Lab on a Chip最新文献

Design and Characterization of a Self-Powered Microneedle Microfluidic System for Interstitial Fluid Sampling 一种用于组织间质流体采样的自供电微针微流控系统的设计与表征

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-08-01 DOI: 10.1039/d5lc00590f

Christopher Sharkey, Angélica F. Aroche, Isabella G. Agusta, Hannah Nissan, Tamoghna Saha, Sneha Mukherjee, Jack Twiddy, Michael D. Dickey, Orlin Velev, Michael Daniele

{"title":"Design and Characterization of a Self-Powered Microneedle Microfluidic System for Interstitial Fluid Sampling","authors":"Christopher Sharkey, Angélica F. Aroche, Isabella G. Agusta, Hannah Nissan, Tamoghna Saha, Sneha Mukherjee, Jack Twiddy, Michael D. Dickey, Orlin Velev, Michael Daniele","doi":"10.1039/d5lc00590f","DOIUrl":"https://doi.org/10.1039/d5lc00590f","url":null,"abstract":"Dermal interstitial fluid (ISF) is a promising source of biomarkers for point-of-care (PoC) diagnostics, yet noninvasive and reliable extraction remains a significant challenge. In this study, we present a fully passive microneedle (MN) platform that integrates hydrogel-forming MNs, a hydrogel-based osmotic pump, and paper microfluidics to enable zero-power ISF extraction and analyte transport from skin models. The system’s performance was evaluated using paper microfluidic designs optimized for both bulk fluid uptake and lateral flow-based detection. Osmotic pumping with glycerol and glucose showed comparable extraction efficiencies. Cortisol, a representative stress biomarker, was successfully recovered following 15-minute, 45-minute, and 24-hour sampling durations, demonstrating the platform’s suitability for both short-term and extended ISF monitoring. These results highlight the potential of this integrated MN system as a simple, cost-effective, and minimally invasive solution for passive ISF sampling and subsequent biochemical analysis.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"11 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144756417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Centrifugal microfluidic chip with an air gap for oil-free production of enhanced adipogenic multicellular microspheres. 带气隙的离心微流控芯片，用于无油生产增强型成脂多细胞微球。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-08-01 DOI: 10.1039/d5lc00467e

Xueqing Ren,Xin Wang,Xiaolu Cai,Yi Zou,Peng Chen,Bi-Feng Liu,Yiwei Li

{"title":"Centrifugal microfluidic chip with an air gap for oil-free production of enhanced adipogenic multicellular microspheres.","authors":"Xueqing Ren,Xin Wang,Xiaolu Cai,Yi Zou,Peng Chen,Bi-Feng Liu,Yiwei Li","doi":"10.1039/d5lc00467e","DOIUrl":"https://doi.org/10.1039/d5lc00467e","url":null,"abstract":"Hydrogel microspheres, derived from natural or synthetic materials, serve as crucial platforms for three-dimensional (3D) cell culture and tissue engineering. While traditional production methods like emulsification and microfluidics are widely used, they often involve complex processes and oil phases that can compromise biocompatibility. Here, we present a novel centrifugal microfluidic device with an air gap for producing hydrogel microspheres. Centrifugal force provides a driving force for uniform parallel channels, enabling high-throughput microsphere generation while ensuring size uniformity. The system enables precise size control through centrifugal speed modulation, producing microspheres with diameters ranging from 140.6 ± 17.3 μm to 417.1 ± 34.4 μm with a coefficient of variation below 4.8%. The air gap within the microchannel establishes a step-structure that enables oil-free microsphere generation while ensuring biocompatibility. Moreover, by blending a collagen solution into sodium alginate as the matrix, oil-free microspheres with an interpenetrating polymer network (IPN) can be fabricated, which exhibit excellent biocompatibility to support the culture and adipogenic differentiation of mesenchymal stem cells (MSCs). When cells are cultured with a microsphere-formed scaffold, they exhibit aggregation behavior for enhanced cell-cell communication, which further elevated their adipogenic differentiation potential. Overall, this simplified, high-throughput approach offers a unique platform for applications in cell delivery, drug screening, and tissue engineering.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"721 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144756170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A high-speed sequential liquid compartmentalization method for digital loop-mediated isothermal amplification in a microfluidic device 一种用于微流控装置中数字环路介导等温扩增的高速顺序液体分区方法

