Lab on a Chip最新文献

{"title":"Enhancing commercially available immunoassays through a customized electrokinetic biomolecular preconcentration device†","authors":"Barak Sabbagh, Sinwook Park and Gilad Yossifon","doi":"10.1039/D5LC00179J","DOIUrl":"10.1039/D5LC00179J","url":null,"abstract":"<p >Immunoassays are widely utilized in various settings, from clinics and emergency rooms to remote and resource-limited environments, such as patients' homes. However, they often present a significant trade-off: while offering simplicity, speed, and cost-effectiveness, they generally lack sensitivity. This study introduces an innovative electrokinetic preconcentration device that employs ion concentration-polarization in micro- and nanofluidic systems to continuously capture and preconcentrate target biomolecules. The system then facilitates their transfer with minimal dilution to standard immunoassays, increasing analyte concentration and enhancing transport kinetics and immunoreaction rates. Designed for cost-effectiveness and ease of use, the device does not require sophisticated equipment such as pumps, making it suitable for point-of-care (POC) commercially available immunoassays. By using this device as a preliminary step for various immunoassays, including enzyme-linked immunosorbent assay (ELISA) and lateral flow assay (LFA), we achieved substantial signal enhancement, improving the limit of detection of these assays by an order of magnitude. The obtained enhancement factor correlated with processed sample volume, operational duration, and assay binding kinetics. Our experimental validations and theoretical analysis lay the groundwork for enhancing commercially available immunoassays without modifying the assays themselves, promising significant advancements in biomedical POC diagnostics.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 18","pages":" 4765-4775"},"PeriodicalIF":5.4,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00179j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Programmable 3DP Microfluidic Bio-Reaction System: Automated LAMP-on-a-Chip","authors":"Mehmet Tugrul Birtek, Nazente Atçeken, Savas Tasoglu","doi":"10.1039/d5lc00003c","DOIUrl":"https://doi.org/10.1039/d5lc00003c","url":null,"abstract":"Point-of-care (PoC) devices have revolutionized healthcare by enabling remote diagnostics and therapeutics, with microfluidic systems playing a pivotal role in their advancement. This study focuses on the detailed engineering and characterization of three-dimensional hydrophobic valves to form novel programmable bio-reaction reservoirs. Using 3D-printed soft lithography, we meticulously investigated the effects of channel dimensions and surface properties on the burst pressures of these reservoirs, which ranged from 6.4 to 44.8 mbar. The bio-reaction reservoirs were demonstrated in both series and parallel configurations, offering versatile platforms for the miniaturization and automation of biological processes. Our findings highlight the capability of these reservoirs to program flows in a variety of fluid samples, including water, blood and serum. Additionally, a portable pressure pump was developed to leverage the functionality of these hydrophobic valves, enabling precise control of fluid dynamics in PoC applications. The study culminated in the design of a microfluidic chip integrating two consecutive reservoirs for the PoC execution of Loop-mediated Isothermal Amplification (LAMP) for detection of the Mpox virus. Primers were lyophilized within the bio-reservoirs, and the system achieved visible colorimetric detection of Mpox DNA.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"86 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and characterization of a self-powered microneedle microfluidic system for interstitial fluid sampling","authors":"Christopher T. Sharkey, Angélica F. Aroche, Isabella G. Agusta, Hannah Nissan, Tamoghna Saha, Sneha Mukherjee, Jack S. Twiddy, Michael D. Dickey, Orlin Velev and Michael A. Daniele","doi":"10.1039/D5LC00590F","DOIUrl":"10.1039/D5LC00590F","url":null,"abstract":"<p >Dermal interstitial fluid (ISF) is a promising source of biomarkers for point-of-care (PoC) diagnostics, yet noninvasive and reliable extraction remains a significant challenge. In this study, we present a fully passive microneedle (MN) platform that integrates hydrogel-forming MNs, a hydrogel-based osmotic pump, and paper microfluidics to enable zero-power ISF extraction and analyte transport from skin models. The system's performance was evaluated using paper microfluidic designs optimized for both bulk fluid uptake and lateral flow-based detection. Osmotic pumping with glycerol and glucose showed comparable extraction efficiencies. Cortisol, a representative stress biomarker, was successfully recovered following 15-minute, 45-minute, and 24-hour sampling durations, demonstrating the platform's suitability for both short-term and extended ISF monitoring. These results highlight the potential of this integrated MN system as a simple, cost-effective, and minimally invasive solution for passive ISF sampling and subsequent biochemical analysis.