Biosensors and Bioelectronics最新文献_第10页

Transcription cascade reaction-driven isothermal amplification for ultrasensitive detection of high-risk human papillomavirus 转录级联反应驱动的等温扩增用于高危人乳头瘤病毒的超灵敏检测。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-10 DOI: 10.1016/j.bios.2025.117984

Gun Haeng Lee , Subin Kim , Seok Hyeon Kim , Eun Sung Lee , Ki Soo Park

{"title":"Transcription cascade reaction-driven isothermal amplification for ultrasensitive detection of high-risk human papillomavirus","authors":"Gun Haeng Lee , Subin Kim , Seok Hyeon Kim , Eun Sung Lee , Ki Soo Park","doi":"10.1016/j.bios.2025.117984","DOIUrl":"10.1016/j.bios.2025.117984","url":null,"abstract":"<div><div>Rapid and ultrasensitive nucleic acid detection is crucial for reducing spatiotemporal disparities in clinical outcomes. High-risk human papillomavirus (HPV), which is predominantly linked to cervical cancer, requires early and accurate detection for timely medical intervention. Here, we developed R-transcription cascade reaction (TCR), an isothermal detection platform that integrates recombinase polymerase amplification (RPA) with TCR at 37 °C for the rapid and ultrasensitive detection of HPV 16 and HPV 18, both of which are major oncogenic HPV types. R-TCR amplifies RPA products through TCR, generating light-up RNA aptamers, enabling highly specific and amplified fluorescence detection within 40 min for singleplex and 45 min for multiplex assays. The system reaches an exceptional detection limit of 1 aM (21 copies) in both single-target and multiplex formats, without cross-reactivity. It also enables reliable detection of high-risk HPV DNA integrated into the genomic DNA of cervical cancer cells. In clinical samples, R-TCR achieved 100 % sensitivity and specificity in both singleplex and multiplex assays. These results validate the strong potential of R-TCR as a rapid, ultrasensitive, and economical molecular diagnostic tool for high-risk HPV screening, making it especially suitable for use in low- and middle-income countries.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117984"},"PeriodicalIF":10.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microwave biosensor for amylase detection in drainage fluid to monitor anastomotic leakage 微波生物传感器检测引流液中淀粉酶监测吻合口漏

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-10 DOI: 10.1016/j.bios.2025.117990

Maziar ShafieiDarabi , Qianying Mao , Weijia Cui , Matthew Courtney , Robert Miranda , Kulsum Ali , Lauren LeSergent , Ricky Tjandra , Zahra Abbasi , Luke P. Lee , Carolyn L. Ren

{"title":"Microwave biosensor for amylase detection in drainage fluid to monitor anastomotic leakage","authors":"Maziar ShafieiDarabi , Qianying Mao , Weijia Cui , Matthew Courtney , Robert Miranda , Kulsum Ali , Lauren LeSergent , Ricky Tjandra , Zahra Abbasi , Luke P. Lee , Carolyn L. Ren","doi":"10.1016/j.bios.2025.117990","DOIUrl":"10.1016/j.bios.2025.117990","url":null,"abstract":"<div><div>Anastomotic leakage (AL) is a serious complication following colorectal cancer surgery, resulting in increased morbidity, extended hospital stays, and higher mortality rates. Current methods for AL monitoring are often delayed, non-specific, and unsuitable for long-term point-of-care (POC) applications, highlighting the need for cost-effective, portable biosensors. <strong>Amylase, a promising biomarker present in</strong> peritoneal <strong>fluid, offers hope for early and long-term detection of AL.</strong> In this study, we present a miniaturized microwave biosensor capable of quasi-continuous (e.g. hourly) monitoring of amylase concentrations from postoperative day 1 and for a long time. The sensor detects clinically relevant amylase concentrations (>1000 U/L) within an hour, enabling early and prolonged monitoring of AL. High specificity was achieved using an amylopectin coating that reacts selectively with amylase, even in complex samples such as simulated peritoneal fluid (SPF). We validated the performance of our miniaturized, portable microwave sensor against the EnzChek™ Ultra Amylase Assay Kit, confirming its accuracy and reliability. This low-cost, portable biosensor provides a proof-of-concept for a practical, highly specific POC solution for real-time AL monitoring, supporting both early diagnosis and long-term postoperative care.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117990"},"PeriodicalIF":10.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic bead-assisted one-pot RCA-activated CRISPR/Cas12a electrochemiluminescence biosensor for the detection of citrus Huanglongbing pathogen 磁珠辅助一锅rca激活CRISPR/Cas12a电化学发光生物传感器检测柑橘黄龙冰病原菌

