Lab on a Chip最新文献

Enhancing microscale printing accuracy in LCD-based 3D printing using an immobilized release film. 使用固定化释放膜提高基于lcd的3D打印的微尺度打印精度。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-04 DOI: 10.1039/d5lc00452g

Chang Tian, Chaojie Shao, Tiantian Li, Wenya Tang, Peiqi Wu, Qian Xu, Wei Li, Fen Zhang

{"title":"Enhancing microscale printing accuracy in LCD-based 3D printing using an immobilized release film.","authors":"Chang Tian, Chaojie Shao, Tiantian Li, Wenya Tang, Peiqi Wu, Qian Xu, Wei Li, Fen Zhang","doi":"10.1039/d5lc00452g","DOIUrl":"https://doi.org/10.1039/d5lc00452g","url":null,"abstract":"With the development of 3D printing technology, liquid crystal display (LCD)-based 3D printing offers a cost-effective solution for microfluidic device fabrication, yet its microscale precision remains limited. The accuracy of printing molds can be improved by reducing the adhesion force between the cured resin and release film. However, the adhesion force between the LCD screen and release film and the deformation of the release film in the separation process are still ignored. Herein, we propose using an immobilized release film to enhance the printing accuracy in microscale printing for microfabrication. By applying transparent double-sided adhesive tape between the LCD screen and release film, the movement and deformation of the release film can be reduced, the vertical accuracy in the process of microscale 3D printing can be improved, and the error rate in height can be reduced from 20% to 5%. By studying the printing effect under different layer heights, it was found that when the layer height was set as 20-30 μm, the printed micromold matched the design features in both size and side structure. Moreover, microstructures less than 30 μm in width can be obtained. Besides, the reproducibility of the immobilized release film across different resins was confirmed. Furthermore, microfluidic chips used in concentration gradient generation can be obtained with a minimum cross section of 204 μm. Finally, we used the printed mold to fabricate a PDMS chip in the study of silicosis and the preventive effect of NAC in silicosis. Moreover, the mechanism of the preventive effect of NAC was studied. We believe that our fabrication technique with an immobilized release film will facilitate the development of microfluidic technology, and expand the scope and application of microfluidics in research and applications in diverse fields, such as analytical biochemistry, pharmaceuticals, and medicine.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput Single Cell Motility Analysis using Nanowell-in-Microwells 微孔中纳米孔高通量单细胞运动分析

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-04 DOI: 10.1039/d5lc00478k

Pan Deng, Wenze Lyu, Deasung Jang, Kerryn Matthews, Simon P. Duffy, Hongshen Ma

{"title":"High-throughput Single Cell Motility Analysis using Nanowell-in-Microwells","authors":"Pan Deng, Wenze Lyu, Deasung Jang, Kerryn Matthews, Simon P. Duffy, Hongshen Ma","doi":"10.1039/d5lc00478k","DOIUrl":"https://doi.org/10.1039/d5lc00478k","url":null,"abstract":"Cell motility is important to many biological processes including cancer, immune response, and tissue repair. Conventional assays measure bulk cell motility, potentially overlooking important heterogeneity and missing important high motility subpopulations. Here, we introduce a high-throughput single-cell motility assay using nanowell-in-microwell plates to precisely track single cell position and analyze their migratory trajectories. By physically confining individual cells in nanowells, we eliminate cell-cell interactions and simplify cell segmentation and tracking. Using this platform, we characterized the motility of single cells across different culture conditions to identify distinct motility phenotypes. Single-cell trajectory analysis revealed pronounced directional persistence, with cells predominantly maintaining their direction of travel and trajectory along nanowell boundaries. Additionally, our approach facilitates the generation of labeled image datasets suitable for AI models to classify cell motility phenotypes from single-cell images. Together, our platform provides a robust, scalable method to analyze cell motility phenotypes and migration behavior at single-cell resolution.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"7 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144566757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying mechanical opacity as a novel indicator for single-cell phenotyping via integrated dynamic mechanical compression and impedance flow cytometry. 通过综合动态机械压缩和阻抗流式细胞术定量机械不透明度作为单细胞表型的新指标。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-04 DOI: 10.1039/d5lc00489f