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 18","pages":" 4577-4587"},"PeriodicalIF":5.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00590f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144756417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Centrifugal microfluidic chip with an air gap for oil-free production of enhanced adipogenic multicellular microspheres†","authors":"Xueqing Ren, Xin Wang, Xiaolu Cai, Yi Zou, Peng Chen, Bi-Feng Liu and Yiwei Li","doi":"10.1039/D5LC00467E","DOIUrl":"10.1039/D5LC00467E","url":null,"abstract":"<p >Hydrogel microspheres, derived from natural or synthetic materials, serve as crucial platforms for three-dimensional (3D) cell culture and tissue engineering. While traditional production methods like emulsification and microfluidics are widely used, they often involve complex processes and oil phases that can compromise biocompatibility. Here, we present a novel centrifugal microfluidic device with an air gap for producing hydrogel microspheres. Centrifugal force provides a driving force for uniform parallel channels, enabling high-throughput microsphere generation while ensuring size uniformity. The system enables precise size control through centrifugal speed modulation, producing microspheres with diameters ranging from 140.6 ± 17.3 μm to 417.1 ± 34.4 μm with a coefficient of variation below 4.8%. The air gap within the microchannel establishes a step-structure that enables oil-free microsphere generation while ensuring biocompatibility. Moreover, by blending a collagen solution into sodium alginate as the matrix, oil-free microspheres with an interpenetrating polymer network (IPN) can be fabricated, which exhibit excellent biocompatibility to support the culture and adipogenic differentiation of mesenchymal stem cells (MSCs). When cells are cultured with a microsphere-formed scaffold, they exhibit aggregation behavior for enhanced cell–cell communication, which further elevated their adipogenic differentiation potential. Overall, this simplified, high-throughput approach offers a unique platform for applications in cell delivery, drug screening, and tissue engineering.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 18","pages":" 4756-4764"},"PeriodicalIF":5.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144756170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A high-speed sequential liquid compartmentalization method for digital loop-mediated isothermal amplification in a microfluidic device","authors":"Riku Honda, Taketo Saruwatari, Daigo Natsuhara, Yuka Kiba, Shunya Okamoto, Moeto Nagai, Masashi Kitamura and Takayuki Shibata","doi":"10.1039/D5LC00486A","DOIUrl":"10.1039/D5LC00486A","url":null,"abstract":"<p >Accurate and rapid quantification of nucleic acid targets is crucial for molecular diagnostics, particularly in resource-limited settings where simple and robust technologies are required. This study presents a high-throughput digital loop-mediated isothermal amplification (dLAMP) platform for the absolute quantification of nucleic acids in a sample, using a microfluidic device comprising ten thousand nanoliter-scale reaction microchambers. The polydimethylsiloxane (PDMS)-based device achieved complete liquid compartmentalization within 60 s in a single operation using an electronic pipette, without requiring surface modification, pre-degassing, pre-priming, or external pumping systems, which are typically necessary in conventional methods. The aqueous sample/reagent mixture was reliably compartmentalized using fluorinated oil, with 97% of the microchambers successfully filled to at least 80% of their designed volume, exhibiting excellent volumetric uniformity (CV = 0.07). Fluorescent LAMP assays targeting <em>Salmonella</em> and cannabis exhibited strong correlations between estimated and true DNA concentrations (<em>R</em><small>²</small> &gt; 0.98), although quantification was consistently underestimated. Correction factors of 1000 and 10 000 were required for synthetic <em>Salmonella</em> and cannabis DNA, respectively, whereas only 10 were needed for cannabis seed-derived DNA, indicating these discrepancies were due to the intrinsic performance of the LAMP assays rather than device limitations. The dLAMP device also enabled the successful detection of cannabis seed DNA in the presence of 10 ng μL<small>⁻¹</small> humic acid, which inhibits amplification in conventional turbidity-based LAMP, demonstrating its robustness for point-of-care testing (POCT) applications. The distinctive compartmentalization strategy of the pipette-operated dLAMP platform enables high scalability without compromising operational simplicity, achieving high throughput, wide dynamic range, and accurate quantification.