Lab on a Chip最新文献_第10页

Electric field temporal interference stimulation of neurons in vitro† 体外神经元的电场时间干扰刺激。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-07-12 DOI: 10.1039/D4LC00224E

Annika Ahtiainen, Lilly Leydolph, Jarno M. A. Tanskanen, Alexander Hunold, Jens Haueisen and Jari A. K. Hyttinen

{"title":"Electric field temporal interference stimulation of neurons in vitro†","authors":"Annika Ahtiainen, Lilly Leydolph, Jarno M. A. Tanskanen, Alexander Hunold, Jens Haueisen and Jari A. K. Hyttinen","doi":"10.1039/D4LC00224E","DOIUrl":"10.1039/D4LC00224E","url":null,"abstract":"Electrical stimulation (ES) techniques, such as deep brain and transcranial electrical stimulation, have shown promise in alleviating the symptoms of depression and other neurological disorders in vivo. A new noninvasive ES method called temporal interference stimulation (TIS), possesses great potential as it can be used to steer the stimulation and possibly selectively modulate different brain regions. To study TIS in a controlled environment, we successfully established an in vitro ‘TIS on a chip’ setup using rat cortical neurons on microelectrode arrays (MEAs) in combination with a current stimulator. We validated the developed TIS system and demonstrated the spatial steerability of the stimulation by direct electric field measurements in the chip setup. We stimulated cultures of rat cortical neurons at 28 days in vitro (DIV) by two-channel stimulation delivering 1) TIS at 653 Hz and 643 Hz, resulting in a 10 Hz frequency envelope, 2) low-frequency stimulation (LFS) at 10 Hz and 3) high-frequency stimulation (HFS) at 653 Hz. Unstimulated cultures were used as control/sham. We observed the differences in the electric field strengths during TIS, HFS, and LFS. Moreover, HFS and LFS had the smallest effects on neuronal activity. Instead, TIS elicited neuronal electrophysiological responses, especially 24 hours after stimulation. Our ‘TIS on a chip’ approach eludicates the applicability of TIS as a method to modulate neuronal electrophysiological activity. The TIS on a chip approach provides spatially steerable stimuli while mitigating the effects of high stimulus fields near the stimulation electrodes. Thus, the approach opens new avenues for stimulation on a chip applications, allowing the study of neuronal responses to gain insights into the potential clinical applications of TIS in treating various brain disorders.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/lc/d4lc00224e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early detection of hypo/hyperglycemia using a microneedle electrode array-based biosensor for glucose ultrasensitive monitoring in interstitial fluid 使用基于微针电极阵列的生物传感器及早检测低血糖/高血糖，对组织间液中的葡萄糖进行超灵敏监测

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-07-10 DOI: 10.1039/D4LC00365A

Samar H. Tawakey, Mohammad Mansour, Ahmed Soltan and Alyaa I. Salim

{"title":"Early detection of hypo/hyperglycemia using a microneedle electrode array-based biosensor for glucose ultrasensitive monitoring in interstitial fluid","authors":"Samar H. Tawakey, Mohammad Mansour, Ahmed Soltan and Alyaa I. Salim","doi":"10.1039/D4LC00365A","DOIUrl":"10.1039/D4LC00365A","url":null,"abstract":"Diabetes is a common chronic metabolic disease with a wide range of clinical symptoms and consequences and one of the main causes of death. For the management of diabetes, painless and continuous interstitial fluid (ISF) glucose monitoring is ideal. Here, we demonstrate continuous diabetes monitoring using an integrated microneedle (MN) biosensor with an emergency alert system. MNs are a novel technique in the field of biomedical engineering because of their ability to analyze bioinformation with minimal invasion. In this work we developed a poly(methyl methacrylate) (PMMA) based MN glucose sensor. The device was produced by the 3D printing technique, microfabrication, electrodeposition, and enzyme immobilization step. The in vitro test for the glucose MN sensor showed a linear range from 1.5 to 14 mM with a sensitivity of 1.51 μA mM−1, limit of detection (LOD) of 0.35 mM and good selectivity. Highly repeatable sensing is observed with good reproducibility. The interference-free detection of glucose in the presence of physiologically relevant concentrations of ascorbic acid, uric acid, and mannose is demonstrated, along with the operational stability of the array. After resolving the biofouling consequences linked to on-body sensing, this MN platform would be appealing for minimally invasive electrochemical glucose monitoring. An alert is sent to confidants via email or SMS when the values are abnormal. The application is also able to display the recorded values in the form of a graph to help determine the state of health of the user over a period of time. It can be concluded that continuous monitoring and an emergency alert system are important for keeping an eye on diabetic patients and can send alert in case of an abnormal situation of the patient.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141566048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multioxide combinatorial libraries: fusing synthetic approaches and additive technologies for highly orthogonal electronic noses† 多氧化物组合文库：融合合成方法和添加技术，打造高度正交的电子鼻

