Biomaterials Science最新文献

Correction: Bioactivity of cerium dioxide nanoparticles as a function of size and surface features. 更正：二氧化铈纳米粒子的生物活性与尺寸和表面特征的关系。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-11-18 DOI: 10.1039/d4bm90076f

Veronika Sarnatskaya, Yuliia Shlapa, Denis Kolesnik, Olexandra Lykhova, Dmytro Klymchuk, Serhii Solopan, Svitlana Lyubchyk, Iuliia Golovynska, Junle Qu, Yurii Stepanov, Anatolii Belous

引用次数: 0

Adhesive silk fibroin/magnesium composite films and their application for removable wound dressing. 粘性丝纤维素/镁复合膜及其在可移动伤口敷料中的应用。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-11-18 DOI: 10.1039/d4bm01411a

Qiaolin Chen, Kang Wu, Jinrong Yao, Zhengzhong Shao, Xin Chen

{"title":"Adhesive silk fibroin/magnesium composite films and their application for removable wound dressing.","authors":"Qiaolin Chen, Kang Wu, Jinrong Yao, Zhengzhong Shao, Xin Chen","doi":"10.1039/d4bm01411a","DOIUrl":"https://doi.org/10.1039/d4bm01411a","url":null,"abstract":"Silk fibroin is a naturally abundant biomaterial renowned for its excellent biocompatibility and biodegradability, making it a promising candidate for biomedical applications like wound dressings. However, traditional silk fibroin materials often lack sufficient mechanical strength, adhesion, and the ability to modulate inflammation and oxidative stress-factors crucial for effective wound healing. To address these limitations, regenerated silk fibroin/magnesium ion [RSF/Mg(II)] composite films were developed by incorporating Mg(II) ions into RSF solutions. These films were characterized using Raman spectroscopy, mechanical testing, and biocompatibility assessments, and their wound-healing efficacy was evaluated in a mouse skin defect model. The RSF/Mg(II) composite films exhibited superior adhesion, higher transparency, and enhanced mechanical flexibility compared to pristine RSF films. They also demonstrated anti-inflammatory and antioxidative properties, effectively reducing cell apoptosis and reactive oxygen species levels in vitro. In vivo, the RSF/Mg Mg(II) composite films significantly accelerated wound healing in mice, improving epidermal thickness, collagen deposition, and promoting blood vessel formation. This study highlights the potential of RSF/Mg(II) composite films as advanced wound dressings with improved biocompatibility and biological activity, offering valuable insights for the development of Mg(II) ion-based biomaterials in wound healing and tissue regeneration applications.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cholesterol- and ssDNA-binding fusion protein-mediated DNA tethering on the plasma membrane. 胆固醇和 ssDNA 结合融合蛋白介导的 DNA 在质膜上的系留。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-11-18 DOI: 10.1039/d4bm01127a

