Biomaterials Science最新文献

Correction: Synthesis of poly-tetrahydropyrimidine antibacterial polymers and research of their basic properties.

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-20 DOI: 10.1039/d5bm90016f

Taimin Xu, Wenlong Li, Rong Zhang, Shuaibing Guo, Bing Yu, Hailin Cong, Youqing Shen

引用次数: 0

Correction: Photothermal-responsive Prussian blue nanocages loaded with thrombin for tumor starvation therapy and photothermal therapy.

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-20 DOI: 10.1039/d5bm90018b

Yang Liu, Bingjie Yue, Ranran Wang, Hailin Cong, Hao Hu, Bing Yu, Youqing Shen

引用次数: 0

Correction: D-A-D organic fluorescent probes for NIR-II fluorescence imaging and efficient photothermal therapy of breast cancer.

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-20 DOI: 10.1039/d5bm90017d

Jie Gao, Lin Yuan, Yu Min, Bing Yu, Hailin Cong, Youqing Shen

引用次数: 0

Influence of lung extracellular matrix from non-IPF and IPF donors on primary human lung fibroblast biology.

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-19 DOI: 10.1039/d4bm00906a

Mohammadhossein Dabaghi, Ryan Singer, Alex Noble, Aidee Veronica Arizpe Tafoya, David A González-Martínez, Tamaghna Gupta, Cécile Formosa-Dague, Ivan O Rosas, Martin R Kolb, Yaron Shargall, Jose M Moran-Mirabal, Jeremy A Hirota

{"title":"Influence of lung extracellular matrix from non-IPF and IPF donors on primary human lung fibroblast biology.","authors":"Mohammadhossein Dabaghi, Ryan Singer, Alex Noble, Aidee Veronica Arizpe Tafoya, David A González-Martínez, Tamaghna Gupta, Cécile Formosa-Dague, Ivan O Rosas, Martin R Kolb, Yaron Shargall, Jose M Moran-Mirabal, Jeremy A Hirota","doi":"10.1039/d4bm00906a","DOIUrl":"https://doi.org/10.1039/d4bm00906a","url":null,"abstract":"Fibrosis, a pathological hallmark of various chronic diseases, involves the excessive accumulation of extracellular matrix (ECM) components leading to tissue scarring and functional impairment. Understanding how cells interact with the ECM in fibrotic diseases such as idiopathic pulmonary fibrosis (IPF), is crucial for developing effective therapeutic strategies. This study explores the effects of decellularized extracellular matrix (dECM) coatings derived from non-IPF and IPF donor lung tissue samples on the behavior of primary human lung fibroblasts (HLFs). Utilizing a substrate coating method that preserves the diversity of in situ ECM, we studied both the concentration-dependent effects and the intrinsic biochemical cues of ECM on cell morphology, protein expression, mechanobiology biomarkers, and gene expression. Morphological analysis revealed that HLFs displayed altered spreading, shape, and nuclear characteristics in response to dECM coatings relative to control plastic, indicating a response to the physical and biochemical cues. Protein expression studies showed an upregulation of α-smooth muscle actin (α-SMA) in cells interacting with both non-IPF and IPF dECM coatings, that was more prominent at IPF dECM-coated surface. In addition, YAP localization, a marker of mechanotransduction, was also dysregulated on dECM coatings, reflecting changes in mechanical signaling pathways. Gene expression profiles were differentially regulated by the different dECM coatings. The developed dECM coating strategy in this work facilitates the integration of tissue-specific biochemical cues onto standard cell culture platforms, which is ideal for high-throughput screening. Importantly, it minimizes the requirement for human tissue samples, especially when compared to more sample-intensive 3D models like dECM-based hydrogels.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of ionizable groups in star polymer nanoparticles on NLRP3 inflammasome activation.

