Biosensors and Bioelectronics最新文献

{"title":"“Sample-In, Result-Out” liquid biopsy chip based on immunomagnetic separation and CRISPR detection for multiplex analysis of exosomal microRNAs","authors":"Niancai Peng ,&nbsp;Xueqin Gao ,&nbsp;Zhang Yong ,&nbsp;Yunyun Zhang ,&nbsp;Xiaoniu Guo ,&nbsp;Qiaochu Wang ,&nbsp;Yong Wan ,&nbsp;Shuhao Zhao ,&nbsp;Tianyi Zhang ,&nbsp;Fei Hu","doi":"10.1016/j.bios.2025.117460","DOIUrl":"10.1016/j.bios.2025.117460","url":null,"abstract":"<div><div>Multiplex analysis of exosomal microRNAs (miRNAs) plays an important role in noninvasive early disease diagnosis. However, the complexity of the testing process has hindered its clinical application. Here, we proposed an integrated chip for the detection of eight exosomal miRNAs in serum which can achieve “sample in, result out” detection. We developed an immunomagnetic isolation system based on CD63 aptamers (IISA) for separation of serum exosomes. The system was combined with immiscible filtration assisted by surface tension (IFAST) to remove impurities. Bubble mixing was applied to ensure adequate binding or cleavage of exosomes to magnetic beads. CRISPR detection technology was utilized to allow for effective detection of seven hepatocellular carcinoma (HCC)-related miRNA targets. Based on the test of clinical samples, the chip can achieve 78% exosome capture efficiency and 55% recovery, and simultaneously detect eight targets within 1 h. This chip could be applied as a robust and cost-effective tool for cancer diagnosis and monitoring of cancer stages.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"280 ","pages":"Article 117460"},"PeriodicalIF":10.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ cleavage-based sortase A-mediated site-specific immobilization of beta2-adrenoceptor on gold surface for surface plasmon resonance measurement","authors":"Xue Zhao ,&nbsp;Yuanyuan Ou ,&nbsp;Ruoxue Bai ,&nbsp;Jiatai Yin ,&nbsp;Ge Wang ,&nbsp;Jing Wang ,&nbsp;Xinfeng Zhao ,&nbsp;Yinku Liang ,&nbsp;Qian Li","doi":"10.1016/j.bios.2025.117452","DOIUrl":"10.1016/j.bios.2025.117452","url":null,"abstract":"<div><div>Surface Plasmon Resonance (SPR) is a pivotal technique for measuring biomolecular interactions, with the sensor surface typically made of gold or silver and requiring proteins to be immobilized in a controlled manner. Traditional methods, such as random crosslinking via covalent amide bonds (EDC/NHS strategy), resulting in diverse protein orientations. Alternatively, site-specific immobilization strategies offer better orientation control, they are still challenged by the purification needs for protein of interests and steric hindrance produced by bulk protein tags. To address these issues, we proposed a novel protein immobilization strategy relying on <em>in situ</em> cleavage and Sortase A (SrtA) to immobilize functional protein on SPR sensor chips. This strategy involves the β₂-adrenoceptor (β₂AR) as a model, incorporating an endogenous protease recognition site (EPRS) as a linker to fuse SrtA with β₂AR, which contains an SrtA recognition sequence (LPXTG) at its C-terminal. When expressed in <em>Escherichia coli</em> (<em>E. coli</em>), the protease cleaves the EPRS, releasing SrtA and β₂AR. When the lysate is mixed with an oligo-Gly or oligo-Gly-modified SPR chip, transpeptidation occurs, covalently immobilizing β₂AR. The efficacy of the cleavage and transpeptidation reactions was validated through SDS-PAGE, Western blot, and chromatographic analysis. The SPR chip was characterized by scanning electron microscope (SEM), energy dispersive spectroscopy (EDS) mapping, X-ray photoelectron spectroscopy (XPS), and contact angle analysis, while β₂AR activity was evaluated by SPR. When compared to the EDC/NHS-based random method and the haloalkane dehalogenase (HaloTag)-mediated site-specific strategy, β₂AR immobilized through the SrtA-mediated method exhibited higher activity with ligands, demonstrating precision in binding affinity evaluations. This strategy meets the benchmarks for an optimal site-specific immobilization method and holds promise for applications involving the modification of other biological interfaces or biosensors.