Kei Nishida, Minon Ishizuka, Eiry Kobatake, Masayasu Mie

{"title":"Cholesterol- and ssDNA-binding fusion protein-mediated DNA tethering on the plasma membrane.","authors":"Kei Nishida, Minon Ishizuka, Eiry Kobatake, Masayasu Mie","doi":"10.1039/d4bm01127a","DOIUrl":"https://doi.org/10.1039/d4bm01127a","url":null,"abstract":"DNA modification of the plasma membrane is an excellent approach for controlling membrane-protein interactions, modulating cell-cell/cell-biomolecule interactions, and extending the biosensing field. The hydrophobic insertion of DNA conjugated with hydrophobic anchoring molecules is utilized for tethering DNA on the cell membrane. In this study, we developed an alternative approach to tether DNA on the plasma membrane based on ssDNA- and cholesterol-binding proteins. We designed a fusion protein (Rep-ALOD4) composed of domain 4 of anthrolysin O (ALOD4), which binds to cholesterol in the plasma membrane, and a replication initiator protein derived from porcine circovirus type 2 (Rep), which forms covalent bonds with single-stranded DNA (ssDNA) with a Rep recognition sequence. Rep-ALOD4 conjugates ssDNA to Rep and binds to the plasma membrane via cholesterol, thus tethering ssDNA to the cells. Quartz crystal microbalance measurements showed that membrane cholesterol binding of Rep-ALOD4 to the lipid bilayer containing cholesterol was accelerated above 20% (w/w) cholesterol in the lipid bilayer. Rep-ALOD4 was conjugated to fluorescein-labeled ssDNA (S-FITC-Rep-ALOD4) and used to treat human cervical tumor HeLa cells. The green signal assigned to S-FITC-Rep-ALOD4 was detected along HeLa cells, whereas diminished by cholesterol removal with methyl β-cyclodextrins. Moreover, ssDNA-conjugated Rep-ALOD4 tethered ssDNA-conjugated functional proteins on the HeLa cell plasma membrane via complementary base pairing. Collectively, Rep-ALOD4 has the potential as an ssDNA-tethering material via plasma membrane cholesterol to extend cell surface engineering.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A glucose responsive multifunctional hydrogel with antibacterial properties and real-time monitoring for diabetic wound treatment. 一种具有抗菌特性和实时监控功能的葡萄糖反应多功能水凝胶，用于糖尿病伤口治疗。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-11-14 DOI: 10.1039/d4bm01097c

Zhifei Yang, Jiaxu Zhang, Chen Wang, Fangzheng Yu, Wen Yu, Zheng Zhao

{"title":"A glucose responsive multifunctional hydrogel with antibacterial properties and real-time monitoring for diabetic wound treatment.","authors":"Zhifei Yang, Jiaxu Zhang, Chen Wang, Fangzheng Yu, Wen Yu, Zheng Zhao","doi":"10.1039/d4bm01097c","DOIUrl":"https://doi.org/10.1039/d4bm01097c","url":null,"abstract":"The healing of complex diabetic wounds with a hyperglycemic microenvironment and bacterial infection is considered an important clinical issue. In this study, glucose oxidase (GOx) and gold nanoclusters (AuNCs) were encapsulated in quaternary carboxymethyl chitosan (QCMCS)/sodium alginate oxide (OSA) hydrogels and were immersed in tannic acid (TA) solution to achieve antioxidant, antibacterial, pro-angiogenesis, pro-collagen deposition and real-time monitoring functions. In vitro studies showed that TA-QCMCS/OSA@GOx@AuNC hydrogels had inhibition rates of 98.99% and 99.99% against S. aureus and E. coli, respectively, and the survival rate of mouse fibroblasts (L929) was over 95%. In vivo studies showed that TA-QCMCS/OSA@GOx@AuNC hydrogels were 97.28% effective in healing diabetic wounds. In addition, image signals from TA-QCMCS/OSA@GOx@AuNC hydrogels can be collected in real time to accurately obtain glucose concentration values of diabetic wounds and reflect the healing status of diabetic wounds in a timely manner. The results showed that TA-QCMCS/OSA@GOx@AuNC hydrogels provide a novel idea for real-time monitoring of diabetic wound treatment.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supramolecular multiplexes from collagen mimetic peptide-PNA(GGG)₃ conjugates and C-rich DNA: pH-induced reversible switching from triplex-duplex to triplex-i-motif. 胶原蛋白模拟肽-PNA(GGG)3 共轭物和富含 C 的 DNA 的超分子复合物：pH 值诱导的从三重双工到三重单工的可逆转换。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-11-14 DOI: 10.1039/d4bm00955j

