IF 2.9 3区生物学

Metallomics Pub Date : 2025-10-01 DOI: 10.1093/mtomcs/mfaf035

Haiyan Yuan, Zhijie Duan, Gang Liang

{"title":"Genome-wide identification and expression analysis of yellow stripe-like (YSL) genes in Nicotiana tabacum.","authors":"Haiyan Yuan, Zhijie Duan, Gang Liang","doi":"10.1093/mtomcs/mfaf035","DOIUrl":"https://doi.org/10.1093/mtomcs/mfaf035","url":null,"abstract":"Iron (Fe) and copper (Cu) are crucial micronutrients for plant growth and development. The yellow stripe-like (YSL) proteins play a vital role in the absorption and transport of metal chelates in plants. The YSL genes in tobacco have not been systematically identified and characterized. This study aims to explore the YSL genes in Nicotiana tabacum. A comprehensive set of 17 NtYSL genes were identified and classified into four distinct clades on the phylogenetic tree. The gene structures, characterized by the length and distribution of exons and introns, and protein motifs are relatively conserved. Genomic localization analysis revealed that the NtYSL genes are unevenly distributed across 15 chromosomes, with 11 pairs of homeologous loci identified within the genome. To investigate the functionality of these genes, we analyzed their expression levels in shoots and roots under Fe- and Cu-deficient conditions by real-time quantitative reverse transcription PCR (qRT-PCR), finding that several NtYSL genes are responsive to Cu deficiency or Fe deficiency. This study provides a systematic characterization of the NtYSL gene family in Nicotiana tabacum and offers insights into their potential roles in Fe and Cu homeostasis.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monoribbed-functionalized iron(II) clathrochelates with optically active and/or terminal biorelevant group(s): synthesis, single-crystal X-ray diffraction and quantum-chemical characterization, and their inherent versus protein-induced chirality. 具有光学活性和/或末端生物相关基团的单功能化铁（II）螯合物：合成、单晶x射线衍射和量子化学表征，及其固有的与蛋白质诱导的手性。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-09-27 DOI: 10.1093/mtomcs/mfaf033

Ilya P Limarev, Alexander S Belov, Alexander L Pomadchik, Yurii V Fedorov, Yan V Zubavichus, Anna V Vologzhanina, Alexander A Korlyukov, Yan Z Voloshin

{"title":"Monoribbed-functionalized iron(II) clathrochelates with optically active and/or terminal biorelevant group(s): synthesis, single-crystal X-ray diffraction and quantum-chemical characterization, and their inherent versus protein-induced chirality.","authors":"Ilya P Limarev, Alexander S Belov, Alexander L Pomadchik, Yurii V Fedorov, Yan V Zubavichus, Anna V Vologzhanina, Alexander A Korlyukov, Yan Z Voloshin","doi":"10.1093/mtomcs/mfaf033","DOIUrl":"https://doi.org/10.1093/mtomcs/mfaf033","url":null,"abstract":"Novel monoribbed-functionalized iron(II) cage complexes with optically active and/or terminal biorelevant group(s) were designed and prepared by two-step nucleophilic substitution of their mono- and dichloroclathrochelate precursors. The single-crystal XRD structures of all of them and those of known leader iron(II)-centered cage bioeffector and of its reactive monochloroclathrochelate precursor were solved. These experimental data were used for theoretical quantum-chemical calculations of electrostatic potentials for their 3D-shaped molecules. This allowed to localize the peripheral (exterior) biorelevant group(s), which are responsible for supramolecular binding of thus designed clathrochelate guests to globular proteins as the hosts. Host-guest binding in aqueous solutions between the unfolded protein macromolecules and all the aforementioned iron(II) complexes was studied by the circular dichroism method. An inherent chirality of the metalloclathrochelates with optically active ribbed substituent and a metal-centered chirality of all the prepared macrobicyclic compounds, induced by their supramolecular clathrochelate-to-protein binding, were observed.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interplay of iron status and manganese biomarkers in U.S. females: NHANES 2015-2018 analysis. 美国女性铁状态和锰生物标志物的相互作用：NHANES 2015-2018分析