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-07-08 DOI: 10.1039/D4LC00252K

Vishalkumar Rajeshbhai Gohel, Margarita Chetyrkina, Andrey Gaev, Nikolay P. Simonenko, Tatiana L. Simonenko, Philipp Yu. Gorobtsov, Nikita A. Fisenko, Darya A. Dudorova, Valeriy Zaytsev, Anna Lantsberg, Elizaveta P. Simonenko, Albert G. Nasibulin and Fedor S. Fedorov

{"title":"Multioxide combinatorial libraries: fusing synthetic approaches and additive technologies for highly orthogonal electronic noses†","authors":"Vishalkumar Rajeshbhai Gohel, Margarita Chetyrkina, Andrey Gaev, Nikolay P. Simonenko, Tatiana L. Simonenko, Philipp Yu. Gorobtsov, Nikita A. Fisenko, Darya A. Dudorova, Valeriy Zaytsev, Anna Lantsberg, Elizaveta P. Simonenko, Albert G. Nasibulin and Fedor S. Fedorov","doi":"10.1039/D4LC00252K","DOIUrl":"10.1039/D4LC00252K","url":null,"abstract":"This study evaluates the performance advancement of electronic noses, on-chip engineered multisensor systems, exploiting a combinatorial approach. We analyze a spectrum of metal oxide semiconductor materials produced by individual methods of liquid-phase synthesis and a combination of chemical deposition and sol–gel methods with hydrothermal treatment. These methods are demonstrated to enable obtaining a fairly wide range of nanomaterials that differ significantly in chemical composition, crystal structure, and morphological features. While synthesis routes foster diversity in material properties, microplotter printing ensures targeted precision in making on-chip arrays for evaluation of a combinatorial selectivity concept in the task of organic vapor, like alcohol homologs, acetone, and benzene, classification. The synthesized nanomaterials demonstrate a high chemiresistive response, with a limit of detection beyond ppm level. A specific combination of materials is demonstrated to be relevant when the number of sensors is low; however, such importance diminishes with an increase in the number of sensors. We show that on-chip material combinations could favor selectivity to a specific analyte, disregarding the others. Hence, modern synthesis methods and printing protocols supported by combinatorial analysis might pave the way for fabricating on-chip orthogonal multisensor systems.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A centrifugal-driven spiral microchannel microfiltration chip for emulsion and deformable particle sorting† 用于乳液和可变形颗粒分选的离心驱动螺旋微通道微过滤芯片。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-07-04 DOI: 10.1039/D4LC00260A

Yongchao Cai, Zekun Li, Cuimin Sun, Xuan Zhao, Shixiong Wu, Guangyong Huang, Shengchang Tang, Peng Dai, Xiangfu Wei and Hui You

{"title":"A centrifugal-driven spiral microchannel microfiltration chip for emulsion and deformable particle sorting†","authors":"Yongchao Cai, Zekun Li, Cuimin Sun, Xuan Zhao, Shixiong Wu, Guangyong Huang, Shengchang Tang, Peng Dai, Xiangfu Wei and Hui You","doi":"10.1039/D4LC00260A","DOIUrl":"10.1039/D4LC00260A","url":null,"abstract":"Droplet sorting and enrichment, as a prominent field within microfluidic technology, represent a pivotal stage in the manipulation of droplets and particles. In recent times, droplet sorting methods based on lab-on-disk (LOD) have garnered significant interest among researchers for their inherent merits, including high throughput, ease of operation, seamless device integration, and independence from supplementary driving forces. This study introduces a centrifugal force-driven microfluidic chip comprising spiral microchannels. The chip incorporates microhole arrays along the sidewall of the spiral channels, enabling size-based sorting and enrichment of microdroplets under the influence of multiple forces. Firstly, a comparative analysis was performed to assess the influence of the separation port structure and rotational speed on efficiency, and a mechanical modeling approach was employed to conduct kinetic analyses of droplet behavior during instantaneous separation. Those findings demonstrated a good agreement with the experimental results at ω < 100 rpm. Subsequently, sorting experiments on homogeneous droplets indicated that repetitive sorting could increase the recovery ratios, RT(α), of high-concentration droplets (20.7%) from 35.3% to over 80%. We also conducted a sorting experiment on three-component homogeneous-phase emulsions using a serially connected chip array, and the sorting throughput was 0.58 mL min−1. As a result, the RT(α) for 60 and 160 μm droplets were 99.4% and 88.9%, respectively. Lastly, we conducted elution experiments and dual-sample sorting on a single chip, and the fluorescence results demonstrated that this study provided an efficient and non-cross-contaminating sorting method for non-homogenous phase multi-sample microreactor units.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141546238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modelling the innate immune system in microphysiological systems 在微物理系统中模拟先天性免疫系统。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-07-03 DOI: 10.1039/D3LC00812F

