IF 9.4 1区化学

Journal of Colloid and Interface Science Pub Date : 2025-05-15 Epub Date: 2025-02-07 DOI: 10.1016/j.jcis.2025.01.183

Fangjie Xi, Leilei Zhang, Anying Cheng, Hua Sun, Yibo Qin, Baocheng Yang, Shouren Zhang, Junying Ma, Xiaoqiang Du, Xiangyu Meng

{"title":"Molten salt synthesis of 1T phase dominated O-MoS2 for enhancing photocatalytic hydrogen production performance of CdS via Ohmic junction.","authors":"Fangjie Xi, Leilei Zhang, Anying Cheng, Hua Sun, Yibo Qin, Baocheng Yang, Shouren Zhang, Junying Ma, Xiaoqiang Du, Xiangyu Meng","doi":"10.1016/j.jcis.2025.01.183","DOIUrl":"10.1016/j.jcis.2025.01.183","url":null,"abstract":"In photocatalysis, establishing an Ohmic junction could create an internal electric field between semiconductors and cocatalysts [1,2], effectively enhancing the transfer of photogenerated electrons. In this study, the 1T phase dominated oxygen atom doped MoS2 cocatalyst (O-MoS2), synthesized from KSCN molten salt with in-situ oxidation for the first time, is combined with CdS for boosting photocatalytic hydrogen production. In the hybrid photocatalyst, electrons could be efficiently extracted from CdS to O-MoS2 due to the presence of Ohmic contact, thereby significantly enhancing the utilization of photogenerated electrons and the photocatalytic hydrogen evolution performance. The results demonstrate that an initial hydrogen evolution rate of 532.8 μmol-1 could be achieved for CdS with the optimum loading amount of O-MoS2 (CdS-5), 26.6 times higher than that of CdS alone. Additionally, CdS-5 exhibits an apparent quantum yield (AQY) of 80.4 % at 420 nm. The increased photocatalytic performance of CdS-5 is primarily attributed to the efficient electron transfer (ET) process between the CdS and O-MoS2 in the presence of Ohmic junction, which accelerates the separation of the photogenerated carriers from CdS. It is strongly confirmed by the (photo)electrochemical experiments, steady-state/time-resolved photoluminescence (PL) spectra, Kelvin probe force microscope (KPFM), femtosecond transient absorption spectra (fs-TAS) and Density functional theory (DFT) calculation.","PeriodicalId":351,"journal":{"name":"Journal of Colloid and Interface Science","volume":"686 ","pages":"1230-1240"},"PeriodicalIF":9.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced photocatalytic H₂O₂ production via a facile atomic diffusion strategy near tammann temperature for single atom photocatalysts. 通过单原子光催化剂在塔曼温度附近的便捷原子扩散策略提高光催化 H2O2 产率。

IF 9.4 1区化学

Journal of Colloid and Interface Science Pub Date : 2025-05-15 Epub Date: 2025-02-04 DOI: 10.1016/j.jcis.2025.02.014

Tao Zhang, Zhijia Song, Zhiwei Sun, Haichao Li, Zhaoxiong Xie, Qin Kuang

{"title":"Enhanced photocatalytic H2O2 production via a facile atomic diffusion strategy near tammann temperature for single atom photocatalysts.","authors":"Tao Zhang, Zhijia Song, Zhiwei Sun, Haichao Li, Zhaoxiong Xie, Qin Kuang","doi":"10.1016/j.jcis.2025.02.014","DOIUrl":"10.1016/j.jcis.2025.02.014","url":null,"abstract":"Current methods for preparing single atom catalysts (SACs) often suffer from challenges such as high synthesis temperatures, complicated procedures, and expensive equipment. In this study, a facile and universal atomic diffusion strategy near Tamman temperature (AD-TTam) was proposed for the synthesis of semiconductor supported non-noble metal SACs, denoted as M/S, where M = Fe, Ni, Cu, Al and S = ZnO, C3N4, TiO2(A), In2O3. Based on the empirical TTam (c.a. 1/2 of the melting point) phenomenon, this strategy utilized the higher atomic mobility in bulk metals near TTam to facilitate the migration of metal atoms to the support surface, thereby forming SACs at a relatively low temperature. A series of M/S SACs prepared using the AD-TTam strategy all exhibited enhanced photocatalytic H2O2 production activity. Notably, Cu/ZnO achieved an H2O2 production rate of 986.7 μmol g-1h-1 through the synergistic dual pathways of the water oxidation reaction and the oxygen reduction reaction, marking a 5.4-fold increase compared to pure ZnO. The introduction of Cu single atoms significantly improved the separation and migration of charge carriers in Cu/ZnO, thereby promoting the catalytic conversion of H2O and O2. Overall, this strategy is easily extensible at relatively low calcination temperatures and presents great potential for industrial applications.","PeriodicalId":351,"journal":{"name":"Journal of Colloid and Interface Science","volume":"686 ","pages":"1114-1124"},"PeriodicalIF":9.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A double-confined strategy for enhancing the pseudocapacitance performance of nickel-based sulfides-unveiling aqueous pseudocapacitive energy storage mechanism.

