Biosensors and Bioelectronics最新文献

Machine learning-assisted Eu-MOF fluorescent material for simultaneous monitoring and removal of malachite green 机器学习辅助Eu-MOF荧光材料同时监测和去除孔雀石绿

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-28 DOI: 10.1016/j.bios.2025.117737

Yi-Fan Xia, Akimana Sandra, Hua Yu, Hou-Qun Yuan, Zhi-Qiang Cai, Ling-Yi Guo, Yuan-Lei Zhang, Guang-Ming Bao

{"title":"Machine learning-assisted Eu-MOF fluorescent material for simultaneous monitoring and removal of malachite green","authors":"Yi-Fan Xia, Akimana Sandra, Hua Yu, Hou-Qun Yuan, Zhi-Qiang Cai, Ling-Yi Guo, Yuan-Lei Zhang, Guang-Ming Bao","doi":"10.1016/j.bios.2025.117737","DOIUrl":"10.1016/j.bios.2025.117737","url":null,"abstract":"<div><div>Many countries prohibit the use of malachite green (MG) in aquaculture, owing to its high carcinogenicity, teratogenicity, and high tendency to leave residues. Therefore, it is crucial to develop materials that can simultaneously monitor and remove MG. In this study, a stable fluorescent material (<strong>EuUQCA</strong>) was constructed as a dual-functional platform for the simultaneous monitoring and removal of MG from water. <strong>EuUQCA</strong> enabled ultrahigh-sensitivity detection of MG (with a detection limit as low as 16.8 nM) through fluorescence quenching response. The introduction of MG caused a distinct change in fluorescence color, and a paper sensor based on <strong>EuUQCA</strong> utilized the color change to enable portable and visual detection of MG. A fast and portable semiquantitative method for sensing MG in aquatic environments was established by analyzing RGB values using an integrated smartphone. Moreover, an online data analysis model was developed based on fingerprint extraction from paper sensor images and machine learning algorithms; the model was rigorously cross-validated to enhance the precision and accuracy of the visual determination of MG. Additionally, <strong>EuUQCA</strong> powder exhibited excellent adsorption capacity for MG. Kinetic studies indicated that adsorption followed a pseudo-second-order model, whereas the equilibrium data were best described by the Freundlich isotherm. This work introduces a dual-functional material that integrates simultaneous detection and adsorption of MG with machine learning-assisted portable sensors; this system can not only improve the detection performance, but also promote the intelligent development of analytic technologies, with broad application prospects in the environmental monitoring and food safety fields.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117737"},"PeriodicalIF":10.7,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wearable electrochemical aptasensor based on MXene@gold nanoparticles for non-invasive sweat cortisol detection 基于MXene@gold纳米颗粒的可穿戴电化学感应传感器用于无创汗液皮质醇检测

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-27 DOI: 10.1016/j.bios.2025.117736

Weilong Tu , Meng Sun , Tianyi Lu , Yuxian Chen , Yuxuan Zhou , Cong Zhang , Zhonghua Ni , Xiao Li , Tao Hu

{"title":"Wearable electrochemical aptasensor based on MXene@gold nanoparticles for non-invasive sweat cortisol detection","authors":"Weilong Tu , Meng Sun , Tianyi Lu , Yuxian Chen , Yuxuan Zhou , Cong Zhang , Zhonghua Ni , Xiao Li , Tao Hu","doi":"10.1016/j.bios.2025.117736","DOIUrl":"10.1016/j.bios.2025.117736","url":null,"abstract":"<div><div>Chronic stress exerts detrimental effects on both physical and mental wellbeing, thereby necessitating the quantitative and real-time monitoring of stress biomarkers, such as cortisol. To address this, a non-invasive, wearable electrochemical aptasensor based on MXene and gold nanoparticles (Mxene/AuNPs) modified screen-printed electrodes (SPEs) is proposed to detect cortisol in human sweat. Conformationally altered aptamers, with methylene blue (MB) as an electrochemical indicator, are linked to AuNPs <em>via</em> gold-sulfur bonds to capture cortisol. Mxene/AuNPs not only increase the specific surface area of the working electrode but also facilitate the immobilization of the aptamer. Moreover, an integrated microfluidic sampler is integrated with this aptasensor to ensure efficient sweat collection, which could effectively prevent sweat evaporation and contamination. Finally, the performance of this aptasensor is evaluated through the differential pulse voltammetry (DPV) technique, exhibiting high specificity ranging from 0.5 to 500 ng/ml (1.38–1379 nM) with a low detection limit of 0.1 ng/ml. This work demonstrates a practical strategy for developing cost-effective and scalable wearable sensors for sweat-based biomarker detection.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117736"},"PeriodicalIF":10.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumor microenvironment-responsive dynamic self-assembly of DNAzyme nanowires for high-contrast imaging of multiple messenger RNAs and chemotherapy 肿瘤微环境响应动态自组装DNAzyme纳米线用于多信使rna的高对比度成像和化疗

