Lab on a Chip最新文献

{"title":"Thrombolytic Potential of “Hydrodynamic Cavitation on a Chip” Concept: Insights into Clot Degradation","authors":"Abuzer Alp Yetisgin, Beyzanur Ozogul, Unal Akar, RABİA MERCİMEK, Seyedali Seyedmirzaei Sarraf, Tugrul Elverdi, Ehsan Amani, Dmitry Grishenkov, Ali Kosar, Morteza Ghorbani","doi":"10.1039/d5lc00482a","DOIUrl":"https://doi.org/10.1039/d5lc00482a","url":null,"abstract":"Thrombolysis is essential for treating vascular conditions such as pulmonary embolism and deep vein thrombosis, yet current thrombolytic drug-based approaches have notable limitations in efficacy and safety. Hydrodynamic cavitation (HC) offers drug-free clot degradation through mechanical disruption. In this study, the effects of HC exposure on thrombolysis were investigated using Clot-on-a-Chip (CoC) platform. In this regard, the thrombolytic potential of HC exposure was evaluated by analyses involving hemolysis and fibrinolysis. Furthermore, the results were compared with Acoustic Cavitation (AC), a widely studied alternative. According to the obtained results, HC exposure (482 kPa, 120 s) resulted in 12.1% released hemoglobin and a 53.4% reduction in clot mass. In contrast, AC exposure (24 kHz, 50% amplitude, 30 s) led to a 1.3-fold greater mass reduction with 26.8% released hemoglobin, likely due to additional thermal effects. Morphological analyses revealed that HC treatment significantly reduced red blood cell density in a pressure- and time-dependent manner. Notably, HC treatment effectively eroded blood clots by hemolysis with slight fibrinolysis, whereas clot erosion in AC was primarily due to hemolysis. HC achieved thrombolysis comparable to or better than AC, offering a safer, more targeted strategy. The findings will advance mechanistic understanding of cavitation-induced clot degradation and support HC’s clinical potential for thrombosis treatment.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"17 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-chip particle levitation and micromanipulation using bulk acoustic waves","authors":"Emilie Vuille-dit-Bille, Marc-Alexandre Dubois, Junsun Hwang, Dara Bayat, Thomas Overstolz, Amit Dolev, Sarah Heub, Gilles Weder, Michel Despont, Mahmut Selman Sakar","doi":"10.1039/d5lc00747j","DOIUrl":"https://doi.org/10.1039/d5lc00747j","url":null,"abstract":"Acoustofluidic technologies enable precise manipulation of microscale objects using travelling and standing sound waves in physiological fluids, offering exciting capabilities for biomedical and chemical applications. In particular, surface acoustic wave-based devices have shown great promise for on-chip micromanipulation, but their planar transducer configuration limits the usable workspace near the microchannel surface. Here, we present a novel acoustofluidic platform based on a digitally addressable array of piezoelectric micromachined ultrasound transducers (PMUTs) that generate bulk acoustic waves and acoustic traps within three-dimensional (3D) fluidic chambers. Through a combination of finite element modelling and experimental measurements, we quantify the acoustic field distribution and study acoustic trap formation dynamics. We demonstrate deterministic 3D levitation of particles in water at rest and under continuous flow by generating standing acoustic waves across the height of the chamber. Our results show that 30 µm polystyrene particles can be levitated to a pressure node generated 640 µm above the surface with less than 6% positional error. The system applies in-plane acoustic radiation forces as high as 90 pN to keep the particles in the trap under flow rates up to 40 µL/min. We leverage spatiotemporal modulation of the acoustic field for continuous planar transport of microparticle aggregates. PMUT arrays are microfabricated using conventional cleanroom techniques, thus can be readily integrated with compact fluidic systems. Our work lays the foundation for the development of reconfigurable and scalable acoustofluidic micromanipulation systems, with broad potential for lab-on-chip technologies.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"101 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated continuous-flow microfluidic sensor for long-term monitoring of microalgae growth in a tubular photobioreactor","authors":"R. Rahul, Nikhil Prasad, R. S. Mini, S. Kumar Ranjith","doi":"10.1039/d5lc00546a","DOIUrl":"https://doi.org/10.1039/d5lc00546a","url":null,"abstract":"<em>Spirulina</em> (<em>Arthrospira platensis</em>) is a valuable cyanobacterium used for various applications, including health supplements, cosmetics, biofertilizers, carbon capture, and biofuels. Efficient monitoring of microalgae growth in photobioreactors is crucial for optimizing yields in large-scale culturing. Existing monitoring systems take samples from the bioreactor at different intervals and perform the visualization and quantification of algae growth parameters. In this work, a microfluidic platform is mounted on a tubular photobioreactor, and the system continuously monitors the growth behavior of <em>Spirulina</em> over several days, with algal development captured on demand. Furthermore, the microfluidic sensor is fabricated