Michael J. Rupar, Hannah Hanson, Stephanie Rogers, Brianna Botlick, Steven Trimmer and James J. Hickman

引用次数: 0

Microreactor designed for efficient plasma–liquid segmented flows† 专为高效等离子体-液体分段流设计的微反应器

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-07-02 DOI: 10.1039/D4LC00315B

Pierre Dedieu, Gabriel Morand, Karine Loubière, Stéphanie Ognier and Michael Tatoulian

{"title":"Microreactor designed for efficient plasma–liquid segmented flows†","authors":"Pierre Dedieu, Gabriel Morand, Karine Loubière, Stéphanie Ognier and Michael Tatoulian","doi":"10.1039/D4LC00315B","DOIUrl":"10.1039/D4LC00315B","url":null,"abstract":"Microreactors were designed for gas–liquid plasma chemical processes and operated under segmented flows in a high aspect ratio (8.76) rectangular microchannel. First, the hydrodynamics of the gas–liquid flows generated at a T-junction was investigated for fifteen solvents commonly used in organic synthesis. The classical literature scaling laws were revised to describe the dependence of bubble and slug lengths, and bubble residence time on the liquid nature by introducing their liquid vapour pressure. Liquid film thickness and liquid residence time were estimated from residence time distribution experiments. Secondly, plasma could be successfully generated in these segmented flows for all the liquids. Due to the plasma dissipation of thermal energy, gas phase temperature increased and induced the lengthening of bubbles and the decrease in bubble residence time. The flow pattern was also impacted by the gas temperature increase. A flow map describing the evolution of the flow pattern under plasma conditions was built, enabling prediction of the flow pattern based on the liquid boiling point and dielectric constant. These microreactors have demonstrated great potential, and by adapting the synthesis solvent or the operating plasma conditions, they could find promising applications in gas–liquid plasma chemical processes.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141489489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic finger-actuated mixer for ultrasensitive electrochemical measurements of protein biomarkers for point-of-care testing† 用于超灵敏电化学测量蛋白质生物标记物的微流控指动混合器，可用于床旁检测

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-07-02 DOI: 10.1039/D4LC00207E

Benjamin Utzinger, Desh Deepak Dixit and Peter B. Lillehoj

{"title":"Microfluidic finger-actuated mixer for ultrasensitive electrochemical measurements of protein biomarkers for point-of-care testing†","authors":"Benjamin Utzinger, Desh Deepak Dixit and Peter B. Lillehoj","doi":"10.1039/D4LC00207E","DOIUrl":"10.1039/D4LC00207E","url":null,"abstract":"Current diagnostic tests for high sensitivity detection of protein biomarkers involve long incubation times or require bulky/expensive instrumentation, hindering their use for point-of-care testing. Here, we report a microfluidic electrochemical immunosensor that employs a unique finger-actuated mixer for rapid, ultrasensitive measurements of protein biomarkers. Mixing was implemented during the incubation steps, which accelerated biomolecular transport and promoted immunocomplex formation, leading to enhanced analytical sensitivity and a shortened detection time. Electrochemical measurements were performed using a handheld diagnostic device consisting of a smartphone and miniature potentiostat. Proof of principle was demonstrated by using this platform for quantitative measurements of C–X–C motif chemokine ligand 9 (CXCL9), a serological biomarker for autoimmune and inflammatory diseases, which could be detected in human plasma at concentrations as low as 4.7 pg mL−1 in <25 min. The ability to rapidly detect protein biomarkers with high sensitivity in a point-of-care format makes this device a promising tool for diagnostic testing, particularly in resource-limited settings.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141495611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sample preparation and detection methods in point-of-care devices towards future at-home testing 面向未来家庭检测的护理点设备中的样品制备和检测方法。

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-07-02 DOI: 10.1039/D3LC00943B

