Biosensors and Bioelectronics最新文献_第10页

A synthetic gene circuit to convert biochemical signals to electrical signal in engineered sensory bacteria for electrochemical biosensing 一种将生物化学信号转化为电信号的合成基因电路，用于电化学生物传感。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-04-15 Epub Date: 2026-01-12 DOI: 10.1016/j.bios.2026.118391

Xinlu Wu , Tianxiang Wei , Jingwen Zhang , Jinming Zhao , Kai Xu , Haijia Yu , Tao Gao

{"title":"A synthetic gene circuit to convert biochemical signals to electrical signal in engineered sensory bacteria for electrochemical biosensing","authors":"Xinlu Wu , Tianxiang Wei , Jingwen Zhang , Jinming Zhao , Kai Xu , Haijia Yu , Tao Gao","doi":"10.1016/j.bios.2026.118391","DOIUrl":"10.1016/j.bios.2026.118391","url":null,"abstract":"<div><div>The intracellular gene circuit to transform biochemical signals to an electrical signal is the key for biosensor development to gap the information mismatch at the bio-electric interface, but a qualified gene circuit is difficult to design. In this study, we have shown the construction of an integrated synthetic gene circuit including the nitric oxide (NO)-responsive module and the phenylenediamine-1-carboxylic acid (PCA) synthesis module, to (1) equip a less renounced electroactive bacterium (e.g. <em>Escherichia coli</em>) with indirect electron transfer (IET) pathway to enhance electrical signal output, as well as (2) couple the IET gene circuit with the responsive gene circuit to sense small signal molecule, NO. The subsequent oxidation of PCA can be electrochemically quantified by the microelectrode, thereby establishing a signaling pathway from intracellular message to redox mediator, and finally to electrical signal output. In this way, the constructed microbial electrochemical biosensor for intracellular <em>in situ</em> NO analysis at the single-cell level owns high sensitivity, a wide linear detection range (100–2500 nM), and excellent selectivity. Therefore, this work shows an example for developing next-generation electroactive microorganisms (EAMs)-based electrochemical biosensors through synthetic biology tools with tailored and intelligent functionalities.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118391"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145996804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A structure-optimized BRET biosensor for rapid, dilution-free quantification of high-strength nitrate in wastewater 一种结构优化的BRET生物传感器，用于废水中高强度硝酸盐的快速、无稀释定量。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-04-15 Epub Date: 2026-01-22 DOI: 10.1016/j.bios.2026.118434

Kanglin Zhao , Hao Bai , Ziqi Sha , Xinpeng Hu , Yan Zhang , Xuxiang Zhang

{"title":"A structure-optimized BRET biosensor for rapid, dilution-free quantification of high-strength nitrate in wastewater","authors":"Kanglin Zhao , Hao Bai , Ziqi Sha , Xinpeng Hu , Yan Zhang , Xuxiang Zhang","doi":"10.1016/j.bios.2026.118434","DOIUrl":"10.1016/j.bios.2026.118434","url":null,"abstract":"<div><div>A bioluminescence resonance energy transfer (BRET) biosensor was developed for rapid, dilution-free quantification of high-strength nitrate in wastewater. The sensor integrates the <em>Escherichia coli</em> nitrate receptor NarX with a high-brightness NLuc/VenusΔC10 reported pair and was optimized by computationally guided mutagenesis (NarXV138W), structure-informed terminal truncation, and assay parameter tuning. The optimized construct, VenusΔC12–N5NarXV138WΔC3–ΔN4NLuc, enabled accurate quantification from 0.0781 to 2.5710 g/L (as N) with a four-parameter logistic fit (R<sup>2</sup> = 0.999) and a ΔBRET of 0.28 after ∼500 s equilibration. Measurements proceeded under mild aqueous conditions and showed strong agreement with UV–visible spectrophotometry in both simulated and real water samples, eliminating the need for extensive dilution. Circular dichroism and mass spectrometry analyses revealed nitrate-dependent structural changes that are supportive of ligand-induced signaling mechanisms. To our knowledge, this represents the first BRET-based biosensing platform tailored to industrial-range nitrate detection, offering a simple, ratiometric, and reagent-efficient approach suitable for <em>in situ</em> monitoring of industrial and municipal effluents.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118434"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light-addressable sandwich photoelectrochemical immunosensor array and lateral flow immunoassays with self-calibration using quantum dots-sensitized and porphyrin-engineered MOFs for accurate detection of amyloid β-proteins 使用量子点敏化和卟啉工程mof的光寻址三明治光电化学免疫传感器阵列和具有自校准的横向流动免疫分析，用于淀粉样蛋白β-蛋白的准确检测

