IF 2.9 3区生物学

Metallomics Pub Date : 2025-05-05 DOI: 10.1093/mtomcs/mfaf011

Frank Förster, Lucie Sauzéat, Christine Ferrier-Pagès, Stéphanie Reynaud, Tom E Sheldrake

{"title":"Redox-sensitive δ65Cu isotopic fractionation in the tissue of the scleractinian coral Stylophora pistillata: a biomarker of holobiont photophysiology following volcanic ash exposure.","authors":"Frank Förster, Lucie Sauzéat, Christine Ferrier-Pagès, Stéphanie Reynaud, Tom E Sheldrake","doi":"10.1093/mtomcs/mfaf011","DOIUrl":"https://doi.org/10.1093/mtomcs/mfaf011","url":null,"abstract":"Volcanic ash is a significant source of micronutrients including iron (Fe), copper (Cu), and zinc (Zn) in oligotrophic tropical waters. These bioactive metals enhance primary productivity, influencing local and global biogeochemical cycles. This study explores how volcanic ash exposure affects trace metal uptake and photophysiological response, and how redox-sensitive metal stable isotope measurements in the tissues of the scleractinian coral Stylophora pistillata can provide crucial information on coral health. Controlled coral culture experiments were conducted in which coral nubbins were exposed to varying intensity and duration of volcanic ash. Throughout the experiment, coral symbionts showed enhanced photosynthetic performance irrespective of intensity or duration of ash exposure. Stable isotopes, such as δ65Cu and δ56Fe, in the coral tissue are marked by systematic variations, not associated with intensity or duration of ash exposure. Instead, we suggest biologically modulated redox-sensitive fractionation associated with ash exposure, linked to the coral host's oxidative stress state. This is evidenced by significant correlations between δ65Cu in coral hosts and photophysiology, with lighter Cu isotope ratios associated with higher photosynthetic performances. Hence, we propose that δ65Cu, and more generally redox-sensitive isotopic ratios (i.e. δ56Fe), in coral hosts serves as an indicator of the physiological state of symbiotic corals.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":"17 5","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12050973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics of metal excretion following different doses of sodium EDTA infusion. 不同剂量EDTA钠输注后金属排泄的药代动力学。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-05-05 DOI: 10.1093/mtomcs/mfaf010

Kathrin Schilling, Francisco Ujueta, Siyue Gao, Will A Anderson, Esteban Escolar, Ana Mon, Ana Navas-Acien, Gervasio A Lamas

{"title":"Pharmacokinetics of metal excretion following different doses of sodium EDTA infusion.","authors":"Kathrin Schilling, Francisco Ujueta, Siyue Gao, Will A Anderson, Esteban Escolar, Ana Mon, Ana Navas-Acien, Gervasio A Lamas","doi":"10.1093/mtomcs/mfaf010","DOIUrl":"https://doi.org/10.1093/mtomcs/mfaf010","url":null,"abstract":"Chelation therapy is a promising approach to mitigating health risks associated with toxic metal exposure, which contributes to cardiovascular disease, neurotoxicity, and other chronic conditions. disodium ethylene diamine tetraacetic acid (EDTA) is widely used, but its optimal dosing strategy remains unclear. This study evaluates the dose-dependent efficacy of EDTA in mobilizing toxic metals, including lead (Pb), cadmium (Cd), and gadolinium (Gd), while minimizing the loss of essential metals like copper (Cu) and manganese (Mn) to optimize therapeutic safety and efficacy. Ten volunteers (≥50 years) received 3 infusions at doses of 0.5, 1, and 3 g of EDTA over 30 min, 1 h, and 3 h, respectively. Urine and blood samples were analyzed pre- and post-infusion to assess pharmacokinetics of metal chelation. Urinary Pb excretion increased by 2200% at 0.5 g, with only a marginal gain at higher doses (3300%), supporting low-dose EDTA efficacy. Urinary Cd clearance required 3 g EDTA due to its strong tissue binding. Notably, Gd excretion increased by up to 78 000% even at 0.5 g EDTA, highlighting EDTA's potential to reduce long-term Gd burden post-MRI. Urinary excretion of essential metals varied, with Mn and Zn loss increasing at higher EDTA doses, underscoring the need for dose optimization while Cu and Ca only showed a clear increase urinary excretion at 3 g EDTA. Overall, a 0.5 g EDTA dose effectively mobilized Pb and Gd while minimizing essential metal depletion, reducing infusion time to 30 min, and improving patient compliance. These findings align with TACT and TACT 2 studies, reinforcing EDTA's long-term benefits in Pb reduction and supporting low-dose EDTA as a safe, efficient, and well-tolerated detoxification strategy.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":"17 5","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12050972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, characterization, DNA interaction studies, and biological evaluation of copper(II) hybrids containing azole drugs and intercalating ligands against neglected diseases. 含唑类药物和嵌入配体的铜（II）杂合体抗被忽视疾病的合成、表征、DNA相互作用研究和生物学评价。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-05-02 DOI: 10.1093/mtomcs/mfaf012

