) activity of GO@AuAgRNB is greatly improved with the enhancement factor of 5.78 × 10⁷. Combined with magnetic bead, GO@AuAgRNB was employed to develop a SERS-based immunoassay platform for the simultaneous detection of glial fibrillary acidic protein (GFAP) and retinol binding protein 4 (RBP₄). The platform demonstrates ultra-sensitivity with detection ranges of 0.1 pg/mL-0.1 μg/mL and limits of detection of 0.16 pg/mL for GFAP and 0.10 pg/mL for RBP₄. Furthermore, the platform provides superior anti-interference properties, accuracy, and capability for simultaneous detection and practical application. In the detection of clinical patient samples, the receiver operating characteristic curve analysis shows that cut-off values (RBP₄ = 13.51 μg/mL and GFAP = 2.07 ng/mL) can reliably differentiate patients with ischaemic and haemorrhagic stroke. Overall, the ultrasensitive and multiplex immunoassay platform based on GO@AuAgRNB demonstrates high potential in the clinical diagnosis of stroke subtypes.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"278 ","pages":"Article 117352"},"PeriodicalIF":10.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing bifunctional nanozyme with potent catalytic and signal amplification for innovating electrochemical immunoassay.","authors":"Shuyun Chen, Shuo Tian, Yunsen Wang, Meijin Li, Dianping Tang","doi":"10.1016/j.bios.2025.117340","DOIUrl":"https://doi.org/10.1016/j.bios.2025.117340","url":null,"abstract":"<p><p>Nanozyme-based electrochemical biosensors have emerged as an alternative to enzyme-based biosensors for next-generation bioanalysis. However, potential antibody modifications limit the catalytic sites of the nanozyme, thereby reducing sensor sensitivity. Here, a sensitive method for determining carcinoembryonic antigen (CEA) was developed. It involved coupling a cascade enzyme - enzyme - like catalytic reaction using Fe - Co Prussian blue analog nanozymes with high peroxidase - like activity (79.42 U mg^-1). Briefly, the transduction of biological signals to chemical signals was achieved through the strategy centered on catalytic electroactive probes. Thereafter, with the assistance of the microelectrochemical workstation, the output of signals was realized. The platform exhibited an ultra-wide range of 0.020-100 ng mL^-1 and a detection limit of 0.013 ng mL^-1 CEA, which was mainly attributed to the excellent peroxidase activity, good conductivity, and synergistic amplification of current signals of synthesized nanozymes. In addition, the modification-free features greatly reduced the complexity of the bioassay and significantly improves its portability and cost-effectiveness. Overall, this study advances the development of nanozymes and their electrochemical biosensing applications and is expected to extend to the development of miniaturized devices in direct detection environments.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117340"},"PeriodicalIF":10.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo monitoring of endogenous hydrogen sulfide and evaluation of natural protectants in liver injury mice using a highly selective bioluminescent probe","authors":"Chong Hu ,&nbsp;Zeping Yang ,&nbsp;Xiaorui Shi ,&nbsp;Yaru Xue ,&nbsp;Liyu Huang ,&nbsp;Chu Tang ,&nbsp;Fu Wang","doi":"10.1016/j.bios.2025.117343","DOIUrl":"10.1016/j.bios.2025.117343","url":null,"abstract":"<div><div>Hydrogen sulfide (H₂S) is an essential endogenous gasotransmitter that can regulate a wide range of physiological processes. However, overproduction of H₂S is toxic to humans and causes liver injury, cardiovascular diseases, central nervous system-related disease, diabetes, even cancer. Hence, designing efficient imaging probes for real-time monitoring of the alterations in endogenous H₂S is a viable tactic for accurate diagnosis of these diseases. In this work, a bioluminescence (BL) probe, namely <strong>Luc</strong>-<strong>H</strong>_{<strong>2</strong>}<strong>S</strong>, has been developed to achieve H₂S detection <em>in vitro</em> and <em>in vivo</em>. This sensing probe enables a selective BL turn-on response to H₂S, and showcases excellent sensitivity with a low detection limit (LOD = 0.337 μM). Furthermore, <strong>Luc</strong>-<strong>H</strong>_{<strong>2</strong>}<strong>S</strong> has been successfully applied in BL imaging of endogenous H₂S in cells, tumor-bearing mice and drug-induced liver injury mice. More importantly, <strong>Luc</strong>-<strong>H</strong>_{<strong>2</strong>}<strong>S</strong> is utilized to accurately evaluate the protective effects of natural products against alcohol-induced liver injury through monitoring of the H₂S fluctuations. We envision that <strong>Luc</strong>-<strong>H</strong>_{<strong>2</strong>}<strong>S</strong> holds promise as a powerful imaging tool for diagnosis of H₂S-mediated diseases and evaluation of drug therapy.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"278 ","pages":"Article 117343"},"PeriodicalIF":10.