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-04-15 Epub Date: 2026-01-19 DOI: 10.1016/j.bios.2026.118424

Louqun Wang , Jingge Niu , Xiaoli Chen , Yun Zhu , Xiaohong Hou

{"title":"Light-addressable sandwich photoelectrochemical immunosensor array and lateral flow immunoassays with self-calibration using quantum dots-sensitized and porphyrin-engineered MOFs for accurate detection of amyloid β-proteins","authors":"Louqun Wang , Jingge Niu , Xiaoli Chen , Yun Zhu , Xiaohong Hou","doi":"10.1016/j.bios.2026.118424","DOIUrl":"10.1016/j.bios.2026.118424","url":null,"abstract":"<div><div>Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the accumulation of amyloid-beta (Aβ) plaques, making Aβ a crucial biomarker for early diagnosis. In this study, we developed a novel light-addressable sandwich photoelectrochemical (PEC) immunosensor array for the detection of Aβ42, aiming to enhance diagnostic methods for AD. The array utilized cadmium telluride quantum dot-sensitized UiO-66 (U@C) as the sensing matrix and PCN-224@ZnIn<sub>2</sub>S<sub>4</sub> nanoflower heterojunctions (P@Z) as immunoprobes, enabling multiplexed detection through laser pen activation on a single FTO electrode with a self-calibration method to minimize background noise and baseline drift. The assay required 60 min for incubation steps before detection; however, with 11 simultaneous detection points, it significantly reduced batch processing time and enhanced practicality. For point-of-care applications, we also developed a PEC-lateral flow immunoassay (PEC-LFIA) test strip that allowed for real-time, quantitative detection of Aβ42 with only 15 min of incubation required for testing. Both platforms effectively detected Aβ42 in PBS, artificial cerebrospinal fluid (CSF), and human plasma at concentrations ranging from fg/mL to ng/mL. Notably, sensitivity in human plasma was five times greater than that in artificial CSF. The PEC array achieved detection limits of 19.5 fg/mL in CSF and 3.1 fg/mL in plasma, while the PEC-LFIA strip demonstrated limits of 17.8 fg/mL in CSF and 3.1 fg/mL in human plasma. These advancements significantly reduced patient burden and brought us closer to utilizing a single drop of blood for monitoring brain aging.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118424"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated sample-in-result-out microfluidic biosensor for rapid ultrasensitive detection of Salmonella typhimurium using electromagnetically actuated aptamer-conjugated magnetic bead chains 采用电磁驱动适配体共轭磁珠链的集成样品-结果输出微流控生物传感器快速超灵敏检测鼠伤寒沙门菌

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-04-15 Epub Date: 2026-01-19 DOI: 10.1016/j.bios.2026.118422

Lemin Yu , Jingyi Wang , Shoukun Lin , Wenzhe Lu , Quan Li , Yubo Shi , Jingfu Wang

