ACS Biomaterials Science & Engineering最新文献

{"title":"Porous and Flexible Polyetheretherketone (PEEK) Mesh Based on Filament Fused Fabrication Technology: A Balance between Mechanical and Biological Characteristics for Guided Bone Regeneration.","authors":"Ti Yu, Qiang Wei, Junyi Zhao, Qianrong Xiang, Haiyang Yu","doi":"10.1021/acsbiomaterials.5c00309","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00309","url":null,"abstract":"<p><p>Space maintenance and stability of the barrier membrane play a more crucial role than cell occlusion in alveolar bone augmentation. Polyetheretherketone (PEEK) is a promising alternative to titanium mesh due to its remarkable biocompatibility and mechanical properties that match natural bone. This study designed and manufactured a highly porous, flexible, and palisade-like PEEK membrane with pore diameters of 300 μm, 500 μm, and 700 μm through fused filament fabrication (FFF) technology. The optimum pore size fulfilling the equilibrium between mechanical properties and biological behavior was explored for severe alveolar bone regeneration. Printing accuracy, surface structural characteristics, roughness, hydrophilicity, and mechanical properties of the PEEK membranes were evaluated. Finite element analysis (FEA) was conducted to analyze the stress and strain distribution in the guided bone regeneration (GBR) model. Cell morphology, viability, and osteogenic differentiation were carried out utilizing human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hWJ-MSCs) in vitro. The calvarial defect of rabbits in critical size was established, and new bone formation at 4 and 8 weeks after implantation was evaluated by micro-CT and histomorphometry. The results showed that the PEEK meshes, accompanied by one rough surface and another smooth side, exhibited great printing accuracy and hydrophobicity. The mechanical properties were inversely proportional to the pore diameter of the PEEK mesh, yet all groups had satisfactory stretchability. FEA indicated great stress dispersion, spatial retention, and mucosal integrity preservation in PEEK-300 and PEEK-500. In vitro tests showed that the macropores of PEEK-500 and PEEK-700 promoted greater hWJ-MSC migration and osteogenic differentiation. PEEK-500 demonstrated relatively higher new bone formation and stronger trabeculae at 8 weeks after implantation in vivo. Based on the palisade-like and flexible configuration, our findings supported that the 3D-printed PEEK mesh with appropriate pore size was capable of achieving a balance between mechanical and biological characteristics, showing potential for application in GBR.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovations in Core-Shell Electrospinning: A Comprehensive Review in Recent Advances of Core-Shell Electrospun Polylactic Acid Nanocomposite Fibers for Potential Biomedical Applications.","authors":"B Akhila, V Abhijith, Mridula Sreedharan, Lakshmipriya Ravindran, Aiswarya Sathian, Sabu Thomas, Sreekala Meyyarappallil Sadasivan","doi":"10.1021/acsbiomaterials.5c00194","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00194","url":null,"abstract":"<p><p>The unique structural and functional properties of polylactic acid (PLA) nanofibers, particularly in core-shell structures, have placed them as a crucial material in biomedical engineering. In addition to its renewable characteristics, biodegradability, and biocompatibility, PLA distinguishes itself and satisfies the increasing demand for environmentally friendly and sustainable materials in medical applications. It is an optimal material for scaffolds, implants, and biomedical devices due to its adjustable mechanical strength, degradation rate, excellent biocompatibility, and capacity to form intricate fiber architectures. The precise manipulation of PLA nanofibers can be made easier by advanced electrospinning techniques, which maintain the structural integrity of the PLA nanofibers while allowing for the encapsulation with controlled release of bioactive compounds. The core-shell architectures enhance mechanical performance, cellular adhesion, and proliferation, making them suitable for various advanced biomedical applications. Moreover, PLA degradation products have a much lower environmental effect compared to other synthetic nondegradable polymers, signifying a substantial advantage. The review article covers the techniques used for the fabrication of coaxial electrospun PLA nanofibers, their benefits, and potential uses in innovative healthcare products and sustainable biomedical practices.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mentors as Allies for Disabled Scientists.","authors":"Emilio I Alarcón","doi":"10.1021/acsbiomaterials.5c00406","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00406","url":null,"abstract":"<p><p>Close to 28% of the population in the United States and Canada is living with disability, yet despite this prevalence, persons with disabilities remain mostly absent in biomedical research. While the role of mentors has been demonstrated to be effective in increasing the number of women and other groups in biomedical research, mentoring disabled scientists remains a gray area. In this short perspective, I share my personal experience as a person with a disability who was mentored by a nondisabled person, who ultimately became an ally.