IF 8.9 1区化学

ACS Sensors Pub Date : 2025-10-13 DOI: 10.1021/acssensors.5c01878

Sung Wook Choi,Dong Hyeong Kim,Soo-Hwan Jeong,Ju Hyeon Kim,Jae Bem You

{"title":"Trap-Based Microfluidic Device with Retrivable Droplet for the Analysis of Pollutants from Slurry Solutions.","authors":"Sung Wook Choi,Dong Hyeong Kim,Soo-Hwan Jeong,Ju Hyeon Kim,Jae Bem You","doi":"10.1021/acssensors.5c01878","DOIUrl":"https://doi.org/10.1021/acssensors.5c01878","url":null,"abstract":"Liquid-liquid microextraction (LLME) is a cost-effective sample pretreatment method for the analysis of trace analytes in various samples, such as water, blood, urine, and others. In LLME, the high surface area-to-volume ratio of the extractant enables fast accumulation of analytes from the sample into the extractant. The analyte-loaded extractant can easily be analyzed by using common analytical instruments such as liquid or gas chromatography, mass spectrometry, and others. However, a critical drawback of LLME is that when the sample contains solid fragments, microextraction must be preceded by a removal step, which can result in the loss of the analyte and compromise the detection. In this work, we demonstrate microextraction of analytes from a slurry solution without the need for an additional pretreatment step using a trap-based microfluidic device with a retrievable extractant. The extractant, 1-Octanol, is loaded into a microchamber that is in contact with a microchannel that continuously introduces the sample solution. As the solution flows in the microchannel, the intimate contact of the solution with the extractant enables the fast extraction of analytes. Using Nile red (NR) as a model analyte, we demonstrate the influence of the analyte concentration and the flow rate of the sample solution on the extraction. As a proof-of-concept, we show successful extraction of trace amounts of perfluorooctanoic acid (PFOA) from water. More importantly, the device enables one-step extraction of target analytes, e.g., carbamazepine (CBZ), from a concentrated sand suspension. The extracted CBZ is easily analyzed by using high-performance liquid chromatography, eliminating the need to remove the solid content prior to extraction. The approach shown in this work can potentially be applied to various fields, such as drug detection, environmental pollution monitoring, or food safety for pesticide residue analysis.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"73 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surfactant-Mediated Droplet Spreading Analysis on Liquid Crystal-Infused Porous Surfaces: A Label-free Biosensor for Sensitive Lipopolysaccharide Detection. 表面活性剂介导的液滴在液晶注入多孔表面上的扩散分析：一种用于敏感脂多糖检测的无标记生物传感器。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-10-13 DOI: 10.1021/acssensors.5c01864

Binglu Zhao,Won Tae Hong,Lili Wang,Dong Zhang,Jung Kyu Kim,Qiongzheng Hu,Woo-Seok Choe

{"title":"Surfactant-Mediated Droplet Spreading Analysis on Liquid Crystal-Infused Porous Surfaces: A Label-free Biosensor for Sensitive Lipopolysaccharide Detection.","authors":"Binglu Zhao,Won Tae Hong,Lili Wang,Dong Zhang,Jung Kyu Kim,Qiongzheng Hu,Woo-Seok Choe","doi":"10.1021/acssensors.5c01864","DOIUrl":"https://doi.org/10.1021/acssensors.5c01864","url":null,"abstract":"Lipopolysaccharide (LPS), a potent endotoxin from Gram-negative bacteria, poses significant clinical and industrial challenges, necessitating sensitive and reliable detection methods. In clinical diagnostics, early LPS detection is essential for timely intervention in sepsis, where endotoxemia drives systemic inflammation. In the biopharmaceutical industry, stringent LPS monitoring ensures the safety of therapeutic proteins, as even trace levels can trigger severe immune responses. We present a novel biosensing platform for LPS detection based on the spreading behavior of surfactant-laden droplets on liquid crystal-infused porous surfaces (LCIPS). Aqueous droplets containing cetyltrimethylammonium bromide (CTAB) exhibit large spreading areas due to enhanced surface wetting on LCIPS. The addition of an LPS-specific aptamer reduces droplet spreading by forming a CTAB-aptamer complex. Upon LPS introduction, preferential aptamer-LPS binding competitively disrupts this complex, restoring droplet spreading, with the final droplet spreading area (DSA) proportional to LPS concentration. Systematic optimization of CTAB (50 μM) and aptamer (0.1 μM) concentrations enables detection sensitivity down to 0.051 EU/mL, with performance validated against a commercial endotoxin testing system. The platform exhibits high specificity for intact LPS and maintains robust functionality across complex sample matrices, including tap water, drinking water, and injectable solutions. This simple, label-free, and cost-effective biosensor holds strong potential for sepsis diagnostics, pharmaceutical quality control, and environmental monitoring.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"103 3 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water-Responsive Xerogel-Based Flood Alarm Device Utilizing Smartphone Electrogenerated Chemiluminescence Imaging 基于智能手机电致化学发光成像的水响应干凝胶洪水报警装置