IF 2.9 3区生物学

Metallomics Pub Date : 2025-09-23 DOI: 10.1093/mtomcs/mfaf032

Yu-Sheng Lin, James L Caffrey, Babasaheb Sonawane, Miyoung Yoon, Viktor Morozov, Wen-Chao Ho

{"title":"The interplay of iron status and manganese biomarkers in U.S. females: NHANES 2015-2018 analysis.","authors":"Yu-Sheng Lin, James L Caffrey, Babasaheb Sonawane, Miyoung Yoon, Viktor Morozov, Wen-Chao Ho","doi":"10.1093/mtomcs/mfaf032","DOIUrl":"10.1093/mtomcs/mfaf032","url":null,"abstract":"Manganese (Mn) plays a dual role in the body, acting as an essential trace element and a potential toxicant, the effects of which depend on its levels. In addition to food, exposure can occur through polluted air and contaminated water. Animal studies suggest that increased retention and absorption of Mn might result from iron deficiency, as both share similar physicochemical properties. However, human evidence is incomplete. This study aimed to confirm and expand upon prior findings that iron status influences Mn kinetics in the U.S. female population. The analysis included 1255 non-pregnant females aged 12-49 years with valid urinary and blood Mn and iron measurements as part of the 2015-2018 National Health and Nutrition Examination Survey. Iron status was assessed with a total body iron (TBI) score calculated from measured serum ferritin and the transferrin receptor. Iron deficiency was defined as a TBI score < 0. Demographic and laboratory characteristics (e.g. age and kidney function) were recorded. Among the study participants, roughly 8.8% were found to have iron deficiency. Conversely, 16.9% of participants exhibited blood Mn levels exceeding 1.5 µg/dL, a commonly used reference. On average, blood Mn was approximately 40% higher in subjects considered iron deficient than in their counterparts after controlling for covariates such as race. Those with iron deficiency also had a lower urine-to-blood Mn ratio. The findings suggest that iron-deficient females may have greater Mn accumulation, increasing the risk of Mn toxicity. Further investigations should include male populations to complement the current findings.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interactome of the Bakers' yeast peroxiredoxin Tsa1 implicates it in redox regulation of intermediary metabolism, glycolysis, and zinc homeostasis. 面包师酵母过氧化物还原素Tsa1的相互作用表明它参与了中间代谢、糖酵解和锌稳态的氧化还原调节。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-08-05 DOI: 10.1093/mtomcs/mfaf028

Colin W MacDiarmid, Yirong Wang, Janet Taggart, Ajay Vashisht, Xin Qing, James A Wohlschlegel, David J Eide

{"title":"The interactome of the Bakers' yeast peroxiredoxin Tsa1 implicates it in redox regulation of intermediary metabolism, glycolysis, and zinc homeostasis.","authors":"Colin W MacDiarmid, Yirong Wang, Janet Taggart, Ajay Vashisht, Xin Qing, James A Wohlschlegel, David J Eide","doi":"10.1093/mtomcs/mfaf028","DOIUrl":"10.1093/mtomcs/mfaf028","url":null,"abstract":"Zinc (Zn) is an essential nutrient supporting a range of critical processes. In the yeast Saccharomyces cerevisiae, Zn deficiency induces a transcriptional response mediated by the Zap1 activator, which controls a regulon of ∼80 genes. A subset support Zn homeostasis by promoting Zn uptake and its distribution between compartments, while the remainder mediate an 'adaptive response' to enhance fitness of Zn-deficient (ZnD) cells. The peroxiredoxin Tsa1 is a Zap1-regulated adaptive factor essential for the growth of ZnD yeast. Tsa1 can function as an antioxidant peroxidase, protein chaperone, or redox sensor: The latter activity oxidizes associated proteins via a redox relay mechanism. We previously reported that in ZnD cells, Tsa1 inhibits pyruvate kinase (Pyk1) to conserve phosphoenolpyruvate for aromatic amino acid synthesis. However, this regulation makes a relatively minor contribution to fitness in low Zn, suggesting that Tsa1 targets other pathways important to adaptation. Consistent with this model, the redox sensor function of Tsa1 was essential for growth of ZnD cells. Using a maltose binding protein-tagged version of Tsa1, we identified a redox-sensitive non-covalent interaction with Pyk1, and applied this system to identify multiple novel interacting partners. This interactome implicates Tsa1 in the regulation of critical processes including many Zn-dependent metabolic pathways. Interestingly, Zap1 is a Tsa1 target, as Tsa1 strongly promoted the oxidation of Zap1 activation domain 2 and was required for full Zap1 activity. Our findings reveal a novel posttranslational response to Zn deficiency, overlain on and interconnected with the Zap1-mediated transcriptional response.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12368497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a mechanistic connection between persulfide transfer and ATP hydrolysis in the SufBC2D scaffold of the Suf Fe-S cluster assembly pathway. Suf Fe-S簇组装途径中SufBC2D支架中过硫转移与ATP水解之间的机制联系的表征。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-08-05 DOI: 10.1093/mtomcs/mfaf029