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-31 DOI: 10.1039/d5lc00486a

Riku Honda, Taketo Saruwatari, Daigo Natsuhara, Yuka Kiba, Shunya Okamoto, Moeto Nagai, Masashi Kitamura, Takayuki Shibata

{"title":"A high-speed sequential liquid compartmentalization method for digital loop-mediated isothermal amplification in a microfluidic device","authors":"Riku Honda, Taketo Saruwatari, Daigo Natsuhara, Yuka Kiba, Shunya Okamoto, Moeto Nagai, Masashi Kitamura, Takayuki Shibata","doi":"10.1039/d5lc00486a","DOIUrl":"https://doi.org/10.1039/d5lc00486a","url":null,"abstract":"Accurate and rapid quantification of nucleic acid targets is crucial for molecular diagnostics, particularly in resource-limited settings where simple and robust technologies are required. This study presents a high-throughput digital loop-mediated isothermal amplification (dLAMP) platform for the absolute quantification of nucleic acids in a sample, using a microfluidic device comprising ten thousand nanoliter-scale reaction microchambers. The polydimethylsiloxane (PDMS)-based device achieved complete liquid compartmentalization within 60 s in a single operation using an electronic pipette, without requiring surface modification, pre-degassing, pre-priming, or external pumping systems, which are typically necessary in conventional methods. The aqueous sample/reagent mixture was reliably compartmentalized using fluorinated oil, with 97% of the microchambers successfully filled to at least 80% of their designed volume, exhibiting excellent volumetric uniformity (CV = 0.07). Fluorescent LAMP assays targeting Salmonella and cannabis exhibited strong correlations between estimated and true DNA concentrations (R² > 0.98), although quantification was consistently underestimated. Correction factors of 1,000 and 10,000 were required for synthetic Salmonella and cannabis DNA, respectively, whereas only 10 were needed for cannabis seed-derived DNA, indicating these discrepancies were due to the intrinsic performance of the LAMP assays rather than device limitations. The dLAMP device also enabled the successful detection of cannabis seed DNA in the presence of 10 ng µL⁻¹ humic acid, which inhibits amplification in conventional turbidity-based LAMP, demonstrating its robustness for point-of-care testing (POCT) applications. The distinctive compartmentalization strategy of the pipette-operated dLAMP platform enables high scalability without compromising operational simplicity, achieving high throughput, wide dynamic range, and accurate quantification.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"136 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a Diagnostic Platform based on a Spatially Resolved Electrochemiluminescence Immunoassay for Low-Plex Biomarker Detection at Point-of-Care: Mild Traumatic Brain Injury and Cardiac Applications 设计一个基于空间分辨电化学发光免疫分析法的诊断平台，用于在护理点检测低plex生物标志物：轻度创伤性脑损伤和心脏应用

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-30 DOI: 10.1039/d5lc00360a

Milica Jović, Denis Prim, Gabriel Paciotti, Marc Pfeifer

{"title":"Engineering a Diagnostic Platform based on a Spatially Resolved Electrochemiluminescence Immunoassay for Low-Plex Biomarker Detection at Point-of-Care: Mild Traumatic Brain Injury and Cardiac Applications","authors":"Milica Jović, Denis Prim, Gabriel Paciotti, Marc Pfeifer","doi":"10.1039/d5lc00360a","DOIUrl":"https://doi.org/10.1039/d5lc00360a","url":null,"abstract":"Advancements in diagnostics and disease management rely on measuring biomarkers in physiological samples. While multiplex biomarker detection holds great promise for improving disease detection, monitoring, and treatment, developing robust, user-friendly platforms capable of sensitive, decentralized analysis remains a significant challenge. In this article, we describe the development of a next-generation POC diagnostic platform capable of simultaneously quantifying multiple biomarkers from low-volume samples in a highly sensitive way. The platform incorporates a spatially resolved electrochemiluminescence immunoassay (SR-ECLIA) conceived on a single carbon electrode (allowing up to 50 individual biomarker spots/replicates to be realized simultaneously), a disposable microfluidic 3D printed cartridge engineered to handle the assay, and an advanced demonstrator tabletop ECL read-out device with application software for data acquisition and image analysis. The remarkable performance of the platform was demonstrated with the detection of two independent biomarker panels, one for mild traumatic brain injury and one for a cardiac application, with low, double-digit picogram per milliliter limits of detection (1 – 30 pg mL-1). The proposed platform can be mass-produced at a low cost, and it is fundamentally adaptable to measuring other disease-related biomarker combinations, which could open new medical diagnostic avenues for sensitive low-plex biomarker testing at Point-of-Care (xPOCT).","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"52 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liquid Transport Strategies in Wearable and Implantable Microfluidic Systems 可穿戴和可植入微流体系统中的液体输送策略