IF 9.4 1区化学

Journal of Colloid and Interface Science Pub Date : 2025-05-15 Epub Date: 2025-02-05 DOI: 10.1016/j.jcis.2025.02.027

Gang Yang, Yan Yang, Yan Li, Fangxiang Song, Qianlin Chen

{"title":"A double-confined strategy for enhancing the pseudocapacitance performance of nickel-based sulfides-unveiling aqueous pseudocapacitive energy storage mechanism.","authors":"Gang Yang, Yan Yang, Yan Li, Fangxiang Song, Qianlin Chen","doi":"10.1016/j.jcis.2025.02.027","DOIUrl":"10.1016/j.jcis.2025.02.027","url":null,"abstract":"Lower voltage window and limited pseudocapacitive active sites are identified as critical impediments hindering the advancement of nickel-based supercapacitors. Herein, a double-confined strategy involving nanosizing and heterointerfaces is proposed to construct nickel-based sulfide composite (PMO@NiS2/Ni0.96S@C) with abundant oxygen vacancies (OV), sulfur vacancies (SV), and heterostructures. The composite was prepared using liquid-phase in situ self-assembly and low-temperature in situ induction techniques. The double-confined structure and the introduction of vacancies can effectively expose the pseudocapacitive active sites and improve the operating voltage window range of nickel nanosulfides to enhance pseudocapacitive performance. It is determined that anions and cations in the electrolyte are collectively implicated in the energy storage process. Meanwhile, electrochemical quasi-in situ XPS, in situ electrochemical quartz crystal microbalance (EQCM), and theoretical calculations based on density functional theory (DFT) were utilized to verify the energy storage mechanisms of anions and cations in the electrolyte. Furthermore, a pseudocapacitive reaction mechanism for the composites is proposed, which encompasses a novel charge storage coupling effect between the surface redox reaction of the electrolyte anions and the intercalation/de-intercalation of the electrolyte cations at the interlayer and heterointerface. Consequently, the (PMO@NiS2/Ni0.96S@C) electrode achieves 1807 C/g (6 M KOH, 0.25 A/g) under the working potential window of -0.8 ∼ 0.5 V. The assembled symmetric supercapacitor demonstrates a specific potential of 2 V, yielding an energy density of 96 Wh kg-1 at a power density of 300 W kg-1. This work provides a theoretical reference for designing nickel-based compound materials with high energy density.","PeriodicalId":351,"journal":{"name":"Journal of Colloid and Interface Science","volume":"686 ","pages":"1089-1104"},"PeriodicalIF":9.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic removal of chromium(VI) and tetracycline by porous carbon sponges embedded with MoS₂: Performance and radical mechanism of piezoelectric catalysis.

IF 9.4 1区化学

Journal of Colloid and Interface Science Pub Date : 2025-05-15 Epub Date: 2025-02-06 DOI: 10.1016/j.jcis.2025.02.036

Hongwei Xu, Xiaxia Chen, Chao Liu, Mingyang Xu, Chenxi Guo, Yinglong Wang, Zhaoyou Zhu, Fanqing Meng