Nidhi Chhikara, Grishma Timilsina, Yu Wang, Dexter Reasons, F Wayne Outten, Patrick A Frantom

{"title":"Characterization of a mechanistic connection between persulfide transfer and ATP hydrolysis in the SufBC2D scaffold of the Suf Fe-S cluster assembly pathway.","authors":"Nidhi Chhikara, Grishma Timilsina, Yu Wang, Dexter Reasons, F Wayne Outten, Patrick A Frantom","doi":"10.1093/mtomcs/mfaf029","DOIUrl":"10.1093/mtomcs/mfaf029","url":null,"abstract":"The Suf pathway is the most common pathway for bacterial iron-sulfur cluster assembly and uses the SufBC2D complex as a scaffold for cluster formation. In most Gram-negative bacteria, the SufB subunit of SufBC2D accepts a persulfide from the transpersulfurase, SufE, for incorporation into nascent clusters. There is no reported structure for the SufBC2D-E complex and mechanistic details concerning the coordination of persulfide delivery with other SufBC2D activities are unclear. Using the Suf pathway from Escherichia coli as a model system, we report that SufE acts as a noncompetitive inhibitor of SufBC2D ATPase activity with a Ki value of 1.8 ± 0.2 µM. This value corresponds with a KD value of 1.6 ± 0.2 µM for SufE binding to the SufBC2D complex determined by fluorescence polarization. The rate of persulfide transfer from SufE to SufBC2D is impaired in the presence of ATP, suggesting that the two reactions are mutually exclusive. An AlphaFold3 model of the SufBC2D-E complex predicts electrostatic interactions between acidic residues on SufC and basic residues on the N-terminal helix of SufE. SufE variants at the K9 and R16 positions interfere with the ability of SufE to transfer persulfide to SufBC2D and to inhibit SufBC2D ATPase activity. In vivo complementation growth assays show that these SufE variants exhibit a slow-growth phenotype under iron starvation conditions, confirming the connection between SufE and SufC as important for optimal function in the Suf pathway. The mutual exclusivity of persulfide delivery from SufE and SufBC2D ATPase activity suggests an ordered mechanism for cluster assembly.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between copper metabolism and p53 activity in tumors. 肿瘤中铜代谢与p53活性的关系。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-08-05 DOI: 10.1093/mtomcs/mfaf025

Wenyan Yu, Xinlin Hong, Guojuan Wang, Chen Zhong, Yuwei Yan, Qingqing Ma, Yujuan Lai, Naicheng Zhu, Xiudan Chen, Nanxin Li, Jianfeng Weng

引用次数: 0

ArnR binds a [4Fe-4S] cluster and indirectly senses anaerobicity in Corynebacteria. 在棒状菌中，ArnR结合[4Fe-4S]簇并间接感知厌氧。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-08-05 DOI: 10.1093/mtomcs/mfaf026

