Lab on a Chip最新文献_第9页

Correction: Evaluating migration and cytotoxicity of tissue-resident and conventional NK cells in a 3D microphysiological system using live-cell imaging 更正：使用活细胞成像技术在三维微生理系统中评估组织驻留和常规NK细胞的迁移和细胞毒性

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-08-11 DOI: 10.1039/D5LC90087E

Hyeri Choi, June Ho Shin, Hyeonsu Jo, John B. Sunwoo and Noo Li Jeon

引用次数: 0

High-speed cell partitioning through reactive machine learning-guided inkjet printing 通过反应性机器学习引导的喷墨打印实现高速单元划分

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-08-08 DOI: 10.1039/D5LC00514K

Eric Cheng, Glenn Chang, Haley MacDonald, Miguel Ramirez, Pamela A. Hoodless, Robin Coope, Adi Steif and Karen C. Cheung

{"title":"High-speed cell partitioning through reactive machine learning-guided inkjet printing","authors":"Eric Cheng, Glenn Chang, Haley MacDonald, Miguel Ramirez, Pamela A. Hoodless, Robin Coope, Adi Steif and Karen C. Cheung","doi":"10.1039/D5LC00514K","DOIUrl":"10.1039/D5LC00514K","url":null,"abstract":"Partitioning cells in open nanowells permits high confidence in single cell occupancy and enables flexibility in the development of different molecular assays. A challenge for this approach however is to print cells sufficiently quickly to enable experiments of adequate statistical power in a reasonable time. To address this, we developed a single cell dispensing instrument leveraging inkjet technology with continuous real-time optical feedback and machine learning algorithms for high-throughput single cell isolation. The Isolatrix enables rapid partitioning of cells into open substrates such as nanowell arrays, permitting high-throughput application of custom genomic assays such as direct-transposition single cell whole genome sequencing (scWGS). We trained the classifier on manually labelled data with a range of cell sizes and applied the instrument to generate scWGS profiles from cell lines and primary mouse tissue. Comparison to existing predictive workflows demonstrated that this reactive approach, featuring machine learning classification of events post-dispensing, gives up to a 9.69 times increase in isolation speed. Validation via fluorescent imaging of cell lines confirmed a classification accuracy of 98.7%, at a rate of 0.52 seconds per single cell, under tuned spotting parameters. Genomic analysis showed low background contamination and high coverage uniformity across the genome, enabling detection of chromosomal copy number alterations. With data tracing capabilities and a convenient user interface, we expect the Isolatrix to enable large-scale profiling of a range of genomic data modalities.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 19","pages":" 4972-4985"},"PeriodicalIF":5.4,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A compact automated magnetic digital microfluidic chemiluminescence immunoassay system for rapid and sensitive detection of protein biomarkers 一种紧凑的自动磁数字微流体化学发光免疫分析系统，用于快速灵敏地检测蛋白质生物标志物

IF 6.1 2区工程技术

Lab on a Chip Pub Date : 2025-08-07 DOI: 10.1039/d5lc00496a

Chuan Lyu, Dian Yang, Xu Xu, Yu Cai, Bo Liang, Congcong Zhou, Xuesong Ye, Jing Wang

{"title":"A compact automated magnetic digital microfluidic chemiluminescence immunoassay system for rapid and sensitive detection of protein biomarkers","authors":"Chuan Lyu, Dian Yang, Xu Xu, Yu Cai, Bo Liang, Congcong Zhou, Xuesong Ye, Jing Wang","doi":"10.1039/d5lc00496a","DOIUrl":"https://doi.org/10.1039/d5lc00496a","url":null,"abstract":"The rapid and sensitive detection of specific protein biomarkers is essential for early diagnosis and monitoring of diseases. Magnetic digital microfluidics (MDMF), capable of integrating and miniaturizing chemiluminescence immunoassay (CLIA), offers significant advantages for biomarker detection, including high flexibility, high efficiency, and low sample consumption. Magnetic bead manipulation, as a key component of MDMF, plays a critical role in improving detection sensitivity and accuracy within a short analysis time. In this study, we proposed an automated MDMF-based CLIA system and developed a compact analyzer, enabling faster and more sensitive biomarker detection through optimized magnetic bead manipulation. The system employs a film-sandwiched microfluidic chip, along with a specially designed spliced magnet to generate a stronger and more concentrated magnetic force, significantly enhancing the control over the motion of magnetic beads. By using the magnet to drive magnetic beads through water-in-oil droplets, the system automates the entire CLIA workflow. Through theoretical analysis, simulations, and experiments, we validate the system's advantages in magnetic bead aggregation and transfer, achieving a high magnetic bead collection rate of 97.07% with excellent cleaning effect after three cleaning rounds. The detection of cardiac troponin I (cTnI) can be completed within 10 min, with a limit of detection (LOD) of 2.09 pg/mL, a detection range of 0.01 to 20 ng/mL, and a strong linear correlation with conventional CLIA (R² = 0.9961). This system achieves rapid, sensitive, and automated CLIA, making it a promising platform for clinical diagnostics.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"12 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fingertip-chip sensor based on Pd nanocluster sensitized 3D NiO nanotube arrays for real-time, selective methane detection 基于Pd纳米簇敏化三维NiO纳米管阵列的指尖芯片传感器用于实时、选择性甲烷检测

