Biosensors and Bioelectronics最新文献

{"title":"Selective detection of Staphylococcus aureus in food matrices using a chemiluminescent peroxidase-like DNA nanomachine","authors":"Pavel Filatov ,&nbsp;Vladislav A. Reushev ,&nbsp;Ekaterina Shchekutieva ,&nbsp;Georgy Otinov ,&nbsp;Lili Feng ,&nbsp;Tigran Vartanyan ,&nbsp;Daria Gorbenko ,&nbsp;Mikhail V. Zyuzin","doi":"10.1016/j.bios.2026.118408","DOIUrl":"10.1016/j.bios.2026.118408","url":null,"abstract":"<div><div>The development of rapid, sensitive, and specific methods for nucleic acid detection is critical for molecular diagnostics and biosafety. Here, we report the design and validation of a peroxidase-like DNA nanomachine (PxDm) that integrates target-templated assembly of a split G-quadruplex (G4) DNAzyme with chemiluminescent (CL) detection via photon counting in a compact, closed-cuvette device for the accurate detection of <em>S. aureus</em>. Following systematic optimization of the CL reaction, the PxDm platform demonstrated robust analytical performance at room temperature, reaching a nanomolar limit of detection (LOD) for single-stranded DNA (ssDNA) approximately 30 min after the start of the analysis. The assay was shown to be able of discriminating target sequences from those bearing single-nucleotide substitutions (SNS) with up to 98 % signal reduction. This CL approach proved to be more sensitive than colorimetric and fluorescent detection modalities for the same PxDm, with a 19.6-fold and 4.3-fold lower LOD, respectively. The platform's versatility was demonstrated through successful adaptation for double-stranded DNA (dsDNA) detection and integration with dual-priming isothermal amplification (DAMP). The integrated DAMP–PxDm assay achieved clinically relevant sensitivity, with a detection limit of 227 genomic equivalents, while maintaining high selectivity, as indicated by reduction factor (RF) values of 62.1 % for non-target DNA amplicons and 63.9 % for simulated target amplicons containing an SNS. The system demonstrated robustness in complex matrices, accurately detecting <em>S. aureus</em> amplicons from bacteria recovered from lettuce and spinach leaves, even in the presence of non-target organisms. The PxDm platform offers a powerful combination of low-nanomolar sensitivity, single-nucleotide specificity, and sufficient operational time, establishing it as a promising tool for on-site pathogen detection and environmental monitoring applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118408"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145996797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiomyocyte mechanical contraction sensitivity-enhanced biosensing for precise drug evaluation","authors":"Tao Liang ,&nbsp;Zhekun Jia ,&nbsp;Chengyun Wang ,&nbsp;Jiaru Fang ,&nbsp;Dongxin Xu ,&nbsp;Hao Wang ,&nbsp;Ling Zou ,&nbsp;Zhen Wang ,&nbsp;Ning Hu","doi":"10.1016/j.bios.2026.118405","DOIUrl":"10.1016/j.bios.2026.118405","url":null,"abstract":"<div><div>Cardiovascular diseases is the leading cause of mortality worldwide, underscoring the critical importance of preclinical drug screening and cardiac safety in cardiovascular therapeutics to mitigate the immense health and economic burdens. In vitro cardiomyocyte models have been utilized to develop drug evaluation biosensing platforms, leveraging their rhythmic mechanical contractions. However, conventional label-based methodologies pose challenges for long-term monitoring due to the drug adverse effect and phototoxicity, and video-based strategies necessitate high-resolution imaging but is constrained by low frame rates, while label-free biosensing platforms often require costly and time-consuming microfabrication techniques. Here, we introduce a mechanical contraction biosensing platform that incorporates a multi-channel size-regulable impedance sensor (SRIS) with a custom-developed detection system. The fabrication process of SRIS is straightforward and efficient, providing substantial benefits in terms of economic and temporal costs. Further, the SRIS platform strategically regulates three distinct electrode sizes, and the optimal driving frequency of each sensor is systematically determined. Through long-term dynamic cellular viability and contraction assessment, the cardiomyocyte-based contraction model is refined and prepared for drug evaluation. Three typical cardiac drugs are applied to modulate cardiomyocyte contraction function: a receptor agonist, an ion channel blocker, and a myosin inhibitor. The findings suggest that the SRIS platform proficiently captures the effects of both electrode size and drug concentration on cardiomyocyte contraction properties, thereby demonstrating its precise pharmacological assessment. This facilely fabricated, cost-effective, and size-regulable cardiomyocyte contraction biosensing platform holds significant potential for applications in cardiology and pharmacology research.