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Ultradense Array of On-Chip Sensors for High-Throughput Electrochemical Analyses. 用于高通量电化学分析的超密集片上传感器阵列
IF 8.2 1区 化学
ACS Sensors Pub Date : 2024-07-12 DOI: 10.1021/acssensors.4c01026
Lucas B Ayres, Gabriel J C Pimentel, Juliana N Y Costa, Maria H O Piazzetta, Angelo L Gobbi, Flávio M Shimizu, Carlos D Garcia, Renato S Lima
{"title":"Ultradense Array of On-Chip Sensors for High-Throughput Electrochemical Analyses.","authors":"Lucas B Ayres, Gabriel J C Pimentel, Juliana N Y Costa, Maria H O Piazzetta, Angelo L Gobbi, Flávio M Shimizu, Carlos D Garcia, Renato S Lima","doi":"10.1021/acssensors.4c01026","DOIUrl":"https://doi.org/10.1021/acssensors.4c01026","url":null,"abstract":"<p><p>High-throughput sensors are valuable tools for enabling massive, fast, and accurate diagnostics. To yield this type of electrochemical device in a simple and low-cost way, high-density arrays of vertical gold thin-film microelectrode-based sensors are demonstrated, leading to the rapid and serial interrogation of dozens of samples (10 μL droplets). Based on 16 working ultramicroelectrodes (UMEs) and 3 quasi-reference electrodes (QREs), a total of 48 sensors were engineered in a 3D crossbar arrangement that devised a low number of conductive lines. By exploiting this design, a compact chip (75 × 35 mm) can enable performing 16 sequential analyses without intersensor interferences by dropping one sample per UME finger. In practice, the electrical connection to the sensors was achieved by simply switching the contact among WE adjacent fingers. Importantly, a short analysis time was ensured by interrogating the UMEs with chronoamperometry or square wave voltammetry using a low-cost and hand-held one-channel potentiostat. As a proof of concept, the detection of <i>Staphylococcus aureus</i> in 15 samples was performed within 14 min (20 min incubation and 225 s reading). Additionally, the implementation of peptide-tethered immunosensors in these chips allowed the screening of COVID-19 from patient serum samples with 100% accuracy. Our experiments also revealed that dispensing additional droplets on the array (in certain patterns) results in the overestimation of the faradaic current signals, a phenomenon referred to as crosstalk. To address this interference, a set of analyses was conducted to design a corrective strategy that boosted the testing capacity by allowing using all on-chip sensors to address subsequent analyses (i.e., 48 samples simultaneously dispensed on the chip). This strategy only required grounding the unused rows of QRE and can be broadly adopted to develop high-throughput UME-based sensors. In practice, we could analyze 48 droplets (with [Fe(CN)<sub>6</sub>]<sup>4<i>-</i></sup>) within ∼8 min using amperometry.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":null,"pages":null},"PeriodicalIF":8.2,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141597876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Visible Light-Activated Room Temperature NO2 Gas Sensing Based on the In2O3@ZnO Heterostructure with a Hollow Microtube Structure. 基于具有空心微管结构的 In2O3@ZnO 异质结构的可见光激活型室温二氧化氮气体传感。
IF 8.2 1区 化学
ACS Sensors Pub Date : 2024-07-12 DOI: 10.1021/acssensors.4c00919
Ying Li, Xiangyang Wei, Qingyuan Liu, Diming Zang, Rui You
{"title":"Visible Light-Activated Room Temperature NO<sub>2</sub> Gas Sensing Based on the In<sub>2</sub>O<sub>3</sub>@ZnO Heterostructure with a Hollow Microtube Structure.","authors":"Ying Li, Xiangyang Wei, Qingyuan Liu, Diming Zang, Rui You","doi":"10.1021/acssensors.4c00919","DOIUrl":"https://doi.org/10.1021/acssensors.4c00919","url":null,"abstract":"<p><p>The persistent challenge of poor recovery characteristics of NO<sub>2</sub> sensors operated at room temperature remains significant. However, the development of In<sub>2</sub>O<sub>3</sub>-based gas sensing materials provides a promising approach to accelerate response and recovery for sub-ppm of NO<sub>2</sub> detection at room temperature. Herein, we propose a simple two-step method to synthesize