Shan-Shan Li, Chun-Dong Xue, Si-Yu Hu, Yong-Jiang Li, Xu-Qu Hu, Zhuo Yang, Kai-Rong Qin

{"title":"Quantifying mechanical opacity as a novel indicator for single-cell phenotyping via integrated dynamic mechanical compression and impedance flow cytometry.","authors":"Shan-Shan Li, Chun-Dong Xue, Si-Yu Hu, Yong-Jiang Li, Xu-Qu Hu, Zhuo Yang, Kai-Rong Qin","doi":"10.1039/d5lc00489f","DOIUrl":"https://doi.org/10.1039/d5lc00489f","url":null,"abstract":"A comprehensive understanding of cellular mechanical heterogeneity is essential for identifying phenotypic variations. Impedance flow cytometry offers a high-throughput, label-free approach to assess single-cell electrical properties, yet current methods focus primarily on undeformed cells and overlook mechanical perturbations that may alter cytoskeletal structure and membrane behavior. Here, we present an integrated system that combines controlled mechanical compression with impedance measurement to quantify mechanical opacity-an electrical metric reflecting membrane permeability under dynamic deformation. This parameter correlates with cytoskeletal integrity and reveals how mechanical stimuli influence electrical responses. Theoretical modeling shows that membrane permittivity and conductivity critically shape frequency-dependent impedance, supporting the use of dual-frequency (500 kHz and 5 MHz) measurements to probe both intra- and extracellular properties. We define a four-parameter feature set (Rsqu, Rsti1, Rsti2, Rrelax) to capture impedance changes during deformation and relaxation, offering a compact and interpretable mechanical signature. Using this system, we demonstrate distinct mechanical opacity profiles among three human cancer cell lines (HeLa, SW1990, BxPC-3), reflecting their inherent biomechanical differences. Fluorescence assays confirm that lower mechanical opacity corresponds to increased membrane permeability, linking electrical measurements to underlying structural changes. Our work establishes mechanical opacity as a dynamic, label-free marker for single-cell mechanics, bridging mechanical stimulation and electrical detection. This approach expands the capability of impedance flow cytometry for applications in cell classification, drug screening, and disease diagnostics.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lossless Altered Histone Modification Analysis System (LAHMAS) 无损组蛋白修饰分析系统（LAHMAS）

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-03 DOI: 10.1039/d5lc00060b

Zachary J. Kauffman, Kevin Koesser, Kyle T. Helzer, Marina N. Sharifi, Erika Heninger, Chao Li, Duane Juang, David Jarrard, Shuang G. Zhao, Michael C. Haffner, David Beebe, Joshua M Lang, Jamie M Sperger

{"title":"Lossless Altered Histone Modification Analysis System (LAHMAS)","authors":"Zachary J. Kauffman, Kevin Koesser, Kyle T. Helzer, Marina N. Sharifi, Erika Heninger, Chao Li, Duane Juang, David Jarrard, Shuang G. Zhao, Michael C. Haffner, David Beebe, Joshua M Lang, Jamie M Sperger","doi":"10.1039/d5lc00060b","DOIUrl":"https://doi.org/10.1039/d5lc00060b","url":null,"abstract":"Miniaturized biological assays using microfluidics have the potential to enhance assay sensitivity, reduce reagent consumption, and increase throughput. However, challenges to miniaturization include increased platform complexity and increased surface to volume ratio leading to risk of evaporation and analyte loss through surface binding. Exclusive Liquid Repellency (ELR) enables open microfluidic systems that minimize these challenges through an oil phase that protects small aqueous volumes from temperature fluctuation and evaporation while eliminating surface fouling that lead to sample loss. Here we report a novel microfluidic platform leveraging ELR and Exclusion-based Sample Preparation (ESP) for the miniaturization of CUT&Tag a complex multistep biological assay. The resultant Lossless Altered Histone Modification Analysis System (LAHMAS) employs a PDMS-silane treated glass surface immersed in silicone oil to facilitate lossless liquid handling and prevent sample evaporation. The device design, compatible with standard laboratory equipment, allows for effective processing of cell inputs as low as 100 cells with higher fidelity than macroscale CUT&Tag facilitating accurate chromatin profiling of low input and rare cell samples.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"51 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144547215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic-enzymatic synergy driven photoelectrochemical aptasensor on a microfluidic chip for sub-pM kanamycin detection. 微流控芯片上用于亚pm卡那霉素检测的磁酶协同驱动的光电化学感应传感器。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-02 DOI: 10.1039/d5lc00450k