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-27 DOI: 10.1016/j.bios.2025.117728

Huimin Yuan, Hai-juan Li, Li-juan Wang, Chun-yang Zhang

{"title":"Tumor microenvironment-responsive dynamic self-assembly of DNAzyme nanowires for high-contrast imaging of multiple messenger RNAs and chemotherapy","authors":"Huimin Yuan, Hai-juan Li, Li-juan Wang, Chun-yang Zhang","doi":"10.1016/j.bios.2025.117728","DOIUrl":"10.1016/j.bios.2025.117728","url":null,"abstract":"<div><div>Messenger RNAs (mRNAs) are a category of protein-coding RNA, and their dysregulation is closely implicated in diverse cancers. Static DNA nanodevices have been engineered for the imaging and therapy, but they rely on the pre-assembly of DNA components and are limited by the low imaging contrast. Herein, we demonstrate the tumor microenvironment-responsive dynamic self-assembly of DNAzyme nanowires for high-contrast imaging of multiple mRNAs and chemotherapy. We design a cruciform DNA module (C-model) as a basic structural unit of DNAzyme nanowire, which consists of two DNAzyme strands and two substrate strands with the chemotherapeutic drug doxorubicin (DOX) being inserted into the C-models by inlaying between guanine and cytosine. Once approaching tumor cells, C-models with two split i-motif spontaneously assemble into a DNAzyme nanowire through protonation-driven Hoogsteen interaction in response to extracellular acidic microenvironments. Upon the internalization, intracellular TK1 mRNA and GalNAc-T mRNA hybridize with the overhangs of DNAzyme strands and substrate strands to form a stable DNAzyme/substrate/target three-way junction, inducing the cleavage of substrates by DNAzymes and the recovery of Cy5/Texas Red fluorescence. AS1411 aptamer-functionalized DOX-nanowires can selectively endocytose into cancer cells via AS1411-mediated recognition of nucleolin overexpressed on the cancer cell surface. Notably, the dynamic self-assembly of DNAzyme nanowires is characterized by efficient intracellular delivery and intrinsic resistance to nucleases, facilitating the high-contrast imaging of mRNAs. This method can accurately measure TK1 mRNA and GalNAc-T mRNA in living cells and discriminate multiple mRNAs in breast cancer tissues and their normal counterparts, providing a powerful platform for clinical diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117728"},"PeriodicalIF":10.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a label-free Ca2+ image sensor to visualize extracellular Ca2+ dynamics in the biological samples 开发无标签的Ca2+图像传感器，以可视化生物样品中的细胞外Ca2+动态

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-27 DOI: 10.1016/j.bios.2025.117729

Bijay Parajuli , Hideo Doi , Eiji Shigetomi , Hideaki Suzuki , Kozo Saito , Kazuaki Sawada , Schuichi Koizumi

