Lab on a Chip最新文献

A novel microfluidic self-perfusion chip (MSPC) for pumpless 3D cell, microtissue and organoid culture.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-10 DOI: 10.1039/d5lc00030k

Guohua Wu,Di Wu,Wenqi Hu,Qinrui Lu,Yusen Zhou,Jie Liu,Qijun Du,Zhi Luo,Haijie Hu,Hongwei Jiang,Bangchuan Hu,Shuqi Wang

{"title":"A novel microfluidic self-perfusion chip (MSPC) for pumpless 3D cell, microtissue and organoid culture.","authors":"Guohua Wu,Di Wu,Wenqi Hu,Qinrui Lu,Yusen Zhou,Jie Liu,Qijun Du,Zhi Luo,Haijie Hu,Hongwei Jiang,Bangchuan Hu,Shuqi Wang","doi":"10.1039/d5lc00030k","DOIUrl":"https://doi.org/10.1039/d5lc00030k","url":null,"abstract":"Microfluidic systems have revolutionized biological research by enabling precise control over cellular environments at microscale volumes. However, traditional pump-driven systems face challenges such as complexity, cost, cell-damaging shear stress, and limited portability. This study introduces a novel adjustable microfluidic self-perfusion chip (MSPC) that uses evaporation as a driving force, eliminating the need for external pumps. Our design offers improved metabolic waste management and simplified control over fluid dynamics. The chip features adjustable evaporation pore sizes, demonstrating a robust linear relationship (R2 = 0.95) between the pore size and fluid evaporation rate. This ensures consistent fluid flow and effective waste removal, shown by lower ammonia and lactate levels compared to conventional cultures. Its unidirectional flow system and integrated one-way valve maintain cell viability, even under complete evaporation conditions. This innovative platform facilitates the cultivation of complex tissue-like structures, providing a valuable tool for tissue and organ model development, as well as drug screening and toxicity testing. By addressing key limitations of traditional systems, our adjustable MSPC represents a significant advancement in microfluidic cell culture technology, offering improved accessibility and applicability in biological research.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"106 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in microfluidic technology for rapid bacterial detection and inflammation-driven diseases.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-09 DOI: 10.1039/d4lc00795f

Jing Zhang, Yatian Fu, Ching Yin Fong, Haojun Hua, Wei Li, Bee Luan Khoo

{"title":"Advancements in microfluidic technology for rapid bacterial detection and inflammation-driven diseases.","authors":"Jing Zhang, Yatian Fu, Ching Yin Fong, Haojun Hua, Wei Li, Bee Luan Khoo","doi":"10.1039/d4lc00795f","DOIUrl":"https://doi.org/10.1039/d4lc00795f","url":null,"abstract":"Bacterial detection is pivotal for the timely diagnosis and effective treatment of infectious diseases. Microfluidic platforms offer advantages over traditional methods, including heightened sensitivity, rapid analysis, and minimal sample volume requirements. Traditional clinical methods for bacterial identification often involve extended processing times and necessitate high pathogen concentrations, resulting in delayed diagnoses and missed treatment opportunities. Microfluidic technology overcomes these limitations by facilitating rapid bacterial identification at lower biomass levels, thus ensuring prompt and precise treatment interventions. Additionally, bacteria-driven inflammation has been associated with the development and progression of various diseases, including cancer. Elucidating the complex interplay between bacteria, inflammation, and disease is essential for devising effective disease models and therapeutic strategies. Microfluidic platforms have been used to construct in vitro disease models that accurately replicate the intricate microenvironment that bacteria-driven inflammation affects. These models offer valuable insights into bacteria-driven inflammation and its impact on disease progression, such as cancer metastasis and therapeutic responses. This review examines recent advancements in bacterial detection using microfluidics and assesses the potential of this technology as a robust tool for exploring bacteria-driven inflammation in the context of cancer.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Mechanical forces and enzymatic digestion act together to induce the remodeling of collagen fibrils in tumor microenvironment.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-09 DOI: 10.1039/d5lc90037a

Jiling Shi, Aihua Jing, Qinan Yin, Xuewei Zheng, Zhigang Hu, Xibin Jiao, Yaomin Fan, Xiangyang Zu, Jinghua Li, Yanping Liu, Jiayu Zhai, Xiucheng Li, Kena Song

