Lab on a Chip最新文献

{"title":"Cancer-on-a-chip for precision cancer medicine","authors":"Lunan Liu, Huishu Wang, Ruiqi Chen, Yujing Song, William Wei, David Baek, Mahan Gillin, Katsuo Kurabayashi and Weiqiang Chen","doi":"10.1039/D4LC01043D","DOIUrl":"10.1039/D4LC01043D","url":null,"abstract":"<p >Many cancer therapies fail in clinical trials despite showing potent efficacy in preclinical studies. One of the key reasons is the adopted preclinical models cannot recapitulate the complex tumor microenvironment (TME) and reflect the heterogeneity and patient specificity in human cancer. Cancer-on-a-chip (CoC) microphysiological systems can closely mimic the complex anatomical features and microenvironment interactions in an actual tumor, enabling more accurate disease modeling and therapy testing. This review article concisely summarizes and highlights the state-of-the-art progresses in CoC development for modeling critical TME compartments including the tumor vasculature, stromal and immune niche, as well as its applications in therapying screening. Current dilemma in cancer therapy development demonstrates that future preclinical models should reflect patient specific pathophysiology and heterogeneity with high accuracy and enable high-throughput screening for anticancer drug discovery and development. Therefore, CoC should be evolved as well. We explore future directions and discuss the pathway to develop the next generation of CoC models for precision cancer medicine, such as patient-derived chip, organoids-on-a-chip, and multi-organs-on-a-chip with high fidelity. We also discuss how the integration of sensors and microenvironmental control modules can provide a more comprehensive investigation of disease mechanisms and therapies. Next, we outline the roadmap of future standardization and translation of CoC technology toward real-world applications in pharmaceutical development and clinical settings for precision cancer medicine and the practical challenges and ethical concerns. Finally, we overview how applying advanced artificial intelligence tools and computational models could exploit CoC-derived data and augment the analytical ability of CoC.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 14","pages":" 3314-3347"},"PeriodicalIF":6.1,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roll-to-roll fabrication of three-dimensional self-folding microstructures†","authors":"Tongyao Wu, Ali Mehrnezhad and Kidong Park","doi":"10.1039/D5LC00120J","DOIUrl":"https://doi.org/10.1039/D5LC00120J","url":null,"abstract":"<p >Self-folding technology offers a promising alternative to conventional microfabrication techniques. It utilizes controlled and imbalanced stresses to transform specific patterns of flat materials into pre-determined three-dimensional (3D) structures for diverse applications. However, current production methods of self-folding structures are mostly limited to lab-scale production. In this study, a novel roll-to-roll (R2R) production setup is developed to address the limited scalability of self-folding technology. The R2R setup continuously stretches and bonds a pre-cured PDMS (polydimethylsiloxane) film to another PDMS film attached to a stiff PET (polyethylene terephthalate) carrier layer. This creates a bilayer PDMS film with imbalanced stress, causing it to self-fold into pre-determined 3D shapes upon patterning and releasing from the PET carrier layer. The R2R setup achieves a production rate of 96 cm<small>²</small> min<small>⁻¹</small>, significantly surpassing our previous method based on spin-coating and baking. This demonstrates the potential of R2R technology for industrial-scale production of self-folding microstructures.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 10","pages":" 2410-2418"},"PeriodicalIF":6.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hybrid dielectrophoretic trap–optical tweezers platform for manipulating microparticles in aqueous suspension†","authors":"Carlos David González-Gómez, Jose Garcia-Guirado, Romain Quidant, Félix Carrique, Emilio Ruiz-Reina and Raúl A. Rica-Alarcón","doi":"10.1039/D4LC00982G","DOIUrl":"10.1039/D4LC00982G","url":null,"abstract":"<p >We demonstrate that a set of microfabricated electrodes can be coupled to a commercial optical tweezers device, implementing a hybrid electro-optical platform with multiple functionalities for the manipulation of micro-/nanoparticles in suspension. We show that the hybrid scheme allows enhanced manipulation capabilities, including hybrid dynamics, controlled accumulation in the dielectrophoretic trap from the optical tweezers, selectivity, and video tracking of the individual trajectories of trapped particles. This creates opportunities for novel studies in statistical physics and stochastic thermodynamics with multi-particle systems, previously limited to investigations with individual particles.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 10","pages":" 2462-2474"},"PeriodicalIF":6.