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-08-07 DOI: 10.1039/D5LC00570A

Kunmei Yang, Yue Kang, Jia Yan, Weihao Fang, Jiazhen Zhang and Zhilong Song

{"title":"Fingertip-chip sensor based on Pd nanocluster sensitized 3D NiO nanotube arrays for real-time, selective methane detection","authors":"Kunmei Yang, Yue Kang, Jia Yan, Weihao Fang, Jiazhen Zhang and Zhilong Song","doi":"10.1039/D5LC00570A","DOIUrl":"10.1039/D5LC00570A","url":null,"abstract":"The selective detection of methane (CH4) at trace levels is essential for applications such as mining safety and natural gas leak detection. However, achieving high selectivity and sensitivity remains a significant challenge due to interference from gases like hydrogen sulfide (H2S) and carbon monoxide (CO). In this study, we present a novel fingertip-chip sensor that combines palladium (Pd) nanoclusters with three-dimensional (3D) nickel oxide (NiO) nanotube arrays for highly selective and sensitive CH4 detection. The 3D NiO structure offers a large surface area that enhances CH4 adsorption, while the Pd nanoclusters serve as catalytic sites, improving the interaction between CH4 molecules and the NiO surface. Fabricated via atomic layer deposition (ALD), the sensor demonstrates an ultra-low detection limit of 70 parts per billion (ppb) and exceptional selectivity, with a response ratio greater than 10 for CH4 relative to common interferents such as H2S and CO. Comprehensive evaluations of the sensor's sensitivity, stability, and performance under varying environmental conditions confirm its potential for real-time monitoring. Integrated into a wireless fingertip-chip system, the sensor enables seamless, remote CH4 monitoring in dynamic and challenging environments, such as mining sites and natural gas pipelines. This work presents a scalable approach for next-generation safety gas sensors, enhancing both detection sensitivity and real-time applicability in industrial and environmental monitoring.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 19","pages":" 4986-4993"},"PeriodicalIF":5.4,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144792814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A compact sample-to-answer system for rapid MRSA detection in serum based on reagent-free electrophoretic purification of nucleic acids and colorimetric LAMP 一种基于核酸无试剂电泳纯化和LAMP比色法的血清MRSA快速检测系统

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-08-06 DOI: 10.1039/D5LC00152H

Yung Ching Lee, Yang Bu, Sheng Ni, Yuze Liu, Anni Hu and Levent Yobas

{"title":"A compact sample-to-answer system for rapid MRSA detection in serum based on reagent-free electrophoretic purification of nucleic acids and colorimetric LAMP","authors":"Yung Ching Lee, Yang Bu, Sheng Ni, Yuze Liu, Anni Hu and Levent Yobas","doi":"10.1039/D5LC00152H","DOIUrl":"10.1039/D5LC00152H","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant threat as a leading cause of nosocomial infections, inflicting severe complications and fatalities worldwide. Its rising prevalence has become a major public health concern as its resistance to common antibiotics complicates treatments, placing additional burden on healthcare systems. Microbial culture is the “gold standard” for diagnosing MRSA; however, this method is time-consuming and labor-intensive, often leading to prolonged delays in diagnosis and treatment. In contrast, nucleic acid amplification tests (NAATs) dramatically reduce diagnostic times to mere hours, while maintaining high sensitivity and specificity. Bringing NAATs to the point of care can facilitate timely treatment decisions and yet requires a compact “sample-to-answer” system whose development has long been hindered by the required sample preparation for these tests. Here, we present such a system detecting MRSA in human serum through a simple microfluidic chip, achieving a limit of detection of 1 CFU per reaction and a turnaround time of just 45 min. The chip effectively overcomes the sample preparation challenge with an innovative use of a sieve, a dense array of micropillars with submicrometer gaps. Along with associated reservoirs, this sieve integrates bacterial lysis, reagent-free electrophoretic purification and loop-mediated isothermal amplification (LAMP) of nucleic acids with colorimetric detection visible to the naked eye. Within the sieve, nucleic acids are selectively driven by rotating electric fields and focused near the sieve center while steady electric fields remove all contaminants, without the need for reagents. The system shows great potential for point-of-care diagnostics.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 19","pages":" 5019-5029"},"PeriodicalIF":5.4,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00152h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144787338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Groove-aided sacrificial molding for fabrication of an in vitro vascular model with branches using ECM-derived materials 利用ecm衍生材料制造具有分支的体外血管模型的凹槽辅助牺牲模塑