Jason C Crack, Lauren R Harvey, Katie E Johnson, Nick E Le Brun

{"title":"ArnR binds a [4Fe-4S] cluster and indirectly senses anaerobicity in Corynebacteria.","authors":"Jason C Crack, Lauren R Harvey, Katie E Johnson, Nick E Le Brun","doi":"10.1093/mtomcs/mfaf026","DOIUrl":"10.1093/mtomcs/mfaf026","url":null,"abstract":"Corynebacteria are commercially and medically important Gram-positive bacteria that can switch from aerobic to anaerobic respiration in response to low O2 and the availability of nitrate as an alternative electron acceptor. The narKGHJI operon encoding the respiratory nitrate reductase is under the control of a novel regulator, ArnR, which plays a major role in the aerobic/anaerobic respiratory switch. ArnR was previously shown to be an iron-sulfur cluster protein that modulates its DNA binding according to availability of O2. However, previous data suggest that it does not do this directly in response to O2, but instead by sensing nitric oxide (NO), which builds up only under low O2 through the activity of nitrate reductase. Here, we report spectroscopic and mass spectrometric studies of C. glutamicum ArnR and its reactions with O2 and NO. We demonstrate that ArnR is a dimer that binds a [4Fe-4S] cluster in each subunit, and this form of the protein binds tightly to DNA. The [4Fe-4S] cluster of AnrR degrades only very slowly in the presence of O2, consistent with the ability of ArnR to repress nar transcription under aerobic conditions. Reaction with NO results in the formation of mono- and di-nitrosylated forms of the [4Fe-4S] ArnR dimer, which exhibit altered DNA-binding characteristics such that the di-nitrosyl form no longer binds to promoter DNA (i.e. cluster degradation is not required in order to modulate DNA binding). These data are consistent with previous literature and lead us to propose a model for AnrR regulatory function.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12343034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined stress of Pb and Cd toxic effects on physiological response and potentially toxic elements accumulation characteristics in Cunninghamia lanceolata seedlings. 铅镉复合胁迫对杉木幼苗生理反应及潜在有毒元素（pte）积累特性的影响

IF 2.9 3区生物学

Metallomics Pub Date : 2025-08-05 DOI: 10.1093/mtomcs/mfaf027

Chengfeng Liu, Lita Yi, Zhiwei Ge, Meihua Liu

{"title":"Combined stress of Pb and Cd toxic effects on physiological response and potentially toxic elements accumulation characteristics in Cunninghamia lanceolata seedlings.","authors":"Chengfeng Liu, Lita Yi, Zhiwei Ge, Meihua Liu","doi":"10.1093/mtomcs/mfaf027","DOIUrl":"10.1093/mtomcs/mfaf027","url":null,"abstract":"The extensive contamination of terrestrial ecosystems with multiple potentially toxic elements (PTEs) necessitates elucidation of plant adaptive mechanisms under combined PTEs stress. This study examines the physiological adaptations, antioxidant regulation, and PTEs allocation patterns in Cunninghamia lanceolata seedlings exposed to lead (Pb) stress (Pb4, 4.0 mg kg-1 Pb; Pb40, 40 mg kg-1 Pb), cadmium (Cd) stress (Cd2, 2 mg kg-1 Cd; Cd20, 20 mg kg-1 Cd), and combined Pb and Cd stress. Results demonstrated concentration-dependent inhibition of biomass production and chlorophyll b biosynthesis under both single and combined PTEs stress conditions. Different responses in superoxide dismutase activity were observed under combined stress compared to the controls, with lower concentration Pb stress causing notably higher enzymatic activation compared to higher concentration Pb stress. Elevated Cd concentrations resulted in significant accumulation of malondialdehyde in leaf tissues, indicating membrane damage. Lead preferentially accumulated in leaves under single Pb stress, while Cd predominantly accumulated in root systems. However, when the plants were exposed to combined Pb and Cd stress, the PTEs translocation pathways in the plants were altered, which resulted in a greater retention of Cd in the stems compared to when the plants were exposed to the single PTE stress. These findings provide insights into species-specific PTE homeostasis mechanisms under polymetallic stress, thereby providing theoretical foundations for the development of phytoremediation strategies in environments contaminated with multiple PTEs.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interplay of ferroptosis and oxidative stress mechanisms: a critical contributor to chronic obstructive pulmonary disease pathophysiology. 铁下沉和氧化应激机制的相互作用：慢性阻塞性肺疾病（COPD）病理生理的关键因素。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-08-05 DOI: 10.1093/mtomcs/mfaf030

