烟草黄条样基因的全基因组鉴定与表达分析。

IF 2.9 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Metallomics Pub Date : 2025-10-01 DOI:10.1093/mtomcs/mfaf035
Haiyan Yuan, Zhijie Duan, Gang Liang
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引用次数: 0

摘要

铁(Fe)和铜(Cu)是植物生长发育的重要微量元素。黄色条纹样蛋白(YSL)在植物金属螯合物的吸收和运输中起着至关重要的作用。烟草中YSL基因尚未被系统地鉴定和表征。本研究旨在探索烟草中YSL基因。在系统发育树上,共鉴定出17个ntsl基因,并将其划分为4个不同的支系。以外显子和内含子的长度和分布为特征的基因结构和蛋白质基序相对保守。基因组定位分析显示,ntsl基因在15条染色体上分布不均匀,在基因组内鉴定出11对同源位点。为了研究这些基因的功能,我们利用实时定量反转录PCR (qRT-PCR)分析了它们在缺铁和缺铜条件下在茎和根中的表达水平,发现一些ntsl基因对缺铜或缺铁有反应。本研究提供了烟草nttysl基因家族的系统特征,并为其在铁和铜稳态中的潜在作用提供了见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome-wide identification and expression analysis of yellow stripe-like (YSL) genes in Nicotiana tabacum.

Iron (Fe) and copper (Cu) are crucial micronutrients for plant growth and development. The yellow stripe-like (YSL) proteins play a vital role in the absorption and transport of metal chelates in plants. The YSL genes in tobacco have not been systematically identified and characterized. This study aims to explore the YSL genes in Nicotiana tabacum. A comprehensive set of 17 NtYSL genes were identified and classified into four distinct clades on the phylogenetic tree. The gene structures, characterized by the length and distribution of exons and introns, and protein motifs are relatively conserved. Genomic localization analysis revealed that the NtYSL genes are unevenly distributed across 15 chromosomes, with 11 pairs of homeologous loci identified within the genome. To investigate the functionality of these genes, we analyzed their expression levels in shoots and roots under Fe- and Cu-deficient conditions by real-time quantitative reverse transcription PCR (qRT-PCR), finding that several NtYSL genes are responsive to Cu deficiency or Fe deficiency. This study provides a systematic characterization of the NtYSL gene family in Nicotiana tabacum and offers insights into their potential roles in Fe and Cu homeostasis.

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来源期刊
Metallomics
Metallomics 生物-生化与分子生物学
CiteScore
7.00
自引率
5.90%
发文量
87
审稿时长
1 months
期刊介绍: Global approaches to metals in the biosciences
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