{"title":"Development of a label-free Ca2+ image sensor to visualize extracellular Ca2+ dynamics in the biological samples","authors":"Bijay Parajuli , Hideo Doi , Eiji Shigetomi , Hideaki Suzuki , Kozo Saito , Kazuaki Sawada , Schuichi Koizumi","doi":"10.1016/j.bios.2025.117729","DOIUrl":"10.1016/j.bios.2025.117729","url":null,"abstract":"<div><div>Calcium ion (Ca<sup>2+</sup>) regulates various biological functions. Most studies, however, have focused on intracellular Ca<sup>2+</sup> (Ca<sup>2+</sup>i) and its role in cellular responses. Extracellular Ca<sup>2+</sup> ion (Ca<sup>2+</sup>o) also plays a crucial role in physiology serving as a key ion pool for entry into cells through various plasma membrane channels. Changes in Ca<sup>2+</sup>o occur under both physiological and pathological conditions and can influence biological responses. Despite its importance, Ca<sup>2+</sup>o has received little attention due to the lack of easy-to-use imaging devices. Here, we show the development of a label-free Ca<sup>2+</sup> image sensor (CIS) for monitoring Ca<sup>2+</sup>o. The CIS was developed by fabricating a charge transfer type CMOS image sensor with Ca<sup>2+</sup>-selective membrane and is highly selective for Ca<sup>2+</sup>o without detecting other ions. We used CIS to monitor glutamate-induced Ca<sup>2+</sup>o changes in the whole hippocampal slices. CIS detected different spatiotemporal patterns of Ca<sup>2+</sup>o decrease across the hippocampal slice, with the largest decrease at the CA1 pyramidal cell layer. This Ca<sup>2+</sup>o decrease was inhibited by the NMDA receptor antagonist but not by AMPA receptor antagonist and was mimicked by NMDA suggesting that Ca<sup>2+</sup> entry into neurons through NMDA receptors decreases Ca<sup>2+</sup>o. These results show that the CIS is a valuable tool for analyzing spatiotemporal dynamics of Ca<sup>2+</sup>o in normal physiology and pathological states and has tremendous potential for elucidating the biological function of Ca<sup>2+</sup>o.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117729"},"PeriodicalIF":10.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible microfluidics-integrated electrochemical system for detection of tumor necrosis factor-alpha under continuous flow of sweat 柔性微流体集成电化学系统在连续汗液下检测肿瘤坏死因子

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-26 DOI: 10.1016/j.bios.2025.117734

M. Ploner , B. Shkodra , L. Franchin , A. Altana , M. Petrelli , M.A. Costa Angeli , G. Ciccone , T. Antrack , L. Vanzetti , R.R. Nair , R. Canteri , S. Bonaldo , A. Paccagnella , H. Kleemann , D. Resnati , P. Lugli , A. Erten , L. Petti

{"title":"Flexible microfluidics-integrated electrochemical system for detection of tumor necrosis factor-alpha under continuous flow of sweat","authors":"M. Ploner , B. Shkodra , L. Franchin , A. Altana , M. Petrelli , M.A. Costa Angeli , G. Ciccone , T. Antrack , L. Vanzetti , R.R. Nair , R. Canteri , S. Bonaldo , A. Paccagnella , H. Kleemann , D. Resnati , P. Lugli , A. Erten , L. Petti","doi":"10.1016/j.bios.2025.117734","DOIUrl":"10.1016/j.bios.2025.117734","url":null,"abstract":"<div><div>Cytokines play a vital role in immune system signaling, making their detection crucial for continuous health monitoring. Among the various cytokines, tumor necrosis factor-alpha (TNF-<em>α</em>) stands out as a key regulator of the immune response. Notably, TNF-<em>α</em> can be detected in sweat at concentrations as low as pg/mL, with levels strongly correlated with those in blood. Despite its importance, sensitive, wearable, and continuous monitoring of TNF-<em>α</em> in sweat remains limited. To address this gap, this study presents a flexible electrochemical sensor integrated into a microfluidic system for the sensitive and selective detection of TNF-<em>α</em> under continuous sweat flow. First, we present the fabrication of two distinct, miniaturized designs of flexible screen-printed carbon three-electrode platforms, which are subsequently biofunctionalized with gold nanoparticles (AuNPs) coated with TNF-<em>α</em>-specific thiolated aptamers. Next, we compare the two geometrically distinct AuNP-aptamer-functionalized sensors, utilizing experimental and novel simulation-based characterization techniques. Finally, the sensors are integrated into a custom-built microfluidic system enabling the detection of TNF-<em>α</em> ranging from 0.2 to 1000 pg/mL under constant artificial sweat flow conditions, exhibiting high selectivity with negligible responses to non-specific analytes. These findings highlight the feasibility of integrating wearable cytokine sensors for detecting TNF-<em>α</em> under continuous sweat flow conditions, achieving clinically relevant sensitivity within the pg/mL range.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117734"},"PeriodicalIF":10.7,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small-molecule fluorescence probes for biomarkers of Alzheimer's disease 小分子荧光探针用于阿尔茨海默病的生物标志物