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc00982g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel microfluidic self-perfusion chip (MSPC) for pumpless 3D cell, microtissue and organoid culture","authors":"Guohua Wu, Di Wu, Wenqi Hu, Qinrui Lu, Yusen Zhou, Jie Liu, Qijun Du, Zhi Luo, Haijie Hu, Hongwei Jiang, Bangchuan Hu and Shuqi Wang","doi":"10.1039/D5LC00030K","DOIUrl":"10.1039/D5LC00030K","url":null,"abstract":"<p >Microfluidic systems have revolutionized biological research by enabling precise control over cellular environments at microscale volumes. However, traditional pump-driven systems face challenges such as complexity, cost, cell-damaging shear stress, and limited portability. This study introduces a novel adjustable microfluidic self-perfusion chip (MSPC) that uses evaporation as a driving force, eliminating the need for external pumps. Our design offers improved metabolic waste management and simplified control over fluid dynamics. The chip features adjustable evaporation pore sizes, demonstrating a robust linear relationship (<em>R</em><small>²</small> = 0.95) between the pore size and fluid evaporation rate. This ensures consistent fluid flow and effective waste removal, shown by lower ammonia and lactate levels compared to conventional cultures. Its unidirectional flow system and integrated one-way valve maintain cell viability, even under complete evaporation conditions. This innovative platform facilitates the cultivation of complex tissue-like structures, providing a valuable tool for tissue and organ model development, as well as drug screening and toxicity testing. By addressing key limitations of traditional systems, our adjustable MSPC represents a significant advancement in microfluidic cell culture technology, offering improved accessibility and applicability in biological research.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 15","pages":" 3694-3706"},"PeriodicalIF":6.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic osmotic compression with operando meso-structure characterization using SAXS†","authors":"Dimitri Radajewski, Pierre Roblin, Patrice Bacchin, Martine Meireles and Yannick Hallez","doi":"10.1039/D4LC01087F","DOIUrl":"10.1039/D4LC01087F","url":null,"abstract":"<p >We have developed a microfluidic chip for the osmotic compression of samples at the nanoliter scale, enabling the <em>in situ</em> and <em>operando</em> acquisition of structural features through small-angle X-ray scattering throughout the compression process. The design builds upon a previous setup allowing high-throughput measurements with minimal sample quantities. The updated design is specifically tailored for compatibility with a laboratory beamline, taking into account factors such as reduced photon flux and increased beam size compared to synchrotron beamlines. As a proof of concept, we performed on-chip compression of well-documented silica colloidal particles (Ludox TM-50). We demonstrated that the volume fraction could be tracked over time during compression, either by monitoring X-ray absorbance or by modeling the scattered signal. With precise control of the osmotic pressure and salt chemical potential, equations of state can be determined unambiguously from the volume fraction measurements and be interpreted with the help of the scattered intensity. These microfluidic chips will be valuable for understanding the behavior of colloidal suspensions, with applications in areas such as crystallization, nucleation, soil mechanics, control of living matter growth and interaction conditions, as well as the measurement of coarse-grained colloidal interaction potentials.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 12","pages":" 2851-2861"},"PeriodicalIF":6.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d4lc01087f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Mechanical forces and enzymatic digestion act together to induce the remodeling of collagen fibrils in tumor microenvironment","authors":"Jiling Shi, Aihua Jing, Qinan Yin, Xuewei Zheng, Zhigang Hu, Xibin Jiao, Yaomin Fan, Xiangyang Zu, Jinghua Li, Yanping Liu, Jiayu Zhai, Xiucheng Li and Kena Song","doi":"10.1039/D5LC90037A","DOIUrl":"10.1039/D5LC90037A","url":null,"abstract":"<p >Correction for ‘Mechanical forces and enzymatic digestion act together to induce the remodeling of collagen fibrils in tumor microenvironment’ by Jiling Shi <em>et al.</em>, <em>Lab Chip</em>, 2025, https://doi.org/10.1039/d4lc00821a.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 10","pages":" 2491-2491"},"PeriodicalIF":6.1,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc90037a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of multi-coil shim and RF microstrip coils for high-resolution microfluidic nuclear magnetic resonance detection†","authors":"Huijun Sun, Xin Xie, Xinchang Wang, Yaohong Wang, Zhenggang Li, Junyao Xie, Kaiwen Yao, Jinglong Guan, Ying He and Zhong Chen","doi":"10.1039/D5LC00209E","DOIUrl":"10.1039/D5LC00209E","url":null,"abstract":"<p >The integration of nuclear magnetic resonance (NMR) and microfluidic technology provides an excellent detection method for detecting nanoscale micro-samples and analysing intermediates during <em>in situ</em> reaction processes. However, the non-cylindrical symmetric structure of microfluidic chips and micro-coils, along with magnetic susceptibility mismatches, results in a complex distorted magnetic field and reduces spectral resolution. Traditional spherical harmonic shimming methods and coils are global in nature for the target area, forming field distributions with a symmetrical form about the origin. This has significant limitations for shimming local distortions in non-origin-symmetric fields. In this paper, we propose a novel integrated probe suitable for high-resolution detection of microfluidic NMR, as well as a shimming method for local distortions. Specifically: (1) two pairs of double-layer multi-coil (MC) shimming structures suitable for local distortions and global inhomogeneity of the static magnetic field in the detection area of the microfluidic chip are proposed. (2) To reduce interference between the shimming multiple coils (MCs) and the microstrip RF coil, an RF field confinement structure and the corresponding RF tuning matching circuit are designed. (3) A double-layer MC shimming method based on local field distortions is proposed. The integrated probe incorporates two pairs of double-layer MC shimming plates and a pair of double-layer microstrip RF coils on both sides of the microfluidic chip, has a ground layer between the shim coils and the microstrip RF coils to shield interfering signals, and uses Bluetooth communication