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-18 DOI: 10.1039/d4bm01349b

Mehak Malhotra, Sarmishta Thodur, Ashish Kulkarni

{"title":"Impact of ionizable groups in star polymer nanoparticles on NLRP3 inflammasome activation.","authors":"Mehak Malhotra, Sarmishta Thodur, Ashish Kulkarni","doi":"10.1039/d4bm01349b","DOIUrl":"https://doi.org/10.1039/d4bm01349b","url":null,"abstract":"The advent of cancer nanovaccines (N.V.s) has transformed immunotherapy by using nanoparticles as biologic delivery vehicles or vaccine adjuvants. However, challenges remain due to nanoparticle-immune cell interactions. Investigating nanoparticle (N.P.) physicochemical effects on the innate immune system is crucial for safe biomaterials design. The NLRP3 inflammasome, a key innate immunity component, is implicated in many inflammatory disorders. Various nanoparticle-associated molecular patterns (NAMPs) trigger NLRP3 activation, but the combined effect of these NAMPs in a single N.P. platform is not well understood. Star polymer nanocarriers were chosen to study the impact of combined hydrophobic and ionizable groups on NLRP3 activation. Star polymers offer stable self-assembly, high drug/gene encapsulation, and enhanced cellular internalization. We designed 4-arm star random copolymers with constant hydrophobic moiety and varied ionizable groups to evaluate their NLRP3 activation in macrophages. The study revealed differences in cytokine release and cell death linked to ionizable groups, providing insights for selecting safe, immunomodulatory biomaterials.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microgel-based modular 3D in vitro microfluidic cell culture platforms.

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-18 DOI: 10.1039/d4bm00891j

Manleen Kaur, Mayuri Dutta, Soutik Betal, Neetu Singh

{"title":"Microgel-based modular 3D in vitro microfluidic cell culture platforms.","authors":"Manleen Kaur, Mayuri Dutta, Soutik Betal, Neetu Singh","doi":"10.1039/d4bm00891j","DOIUrl":"https://doi.org/10.1039/d4bm00891j","url":null,"abstract":"The combination of 3D in vitro cell culture and microfluidic technology has emerged as a powerful approach in biomedical engineering. It offers a more physiologically relevant model compared to traditional 2D cell cultures by allowing the assembly of micro-sized cellular structures, known as microgels. These microgels can be prepared and fabricated to mimic the in vivo characteristics of an ECM. We report here an economical and feasible microfluidic 3D in vitro culture platform that offers real-time monitoring of cellular proliferation by encapsulating pH-sensing carbon dots (CDs) with cells in the microgels. These CDs were shown to effectively evaluate proliferation within cell-encapsulated microgels in comparison with the traditional Alamar blue assay. The biggest advantage of this platform is its ability to co-culture different cell types, achieved by encapsulating the cells within individual microgels, spatially separating them while maintaining close proximity. In this modular system, each microgel acts as a unit of a specific cell type, allowing easy retrieval of cells and control over cell densities. We established the efficacy of this concept by co-culturing Huh-7 and NIH-3T3 cells within different microgel combinations, under both static and dynamic flow conditions. The heterotypic interactions were explored by assessing the functionality using albumin assay and CYP3A4 gene expression studies, along with performing drug toxicity assays. The functionality studies confirmed results from existing literature studies by showing an improved hepatic function in the presence of NIH-3T3, even in the dynamic state. This platform can be expanded to include multiple cell types, creating a complex tissue-like effect without requiring spatial patterning techniques.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Branched silver-iron oxide nanoparticles enabling highly effective targeted and localised drug-free thrombolysis.

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-17 DOI: 10.1039/d4bm01089b

Karla X Vazquez-Prada, Shehzahdi S Moonshi, Yuao Wu, Karlheinz Peter, Xiaowei Wang, Zhi Ping Xu, Hang Thu Ta

引用次数: 0

Titanium implants trigger extra-periodontal T cell-mediated immunity. 钛种植体会引发牙周外 T 细胞介导的免疫。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-17 DOI: 10.1039/d4bm00246f

Li-Tzu Wang, Sin-Ei Juang, Chi-Lun Lan, Hsuan-Hao Chang, Ai-Chia He, Wei-An Chen, Yu-Wen Huang, Thomas E Van Dyke, Yi-Wen Chen, Kevin Sheng-Kai Ma