using a novel xurography-based approach on photopolymer sheets. It captures real-time micrographs of algae continuously for 5 days (over 120 hours) under two different conditions: open-loop and closed-loop. In the open-loop configuration, the sensor hydrostatically taps the algal medium from the bioreactor at regular intervals. In contrast, the closed-loop sensor continuously (24/7) circulates the culture medium through the microchip for visualization without the use of any driving mechanism. From the micrographs, algal cell density, cell count, and trichome length are estimated continuously, and all parameters exhibited an increasing trend over time. Importantly, the cell density obtained from the microfluidic sensor closely matches with the conventional benchmark glass slide method, with an error of less than 3.3%. The microfluidic monitoring platform is found to be low-cost, accurate, fast, and efficient compared to existing systems, and moreover, it is easily amenable to automation.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"9 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Building multiple microenvironmental niches using a customizable 3D printed well insert","authors":"Laura A Milton, Surasak Kasetsirikul, Jorge Amaya Catano, Sofia Hilmi, Zeheng Zhou, Thomas G. Molley, Kristopher Kilian, Louis Ong, James Chirnside, Nicholas Byrom, Georgia Balshaw, Sammy Liang, Laura Jane Bray, Dietmar Hutmacher, Christoph Meinert, Yi-Chin Toh","doi":"10.1039/d5lc00753d","DOIUrl":"https://doi.org/10.1039/d5lc00753d","url":null,"abstract":"The increasing demand for advanced in vitro models that replicate physiological crosstalk between cell types within and between organs requires customized cellular microenvironments arranged within a single platform. Hydrogels are biomaterials that mimic the physicochemical properties of tissue niches to optimally support diverse cell types. However, they require integration with cell patterning platforms like bioprinting or microfluidics to create organized multi-niche environments. There is currently a gap between bioprinting, which is scalable but limited by availability of printable hydrogels, and microfluidic patterning, which is compatible with diverse biomaterials but is challenging to multiplex. Here, we developed the Localized Microenvironment Well-Insert (LM-Well), a 3D-printed device designed to pattern multiple hydrogel niches with customizable physicochemical properties in multi-well plates. The LM-Well features patterning structures that enable capillary force-driven patterning of various hydrogel formulations, including natural, photo-crosslinkable and synthetic click hydrogels. Functional materials, exemplified by oxygen-scavenging microcapsules, can be patterned within the LM-Well offering an additional layer of control over local oxygen levels in individual cell niches, which modulated tumor spheroid growth and zonation of hepatic activities. Micro-architectural supports, such as micropillars or scaffolds, can be integrated into the LM-Well to optimally support mechanically-active cells like myocytes. The LM-Well's multi-niche patterning capability enabled the establishment of a liver-tumor co-culture in a single well, recapitulating altered drug efficacy on MCF-7 tumor cells following activation of tamoxifen and deactivation of doxorubicin by HepaRG-derived hepatocytes. As a versatile and accessible platform, the LM-Well facilitates physiologically relevant co-cultures with customizable niches and diverse biomaterials.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"28 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplexed detection of respiratory viral pathogens by isothermal amplification on an autonomously loaded chip at the point-of-care","authors":"Beatrise Berzina, Krishna Gupta, Rayan Suliman, Peter Mirtschink, Alexander Dalpke, Carsten Werner, Elisha Krieg, Lars David Renner","doi":"10.1039/d5lc00509d","DOIUrl":"https://doi.org/10.1039/d5lc00509d","url":null,"abstract":"Recent viral outbreaks have shown the need for reliable diagnostic platforms to rapidly detect various viral and bacterial pathogens at the point-of-care. Over the last decade, isothermal nucleic acid amplification methods have emerged as an appealing alternative to standardized polymerase chain reaction (PCR) tests due to their high sensitivity, selectivity,low cost, and simple assay setup. Virtually, all the nucleic acid testing platforms require a labor-intensive sample preparation step, limiting the scalability and usability of recent alternatives. This article describes multiplexed isothermal detection of respiratory viruses on a valve-free, autonomously loading microfluidic platform—VirChip. We demonstrate that an optimized loop-mediated isothermal amplification (LAMP) enables the simultaneous detection of SARS-CoV-2, Influenza A, Influenza B, and RSV (A/B) with a limit of detection of 100 RNA copies per reaction. The platform is highly selective, as no cross-reactivity amongst the targeted pathogens was observed in patient samples. Furthermore, crude nasal swab samples can be directly applied to the chip, eliminating the requirement for expensive and laborious RNA isolation and sample workup. VirChip facilitates rapid, inexpensive, and multiplexed detection, allowing pathogen screening by primary care providers not only in hospitals but also in resource-limited areas.