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"298 ","pages":"Article 118405"},"PeriodicalIF":10.5,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AuCu-CuO@rGO-based non-enzymatic electrochromic biosensor powered by ISF for wearable glucose monitoring","authors":"Zhanhong Li ,&nbsp;Haotian Li ,&nbsp;Jingxuan Huang ,&nbsp;Zheyuan Fan ,&nbsp;Wen Sun ,&nbsp;Longzhao Chen ,&nbsp;Linshuang Mu ,&nbsp;Qingyun Han ,&nbsp;Dongyun Ma ,&nbsp;Zhigang Zhu","doi":"10.1016/j.bios.2025.118336","DOIUrl":"10.1016/j.bios.2025.118336","url":null,"abstract":"<div><div>Glucose monitoring is critical for managing diabetes and preventing organ damage. Self-powered biosensors integrating biofuel cells (BFCs) with electrochromic materials (ECMs) can overcome the need for external power supplies and bulky analytical instruments, which is a limitation of conventional glucose meters. However, current research on BFC-ECM-based glucose sensors predominantly uses enzyme-based anodes. In this study, we developed the first wearable, disposable, non-enzymatic, self-powered electrochromic glucose sensor based on AuCu-CuO@reduced graphene oxide (rGO). The device comprises AuCu-CuO@rGO (anode) with an amorphous/crystalline heterostructure, an electrochromic Prussian blue (PB) display (cathode), and a microneedle array for minimally invasive extraction of interstitial fluid glucose. This design leverages the inherent potential difference between the oxidation onset potential of glucose and the formal potential of PB to drive electron transfer with direct colorimetric quantification without an external power source. The device exhibited a peak power density of 0.45 μW cm⁻² and an open-circuit voltage of up to 0.49 V at 12 mM glucose. The detection platform provides dual-mode results output: a semi-quantitative analysis by the naked-eye and an accurate quantification based on the red channel ratio (R/R₀) of the acquired PB display images. The quantitative mode demonstrated linear detection over a range of 4–14 mM (R² = 0.99) at 2 min. In vivo tests demonstrated that the sensor's signal trend was consistent with commercial glucose monitoring. This work provides a self-powered, non-enzymatic, and visually readable system, offering a promising approach for glucose monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"297 ","pages":"Article 118336"},"PeriodicalIF":10.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple microRNAs analysis based on DNAzyme cascade DNA fiber barcodes for cell typing","authors":"Xuewei Li ,&nbsp;Ji Zheng ,&nbsp;Xiaoxing Fang ,&nbsp;Wenqi Gao ,&nbsp;Hejiang Chen ,&nbsp;Zhenyi Liu ,&nbsp;Chao Li ,&nbsp;Ruiping Zhang","doi":"10.1016/j.bios.2026.118383","DOIUrl":"10.1016/j.bios.2026.118383","url":null,"abstract":"<div><div>Accurate tumor subtyping through multiplex miRNA profiling is critical for precision medicine but remains challenging due to limitations in current methods, including cross-reactivity, cost, and stability. Herein, we present a DNAzyme-powered DNA fiber barcode platform that combines G-quadruplex (G4)/hemin catalysis, miRNA-responsive displacement, and polydopamine (PDA)-mediated fluorescence modulation for sensitive and specific miRNA detection. The system operates via a target-dependent “switch”: in the absence of target miRNAs, G4/hemin DNAzymes catalyze dopamine (DA) oxidation into PDA that efficiently quench fluorescence through Förster resonance energy transfer (FRET); when target miRNAs are present, they competitively binding to the capture strands on DNA fibers with higher affinity, displacing the G4 structures, inhibiting DNAzyme formation and preserving the fluorescence signal, with fluorescence intensity showing a positive correlation with target concentration. This mechanism enables dual qualitative and quantitative analysis with a broad linear range (50 pM-100 nM) and low detection limits (5.50 pM). Key advantages include stable, tunable G4/hemin transduction, enhanced kinetics and signal-to-noise through synergistic displacement-quenching, and multicolor barcoding for one-pot multiplex miRNA detection. Validated against qPCR with high concordance, this platform overcomes existing technical barriers to enable robust miRNA-based cell typing classification and precision diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"297 ","pages":"Article 118383"},"PeriodicalIF":10.