a one-dimensional (1D) In<sub>2</sub>O<sub>3</sub>@ZnO heterostructure material with hollow microtubes, by coupling metal-organic frameworks (MOFs) (MIL-68 (In)) and zinc ions. Meanwhile, the In<sub>2</sub>O<sub>3</sub>@ZnO composite-based gas sensor exhibits superior sensitivity performance to NO<sub>2</sub> under visible light activation. The response value to 5 ppm of NO<sub>2</sub> at room temperature is as high as 1800, which is 35 times higher than that of the pure In<sub>2</sub>O<sub>3</sub>-based sensor. Additionally, the gas sensor based on the In<sub>2</sub>O<sub>3</sub>@ZnO heterostructure demonstrates a significantly reduced response/recovery time of 30 s/67 s compared to the sensor based on pure In<sub>2</sub>O<sub>3</sub> (74 s/235 s). The outstanding gas sensing properties of the In<sub>2</sub>O<sub>3</sub>@ZnO heterostructure-based sensors can be attributed to the enhanced photogenerated charge separation efficiency resulting from the heterostructure effect, and the improved receptor function toward NO<sub>2</sub>, which can increase the reactive sites and gas adsorption capacity. In summary, this work proposes a low-cost and efficient method to synthesize a 1D heterostructure material with microtube structures, which can serve as a fundamental technique for developing high-performance room-temperature gas sensors.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":null,"pages":null},"PeriodicalIF":8.2,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141597877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multipressure Sampling for Improving the Performance of MOF-based Electronic Noses. 多压力取样技术用于提高基于 MOF 的电子鼻的性能。
IF 8.2 1区 化学
ACS Sensors Pub Date : 2024-07-12 DOI: 10.1021/acssensors.4c00199
Brian A Day, Nicolas I Ahualli, Christopher E Wilmer
{"title":"Multipressure Sampling for Improving the Performance of MOF-based Electronic Noses.","authors":"Brian A Day, Nicolas I Ahualli, Christopher E Wilmer","doi":"10.1021/acssensors.4c00199","DOIUrl":"https://doi.org/10.1021/acssensors.4c00199","url":null,"abstract":"<p><p>Metal-organic frameworks (MOFs) are a promising class of porous materials for the design of gas sensing arrays, which are often called electronic noses. Due to their chemical and structural tunability, MOFs are a highly diverse class of materials that align well with the similarly diverse class of volatile organic compounds (VOCs) of interest in many gas detection applications. In principle, by choosing the right combination of cross-sensitive MOFs, layered on appropriate signal transducers, one can design an array that yields detailed information about the composition of a complex gas mixture. However, despite the vast number of MOFs from which one can choose, gas sensing arrays that rely too heavily on distinct chemistries can be impractical from the cost and complexity perspective. On the other hand, it is difficult for small arrays to have the desired selectivity and sensitivity for challenging sensing applications, such as detecting weakly adsorbing gases with weak signals, or conversely, strongly adsorbing gases that readily saturate MOF pores. In this work, we employed gas adsorption simulations to explore the use of a variable pressure sensing array as a means of improving both sensitivity and selectivity as well as increasing the information content provided by each array. We studied nine different MOFs (HKUST-1, IRMOF-1, MgMOF-74, MOF-177, MOF-801, NU-100, NU-125, UiO-66, and ZIF-8) and four different gas mixtures, each containing nitrogen, oxygen, carbon dioxide, and exactly one of the hydrogen, methane, hydrogen sulfide, or benzene. We found that by lowering the pressure, we can limit the saturation of MOFs, and by raising the pressure, we can concentrate weakly adsorbing gases, in both cases, improving gas detection with the resulting arrays. In many cases, changing the system pressure yielded a better improvement in performance (as measured by the Kullback-Liebler divergence of gas composition probability distributions) than including additional MOFs. We thus demonstrated and quantified how sensing at multiple pressures can increase information content and cross-sensitivity in MOF-based arrays while limiting the number of unique materials needed in the device.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":null,"pages":null},"PeriodicalIF":8.2,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141597875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