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"78 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated and Turnkey Custom-Built Multiplexed Portable Platform for On-Site Electrochemical Detection","authors":"Parvathy Nair, Aditya Balasubramanian, Sharan K, Souvik Roy, Ponnalagu R N, Sanket Goel","doi":"10.1039/d5lc00732a","DOIUrl":"https://doi.org/10.1039/d5lc00732a","url":null,"abstract":"A compact and cost-effective device is essential to bring multiplexed biosensing from lab to the point-of-care. Electrochemical sensing has emerged as a powerful choice for developing such lab-on-chip (LOC) platforms, offering good sensitivity, scalability, and compatibility. Potentiostat is a fundamental instrument widely employed in electrochemical sensors for clinical diagnostics, environmental monitoring, immunosensors, toxicity testing, and so on. However, conventional electrochemical workstations are often bulky and expensive, limiting their feasibility for portable, cost-effective biosensing, particularly for multiplexed detection at the point of care. Hence, it is ill-suited for the integration with lab-on-chip systems. This paper presents, a low-cost, portable potentiostat with multiplexed electrochemical biosensing capability and Wi-Fi connectivity designated as µBIOPOT. This operates within a potential window of ±1 V and a current range of ±1 mA, supporting various voltammetric techniques, including Cyclic Voltammetry (CV), Linear Sweep Voltammetry (LSV), Differential Pulse Voltammetry (DPV), Square Wave Voltammetry (SWV), and Normal Pulse Voltammetry (NPV). Its multiplexed capability enables real-time detection of multiple analytes, addressing key challenges in rapid and comprehensive biosensing. Validation of performance is done using the redox probe ferri/ferrocyanide, with results demonstrating close alignment with those obtained with a commercially available conventional potentiostat. Additionally, the device is tested for its ability to detect two key lipid biomarkers—cholesterol and triglycerides, demonstrating its ability for multiplexed detection. User-friendly interfaces provide real-time characteristic plots for research and simplified options for non-experts to determine biomarker concentrations. With Wi-Fi connectivity, low power consumption, and affordability at just $ 36, µBIOPOT represents a scalable, accessible, and efficient solution for the development of multiplexed electrochemical biosensors in both clinical and resource-limited settings.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"214 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pressurized photobonding for 3D-printed inertial and droplet microfluidics.","authors":"Vivek Rajasenan,Edwin Sanchez Ochoa,Aiden Begole,Amrith Karunaratne,Lisa F Horowitz,Albert Folch,Dino Di Carlo","doi":"10.1039/d5lc00433k","DOIUrl":"https://doi.org/10.1039/d5lc00433k","url":null,"abstract":"Microfluidics has transformed scientific and industrial applications by leveraging fluid dynamics at small scales, yet limitations in fabrication techniques continue to impede scalability and design flexibility. This study introduces a novel 3D-printing-based fabrication method, termed \"press-cure\", which enables the creation of microfluidic devices with high resolution, strong bonds, and solvent resistance using commercially available stereolithography printers. By applying uniform pressure and ultraviolet curing to 3D-printed components, the press-cure method achieves sub-100-micrometer channel dimensions, robust bonding, and structural fidelity under pressures exceeding 300 psi. We demonstrate the versatility of this technique through several microfluidic applications, including scalable step emulsifiers for droplet generation, crescent-shaped particle fabrication, and inertial focusing nozzles. The press-cure method overcomes conventional limitations of PDMS and other fabrication materials, offering enhanced geometric complexity, mechanical robustness, and chemical compatibility. This accessible and scalable approach expands the capabilities of additive manufacturing in microfluidics, paving the way for innovative designs in fields such as flow cytometry, microparticle fabrication, and droplet-based assays.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"75 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myo-MOVES: a custom electrical stimulation system for functional studies of 3D bioengineered muscle.","authors":"Martín Ruiz-Gutiérrez,Ainoa Tejedera-Villafranca,Sergi Pujol-Pinto,Javier Ramón-Azcón,Juan M Fernández-Costa","doi":"10.1039/d5lc00614g","DOIUrl":"https://doi.org/10.1039/d5lc00614g","url":null,"abstract":"Electrical pulse stimulation (EPS) is used to replicate motor neuron activation in muscle tissues, enabling in vitro studies of muscle contraction. However, both custom-built and commercial existing EPS systems often suffer from significant limitations, including limited scalability, high cost, and lack of flexibility for experimental adaptation. This work presents the Myo-MOVES