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117452"},"PeriodicalIF":10.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water-stable perovskite/metallic nanocomposites-based SERS aptasensor for detection of neuron-specific enolase","authors":"Jiayi Miao ,&nbsp;Yifan Liu ,&nbsp;Yi Xiao ,&nbsp;Congzheng Yuan ,&nbsp;Qian Xu ,&nbsp;Panpan Chen ,&nbsp;Yang Jin ,&nbsp;Liying Zhang ,&nbsp;Hongliang He ,&nbsp;Shuhu Du","doi":"10.1016/j.bios.2025.117462","DOIUrl":"10.1016/j.bios.2025.117462","url":null,"abstract":"<div><div>Perovskite/metallic heterojunction-based surface enhanced Raman scattering (SERS) substrates have been proven to be capable of providing Raman enhancement. However, the inherent water instability and poor dispersibility of perovskite/metallic nanocomposites-based SERS substrates pose significant challenges to their application in aqueous environments. Herein, polydopamine (PDA)-encapsulated cesium lead bromide (CsPbBr₃) adsorbing gold nanoparticles (AuNPs), termed as CsPbBr₃@PDA@AuNPs, is prepared as SERS substrate, which exhibits excellent water stability and SERS activity. Dopamine as organic ligand not only passivates surface defects during the growth of perovskite nanocrystals, but also forms porous PDA protective layer, effectively preventing degradation of perovskite in aqueous medium. Meanwhile, PDA with abundant functional groups and conjugated π structure will adsorb AuNPs and promote electron flow between CsPbBr₃ and AuNPs, resulting in strong SERS activity. Based on the results, a SERS aptasensor has been fabricated by conjugation between CsPbBr₃@PDA@AuNPs and double-stranded DNA (dsDNA), which is composed of neuron-specific enolase (NSE) aptamer and partial complementary signal-stranded DNA (ssDNA). The working strategy of as-fabricated SERS aptasensor is based on the conformational change (of ssDNA)-triggered Raman response for the detection of NSE. Upon the addition of NSE, the specific binding of NSE aptamers to NSE can convert rigid dsDNA into a flexible ssDNA, and the Cy5 signal molecule modified at the end of ssDNA will close to CsPbBr₃@PDA@AuNPs SERS substrate, generating significant Raman signals with the lower limit of detection (1.02 pg/mL) of NSE. The SERS aptasensor has broad application prospect in the field of life/medicine science fields (e.g. early diagnosis and screening of disease).</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"280 ","pages":"Article 117462"},"PeriodicalIF":10.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Confining metal nanoparticles and nanoclusters in covalent organic frameworks for biosensing and biomedicine","authors":"Jianxin Ma ,&nbsp;Zhongjie Cai ,&nbsp;Faisal Ahmad ,&nbsp;Yelan Xiao ,&nbsp;Tong Shu ,&nbsp;Xueji Zhang","doi":"10.1016/j.bios.2025.117461","DOIUrl":"10.1016/j.bios.2025.117461","url":null,"abstract":"<div><div>Metal nanoscale particles, primarily including metal nanoparticles (MNPs) and nanoclusters (MNCs), have garnered substantial interests owing to their unique electronic configurations and distinct physicochemical properties. However, practical applications are frequently constrained by their limited stability and aggregation tendency. Covalent organic frameworks (COFs), featuring highly ordered periodic architectures, have emerged as ideal porous matrices for hosting metal nanoparticles. The resulting metal-embedded COFs synthesized through adsorption methods (M/COFs) or <em>in-situ</em> reduction (M@COFs) not only mitigate nanoparticle aggregation and enhance stability but also demonstrate synergistic effects that generate enhanced or novel functionalities, significantly broadening their application potential. This review firstly examines adsorption-based synthesis strategies for M/COFs through physical and chemical approaches. Subsequently, we analyze <em>in-situ</em> reduction methods for M@COFs, categorizing them by reduction pathways: deposition, impregnation-pyrolysis, and “one-step” synthesis. Special attention is given to an emerging pore wall engineering strategy within <em>in-situ</em> reduction approach. The biosensing and biomedical applications of metal-embedded COFs are systematically examined, highlighting their comparative advantages over conventional nanomaterials in sensing and antimicrobial applications. While metal-embedded COFs remain in their developmental infancy and face considerable challenges, the controlled synthesis of multifunctional variants promises transformative potential across biomedical domains.