ACS Biomaterials Science & Engineering最新文献

{"title":"Multifunctional DNA-Collagen Biomaterials: Developmental Advances and Biomedical Applications.","authors":"Nikolaos Pipis, Bryan D James, Josephine B Allen","doi":"10.1021/acsbiomaterials.4c01475","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c01475","url":null,"abstract":"<p><p>The complexation of nucleic acids and collagen forms a platform biomaterial greater than the sum of its parts. This union of biomacromolecules merges the extracellular matrix functionality of collagen with the designable bioactivity of nucleic acids, enabling advances in regenerative medicine, tissue engineering, gene delivery, and targeted therapy. This review traces the historical foundations and critical applications of DNA-collagen complexes and highlights their capabilities, demonstrating them as biocompatible, bioactive, and tunable platform materials. These complexes form structures across length scales, including nanoparticles, microfibers, and hydrogels, a process controlled by the relative amount of each component and the type of nucleic acid and collagen. The broad distribution of different types of collagen within the body contributes to the extensive biological relevance of DNA-collagen complexes. Functional nucleic acids can form these complexes, such as siRNA, antisense oligonucleotides, DNA origami nanostructures, and, in particular, single-stranded DNA aptamers, often distinguished by their rapid self-assembly at room temperature and formation without external stimuli and modifications. The simple and seamless integration of nucleic acids within collagenous matrices enhances biomimicry and targeted bioactivity, and provides stability against enzymatic degradation, positioning DNA-collagen complexes as an advanced biomaterial system for many applications including angiogenesis, bone tissue regeneration, wound healing, and more.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic Air-Lifted Organ Culture System with a Protective Coverage Membrane for Full-Thickness Corneal Preservation.","authors":"Le Ma, Hongliang Jiang, Huan Wang, Ling Peng, Guoying Sun, Qiongyu Guo","doi":"10.1021/acsbiomaterials.4c02475","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02475","url":null,"abstract":"<p><p>Effective storage and utilization of limited donor corneal resources are in high demand to alleviate the shortage of donor corneal tissue. Here, we designed a static air-lifted organ culture system equipped with a protective coverage membrane, namely, an air-lifted OC-P system, to provide a biomimetic physiological environment for full-thickness corneal preservation. The air-lifted OC-P system features a unique collagen-based protective coverage membrane that can offer a moist, oxygen-rich environment for corneal epithelium, produce an appropriate intraocular pressure onto the cornea by gravity, and facilitate the maintenance of the organ culture medium level for nutrient supply during corneal preservation. Compared with conventional submerged and air-lifted corneal preservation methods, the air-lifted OC-P system remarkably improved the overall quality of the preserved corneas. These preserved corneas not only exhibited superior controllability of corneal swelling and extraordinary maintenance of the morphology and viability of all three types of corneal cells (i.e., corneal epithelium, keratocytes, and endothelium) but also demonstrated optimal optical, thermal, and mechanical properties. This air-lifted OC-P system presents a biomimetic strategy that provides a static and efficient method to replicate the corneal natural conditions for corneal preservation effectively.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-Delivery of Glycyrrhizin and Paclitaxel via Gelatin-Based Core-Shell Nanoparticles Ameliorates 1,2-Dimethylhydrazine-Induced Precancerous Lesions in Colon.","authors":"Md Meraj Ansari, Vivek Yadav, Sayali Dighe, Kaushik Kuche, Kanika, Rehan Khan, Sanyog Jain","doi":"10.1021/acsbiomaterials.4c02220","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02220","url":null,"abstract":"<p><p>Colorectal cancer is a lethal malignancy that begins from acquired/inherent premalignant lesions. Thus, targeting these lesions at an early stage of the disease could impede the oncogenesis and maximize the efficacy. The present work underscores a combinatorial therapy of paclitaxel (PTX) and glycyrrhizin (GL) delivered via gelatin-derived core-shell nanoparticles [AC-PCL(GL + PTX)-GNPs] for effective management of precancerous lesions. The desolvation method was adopted to prepare GL-loaded gelatin nanoparticles (GL-GNPs), which were coated with PTX and AC-PCL. The prepared NPs exhibited optimal physical attributes and had spherical morphology, as analyzed by transmission electron microscopy and field-emission scanning electron microscopy. <i>In vitro</i> release studies revealed sustained release for ∼96 h. Cell culture studies in HTC 116, and HT-29 cells