Shahaji H More, Marc Schmutz, Loïc Jierry, Krishna N Ganesh

{"title":"Supramolecular multiplexes from collagen mimetic peptide-PNA(GGG)3 conjugates and C-rich DNA: pH-induced reversible switching from triplex-duplex to triplex-i-motif.","authors":"Shahaji H More, Marc Schmutz, Loïc Jierry, Krishna N Ganesh","doi":"10.1039/d4bm00955j","DOIUrl":"https://doi.org/10.1039/d4bm00955j","url":null,"abstract":"Peptides are well known for forming nanoparticles, while DNA duplexes, triplexes and tetraplexes create rigid nanostructures. Accordingly, the covalent conjugation of peptides to DNA/RNA produces hybrid self-assembling features and may lead to interesting nano-assemblies distinct from those of their individual components. Herein, we report the preparation of a collagen mimetic peptide incorporating lysine in its backbone, with alkylamino side chains radially conjugated with G-rich PNA [collagen-(PNA-GGG)3]. In the presence of complementary C-rich DNA (dCCCTTTCCC) at neutral pH, the collagen mimetic triplexes were interconnected by PNA-GGG : DNA-CCC duplexes, leading to the formation of larger assemblies of nanostructures. Upon decreasing the pH to 4.5, the dissociation of the triplex-duplex assembly released the protonated C-rich DNA, which immediately folded into an i-motif. With an increase in the pH to 7.2 (neutral), the i-motif unfolded into linear DNA, which reformed the PNA-GGG : DNA-CCC duplex interconnecting the collagen triplexes. The pH-induced switching of the assembly and disassembly was reversible over a few cycles. The hybrid collagen-(PNAGGG)3 : DNA-C3T3C3 triplex-duplex and the individual components of the assembly including the i-motif were characterized by UV and CD melting, fluorescence, TEM and gel electrophoresis. The pH-induced reversible switching was established by the changes in the CD and fluorescence properties. Peptide-DNA conjugates have wide applications in both biology and materials science, ranging from therapeutics and drug delivery to diagnostics and molecular switches. Thus, the prototype ensemble of the triplex peptide-PNA conjugate and its duplex with DNA described herein has potential for elaboration into rationally designed systems by varying the PNA/DNA sequences to trap functional ligands/drugs for release in pH-controlled environments.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hijacking plant skeletons for biomedical applications: from regenerative medicine and drug delivery to biosensing. 将植物骨骼用于生物医学应用：从再生医学、药物输送到生物传感。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-11-13 DOI: 10.1039/d4bm00982g

Elham Asadian, Samin Abbaszadeh, Fatemeh Ghorbani-Bidkorpeh, Saman Rezaei, Bo Xiao, Hélder A Santos, Mohammad-Ali Shahbazi

{"title":"Hijacking plant skeletons for biomedical applications: from regenerative medicine and drug delivery to biosensing.","authors":"Elham Asadian, Samin Abbaszadeh, Fatemeh Ghorbani-Bidkorpeh, Saman Rezaei, Bo Xiao, Hélder A Santos, Mohammad-Ali Shahbazi","doi":"10.1039/d4bm00982g","DOIUrl":"https://doi.org/10.1039/d4bm00982g","url":null,"abstract":"The field of biomedical engineering continually seeks innovative technologies to address complex healthcare challenges, ranging from tissue regeneration to drug delivery and biosensing. Plant skeletons offer promising opportunities for these applications due to their unique hierarchical structures, desirable porosity, inherent biocompatibility, and adjustable mechanical properties. This review comprehensively discusses chemical principles underlying the utilization of plant-based scaffolds in biomedical engineering. Highlighting their structural integrity, tunable properties, and possibility of chemical modification, the review explores diverse preparation strategies to tailor plant skeleton properties for bone, neural, cardiovascular, skeletal muscle, and tendon tissue engineering. Such applications stem from the cellulosic three-dimensional structure of different parts of plants, which can mimic the complexity of native tissues and extracellular matrices, providing an ideal environment for cell adhesion, proliferation, and differentiation. We also discuss the application of plant skeletons as carriers for drug delivery due to their structural diversity and versatility in encapsulating and releasing therapeutic agents with controlled kinetics. Furthermore, we present the emerging role played by plant-derived materials in biosensor development for diagnostic and monitoring purposes. Challenges and future directions in the field are also discussed, offering insights into the opportunities for future translation of sustainable plant-based technologies to address critical healthcare needs.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering considerations in the design of tissue specific bioink for 3D bioprinting applications. 三维生物打印应用中组织特异性生物墨水设计的工程考虑因素。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-11-13 DOI: 10.1039/d4bm01192a