From Organ-on-a-Chip to Human-on-a-Chip: A Review of Research Progress and Latest Applications. 从芯片上的器官到芯片上的人:研究进展和最新应用综述。
IF 8.2 1区 化学
ACS Sensors Pub Date : 2024-07-11 DOI: 10.1021/acssensors.4c00004
Yisha Huang, Tong Liu, Qi Huang, Yuxi Wang
{"title":"From Organ-on-a-Chip to Human-on-a-Chip: A Review of Research Progress and Latest Applications.","authors":"Yisha Huang, Tong Liu, Qi Huang, Yuxi Wang","doi":"10.1021/acssensors.4c00004","DOIUrl":"https://doi.org/10.1021/acssensors.4c00004","url":null,"abstract":"<p><p>Organ-on-a-Chip (OOC) technology, which emulates the physiological environment and functionality of human organs on a microfluidic chip, is undergoing significant technological advancements. Despite its rapid evolution, this technology is also facing notable challenges, such as the lack of vascularization, the development of multiorgan-on-a-chip systems, and the replication of the human body on a single chip. The progress of microfluidic technology has played a crucial role in steering OOC toward mimicking the human microenvironment, including vascularization, microenvironment replication, and the development of multiorgan microphysiological systems. Additionally, advancements in detection, analysis, and organoid imaging technologies have enhanced the functionality and efficiency of Organs-on-Chips (OOCs). In particular, the integration of artificial intelligence has revolutionized organoid imaging, significantly enhancing high-throughput drug screening. Consequently, this review covers the research progress of OOC toward Human-on-a-chip, the integration of sensors in OOCs, and the latest applications of organoid imaging technologies in the biomedical field.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":null,"pages":null},"PeriodicalIF":8.2,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pt Loading of Phosphorus-Doped Carbon Nanotube Aerogels in Fuel Cell-Type Gas Sensors for Ultrasensitive H2 Detection. 燃料电池型气体传感器中掺磷碳纳米管气凝胶的铂负载,用于超灵敏 H2 检测。
IF 8.2 1区 化学
ACS Sensors Pub Date : 2024-07-10 DOI: 10.1021/acssensors.4c00948
Lingchu Huang, Huaiyuan Sun, Weijia Li, Jianyu Zhang, Sitong Feng, Qi Lu, Tong Wang, Xishuang Liang, Fangmeng Liu, Fengmin Liu, Geyu Lu
{"title":"Pt Loading of Phosphorus-Doped Carbon Nanotube Aerogels in Fuel Cell-Type Gas Sensors for Ultrasensitive H<sub>2</sub> Detection.","authors":"Lingchu Huang, Huaiyuan Sun, Weijia Li, Jianyu Zhang, Sitong Feng, Qi Lu, Tong Wang, Xishuang Liang, Fangmeng Liu, Fengmin Liu, Geyu Lu","doi":"10.1021/acssensors.4c00948","DOIUrl":"https://doi.org/10.1021/acssensors.4c00948","url":null,"abstract":"<p><p>A phosphorus-doped carbon nanotube (CNT) aerogel as the support material was loaded with Pt nanoparticles in fuel cell-type gas sensors for ultrasensitive H<sub>2</sub> detection. The high surface area of the CNT scaffold is favorable to providing abundant active sites, and the high electrical conductivity facilitates the transport of carriers generated by electrochemical reactions. In addition, the CNT aerogel was doped with phosphorus (P) to further enhance the conductivity and electrochemical catalytic activity. As a result, the fuel cell-type gas sensor using the Pt/CNT aerogel doped with the optimal P content as the sensing material shows considerable performance for H<sub>2</sub> detection at room temperature. The sensor exhibits an ultrahigh response of -921.9 μA to 15,000 ppm of H<sub>2</sub>. The sensitivity is -0.063 μA/ppm, which is 21 times higher than that of the conventional Pt/CF counterpart. The sensor also exhibits excellent repeatability and humidity resistance, as well as fast response/recovery; the response/recovery times are 31 and 4 s to 3000 ppm of H<sub>2</sub>, respectively. The modulation of the structure and catalytic properties of the support material is responsible for the improvement of the sensor performance, thus providing a feasible solution for optimizing the performance of fuel cell-type gas sensors.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":null,"pages":null},"PeriodicalIF":8.2,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141561923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ambipolar Heterojunction Sensors: Another Way to Detect Polluting Gases Ambipolar 异质结传感器:检测污染气体的另一种方法