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145987562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating ferroptosis susceptibility by monitoring lipid peroxidation in endoplasmic reticulum with a tailored fluorescence probe","authors":"Hongyu Li ,&nbsp;Quan Lu ,&nbsp;Yue Jian ,&nbsp;Zihan Chen ,&nbsp;Wen Shi ,&nbsp;Xiaohua Li ,&nbsp;Gang Wei ,&nbsp;Zeli Yuan ,&nbsp;Huimin Ma","doi":"10.1016/j.bios.2026.118377","DOIUrl":"10.1016/j.bios.2026.118377","url":null,"abstract":"<div><div>Evaluating cellular susceptibility to ferroptosis, a regulated cell death process driven by the lipid peroxidation (LPO), is critical for understanding relevant pathogenesis and developing targeted therapies. This objective may be achieved by monitoring LPO within endoplasmic reticulum (ER), which is the earliest site of lipid peroxide accumulation during ferroptosis; however, tools for real-time and <em>in situ</em> detection of LPO in ER remain lacking. Herein, we report an ER-targeting fluorescence probe ER-LPO for monitoring LPO in ER and evaluating ferroptosis susceptibility. ER-LPO exhibited a sensitive, selective and rapid fluorescence off-on response at 518 nm to lipid peroxides, and could monitor both lipoxygenase-catalyzed enzymatic and hydroxyl radical-triggered non-enzymatic LPO. Using ER-LPO imaging, dynamic accumulation of lipid peroxides on the ER membrane was monitored during ferroptosis, which was negatively correlated with cell viability but independent of the induction pathways and cell lines. Notably, increasing polyunsaturated fatty acids (PUFA) in membrane phospholipids through PUFA supplementation or enhancing ACSL4-catalyzed PUFA assimilation accelerated LPO process and heightened cellular susceptibility to ferroptosis. Conversely, supplementing monounsaturated fatty acids or inhibiting ACSL4 to block the PUFA uptake could reduce LPO levels and facilitate ferroptosis resistance of cells. Moreover, ER-LPO enabled high-throughput screening of anti-ferroptosis reagents, identifying baicalein (a trihydroxyflavone derivative) as a potent ferroptosis inhibitor, which may be attributed to its intrinsic antioxidant capacity and lipoxygenase inhibitory activity. As an efficient tool for assessing ferroptosis susceptibility by monitoring of LPO in ER, ER-LPO may offer broad applications in mechanism studies, therapeutic evaluations and new drug developments for ferroptosis-related diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"297 ","pages":"Article 118377"},"PeriodicalIF":10.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered CdS@ZIF67 nanozyme-assisted detection of vitamin D deficiency","authors":"Anna Anandita ,&nbsp;Dharitri Rath ,&nbsp;Premveer Singh ,&nbsp;Prakash Kumar Pathak ,&nbsp;Rahul R. Salunkhe","doi":"10.1016/j.bios.2026.118379","DOIUrl":"10.1016/j.bios.2026.118379","url":null,"abstract":"<div><div>Peroxidases are natural enzymes commonly employed in developing sensitive immunoassays; however, their limited stability has sparked growing interest in enzyme-mimics. Zeolitic imidazolate frameworks (ZIFs), a subclass of metal-organic frameworks (MOFs), are promising alternatives due to their ease of synthesis, tunable porosity, multiple active sites, and robust catalytic properties. In the current work, we synthesized and characterized CdS@ZIF67, which is a nanocomposite of CdS quantum dots and ZIF-67, exhibiting peroxidase-like activity. Catalytic performance was then examined using Michaelis-Menten kinetics, with estimated key parameters (<em>k</em>_<em>cat</em><em>, K</em>_<em>m</em>, and catalytic efficiency <em>k</em>_<em>cat</em><em>/K</em>_<em>m</em>) benchmarked against natural Horseradish Peroxidase (HRP) enzyme. It showed an increase in catalytic efficiency of the order 10³ as compared to ZIF 67, and a comparable catalytic efficiency with that of HRP. To demonstrate its biosensing ability, we further conjugated it to anti-vitamin D3 antibodies and implemented this for sensing vitamin D3 molecules in a competitive dipstick format. Here, the nanozymes catalyzed a clear colorimetric response for clinically relevant concentrations of vitamin D3 in the presence of TMB and H₂O₂, with a limit of detection (LOD) of 26.98 ng/ml, and a LOD of 24.93 ng/ml in spiked serum samples. Our assays could reliably distinguish between deficiency and sufficiency of vitamin D3. This study provides valuable insights into nanozyme design and mimetic function, emphasizing the promising role of CdS@ZIF67 nanozymes as substitutes for natural enzymes in biosensing applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"297 ","pages":"Article 118379"},"PeriodicalIF":10.