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-08-06 DOI: 10.1039/D5LC00214A

Jumpei Muramatsu, Michinao Hashimoto, Shigenori Miura and Hiroaki Onoe

{"title":"Groove-aided sacrificial molding for fabrication of an in vitro vascular model with branches using ECM-derived materials","authors":"Jumpei Muramatsu, Michinao Hashimoto, Shigenori Miura and Hiroaki Onoe","doi":"10.1039/D5LC00214A","DOIUrl":"10.1039/D5LC00214A","url":null,"abstract":"This paper describes a fabrication method of an in vitro branched vascular model using extracellular matrix (ECM)-derived materials (transglutaminase crosslinked gelatin, TG-gelatin). Mechanical stresses, such as disturbed blood flow derived from vascular branches, are a significant cause of cardiovascular disease. To study cardiovascular diseases, a perfusion and stretching culture platform with an ECM-based in vitro vascular model with branches has been essential. Among the proposed microchannel fabrication methods, sacrificial molding with a template made of soluble material is attractive for fabricating branched microchannels. However, the soluble template remained an issue of undesired deformation due to the swelling caused by the moisture in the hydrogel. Here, we propose groove-aided sacrificial molding (GAS molding) to suppress the deformation of the soluble template in the TG-gelatin. By preparing a channel-shaped groove on the TG-gelatin, the shape retention of the soluble template is assisted. We experimentally evaluated the deformation of the microchannels fabricated by the proposed GAS molding. The vascular endothelial cells were seeded into microchannels fabricated by GAS molding for perfusion and stretching culture. The effects of mechanical stress were visualized by immunofluorescence staining of PECAM1 and integrin α9 in the endothelial cells. Overall, our method would suggest a platform to spatially elucidate the cellular responses to irregular mechanical stresses, such as triggers of cardiovascular disease.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 18","pages":" 4800-4813"},"PeriodicalIF":5.4,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00214a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144787339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Pipette-operable microfluidic devices with hydrophobic valves in sequential dispensing with various liquid samples: multiplex disease assay by RT-LAMP 校正：带疏水阀的移液管可操作微流体装置，用于各种液体样品的顺序配药；RT-LAMP多重疾病检测

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-08-05 DOI: 10.1039/D5LC90081F

Yen-Wei Chang, Jhih-Pu Lin, Shiu-Jie Ling, Yen-Chun Chen, Helene Minyi Liu and Yen-Wen Lu

引用次数: 0

Simple additive-based modifications of PDMS for long-term hydrophilic stability† 基于添加剂的PDMS长期亲水稳定性的简单改性。

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-08-05 DOI: 10.1039/D5LC00532A

Eunyoung Park, Seungjin Kang and Ung Hyun Ko

{"title":"Simple additive-based modifications of PDMS for long-term hydrophilic stability†","authors":"Eunyoung Park, Seungjin Kang and Ung Hyun Ko","doi":"10.1039/D5LC00532A","DOIUrl":"10.1039/D5LC00532A","url":null,"abstract":"Polydimethylsiloxane (PDMS) is widely used in microfluidic systems due to its ease of fabrication and versatile properties; however, its inherent hydrophobicity limits its utility. In this study, we fabricated hydrophilic PDMS in a one-step process by incorporating a PDMS–poly(ethylene glycol) block copolymer into two commercially available PDMS formulations (Sylgard 184 and KE-106), requiring only mixing, without additional steps such as plasma treatment or chemical coating. The contact angles of the modified PDMS and the surrounding glass surfaces assembled with PDMS gradually increased over the assembled period, but the overall hydrophilic properties were retained for up to two months, enabling stable fluid flow within microfluidic channels as small as 3 μm in height. Interestingly, our hydrophilic PDMS exhibited dynamic wettability transition, which varied between the two PDMS formulations, with KE-106 showing a faster and more extensive hydrophilic transformation compared to Sylgard 184. Gas chromatography-mass spectrometry analysis confirmed a higher release of hydrophobic PDMS compounds, namely D4 and D5, from Sylgard 184 compared to KE-106. Based on these results, we suggest that differences in PDMS compounds affect the efficiency and performance of the block copolymer-mediated hydrophilization of PDMS. These findings provide crucial insights into designing hydrophilic PDMS-based microfluidic devices, particularly for long-term biomedical applications requiring reliable fluid flow and hemocompatibility.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 18","pages":" 4776-4786"},"PeriodicalIF":5.4,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144777902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Orbital shaker-driven gut-on-a-chip platform for drug-induced permeability and microenvironment studies 轨道激振器驱动的芯片肠道药物诱导通透性和微环境研究平台