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-25 DOI: 10.1016/j.bios.2025.117722

Juanxia Wang , Xu Shao , Chao Wang , Shengqi Wu , Mengyao Bai , Pengtao Zhang , Selene Tang , Nan Zhang , Xin Wang , Zhen Yang , Xiaoling Hu , Ping Guan , Zhaoyuan Lyu , Shichao Ding

{"title":"Small-molecule fluorescence probes for biomarkers of Alzheimer's disease","authors":"Juanxia Wang , Xu Shao , Chao Wang , Shengqi Wu , Mengyao Bai , Pengtao Zhang , Selene Tang , Nan Zhang , Xin Wang , Zhen Yang , Xiaoling Hu , Ping Guan , Zhaoyuan Lyu , Shichao Ding","doi":"10.1016/j.bios.2025.117722","DOIUrl":"10.1016/j.bios.2025.117722","url":null,"abstract":"<div><div>Alzheimer's disease (AD) is the prevalent neurodegenerative disorder characterized by the accumulation of aberrant proteins that obstruct brain cells and neurons. Early detection of Alzheimer's disease-related biomarkers is very critical for effective treatment of AD. However, the current probes for early clinical diagnosis of AD are invasive and costly, susceptible to interference by biological background fluorescence, and lack sufficient penetration capabilities and challenges associated with imaging <em>in vivo</em>. Therefore, it is very important to develop small-molecular fluorescent probes with biocompatibility, strong penetration ability of the blood-brain barrier (BBB), customizable physical and chemical properties, and real-time non-destructive in situ monitoring. This review provides a comprehensive overview of protein aggregates, reactive oxygen species, metal ions, gas markers, cholinesterase, cysteine, and other recently discovered biomarkers, concentrating on the mechanism of targeted diagnosis and the relationship between probe structure and characteristics. In addition, the review also summaries recent advances in machine learning and artificial intelligence for AD field. Furthermore, the challenges and prospects of small-molecule fluorescent probes for AD diagnosis and clinical application are also summarized. This review aims to provide a foundation for researchers in this field, stimulate innovative molecular design strategies, expedite the clinical evaluation of AD probes, and ultimately achieving accurate early detection of AD.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117722"},"PeriodicalIF":10.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144517295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and application of a highly specific whole-cell biosensor for supersulfide detection in environmental samples 用于环境样品超硫化物检测的高特异性全细胞生物传感器的开发与应用

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-25 DOI: 10.1016/j.bios.2025.117731

Fan Liu , Min Wang , Huaiwei Liu , Xianzhe Gong , Ning Du , Qilong Qin , Yun Tian , Luying Xun , Yongzhen Xia