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 18","pages":" 4787-4799"},"PeriodicalIF":5.4,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00486a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering a Diagnostic Platform based on a Spatially Resolved Electrochemiluminescence Immunoassay for Low-Plex Biomarker Detection at Point-of-Care: Mild Traumatic Brain Injury and Cardiac Applications","authors":"Milica Jović, Denis Prim, Gabriel Paciotti, Marc Pfeifer","doi":"10.1039/d5lc00360a","DOIUrl":"https://doi.org/10.1039/d5lc00360a","url":null,"abstract":"Advancements in diagnostics and disease management rely on measuring biomarkers in physiological samples. While multiplex biomarker detection holds great promise for improving disease detection, monitoring, and treatment, developing robust, user-friendly platforms capable of sensitive, decentralized analysis remains a significant challenge. In this article, we describe the development of a next-generation POC diagnostic platform capable of simultaneously quantifying multiple biomarkers from low-volume samples in a highly sensitive way. The platform incorporates a spatially resolved electrochemiluminescence immunoassay (SR-ECLIA) conceived on a single carbon electrode (allowing up to 50 individual biomarker spots/replicates to be realized simultaneously), a disposable microfluidic 3D printed cartridge engineered to handle the assay, and an advanced demonstrator tabletop ECL read-out device with application software for data acquisition and image analysis. The remarkable performance of the platform was demonstrated with the detection of two independent biomarker panels, one for mild traumatic brain injury and one for a cardiac application, with low, double-digit picogram per milliliter limits of detection (1 – 30 pg mL-1). The proposed platform can be mass-produced at a low cost, and it is fundamentally adaptable to measuring other disease-related biomarker combinations, which could open new medical diagnostic avenues for sensitive low-plex biomarker testing at Point-of-Care (xPOCT).","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"52 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liquid transport strategies in wearable and implantable microfluidic systems","authors":"Qi Wang, Yizhen Jia and Jinghua Li","doi":"10.1039/D5LC00593K","DOIUrl":"10.1039/D5LC00593K","url":null,"abstract":"<p >An essential capability of lab-on-a-chip systems is the precise handling, management, and transport of fluids within microfluidic channels. However, conventional rigid pump-tube-valve systems are often incompatible with emerging wearable and implantable devices, which demand miniaturization, low power consumption, high level of integration, and biocompatibility to ensure reliable and safe operation in biological environments. In recent years, various microscale fluid management and transport strategies have been developed to address these challenges, enabling actively programmable control and significantly advancing the capabilities of bio-integrated electronics. This review summarizes key advances in design architectures, performance control, and integration strategies across four actuation modes: passive, mechanical, pressure-mediated, and electric field-driven mechanisms. Emphasis is placed on their respective advantages and limitations in key application scenarios such as sensing, drug delivery, and biofluid sampling. Finally, we outline potential future directions including device format, comfort level, user safety, and sustained operation, aiming to provide a strategic reference for the development of next-generation fluid management modules in soft bioelectronic systems.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 17","pages":" 4252-4272"},"PeriodicalIF":5.4,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00593k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A lung tumor-on-a-chip model recapitulates the effect of hypoxia on radiotherapy response and FDG-PET imaging","authors":"Rohollah Nasiri, Myra Kurosu Jalil, Veronica Ibanez Gaspar, Andrea Sofia Flores Perez, Hieu Thi Minh Nguyen, Syamantak Khan, Sindy K. Y. Tang, Yunzhi Peter Yang and Guillem Pratx","doi":"10.1039/D5LC00373C","DOIUrl":"10.1039/D5LC00373C","url":null,"abstract":"<p >Most solid tumors contain regions of hypoxia that pose a significant challenge to the efficacy of radiation therapy. This study introduces a novel 3D lung tumor-on-a-chip (ToC) model designed to replicate the hypoxic tumor microenvironment <em>in vitro</em> while also providing a platform for clinically relevant interventions such as radiotherapy and positron emission tomography (PET) imaging. To simulate the heterogeneous oxygen distribution found in tumors, the