ACS Biomaterials Science & Engineering最新文献

Enhancing Viability in Static and Perfused 3D Tissue Constructs Using Sacrificial Gelatin Microparticles.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-04-07 DOI: 10.1021/acsbiomaterials.4c02169

Andrew R Hudson, Daniel J Shiwarski, Alec J Kramer, Adam W Feinberg

{"title":"Enhancing Viability in Static and Perfused 3D Tissue Constructs Using Sacrificial Gelatin Microparticles.","authors":"Andrew R Hudson, Daniel J Shiwarski, Alec J Kramer, Adam W Feinberg","doi":"10.1021/acsbiomaterials.4c02169","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02169","url":null,"abstract":"Current limitations in engineered tissues arise from the inability to provide sufficient nutrients to cells deep within constructs, restricting their viability. This study focuses on enhancing diffusion by creating a microporous microenvironment using gelatin microparticles within collagen scaffolds. By leveraging the FRESH (Freeform Reversible Embedding of Suspended Hydrogels) 3D bioprinting technique, gelatin microparticles are utilized both as a support material and as a thermoresponsive porogen to establish interconnected pores. The results indicate that scaffolds with 75% porosity significantly increase diffusion rates and cell viability, extending beyond the conventional ∼200 μm limit. Additionally, integrating vascular-like channels with porous scaffolds and applying perfusion improved nutrient transport, leading to enhanced cell survival in larger constructs. This combination of microporosity and perfusion represents a promising approach to create thicker tissues without necrotic regions, potentially paving the way for scalable tissue engineering applications. The findings suggest that optimizing pore sizes and scaffold perfusion can bridge the gap between rapid tissue formation and slower vascularization processes, enabling the future development of functional tissue constructs at clinically relevant scales.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano-Enabled Effective Tuberculosis Treatments: A Concise Overview.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-04-07 DOI: 10.1021/acsbiomaterials.4c02109

Jingjing Li, Huxiao Sun, Dzmitry Shcharbin, Serge Mignani, Jean-Pierre Majoral, Mingwu Shen, Xiangyang Shi

引用次数: 0

Esophageal Reconstruction with Myogenesis-Inducing Gene Transfected Mesenchymal Stem Cell-Seeded Film with Leaf-Stacked Structure.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-04-04 DOI: 10.1021/acsbiomaterials.4c02396

Hanaro Park, Hye-Joung Kim, In Gul Kim, Min Ji Kim, Yewon Kim, So Young Eom, Jungirl Seok, Se Heang Oh, Eun-Jae Chung

{"title":"Esophageal Reconstruction with Myogenesis-Inducing Gene Transfected Mesenchymal Stem Cell-Seeded Film with Leaf-Stacked Structure.","authors":"Hanaro Park, Hye-Joung Kim, In Gul Kim, Min Ji Kim, Yewon Kim, So Young Eom, Jungirl Seok, Se Heang Oh, Eun-Jae Chung","doi":"10.1021/acsbiomaterials.4c02396","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02396","url":null,"abstract":"In the domain of tissue engineering and regenerative medicine, artificial replacements have been developed as viable options for esophageal reconstruction and serve as alternatives to traditional surgical procedures. Restoration of smooth muscle functionality is crucial in esophageal regeneration. We evaluated the efficacy of esophageal reconstructions in an animal model, using tissue-engineered films with a leaf-stacked structure (FLSS), seeded with mesenchymal stem cells (MSCs), which were genetically modified with myogenic genes. Esophageal partial defects were variously reconstructed in animals (n = 8 per group, except the no-implantation group), categorized as (1) normal rats; (2) rats implanted with naked FLSS; (3) rats implanted with FLSS with MSCs; (4) rats implanted using FLSS with myogenesis-inducing gene transfected MSCs; and (5) rats without implantation at the defect site (n = 3). The FLSS exhibited appropriate mechanical characteristics for transplantation. Successful repair of esophageal defects was observed with significantly enhanced epithelial regeneration in the MSC-seeded FLSS group compared to that in the naked FLSS group. Moreover, smooth muscle regeneration was notably higher in the FLSS with myogenesis-inducing gene transfected MSCs than in the group without myogenic gene transfection. The myogenesis-inducing gene-transfected MSC-seeded FLSS group showed a tendency toward increased smooth muscle regeneration, this indicates that FLSS with myogenesis-inducing genes transfected MSC may contribute positively to the maintenance of function in the reconstructed esophagus.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Esophageal Reconstruction with Myogenesis-Inducing Gene Transfected Mesenchymal Stem Cell-Seeded Film with Leaf-Stacked Structure