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-10 DOI: 10.1016/j.bios.2025.117986

Yingsi He , Yutian Zhang , Hongzhang Xiang , Kejing Ren , Yashi Yin , Yanru Gao , Yingjie Yang , Weidong Zhang , Lingzhi Liu , Heyou Han , Wenjing Wang

{"title":"Magnetic bead-assisted one-pot RCA-activated CRISPR/Cas12a electrochemiluminescence biosensor for the detection of citrus Huanglongbing pathogen","authors":"Yingsi He , Yutian Zhang , Hongzhang Xiang , Kejing Ren , Yashi Yin , Yanru Gao , Yingjie Yang , Weidong Zhang , Lingzhi Liu , Heyou Han , Wenjing Wang","doi":"10.1016/j.bios.2025.117986","DOIUrl":"10.1016/j.bios.2025.117986","url":null,"abstract":"<div><div>Huanglongbing (HLB) poses a catastrophic threat to the global citrus industry, necessitating early detection of pathogen for disease control and minimize economic losses. Herein, we reported a one-pot electrochemiluminescence (ECL) biosensor for integrating rolling circle amplification (RCA)-activated CRISPR/Cas12a dual cleavage activity, and engineered magnetic beads-based quenched ECL emitter. Target-initiated RCA generated amplicons that activated Cas12a, simultaneously leveraging <em>cis</em>-cleavage for template recycling and <em>trans</em>-cleavage to degrade single stranded DNA attached on Ru(bpy)<sub>3</sub><sup>2+</sup>-loaded magnetic beads. This dual-amplification strategy restored ECL signals, enabling ultrasensitive detection of <em>Candidatus Liberibacter asiaticus</em> (<em>C</em>Las) ribonucleotide-diphosphate reductase subunit beta gene fragments with high specificity. A linear range 10 fM-1 nM with the detection limit of 2 fM was obtained. The integrated platform eliminated multi-step incubations, and exhibited satisfactory performance in citrus leaf samples, offering a powerful tool for HLB diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117986"},"PeriodicalIF":10.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term dynamic tracking of acute kidney injury by Au nanoclusters-based reversible NIR-II fluorescent probe 基于金纳米簇的可逆NIR-II荧光探针对急性肾损伤的长期动态跟踪

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-09 DOI: 10.1016/j.bios.2025.117982

Qing Hu , Fanyi Qin , Huijiao Qu, Huayu Xiong, Shengfu Wang, Xiuhua Zhang, Yan Xiao

{"title":"Long-term dynamic tracking of acute kidney injury by Au nanoclusters-based reversible NIR-II fluorescent probe","authors":"Qing Hu , Fanyi Qin , Huijiao Qu, Huayu Xiong, Shengfu Wang, Xiuhua Zhang, Yan Xiao","doi":"10.1016/j.bios.2025.117982","DOIUrl":"10.1016/j.bios.2025.117982","url":null,"abstract":"<div><div>Acute kidney injury (AKI) is a common kidney disease characterized by a sudden decline in renal function. It usually has no clear clinical symptoms in its early stages, but can easily progress to renal failure or even result in death. Real-time monitoring of AKI progression significantly enhances the prospects for early diagnosis and prompt intervention. However, there is no available to monitor AKI as developing a renal-clearable and reversibly responsive probe capable of long-term in vivo track biomarkers of AKI, such as reactive oxygen species (ROS), remains an extremely challenging task. Herein, we have constructed a redox-reversible second near-infrared (NIR-II) fluorescent probe based on Au nanoclusters (Au NCs) for long-term dynamic monitoring of AKI. The probe features NIR-II excitation and emission capabilities, along with effective kidney targeting and prolonged retention, facilitating renal enrichment and enabling long-term high-resolution renal imaging. Notably, it demonstrates rapid and reversible responsiveness to ROS with high sensitivity and selectivity, permitting real-time monitoring of redox fluctuations in vivo. Given its exceptional performance, the probe achieves unprecedented real-time dynamic tracking of AKI progression, providing crucial redox status information during the disease process. Additionally, the probe can serve as an effective new tool for screening of potential therapeutic drugs for AKI. This work may offer some substantial promotion for the diagnosis and treatment of kidney diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117982"},"PeriodicalIF":10.5,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurochemical profiling in urine: Multiplexed detection of dopamine and serotonin using ML-integrated laser-induced graphene biosensors 尿液中的神经化学分析：使用ml集成激光诱导石墨烯生物传感器多路检测多巴胺和血清素