ToC model incorporates an oxygen gradient achieved through a straightforward chemical oxygen scavenging system. A unique innovation of this device is the integration of a thin scintillator plate, which enables high-resolution radioluminescence microscopy imaging of tumor metabolism under hypoxia and normoxia conditions using clinically approved PET tracers such as fluorodeoxyglucose (FDG). The response of this hypoxic model to radiation therapy (10 Gy X-ray) demonstrated ∼4-fold higher radioresistance compared to the normoxic ToC model, as assessed by colony formation potential. Additionally, DNA damage observed in the normoxic ToC model was ∼5-fold higher than that in the hypoxic model. Furthermore, the metabolic consumption of glucose was found to mirror the localization of hypoxia, validating the use of this biomarker for planning radiation therapy. The integration of high-resolution radionuclide imaging within ToC models enables on-chip PET imaging and facilitates oncology research and discovery, offering innovative capabilities for the preclinical testing of novel cancer therapies in a clinically relevant environment.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 18","pages":" 4677-4691"},"PeriodicalIF":5.4,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Caplacizumab’s Potential to Mitigate Thrombosis Risk in Aortic Valve Stenosis: A Microfluidic and Computational Approach","authors":"Saeedreza Zeibi Shirejini, Mehrdad Khamooshi, Damien Riska, Martin Nikolov, Marjan Azimi, Shiyen L. Perera, Josie Carberry, Karen Alt, Shaun Gregory, Christoph Hagemeyer","doi":"10.1039/d5lc00361j","DOIUrl":"https://doi.org/10.1039/d5lc00361j","url":null,"abstract":"Aortic valve stenosis is a progressive cardiovascular disease associated with increased thrombotic risk due to abnormal blood flow patterns. Current management often culminates in valve replacement surgery, demonstrating the need for less invasive therapeutic options. This study investigates the potential of caplacizumab, a von Willebrand factor (vWF) inhibitor, in mitigating thrombosis risk in a microfluidic model of aortic valve stenosis. We employed a novel microfluidic model simulating the hemodynamics of healthy, moderate, and severe stenotic conditions, complemented by computational fluid dynamics simulations (CFD) and conventional platelet function assays. Microfluidic experiments revealed that shear gradients play a critical role in platelet aggregation, with accumulation intensifying as stenosis severity increased, even under constant peak shear rates. Caplacizumab demonstrated high specificity for vWF-mediated platelet activation, significantly inhibiting ristocetin-induced aggregation while not affecting ADP-induced aggregation. At an effective concentration (30 nM), caplacizumab reduced platelet coverage by up to 90% in high shear conditions (4500 s-1) and effectively mitigated shear gradient-dependent platelet aggregation across all stenotic conditions. These findings highlight caplacizumab's therapeutic potential for thrombosis prevention in patients with aortic valve stenosis, offering a foundation for personalized antithrombotic approaches that could potentially reduce thrombotic complications associated with the disease.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"20 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical-driven miniature robots: driving mechanism, applications and future trends","authors":"Xiaowen Wang, Sen Jia, Yingnan Gao, Changyou Liu, Yaping Wang, Anqin Liu and Wenguang Yang","doi":"10.1039/D5LC00621J","DOIUrl":"10.1039/D5LC00621J","url":null,"abstract":"<p >Miniature robots can complete complex tasks at the micro-scale, which have shown great application potential in fields such as biomedicine and environmental monitoring. As a renewable energy source, light is widely used in energy and information transmission. With the maturity of beam modulation and optical microscope technology, optical-driven miniature robots have become a hot topic in the field of miniature robotics due to their programmable nature, high resolution, non-contact nature, high precision, and good biocompatibility. This review introduces the driving mechanism of optical-driven miniature robots, summarizes the progress in their driving control and application capabilities, and looks forward to their future development trends. This review hopes to provide new ideas for the development of optical-driven miniature robots and promote their technological progress and applications. In the future, the cross-disciplinary integration of disciplines will help the research and development of the next generation of miniature robots.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 18","pages":" 4473-4507"},"PeriodicalIF":5.4,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144719785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}