引用次数: 0

On-chip colorimetric assay for determining serum lithium concentration from whole blood.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-09 DOI: 10.1039/d5lc00044k

Carl Olsson, Janosch Hauser, Federico Ribet, Fredrik Wikström, André Görgens, Olof Beck, Martin Schalling, Lena Backlund, Niclas Roxhed

{"title":"On-chip colorimetric assay for determining serum lithium concentration from whole blood.","authors":"Carl Olsson, Janosch Hauser, Federico Ribet, Fredrik Wikström, André Görgens, Olof Beck, Martin Schalling, Lena Backlund, Niclas Roxhed","doi":"10.1039/d5lc00044k","DOIUrl":"https://doi.org/10.1039/d5lc00044k","url":null,"abstract":"Lithium is the first-line treatment for bipolar disorder. However, the narrow therapeutic window of serum (s-)lithium is near its toxicity range, necessitating continuous monitoring of patients, a process involving regular hospital visits. On-demand home sampling could allow for more frequent testing, possibly resulting in safer patient outcomes, further dosage optimization, and increased compliance. This article presents a device that measures the s-lithium concentration from whole blood. The device consists of a single-use cartridge able to conduct on-chip serum filtration, volume-metering and an on-chip colorimetric assay. Spiked whole blood shows good linearity (Pearson's r = 0.96, R2 = 0.92), a limit-of-detection of 0.3 mmol L-1, and an average deviation of 0.05 mmol L-1 (±6%) compared to atomic absorption spectroscopy. The on-chip colorimetric assay has shown to be a promising technique for measuring s-lithium concentration from whole blood and could allow patients to assess lithium levels at home and make the treatment available for new patient groups.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light-based 3D printing and post-treatments of moulds for PDMS soft lithography.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-07 DOI: 10.1039/d4lc00836g

Bastien Venzac

引用次数: 0

Correction: Functionality integration in stereolithography 3D printed microfluidics using a "print-pause-print" strategy.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-04 DOI: 10.1039/d5lc90036k

Matthieu Sagot, Timothée Derkenne, Perrine Giunchi, Yohan Davit, Jean-Philippe Nougayrède, Corentin Tregouet, Vincent Raimbault, Laurent Malaquin, Bastien Venzac

引用次数: 0

Rock-on-a-chip: a novel method for designing representative microfluidic platforms based on real rock structures and pore network modelling.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-04 DOI: 10.1039/d5lc00119f

Pablo A Godoy, Alirza Orujov, Aurora Pérez Gramatges, Saman A Aryana

{"title":"Rock-on-a-chip: a novel method for designing representative microfluidic platforms based on real rock structures and pore network modelling.","authors":"Pablo A Godoy, Alirza Orujov, Aurora Pérez Gramatges, Saman A Aryana","doi":"10.1039/d5lc00119f","DOIUrl":"https://doi.org/10.1039/d5lc00119f","url":null,"abstract":"Microfluidics is a key tool for studying pore-scale phenomena in porous media, with applications in oil recovery and carbon storage. However, accurately replicating rock pore structures in quasi-2D microfluidic platforms remains a challenge. Existing design strategies, including regular and irregular networks, fractal geometries, thin-section imaging, and multi-step methods using CT scans and SEM images, often fail to capture real pore space morphologies. To address these issues, we developed a multi-step workflow that preserves pore morphology and size distributions in quasi-2D microchips (rock-on-a-chip) by generating 2D pore throats from 3D network data of CT-scanned rock samples. The method showed strong agreement between 2D and 3D pore and throat size distributions in both designed patterns and fabricated microchips. A critical factor in achieving accurate pore geometry was precise mask alignment, which enabled the fabrication of microchips with narrower throats for relatively tight reservoir patterns. Permeability regulation was achieved by adjusting inlet areas while maintaining pore and throat size distributions similar to the original 3D subvolume. Flow simulations using the Hagen-Poiseuille equation within the OpenPNM framework showed differences between simulated and experimental permeability, especially in low-permeability designs, which were more sensitive to the etching process. Despite these challenges, the proposed approach minimizes common discrepancies between rock pore space morphologies and quasi-2D microchips, significantly improving the reliability of microfluidic studies for applications requiring accurate pore-scale structures.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ monitoring of barrier function on-chip via automated, non-invasive luminescence sensing.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-04 DOI: 10.1039/d4lc01090f