José Aleixo de Azevedo-França, Victor Feliciano Dos Santos Ramos, Patrícia Salvador Tessaro, Heveline Silva, Luigi Messori, Fabio Santanni, Lorenzo Sorace, Luana Pereira Borba-Santos, Sonia Rozental, Juliany Cola Fernandes Rodrigues, Maribel Navarro

{"title":"Synthesis, characterization, DNA interaction studies, and biological evaluation of copper(II) hybrids containing azole drugs and intercalating ligands against neglected diseases.","authors":"José Aleixo de Azevedo-França, Victor Feliciano Dos Santos Ramos, Patrícia Salvador Tessaro, Heveline Silva, Luigi Messori, Fabio Santanni, Lorenzo Sorace, Luana Pereira Borba-Santos, Sonia Rozental, Juliany Cola Fernandes Rodrigues, Maribel Navarro","doi":"10.1093/mtomcs/mfaf012","DOIUrl":"https://doi.org/10.1093/mtomcs/mfaf012","url":null,"abstract":"In an attempt to treat neglected diseases such as leishmaniasis and fungal infections, three novel copper(II) hybrid have been developed: [Cu(dppz)(CTZ)(NO3)](NO3) (1), [Cu(dppz)(KTZ)(H2O)(NO3)](NO3) (2), [Cu(dppz)(FLZ)(NO3)]2(NO3)2 (3). They were synthesized by coordinating antifungal imidazole based drugs and dipyridophenazine as ligands to copper(II) under mild conditions and in good yields. These coordination compounds were characterized by various analytical and spectroscopic techniques which confirmed the coordination of both ligands to the metal, and the monodentate (1) or bidentate (2 and 3) coordination of the nitrate and as counterion. These copper hybrids were stable in solid state, in DMSO and the DMSO-water mixture. DNA interactions were studied using absorption and fluorescence titrations, viscosity measurements, and electrophoresis assays. Complexes 1 and 2 formed strong interaction with DNA. The activity against Leishmania was the highest with complex 3, unlike against S. brasiliensis, where the free imidazole-based drugs (ITZ and KTZ) performed better.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective inhibition of NikA mediated Ni(II) import in E. coli by the Indium(III)-EDTA complex. 铟(III)-EDTA络合物选择性抑制NikA介导的大肠杆菌中Ni（II）的进口。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-03-28 DOI: 10.1093/mtomcs/mfaf008