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-30 DOI: 10.1039/d5lc00593k

Qi Wang, Yizhen Jia, Jinghua Li

{"title":"Liquid Transport Strategies in Wearable and Implantable Microfluidic Systems","authors":"Qi Wang, Yizhen Jia, Jinghua Li","doi":"10.1039/d5lc00593k","DOIUrl":"https://doi.org/10.1039/d5lc00593k","url":null,"abstract":"An essential capability of lab-on-a-chip systems is the precise handling, management, and transport of fluids within microfluidic channels. However, conventional rigid pump–tube–valve systems are often incompatible with emerging wearable and implantable devices, which demand miniaturization, low power consumption, high level of integration, and biocompatibility to ensure reliable and safe operation in biological environments. In recent years, various microscale fluid management and transport strategies have been developed to address these challenges, enabling actively programmable control and significantly advancing the capabilities of bio-integrated electronics. This review summarizes key advances in design architectures, performance control, and integration strategies across four actuation modes: passive, mechanical, pressure-mediated, and electric field–driven mechanisms. Emphasis is placed on their respective advantages and limitations in key application scenarios such as sensing, drug delivery, and biofluid sampling. Finally, we outline potential future directions including device format, comfort level, user safety, and sustained operation, aiming to provide a strategic reference for the development of next-generation fluid management modules in soft bioelectronic systems.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"91 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Lung Tumor-on-a-Chip Model Recapitulates the Effect of Hypoxia on Radiotherapy Response and FDG-PET Imaging 一个肺肿瘤芯片模型概括了缺氧对放疗反应和FDG-PET成像的影响

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-30 DOI: 10.1039/d5lc00373c

Rohollah Nasiri, Myra Kurosu Jalil, Veronica Ibanez Gaspar, Andrea Sofia Flores Perez, Hieu Thi Minh Nguyen, Syamantak Khan, Sindy K Y Tang, Yunzhi Peter Yang, Guillem Pratx

{"title":"A Lung Tumor-on-a-Chip Model Recapitulates the Effect of Hypoxia on Radiotherapy Response and FDG-PET Imaging","authors":"Rohollah Nasiri, Myra Kurosu Jalil, Veronica Ibanez Gaspar, Andrea Sofia Flores Perez, Hieu Thi Minh Nguyen, Syamantak Khan, Sindy K Y Tang, Yunzhi Peter Yang, Guillem Pratx","doi":"10.1039/d5lc00373c","DOIUrl":"https://doi.org/10.1039/d5lc00373c","url":null,"abstract":"Most solid tumors contain regions of hypoxia that pose a significant challenge to the efficacy of radiation therapy. This study introduces a novel 3D lung tumor-on-a-chip (ToC) model designed to replicate the hypoxic tumor microenvironment in vitro while also providing a platform for clinically relevant interventions such as radiotherapy and positron emission tomography (PET) imaging. To simulate the heterogeneous oxygen distribution found in tumors, the ToC model incorporates an oxygen gradient achieved through a straightforward chemical oxygen scavenging system. A unique innovation of this device is the integration of a thin scintillator plate, which enables high-resolution imaging of tumor metabolism under hypoxia and normoxia conditions through radioluminescence microscopy using clinically approved PET tracers such as fluorodeoxyglucose (FDG). The response of this hypoxic model to radiation therapy (10 Gy X-ray) demonstrated a ∼4-fold increase in radioresistance compared to the normoxic ToC model, as assessed by colony formation potential. Additionally, DNA damage observed in the normoxic ToC model was ∼5-fold higher than that in the hypoxic model. platforming addition, the metabolic consumption of glucose was found to mirror the localization of hypoxia, validating the use of this biomarker for planning radiation therapy. The integration of high-resolution radionuclide imaging within ToC models enables on-chip PET imaging and facilitates oncology research and discovery, offering innovative capabilities for the preclinical testing of novel cancer therapies in a clinically relevant environment.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"97 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating Caplacizumab’s Potential to Mitigate Thrombosis Risk in Aortic Valve Stenosis: A Microfluidic and Computational Approach 评估Caplacizumab降低主动脉瓣狭窄血栓形成风险的潜力：微流体和计算方法