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a multilayer fluorescence-encoded single quantum dot-based FRET nanosensor for simple and sensitive monitoring of circular RNA in clinical tissues","authors":"Qingyi Wang ,&nbsp;Jinping Hu ,&nbsp;Jun Lu ,&nbsp;Chun-yang Zhang","doi":"10.1016/j.bios.2026.118401","DOIUrl":"10.1016/j.bios.2026.118401","url":null,"abstract":"<div><div>Circular RNAs (circRNAs) represent a new category of evolutionarily conserved covalent-closed RNAs with critical roles in tumorigenesis and progression of various cancers. The accurate and sensitive monitoring of circRNAs is essential for early disease diagnosis and therapeutic monitoring. Herein, we construct a multilayer fluorescence encoding-mediated single QD-based Förster resonance energy-transfer (FRET) nanosensor for the simple and sensitive monitoring of circRNA in breast tumor cells and clinical tissues. This nanosensor involves only a multi-functionalized hairpin switcher probe that integrates circRNA recognition, enzymatic amplification, and signal transduction. When target circRNA is absent, the hairpin switcher probe is tightly locked in an inactive state. The introduction of target circRNA to hybridize with the hairpin switcher probe will induce its conformational change through toehold-mediated strand migration, initiating the Klenow fragment polymerase-assisted sequential encoding of multiple Cy5 fluorophores into the stem of probes. Subsequently, these Cy5-labeled reaction products can be assembled onto 605QDs to form the QD-DNA-Cy5 nanostructures, producing remarkable FRET signals that can be directly counted through single-molecule imaging. Remarkably, this assay can be homogeneously and isothermally carried out without requiring any enrichment or separation steps, and the multilayer fluorescence encoding endows the system with great operation simplicity and high analytical performance. This nanosensor achieves a detection limit of 24.16 aM, and can be extended to monitor intracellular circMTO1 expression at single-cell level. Moreover, it can accurately distinguish breast cancer tumor from healthy counterparts, providing a new platform for clinical diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"297 ","pages":"Article 118401"},"PeriodicalIF":10.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SERS-integrated plasmonic microneedles with yolk-shell nanocomposites for closed-loop management of infected wounds via dual antifouling safeguards","authors":"Juanjuan Zhu ,&nbsp;Yaxian Wang ,&nbsp;Yuzhu Li ,&nbsp;Yueyue Zhao ,&nbsp;Cheng Wang ,&nbsp;Qiong Wang ,&nbsp;Cheng Qu ,&nbsp;Mengke Su ,&nbsp;Honglin Liu","doi":"10.1016/j.bios.2025.118348","DOIUrl":"10.1016/j.bios.2025.118348","url":null,"abstract":"<div><div>The escalating global burden of infected wounds demands transformative theranostic platforms integrating real-time diagnostics with synergistic therapeutics. We present an implantable microneedles (MNs) system synergized with surface-enhanced Raman spectroscopy (SERS) for closed-loop diagnosis and therapy. This platform employs yolk-shell Au nanostars@zeolite imidazolate framework-8 (AuNS@ZIF-8) nanocomposites as a multifunctional core, overcoming fundamental barriers in intravivo nanobiosensing and therapy. SERS and fluorescence quantitative analysis results show that the microneedle layered structural filtration and molecular sieving filterprovide dual antifouling safeguards, ensuring the stability of the biosensor in the complex biological environment. Crucially, the yolk-shell architecture amplifies localized electromagnetic fields driving a 217 % increase in SERS intensity relative to core-shell analogues and enabling precise pH monitoring through 4-mercaptobenzoic acid (4-MBA) protonation shifts. Concurrently, the system facilitates combinatorial therapy; the yolk-shell structure enhances photothermal conversion efficiency, enabling extended thermal retention alongside Zn²⁺-mediated ROS generation. In vitro antibacterial experiments showed that the strategy could effectively inhibit the growth of <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em>. In vivo validation demonstrates accelerated wound healing (50.63 ± 18 % area reduction on day 6), representing, to our knowledge, the first closed-loop diagnostic-therapeutic system based on plasmonic MNs (PMNs) for intravivo applications. By unifying ultrasensitive diagnostics with potent combination therapy in a single implantable device, this work transcends the limitations of conventional nanomaterial approaches through synergistic structural innovation and multifunctional integration, offering a groundbreaking strategy for advanced wound management.