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-08-05 DOI: 10.1039/D5LC00333D

Nishanth Venugopal Menon, Jeeyeon Lee, Hung Dong Truong, Sriram Bharathkumar and Chwee Teck Lim

{"title":"Orbital shaker-driven gut-on-a-chip platform for drug-induced permeability and microenvironment studies","authors":"Nishanth Venugopal Menon, Jeeyeon Lee, Hung Dong Truong, Sriram Bharathkumar and Chwee Teck Lim","doi":"10.1039/D5LC00333D","DOIUrl":"10.1039/D5LC00333D","url":null,"abstract":"Gut-on-a-chip platforms replicate realistic gut microenvironments but face limited adoption due to their complex designs, expensive fabrication, and specialized instrumentation that increases operational complexity. In this study, we present a microfluidic chip insertable into 12-well plates with a unique radial design and a pumpless flow actuation system using an orbital shaker. We use a surface tension-driven hydrogel patterning technique to compartmentalize the chip, enabling co-culture of gut epithelium and vasculature, resulting in leak-proof monolayer tubes. Furthermore, computational fluid dynamic analysis demonstrates bidirectional peristaltic flow induced by the shaker. The platform's physiological relevance is confirmed through the evaluation of cell polarization, tight junction markers and barrier integrity, using high-magnification microscopy and electrical resistance measurements. We also demonstrate the ability of the platform to support live bacterial colonization, simulating host–microbe interactions. The model is validated for drug development by assessing gut and vascular permeability following drug overdose and inflammatory cytokine activation. Additionally, we explore nanoplastic poisoning using nano polyethylene terephthalate (PET) particles, highlighting the gut's role in limiting particle absorption into the bloodstream. The orbital gut-on-a-chip platform offers an accessible, dynamic cell culture system for drug discovery and biomimetic modeling of gut-related disease interactions.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 19","pages":" 5005-5018"},"PeriodicalIF":5.4,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144787080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidics for geosciences: metrological developments and future challenges 地球科学的微流体：计量学的发展和未来的挑战。

IF 5.4 2区工程技术

Lab on a Chip Pub Date : 2025-08-04 DOI: 10.1039/D5LC00108K

Sophie Roman, Flore Rembert, Anthony R. Kovscek and Jenna Poonoosamy

{"title":"Microfluidics for geosciences: metrological developments and future challenges","authors":"Sophie Roman, Flore Rembert, Anthony R. Kovscek and Jenna Poonoosamy","doi":"10.1039/D5LC00108K","DOIUrl":"10.1039/D5LC00108K","url":null,"abstract":"This review addresses the main metrological developments over the past decade for microfluidics applied to geosciences. Microfluidic experiments for geosciences seek to decipher the complex interplay between coupled, multiphase, and reactive processes in geological porous media, e.g., for groundwater management, soil remediation, gas storage in geological reservoirs, or geothermal energy. The guiding principle is to represent natural or engineered processes in a controlled environment to observe, characterize, and model them. When microfluidic experiments are associated with advanced metrology techniques, they provide direct visualization of the processes and measurements of transport mechanisms, chemical reactions, interfacial processes, or mixing within the pore space. In this review, we present the state of the art in metrological approaches to microfluidics for geosciences, including measuring velocity fields, fluid and solute saturations, tracking chemical reactions, and combining experimental and computational microfluidics. The upscaling from microfluidics to the reservoir scale is discussed. Finally, we outline future challenges related to metrological advancements and the integration of artificial intelligence in microfluidics.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 17","pages":" 4273-4289"},"PeriodicalIF":5.4,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00108k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0