Yuchen Shen,YunYi Shi,Juan Wang

{"title":"Magnetic-enzymatic synergy driven photoelectrochemical aptasensor on a microfluidic chip for sub-pM kanamycin detection.","authors":"Yuchen Shen,YunYi Shi,Juan Wang","doi":"10.1039/d5lc00450k","DOIUrl":"https://doi.org/10.1039/d5lc00450k","url":null,"abstract":"The escalating global concern over antibiotic contamination in food chains and aquatic ecosystems demands innovative solutions for rapid, on-site monitoring of residual drugs. This study presents an autonomous microfluidic photoelectrochemical (PEC) biosensing platform that synergizes magnetic purification, enzymatic amplification, and nanohybrid-enhanced signal transduction for field-deployable, ultrasensitive kanamycin (KAN) detection. The system integrates three functional layers: aptamer-functionalized magnetic beads (MBs) for selective KAN isolation, alkaline phosphatase (ALP)-catalyzed hydrolysis of L-ascorbic acid 2-phosphate (AAP) to generate electron-donating ascorbic acid (AA), and a TiO2/Nb2C/carbon nitride (CN) photoanode with a type-II heterojunction architecture for an amplified photocurrent response. This cascaded mechanism achieves a 0.00142 nM detection limit (S/N = 3). Crucially, the polydimethylsiloxane (PDMS)-based microfluidic chip automates critical workflows-including target-probe mixing, dsDNA displacement, MB separation, and ALP-Apt transfer through serpentine channels and pressure-driven flow control, eliminating manual intervention. A wireless PEC module coupled with smartphone-based signal processing enables real-time parameter optimization and data transmission via Bluetooth, removing reliance on external instrumentation. The modular design permits rapid adaptation to diverse targets through interchangeable aptamers, validated via spike-recovery tests in real samples. By unifying enzymatic catalysis, magnetic microfluidics, and nanomaterial-engineered photoelectrochemistry, this work establishes a paradigm for decentralized biosensing that bridges laboratory-grade sensitivity with point-of-need practicality, addressing critical gaps in antibiotic monitoring for food safety and environmental surveillance.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"36 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144533494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring the mechanical responses of tumor metastasis based on a microfluidic chip integrated with an electrochemical detection system. 基于集成电化学检测系统的微流控芯片监测肿瘤转移的力学响应。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-02 DOI: 10.1039/d5lc00563a

Shuqi Chen,Hang Qi,Yuanheng Kuang,Quanning Li,Xuejiao Chen,Yanyan Wang

{"title":"Monitoring the mechanical responses of tumor metastasis based on a microfluidic chip integrated with an electrochemical detection system.","authors":"Shuqi Chen,Hang Qi,Yuanheng Kuang,Quanning Li,Xuejiao Chen,Yanyan Wang","doi":"10.1039/d5lc00563a","DOIUrl":"https://doi.org/10.1039/d5lc00563a","url":null,"abstract":"Monitoring the mechanical responses of tumor cells during migration is crucial for understanding the mechanisms of tumor metastasis. Current studies on cellular mechanical responses primarily utilize microscopic observation techniques, while real-time monitoring cellular responses remains limited. In this work, we present a microfluidic tumor migration chip that incorporates electrochemical impedance spectroscopy to study the mechanical responses of tumor cells. Based on this platform, the impacts of spatial confinement and fluid shear stress on the morphology and migratory capacity of breast cancer tumor cells were evaluated in detail, and it was demonstrated that the morphology, migratory velocity and migratory mode of tumor cells are concurrently modulated by these two mechanical factors. Specifically, moderate spatial confinement and fluid shear stress have been observed to promote the migration of tumor cells and affect the change of their migration mode. Furthermore, electrochemical impedance spectroscopy was employed to evaluate the impedance change of tumor cells under different mechanical stimulation. Based on this detection system, not only the number of migrating cells within the microchannels can be quantified, but the transition of MDA-MB-231 cells to an amoeboid migration mode under tight spatial confinement, as well as the elongation of the cell morphology and transition to a mesenchymal mode due to fluid shear stress, can also be characterized. This platform demonstrates the feasibility of real-time monitoring of cell changes in response to mechanical stimuli, and offers a valuable tool for elucidating the mechanisms underlying cell invasion.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"69 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144533530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Present and Future of Smart Functional Materials as Actuators in Microfluidic Devices 微流控器件中智能功能材料作动器的现状与未来