Shivi Tripathi, Madhusmita Dash, Ruchira Chakraborty, Harri Junaedi Lukman, Prasoon Kumar, Shabir Hassan, Hassan Mehboob, Harpreet Singh, Himansu Sekhar Nanda

{"title":"Engineering considerations in the design of tissue specific bioink for 3D bioprinting applications.","authors":"Shivi Tripathi, Madhusmita Dash, Ruchira Chakraborty, Harri Junaedi Lukman, Prasoon Kumar, Shabir Hassan, Hassan Mehboob, Harpreet Singh, Himansu Sekhar Nanda","doi":"10.1039/d4bm01192a","DOIUrl":"https://doi.org/10.1039/d4bm01192a","url":null,"abstract":"Over eight million surgical procedures are conducted annually in the United Stats to address organ failure or tissue losses. In response to this pressing need, recent medical advancements have significantly improved patient outcomes, primarily through innovative reconstructive surgeries utilizing tissue grafting techniques. Despite tremendous efforts, repairing damaged tissues remains a major clinical challenge for bioengineers and clinicians. 3D bioprinting is an additive manufacturing technique that holds significant promise for creating intricately detailed constructs of tissues, thereby bridging the gap between engineered and actual tissue constructs. In contrast to non-biological printing, 3D bioprinting introduces added intricacies, including considerations for material selection, cell types, growth, and differentiation factors. However, technical challenges arise, particularly concerning the delicate nature of living cells in bioink for tissue construction and limited knowledge about the cell fate processes in such a complex biomechanical environment. A bioink must have appropriate viscoelastic and rheological properties to mimic the native tissue microenvironment and attain desired biomechanical properties. Hence, the properties of bioink play a vital role in the success of 3D bioprinted substitutes. This review comprehensively delves into the scientific aspects of tissue-centric or tissue-specific bioinks and sheds light on the current challenges of the translation of bioinks and bioprinting.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-assembled biomimetic fibrils from collagen and chitosan for performance-enhancing hemostatic dressing. 胶原蛋白和壳聚糖共组装生物仿生纤维，用于增强止血敷料的性能。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-11-12 DOI: 10.1039/d4bm01211a

Xingling Zeng, Zhaohui Sun, Lidan Chen, Xiaoxia Zhang, Xin Guo, Guoying Li

{"title":"Co-assembled biomimetic fibrils from collagen and chitosan for performance-enhancing hemostatic dressing.","authors":"Xingling Zeng, Zhaohui Sun, Lidan Chen, Xiaoxia Zhang, Xin Guo, Guoying Li","doi":"10.1039/d4bm01211a","DOIUrl":"https://doi.org/10.1039/d4bm01211a","url":null,"abstract":"The development of safe and efficient hemostatic materials is medically important to prevent death due to trauma bleeding. Exploiting the synergistic effect between the D-periodic functional domain of collagen fibrils on platelet activation and cationic chitosan on erythrocyte aggregation is expected to develop performance-enhanced hemostatic materials. In this study, we prepared collagen fibrils and chitosan composite hemostatic materials by modulating the self-assembled bionic fibrillation of collagen with different degrees of deacetylation (DD, 50%, 70% and 85%) of chitosan. The findings indicated that chitosan promoted collagen self-assembly, with all the collagen fibrils demonstrating a typical D-periodical structure similar to that of the native collagen. Furthermore, the composite demonstrated enhanced structural integrity and procoagulant capacity along with good biocompatibility. Notably, the fibrillar composites with 70% DD of chitosan exhibited optimal mechanical properties, procoagulant activity, and adhesion of erythrocytes and platelets. Compared to pure collagen fibrils and the commercial hemostatic agent Celox™, the collagen/chitosan fibrillar composite treatment significantly accelerated hemostasis in the rat tail amputation model and liver injury model. This research offers new insights into the development of hemostatic materials and indicates that collagen-chitosan composites hold promising potential for clinical applications.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoinitiator-free light-mediated crosslinking of dynamic polymer and pristine protein networks. 无光引发剂的光介导动态聚合物和原始蛋白质网络交联。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-11-12 DOI: 10.1039/d4bm00849a