Stephanie Sebastiampillai, Mark Nitz

{"title":"Selective inhibition of NikA mediated Ni(II) import in E. coli by the Indium(III)-EDTA complex.","authors":"Stephanie Sebastiampillai, Mark Nitz","doi":"10.1093/mtomcs/mfaf008","DOIUrl":"10.1093/mtomcs/mfaf008","url":null,"abstract":"Nickel is a required nutrient for bacteria to produce [NiFe]-hydrogenase and urease enzymes. [NiFe]-hydrogenase catalyzes the reversible conversion of hydrogen into protons and electrons and urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia-both key in bacterial pathogenesis. As such, nickel trafficking and homeostasis are interesting targets for potential antibacterial strategies. In E. coli, NikA binds a Ni(II)-(L-His)2 chelate in the periplasm and delivers this complex to the NikBCDE transporter. Blocking Ni(II) uptake by NikA would prevent the biosynthesis of active [NiFe]-hydrogenase. Fe(III)-EDTA is a potent ligand for NikA, however due to the potential for reduction of Fe(III) to Fe(II), it has limited utility. Using Fe(III)-EDTA as a starting point for inhibitor design, similar stable complexes of Bismuth(III), Lutetium(III) and Indium(III) were investigated. The In(III)-EDTA complex is a potent inhibitor of cellular [NiFe]-hydrogenase activity (IC50 of 600 μM ± 100 μM) while being nontoxic to bacterial growth. The mechanism of In(III)-EDTA hydrogenase inhibition was confirmed by the inhibition of Ni(II)-dependent processing of HycE (hydrogenase-3), which could be rescued with the addition of exogenous nickel. To elucidate the binding affinity of In(III)-EDTA to NikA, isothermal titration calorimetry (ITC) was carried out, revealing stoichiometric 1:1 binding with a Kd of 17.3 µM ± 3.0 µM. Indium concentrations determined by inductively coupled plasma mass spectrometry in E. coli cells in the presence or absence of NikA showed no discernable difference, further supporting the competitive inhibition of nickel uptake by blocking NikA.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlative analysis of metallomic gene expression and metal ion content within the mouse hippocampus. 小鼠海马金属组学基因表达与金属离子含量的相关性分析。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-03-28 DOI: 10.1093/mtomcs/mfaf009

Somayra S A Mamsa, Gaewyn Ellison, Julia Koehn, Keea Inder-Smith, Cameron W Evans, Ross M Graham, Daryl L Howard, Mark J Hackett

{"title":"Correlative analysis of metallomic gene expression and metal ion content within the mouse hippocampus.","authors":"Somayra S A Mamsa, Gaewyn Ellison, Julia Koehn, Keea Inder-Smith, Cameron W Evans, Ross M Graham, Daryl L Howard, Mark J Hackett","doi":"10.1093/mtomcs/mfaf009","DOIUrl":"10.1093/mtomcs/mfaf009","url":null,"abstract":"Brain metal homeostasis is essential for healthy neurological function, and disturbed brain metal homeostasis has deleterious consequences for neurodevelopment or cognitive outcome following injury or during disease. Specific regions of the brain (e.g. the hippocampus and subregions within) are known to be enriched with transition metals (i.e. ions of iron, copper, and zinc). Neither the physiological need for localized enrichment, nor the mechanisms driving the enrichment, however, are well understood. In this study we have applied a multimodal template, incorporating elemental mapping using X-ray fluorescence microscopy with spatial transcriptomics, to help reveal a molecular basis for metallomic heterogeneity across key subregions of the hippocampus. Our results reveal that significant differences in iron, zinc, and copper enrichment are associated with regional enrichment of specific transcripts related to metal transport, metal storage, and metal regulatory proteins. In addition to providing novel biological insight into the neurometallomic profile of the hippocampus, this study also provides an important template for others to integrate transcriptomics into multimodal workflows investigating the neurometallome.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perturbations in levels of essential metals less severe in Parkinson's disease without dementia than in Parkinson's disease dementia. 基本金属水平的扰动在无痴呆的帕金森病中比在帕金森病痴呆中更轻。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-03-07 DOI: 10.1093/mtomcs/mfaf006