IF 8.9 1区 化学
ACS Sensors Pub Date : 2024-07-10 DOI: 10.1021/acssensors.4c00900
Sujithkumar Ganesh Moorthy, Seydou Ouedraogo, Marcel Bouvet
{"title":"Ambipolar Heterojunction Sensors: Another Way to Detect Polluting Gases","authors":"Sujithkumar Ganesh Moorthy, Seydou Ouedraogo, Marcel Bouvet","doi":"10.1021/acssensors.4c00900","DOIUrl":"https://doi.org/10.1021/acssensors.4c00900","url":null,"abstract":"Gas sensors based on ambipolar materials offer significant advantages in reducing the size of the analytical system and enhancing its efficiency. Here, bilayer heterojunction devices are constructed using different octafluorinated phthalocyanine complexes, with Zn and Co as metal centers, combined with a lutetium bisphthalocyanine complex (LuPc<sub>2</sub>). Stable p-type behavior is observed for the ZnF<sub>8</sub>Pc/LuPc<sub>2</sub> device under both electron-donating (NH<sub>3</sub>) and -oxidizing (NO<sub>2</sub> and O<sub>3</sub>) gaseous species, while the CoF<sub>8</sub>Pc/LuPc<sub>2</sub> device exhibits n-type behavior under reducing gases and p-type behavior under oxidizing gases. The nature of majority of the charge carriers of Co-based devices varies depending on the nature of target gases, displaying an ambipolar behavior. Both heterojunction devices demonstrate stable and observable response toward all three toxic gases in the sub-ppm range. Remarkably, the Co-based device is highly sensitive toward ammonia with a limit of detection (LOD) of 200 ppb, whereas the Zn-based device demonstrates exceptional sensitivity toward oxidizing gases, with excellent LOD values of 4.9 and 0.75 ppb toward NO<sub>2</sub> and O<sub>3</sub>, respectively, which makes it one of the most effective organic heterojunction sensors reported so far for oxidizing gases.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":null,"pages":null},"PeriodicalIF":8.9,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141566369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Parylene Double-Layer Coated Screen-Printed Carbon Electrode for Label-Free and Reagentless Capacitive Aptasensing of Gliadin. 聚对二甲苯双层涂层丝网印刷碳电极,用于无标签、无试剂的麸质蛋白电容感应。
IF 8.2 1区 化学
ACS Sensors Pub Date : 2024-07-09 DOI: 10.1021/acssensors.4c00875
Chun-Ning Tsai, Chin-Yun Lee, Hsien-Yeh Chen, Bo-Chuan Hsieh
{"title":"Parylene Double-Layer Coated Screen-Printed Carbon Electrode for Label-Free and Reagentless Capacitive Aptasensing of Gliadin.","authors":"Chun-Ning Tsai, Chin-Yun Lee, Hsien-Yeh Chen, Bo-Chuan Hsieh","doi":"10.1021/acssensors.4c00875","DOIUrl":"https://doi.org/10.1021/acssensors.4c00875","url":null,"abstract":"<p><p>Celiac patients are required to strictly adhere to a gluten-free diet because even trace amounts of gluten can damage their small intestine and leading to serious complications. Despite increased awareness, gluten can still be present in products due to cross-contamination or hidden ingredients, making regular monitoring essential. With the goal of guaranteeing food safety for consuming labeled gluten-free products, a capacitive aptasensor was constructed to target gliadin, the main allergic gluten protein for celiac disease. The success of capacitive aptasensing was primarily realized by coating a Parylene double-layer (1000 nm Parylene C at the bottom with 400 nm Parylene AM on top) on the electrode surface to ensure both high insulation quality and abundant reactive amino functionalities. Under the optimal concentration of aptamer (5 μM) used