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-09 DOI: 10.1016/j.bios.2025.117981

Hossein Chenani , Vinay Kammarchedu , Heshmat Asgharian , Aida Ebrahimi

{"title":"Neurochemical profiling in urine: Multiplexed detection of dopamine and serotonin using ML-integrated laser-induced graphene biosensors","authors":"Hossein Chenani , Vinay Kammarchedu , Heshmat Asgharian , Aida Ebrahimi","doi":"10.1016/j.bios.2025.117981","DOIUrl":"10.1016/j.bios.2025.117981","url":null,"abstract":"<div><div>Simultaneous monitoring of dopamine (DA) and serotonin (SER) in urine offers a non-invasive route for diagnosing neurological, psychiatric, and metabolic disorders. However, their multiplexed detection at the point-of-care remains challenging due to matrix complexity, low analyte concentrations, and overlapping oxidation potentials which complicates electrochemical testing. In this work, we developed laser-induced graphene (LIG)-based electrochemical sensors for multiplexed detection of clinically-relevant concentrations of DA and SER in undiluted human urine. We first optimized the number of laser passes and electrode size, showing that two-pass LIG improves the sensor performance compared to one-pass LIG. Additionally, Nafion-coated LIG electrodes exhibited high selectivity for DA and SER – over 88% relative to various interfering molecules – while further reducing LODs for both analytes by approximately 10 × compared to uncoated electrodes. While electrode engineering and single-mode voltammetry enabled detection of sub-micromolar DA and SER, we combined multimodal voltammetry with machine learning and achieved detection down to 5 nM for both analytes, corresponding to >60-fold and >120-fold improvements over single-mode voltammetry. The sensors were validated against high-performance liquid chromatography, showing a good agreement with less than 10% relative error. In addition, the sensors achieved recovery rates of 91%–108% which fall within the United States Food and Drug Administration regulatory requirements, highlighting the potential of the developed device for advancing urine analysis at the point-of-need, neuroscience research, and clinical care.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117981"},"PeriodicalIF":10.5,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A quick thermal response digital acoustofluidic system for rapid on-chip PCR 芯片上快速PCR的快速热响应数字声流控系统

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-09 DOI: 10.1016/j.bios.2025.117976

Lei Huang , Mingyong Zhou , Rongda Kang , Luming Li , Dan Li , Bingyan Jiang

{"title":"A quick thermal response digital acoustofluidic system for rapid on-chip PCR","authors":"Lei Huang , Mingyong Zhou , Rongda Kang , Luming Li , Dan Li , Bingyan Jiang","doi":"10.1016/j.bios.2025.117976","DOIUrl":"10.1016/j.bios.2025.117976","url":null,"abstract":"<div><div>Polymerase chain reaction (PCR) has been widely used in gene identification, pathogen detection, tumor research since invented in the last century. Microfluidic chips, which have low sample consumption, contactless operation, and rapid response, have been proven to be an ideal carrier for PCR technology. However, due to the precise temperature control required in the PCR process, additional temperature modules need to be integrated, which will present challenges to the application of microfluidic PCR devices. In this study, we proposed a rapid PCR system based on digital acoustofluidics with low detection limit (as low as 0.5 ng/mL) and rapid thermal response rate (up to 9.4 °C/s). Specifically, the movement of droplets is prevented by a polydimethylsiloxane (PDMS) ring, and the coupling effect of acoustic thermal with acoustic stream is used for heating micro droplets from inside out. We enhanced the temperature rise capability of the droplets by increasing their viscosity through the addition of glycerol, and equilibrium temperature for fluids with different viscosities was fitted. Based on the adjustment of input power, we determined the temperature control scheme for PCR thermal cycling. Compared to the standard PCR process, this digital acoustofluidic system shows a consistent trend and has a higher amplification efficiency (97.23 %), which can achieve 40 cycles in 57 min. We expect this device to provide support for point-of-care testing (POCT) and rapidly nucleic acid testing in the future.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117976"},"PeriodicalIF":10.5,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145057383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

YOLOv5-aided paper-based microfluidic intelligent sensing platform for multiplex sweat biomarker analysis 基于yolov5的纸质微流控智能传感平台用于多种汗液生物标志物分析

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-08 DOI: 10.1016/j.bios.2025.117978