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Vertical Transmission of Exosomes in Diagnostic and Therapeutic Targets for Pregnancy Complications.","authors":"Shrikrishna Bhagat, Rakshith Hanumanthappa, Ketki Bhokare, Neelabh Datta, Nidhi Vastrad, M David, N Maharaj, Krishnan Anand","doi":"10.1021/acsbiomaterials.5c00119","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00119","url":null,"abstract":"<p><p>During pregnancy, the mother-placenta relationship involves intricate and dynamic exchanges. Over the course of gestation, the maternal body encounters numerous fetal materials secreted by the placenta, such as hormones, growth factors, and extracellular vesicles like exosomes. These exosomes are carriers of key biomolecules, including proteins, lipids, nucleic acids (DNA), and microRNA (miRNA), capable of influencing maternal cellular activity. Although the exact functions of placental exosomes during pregnancy remain under investigation, existing research indicates that they contribute significantly to normal placental growth and maternal immune tolerance, both of which are vital for sustaining a healthy pregnancy. The involvement of exosomes in the etiology and progression of pregnancy complications is also under investigation. Variations in the quality and quantity of placenta-derived exosomes, their concentration in maternal plasma, and their composition and bioactivity have been linked to complications such as gestational diabetes, preeclampsia, and maternal infections. There is considerable interest not only in understanding the role of placenta-derived exosomes in both normal and complicated pregnancies but also in their potential as biomarkers and therapeutic targets. Progress in this field depends on using specific and well-characterized methodologies and techniques to precisely determine the role of exosomes in pregnancy complications and their clinical utility. This review emphasizes the significance of placenta-derived exosomes in pregnancy, focusing on their interaction with the maternal system. Additionally, it explores new techniques and ideas for analyzing placental exosomes as potential biomarkers for the early diagnosis of pregnancy complications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical Stimulation of Equine Bone Marrow Mesenchymal Stromal Cell-Derived Cartilage-Like In Vitro Model Triggers Osteoarthritis Features.","authors":"Romain Contentin, Cassie Jehl, Kevin Commenchail, Florence Legendre, Philippe Galéra, Frédéric Cassé, Magali Demoor","doi":"10.1021/acsbiomaterials.5c00500","DOIUrl":"10.1021/acsbiomaterials.5c00500","url":null,"abstract":"<p><p>Osteoarthritis (OA) affects millions of people globally, causing irreversible cartilage damage, chronic inflammation, and progressive joint dysfunction. Similarly, horses can develop OA spontaneously or due to their athletic careers, influenced by mechanical and biochemical factors. Current treatments primarily focus on symptom relief without promoting cartilage regeneration. In line with the 3Rs principles (refine, reduce, replace), the development of <i>in vitro</i> OA models is essential for advancing new therapeutic approaches against OA. In response to this need, the present study aimed to develop an <i>in vitro</i> model of mechanically induced OA. Bone marrow-derived mesenchymal stromal cells (BM-MSCs) were cultured in a biomaterial scaffold and differentiated for 21 days using a chondrogenic medium to produce cartilage-like <i>in vitro</i> models. The cartilage-like <i>in vitro</i> models underwent mechanical stimulation (compression) for 3 and 7 days at pressures sufficient to induce injurious stress. BM-MSC-derived chondrocytes express the transient receptor potential vanilloid-type 4 (TRPV4) channel and are responsive to mechanical stimulation. Mechanical stimulation was found to reduce cell proliferation without inducing cell death. The overall protein levels of type II collagen drastically declined after both 3 and 7 days of mechanical stimulation. Additionally, glycosaminoglycan (GAG) content within the cartilage-like <i>in vitro</i> models decreased, whereas GAG release into the supernatant increased following mechanical stimulation. Ultimately, compression led to the upregulation of catabolic factors and inflammatory mediators. In conclusion, this model successfully replicates several key features of OA, making it a valuable tool for investigating the disease's mechanisms and testing new therapeutic strategies.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant Small Leucine-Rich Proteoglycans Modulate Fiber Structure and Mechanical Properties of Collagen Gels.","authors":"Serafina G Lopez, Henrique Reis Moura, Erik Chow, Joe Chin-Hun Kuo, Matthew J Paszek, Lawrence J Bonassar","doi":"10.1021/acsbiomaterials.5c00732","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00732","url":null,"abstract":"<p><p>Collagen is a key extracellular matrix protein found in connective tissues. The structure and organization of collagen fibers play a crucial role in determining tissue function and how tissues respond to mechanical loads. Small leucine-rich proteoglycans (SLRPs) are well-known facilitators of collagen fibrillogenesis in connective tissues. While the role of SLRPs has been extensively documented in tissues such as tendon and skin, their functions are primarily inferred from changes