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-02 DOI: 10.1039/d5lc00259a

Sepideh Izaddoust, Isabel Poves-Ruiz, Enrique Azuaje-Hualde, Daniel Patko, Larisa Florea, Colm Delaney, Lourdes Basabe-Desmonts, Fernando Benito-Lopez

{"title":"Present and Future of Smart Functional Materials as Actuators in Microfluidic Devices","authors":"Sepideh Izaddoust, Isabel Poves-Ruiz, Enrique Azuaje-Hualde, Daniel Patko, Larisa Florea, Colm Delaney, Lourdes Basabe-Desmonts, Fernando Benito-Lopez","doi":"10.1039/d5lc00259a","DOIUrl":"https://doi.org/10.1039/d5lc00259a","url":null,"abstract":"The role of actuators in microfluidic systems is fundamental for accurate measurements and analyses, as they enable precise control over fluid flow by converting various forms of energy—including electrical, thermal, piezoelectric, and electromagnetic—into mechanical motion. The integration of actuators within microfluidic devices facilitates system miniaturization, allowing complex fluidic operations at the microscale. Actuators are essential components in micropumps, micromixers, microvalves, and other fluidic control elements, ensuring accurate handling of very small quantities of liquids. However, the selection of the material type for the actuator is highly dependent on the specific application, as well as on the material composition and structural configuration of the microfluidic device in which it will be integrated. Actuators can feature either moving or static components, and the use of hybrid materials allows for the development of innovative actuation mechanisms. Given the vast range of possible actuator-material combinations, selecting an appropriate actuation strategy is critical for optimal device performance. This review presents recent advancements in microfluidic actuation, with a particular emphasis on material innovations. It explores emerging actuator materials integrated within microfluidic channels, their fabrication and integration methods, activation mechanisms, and functional applications. Additionally, the review provides a comprehensive outlook on promising materials for future microfluidic actuator development.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"70 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Neuronal Circuitry in Neurodegenerative Diseases: from traditional models to cutting-edge techniques 探索神经退行性疾病中的神经回路：从传统模型到尖端技术

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-01 DOI: 10.1039/d5lc00125k

Chiara Ausilio, Annachiara Scalzone, PAOLO Antonio NETTI

{"title":"Exploring Neuronal Circuitry in Neurodegenerative Diseases: from traditional models to cutting-edge techniques","authors":"Chiara Ausilio, Annachiara Scalzone, PAOLO Antonio NETTI","doi":"10.1039/d5lc00125k","DOIUrl":"https://doi.org/10.1039/d5lc00125k","url":null,"abstract":"Current treatments of neurodegenerative diseases primarily address symptoms rather than halting pathology progression. This gap is due to the lack of effective methods for monitoring neural circuitry and dynamics over time. In this context, the development of in-vitro models that more accurately replicate the human brain microenvironment has become essential. Traditional two-dimensional (2D) cell cultures, while providing valuable insights, fail to capture the intricate complexity of the human brain. Recent advancements in neuroscience spotlight the emergence of more sophisticated three-dimensional (3D) models, which can more faithfully recapitulate the intricacies of the brain. This review discusses the evolution of in-vitro brain models, emphasizing the transition from traditional 2D cultures to sophisticated 3D systems, including neurospheroids, brain organoids, assembloids and Micro-Tissue Engineered Neuronal Networks (micro-TENNs). We further highlight the emergence of brain-on-chip platforms, combining microfluidics with cell culture technologies to create precisely controlled environments mimicking the physiological conditions of the human brain. Furthermore, we discuss the application of 3D bioprinting technology enabling the generation of neural constructs with precise control over cell placement. Lastly, we delve into the potential of integrating brain organoids with 3D bioprinting technology, aiming to recapitulate the true three-dimensional complexity of the brain, thereby improving the physiological accuracy of brain models for advancing our understanding of neurodegenerative diseases.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"25 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144521514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