Riccardo Rizzo, Dylan M Barber, Jackson K Wilt, Alexander J Ainscough, Jennifer A Lewis

{"title":"Photoinitiator-free light-mediated crosslinking of dynamic polymer and pristine protein networks.","authors":"Riccardo Rizzo, Dylan M Barber, Jackson K Wilt, Alexander J Ainscough, Jennifer A Lewis","doi":"10.1039/d4bm00849a","DOIUrl":"10.1039/d4bm00849a","url":null,"abstract":"Light-based patterning of synthetic and biological hydrogels enables precise spatial and temporal control over the formation of chemical bonds. However, photoinitiators are typically used to generate free radicals, which are detrimental to human cells. Here, we report a photoinitiator- and radical-free method based on ortho-nitrobenzyl alcohol (oNBA) photolysis, which gives rise to highly reactive nitroso and benzaldehyde groups. Synthetic hydrogel and pristine protein networks can rapidly form in the presence of these photo-generated reactive species. Thiol -oNBA bonds yield dynamic hydrogel networks (DHNs) via N-semimercaptal linkages that exhibit thixotropy, stress relaxation, and on-demand reversible gel-to-liquid transitions, while amine-oNBA bonds can be exploited to crosslink pristine proteins, such as gelatin and fibrinogen, by targeting their primary amines. Since this approach does not require incorporation of photoreactive moieties along the backbone, the resulting crosslinked proteins are well suited for bioadhesives. Our photoinitiator-free platform provides a versatile approach for rapidly creating synthetic and biological hydrogels for applications ranging from tissue engineering to biomedical devices.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11562384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid neurostimulation at the micron scale with an optically controlled thermal-capture technique. 利用光学控制热捕捉技术在微米尺度上进行快速神经刺激。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-11-12 DOI: 10.1039/d4bm01114g

Alexey M Romshin, Nikolay A Aseyev, Olga S Idzhilova, Alena A Koryagina, Vadim E Zeeb, Igor I Vlasov, Pavel M Balaban

{"title":"Rapid neurostimulation at the micron scale with an optically controlled thermal-capture technique.","authors":"Alexey M Romshin, Nikolay A Aseyev, Olga S Idzhilova, Alena A Koryagina, Vadim E Zeeb, Igor I Vlasov, Pavel M Balaban","doi":"10.1039/d4bm01114g","DOIUrl":"https://doi.org/10.1039/d4bm01114g","url":null,"abstract":"Precise control of cellular temperature at the microscale is crucial for developing novel neurostimulation techniques. Here, the effect of local heat on the electrophysiological properties of primary neuronal cultures and HEK293 cells at the subcellular level using a cutting-edge micrometer-scale thermal probe, the diamond heater-thermometer (DHT), is studied. A new mode of local heat action on a living cell, thermal-capture mode (TCM), is discovered using the DHT probe. In TCM, the application of a 50 °C temperature step induces a great increase in cellular response, allowing the cell to be thermally captured and depolarized by up to 20 mV. This thermal effect is attributed to local phase changes in the phospholipid membrane, enabling precise and reproducible modulation of cell activity. The TCM is shown to open up new opportunities for thermal cell stimulation. DHT reliably triggers action potentials (APs) in neurons at rates up to 30 Hz, demonstrating the ability to control cell excitability with millisecond and sub-millisecond resolution. AP shape is modulated by local heat as well. The ability to precisely control the AP shape and rate via thermal-capture mode opens new avenues for non-invasive, localized neurostimulation techniques, particularly in controlling neuron excitability.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0