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"297 ","pages":"Article 118348"},"PeriodicalIF":10.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145909561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field-deployable modular absorbance analysis platform with standardized electronics for point-of-care diagnostics","authors":"Kyung Won Lee ,&nbsp;Nam Ho Bae ,&nbsp;Kyoung G. Lee ,&nbsp;Donggee Rho ,&nbsp;Sung Eun Seo ,&nbsp;Oh Seok Kwon ,&nbsp;Dongwook Choi ,&nbsp;Jihoon Kim ,&nbsp;Hyun C. Yoon ,&nbsp;Yoo Min Park","doi":"10.1016/j.bios.2025.118343","DOIUrl":"10.1016/j.bios.2025.118343","url":null,"abstract":"<div><div>The growing demand for personalized healthcare and real-time patient monitoring has accelerated the advancement of point-of-care testing (POCT) technologies. Among available analytical methods, the enzyme-linked immunosorbent assay (ELISA) is well-known for its high sensitivity, selectivity, and operational simplicity. However, conventional ELISA systems remain poorly suited for portable use because they require complex optical components and stable power supplies. To overcome these limitations, we developed a modular absorbance analysis platform (MAAP) that simplifies ELISA system by reconfiguration its core functions into standardized, task-specific modules. The platform integrates a white light source, intensity regulator, optical sensor, display, and battery module, enabling compact and field-deployable ELISA operation. As proof of concept, a polydimethylsiloxane (PDMS)-based bioreactive chamber was fabricated to perform a competitive immunoassay for C-peptide, an insulin secretion biomarker. Following disassembly, transport, and reassembly of the modular system, colorimetric signal detection was performed on-site. C-peptide was quantitatively analyzed within a defined concentration range with a measurable detection limit, demonstrating both analytical performance and practical applicability. These results highlight the potential of the proposed modular ELISA platform as a versatile and scalable solution for POCT diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"297 ","pages":"Article 118343"},"PeriodicalIF":10.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145839720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosensors based on liquid biopsy for clinical cancer diagnosis","authors":"Kai Ma ,&nbsp;Xiaoming Sun ,&nbsp;Chongyu Liang ,&nbsp;Meiting Yi ,&nbsp;Lanbo Shen ,&nbsp;Jiandong Zhang ,&nbsp;Zenghao Wang","doi":"10.1016/j.bios.2025.118337","DOIUrl":"10.1016/j.bios.2025.118337","url":null,"abstract":"<div><div>Cancer remains a leading cause of global mortality, with incidence and death rates continuing to rise annually, posing an escalating threat to human health. Early detection is paramount for effective intervention, significantly improving patient survival rates by enabling timely treatment when therapeutic options are most viable. However, conventional diagnostic methodologies, often reliant on sophisticated imaging techniques or centralized laboratory analyses of tissue biopsies, face significant limitations. Consequently, the development of accessible and accurate diagnostic tools is of profound significance for improving cancer management outcomes. Liquid biopsy, a minimally invasive approach analyzing tumor-derived biomarkers in readily accessible bodily fluids (e.g., blood, serum, plasma, urine, saliva), represents a particularly promising application for cancer detection. This comprehensive review critically examines the current landscape of sensors specifically designed for the point-of-care detection of tumor biomarkers in bodily fluids, with a deliberate focus on technologies demonstrating clear clinical relevance. We prioritize the discussion of devices that have undergone rigorous validation using authentic clinical samples (patient-derived fluids) and, where available, systems that have progressed to implementation within clinical practice.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"297 ","pages":"Article 118337"},"PeriodicalIF":10.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}