{"title":"Integrated sample-in-result-out microfluidic biosensor for rapid ultrasensitive detection of Salmonella typhimurium using electromagnetically actuated aptamer-conjugated magnetic bead chains","authors":"Lemin Yu , Jingyi Wang , Shoukun Lin , Wenzhe Lu , Quan Li , Yubo Shi , Jingfu Wang","doi":"10.1016/j.bios.2026.118422","DOIUrl":"10.1016/j.bios.2026.118422","url":null,"abstract":"<div><div><em>Salmonella typhimurium</em> (<em>S</em>. <em>typhimurium</em>)-induced contamination is emerging as a severe threat to food safety. Therefore, rapid and sensitive detection of <em>S. typhimurium</em> is urgently required. Conventional detection methodologies exhibit persistent limitations. The combination of microfluidic chips with magnetic beads (MBs) for isolating bacteria such as <em>S. typhimurium</em> has emerged as a prominent research focus. However, flexible and tunable magnetic field control methods for optimizing MB chain formation and enhancing the capture efficiency of the target bacteria are lacking. We developed a microfluidic fluorescent biosensor to achieve an efficient on-site detection of <em>S. typhimurium</em> using magnetic bead (MB) chains for continuous-flow electromagnetic separation, a dual-aptamer recognition method for specifically labeling <em>S. typhimurium</em>, and a customized smartphone-based real-time fluorescence system for quantitative detection. Aptamer-conjugated MBs in the separation channel were distributed into high-density bead chains in a high-intensity gradient magnetic field generated by an electromagnetic actuation module. The samples continuously flew through the separation channel, in which the target bacteria were specifically captured by aptamer-conjugated MBs. Aptamer-functionalized fluorescent labels were passed through the separation channel to form bacteria–MB–fluorescent label complexes for quantitative detection. Under optimal conditions, the microfluidic biosensor completed sample-in-result-out detection within 30 min, with a detection limit of 2 colony-forming unit/mL for <em>S</em>. <em>typhimurium</em>. This biosensor can be potentially applied for detecting food pathogens, making it suitable for supervising food production and monitoring catering hygiene.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118422"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of glycosylation-related changes in migratory and mechanical properties of bladder cancer cells 膀胱癌细胞迁移和机械特性中糖基化相关变化的鉴定

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-04-15 Epub Date: 2026-01-21 DOI: 10.1016/j.bios.2026.118410

Marcin Luty , Renata Szydlak , Joanna Pabijan , Ingrid H. Øvreeide , Victorien E. Prot , Joanna Zemła , Bjørn T. Stokke , Małgorzata Lekka

{"title":"Identification of glycosylation-related changes in migratory and mechanical properties of bladder cancer cells","authors":"Marcin Luty , Renata Szydlak , Joanna Pabijan , Ingrid H. Øvreeide , Victorien E. Prot , Joanna Zemła , Bjørn T. Stokke , Małgorzata Lekka","doi":"10.1016/j.bios.2026.118410","DOIUrl":"10.1016/j.bios.2026.118410","url":null,"abstract":"<div><div>Glycosylation, a fundamental posttranslational modification, is crucial in cancer progression. Aberrant glycosylation patterns in tumor cells alter their interactions with the extracellular matrix (ECM), facilitating invasion and metastasis. Lectins are carbohydrate-binding proteins that selectively recognize these modifications, making them valuable tools for cancer detection. However, their impact on cancer cell behavior remains insufficiently explored.</div><div>In this study, we examined the response of bladder cancers (non-malignant HCV29, non-invasive HT1376, and invasive T24 cancer cells) to surfaces coated with the lectins (<em>Dolichos</em> <em>b</em><em>iflorus</em> agglutinin (DBA), <em>Lens</em> <em>c</em><em>ulinaris</em> agglutinin (LCA), <em>Phaseolus vulgaris</em> leucoagglutinin (PHA-L), and <em>Wheat</em> <em>g</em><em>erm</em> agglutinin (WGA)), using atomic force microscopy (AFM), cell imaging, and proliferation and migration assays. Our findings indicate that lectin coatings significantly influenced cancer cell proliferation and migration in a lectin-dependent manner. Notably, WGA induced the most pronounced effects, accelerating proliferation while simultaneously reducing migration, particularly in the invasive T24 cell line. Similarly, PHA-L increased proliferation. These results highlight the dual role of lectins as diagnostic markers and potential modulators of cancer cell behavior. While lectin-based biosensors offer promising tools for cancer detection, their influence on cell properties must be carefully considered to ensure reliable diagnostics. This study underscores the need for further research into the functional consequences of lectin-glycan interactions in cancer detection. Lectins, being beneficial for biosensing, may modulate cell behavior (as our results show), which potentially interferes with diagnostic capabilities.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118410"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-emission near-infrared fluorogenic probe for sensing Catechol-O- methyltransferase activity from in vitro to in vivo 一种双发射近红外荧光探针，用于检测从体外到体内的儿茶酚- o -甲基转移酶活性。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-04-15 Epub Date: 2026-01-15 DOI: 10.1016/j.bios.2026.118409