to transmit shimming data with the host. The proposed shimming method establishes an asymmetric distortion field model based on different microfluidic chip structures and samples, and then controls the inner and outer MCs to compensate for local distortions and global inhomogeneity of the magnetic field. Compared with traditional SH shimming, the proposed MC shimming method and system can flexibly achieve three-dimensional shimming of different target magnetic fields for local field distortion fields in planar microfluidic structures, and can use the small current in a single-turn coil to meet the shimming strength requirements. NMR experiments demonstrated that the proposed integrated probe and shimming method could significantly improve local magnetic field inhomogeneity caused by the magnetic susceptibility effects, enhance static magnetic field uniformity, and effectively improve the NMR signal resolution and spectral line shape. The integrated structural design provides a promising method for achieving high-performance on-chip detection and advancing device development for micro-sample NMR detection.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 10","pages":" 2419-2435"},"PeriodicalIF":6.1,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chiral organic–inorganic hybrid perovskites synthesized using an acoustofluidic closed system†","authors":"Tao Zhou, Yan Yu, Haonan Zhang, Chong Li, Ran Tao, Fujian Ren, Chen Fu, Jingting Luo and Yongqing Fu","doi":"10.1039/D4LC01073F","DOIUrl":"10.1039/D4LC01073F","url":null,"abstract":"<p >Chiral organic–inorganic hybrid perovskite films hold significant promise for optoelectronic applications due to their unique optical activity and excellent optoelectronic properties. However, their air and moisture sensitivity necessitate inert atmosphere processing, hindering practical application. In this work, we present a closed acoustofluidic system utilizing surface acoustic wave-based microcentrifugation for the synthesis of high-quality (<em>S</em>-MBA)<small>₂</small>PbI<small>₄</small> films. By confining both synthesis and film deposition within a sealed chamber, this approach eliminates air exposure, enabling the fabrication of films with enhanced crystallinity and a reduced band gap of 2.37 eV. The resulting chiral perovskite films exhibit significant circular dichroism, with an asymmetry factor of 9.3 × 10<small>⁻⁴</small>. Furthermore, control over film surface roughness (achieving &lt;0.6 μm) is demonstrated through modulation of acoustic operation parameters. The cost-effectiveness and versatility of this acoustic microcentrifugation system highlight its potential for advanced film material fabrication.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 9","pages":" 2278-2290"},"PeriodicalIF":6.1,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ monitoring of barrier function on-chip via automated, non-invasive luminescence sensing†","authors":"Bryan G. Schellberg, Abigail N. Koppes and Ryan A. Koppes","doi":"10.1039/D4LC01090F","DOIUrl":"10.1039/D4LC01090F","url":null,"abstract":"<p >Over the past 30 years, organs-on-a-chip (OOCs) have emerged as a robust alternative to address the technological challenges associated with current <em>in vitro</em> and <em>in vivo</em> options. Although OOCs offer improved bio-relevance and controlled complexity, broad adoption has remained limited. Most approaches to characterize on-chip structure and function require human intervention, limiting device translation and feasibility. Here, we introduce a new fiber optic-based sensing platform that enables automated, temporal luminescence sensing on-chip, validated for real-time readout of epithelial and endothelial barrier function under cytokine-induced inflammation. Our platform, capable of at least 1 μM resolution, tracked paracellular transport <em>in situ</em> for 9 days of culture under perfusion on-chip. These results offer an alternative sensing approach for continuous, non-invasive luminescence monitoring in OOCs.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 14","pages":" 3430-3443"},"PeriodicalIF":6.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-chip colorimetric assay for determining serum lithium concentration from whole blood†","authors":"Carl Olsson, Janosch Hauser, Federico Ribet, Fredrik Wikström, André Görgens, Olof Beck, Martin Schalling, Lena Backlund and Niclas Roxhed","doi":"10.1039/D5LC00044K","DOIUrl":"10.1039/D5LC00044K","url":null,"abstract":"<p >Lithium is the first-line treatment for bipolar disorder. However, the narrow therapeutic window of serum (s-)lithium is near its toxicity range, necessitating continuous monitoring of patients, a process involving regular hospital visits. On-demand home sampling could allow for more frequent testing, possibly resulting in safer patient outcomes, further dosage optimization, and increased compliance. This article presents a device that measures the s-lithium concentration from whole blood. The device consists of a single-use cartridge able to conduct on-chip serum filtration, volume-metering and an on-chip colorimetric assay. Spiked whole blood shows good linearity (Pearson's <em>r</em> = 0.96, <em>R</em><small>²</small> = 0.92), a limit-of-detection of 0.3 mmol L<small>⁻¹</small>, and an average deviation of 0.05 mmol L<small>⁻¹</small> (±6%) compared to atomic absorption spectroscopy. The on-chip colorimetric assay has shown to be a promising technique for measuring s-lithium concentration from whole blood and could allow patients to assess lithium levels at home and make the treatment available for new patient groups.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 9","pages":" 2270-2277"},"PeriodicalIF":6.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/lc/d5lc00044k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}