Bryan G Schellberg, Abigail N Koppes, Ryan A Koppes

引用次数: 0

High throughput drug screening platform utilizing capillary and artery cell layered models based on tumor-vascular cell interactions.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-03 DOI: 10.1039/d4lc00950a

Jihyeon Song, Yeji Lee, Min-Seok Kim, Giheon Ha, WonJun Jang, Ulziituya Batjargal, Younggyun Kim, Han-Jun Kim, Junmin Lee

{"title":"High throughput drug screening platform utilizing capillary and artery cell layered models based on tumor-vascular cell interactions.","authors":"Jihyeon Song, Yeji Lee, Min-Seok Kim, Giheon Ha, WonJun Jang, Ulziituya Batjargal, Younggyun Kim, Han-Jun Kim, Junmin Lee","doi":"10.1039/d4lc00950a","DOIUrl":"https://doi.org/10.1039/d4lc00950a","url":null,"abstract":"Interactions between tumors and adjacent blood vessels are critical in the tumor microenvironment (TME) for influencing angiogenesis and hematogenous metastasis. Understanding these interactions within the native TME is vital for targeting various tumors, including brain tumors, due to the complexities of the blood-brain barrier. Developing an accurate tumor model that includes cell-cell and cell-matrix interactions, as well as blood flow-induced shear stress, is essential for high-throughput screening (HTS) of anti-cancer drugs. Here, we developed a glioblastoma (GBM) model surrounded by vascular cells. The arterial model was constructed by encapsulating GBM spheroids with layers of human smooth muscle cells (SMCs) and human umbilical vein endothelial cells (HUVECs), while the capillary cell layered model used only HUVECs. Comparative analysis with tumors from different organs revealed the significant role for platelet endothelial cell adhesion molecule (PECAM) in GBM-blood vascular cell interactions. Cytokine secretion analysis demonstrated PECAM's impact on tumor-specific angiogenic potential. Testing with anti-cancer drugs revealed increased expression of PECAM-associated proteins, drug resistance cytokines, and genes associated with tumor progression and metastasis. Additionally, we developed a HTS platform by encapsulating these tumor models in hydrogels and subjecting them to media circulation, effectively mimicking the dynamic TME, suitable for cancer treatment research and drug development.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel manual rotating fluid control mechanism in a microfluidic device with a finger-actuated pump for dual-mode sweat sampling.

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-04-03 DOI: 10.1039/d5lc00171d

Mohamed Ishag Hassan Gama, Saminu Abdullahi, Marwa Omer Mohammed Omer, Zhu Yang, Xuzhong Wang, Yousuf Babiker M Osman, Yuhang Liu, Jingzhen Li, Yingtian Li, Xing Gao, Zedong Nie

{"title":"A novel manual rotating fluid control mechanism in a microfluidic device with a finger-actuated pump for dual-mode sweat sampling.","authors":"Mohamed Ishag Hassan Gama, Saminu Abdullahi, Marwa Omer Mohammed Omer, Zhu Yang, Xuzhong Wang, Yousuf Babiker M Osman, Yuhang Liu, Jingzhen Li, Yingtian Li, Xing Gao, Zedong Nie","doi":"10.1039/d5lc00171d","DOIUrl":"https://doi.org/10.1039/d5lc00171d","url":null,"abstract":"Wearable sweat analysis using microfluidics offers a non-invasive approach for real-time health monitoring, with applications in chronic disease management, athletic performance optimization, and early-stage condition detection. However, most existing wearable sweat microfluidic devices are limited to single-mode operation either real-time or on-demand sampling and often lack precise control over sample volume, which compromises analytical accuracy and utility. To address these limitations, we present a novel wearable microfluidic device featuring a manual rotating fluid control mechanism and a finger-actuated pump for dual-mode sweat sampling. The rotational control mechanism directs sweat either into detection chambers for volume-independent sensor reactions or through the finger-actuated pump for precise volume control. The pump incorporates a dedicated collection chamber, enabling sweat accumulation and controlled delivery in a single actuation, ensuring reproducible sample volumes and facilitating on-demand analysis when required. Additionally, the device integrates two reaction chambers for simultaneous dual biomarker detection. Performance validation during a 40 minute exercise session, using a colorimetric glucose assay, demonstrated reliable sweat sampling and on-demand biochemical analysis. These results highlight the device's potential as a practical tool for personalized health monitoring and field applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0