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-functional needle-based VOC sensing platform for rapid vegetable phenotypic classification","authors":"Oindrila Hossain ,&nbsp;Yan Wang ,&nbsp;Mingzhuo Li ,&nbsp;Belinda Mativenga ,&nbsp;Sina Jamalzadegan ,&nbsp;Noor Mohammad ,&nbsp;Alireza Velayati ,&nbsp;Aditi Dey Poonam ,&nbsp;Qingshan Wei","doi":"10.1016/j.bios.2025.117341","DOIUrl":"10.1016/j.bios.2025.117341","url":null,"abstract":"<div><div>Volatile organic compounds (VOCs) are common constituents of fruits, vegetables, and crops, and are closely associated with their quality attributes, such as firmness, sugar level, ripeness, translucency, and pungency levels. While VOCs are vital for assessing vegetable quality and phenotypic classification, traditional detection methods, such as Gas Chromatography-Mass Spectrometry (GC-MS) and Proton Transfer Reaction Mass Spectrometry (PTR-MS) are limited by expensive equipment, complex sample preparation, and slow turnaround time. Additionally, the transient nature of VOCs complicates their detection using these methods. Here, we developed a paper-based colorimetric sensor array combined with needles that could: 1) induce vegetable VOC release in a minimally invasive fashion, and 2) analyze VOCs <em>in situ</em> with a smartphone reader device. The needle sampling device helped release specific VOCs from the studied vegetables that usually require mechanic stimulation, while maintaining the vegetable viability. On the other hand, the colorimetric sensor array was optimized for sulfur compound-based VOCs with a limit of detection (LOD) in the 1–25 ppm range, and classified fourteen different vegetable VOCs, including sulfoxides, sulfides, mercaptans, thiophenes, and aldehydes. By combining principal components analysis (PCA) analysis, the integrated sensor platform proficiently discriminated between four vegetable subtypes originating from two major categories within 2 min of testing time. Additionally, the sensor demonstrates the capability to distinguish between different types of tested fruits and vegetables, including garlic, green pepper, and nectarine. This rapid and minimally invasive sensing technology holds great promise for conducting field-based vegetable quality monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"278 ","pages":"Article 117341"},"PeriodicalIF":10.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a highly sensitive, high-throughput and automated CRISPR-based device for the contamination-free pathogen detection.","authors":"Hui Ge, Junya Feng, Libing Huang, Zhongqin Luo, Houyu Ling, Li Ma, Miao Wang, Haoxiang Chen, Lei Ren","doi":"10.1016/j.bios.2025.117323","DOIUrl":"https://doi.org/10.1016/j.bios.2025.117323","url":null,"abstract":"<p><p>Rapid, portable, and contamination-resistant nucleic acid detection methods are necessary due to the threat posed by emerging viruses to public health and agricultural output. We establish CARE (CRISPR-associated airtight real-time electronic diagnostic device), a novel platform that combines CRISPR-Cas12a with a hermetically sealed microfluidic chip to overcome the limitations of present technologies, which struggle to balance sensitivity, multiplexing, and field applicability. By combining isothermal amplification and CRISPR detection within a hermetically sealed microfluidic chip, CARE eliminates the risk of nucleic acid aerosol contamination while enabling simultaneous high-throughput analysis of seven pathogens. The device is complemented by a user-friendly nucleic acid quantification App, enabling rapid and precise analysis. The RPA-CRISPR/Cas12a system demonstrates exceptional sensitivity, detecting as few as 1 copy μL^-1 (single-plex) and 10-10² copies μL^-1 (multiplexed), with real-sample performance matching gold-standard methods. CARE represents a significant advancement in CRISPR-based diagnostics, offering a robust, portable solution for on-site pathogen detection in food and agricultural applications.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117323"},"PeriodicalIF":10.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical pan-variant detection of SARS-CoV-2 through host cell receptor-mimicking molecular recognition.","authors":"Minjong Lee, Ji-Soo Kwon, Sung-Han Kim, Sungwook Woo, Seung Soo Oh","doi":"10.1016/j.bios.2025.117311","DOIUrl":"https://doi.org/10.1016/j.bios.2025.117311","url":null,"abstract":"<p><p>The persistent emergence of new SARS-CoV-2 variants has presented significant challenges to vaccines and antiviral therapeutics, highlighting the need for the development of methods that ensure variant-independent responses. This study introduces a unique sensor capable of electrochemically detecting SARS-CoV-2 across a wide range of variants. The comprehensive detection is achieved by using a peptide-DNA hybrid, R7-02, as the capture probe, mimicking the binding interface between a SARS-CoV-2 spike protein and a host cell receptor, hACE2. Since the first step of viral infection is the binding of the spike protein to hACE2 regardless of variant type, the hACE2-mimicking probe can naturally acquire the pan-variant recognition capability. In constructing the sensor, the R7-02 probes are positioned on electrodes via a tetrahedral DNA nanostructure for enhanced detection efficiency. Since R7-02 directly captures the externally-exposed spike protein, our approach does not require sample pretreatments, such as virus particle lysis, unlike conventional diagnostic methods. The R7-02-embedded sensor demonstrated high sensitivity towards Omicron and its major subvariants-commonly known as 'stealth Omicron' (BA.5, BA.2.75, BQ.1.1, and XBB.1.5)-with a detection limit as low as 811.9 pM, along with robust specificity for SARS-CoV-2 against influenza and other human coronaviruses. The sensor also successfully detected SARS-CoV-2 directly from non-treated saliva samples of COVID-19-positive patients. Given the comprehensive and sensitive detection capability, combined with its simple operation, our receptor-mimicking probe-based electrochemical sensor holds the potential to be a sustainable and effective point-of-care diagnostic tool, offering a promising solution to the constant challenges posed by the endemic presence of SARS-CoV-2.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117311"},"PeriodicalIF":10.7,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}