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117461"},"PeriodicalIF":10.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143839231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in situ-boosted electrochemiluminescence biosensor for serotonin detection sensitized with Co3O4 nanoplates and self-feedback DNA recycling","authors":"Yongguang Gao ,&nbsp;Qiumei Feng ,&nbsp;Xiangmin Miao ,&nbsp;Yufei Fu","doi":"10.1016/j.bios.2025.117464","DOIUrl":"10.1016/j.bios.2025.117464","url":null,"abstract":"<div><div>Here, we described an in situ-boosted electrochemiluminescence (ECL) biosensor for serotonin detection sensitized with the catalysis of Co₃O₄ nanoplates and self-feedback DNA recycling (SFDR). Specifically, aptamer:S duplex was attached onto the surface of magnetic beads via amide bond. When serotonin presented, it competitively combined with aptamer from aptamer:S duplex. Then, the released S strands reacted with hairpin DNA (H) containing the silenced Mg²⁺-assisted DNAzyme site in the loop part that immobilized on CdS quantum dots (CdS QDs) modified electrode. The activating Mg²⁺-assisted DNAzyme cut off H and re-liberated S strands. Therefore, the Mg²⁺-DNAzyme-aided recycle happened to produce numerous residual single-stranded DNA segments and then reacted with Co₃O₄-modified P strands on the electrode surface. Such results directly introduced Co₃O₄ nanoplates close to CdS QDs, resulting in the reduction of H₂O₂ to •OH and the increase of ECL emission of CdS QDs in CdS QDs-H₂O₂ system. Based on such signal amplification strategy, a low detection limit of 1.5 pM was obtained for serotonin detection. This approach enabled the proposed ECL biosensor promising for the future application in the trace detection of serotonin.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117464"},"PeriodicalIF":10.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical manipulation of the insulin secretion from pancreatic beta cells directly cultured on a PEDOT:PSS electrode","authors":"Hisakage Funabashi ,&nbsp;Hayate Inoue ,&nbsp;Reiji Shigematsu ,&nbsp;Ichiro Imae ,&nbsp;Yoshiteru Amemiya ,&nbsp;Takenori Ishida ,&nbsp;Takeshi Ikeda ,&nbsp;Ryuichi Hirota ,&nbsp;Akio Kuroda","doi":"10.1016/j.bios.2025.117453","DOIUrl":"10.1016/j.bios.2025.117453","url":null,"abstract":"<div><div>The development of cell-based devices using mammalian cells is becoming increasingly feasible. To remotely control such sophisticated devices, an interface between digital computer/internet networks and cellular/organ networks is essential. This study explores the electrochemical manipulation of insulin secretion—a regulatory hormone for the control of blood sugar levels—using pancreatic β cells as a model. iGL cells, expressing insulin fused with <em>Gaussia</em> Luciferase (INS-GLase), were directly cultured on a custom-made cell culture device coated with a transparent poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) electrode. Luminescence imaging was employed to evaluate insulin secretion in response to applied potentials. Results showed that insulin secretion could be induced by regulating membrane potential through an applied potential. The addition of nicardipine, an L-type voltage-dependent Ca²⁺ channel inhibitor, suppressed insulin secretion, suggesting the involvement of Ca²⁺ channels in this electrochemical system. Additionally, changes in membrane potential were directly visualized with the membrane potential-sensitive dye FluoVolt™, which confirmed both the forced depolarization and the forced restoration of the membrane potential to its non-excited state upon potential application to the electrode. The reported electrochemical technique, in which cells are directly cultured on an electrode, offers significant promise for designing advanced bio-hybrid systems that integrate cellular functions with digital networks.