showed synergistic action with CI < 0.9 and DRI > 1. Moreover, AC-PCL(GL + PTX)-GNPs exhibited amplified intracellular uptake and thus significantly reduced IC₅₀. Pharmacokinetic studies revealed substantiated pharmacokinetic parameters (AUC_0-∞, <i>C</i>_max, etc.). <i>In vivo</i> studies in a 1,2-dimethyl hydrazine-induced model revealed a decrease in the number of lesions, mucin depletion, and subside infiltrations. An immunohistochemical study revealed elevated expression of caspase-9 and suppressed expression of VEGF and <i>K</i>_i-67. Toxicity studies showed insignificant changes in systemic biomarkers along with no alterations in organ morphology and hemocompatibility. In essence, AC-PCL(GL + PTX)-GNPs render a competent and safer tactic to regulate early-stage precancerous lesions.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Octacalcium Phosphate Thin Film with Exposing Reactive Crystalline Plane in Biological Fluid.","authors":"Yanni Zhou, Zizhen Liu, Daichi Noda, Iori Yamada, Motohiro Tagaya","doi":"10.1021/acsbiomaterials.4c02011","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02011","url":null,"abstract":"<p><p>Octacalcium phosphate (OCP) has been used as a bone replacement material due to its higher bone affinity. However, the mechanism of affinity has not been clarified. Since the 100 crystalline plane of OCP is closely involved in the biological reactions during osteogenesis, it is important to expose the 100 crystalline plane of OCP to the biological fluid to precisely measure the interfacial reactions. In this study, the OCP plate-like crystals were fixed on a conductive substrate in the form of single-particle deposition, and the thin films with exposing 100 crystalline planes were fabricated. Then, the characteristics of hydration layers in the OCP crystals were enhanced by the exposure of 100 crystalline planes through the thin film formation, and the bioreactivity was found to be associated with the swelling and dissolution of the hydration layer in the biological fluid. Specifically, the OCP crystals were deposited on the gold sensor by electrophoretic deposition (<b>OCP/Au-1</b>). The results showed that the OCP plate-like crystals were selectively deposited on the gold sensor by electrophoresis. Subsequently, it was found that the ultrasonication of <b>OCP/Au-1</b> resulted in the formation of an OCP crystalline thin film (<b>m-OCP/Au-1</b>) with the single-particle thickness on the gold sensor with exposing 100 crystalline planes. Moreover, the FT-IR spectra of <b>m-OCP/Au-1</b> showed that the structure of the phosphate ions was rearranged by ultrasonication in the hydration layer, resulting in the regulation of the layered nanostructures, promoting higher crystallinity. Furthermore, the XPS spectra of <b>m-OCP/Au-1</b> indicated that the hydrogen phosphate ions in the hydration layer were exposed on the 100 crystalline plane of the topmost surface. The prepared <b>m-OCP/Au-1</b> was stable in citrate buffer, whereas it showed very high reactivity in phosphate buffer as the hydration layer gradually dissolved after the swelling, which was measured by the QCM-D technique. Therefore, the OCP crystalline thin films in this study were found to have higher surface reactivity due to the enhanced exposure of the hydration layer, which is assumed to be the cause of their bone-regeneration-promoting effect (i.e., higher bone affinity). The films in this study were stable at gastric acid pH and dissolved at neutral pH, which could make them useful as the orally administered drug carrier.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human 3D Lung Cancer Tissue Photothermal Therapy Using Zn- and Co-Doped Magnetite Nanoparticles.","authors":"Edynara Cruz de Moraes, Marcella Miranda Siqueira Furtuoso Rodrigues, Rafaela Campos de Menezes, Marcus Vinícius-Araújo, Marize Campos Valadares, Andris Figueiroa Bakuzis","doi":"10.1021/acsbiomaterials.4c01901","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c01901","url":null,"abstract":"<p><p>Iron oxide-based nanoparticles are promising materials for cancer thermal therapy and immunotherapy. However, several proofs of concept reported data with murine tumor models that might have limitations for clinical translation. Magnetite is nowadays the most popular nanomaterial, but doping with distinct ions can enhance thermal therapy, namely, magnetic nanoparticle hyperthermia (MNH) and photothermal therapy (PTT). In this study, we used a 3D alveolar reconstructed A549 lung cancer tissue model and investigated the thermal properties, toxicity, and impact of the thermal dose on tissue viability and inflammatory response using magnetite codoped with 40% Zn and 2% Co divalent ions. The ZnCo-doped magnetite nanoparticles are not toxic up to an NP concentration of 30 mg/mL. PTT showed a better heat generation response than MNH under the evaluated conditions, while NP showed a high external photothermal conversion efficiency of ∼1.3 g·L^-1·cm^-1 at 808 nm. PTT study is carried out at different temperatures, 43 and 47 °C, for 15 min. Tissue viability decreased with increasing thermal dose, while intracelullar ROS levels increased, mitochondrial activity decreased, and active caspase-3 increased, suggesting cell death via apoptosis. Nanoparticles and PTT did not influence the cytokine TNF, IL-10, IL-1B, and IL-12p70. In contrast, IL-6 and IL-8 were triggered by NP and PTT. Increased expression of IL-6 and IL-8 with higher thermal doses is correlated with tissue injury results, suggesting the potential role in activating and attracting immune cells to the site of thermal-mediated tissue injury.