observed in knockout models. Additionally, their specific roles and influences of their addition to a system, particularly in collagen gel-based materials, remain underexplored. Previous in vitro studies of SLRPs have been partly limited by the challenges associated with obtaining pure SLRPs in sufficient quantities and with appropriate glycosylation. Therefore, novel methods to reliably produce SLRPs at the required quality and scale are needed. In this study, we first evaluated the feasibility of producing recombinant decorin, biglycan, and fibromodulin using HEK293-F cells. Subsequently, we investigated the effect of SLRP supplementation on high-density collagen gels using scanning electron microscopy and assessed the impact on tensile properties. Our findings demonstrated that each SLRP uniquely influenced collagen structure at both the fibril and fiber levels, consequently modifying the tissues' mechanical response to load. Decorin, in particular, exhibited significant differences in tensile properties compared to biglycan and fibromodulin, underscoring its distinct role in promoting a structurally and mechanically robust response under tensile load.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Damage Associated Molecular Patterns (DAMPs) Mediate the Foreign Body Response to Poly(ethylene glycol) Diacrylate Hydrogels via Toll like Receptors.","authors":"Brittany J Thompson, Leila S Saleh, Emma L Carillion, Scott Alper, Stephanie J Bryant","doi":"10.1021/acsbiomaterials.4c01984","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c01984","url":null,"abstract":"<p><p>Poly(ethylene glycol) hydrogels prepared from PEG diacrylate (PEGDA) monomers are widely investigated for biological applications including drug delivery and tissue engineering. Despite their high-water contents, PEGDA hydrogels when implanted into the body induce a foreign body response (FBR). The overall goal of this study was to investigate the role of surface adsorbed proteins in the FBR to PEGDA hydrogels and determine whether they act as damage associated molecular patterns (DAMPs) to initiate inflammation. Toll-like receptors (TLRs) 2 and 4 are one of the primary receptors that recognize DAMPs. In vitro and in vivo studies were performed using wildtype (Wt), TLR2^-/-, TLR4^-/-, and TLR2^-/-TLR4^-/- double knockout (DKO) mice. In vitro, Wt neutrophils were activated in response to the PEGDA hydrogels as measured by myeloperoxidase, and this response was partially mediated by TLR4 but not TLR2. Wt macrophages predisposed to an inflammatory state responded to the PEGDA hydrogel itself and to a greater extent to surface-adsorbed plasma by producing the pro-inflammatory cytokines interleukin 6 and tumor necrosis factor α. TLR4 and to a lesser extent TLR2 mediated this response. To link DAMPs to the FBR in vivo, PEGDA hydrogels were implanted subcutaneously in mice. The thickness of the inflammatory cell layer was mediated by both TLR2 and TLR4 as knocking out both TLRs led to significantly fewer inflammatory cells. The fibrous capsule was reduced by 50% in both single KO mice as well as the DKO mice. Taken together, this study determined that DAMPs formed from surface-adsorbed plasma activate TLR4. In the more complex in vivo environment, both TLR2 and TLR4 are major contributors to the inflammatory response and partial contributors to the fibrous encapsulation. Overall, these findings provide a critical link between DAMPs, TLRs, immune cells, and the FBR to PEGDA hydrogels.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary Study on Carboxymethyl Chitosan-Based Carbon Dots for Tracing and Promoting Osteogenic Differentiation.","authors":"Xiao Ning, Mingrui Zong, Jiahui Tong, Huaiyi Cheng, Yuxin Cao, Jinrong Liu, Jianing Ren, Jiadi Li, Ran Zhang, Xiuping Wu, Bing Li","doi":"10.1021/acsbiomaterials.5c00135","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00135","url":null,"abstract":"<p><p>Maxillofacial bone defects, a common challenge in oral and maxillofacial surgery, affect over 2 million patients globally each year due to tumor resection, trauma, or infection. Beyond their role in mastication and speech, the structural integrity of jaw bones is critical for facial aesthetics. Current clinical treatments rely on autologous bone grafts, which are limited by donor site morbidity, or allografts with immune rejection risks. Synthetic materials (e.g., titanium alloys, hydroxyapatite) offer mechanical stability but lack bioactivity for efficient osseointegration. Natural polysaccharide-based materials like chitosan have gained attention for their biocompatibility and cell adhesion properties, yet their derivative, carboxymethyl chitosan (CMC), faces limitations such as poor mineralization induction and uncontrollable degradation rates. This study aimed to develop carboxymethyl chitosan-based carbon dots (C-CDs) via citric acid (CA) modification, endowing the material with dual functionalities: temporal regulation of osteogenesis and mineralization through surface polar groups, and cellular tracing via graphitized carbon core fluorescence. The results showed that C-CDs exhibited excellent fluorescence properties and biocompatibility, enhanced ALP activity, and upregulated osteogenic genes (Alp, Runx2, Sp-7, OCN) to promote osteogenic differentiation. In animal studies, the C-CDs group had a significantly higher bone volume fraction (BV/TV) than controls, with histological analysis revealing typical lamellar bone structures, indicating effective promotion of bone regeneration. This innovation addresses the shortcomings of conventional materials for Maxillofacial bone defect repair.