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-30 DOI: 10.1039/d5lc00361j

Saeedreza Zeibi Shirejini, Mehrdad Khamooshi, Damien Riska, Martin Nikolov, Marjan Azimi, Shiyen L. Perera, Josie Carberry, Karen Alt, Shaun Gregory, Christoph Hagemeyer

{"title":"Evaluating Caplacizumab’s Potential to Mitigate Thrombosis Risk in Aortic Valve Stenosis: A Microfluidic and Computational Approach","authors":"Saeedreza Zeibi Shirejini, Mehrdad Khamooshi, Damien Riska, Martin Nikolov, Marjan Azimi, Shiyen L. Perera, Josie Carberry, Karen Alt, Shaun Gregory, Christoph Hagemeyer","doi":"10.1039/d5lc00361j","DOIUrl":"https://doi.org/10.1039/d5lc00361j","url":null,"abstract":"Aortic valve stenosis is a progressive cardiovascular disease associated with increased thrombotic risk due to abnormal blood flow patterns. Current management often culminates in valve replacement surgery, demonstrating the need for less invasive therapeutic options. This study investigates the potential of caplacizumab, a von Willebrand factor (vWF) inhibitor, in mitigating thrombosis risk in a microfluidic model of aortic valve stenosis. We employed a novel microfluidic model simulating the hemodynamics of healthy, moderate, and severe stenotic conditions, complemented by computational fluid dynamics simulations (CFD) and conventional platelet function assays. Microfluidic experiments revealed that shear gradients play a critical role in platelet aggregation, with accumulation intensifying as stenosis severity increased, even under constant peak shear rates. Caplacizumab demonstrated high specificity for vWF-mediated platelet activation, significantly inhibiting ristocetin-induced aggregation while not affecting ADP-induced aggregation. At an effective concentration (30 nM), caplacizumab reduced platelet coverage by up to 90% in high shear conditions (4500 s-1) and effectively mitigated shear gradient-dependent platelet aggregation across all stenotic conditions. These findings highlight caplacizumab's therapeutic potential for thrombosis prevention in patients with aortic valve stenosis, offering a foundation for personalized antithrombotic approaches that could potentially reduce thrombotic complications associated with the disease.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"20 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical-driven Miniature Robots: Driving Mechanism, Applications and Future Trends 光驱动微型机器人：驱动机理、应用及未来趋势

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-29 DOI: 10.1039/d5lc00621j

Xiaowen Wang, Sen Jia, Yingnan Gao, Changyou Liu, Yaping Wang, Anqin Liu, Wenguang Yang

引用次数: 0

Accelerated maturation of branched organoids confined in collagen droplets 支状类器官在胶原液滴中的加速成熟

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-29 DOI: 10.1039/d5lc00287g

Iris Elke Ruider, Anna Pastucha, Marion K. Raich, Wentao Xu, Yan Liu, Maximilian Reichert, David A. Weitz, Andreas R. Bausch