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-10-13 DOI: 10.1021/acssensors.5c02165

Yiran Ma, Wenhui Cai, Chengxiao Zhang, Xiaolin Yang, Honglan Qi

{"title":"Water-Responsive Xerogel-Based Flood Alarm Device Utilizing Smartphone Electrogenerated Chemiluminescence Imaging","authors":"Yiran Ma, Wenhui Cai, Chengxiao Zhang, Xiaolin Yang, Honglan Qi","doi":"10.1021/acssensors.5c02165","DOIUrl":"https://doi.org/10.1021/acssensors.5c02165","url":null,"abstract":"The global incidence of flash floods has increased, leading to fatalities and significant economic losses. Hence, the development of cost-effective, timely, and efficient flood early warning systems holds significant economic and social value. In this work, a flash flood alarm device was fabricated that employed water-responsive xerogel as a core material and utilized smartphone electrogenerated chemiluminescence (ECL) imaging as the detecting technique. The water-responsive ECL xerogel was derived from the drying process of a conductive ECL hydrogel, where the conductive ECL hydrogel material was prepared by doping both Ru(bpy)<sub>3</sub>Cl<sub>2</sub> luminophore and conductive indium tin oxide nanoparticles into the flexible hydrophilic polymer matrix via the crosslinking of poly(vinyl alcohol) with borax and sucrose. Intriguingly, the ECL xerogel exhibits a rapid response to water and displays a significantly enhanced ECL behavior in the presence of water. Moreover, one flood alarm device was fabricated by the incorporation of one serrate FTO electrode as the anode, five pieces of the ECL xerogel, a strip-type FTO electrode as the cathode, a battery group as the power supply, and a smartphone as the detection device. This flood alarm device demonstrates effective performance in water level warning functionalities even in dim or nocturnal conditions with cost-effectiveness, good sensitivity, and resolution, which are associated with the greatest risk of flooding. This work provides a flood alarm device based on an ECL xerogel utilizing smartphones as detectors, which is promising for the early warning of flood.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"19 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Contrast Aptamer-Based Merocyanine Displacement Assays for Sensitive Small Molecule Detection 基于高对比度适配体的Merocyanine置换法用于敏感小分子检测

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-10-13 DOI: 10.1021/acssensors.5c02194

Thinh Nguyen, Obtin Alkhamis, Adara Bacon, Konstantin V. Bukhryakov, Nelson R. Vinueza, Yi Xiao