{"title":"Synergistic removal of chromium(VI) and tetracycline by porous carbon sponges embedded with MoS2: Performance and radical mechanism of piezoelectric catalysis.","authors":"Hongwei Xu, Xiaxia Chen, Chao Liu, Mingyang Xu, Chenxi Guo, Yinglong Wang, Zhaoyou Zhu, Fanqing Meng","doi":"10.1016/j.jcis.2025.02.036","DOIUrl":"10.1016/j.jcis.2025.02.036","url":null,"abstract":"The presence of hexavalent chromium(Ⅵ) Cr(Ⅵ) and antibiotics in the environment can lead to the formation of combined pollutants that are harmful to ecosystems. To address this problem, we synthesized MoS2 embedded in the porous super-hydrophilic polyurethane (PU) sponge and used weak water flow to drive the piezoelectric catalytic synergistic degradation of Cr(Ⅵ) and tetracycline (TC). In this study, the piezoelectric properties of MoS2/PU were confirmed via piezoresponse force microscopy (PFM) and COMSOL multiphysics calculations. Additionally, dynamics experiments, quenching experiments, electron paramagnetic resonance (EPR) spectroscopy, three-dimensional fluorescence excitation-emission matrix (3D-EEM) spectroscopy, and free radical generation rate analysis were conducted to explore the removal performance and mechanisms of MoS2/PU on the TC-Cr(VI) system. The results indicated that the coexistence of the two pollutants promotes the transfer of charge in MoS2/PU, further combined with O2 to produce H2O2 and continued to produce OH. The removal rates of TC and Cr(VI) in the TC-Cr (VI) mixed system at 30 min were 97 % and 94 %, respectively, which were 1.45 and 1.46 times those of the single TC and single Cr(VI) systems, respectively. Finally, potentially vulnerable sites of TC were calculated and analyzed according to density functional theory (DFT). Our results provide a new strategy for improving the degradation efficiency of antibiotics and Cr(VI) metals.","PeriodicalId":351,"journal":{"name":"Journal of Colloid and Interface Science","volume":"686 ","pages":"1175-1187"},"PeriodicalIF":9.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porous carbon nanosheets integrated with graphene-wrapped CoO and CoNx as efficient bifunctional oxygen electrocatalysts for rechargeable zinc-air batteries.

IF 9.4 1区化学

Journal of Colloid and Interface Science Pub Date : 2025-05-01 Epub Date: 2025-01-21 DOI: 10.1016/j.jcis.2025.01.170

Chang Ma, Binji Zhu, Yue Wang, Shuwen Ma, Jingli Shi, Xiangwu Zhang, Yan Song

{"title":"Porous carbon nanosheets integrated with graphene-wrapped CoO and CoNx as efficient bifunctional oxygen electrocatalysts for rechargeable zinc-air batteries.","authors":"Chang Ma, Binji Zhu, Yue Wang, Shuwen Ma, Jingli Shi, Xiangwu Zhang, Yan Song","doi":"10.1016/j.jcis.2025.01.170","DOIUrl":"10.1016/j.jcis.2025.01.170","url":null,"abstract":"The development of advanced bifunctional oxygen electrocatalysts for the oxygen reduction reactions (ORR) and oxygen evolution reactions (OER) is crucial for the practical application of zinc-air batteries (ZABs). Herein, porous carbon nanosheets integrated with abundant graphene-wrapped CoO and CoNx (CoO/CoNx-C) were successfully fabricated through a simple one-step pyrolysis. With convenient porous channel and large accessible surface, abundant CoO/CoNx species and graphene wrapping structure, CoO/CoNx-C exhibited a half-wave potential of 0.844 V in ORR and an overpotential of 384 mV (@10 mA cm-2) in OER in the alkaline environment and presented a negative shift of 9 mV in ORR after 8000 cycles and positive shift of 19 mV in OER after 2000 cycles. Electrochemical acid-washing and comparison analysis revealed that the ORR activity mainly originated from CoO nanoparticles, while CoNx species were greatly responsible for OER catalysis. Furthermore, the as-prepared CoO/CoNx-C endowed the rechargeable liquid and solid ZABs with superior power density (161 mW cm-2 for liquid ZABs and 137 mW cm-2 for solid ZABs) and long-term stability (stable in 1000 h charge/discharge tests) compared to commercial catalysts. This work provides a feasible strategy for cobalt/carbon hybrid materials as advanced bifunctional electrocatalysts for ZABs.","PeriodicalId":351,"journal":{"name":"Journal of Colloid and Interface Science","volume":"685 ","pages":"793-803"},"PeriodicalIF":9.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MALDI-TOF mass spectrometric immunoassay of multiple tumor biomarkers for non-small cell lung cancer screening. 多种肿瘤生物标志物的MALDI-TOF质谱免疫分析用于非小细胞肺癌筛查。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-06 DOI: 10.1016/j.talanta.2025.127550