Tengyun Li , Chenxi Gong , Jianhua Zhou , Lu Huang

{"title":"YOLOv5-aided paper-based microfluidic intelligent sensing platform for multiplex sweat biomarker analysis","authors":"Tengyun Li , Chenxi Gong , Jianhua Zhou , Lu Huang","doi":"10.1016/j.bios.2025.117978","DOIUrl":"10.1016/j.bios.2025.117978","url":null,"abstract":"<div><div>Sweat, a biofluid rich in various biomarkers, offers significant potential for non-invasive health monitoring and disease screening. Colorimetric detection is well-suited for multi-analyte quantification and point-of-care testing in sweat analysis, while conventional platforms often suffer from detection inaccuracies due to subjective interpretation and environmental interference. Furthermore, many existing systems rely on complex fabrication or computationally demanding artificial intelligence models, limiting their scalability and practical use in resource-limited settings. To address these challenges, we developed a YOLOv5-aided paper-based microfluidic intelligent sensing platform that integrates an easily fabricated paper-based microfluidic chip, smartphone imaging, and a deep learning framework which attains a mean average precision of 99.5%. This platform provides a cost-effective, portable, and reproducible approach for the detection of multiplex biomarkers in sweat, and its functionality has been successfully validated through the colorimetric analysis of iron ions, chloride ions, and glucose in sweat.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117978"},"PeriodicalIF":10.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A high-throughput photo-electrochromic ratiometric sensing chip for swine enteric coronaviruses detection 用于猪肠道冠状病毒检测的高通量光电致变色比例传感芯片。

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-08 DOI: 10.1016/j.bios.2025.117983

Yi Zou , Liqi Liu , Yanli Zuo , Xujing Feng , Fenying Kong , Yu Gu , Jie Wei , Nan Hao , Kun Wang

{"title":"A high-throughput photo-electrochromic ratiometric sensing chip for swine enteric coronaviruses detection","authors":"Yi Zou , Liqi Liu , Yanli Zuo , Xujing Feng , Fenying Kong , Yu Gu , Jie Wei , Nan Hao , Kun Wang","doi":"10.1016/j.bios.2025.117983","DOIUrl":"10.1016/j.bios.2025.117983","url":null,"abstract":"<div><div>Porcine deltacoronavirus (PDCoV), porcine epidemic diarrhea virus (PEDV), and porcine transmissible gastroenteritis virus (TGEV) are the main swine enteric coronaviruses, which pose significant threats to the swine industry due to their highly contagious nature and the severe clinical symptoms they cause in pigs. In this research, a high-throughput photo-electrochromic (EC) ratiometric sensing chip was developed for simultaneous detection of PEDV, PDCoV and TGEV. The sensing chip consists of three detection channels and one reference channel fabricated by laser etching. Three-dimensional nitrogen-doped graphene nanosheet-loaded ZnIn<sub>2</sub>S<sub>4</sub> (ZnIn<sub>2</sub>S<sub>4</sub>/3DNG) as the photoelectrochemical active materials and antibodies for PEDV, PDCoV and TGEV as immuno-recognition probes were modified on the electron-injection (EI) areas. Prussian Blue (PB) as an electrochromic material is modified on the EC areas. The photogenerated electrons by ZnIn<sub>2</sub>S<sub>4</sub>/3DNG can induce the color change of PB under light illumination. The capture of viruses by their antibodies can hinder the electron transfer and affect the speed of electrochromism. Therefore, based on the ratio of the grayscale values of EC areas in three detection channels and one reference channel, the simultaneous detection of PEDV, PDCoV and TGEV can be achieved in the range of 1.0 × 10<sup>2</sup> TCID 50⋅mL<sup>−1</sup> to 1.0 × 10<sup>5</sup> TCID 50⋅mL<sup>−1</sup>, 5.0 × 10<sup>3</sup> TCID 50⋅mL<sup>−1</sup> to 1.0 × 10<sup>7</sup> TCID 50⋅mL<sup>−1</sup>, and 1.0 × 10<sup>3</sup> TCID 50⋅mL<sup>−1</sup> to 1.0 × 10<sup>7.5</sup> TCID 50⋅mL<sup>−1</sup>, respectively. The sensing chip provides an efficient, portable detection platform for swine enteric coronaviruses.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117983"},"PeriodicalIF":10.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible, multifunctional sensor based on core-sheath sensing medium for humidity sensing and heat-resistant pressure 基于芯套传感介质的柔性多功能传感器，用于湿度传感和耐热压力