Fang-Yuan Wang , Lai Wei , Pu Wang , Wen-Chao Chen , Zu-Jia Chen , Zi-Qiong Zhou , Guang-Hao Zhu , Qing Gong , Lu-Ping Qin (∗∗∗∗) , Ling Yang (∗∗∗) , Guang-Bo Ge (∗∗) , Ping Wang (∗)

{"title":"A dual-emission near-infrared fluorogenic probe for sensing Catechol-O- methyltransferase activity from in vitro to in vivo","authors":"Fang-Yuan Wang , Lai Wei , Pu Wang , Wen-Chao Chen , Zu-Jia Chen , Zi-Qiong Zhou , Guang-Hao Zhu , Qing Gong , Lu-Ping Qin (∗∗∗∗) , Ling Yang (∗∗∗) , Guang-Bo Ge (∗∗) , Ping Wang (∗)","doi":"10.1016/j.bios.2026.118409","DOIUrl":"10.1016/j.bios.2026.118409","url":null,"abstract":"<div><div>Human catechol-<em>O</em>-methyltransferase (COMT, EC 2.1.1.6) plays a key role in neurotransmitter signaling and metabolism in the central nervous system. Optical sensing of COMT activity provides a powerful approach to study its biological functions, however, existing fluorescent probes are limited by short emission wavelengths and low sensitivity. Herein, we present <strong>TCFC</strong>, the first dual-emission near-infrared (NIR) fluorogenic substrate for COMT, rationally designed via a structure-modulated strategy. Under physiological conditions, <strong>TCFC</strong> was rapidly <em>O</em>-methylated by COMT to yield product <strong>8-MTCFC</strong>, which exhibited strong fluorescence at 620 nm and 695 nm upon excitation at 470 nm and 610 nm, respectively. The dual-emission property of <strong>TCFC</strong> enhanced detection sensitivity and versatility, enabling accurate assessment of COMT activity across individuals and species while supporting high-throughput screening of potential COMT inhibitors. Moreover, the NIR emission of <strong>TCFC</strong> allowed <em>in situ</em> visualization of COMT activity in living cells and various brain regions of rats with high spatiotemporal resolution, and revealed a reduction of COMT activity in the hippocampal region of a mouse model of Alzheimer's disease, offering insights into its role in neurodegeneration. Collectively, <strong>TCFC</strong> proved to be a powerful NIR fluorogenic substrate for monitoring COMT activity in complex biological systems, enabling mechanistic studies and high-throughput screening of potential inhibitors.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118409"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laser fabrication of flexible electrodes for bioelectronics 生物电子学用柔性电极的激光制造

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-04-15 Epub Date: 2026-01-09 DOI: 10.1016/j.bios.2026.118386

Lican Zheng , Yuyao Lu , Honghao Lyu , Tianyu Li , Songya Cui , Yuhong Xu , Zimo Cai , Yuyu Hou , Yibo Li , Qianqian Yang , Zhiqiu Ye , Geng Yang , Kaichen Xu