Melissa Scholefield, Stephanie J Church, Garth J S Cooper

{"title":"Perturbations in levels of essential metals less severe in Parkinson's disease without dementia than in Parkinson's disease dementia.","authors":"Melissa Scholefield, Stephanie J Church, Garth J S Cooper","doi":"10.1093/mtomcs/mfaf006","DOIUrl":"10.1093/mtomcs/mfaf006","url":null,"abstract":"It is currently unknown why some individuals with Parkinson's disease (PD) go on to develop dementia [Parkinson's disease dementia (PDD)], whereas others do not. One possibility is differences in susceptibility to metallomic dysregulation. A previous study of the PDD brain identified substantive perturbations in metal levels, including severe multiregional decreases in Cu. The current work uses the same methods to ascertain whether this metallomic dysfunction is also present in the PD brain. To do this, tissue from 9 PD cases free of cognitive decline and 15 equivalent controls was obtained from 7 brain regions. Levels of Na, Mg, K, Ca, Mn, Fe, Cu, Zn, and Se were quantified using inductively coupled plasma mass spectrometry (ICP-MS). Multiple linear regression analysis was used to determine any potential confounder effects. Results were compared with those previously obtained for PDD. It was found that decreased Cu in the medulla was the only statistically significant case-control difference observed in the PD brain; this contrasts markedly with the widespread metallic dysfunction observed in PDD. PD and PDD cases were well separated by PCA analysis. In the PD cohort, tau Braak stage correlated with Cu concentrations in several regions, but these correlations were not retained when including PDD cases. There is a marked difference in the metallomic profiles of PD and PDD, with an almost complete lack of metallic involvement observed in the former. This resistance to metallomic dysfunction may contribute to resilience against cognitive impairment in individuals with PD who do not develop dementia.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11895508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the origin of metal species in the human brain: a perspective on key physicochemical properties. 人脑中金属种类的起源：从关键物理化学性质的角度看。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-03-07 DOI: 10.1093/mtomcs/mfaf004

Jake Brooks, James Everett, Peter J Sadler, Neil Telling, Joanna F Collingwood

引用次数: 0

Time-resolved proteomic profiling of Cupriavidus metallidurans CH34 in the copper-induced viable-but-nonculturable state. 铜诱导的活但不可培养状态下金属铜球菌CH34的时间分辨蛋白质组学分析。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-03-07 DOI: 10.1093/mtomcs/mfaf007

Timothej Patocka, Surya Gupta, Felice Mastroleo, Natalie Leys, Jean-Yves Matroule, Rob Van Houdt

{"title":"Time-resolved proteomic profiling of Cupriavidus metallidurans CH34 in the copper-induced viable-but-nonculturable state.","authors":"Timothej Patocka, Surya Gupta, Felice Mastroleo, Natalie Leys, Jean-Yves Matroule, Rob Van Houdt","doi":"10.1093/mtomcs/mfaf007","DOIUrl":"10.1093/mtomcs/mfaf007","url":null,"abstract":"Copper-based materials are actively explored for their potential as antimicrobial agents. However, recent studies show that sublethal concentrations of Cu ions can induce the viable-but-nonculturable (VBNC) cell state in certain bacteria, hampering contamination control, and monitoring. In this study we contribute to the unravelling of this largely enigmatic phenomenon by determining the time-resolved proteome of Cu-treated Cupriavidus metallidurans CH34 during VBNC induction and resuscitation. High-throughput quantitative liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis was performed at multiple sample time points, revealing the cellular adaptations that trigger VBNC formation and the characteristic spontaneous recovery of culturability. Entry into the VBNC state correlated with a widespread response to oxidative stress as well as downregulated pyruvate metabolism. The expression of specific metal resistance determinants changed with Cu exposure time and culminated in the strong upregulation of proteins linked to periplasmic Cu ion detoxification during the resuscitation phase. We suggest that this delayed induction of Cu resistance proteins is paralleled by the gradual reconstitution of energy reserves through metabolization of intracellular polyhydroxybutyrate, as supported by flow cytometric fluorescence measurements. Furthermore, Cu-treated cells showed upregulation of several motility and chemotaxis proteins, and increased cell motility was observed phenotypically. Our results reveal a highly dynamic proteomic response, provide fundamental insights into the VBNC state and emphasize the advantages of time-resolved proteomic analysis.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11886801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synchrotron-based correlative imaging of metals and proteins in neuronal cells: state of the art and future challenges in neurometallomics. 基于同步加速器的神经细胞中金属和蛋白质的相关成像：神经金属组学的现状和未来挑战。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-02-06 DOI: 10.1093/mtomcs/mfaf003