George Adedokun, Morteza Alipanah and Z. Hugh Fan

引用次数: 0

Deformation under flow and morphological recovery of cancer cells† 癌细胞的流动变形和形态恢复

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-07-01 DOI: 10.1039/D4LC00246F

Emile Gasser, Emilie Su, Kotryna Vaidžiulytė, Nassiba Abbade, Hamizah Cognart, Jean-Baptiste Manneville, Jean-Louis Viovy, Matthieu Piel, Jean-Yves Pierga, Kyohei Terao and Catherine Villard

{"title":"Deformation under flow and morphological recovery of cancer cells†","authors":"Emile Gasser, Emilie Su, Kotryna Vaidžiulytė, Nassiba Abbade, Hamizah Cognart, Jean-Baptiste Manneville, Jean-Louis Viovy, Matthieu Piel, Jean-Yves Pierga, Kyohei Terao and Catherine Villard","doi":"10.1039/D4LC00246F","DOIUrl":"10.1039/D4LC00246F","url":null,"abstract":"The metastatic cascade includes a blood circulation step for cells detached from the primary tumor. This stage involves significant shear stress as well as large and fast deformation as the cells circulate through the microvasculature. These mechanical stimuli are well reproduced in microfluidic devices. However, the recovery dynamics after deformation is also pivotal to understand how a cell can pass through the multiple capillary constrictions encountered during a single hemodynamic cycle. The microfluidic system developed in this work allows single cell recovery to be studied under flow-free conditions following pressure-actuated cell deformation inside constricted microchannels. We used three breast cancer cell lines – namely MCF-7, SK-BR3 and MDA-MB231 – as cellular models representative of different cancer phenotypes. Changing the size of the constriction allows exploration of moderate to strong deformation regimes, the latter being associated with the formation of plasma membrane blebs. In the regime of moderate deformation, all cell types display a fast elastic recovery behavior followed by a slower viscoelastic regime, well described by a double exponential decay. Among the three cell types, cells of the mesenchymal phenotype, i.e. the MDA-MB231 cells, are softer and the most fluid-like, in agreement with previous studies. Our main finding here is that the fast elastic recovery regime revealed by our novel microfluidic system is under the control of cell contractility ensured by the integrity of the cell cortex. Our results suggest that the cell cortex plays a major role in the transit of circulating tumor cells by allowing their fast morphological recovery after deformation in blood capillaries.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/lc/d4lc00246f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141475185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urine osmolality assessment through the integration of urea hydrolysis and impedance measurement† 通过尿素水解与阻抗测量相结合评估尿液渗透压

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2024-07-01 DOI: 10.1039/D4LC00114A

Tian Fook Kong, Xinhui Shen, Mei Yi Sim, Jin Yong, Tze Kiat Ng, Tsung Wen Chong and Marcos

{"title":"Urine osmolality assessment through the integration of urea hydrolysis and impedance measurement†","authors":"Tian Fook Kong, Xinhui Shen, Mei Yi Sim, Jin Yong, Tze Kiat Ng, Tsung Wen Chong and Marcos","doi":"10.1039/D4LC00114A","DOIUrl":"10.1039/D4LC00114A","url":null,"abstract":"We present the development and validation of an impedance-based urine osmometer for accurate and portable measurement of urine osmolality. The urine osmolality of a urine sample can be estimated by determining the concentrations of the conductive solutes and urea, which make up approximately 94% of the urine composition. Our method utilizes impedance measurements to determine the conductive solutes and urea after hydrolysis with urease enzyme. We built an impedance model using sodium chloride (NaCl) and urea at various known concentrations. In this work, we validated the accuracy of the impedance-based urine osmometer by developing a proof-of-concept first prototype and an integrated urine dipstick second prototype, where both prototypes exhibit an average accuracy of 95.5 ± 2.4% and 89.9 ± 9.1%, respectively in comparison to a clinical freezing point osmometer in the hospital laboratory. While the integrated dipstick design exhibited a slightly lower accuracy than the first prototype, it eliminated the need for pre-mixing or manual pipetting. Impedance calibration curves for conductive and non-conductive solutes consistently yielded results for NaCl but underscored challenges in achieving uniform urease enzyme coating on the dipstick. We also investigated the impact of storing urine at room temperature for 24 hours, demonstrating negligible differences in osmolality values. Overall, our impedance-based urine osmometer presents a promising tool for point-of-care urine osmolality measurements, addressing the demand for a portable, accurate, and user-friendly device with potential applications in clinical and home settings.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/lc/d4lc00114a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141475241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0