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-04-04 DOI: 10.1021/acsbiomaterials.4c0239610.1021/acsbiomaterials.4c02396

Hanaro Park, Hye-Joung Kim, In Gul Kim, Min Ji Kim, Yewon Kim, So Young Eom, Jungirl Seok, Se Heang Oh* and Eun-Jae Chung*,

{"title":"Esophageal Reconstruction with Myogenesis-Inducing Gene Transfected Mesenchymal Stem Cell-Seeded Film with Leaf-Stacked Structure","authors":"Hanaro Park, Hye-Joung Kim, In Gul Kim, Min Ji Kim, Yewon Kim, So Young Eom, Jungirl Seok, Se Heang Oh* and Eun-Jae Chung*, ","doi":"10.1021/acsbiomaterials.4c0239610.1021/acsbiomaterials.4c02396","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02396https://doi.org/10.1021/acsbiomaterials.4c02396","url":null,"abstract":"In the domain of tissue engineering and regenerative medicine, artificial replacements have been developed as viable options for esophageal reconstruction and serve as alternatives to traditional surgical procedures. Restoration of smooth muscle functionality is crucial in esophageal regeneration. We evaluated the efficacy of esophageal reconstructions in an animal model, using tissue-engineered films with a leaf-stacked structure (FLSS), seeded with mesenchymal stem cells (MSCs), which were genetically modified with myogenic genes. Esophageal partial defects were variously reconstructed in animals (n = 8 per group, except the no-implantation group), categorized as (1) normal rats; (2) rats implanted with naked FLSS; (3) rats implanted with FLSS with MSCs; (4) rats implanted using FLSS with myogenesis-inducing gene transfected MSCs; and (5) rats without implantation at the defect site (n = 3). The FLSS exhibited appropriate mechanical characteristics for transplantation. Successful repair of esophageal defects was observed with significantly enhanced epithelial regeneration in the MSC-seeded FLSS group compared to that in the naked FLSS group. Moreover, smooth muscle regeneration was notably higher in the FLSS with myogenesis-inducing gene transfected MSCs than in the group without myogenic gene transfection. The myogenesis-inducing gene-transfected MSC-seeded FLSS group showed a tendency toward increased smooth muscle regeneration, this indicates that FLSS with myogenesis-inducing genes transfected MSC may contribute positively to the maintenance of function in the reconstructed esophagus.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 4","pages":"2274–2289 2274–2289"},"PeriodicalIF":5.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances in Peptide-Functionalized Hydrogels for Bone Tissue Engineering.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-04-03 DOI: 10.1021/acsbiomaterials.4c02198

Guanrong Li, Yang Luo, Zeming Hu, Zheyuan Shi, Xu Cao, Rong Xu, Yunfeng Mi, Yudong Yao, Haijiao Mao, Hua Zhang, Yingchun Zhu

{"title":"Recent Advances in Peptide-Functionalized Hydrogels for Bone Tissue Engineering.","authors":"Guanrong Li, Yang Luo, Zeming Hu, Zheyuan Shi, Xu Cao, Rong Xu, Yunfeng Mi, Yudong Yao, Haijiao Mao, Hua Zhang, Yingchun Zhu","doi":"10.1021/acsbiomaterials.4c02198","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02198","url":null,"abstract":"Efficient therapeutic approaches for bone regeneration are urgently required to address the significant challenges associated with the repair of large-scale or long-segment bone defects. Peptide-functionalized hydrogels (PFHs) have emerged as important bioactive materials in bone tissue engineering because they produce biomimetic microenvironments enriched with multiple biochemical signals. This review summarizes the key fabrication techniques for PFHs and discusses their diverse applications in different fields. Furthermore, we systematically highlighted the biochemical functionalization of PFHs, which includes basic functions such as cell adhesion, cell recruitment, and osteoinduction; improved functions such as angiogenesis, biomineralization, immune regulation, and hormone regulation; and other functions, including antimicrobial and antitumor effects. Finally, critical biosafety considerations associated with PFHs and perspectives on developing intelligent PFHs are addressed. This review aims to inspire further research on PFHs and accelerate their applications in bone tissue engineering.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clearance of Cell-Free DNA: A Novel Target for Therapeutic Utilization in Multiple Systemic Disorders