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-06 DOI: 10.1016/j.bios.2025.117944

Qianyang Wang , Duixin Ma , Huayang Fang , Di Wu , Jianping Sun

{"title":"Flexible, multifunctional sensor based on core-sheath sensing medium for humidity sensing and heat-resistant pressure","authors":"Qianyang Wang , Duixin Ma , Huayang Fang , Di Wu , Jianping Sun","doi":"10.1016/j.bios.2025.117944","DOIUrl":"10.1016/j.bios.2025.117944","url":null,"abstract":"<div><div>The practical implementation of wearable sensing devices for human health monitoring requires significant advancements in lightweight design and multifunctional integration. Fiber-shaped sensors have attracted considerable research attention due to their ability to maintain exceptional sensitivity and measurement accuracy under various mechanical deformations, including bending, stretching, and torsion. Nevertheless, the functional integration remains constrained, particularly as evidenced by sensitivity degradation and device failure under extreme high-temperature conditions, which severely hinders their practical applicability for real-time health monitoring applications in complex environmental scenarios. Herein, we developed a core-sheath aerogel fibrous multifunctional sensor via a one-step coaxial wet-spinning technique. This sensor integrates humidity sensing capabilities for respiratory monitoring and liquid molecule recognition, along with high-temperature-resistant pressure sensing performance. The fiber-based humidity sensor demonstrates rapid response and ultrahigh sensitivity (3144.74 %/% RH) with excellent repeatability. Beyond enabling real-time respiratory detection, the ANFs@MXene/PVA (AMP) humidity sensor responds effectively to non-contact humidity stimuli and discriminates diverse liquid molecules, showcasing its potential for both contact and non-contact environmental sensing in complex scenarios. Additionally, the aramid nanofiber-based sheath enhances the stability of the fiber sensor as a wearable electronic device under extreme conditions, ensuring its functionality in high-temperature environments. This intelligent core-sheath fiber architecture offers a robust solution for real-time health monitoring in harsh environments, demonstrating significant potential for applications in smart textiles.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117944"},"PeriodicalIF":10.5,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bimodal optical fiber biosensor with high anti-interference based on two-photon polymerization 3D nanofabrication 基于双光子聚合三维纳米制造的高抗干扰双峰光纤生物传感器

IF 10.5 1区生物学

Biosensors and Bioelectronics Pub Date : 2025-09-06 DOI: 10.1016/j.bios.2025.117952

Pengqi Gong , Xuegang Li , Xue Zhou , Yanan Zhang , Yong Zhao

{"title":"Bimodal optical fiber biosensor with high anti-interference based on two-photon polymerization 3D nanofabrication","authors":"Pengqi Gong , Xuegang Li , Xue Zhou , Yanan Zhang , Yong Zhao","doi":"10.1016/j.bios.2025.117952","DOIUrl":"10.1016/j.bios.2025.117952","url":null,"abstract":"<div><div>This study develops a micro/nano dual-mode optical fiber sensor based on two-photon polymerization 3D processing technology. This sensor can simultaneously discriminate changes in bulk refractive index (BRI) caused by interferents and changes in surface refractive index (SRI) triggered by target analytes, making it suitable for biological detection in complex environments. The SRI detection of target biomolecules is achieved through surface plasmon resonance (SPR) assisted by surface biofunctionalization, while the BRI detection is accomplished through the optical path refractive index modulation mechanism of Fabry-Perot interference (FPI) in the resonant cavity. 3D microfabrication technology enables angular modulation of the conical waveguide and fabrication of the open resonant cavity, ensuring the simultaneous excitation of SPR and FPI effects within the micron-scale space. A pressure-driven microfluidic chip facilitates liquid-phase analysis with a sample volume of only 5 μL. Through refractive index solution tests and DNA hybridization experiments, the dual-mode BRI/SRI sensing characteristics were systematically characterized, with detection limits (DL) reaching 4 × 10<sup>−4</sup> RIU and 1.2 nM. A DNA detection experiment using fetal bovine serum (FBS) was performed to simulate simultaneous BRI/SRI changes in biological detection scenarios. A matrix signal analysis method was proposed, which, when combined with dual-mode signal responses, enables accurate analysis of complementary DNA concentration with an error controlled within 3.7 %. In addition, the sensor's consistency and detection specificity across different environments were evaluated.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"290 ","pages":"Article 117952"},"PeriodicalIF":10.5,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0