{"title":"Laser fabrication of flexible electrodes for bioelectronics","authors":"Lican Zheng , Yuyao Lu , Honghao Lyu , Tianyu Li , Songya Cui , Yuhong Xu , Zimo Cai , Yuyu Hou , Yibo Li , Qianqian Yang , Zhiqiu Ye , Geng Yang , Kaichen Xu","doi":"10.1016/j.bios.2026.118386","DOIUrl":"10.1016/j.bios.2026.118386","url":null,"abstract":"<div><div>Bioelectronics lies at the intersection of electronics and biology, enabling real-time signal exchange between living systems and machines. As next-generation applications such as wearable diagnostics, brain-computer interfaces, and closed-loop therapeutic systems desire for soft, miniaturized, and biocompatible platforms, the role of bioelectrodes becomes even more critical. Direct laser writing (DLW) has emerged as a powerful microscale fabrication approach, capable of directly patterning functional electrodes with high spatial resolution on diverse materials. In addition, DLW uniquely offers localized material processing and property modulation, enabling controlled synthesis, phase transition, and surface functionalization. This review presents a comprehensive overview of the underlying mechanisms and advanced material systems that enable DLW. We highlight how DLW enables structural design that impart stretchability and tissue conformity, and how such electrodes are integrated into wearable and implantable bioelectronic systems. Finally, we discuss key challenges and future opportunities for DLW-based bioelectrodes, which are poised to become foundational components of intelligent and adaptive biomedical interfaces.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118386"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145963072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplexed and continuous single-molecule biosensing by spectral demixing of plasmon-enhanced fluorescence 等离子体增强荧光光谱脱混的多路和连续单分子生物传感。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-04-15 Epub Date: 2026-01-10 DOI: 10.1016/j.bios.2026.118384

Sjoerd W. Nooteboom , Vincenzo Lamberti , Tim Schellekens , Peter Zijlstra

{"title":"Multiplexed and continuous single-molecule biosensing by spectral demixing of plasmon-enhanced fluorescence","authors":"Sjoerd W. Nooteboom , Vincenzo Lamberti , Tim Schellekens , Peter Zijlstra","doi":"10.1016/j.bios.2026.118384","DOIUrl":"10.1016/j.bios.2026.118384","url":null,"abstract":"<div><div>Continuous monitoring of the concentration of biomarkers is a crucial step forward to capture time-dependent changes in early stage diagnostics. A novel class of continuous monitoring sensors relies on low-affinity interactions that are probed at the single-molecule level. Nevertheless, integration of multixplexing capabilities remains a tremendous challenge, despite its promise to provide more accurate diagnostics that will allow for early intervention. Here we demonstrate a multiplexed biosensor capable of continuous monitoring using single-molecule plasmon-enhanced fluorescence. Unfortunately, plasmon-enhancement of fluorescence not only leads to increased signal brightness but also causes strong reshaping of the dye’s emission spectrum. This has prohibited multiplexing that is most commonly implemented using multiple fluorophores of different colors. Here we overcome this barrier by experimentally quantifying the spectral reshaping as a function of the plasmon wavelength and linewidth. Using this knowledge we present a new spectral demixing approach that recovers the intrinsic dye spectrum from the spectrally distorted plasmon-enhanced spectrum. This enables the recovery of the intrinsic emission peak with a 2-fold improved precision despite the spectral distortion. We then demonstrate the use of spectral demixing for single-molecule biosensing where we show the multiplexed and continuous monitoring of two nucleic acids simultaneously. The approach enables the use of a single laser line for multicolor sensing while providing a high signal-to-noise ratio to enable cost-effective and multiplexed monitoring of biomarkers.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118384"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasensitive extracellular vesicles-associated amyloid-β1-42 oligomers analytical platform for early diagnosis of Alzheimer's disease 超灵敏细胞外小泡相关淀粉样蛋白-β1-42寡聚物早期诊断阿尔茨海默病分析平台

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-04-15 Epub Date: 2026-01-22 DOI: 10.1016/j.bios.2026.118430

Peijuan Xie , Xueli Wang , Haiyan Wang , Huanxin Xue , Sijia He , Longfei Li , Man Shing Wong , Xiaoqiang Qiao