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117453"},"PeriodicalIF":10.7,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly efficient nickel-based metal atom cluster/metal oxide microsensors for the rapid and accurate screening of Helicobacter pylori infection","authors":"Huina Sun ,&nbsp;Wei Jin ,&nbsp;Handong Yao ,&nbsp;Mingshi Deng ,&nbsp;Xuefeng Wang ,&nbsp;Xinghua Gao ,&nbsp;Wanyin Xu ,&nbsp;Qianqian Li ,&nbsp;Yongheng Zhu ,&nbsp;Ning Zhao ,&nbsp;Yuan Zhang","doi":"10.1016/j.bios.2025.117456","DOIUrl":"10.1016/j.bios.2025.117456","url":null,"abstract":"<div><div><em>Helicobacter pylori (H. pylori)</em> is a prevalent bacterium that infects the stomach, can cause numerous gastric diseases, and potentially result in stomach cancer. Current <em>H. pylori</em> detection methods have various limitations; thus, to streamline <em>H. pylori</em> detection, we developed an electrochemical microsensor featuring Ni-based atom cluster (AC)/oxide nanocomposite catalysts for direct biomarker identification. By incorporating Ni ACs and transforming Ni oxides into an ultrathin, porous structure, the resulting material exhibited excellent electrocatalytic activity. In particular, it enabled the detection of urease, a biomarker specific to <em>H. pylori</em>, at concentrations as low as 10 ng/mL. The fabricated Ni AC/NiO@laser-etched graphene (LEG) electrochemical microsensor demonstrated excellent sensitivity and specificity in detecting urease within the concentration range of 10–100 ng/mL. Moreover, its accuracy in analyzing clinical samples matched that of commercial enzyme-linked immunosorbent assay kits, highlighting its potential as a platform for both the personal health monitoring and clinical diagnosis of <em>H. pylori</em> infection. This microsensor exhibited excellent sensitivity and precision and rapid recognition with intuitive operation and ease of use. It holds considerable promise in enhancing and improving medical diagnostics by providing timely and accurate information, enabling earlier interventions, and improving patient outcomes.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"280 ","pages":"Article 117456"},"PeriodicalIF":10.7,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laccase-mimicking enzymes with synergistic amplification effects on catalytic activity for ergothioneine multi-pattern logic analysis","authors":"Meini Li ,&nbsp;Yunfei Xie ,&nbsp;Xingguang Su","doi":"10.1016/j.bios.2025.117457","DOIUrl":"10.1016/j.bios.2025.117457","url":null,"abstract":"<div><div>In this work, a multimodal analysis strategy for ergothioneine (EGT) with a logical structure was constructed utilizing a novel laccase mimic enzyme (CuPH@KMO). CuPH@KMO demonstrated improved catalytic efficiency and an expanded operational pH range, attributed to synergistic interactions. Firstly, CuPH@KMO with laccase activity could not only catalyze the oxidation of the typical substrates 2,4-dichlorophenol (2,4-DP) and 4-aminoantipyrine (4-AP), but also be innovatively employed to oxidize dopamine (DA) and resorcinol (RS) to blue fluorescent azamonardine (Ex = 478 nm) with its absorbance peak located at 420 nm. Then, EGT with reducing and complexing activities could inhibit the CuPH@KMO-catalyzed oxidation of DA and RS, which resulted in the attenuation of both colorimetric and fluorescence signals. Consequently, a multimodal sensing strategy for EGT detection was developed, utilizing CuPH@KMO as the catalytic component, DA and RS as responsive substrates. This method was successfully applied to EGT analysis in cosmetics and dietary supplement. More importantly, a bimolecular logic gate with “AND” and “INHIBIT” had been successfully constructed according to the logical relationship between DA, RS and EGT, which realized the significant process of converting complex data into binary. The implementation of logic sensors based on laccase-mimicking enzymes presents novel opportunities for integrating bioanalysis with logic