{"title":"High-Contrast Aptamer-Based Merocyanine Displacement Assays for Sensitive Small Molecule Detection","authors":"Thinh Nguyen, Obtin Alkhamis, Adara Bacon, Konstantin V. Bukhryakov, Nelson R. Vinueza, Yi Xiao","doi":"10.1021/acssensors.5c02194","DOIUrl":"https://doi.org/10.1021/acssensors.5c02194","url":null,"abstract":"Aptamer-based colorimetric dye-displacement assays offer a promising means for on-site detection due to their single-step mix-and-read procedure and rapid naked-eye readout. Here, analyte binding to noncovalent aptamer-dye complexes triggers the displacement of the dye into solution, resulting in the formation of aggregates with divergent optical absorbance properties from the monomeric aptamer-bound dye. However, these assays suffer from low sensitivity, poor color contrast, and small absorbance wavelength shifts between the dye monomers and aggregates, as well as the large spectral overlaps between these two forms. Here, we introduce the solvatochromic coumarin-based merocyanine dye X-19539-107 as a novel indicator that enables the highly sensitive detection of small-molecule targets in dye-displacement assays. In aqueous solution, the dye forms H-aggregates that absorb at 445 nm, whereas aptamer-bound dye monomers absorb at 610–625 nm─a difference of up to 180 nm, with minimal spectral overlap. We utilize X-19539-107 in dye-displacement assays with diverse aptamers to detect various small-molecule analytes with unprecedentedly high target-induced dye displacement efficiency and greater sensitivity than conventional cyanine indicators and excellent color contrast. We also demonstrate cocaine detection at toxicologically relevant levels in saliva, highlighting the real-world utility of these assays. We conclude that our merocyanine dye offers a powerful alternative to cyanine dyes for colorimetric aptamer-based sensing.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"210 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Fibrosis-Targeting T1 MRI Contrast Agent Synthesized via Photooxidative Self-Desulfurization of Porphyrin Thiourea. 卟啉硫脲光氧化自脱硫合成的纤维化靶向T1 MRI造影剂。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-10-13 DOI: 10.1021/acssensors.5c02443

Kyle D W Vollett,Anlan Hong,Wanda W Janaeska,Daryn R Browne,Hai-Ling Margaret Cheng

{"title":"A Fibrosis-Targeting T1 MRI Contrast Agent Synthesized via Photooxidative Self-Desulfurization of Porphyrin Thiourea.","authors":"Kyle D W Vollett,Anlan Hong,Wanda W Janaeska,Daryn R Browne,Hai-Ling Margaret Cheng","doi":"10.1021/acssensors.5c02443","DOIUrl":"https://doi.org/10.1021/acssensors.5c02443","url":null,"abstract":"Fibrosis is a silent disease that becomes untreatable and incurable in late stages, ultimately leading to organ failure. Early intervention can slow or even reverse progression, but current noninvasive tools like magnetic resonance imaging (MRI) and ultrasound are nonspecific and detect only advanced scar tissue. We present a fibrosis-targeting, nongadolinium MRI contrast agent for sensitive and specific in vivo imaging of fibrosis. Unlike conventional methods that infer scar content via stiffness or empty dead tissue space, our agent directly binds the excess collagen substrate of the scar tissue. To synthesize the agent, we conjugated an \"MRI active\" manganese porphyrin to a fibrosis-targeting free-base porphyrin via the first demonstration of porphyrin photooxidative self-desulfurization of aryl thiourea linkages to stable urea or guanidine linkages. The compound exhibited high affinity for acid-soluble collagen in a new scar and significantly reduced T1 relaxation time in collagen gels. In a mouse model of diffuse myocardial fibrosis, the fibrosis-targeting agent highlighted scar tissue that was undetected by conventional late-gadolinium MRI.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"53 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inherently Flexible Sn1-xSbxO2 Solid Solution Nanofibers with Light-Driven Plasmon for Wearable Room-Temperature Gas Sensors. 可穿戴室温气体传感器用固有柔性Sn1-xSbxO2固溶体纳米纤维驱动等离子体激元。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-10-12 DOI: 10.1021/acssensors.5c02345

Rui Tang,Xiaowei Li,Yu Liu,Wanying Cheng,Haipeng Dong,Mengjie Guan,Haoqian Luo,Tong Liu,Xinghua Li,Changlu Shao,Yichun Liu