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical Comparison of <i>Escherichia coli</i> Biofilms with Altered Matrix Composition: A Study Combining Shear-Rheology and Microindentation.","authors":"Macarena Siri, Adrien Sarlet, Ricardo Ziege, Laura Zorzetto, Carolina Sotelo Guzman, Shahrouz Amini, Regine Hengge, Kerstin G Blank, Cécile M Bidan","doi":"10.1021/acsbiomaterials.5c00261","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00261","url":null,"abstract":"<p><p>The mechanical properties of bacterial biofilms depend on the composition and microstructure of their extracellular matrix (ECM), which constitutes a network of extracellular proteins and polysaccharide fibers. In particular, <i>Escherichia coli</i> macrocolony biofilms were suggested to present tissue-like elasticity due to a dense fiber network consisting of amyloid curli and phosphoethanolamine-modified cellulose (pEtN-cellulose). To understand the contribution of these two main ECM components to the emergent mechanical properties of <i>E. coli</i> biofilms, we performed shear-rheology and microindentation experiments on biofilms grown from <i>E. coli</i> strains that produce different ECM. We measured that biofilms containing curli fibers are stiffer in compression than curli-deficient biofilms. We further quantitatively demonstrate the crucial contribution of pEtN-cellulose, and especially of the pEtN modification, to the stiffness and structural stability of biofilms when associated with curli fibers. To compare the differences observed between the two methods, we also investigated how the structure and mechanical properties of biofilms with different ECM compositions are affected by the sample preparation method used for shear-rheology. We found that biofilm homogenization, used prior to shear-rheology, destroys the macroscale structure of the biofilm while the microscopic ECM architecture may remain intact. The resulting changes in biofilm mechanical properties highlight the respective advantages and limitations of the two complementary mechanical characterization techniques in the context of biofilm research. As such, our work does not only describe the role of the ECM on the mechanical properties of <i>E. coli</i> biofilms. It also informs the biofilm community on considering sample preparation when interpreting mechanical data of biofilm-based materials.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intranasal Delivery of Ivermectin Nanosystems as an Antitumor Agent: Focusing on Glioma Suppression.","authors":"Maiara Callegaro Velho, Valeria Luiza Winck, Camila da Silveira Mariot, Juliete Nathali Scholl, Augusto Ferreira Weber, Rita de Kássia Souza, Fernanda Visioli, Fabrício Figueiró, Monique Deon, Diogo André Pilger, Ruy Carlos Ruver Beck","doi":"10.1021/acsbiomaterials.5c00642","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00642","url":null,"abstract":"<p><p>Glioblastoma presents significant challenges in neuro-oncology due to its aggressive nature, drug resistance, and restrictions imposed by the blood-brain barrier. Ivermectin (IVM), known for its antiparasitic properties, has been highlighted as a promising treatment for tumors and an alternative therapy for glioma, although it exhibits low oral bioavailability. Therefore, we investigated the <i>in vivo</i> effect of IVM encapsulation in organic and inorganic nanosystems, first screened <i>in vitro</i> against different tumor cells and subsequently evaluated <i>in vitro</i> and <i>in vivo</i> glioma models. We produced IVM-loaded poly(ε-caprolactone) nanocapsules (IVM-NC) using the interfacial deposition method, and IVM-loaded nanostructured silica particles (IVM-MCM) by loading IVM into commercial MCM-41 silica using the incipient wetness method. IVM-NC had a nanometric size (190 nm), a unimodal size distribution (span <2), and a high encapsulation efficiency (100% at 1 mg/mL). IVM-MCM exhibited a well-organized hexagonal mesoporous structure and high drug loading (0.12 mg/mg). Nanoencapsulated IVM significantly reduced the viability of various cancer cell lines, particularly glioma cell lines, which led us to evaluate them in a preclinical glioma model. We implanted adult male Wistar rats with C6 cells. Intranasal delivery of IVM-NC (60 μg/rat/day for 10 days) resulted in a larger decrease in tumor size compared with the group treated with free IVM, along with histopathological improvements. Treatment with IVM-MCM did not decrease the tumor size. However, both treatments were well-tolerated, with no adverse effects on weight, biochemical, or hematological parameters, or lung histology. Furthermore, the effective equivalent dose of IVM (26 μg/kg) in the rat glioma model was lower than the approved human dose for parasitic infections. This study marks the first exploration of IVM delivery to the brain. In summary, nasal administration of nanoencapsulated IVM via nanocapsules presents a promising avenue for targeted therapy against glioblastoma, with potential implications for clinical translation.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}