for immobilization, a strong linear relationship exists between the amount of gliadin (0.01-1.0 mg/mL) and the corresponding Δ<i>C</i> response (total capacitance decrease during a 20 min monitoring period after sample introduction), with an <i>R</i><sup>2</sup> of 0.9843. The detection limit is 0.007 mg/mL (S/<i>N</i> > 5), equivalent to 0.014 mg/mL (14 ppm) of gluten content. Spike recovery tests identified this system is free from interferences in corn and cassava flour matrices. The analytical results of 24 commercial wheat flour samples correlated well with a gliadin ELISA assay (<i>R</i><sup>2</sup> = 0.9754). The proposed label-free and reagentless capacitive aptasensor offers advantages of simplicity, cost-effectiveness, ease of production, and speediness, making it a promising tool for verifying products labeled as gluten-free (gluten content <20 ppm).</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":null,"pages":null},"PeriodicalIF":8.2,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141561922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiplexing Imaging of Closely Located Single-Nucleotide Mutations in Single Cells via Encoded in situ PCR. 通过编码原位 PCR 对单细胞中位置较近的单核苷酸突变进行多重成像
IF 8.2 1区 化学
ACS Sensors Pub Date : 2024-07-09 DOI: 10.1021/acssensors.4c00378
Yao Ren, Kerui Liu, Hao Yang, Yong Zhang, Sha Deng, Jijuan Cao, Xuhan Xia, Ruijie Deng
{"title":"Multiplexing Imaging of Closely Located Single-Nucleotide Mutations in Single Cells via Encoded <i>in situ</i> PCR.","authors":"Yao Ren, Kerui Liu, Hao Yang, Yong Zhang, Sha Deng, Jijuan Cao, Xuhan Xia, Ruijie Deng","doi":"10.1021/acssensors.4c00378","DOIUrl":"https://doi.org/10.1021/acssensors.4c00378","url":null,"abstract":"<p><p>Mutation accumulation in RNAs results in closely located single-nucleotide mutations (SNMs), which is highly associated with the drug resistance of pathogens. Imaging of SNMs in single cells has significance for understanding the heterogeneity of RNAs that are related to drug resistance, but the direct \"see\" closely located SNMs remains challenging. Herein, we designed an encoded ligation-mediated <i>in situ</i> polymerase chain reaction method (termed enPCR), which enabled the visualization of multiple closely located SNMs in bacterial RNAs. Unlike conventional ligation-based probes that can only discriminate a single SNM, this method can simultaneously image different SNMs at closely located sites with single-cell resolution using modular anchoring probes and encoded PCR primers. We tested the capacity of the method to detect closely located SNMs related to quinolone resistance in the <i>gyrA</i> gene of <i>Salmonella enterica</i> (<i>S. enterica</i>), and found that the simultaneous detection of the closely located SNMs can more precisely indicate the resistance of the <i>S. enterica</i> to quinolone compared to the detection of one SNM. The multiplexing imaging assay for SNMs can serve to reveal the relationship between complex cellular genotypes and phenotypes.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":null,"pages":null},"PeriodicalIF":8.2,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141561921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
FRETting about CRISPR-Cas Assays: Dual-Channel Reporting Lowers Detection Limits and Times-to-Result. 关于 CRISPR-Cas 检测的 FRETting:双通道报告降低了检测限和结果时间。
IF 8.2 1区 化学
ACS Sensors Pub Date : 2024-07-08 DOI: 10.1021/acssensors.4c00652
Jake M Lesinski, Nathan K Khosla, Carolina Paganini, Bo Verberckmoes, Heleen Vermandere, Andrew J deMello, Daniel A Richards