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage-Mediated Liquid Metal Nanoparticles for Enhanced Tumor Accumulation and Inhibition.","authors":"Yonggang Lv, Zhenghang Chen, Shuai Wang, Meizhen Zou","doi":"10.1021/acsbiomaterials.4c01130","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c01130","url":null,"abstract":"<p><p>In most studies, the penetration of nanoparticles into tumors was mainly dependent on the enhanced permeability and retention (ERP) effect. However, the penetration of nanoparticles would be limited by tumor-dense structure, immune system, and other factors. To solve these problems, macrophages with active tropism to tumor tissues, loaded nanoparticles with photothermal therapy, and chemotherapy were designed. In detail, liquid metal (gallium indium alloy) nanoparticles were modified with mesoporous silica and then embedded with the chemotherapeutic drug sorafenib (LM@Si/SO) for photothermal therapy and chemotherapy. After that, the LM@Si/SO nanoparticles were carried by the mouse macrophage RAW264.7 cell line (LM@Si/SO@R) to increase the accumulation of the nanoparticles in the tumor site and improve the tumor immune microenvironment. With the enhanced tumor accumulation, LM@Si/SO@R exhibited excellent antitumor ability in vitro and in vivo. Thus, these strategies via the cell carrier to enhance tumor therapeutic efficiency had the potential for the improvement of tumor therapy.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulating Arterial Stress for Rapid Evaluation of Antivascular Calcification Therapies from Herbal Extracts.","authors":"Yu Pan, Yuhang Zhang, Junsheng Lin, Zongtao Liu, Zhou Li, Zhi Luo, Nan Xu","doi":"10.1021/acsbiomaterials.4c01808","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c01808","url":null,"abstract":"<p><p>Vascular calcification severely disrupts cardiovascular hemodynamics, leading to high rates of morbidity and mortality. Despite their clinical impact, the development of effective treatments remains limited, underscoring an urgent need for efficient and reliable drug screening methods. Vascular smooth muscle cells (VSMCs) are known to play a central role in driving the calcification process, undergoing an osteogenic transition in response to pathological conditions. To mimic this process, we developed a cyclic stretching device that replicates the physiological mechanical stresses experienced by VSMCs during arterial pulsation. This device dramatically accelerates the osteogenic transition of VSMCs, reducing phenotypic switching from 13 days under static conditions to just 4 h. Using this device, we screened 20 herbal extracts for anticalcification properties and identified<i>Salvia miltiorrhiza</i>as a candidate with therapeutic potential that inhibits VSMC osteogenic transdifferentiation in vitro. The anticalcification efficacy of<i>Salvia miltiorrhiza</i>was further validated in a vitamin D-induced rat model of cardiovascular calcification, highlighting its translational potential. This screening platform provides a rapid and physiologically relevant method for evaluating potential antivascular calcification therapies, significantly improving the efficiency of drug discovery for clinical translation.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-Printed PCL Scaffolds Loaded with bFGF and BMSCs Enhance Tendon-Bone Healing in Rat Rotator Cuff Tears by Immunomodulation and Osteogenesis Promotion.","authors":"Yichao Ni, Bo Tian, Jinmin Lv, Dongxiao Li, Mingchao Zhang, Yuting Li, Yuanbin Jiang, Qirong Dong, Subin Lin, Jinzhong Zhao, Xingrui Huang","doi":"10.1021/acsbiomaterials.4c02340","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02340","url":null,"abstract":"<p><p>Rotator cuff tears are the most common conditions in sports medicine and attract increasing attention. Scar tissue healing at the tendon-bone interface results in a high rate of retears, making it a major challenge to enhance the healing of the rotator cuff tendon-bone interface. Biomaterials currently employed for tendon-bone healing in rotator cuff tears still exhibit limited efficacy. As a promising technology, 3D printing enables the customization of scaffold shapes and properties. Bone marrow mesenchymal stem cells (BMSCs) have multidifferentiation potential and valuable immunomodulatory effects. The