{"title":"Accelerated maturation of branched organoids confined in collagen droplets","authors":"Iris Elke Ruider, Anna Pastucha, Marion K. Raich, Wentao Xu, Yan Liu, Maximilian Reichert, David A. Weitz, Andreas R. Bausch","doi":"10.1039/d5lc00287g","DOIUrl":"https://doi.org/10.1039/d5lc00287g","url":null,"abstract":"Droplet-based organoid culture offers several advantages over conventional bulk organoid culture, such as improved yield, reproducibility, and throughput. However, organoids grown in droplets typically display only a spherical geometry and lack the intricate structural complexity found in native tissue. By incorporating singularized pancreatic ductal adenocarcinoma cells into collagen droplets, we achieve the growth of branched structures, indicating a more complex interaction with the surrounding hydrogel. A comparison of organoid growth in droplets of different diameters showed that while geometrical confinement improves organoid homogeneity, it also impairs the formation of more complex organoid morphologies. Thus, only in 750 μm diameter collagen droplets did we achieve the consistent growth of highly branched structures with a morphology closely resembling the structural complexity achieved in traditional bulk organoid culture. Moreover, our analysis of organoid morphology and transcriptomic data suggests an accelerated maturation of organoids cultured in collagen droplets, highlighting a shift in developmental timing compared to traditional systems.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"7 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144719787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inverse 3D ‘lab-on-a-chip’ polymeric microfilms for selective capture of circulating tumor cells from patients’ blood 逆向3D“芯片实验室”聚合微膜用于选择性捕获患者血液中的循环肿瘤细胞

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-07-26 DOI: 10.1039/d4lc01105h

Rituja Gupta, Saloni Andhari, Semonti Nandi, Tanvi Deshpande, Narendra Kale, Chandrashekhar Digambar Bobade, Gourishankar Aland, Sreeja Jayant, Atul Bharde, Aravindan Vasudevan, Pankaj Chaturvedi, Kumar Prabhash, Yuvraj Patil, Jayant Khandare

{"title":"Inverse 3D ‘lab-on-a-chip’ polymeric microfilms for selective capture of circulating tumor cells from patients’ blood","authors":"Rituja Gupta, Saloni Andhari, Semonti Nandi, Tanvi Deshpande, Narendra Kale, Chandrashekhar Digambar Bobade, Gourishankar Aland, Sreeja Jayant, Atul Bharde, Aravindan Vasudevan, Pankaj Chaturvedi, Kumar Prabhash, Yuvraj Patil, Jayant Khandare","doi":"10.1039/d4lc01105h","DOIUrl":"https://doi.org/10.1039/d4lc01105h","url":null,"abstract":"Engineering inverse 3D polymeric microfilms with controlled spatial hierarchy is highly challenging and important at the crossroads of biology and material science due to its potential in enhancing selective cell surface interactions such as in cell adhesion and growth. Protein-modified inverse 3D polymeric microfilms, could thus similarly promote selective cell capture and adhesion. We report the fabrication of inverse 3D polymeric microfilms using composite polymeric-bioligand conjugated films for enhanced capture of circulating tumor cells (CTCs) from cancer patients’ blood. The microfilms were designed using functionalized poly(lactic acid) (PLA) and poly(lactic-co-glycolic acid) (PLGA), mediated with ligand transferrin. Protein immobilization on the films was achieved by conjugating transferrin (Tf), collagen (Co), and bovine serum albumin (BSA) for promoting cellular adhesion and capture. The films were evaluated using scanning electron microscopy (SEM), infrared spectroscopy (ATR-IR) and contact angle (θ) measurements, and demonstrated micro-pores ranging from 18-26 µm. Confocal laser scanning microscopy (CLSM) revealed enhanced cell attachment on the polymeric-blend microfilms evidencing enhanced cell adhesion, capture, and further the ability to proliferate in the 3D space. The inverse 3D polymeric microfilms demonstrated an 80% cell capture efficiency using cultured cancer cells. In a clinical utility, the CTC capturing efficiency was comparable with OncoDiscover® CTC enumeration technology. The inverse 3D polymeric microfilms present a novel ‘lab-on-a-chip’ platform to enable enumeration of CTCs for monitoring minimal residual disease (MRD), progress of metastasis, response to treatment, and finally in early detection of relapse.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"90 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144710857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0