Vrunda Tavkar, Ankita Goyal, Heena Kansal, Vishal Chopra, Kranti Garg, Siddharth Sharma

{"title":"The interplay of ferroptosis and oxidative stress mechanisms: a critical contributor to chronic obstructive pulmonary disease pathophysiology.","authors":"Vrunda Tavkar, Ankita Goyal, Heena Kansal, Vishal Chopra, Kranti Garg, Siddharth Sharma","doi":"10.1093/mtomcs/mfaf030","DOIUrl":"10.1093/mtomcs/mfaf030","url":null,"abstract":"Ferroptosis, a recently discovered iron-dependent regulated form of cell death, is characterised by lipid peroxidation and oxidative stress. Recent studies suggested that ferroptosis plays a pivotal role in the pathogenesis of chronic obstructive pulmonary disease (COPD), a progressive and irreversible lung disorder, marked by airflow limitation, emphysema, and chronic bronchitis. Cigarette smoke (CS), one of the prominent risk factors for COPD, is known to induce ferroptosis by generating reactive oxygen species (ROS), depleting antioxidant defences, such as glutathione and glutathione peroxidase 4, and disrupting iron homeostasis. These molecular disturbances lead to cell damage, alveolar destruction, and vascular dysfunction, contributing to disease progression and exacerbations. Ferroptosis is also linked with key COPD mechanisms, which are responsible for mitochondrial dysfunction, inflammation, pulmonary hypertension, and CS-induced irregular distribution of iron-binding proteins. A promising therapeutic strategy for mitigating COPD pathogenesis is targeting ferroptosis via iron chelators, lipid peroxide inhibitors, and antioxidant upregulation. Understanding the regulatory mechanisms governing ferroptosis in lung tissue damage could help identify novel biomarkers and effective treatment strategies. This review explores the mechanistic role of ferroptosis in COPD and uncovers the potential intervention methods that may improve clinical outcomes.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic profiling of zinc homeostasis mechanisms in Pseudomonas aeruginosa through data-dependent and data-independent acquisition mass spectrometry. 铜绿假单胞菌锌稳态机制的蛋白质组学分析——数据依赖性和数据非依赖性获取质谱分析。

IF 2.9 3区生物学

Metallomics Pub Date : 2025-08-05 DOI: 10.1093/mtomcs/mfaf020

Annaliese C S Meyer, Matthew R McIlvin, Paloma Lopez, Brian C Searle, Mak A Saito

{"title":"Proteomic profiling of zinc homeostasis mechanisms in Pseudomonas aeruginosa through data-dependent and data-independent acquisition mass spectrometry.","authors":"Annaliese C S Meyer, Matthew R McIlvin, Paloma Lopez, Brian C Searle, Mak A Saito","doi":"10.1093/mtomcs/mfaf020","DOIUrl":"10.1093/mtomcs/mfaf020","url":null,"abstract":"Zinc is central to the function of many proteins, yet the mechanisms of zinc homeostasis and their interplay with other cellular systems remain underexplored. In this study, we employ data-dependent acquisition (DDA) and data-independent acquisition (DIA) mass spectrometry to investigate proteome changes in Pseudomonas aeruginosa under conditions of different zinc availability. Using both methods, we detected a combined 2143 unique proteins, 1578 of which were identified by both DDA and DIA. We demonstrated that most of the previously described Zn homeostasis systems exhibit proteomic responses that follow similar trends to those seen in transcriptomics studies. Furthermore, changes in abundance of multiple Zn-metalloproteins and Zn-independent homologs were clearly observable, with respective increases and decreases when Zn was provided, though the magnitude of these changes varied. Most of the Zn-metalloproteins observed were located in one of two Zur-regulated operons between PA5534 and PA5541. This study provides a view of Zn homeostasis mechanisms that is complementary to existing transcriptomics investigations: as gene transcripts are not strictly proportional to the actual distribution of proteins within a cell, analysis of the proteome offers another way to assess the relative use and importance of similar or ostensibly redundant systems in different conditions and can highlight shifts in metal prioritization between metalloproteins.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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