表面活性剂介导的液滴在液晶注入多孔表面上的扩散分析:一种用于敏感脂多糖检测的无标记生物传感器。

IF 9.1 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Binglu Zhao,Won Tae Hong,Lili Wang,Dong Zhang,Jung Kyu Kim,Qiongzheng Hu,Woo-Seok Choe
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引用次数: 0

摘要

脂多糖(LPS)是一种来自革兰氏阴性菌的强效内毒素,给临床和工业带来了重大挑战,需要敏感可靠的检测方法。在临床诊断中,早期检测LPS对于脓毒症的及时干预至关重要,因为脓毒症的内毒素血症会引发全身性炎症。在生物制药行业,严格的LPS监测确保了治疗蛋白的安全性,因为即使是微量水平也会引发严重的免疫反应。我们提出了一种基于表面活性剂负载液滴在液晶注入多孔表面(LCIPS)上扩散行为的新型LPS检测生物传感平台。含有十六烷基三甲基溴化铵(CTAB)的水滴由于在LCIPS上增强了表面润湿而表现出较大的扩散面积。添加lps特异性适配体通过形成ctab -适配体复合物减少液滴扩散。引入LPS后,优先的适体-LPS结合竞争性地破坏该复合物,恢复液滴扩散,最终液滴扩散面积(DSA)与LPS浓度成正比。CTAB (50 μM)和适体(0.1 μM)浓度的系统优化使检测灵敏度降至0.051 EU/mL,并通过商用内毒素检测系统进行了性能验证。该平台对完整的LPS具有高特异性,并在复杂的样品基质(包括自来水、饮用水和注射溶液)中保持强大的功能。这种简单,无标签,具有成本效益的生物传感器在败血症诊断,药物质量控制和环境监测方面具有强大的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Surfactant-Mediated Droplet Spreading Analysis on Liquid Crystal-Infused Porous Surfaces: A Label-free Biosensor for Sensitive Lipopolysaccharide Detection.
Lipopolysaccharide (LPS), a potent endotoxin from Gram-negative bacteria, poses significant clinical and industrial challenges, necessitating sensitive and reliable detection methods. In clinical diagnostics, early LPS detection is essential for timely intervention in sepsis, where endotoxemia drives systemic inflammation. In the biopharmaceutical industry, stringent LPS monitoring ensures the safety of therapeutic proteins, as even trace levels can trigger severe immune responses. We present a novel biosensing platform for LPS detection based on the spreading behavior of surfactant-laden droplets on liquid crystal-infused porous surfaces (LCIPS). Aqueous droplets containing cetyltrimethylammonium bromide (CTAB) exhibit large spreading areas due to enhanced surface wetting on LCIPS. The addition of an LPS-specific aptamer reduces droplet spreading by forming a CTAB-aptamer complex. Upon LPS introduction, preferential aptamer-LPS binding competitively disrupts this complex, restoring droplet spreading, with the final droplet spreading area (DSA) proportional to LPS concentration. Systematic optimization of CTAB (50 μM) and aptamer (0.1 μM) concentrations enables detection sensitivity down to 0.051 EU/mL, with performance validated against a commercial endotoxin testing system. The platform exhibits high specificity for intact LPS and maintains robust functionality across complex sample matrices, including tap water, drinking water, and injectable solutions. This simple, label-free, and cost-effective biosensor holds strong potential for sepsis diagnostics, pharmaceutical quality control, and environmental monitoring.
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来源期刊
ACS Sensors
ACS Sensors Chemical Engineering-Bioengineering
CiteScore
14.50
自引率
3.40%
发文量
372
期刊介绍: ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.
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