{"title":"Inherently Flexible Sn1-xSbxO2 Solid Solution Nanofibers with Light-Driven Plasmon for Wearable Room-Temperature Gas Sensors.","authors":"Rui Tang,Xiaowei Li,Yu Liu,Wanying Cheng,Haipeng Dong,Mengjie Guan,Haoqian Luo,Tong Liu,Xinghua Li,Changlu Shao,Yichun Liu","doi":"10.1021/acssensors.5c02345","DOIUrl":"https://doi.org/10.1021/acssensors.5c02345","url":null,"abstract":"The rapid advancement of the Internet of Things technology has driven a significant demand for wearable and portable gas sensors. However, the inherent brittleness and rigidity of inorganic resistive metal oxide semiconductors (MOSs) limit their flexibility in wearable sensor applications. Herein, for the first time, we present a novel approach to address these limitations by developing inherently flexible gas sensors using electrospun Sn1-xSbxO2 solid solution nanofibers. The self-supported nanofiber membranes exhibit remarkable inherent flexibility, which can be attributed to their ultrafine grain size and hybrid amorphous-crystalline structure that effectively mitigates the formation of macroscopic cracks within the Sn1-xSbxO2 nanofibers. Furthermore, these Sn1-xSbxO2 solid solution nanofibers demonstrate pronounced surface plasmon resonance absorption in the visible-light spectrum, enabling visible-light-driven room-temperature detection of nitrogen dioxide (NO2) at parts-per-billion (ppb) levels, which enhances their applicability for wearable devices. Additionally, the unique nanofiber network structure significantly improves air permeability, thereby facilitating gas sensing reactions while enhancing user comfort. These findings pave the way for the development of wearable real-time gas monitoring technologies, addressing critical challenges within the gas sensing field.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"18 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial-Temporal Multiomics Profiling of Living Single Cells on Chip for Cancer Therapy. 芯片上活单细胞的时空多组学分析用于癌症治疗。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-10-12 DOI: 10.1021/acssensors.5c01502

Kunru Yu,Shengjie Chen,Rong Zhu

{"title":"Spatial-Temporal Multiomics Profiling of Living Single Cells on Chip for Cancer Therapy.","authors":"Kunru Yu,Shengjie Chen,Rong Zhu","doi":"10.1021/acssensors.5c01502","DOIUrl":"https://doi.org/10.1021/acssensors.5c01502","url":null,"abstract":"Single-cell molecular profiling has emerged as a cutting-edge technology in biological research, playing a pivotal role in elucidating fundamental life processes and cellular heterogeneity. However, traditional methods generally provide end point measurements and require cell disruption, posing challenges for long-term monitoring of living cell events, e.g., cancer therapy. Tumor Treating Fields (TTFields), a noninvasive therapeutic modality, exert inhibitory effects on tumor growth and invasion by applying low-intensity intermediate-frequency alternating electric fields to tumor regions, whose molecular mechanism on cancer physiology remains poorly understood. In this study, we propose a multifunctional microchip capable of continuous in situ monitoring of molecular signatures in living single cells. The platform integrates spatial cell positioning, single-cellular multimolecule tracking with surface-enhanced Raman scattering (SERS), and TTFields treatment functionalities on a microchip. Using multiplexed analysis of liver cancer cells treated with TTFields and chemotherapy drugs, we implement spatial-temporal multiomics profiling for cancer therapy. Temporal tracking of critical molecular events associated with glycolysis, genetic stability, and membrane integrity reveals significant differences between the groups with and without TTFields treatment. Causal network analysis of molecular profiles uncovers the underlying mechanisms through which TTFields modulate oncogenic pathways. Our work demonstrates the effectiveness of the proposed microchip platform for real-time monitoring of living cell molecular dynamics, offering a powerful tool for precision cancer therapy.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"22 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "A Nanomaterial-Independent Biosensor Based on Gallium Arsenide High-Electron-Mobility Transistors for Rapid and Ultra-Sensitive Pathogen Detection". 修正“基于砷化镓高电子迁移率晶体管的非纳米材料生物传感器，用于快速和超灵敏的病原体检测”。

IF 9.1 1区化学

ACS Sensors Pub Date : 2025-10-11 DOI: 10.1021/acssensors.5c03002

Ying Zheng, Chunyang Zhang, Ying Zhang, Kun Zhou, Pengwei Tan, Youjiang Liu, Guotao Duan, Hengfei Li, Chilai Chen, Cunlan Guo, Xiaodong Li, Chen Chen, Ashaq Ali, Yang Zhang, Xian-En Zhang, Dong Men