analysis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117457"},"PeriodicalIF":10.7,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allosteric ribozyme-driven crRNA switch for the amplification-free detection of biomolecules","authors":"Mei Su ,&nbsp;Hong-Shuai Zhang ,&nbsp;Hao Liu ,&nbsp;Kai Yang ,&nbsp;Zhan-Ming Ying","doi":"10.1016/j.bios.2025.117450","DOIUrl":"10.1016/j.bios.2025.117450","url":null,"abstract":"<div><div>Currently, CRISPR-mediated biosensors are concentrating on the design of the crRNA or the activator strand to regulate the trans-cleavage activity of Cas12a. Herein, we report an allosteric ribozyme-driven crRNA switch-regulated CRISPR/Cas12a sensor for amplification-free detection of biomolecules. An allosteric ribozyme is meticulously engineered to connect the target recognition sequence with the 5′ binding arm of the hammerhead ribozyme, resulting in the formation of a hairpin structure through complementary hybridization. The presence of target induces the conformational change in the allosteric module and disrupts the hairpin structure, restoring multiple-turnover cleavage RNA activity of ribozyme. Then, the activated ribozyme specifically cuts the cleavage site of the substrate-locked crRNA and releases the native crRNA to initiate CRISPR/Cas12a functions for signal reporting. The reported biosensor exhibited high sensitivity and excellent specificity for miR-155 and adenosine triphosphate (ATP) detection, giving the detection limits of 256 fM and 160 nM, respectively. For clinical validation, our proposed strategy can quantify miR-155 expression levels in cells and serum of cancer patients. Furthermore, we also demonstrate that the allosteric ribozyme-driven crRNA switch can be easily compatible with lateral flow assays, realizing visualization and the portable monitoring of target. Hence, the biosensor not only has outstanding potential in point-of-care testing, but also enables the detection of various biomolecules by flexibly substituting target recognition sequences for molecular diagnosis in the clinic.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"280 ","pages":"Article 117450"},"PeriodicalIF":10.7,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive magnetic nanomechanical biosensors for simultaneous detection of multiple cardiovascular disease biomarkers in a single blood drop","authors":"Qiubo Chen ,&nbsp;Lin Zhang ,&nbsp;Zihan Qiao ,&nbsp;Yipeng Yang ,&nbsp;Wenjie Wu ,&nbsp;Chen Wang ,&nbsp;Jian Chen ,&nbsp;Shangquan Wu ,&nbsp;Qingchuan Zhang","doi":"10.1016/j.bios.2025.117448","DOIUrl":"10.1016/j.bios.2025.117448","url":null,"abstract":"<div><div>Cardiovascular disease (CVD) is the number one cause of death, and the early prevention of CVD is considered the most useful and cost-effective intervention strategy, highlighting the critical need for frequent and long-term monitoring cardiac abnormalities. However, traditional blood test methods often require considerable volumes of blood (&gt;10 mL), which could burden physical health, especially for individuals in poor health. Here, we report a novel magnetic nanomechanical sensor (MNS) capable of simultaneously detecting multiple CVD biomarkers (brain natriuretic peptide (BNP), cardiac troponin I (cTnI) and creatine kinase MB (CK-MB)) in a single drop of blood (&lt;1 μL). Relying on the force-sensitive microcantilevers and robust magnetic force, MNS can directly detect blood samples with a detection sensitivity for BNP as low as 0.1 pg/mL. Moreover, we improved the MNS sensitivity by reducing nonspecific adsorption and focusing the force on specific locations on the sensor surface. The effectiveness of the MNS was demonstrated through the detection of samples from clinical CVD patients and healthy individuals. Given its ultrasensitive, trace-sample requirement, and ability to monitor multiple biomarkers, the MNS holds significant potential for frequent and long-term monitoring—not only for CVD but also for the prevention and management of other chronic diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"280 ","pages":"Article 117448"},"PeriodicalIF":10.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}