Ines Kelkoul, Virginia Puente Muñoz, Richard Ortega, Asuncion Carmona

{"title":"Synchrotron-based correlative imaging of metals and proteins in neuronal cells: state of the art and future challenges in neurometallomics.","authors":"Ines Kelkoul, Virginia Puente Muñoz, Richard Ortega, Asuncion Carmona","doi":"10.1093/mtomcs/mfaf003","DOIUrl":"10.1093/mtomcs/mfaf003","url":null,"abstract":"Metal homeostasis in the nervous system is subtly regulated and changes in metal distribution or content, either increases or decreases, are associated with neurodegeneration or cognitive impairment. Determining the localization and quantification of metals in different types of neurons is important information for understanding their role in neurobiology. Synchrotron X-ray fluorescence imaging is a powerful technique that provides very high sensitivity and high spatial resolution for imaging metals in cells. However, additional biological information is often required to correlate the subcellular localization of metals with specific proteins or organelles. The purpose of this article is to review the studies in neuroscience that correlate metal imaging by synchrotron X-ray fluorescence with protein localization by other techniques. This article highlights the diversity of correlative modalities that have been used, from fluorescence to super-resolution and infrared microscopy, and the wealth of information that has been extracted, but also discusses some current limitations. Future developments are needed, particularly for direct imaging of metals and proteins with a single instrument.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of laser-ablation inductively-coupled plasma mass spectrometry for analysis of selenosugars bound to proteins. 用激光烧蚀电感耦合质谱法分析与蛋白质结合的硒糖。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-02-06 DOI: 10.1093/mtomcs/mfaf002

Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski, Roger A Sunde

{"title":"Use of laser-ablation inductively-coupled plasma mass spectrometry for analysis of selenosugars bound to proteins.","authors":"Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski, Roger A Sunde","doi":"10.1093/mtomcs/mfaf002","DOIUrl":"10.1093/mtomcs/mfaf002","url":null,"abstract":"We previously used high pressure liquid chromatography coupled with Se-specific inductively coupled plasma mass spectrometry and molecule specific (ESI Orbitrap MS/MS) detection to study the increase in liver Se in turkeys and rats supplemented as selenite in high-Se (5 µg Se/g diet) and adequate-Se diets. We found that far more Se is present as selenosugar (seleno-N-acetyl galactosamine) than is present as selenocysteine (Sec) in true selenoproteins. In high-Se liver, the increase in liver Se was due to low molecular weight selenometabolites such as glutathione-, cysteine-, and methyl-conjugates of the selenosugar, but also as high molecular weight species as selenosugars decorating general proteins via mixed Se-S bonds. To demonstrate selenosugar binding to proteins, aqueous liver extracts from animals fed Se-adequate and high-Se were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Native-PAGE with and without pretreatment with β-mercaptoethanol (βME). The separated proteins were then electrophoretically transferred to membranes, and the membranes subsequently were subjected to laser-ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS) analysis of 78Se profiles. Without βME treatment, Se was widely distributed across the molecular weight profile for both SDS-PAGE and Native-PAGE, whereas βME pretreatment dramatically reduced Se binding, reducing the profile to true Sec-selenoproteins. This reduction was ∼50% for both high-Se rat and turkey extracts. The increased Se in non-βME treated samples was distributed across the full profile. The use of LA-ICP-MS indicates that selenosugar residues are bound to protein subunits of multiple sizes, and that targeted attachment of selenosugars to a single or limited number of protein subunits does not occur.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metallomics最新文献