REFRACTIVE INDEX SENSING IN A MONOLITHIC MICRO-OPTOFLUIDIC LITHIUM NIOBATE CHIP 单片微光流控铌酸锂芯片的折射率传感

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-01 DOI: 10.1039/d5lc00074b

Daniel Tochi Nwatu, Sergiy Suntsov, Detlef Kip, Kore Hasse

{"title":"REFRACTIVE INDEX SENSING IN A MONOLITHIC MICRO-OPTOFLUIDIC LITHIUM NIOBATE CHIP","authors":"Daniel Tochi Nwatu, Sergiy Suntsov, Detlef Kip, Kore Hasse","doi":"10.1039/d5lc00074b","DOIUrl":"https://doi.org/10.1039/d5lc00074b","url":null,"abstract":"Lithium niobate is an outstanding material for the realization of fully integrated lab on a chip devices due to its variety of physical properties, such as high electro-optic, acousto-optic, pyro-electric, and nonlinear coefficients, which enable multiple functionalities. As proof of principle for a monolithic optofluidic sensor, we report the fabrication of a refractive index sensor consisting of a femtosecond laser written waveguide crossing a microfluidic channel, which was fabricated in a lithium niobate sample. The microchannel was created by selective etching of a femtosecond laser inscribed structure and the surfaces of the etched channel were smoothed by subsequent annealing. Sensitivity and accuracy of the sensor was determined by refractive index measurements of sucrose solutions with different concentrations and the temperature dependency was investigated with an air-filled channel. The Fabry-Pérot interference spectrum recorded in reflection shows a high contrast of 24 dB, which indicates good optical quality of the cavity. Refractive index steps of 10-3 were measured with an accuracy of 8.5 × 10-5 and a sensitivity of 1215 nm/RIU at a wavelength of 1554 nm. A very low repeatability error was determined by multiple measurements under stabilized temperature conditions.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"15 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144521515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-Petri-dish acoustic vortex tweezers 碟式声波涡旋镊子

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-01 DOI: 10.1039/d4lc00799a

Teng Li, Yingshan Du, Bowen Cai, Michael R. Brooks, Chongpeng Qiu, Zhide Wang, Jiali Li, Luyu Bo, Y. Albert Pan, Zhenhua Tian

{"title":"In-Petri-dish acoustic vortex tweezers","authors":"Teng Li, Yingshan Du, Bowen Cai, Michael R. Brooks, Chongpeng Qiu, Zhide Wang, Jiali Li, Luyu Bo, Y. Albert Pan, Zhenhua Tian","doi":"10.1039/d4lc00799a","DOIUrl":"https://doi.org/10.1039/d4lc00799a","url":null,"abstract":"Acoustic tweezers, with the capability to manipulate tiny objects without physical contact, hold substantial potential for biomedical and biological research. However, current acoustic tweezers platforms face challenges in precise, selective, and multi-degree-of-freedom (multi-DoF) manipulation of objects in Petri dishes, making it difficult to integrate them into typical laboratory workflows. This paper presents an acoustic vortex tweezers platform that enables contactless, precise, multi-DoF, and multifunctional manipulation of micro-to-millimeter-scale objects within a Petri dish. The platform features an acoustic holography-based module, which uses a holographic lens to transform acoustic waves and generate a focused acoustic vortex beam. This beam carries sufficient energy to propagate through a Petri dish's bottom wall, creating a ring-shape intensity field for trapping tiny objects. Using lenses encoded with different topological charge numbers, vortex beams with varying diameters can be generated, allowing for trapping various-sized objects. Additionally, in combination with a 3-DoF linear motion module, our integrated platform enables high-resolution translation of acoustically trapped objects along complex paths. We experimentally demonstrated our platform's diverse capabilities, including concentrating micro-objects, trapping flowing micro-objects to create an agglomerate, translating a microparticle and an agglomerate along complex paths, as well as trapping, rotating and translating a zebrafish larva in horizontal and vertical postures. With these capabilities, we expect our in-Petri-dish acoustic vortex tweezers to emerge as a valuable tool for the contactless, high-resolution, programmable handling of tiny biomaterials in biomedical and biological research.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"2 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144521232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0