{"title":"Development and application of a highly specific whole-cell biosensor for supersulfide detection in environmental samples","authors":"Fan Liu , Min Wang , Huaiwei Liu , Xianzhe Gong , Ning Du , Qilong Qin , Yun Tian , Luying Xun , Yongzhen Xia","doi":"10.1016/j.bios.2025.117731","DOIUrl":"10.1016/j.bios.2025.117731","url":null,"abstract":"<div><div>Supersulfides are a class of sulfur compounds in either zero-valent or monovalent negative states, such as elemental sulfur, inorganic polysulfides, and organic polysulfides, and they play crucial roles in biological systems, environmental sulfur cycling, and Li-S batteries. However, accurate quantification of these compounds remains challenging due to their reactive nature and structural complexity. Here, we report the first whole-cell biosensor for the specific detection of supersulfides using the supersulfide-sensing repressor BigR of <em>Agrobacterium tumefaciens</em> (AtBigR). This novel biosensor system shows exceptional selectivity for supersulfides with negligible response to H<sub>2</sub>S, thiosulfate, and glutathione. Through systematic optimization of the AtBigR-based whole-cell biosensor (AtBigR-WCB), we developed a biosensor with negligible background leakage and high sensitivity, capable of detecting supersulfides at micromolar levels. We successfully applied AtBigR-WCB to quantify supersulfide contents in marine sediments from diverse seafloor topographies and lithium polysulfides in lithium-sulfur battery electrolytes. Widespread supersulfide accumulation was detected in deeper layers of marine sediments across various environments. Additionally, the optimized AtBigR regulatory system was a novel, low-leakage regulating system, which offers a new type of gene-regulating system with potential applications in synthetic biology. Our work establishes a new platform for quantifying supersulfides, which is helpful to further our understanding of supersulfides in biology, environments, and lithium sulfur batteries.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117731"},"PeriodicalIF":10.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144517447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metasurface-enhanced terahertz imaging for glioblastoma in orthotopic xenograft mouse model combined with neural network decision making 结合神经网络决策的超表面增强太赫兹成像在原位异种移植小鼠胶质母细胞瘤模型中的应用

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-25 DOI: 10.1016/j.bios.2025.117715

Yeeun Roh , Kyu-hyeon Kim , Geon Lee , Jinwoo Lee , Taeyeon Kim , Beomju Shin , Dong Min Kang , Yun Kyung Kim , Minah Seo

{"title":"Metasurface-enhanced terahertz imaging for glioblastoma in orthotopic xenograft mouse model combined with neural network decision making","authors":"Yeeun Roh , Kyu-hyeon Kim , Geon Lee , Jinwoo Lee , Taeyeon Kim , Beomju Shin , Dong Min Kang , Yun Kyung Kim , Minah Seo","doi":"10.1016/j.bios.2025.117715","DOIUrl":"10.1016/j.bios.2025.117715","url":null,"abstract":"<div><div>Terahertz (THz) optical sensing and imaging offer significant potential in a range of biological and medical applications owing to their low-energy, non-ionizing nature, and ultra-broadband spectral information, which includes numerous molecular fingerprints. However, conventional THz imaging suffers from limited contrast and low absorption cross-section in biological tissues. Recent advances in terahertz sensing platforms, facilitated by various metasurfaces, have addressed these limitations by enhancing the sensitivity and selectivity of optical detection and imaging. This study presents an advanced label-free terahertz imaging technique that leverages a metasurface to enhance image contrast. We applied this method to image glioblastoma model mouse brain tissues. To identify cancerous regions clearly, the complex refractive indices across the brain tissues were determined using a finite element method simulation. Furthermore, the strong resonance features of the metasurface facilitate correlation-based learning in neural networks. We employed a convolutional neural network to segment cancer boundaries using the metasurface-enhanced imaging data. Glioblastoma regions were identified with an accuracy of over 99 %, by using fluorescence-labeled images as the training data for the neural networks. This study highlights the critical role of metasurfaces in fundamentally enhancing terahertz wave-matter interactions and how integration with neural networks enables highly sensitive cancer detection. This paves the way for the clinical applications of terahertz imaging technologies in medical diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117715"},"PeriodicalIF":10.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transferring the released component of Z-scheme heterojunction onto opposite photoelectrode: A dual-electrode-cooperating signal amplification strategy in photo fuel cell for self-powered biosensing 将z型异质结的释放组分转移到对侧光电极上：用于自供电生物传感的光燃料电池双电极合作信号放大策略

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-25 DOI: 10.1016/j.bios.2025.117732