Mengchen Wang, Chengxiang Zhu, Nan Feng, Yiran Li, Jiahui Sun, Huangxian Ju

{"title":"MALDI-TOF mass spectrometric immunoassay of multiple tumor biomarkers for non-small cell lung cancer screening.","authors":"Mengchen Wang, Chengxiang Zhu, Nan Feng, Yiran Li, Jiahui Sun, Huangxian Ju","doi":"10.1016/j.talanta.2025.127550","DOIUrl":"10.1016/j.talanta.2025.127550","url":null,"abstract":"Cancer biomarkers have been facing some issues such as poor accuracy and low sensitivity in the early diagnosis of tumors. Utilizing biotin-labelled peptide as a mass tag (MT), this work proposes a high-throughput biosensing strategy for matrix-assisted laser desorption/ionization-time of flight mass spectrometric (MALDI-TOF-MS) immunoassay of multiple lung cancer biomarkers. Due to little required dosage, satisfied stability, high sensitivity and accuracy, this method can achieve off-site centralized signal detection after on-site sample incubation. The proposed approach has been successfully applied for the detection of carcinoembryonic antigen (CEA), carbohydrate antigen199 (CA199), carbohydrate antigen 125 (CA125) and cytokeratin-19-fragment (CY211) in serum samples from various stages of non-small cell lung cancer. Based on the analysis of multiple parameters and pathological results, significant differences in biomarkers are found in serum samples of lung cancer patients at different stages. More importantly, the analysis of multiple tumor biomarkers can improve the accuracy and sensitivity of early diagnosis. Therefore, the multiple immunoassay based on MALDI-TOF MS exhibits exceptional performance in terms of high throughput, little sample dosage, stability and sensitivity.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127550"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Bright luminescent Zn2GeO4:Mn NP/MXene hydrogel-based ECL biosensor for glioblastoma diagnosis" [Talanta 276 (2024) 126214].

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-02-02 DOI: 10.1016/j.talanta.2025.127628

Shuanglin Deng, Wenyan Li, Zhenrun Li, Peilin Wang, Qiang Ma

引用次数: 0

A novel method for detecting genetic biomarkers in blood-based liquid biopsies using surface plasmon resonance imaging and magnetic beads shows promise in cancer diagnosis and monitoring. 一种利用表面等离子体共振成像和磁珠在血液液体活检中检测遗传生物标志物的新方法在癌症诊断和监测中显示出前景。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-07 DOI: 10.1016/j.talanta.2025.127543

Noemi Bellassai, Roberta D'Agata, Elena Giordani, Giovanna Ziccheddu, Roberto Corradini, Giuseppe Spoto

{"title":"A novel method for detecting genetic biomarkers in blood-based liquid biopsies using surface plasmon resonance imaging and magnetic beads shows promise in cancer diagnosis and monitoring.","authors":"Noemi Bellassai, Roberta D'Agata, Elena Giordani, Giovanna Ziccheddu, Roberto Corradini, Giuseppe Spoto","doi":"10.1016/j.talanta.2025.127543","DOIUrl":"10.1016/j.talanta.2025.127543","url":null,"abstract":"Directly detecting biomarkers in liquid biopsy for diagnosis and personalized treatment plays a crucial role in managing cancer relapse and increasing survival rates. Typically, the standard analysis of circulating tumour DNA requires lengthy isolation, extraction, and amplification steps, leading to sample contamination, longer turnaround time and higher assay costs. Surface plasmon resonance is an emerging and promising technology for rapid and real-time dynamic biomarker monitoring in liquid biopsy. Here, we propose a new SPR imaging biosensing approach to detect tumour DNA circulating in the blood of colorectal cancer patients by exploiting the unique properties of superparamagnetic particles. Micrometer beads functionalized with a biotinylated oligonucleotide can directly capture DNA target sequences bearing single-nucleotide variations of KRAS oncogene in human blood plasma. Mutated and wild-type peptide nucleic acid probes immobilized on an SPR gold surface recognize complementary and non-complementary DNA targets by discriminating a single nucleotide mismatch. The new assay allows for detecting p.G13D mutated DNA in buffer and spiked human plasma at attomolar level (down to 300 copies mL-1) with minimal sample manipulation and in just a few microliters. The assay was validated using plasma samples from colorectal cancer patients and healthy donors, by discriminating mutated DNA circulating in patients and wild-type DNA found in healthy blood donors. This feature underscores the potential of the liquid biopsy assay as a valuable tool for the diagnosis and monitoring of cancer.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127543"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent distinguishing flavonoid glycosides against aglycones based on the selective recognization of boric acid-functional Eu(III)-organic framework. 基于硼酸-功能Eu(III)-有机骨架选择性识别的黄酮类苷与苷元的荧光识别。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-07 DOI: 10.1016/j.talanta.2025.127559