basic fibroblast growth factor (bFGF), known for its role in proliferation, has been reported to promote osteogenesis. These properties make them applicable in tissue engineering. In this study, we developed a 3D-printed polycaprolactone (PCL) scaffold loaded with bFGF and BMSCs (PCLMF) to restore the tendon-bone interface and regulate the local inflammatory microenvironment. The PCLMF scaffolds significantly improved the biomechanical strength, histological score, and local bone mineral density at regenerated entheses at 2 weeks postsurgery and achieved optimal performance at 8 weeks. Furthermore, PCLMF scaffolds facilitated BMSC osteogenic differentiation and suppressed adipogenic differentiation both <i>in vivo</i> and <i>in vitro</i>. In addition, RNA-seq showed that PCLMF scaffolds could regulate macrophage polarization and inflammation through the MAPK pathway. The implanted scaffold demonstrated excellent biocompatibility and biosafety. Therefore, this study proposes a promising and practical strategy for enhancing tendon-bone healing in rotator cuff tears.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Anti-Inflammatory and Regenerative Potential: GelMA Hydrogel Loaded with IL-10 and Kartogenin for Intervertebral Disc Degeneration Therapy.","authors":"Shaofeng Yang, Jinhui Shi, Yusen Qiao, Yun Teng, Xianggu Zhong, Tianyi Wu, Chao Liu, Jun Ge, Huilin Yang, Jun Zou","doi":"10.1021/acsbiomaterials.4c01864","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c01864","url":null,"abstract":"<p><p>Intervertebral disc degeneration (IVDD) is a major contributor to chronic back pain and disability, with limited effective therapeutic options. Current treatment options, including conservative management and surgical interventions, often fail to effectively halt disease progression and come with notable side effects. IVDD is characterized by the breakdown of the extracellular matrix (ECM) and the infiltration of inflammatory cells, which exacerbate disc degeneration. This study presents a novel therapeutic strategy aimed at addressing the dual challenges of inflammation and ECM degradation in IVDD. We developed a gelatin methacryloyl (GelMA) hydrogel system loaded with interleukin-10 (IL-10), an anti-inflammatory cytokine, and kartogenin (KGN), a small-molecule compound known for its regenerative properties. The KGN + IL-10@GelMA hydrogel was designed to deliver these agents in a controlled manner directly to the degenerated disc, targeting both the inflammatory microenvironment and the promotion of nucleus pulposus (NP) tissue regeneration. In a puncture-induced IVDD model, this hydrogel system effectively delayed the degenerative progression and facilitated NP regeneration. Our findings suggest that the KGN + IL-10@GelMA hydrogel holds significant potential as a nonsurgical treatment option for IVDD, offering a promising approach to mitigate the progression of IVDD and enhance disc repair.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Hydroxyapatite-Aligned Collagen Sheets and Their Evaluation for Fibroblast Adhesion and Collagen Secretion.","authors":"Yuxuan Zhang, Gerardo Martin Quindoza, Hayato Laurence Mizuno, Yasuhiro Nakagawa, Toshiaki Tanaka, Yasutaka Anraku, Toshiyuki Ikoma","doi":"10.1021/acsbiomaterials.4c01617","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c01617","url":null,"abstract":"<p><p>The structure of many native tissues consists of aligned collagen (Col) fibrils, some of which are further composited with dispersed hydroxyapatite (HAp) nanocrystals. Accurately mimicking this inherent structure is a promising approach to enhance scaffold biocompatibility in tissue engineering. In this study, biomimetic sheets composed of highly aligned Col fibrils were fabricated using a plastic compression and tension method, followed by the deposition of HAp nanocrystals on the surface via an alternate soaking method. The fabricated Col sheets exhibited high solid density, retained the native periodicity (D-band) of Col fibrils, and displayed plate-like HAp nanocrystals dispersed on their surface. In vitro experiments demonstrated that these sheets could regulate fibroblasts adhesion, inducing more elongated nuclei and oriented actin bundles on the aligned Col sheets. Analysis of focal adhesion assembly revealed greater cell focal adhesions on the aligned composite sheets compared to those with random Col fibril structures. Fibroblasts cultured on aligned Col with partly HAp-mineralized sheets exhibited the highest cell-extracellular matrix (ECM) protein secretion, highlighting that HAp incorporation and fibroblast alignment synergistically promote early ECM formation and wound healing. These results suggest that highly aligned Col fibrils with dispersed HAp nanocrystals, closely mimicking the microarchitecture of natural tissues, have significant potential to control cell adhesion and protein secretion for tissue engineering applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}