Yang Lei , Ziyi Zhang , Cui Wang , Rong-Bin Song , Zhaohui Li

{"title":"Transferring the released component of Z-scheme heterojunction onto opposite photoelectrode: A dual-electrode-cooperating signal amplification strategy in photo fuel cell for self-powered biosensing","authors":"Yang Lei , Ziyi Zhang , Cui Wang , Rong-Bin Song , Zhaohui Li","doi":"10.1016/j.bios.2025.117732","DOIUrl":"10.1016/j.bios.2025.117732","url":null,"abstract":"<div><div>Exploration of signal amplification strategy is an important link during the development of self-powered photoelectrochemical biosensors (SPECs). Herein, we develop a dual-electrode-cooperating signal amplification mode by transferring the released component of Z-scheme heterojunction onto opposite photoelectrode, and integrate it with DNA entropy-driven amplification design for ultrasensitive SPECs bioanalysis. Specifically, microRNA-155, the model analyte triggers the entropy-driven DNA circuit to release large amounts of output DNAs, which can competitively hybridize with the partial complementary DNA double strand between TiO<sub>2</sub> nanospheres and Sb<sub>2</sub>S<sub>3</sub>/Au photoanode for initiating the release of TiO<sub>2</sub> nanospheres. In this case, the formed Z-scheme heterojunction on photoanode will suffer destruction, generating a positive shift of photoanode potential and thus a decrease in the open circuit voltage (E<sup>OCV</sup>) of SPECs. Meanwhile, the DNA on the surfaces of these liberated TiO<sub>2</sub> nanospheres can further hybridize with the hairpin DNA anchored on CuO/Cu<sub>2</sub>O photocathode, leading to the formation of type-II heterojunction and the negative shift of photocathode potential. Therefore, an additional decrease in the E<sup>OCV</sup> of SPECs can be realized for cascading signal amplification. This work adds a new member to the family of signal amplification strategies from dual-electrode-cooperating perspective, which will attract more attentions in the field of SPECs and even other fuel cells-based biosensor.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117732"},"PeriodicalIF":10.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ellagic acid-imbedded MoO3 nanowires architecture: External mediator-less electrochemical immunoprobing of inflammatory bowel disease marker 鞣花酸嵌入MoO3纳米线结构：炎症性肠病标志物的无外源性介质电化学免疫探测

IF 10.7 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-06-24 DOI: 10.1016/j.bios.2025.117719

Gaurav Rajkumar Pandey , Somasundaram Arumugam , Jayaprakash Thulukanam , Leela Kakithakara Vajravelu , Murugan Veerapandian

{"title":"Ellagic acid-imbedded MoO3 nanowires architecture: External mediator-less electrochemical immunoprobing of inflammatory bowel disease marker","authors":"Gaurav Rajkumar Pandey , Somasundaram Arumugam , Jayaprakash Thulukanam , Leela Kakithakara Vajravelu , Murugan Veerapandian","doi":"10.1016/j.bios.2025.117719","DOIUrl":"10.1016/j.bios.2025.117719","url":null,"abstract":"<div><div>Oxidative stress biomarkers such as 8-iso-prostaglandin F<sub>2α</sub> (8-iso-PGF2α) dictate a central dogma of clinically prevalent (non) communicable diseases such as inflammatory bowel disease (IBD) but are less explored owing to the complexity, sensitivity, and selectivity of conventional detecting methods. Thus, there is an urgent need for a rapid, simple, and reliable method for point-of-care (POC) detection of 8-iso-PGF2α. Herein, linker chemistry mediated ellagic acid imbedded MoO<sub>3</sub> NWs architecture is employed as an external mediator-free transducer to develop an 8-iso-PGF2α biosensor. The optimized biosensor provided robust and discriminative analytical performance for a wide range (0.5–5000 pg/mL) and LOD∼0.28 pg/mL with good reproducibility (RSD 2.0 %). The 8-iso-PGF2α biosensor's practicality towards clinical sample detection collected from IBD patients is demonstrated and cross-validated against the ELISA using the Blend-Altman technique, establishing a strong correlation, negligible bias and coefficient of variance (<span><math><mrow><mover><mi>X</mi><mo>˜</mo></mover></mrow></math></span> = 6.88 %) with the added advantage of user-friendliness and fast analytical response (∼60 s). Thus, the proposed biosensor is a reliable POC screening tool capable of predicting disease onset, monitoring progression, and assessing treatment efficacy in large longitudinal cohorts and clinical landscapes.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117719"},"PeriodicalIF":10.7,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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