{"title":"Ultrasensitive extracellular vesicles-associated amyloid-β1-42 oligomers analytical platform for early diagnosis of Alzheimer's disease","authors":"Peijuan Xie , Xueli Wang , Haiyan Wang , Huanxin Xue , Sijia He , Longfei Li , Man Shing Wong , Xiaoqiang Qiao","doi":"10.1016/j.bios.2026.118430","DOIUrl":"10.1016/j.bios.2026.118430","url":null,"abstract":"<div><div>Extracellular vesicles (EVs) are increasingly recognized as promising biomarkers for central nervous system diseases, including Alzheimer's disease (AD). However, early and accurate AD diagnosis remains challenging due to the extremely low abundance of relevant biomarkers in body fluids. Here, we present an ultrasensitive and reliable platform for quantifying trace levels of EVs-associated Aβ<sub>1-42</sub> oligomers (EVs@Aβ<sub>1-42</sub>) in plasma. The method integrates UiO-66-B(OH)<sub>2</sub> nanomaterials for high-efficiency enrichment of EVs with the i-SQM “turn-on” fluorescent probe, which selectively responds to Aβ<sub>1-42</sub> oligomers. This combined strategy enables rapid quantification within minutes and provides a large fluorescence enhancement with excellent signal-to-noise ratio, achieving femtomolar-level sensitivity. In clinical samples, the platform successfully distinguishes AD patients from cognitively normal individuals with 100 % sensitivity and 96.3 % specificity. Overall, this EVs-based detection system offers a practical, low-cost, and non-invasive approach for plasma biomarker analysis and holds strong potential for early AD diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118430"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A microfluidic microspheres accumulation platform for direct visualization of urine albumin-to-creatinine ratio in patients with chronic kidney disease 用于慢性肾病患者尿白蛋白与肌酐比值直接可视化的微流控微球积累平台。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2026-04-15 Epub Date: 2026-01-13 DOI: 10.1016/j.bios.2026.118400

Chenyu Cui , Hogi Hartanto , Xinxue Yin , Jiaheng Li , Choi Wan Luk , Cheuk Chun Szeto , Ting-Hsuan Chen

{"title":"A microfluidic microspheres accumulation platform for direct visualization of urine albumin-to-creatinine ratio in patients with chronic kidney disease","authors":"Chenyu Cui , Hogi Hartanto , Xinxue Yin , Jiaheng Li , Choi Wan Luk , Cheuk Chun Szeto , Ting-Hsuan Chen","doi":"10.1016/j.bios.2026.118400","DOIUrl":"10.1016/j.bios.2026.118400","url":null,"abstract":"<div><div>The urine albumin-to-creatinine ratio (uACR) level is an essential biomarker for the early screening and monitoring of chronic kidney disease (CKD), particularly among patients with diabetic and hypertension. However, uACR requires quantitative assay, where the use of designated analyzers makes the assays difficult for large-scale screening. In this study, we developed a decentralized, instrument-free microfluidic microspheres accumulation platform (MMAP) that visualizes the uACR in patients with diabetic nephropathy. Albumin inhibits the binding between magnetic microspheres (MMSs) and polymer microspheres (PMSs) due to competitive immunoassay interactions, while creatinine is recognized by the anti-creatinine aptamer, which releases a DNA blocker strand to facilitate connections between MMSs and PMSs through DNA hybridization. The binding of MMSs and PMSs prevents PMSs from escaping magnetic attraction in the inlet, thereby reducing the free PMS accumulation length in the measurement zone of the microfluidic chip. The PMS accumulation length allows for the visual quantification of uACR with excellent selectivity, tolerance to varying pH levels, and high accuracy comparable to that of standard tests in the hospital. As a convenient, low-cost assessment tool that correctly classified all clinical samples in this pilot cohort to evaluate CKD stages from normal to end-stage, this device offers significant benefits for disease self-monitoring and community health services.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118400"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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