platform, a practical solution for stimulating 3D skeletal muscle tissues. The device has been designed as an intuitive EPS system consisting of two main components: a selector and a stimulator that adapts to commercial 24-well culture plates. The Myo-MOVES selector enables targeted stimulation of single or multiple wells, while the stimulator delivers electrical signals via graphite electrodes to the plate containing 3D skeletal muscle samples. The Myo-MOVES platform was technically validated and employed as a proof of concept to investigate sarcolemmal damage induced by muscle contraction in Duchenne muscular dystrophy (DMD) 3D skeletal muscle tissues. Taking advantage of the versatility of the device, we validated Myo-MOVES through the assessment of force generation in DMD engineered muscle tissues and the detection of contraction-induced sarcolemmal damage via Evans blue dye uptake and the release of creatine kinase (CK), the gold standard marker of muscle damage. These findings demonstrate the feasibility of using Myo-MOVES to induce and study functionally relevant disease phenotypes in DMD 3D skeletal muscle tissues. These results highlight the system's potential as a valuable tool for future applications in the field of 3D skeletal muscle tissue engineering, including drug screening and the study of DMD therapies and other muscular diseases.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulus-Induced Mechanical Compaction of Biological Polymer Networks via Smart Hydrogel Microstructures","authors":"Vicente Salas-Quiroz, Katharina Esch, Katja Zieske","doi":"10.1039/d5lc00477b","DOIUrl":"https://doi.org/10.1039/d5lc00477b","url":null,"abstract":"The remodeling of the extracellular matrix by mechanical forces plays a crucial role in organizing cellular microenvironments. To study these mechanical perturbations, various methods have been developed to modify the cellular microenvironment and to apply controlled forces. However, most existing approaches rely either on instruments that cannot be integrated into lab-on-chip systems or on small probes with limited spatiotemporal precision. In this work, a lab-on-chip system enables spatially and temporally controlled mechanical perturbations of biological polymer networks. First, thermoresponsive hydrogel microstructures within flow chambers are fabricated and their material composition and photopolymerization parameters are optimized. Second, the expansion of hydrogel microstructures upon a temporally controlled temperature stimulus, results in compression of Matrigel and collagen networks. Following compression, Matrigel is plastically deformed, whereas the collagen network relaxes elastically. Finally, the compression of collagen networks is spatially modulated by integrating hydrogel structures responsive to light stimuli. By mimicking the pushing forces of cells that remodel biological polymer networks, the presented smart hydrogel microstructures provide a versatile system for future studies on extracellular matrix remodeling and the effects of mechanical forces on cellular microenvironments in both physiological and pathological contexts.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"100 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Programmable Cell Culture Chips for Topographical Manipulation of Living Cells","authors":"Xin-Yi Wu, Jian-Miao Zhang, Meng-Yao Niu, Fan-Chun Bin, Qi Duan, Jie Liu, Xian-Zi Dong, Mei-Ling Zheng","doi":"10.1039/d5lc00803d","DOIUrl":"https://doi.org/10.1039/d5lc00803d","url":null,"abstract":"The micro-morphological characteristics of biomaterial surfaces play a critical role in influencing cell proliferation, adhesion, and differentiation. However, the underlying mechanisms by which surface features modulate cellular behavior remain inadequately understood. Moreover, current surface designs intended for cell regulation tend to be overly simplistic, often failing to meet the dual requirements of high-precision fabrication and structural versatility. Here, we propose a programmable cell culture chip based on femtosecond laser maskless optical projection lithography (Fs-MOPL) technology to modulate the cell behavior. The as-fabricated chip exhibits high structural fidelity and uniformity. Surface treatment with O2 plasma followed by poly-D-lysine (PDL) coating enhances hydrophilicity, cell adhesion and growth. We have investigated the migration, adhesion, and morphological changes of 786-O cells on scaffold with varied line spacing, column diameter and hole size using immunofluorescence staining and confocal fluorescence microscopy. The cells cultured on linear array structures display elongated, oriented actin stress fibers, while column and hole array structures influence focal adhesion distribution and cellular tension. Biocompatibility characterization further confirms the chip's suitability for cell culture applications. Our findings highlight the potential of programmable cell culture chips to mimic complex in vivo microenvironments, offering a multifunctional platform for studying cell behavior and advancing biomedical research.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"23 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}