引用次数: 0

Deep Learning–Aided Noninvasive Monitoring of Skin Tissue Temperature Distribution and Blood Perfusion Rate Based on Fractal Conformal Sensors 基于分形保形传感器的深度学习辅助无创皮肤组织温度分布和血流灌注率监测

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-10-10 DOI: 10.1021/acssensors.5c02023

Yuxin Ouyang, Yanhui Feng, Yongzheng Han, Lin Qiu

{"title":"Deep Learning–Aided Noninvasive Monitoring of Skin Tissue Temperature Distribution and Blood Perfusion Rate Based on Fractal Conformal Sensors","authors":"Yuxin Ouyang, Yanhui Feng, Yongzheng Han, Lin Qiu","doi":"10.1021/acssensors.5c02023","DOIUrl":"https://doi.org/10.1021/acssensors.5c02023","url":null,"abstract":"Skin thermophysical properties are key for health assessment with real-time monitoring enabling early detection of skin-related issues. A polydimethylsiloxane-encapsulated Peano fractal conformal sensor is fabricated by flexible printed circuit technology to accurately measure skin tissue temperature distribution and dermal blood perfusion rate while maintaining full conformity. The second-harmonic method enables precise thermophysical property extraction without requiring high-precision lock-in amplifiers. A circular heat model and multitask learning convolutional neural network (MTLCNN) facilitate rapid thermophysical property prediction, while a thermal impedance network captures temperature distribution during measurements. The sensor provides stable measurements of thermal conductivity and diffusivity with 6.6% uncertainty at a ∼57.3° bending angle. The MTLCNN model achieves a combined correlation coefficient of 0.9054, demonstrating a superior regression performance. Interactions among thermophysical properties, perfusion rate, and temperature distribution support thermal balance in the human body. This approach offers valuable insights for improving noninvasive health monitoring and diagnostic.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"101 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145255191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RAPPID-M: A Mix-and-Measure Bioluminescent Sandwich Immunoassay Based on Generic Antibody-Binding Protein M. RAPPID-M：一种基于通用抗体结合蛋白M的混合测量生物发光三明治免疫分析法。

IF 8.9 1区化学

ACS Sensors Pub Date : 2025-10-10 DOI: 10.1021/acssensors.5c02450

Anna Swietlikowska,Eva van Aalen,Max Bossink,Laura van Weesep,Maarten Merkx

{"title":"RAPPID-M: A Mix-and-Measure Bioluminescent Sandwich Immunoassay Based on Generic Antibody-Binding Protein M.","authors":"Anna Swietlikowska,Eva van Aalen,Max Bossink,Laura van Weesep,Maarten Merkx","doi":"10.1021/acssensors.5c02450","DOIUrl":"https://doi.org/10.1021/acssensors.5c02450","url":null,"abstract":"Homogeneous immunoassays that allow direct in-sample detection of biomarkers provide an attractive alternative to traditional heterogeneous immunoassays that require multiple washing and incubation steps. We previously reported the development of RAPPID, a bioluminescent sensor platform that uses split luciferase complementation to enable sandwich immunoassays directly in solution. Although RAPPID provides many benefits over traditional heterogeneous immunoassays, it requires protein G-mediated photoconjugation of split luciferase fragments to the Fc part of IgG monoclonal antibodies. Here, we report a new generation of RAPPID sensors (RAPPID-M) that do not rely on photoconjugation of protein G, but instead use a generic antibody-binding domain derived from protein M. The use of protein M substantially expands the application of RAPPID to include protein G-incompatible but analytically important mouse IgG1 antibodies, as well as scFv and Fab antibody fragments. Moreover, binding of protein M is found to be essentially irreversible, abolishing the need for photo-cross-linking. RAPPID-M thus improves upon the original RAPPID platform by the mix-and-measure-type assembly of antibody-split-luciferase conjugates and its compatibility with a broader scope of antibody types without compromising and even improving the analytical performance.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"9 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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