Multifunctional Metal Oxide-Doped Nanofluorophosphate Glass: A Bioactive Topical Formulation for Ischemic Wound Repair.

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2025-09-25 DOI:10.1021/acsbiomaterials.5c01239

Mareeswari Balasubramanian, Sundara Moorthi Ganesan, Pugalanthipandian Sankaralingam, Vijayakumar Chinnaswamy Thangavel, Ravichandran Kandaswamy

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引用次数: 0

Abstract

Hypovascular or ischemic ulcer healing remains a significant challenge in regenerative medicine. Here, we report a novel topical formulation incorporating metal oxide-doped fluorophosphate (FP) glass to accelerate the healing of ischemic ulcers through enhanced angiogenesis and fibroblast migration. The bioactive FP glass nanoparticles (ZnFP, MgFP, and AgFP) were integrated with polymeric bases (PPF, 1,2-Diol, PEG, and PPG) to form biocompatible, nontoxic topicals. The formulations were systematically evaluated in vitro for cytotoxicity, migration assays, and angiogenesis potential using Chorioallantoic Membrane (CAM) assays and in vivo on full thickness cut and burn wound models. The optimized MgFP-PPG formulation exhibited a 9.8-fold increase in Epithelial Growth Factor (EGF) expression compared to that of controls, while AgFP-PPG enhanced Vascular Endothelial Growth Factor (VEGF) secretion by 4.2-fold. Scratch assays demonstrated considerably faster fibroblast migration, and CAM assays confirmed enhanced neovascularization with MgFP. In vivo, the MgFP-PPG formulation resulted in 72.5% wound contraction by day 7, compared to 61.3% with silver sulfadiazine and 45.8% in untreated wounds. Histopathological evaluation further revealed greater granulation tissue formation, increased Cluster of Differentiation 34 (CD34) expression, and enhanced VEGF signaling in burn wound models treated with MgFP-PPG and appreciably enhanced the wound healing by promoting cellular proliferation (Ki67). This study presents a promising approach for next-generation ischemic wound healing therapies.

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多功能金属氧化物掺杂纳米氟磷酸盐玻璃：用于缺血性伤口修复的生物活性局部配方。

低血管或缺血性溃疡愈合仍然是再生医学的重大挑战。在这里，我们报告了一种新的局部配方，其中包含金属氧化物掺杂的氟磷酸盐（FP）玻璃，通过增强血管生成和成纤维细胞迁移来加速缺血性溃疡的愈合。生物活性FP玻璃纳米颗粒（ZnFP， MgFP和AgFP）与聚合物碱（PPF, 1,2-二醇，PEG和PPG）结合形成生物相容性，无毒的外用物。采用绒毛尿囊膜（CAM）实验系统地评估了这些配方的体外细胞毒性、迁移试验和血管生成潜力，并在体内对全层切口和烧伤模型进行了评估。优化后的MgFP-PPG制剂与对照组相比，上皮生长因子（EGF）表达量增加9.8倍，而血管内皮生长因子（VEGF）分泌量增加4.2倍。划痕实验显示成纤维细胞迁移速度明显加快，CAM实验证实MgFP增强了新生血管的形成。在体内，MgFP-PPG制剂在第7天导致72.5%的伤口收缩，而磺胺嘧啶银的伤口收缩率为61.3%，未治疗的伤口收缩率为45.8%。组织病理学评估进一步显示，MgFP-PPG处理的烧伤创面模型肉芽组织形成增加，CD34表达增加，VEGF信号传导增强，并通过促进细胞增殖明显促进创面愈合（Ki67）。该研究为下一代缺血性伤口愈合治疗提供了一种有希望的方法。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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