{"title":"FRETting about CRISPR-Cas Assays: Dual-Channel Reporting Lowers Detection Limits and Times-to-Result.","authors":"Jake M Lesinski, Nathan K Khosla, Carolina Paganini, Bo Verberckmoes, Heleen Vermandere, Andrew J deMello, Daniel A Richards","doi":"10.1021/acssensors.4c00652","DOIUrl":"https://doi.org/10.1021/acssensors.4c00652","url":null,"abstract":"<p><p><u>C</u>lustered <u>R</u>egularly <u>I</u>nterspaced <u>S</u>hort <u>P</u>alindromic <u>R</u>epeats-<u>C</u>RISPR-<u>A</u>ssociated <u>P</u>rotein (CRISPR-Cas) systems have evolved several mechanisms to specifically target foreign DNA. These properties have made them attractive as biosensors. The primary drawback associated with contemporary CRISPR-Cas biosensors is their weak signaling capacity, which is typically compensated for by coupling the CRISPR-Cas systems to nucleic acid amplification. An alternative strategy to improve signaling capacity is to engineer the reporter, i.e., design new signal-generating substrates for Cas proteins. Unfortunately, due to their reliance on custom synthesis, most of these engineered reporter substrates are inaccessible to many researchers. Herein, we investigate a substrate based on a fluorescein (FAM)-tetramethylrhodamine (TAMRA) Förster resonant energy-transfer (FRET) pair that functions as a seamless \"drop-in\" replacement for existing reporters, without the need to change any other aspect of a CRISPR-Cas12a-based assay. The reporter is readily available and employs FRET to produce two signals upon cleavage by Cas12a. The use of both signals in a ratiometric manner provides for improved assay performance and a decreased time-to-result for several CRISPR-Cas12a assays when compared to a traditional FAM-Black Hole Quencher (BHQ) quench-based reporter. We comprehensively characterize this reporter to better understand the reasons for the improved signaling capacity and benchmark it against the current standard CRISPR-Cas reporter. Finally, to showcase the real-world utility of the reporter, we employ it in a <u>R</u>ecombinase <u>P</u>olymerase <u>A</u>mplification (RPA)-CRISPR-Cas12a <u>D</u>NA <u>E</u>ndonuclease-<u>T</u>arget<u>e</u>d <u>C</u>RISPR <u>T</u>rans <u>R</u>eporter (DETECTR) assay to detect <i>Human papillomavirus</i> in patient-derived samples.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":null,"pages":null},"PeriodicalIF":8.2,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141557364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Shining Light on Osteoarthritis: Spatially Offset Raman Spectroscopy as a Window into Cartilage Health. 照亮骨关节炎:空间偏移拉曼光谱是了解软骨健康的窗口
IF 8.2 1区 化学
ACS Sensors Pub Date : 2024-07-08 DOI: 10.1021/acssensors.4c01242
Piyush Raj, Lintong Wu, Craig Almeida, Lauren Conway, Swati Tanwar, Jill Middendorf, Ishan Barman
{"title":"Shining Light on Osteoarthritis: Spatially Offset Raman Spectroscopy as a Window into Cartilage Health.","authors":"Piyush Raj, Lintong Wu, Craig Almeida, Lauren Conway, Swati Tanwar, Jill Middendorf, Ishan Barman","doi":"10.1021/acssensors.4c01242","DOIUrl":"https://doi.org/10.1021/acssensors.4c01242","url":null,"abstract":"<p><p>Articular cartilage is a complex tissue, and early detection of osteoarthritis (OA) is crucial for effective treatment. However, current imaging modalities lack molecular specificity and primarily detect late-stage changes. In this study, we propose the use of spatially offset Raman spectroscopy (SORS) for noninvasive, depth-dependent, and molecular-specific diagnostics of articular cartilage. We demonstrate the potential of SORS to penetrate deep layers of cartilage, providing a comprehensive understanding of disease progression. Our SORS measurements were characterized and validated through mechanical and histological techniques, revealing strong correlations between spectroscopic measurements and both Young's modulus and depth of cartilage damage. By longitudinally monitoring enzymatically degraded condyles, we further developed a depth-dependent damage-tracking method. Our analysis revealed distinct components related to sample depth and glycosaminoglycan (GAG) changes, offering a comprehensive picture of cartilage health. Collectively, these findings highlight the potential of SORS as a valuable tool for enhancing OA management and improving patient outcomes.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":null,"pages":null},"PeriodicalIF":8.2,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141557365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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