Shuyun Zhu, Kunming Sun, Shuyi Liu, Qian Wang, Jiatong Fan, Xian-En Zhao, Guobao Xu

{"title":"Fluorescent distinguishing flavonoid glycosides against aglycones based on the selective recognization of boric acid-functional Eu(III)-organic framework.","authors":"Shuyun Zhu, Kunming Sun, Shuyi Liu, Qian Wang, Jiatong Fan, Xian-En Zhao, Guobao Xu","doi":"10.1016/j.talanta.2025.127559","DOIUrl":"10.1016/j.talanta.2025.127559","url":null,"abstract":"Flavonoid glycosides are formed by dehydration condensation of aglycones and sugar molecules. Therefore, discrimination of flavonoid glycosides from their corresponding aglycones is a challenging task because they contain the same aglycone part in their molecular structures. Herein, boric acid-functional Eu(III)-organic framework (BA-Eu-MOF) was applied to discriminate flavonoid glycosides including baicalin (Bai), wogonoside (Wog), rutin (Rut), puerarin (Pue), quercitrin (Que) and astragalin (Ast) from their corresponding aglycones for the first time. Besides as organic ligand to sensitize the luminescence of Eu3+ through \"antenna\" effect, 5-boronobenzene-1,3 dicarboxylic acid provided recognition site for flavonoid glycosides. Infrared, fluorescence, UV-vis, and mass spectra were used to investigate the recognition reaction between BA-Eu-MOF and flavonoid glycosides. The data indicated that the cis-diols of flavonoid glycosides from sugars covalently bonded to boric acid group to form cyclic boronic esters, which quenched the fluorescence of BA-Eu-MOF at 620 nm through decreasing the intersystem efficiency, inner filter effect and photoelectron transfer. In contrast, aglycones could not alter the fluorescence of BA-Eu-MOF because of no covalent bond between them. This probe exhibited high sensitivity towards flavonoid glycosides with the low detection limits of 3.3 nM, 3.5 nM, 33 nM, 56 nM, 5.1 nM and 5.5 nM for Bai, Que, Wog, Ast, Pue and Rut, respectively. The unique recognition ability of boric acid group enables selective and sensitive detection of flavonoid glycosides without the interference of their corresponding aglycones.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127559"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a cpGFP-based biosensor for enhanced quantification of glycolate production in Escherichia coli. 设计一种基于cpgfp的生物传感器，用于增强大肠杆菌中乙醇酸生产的定量。

IF 5.6 1区化学

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-13 DOI: 10.1016/j.talanta.2025.127529

Qiwei Wang, Zhifen Huang, Sen Ma, Mingxue Ma, Sheng Ye, Si Liu

{"title":"Engineering a cpGFP-based biosensor for enhanced quantification of glycolate production in Escherichia coli.","authors":"Qiwei Wang, Zhifen Huang, Sen Ma, Mingxue Ma, Sheng Ye, Si Liu","doi":"10.1016/j.talanta.2025.127529","DOIUrl":"10.1016/j.talanta.2025.127529","url":null,"abstract":"The growing demand for glycolate, fueled by economic development, requires the advancement of production methods. Escherichia coli (E. coli), a preferred host for glycolate production, has undergone extensive metabolic engineering to improve yield. Developing rapid and precise methods for quantifying glycolate concentration is essential for screening high-yielding strains. Here, we present the engineering of a novel circularly permuted green fluorescent protein (cpGFP)-based glycolate sensor, termed GLYCO. GLYCO exhibits high specificity (minimal interference from other metabolites), stability (no decrease in performance after 15 days at -80 °C), and ease of detection via fluorescence measurement, enabling effective in vitro glycolate quantification. GLYCO spans a quantification range from 10 μM to 1 mM, facilitating effective monitoring of glycolate production in metabolically engineered E. coli strains. This biosensor represents a significant advancement in the metabolic engineering toolkit, facilitating efficient detection and optimization of glycolate production in E. coli, with potential applications in industrial biotechnology.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127529"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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