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-04-03 DOI: 10.1021/acsbiomaterials.5c0004910.1021/acsbiomaterials.5c00049

Wenjun Zhang, Xuan Jing, Bing Li* and Xiuping Wu*,

引用次数: 0

Clearance of Cell-Free DNA: A Novel Target for Therapeutic Utilization in Multiple Systemic Disorders.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-04-03 DOI: 10.1021/acsbiomaterials.5c00049

Wenjun Zhang, Xuan Jing, Bing Li, Xiuping Wu

引用次数: 0

Retraction of “Fabrication of Oro-Dispersible Sodium Valproate-Loaded Nanofibrous Patches for Immediate Epileptic Innervation”

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-04-03 DOI: 10.1021/acsbiomaterials.5c0043210.1021/acsbiomaterials.5c00432

Ece Guler, Humeyra B. Yekeler, Zarife N. Ozdemir Kumral, Gita Parviz, Gul S. Ozcan, Burcu Uner, Sinem G. Demirbas, Simge Ayyildiz, Yusufhan Yazir, Deepak Kalaskar* and Muhammet E. Cam*,

引用次数: 0

Recent Advances in Peptide-Functionalized Hydrogels for Bone Tissue Engineering

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-04-03 DOI: 10.1021/acsbiomaterials.4c0219810.1021/acsbiomaterials.4c02198

Guanrong Li, Yang Luo, Zeming Hu, Zheyuan Shi, Xu Cao, Rong Xu, Yunfeng Mi, Yudong Yao, Haijiao Mao*, Hua Zhang* and Yingchun Zhu*,

{"title":"Recent Advances in Peptide-Functionalized Hydrogels for Bone Tissue Engineering","authors":"Guanrong Li, Yang Luo, Zeming Hu, Zheyuan Shi, Xu Cao, Rong Xu, Yunfeng Mi, Yudong Yao, Haijiao Mao*, Hua Zhang* and Yingchun Zhu*, ","doi":"10.1021/acsbiomaterials.4c0219810.1021/acsbiomaterials.4c02198","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02198https://doi.org/10.1021/acsbiomaterials.4c02198","url":null,"abstract":"Efficient therapeutic approaches for bone regeneration are urgently required to address the significant challenges associated with the repair of large-scale or long-segment bone defects. Peptide-functionalized hydrogels (PFHs) have emerged as important bioactive materials in bone tissue engineering because they produce biomimetic microenvironments enriched with multiple biochemical signals. This review summarizes the key fabrication techniques for PFHs and discusses their diverse applications in different fields. Furthermore, we systematically highlighted the biochemical functionalization of PFHs, which includes basic functions such as cell adhesion, cell recruitment, and osteoinduction; improved functions such as angiogenesis, biomineralization, immune regulation, and hormone regulation; and other functions, including antimicrobial and antitumor effects. Finally, critical biosafety considerations associated with PFHs and perspectives on developing intelligent PFHs are addressed. This review aims to inspire further research on PFHs and accelerate their applications in bone tissue engineering.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 4","pages":"1970–1989 1970–1989"},"PeriodicalIF":5.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction of "Fabrication of Oro-Dispersible Sodium Valproate-Loaded Nanofibrous Patches for Immediate Epileptic Innervation".

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-04-03 DOI: 10.1021/acsbiomaterials.5c00432

Ece Guler, Humeyra B Yekeler, Zarife N Ozdemir Kumral, Gita Parviz, Gul S Ozcan, Burcu Uner, Sinem G Demirbas, Simge Ayyildiz, Yusufhan Yazir, Deepak Kalaskar, Muhammet E Cam

引用次数: 0