{"title":"Titanium implants trigger extra-periodontal T cell-mediated immunity.","authors":"Li-Tzu Wang, Sin-Ei Juang, Chi-Lun Lan, Hsuan-Hao Chang, Ai-Chia He, Wei-An Chen, Yu-Wen Huang, Thomas E Van Dyke, Yi-Wen Chen, Kevin Sheng-Kai Ma","doi":"10.1039/d4bm00246f","DOIUrl":"https://doi.org/10.1039/d4bm00246f","url":null,"abstract":"Peri-implant inflammation and periodontitis share a common etiology rooted in periodontopathic bacterial invasion, with periodontitis notably linked to systemic inflammatory comorbidities involving T cells. However, the intricate processes within the peri-implant microenvironment and systemic repercussions of implants, particularly related to implant materials, remain inadequately understood. We aim to elucidate the impact of contact with titanium materials, widely employed in dental implants for their high biocompatibility and excellent corrosion resistance, on diverse T cell subpopulations. This study adopts a comprehensive approach, encompassing (1) transcriptomic profiling of peri-implant epithelium in a rat model, (2) examination of phenotypic and functional changes in T cell immunity in human blood cells cultured on titanium discs, and (3) in vivo validation of T cells in implanted mice. Transcriptomic evidence and functional in vitro results revealed that exposure to titanium materials promoted T cell activation and differentiation towards inflammatory subsets, and escalated the secretion of corresponding cytokines. In vivo results showed that most of the gingiva-extracted T cells were activated in both healthy and implanted mice, the latter exhibiting significant lymphadenitis. High-dimensional flow cytometric findings in the in vivo lymphadenitis model indicated titanium-induced T cell immunity, involving preferential activation of Th1, Th17, and Tc1 cells over Tregs in adjacent lymph nodes within three days after implant placement. These findings highlight the pivotal role of T cells in the initiation of peri-implant inflammation, emphasizing the need to understand extra-periodontal inflammatory complications associated with implant surgeries. Our study provides a foundation for future therapeutic strategies targeting T cell responses to enhance the success and longevity of dental implant treatments.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curcumin and vitamin D3 release from calcium phosphate enhances bone regeneration.

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-17 DOI: 10.1039/d4bm01188k

Yongdeok Jo, Priya Kushram, Susmita Bose

引用次数: 0

Enhanced combination therapy through tumor microenvironment-activated cellular uptake and ROS-sensitive drug release using a dual-sensitive nanogel.

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-02-17 DOI: 10.1039/d4bm01377h

Jianming Yuan, Qinfeng Chen, Mingxiang Zuo, Xiaoxia Li, ChiYi Ou, Qinghua Chen, Dongsheng Yu, Haowen Li, Chenhui Hao, Jing Yang, Shuang Liu, Du Cheng

{"title":"Enhanced combination therapy through tumor microenvironment-activated cellular uptake and ROS-sensitive drug release using a dual-sensitive nanogel.","authors":"Jianming Yuan, Qinfeng Chen, Mingxiang Zuo, Xiaoxia Li, ChiYi Ou, Qinghua Chen, Dongsheng Yu, Haowen Li, Chenhui Hao, Jing Yang, Shuang Liu, Du Cheng","doi":"10.1039/d4bm01377h","DOIUrl":"https://doi.org/10.1039/d4bm01377h","url":null,"abstract":"Although the co-delivery of chemotherapeutic and photodynamic agents has been studied for years, developing a simple and efficient nanoplatform for high co-delivery efficiency remains a challenge for clinical applications. In this study, we prepared a reactive oxygen species (ROS) and pH dual-sensitive nanogel for the co-encapsulation of doxorubicin (DOX) and indocyanine green (ICG)-conjugated bovine serum albumin (BSA) via a simple inverse miniemulsion polymerization process. This was followed by modification with pegylated cell-penetrating peptides (CPPs) containing citraconic anhydride (CDM) linkers, which are sensitive to weakly acidic microenvironments (pH 6.5). Pegylation endowed the nanogel with extended blood circulation, while the de-shielding of polyethylene glycol (PEG) exposed the CPPs, significantly enhancing cellular uptake. Upon near-infrared (NIR) irradiation, ROS generated by ICG not only killed tumor cells but also triggered the release of DOX through nanogel disintegration. Serial experiments verified the nanogel's high co-delivery efficiency, tumor tissue matrix microenvironment-triggered cellular uptake, controlled drug release, and synergistic antitumor effects. Therefore, this dual-sensitive nanogel, prepared via inverse miniemulsion polymerization, offers a facile approach to improving co-delivery efficiency for combination therapy.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0