Biomaterials Science最新文献

{"title":"Correction: Construction of a sustained-release hydrogel using gallic acid and lysozyme with antimicrobial properties for wound treatment","authors":"Wei Gong, Hai-bo Huang, Xin-chuang Wang, Wan-ying He, Yi-yang Hou and Jiang-ning Hu","doi":"10.1039/D5BM90007G","DOIUrl":"10.1039/D5BM90007G","url":null,"abstract":"<p >Correction for ‘Construction of a sustained-release hydrogel using gallic acid and lysozyme with antimicrobial properties for wound treatment’ by Wei Gong <em>et al.</em>, <em>Biomater. Sci.</em>, 2022, <strong>10</strong>, 6836–6849, https://doi.org/10.1039/D2BM00658H.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 4","pages":" 1101-1101"},"PeriodicalIF":5.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm90007g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of a micropatterned shape-memory polymer patch with l-DOPA for tendon regeneration†","authors":"Yucheol Son, Min Suk Lee, Dong Jun Hwang, Sun Hong Lee, Albert S. Lee, Seung Sang Hwang, Dong Hoon Choi, Chris Hyunchul Jo and Hee Seok Yang","doi":"10.1039/D4BM00298A","DOIUrl":"10.1039/D4BM00298A","url":null,"abstract":"<p >A scaffold design for tendon regeneration has been proposed, which mimics the microstructural features of tendons and provides appropriate mechanical properties. We synthesized a temperature-triggered shape-memory polymer (SMP) using the ring-opening polymerization of polycaprolactone (PCL) with polyethylene glycol (PEG) as a macroinitiator. We fabricated a micropatterned patch using SMP <em>via</em> capillary force lithography, which mimicked a native tendon, for providing physical cues and guiding effects. The SMP patches (the SMP-flat patch is referred to as SMP-F, and the SMP-patterned patch is referred to as SMP-P) were surface-modified with 3,4-dihydroxy-<small>L</small>-phenylalanine (<small>L</small>-DOPA, referred to as D) for improving cell adhesion. We hypothesized that SMP patches could be applied in minimally invasive surgery and the micropatterned structure would improve tendon regeneration by providing geometrical cues. The SMP patches exhibited excellent shape-memory properties, mechanical performance, and biocompatibility <em>in vitro</em> and <em>in vivo</em>. Especially, SMP-DP demonstrated enhanced cell behaviors <em>in vitro</em>, including cell orientation, elongation, migration, and tenogenic differentiation potential. The <em>in vivo</em> data showed notable biomechanical functionality and histological morphometric findings in various analyses of SMP-DP in the ruptured Achilles tendon model.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 5","pages":" 1243-1260"},"PeriodicalIF":5.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioorthogonal reaction-mediated photosensitizer–peptide conjugate anchoring on cell membranes for enhanced photodynamic therapy†","authors":"Buwei Hu, Chenlin Ji, Zhuohang Zhou, Xuehan Xu, Luyi Wang, Tingting Cao, Jianjun Cheng and Rui Sun","doi":"10.1039/D4BM01602E","DOIUrl":"10.1039/D4BM01602E","url":null,"abstract":"<p >Photodynamic therapy (PDT), utilizing a photosensitizer (PS) to induce tumor cell death, is an effective modality for cancer treatment. PS–peptide conjugates have recently demonstrated remarkable antitumor potential in preclinical trials. However, the limited cell membrane binding affinity and rapid systemic clearance have hindered their transition to clinical applications. To address these challenges, we investigated whether <em>in vivo</em> covalent chemistry could enhance tumor accumulation and potentiate antitumor efficacy. Specifically, we synthesized a PS–peptide conjugate termed P-DBCO-Ce6, with chlorin e6 (Ce6) and dibenzocyclooctyne (DBCO) conjugated to a negatively charged short peptide. By employing metabolic glycoengineering and bioorthogonal reactions, P-DBCO-Ce6 achieves covalent bonding to the cell membrane, enabling prolonged retention of the PS on the cell surface and the <em>in situ</em> generation of reactive oxygen species (ROS) on cell membranes to kill tumor cells. <em>In vivo</em> studies demonstrated a 3.3-fold increase in tumor accumulation of the PS through bioorthogonal reactions compared to the control group, confirming that click chemistry can effectively enhance PS tumor accumulation. This approach allows for the effective elimination of tumors with a single treatment. The improved efficiency of this strategy provides new insights into the design of PDT systems for potential clinical applications.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 5","pages":" 1233-1242"},"PeriodicalIF":5.8,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinted ferret mesenchymal stem cell-laden cartilage grafts for laryngotracheal reconstruction in a ferret surgical model†","authors":"Alexandra McMillan, Matthew R. Hoffman, Yan Xu, Zongliang Wu, Emma Thayer, Adreann Peel, Allan Guymon, Sohit Kanotra and Aliasger K. Salem","doi":"10.1039/D4BM01251H","DOIUrl":"10.1039/D4BM01251H","url":null,"abstract":"<p >Chondrogenic differentiation of mesenchymal stem cells (MSCs) within a three-dimensional (3D) environment can be guided to form cartilage-like tissue <em>in vitro</em> to generate cartilage grafts for implantation. 3D bioprinted, MSC-populated cartilage grafts have the potential to replace autologous cartilage in reconstructive airway surgery. Here, bone marrow-derived ferret MSCs (fMSCs) capable of directed musculoskeletal differentiation were generated for the first time. A multi-material, 3D bioprinted fMSC-laden scaffold was then engineered that was capable of <em>in vitro</em> cartilage regeneration, as evidenced by glycosaminoglycan (GAG) production and collagen II immunohistochemical staining. <em>In vivo</em> implantation of these 3D bioprinted scaffolds in a ferret model of laryngotracheal reconstruction (LTR) demonstrated healing of the defect site, epithelial mucosalization of the inner lumen, and expansion of the airway volume. While the implanted scaffold allowed for reconstruction of the created airway defect, minimal chondrocytes were identified at the implant site. Nevertheless, we have established the ferret as a biomedical research model for airway reconstruction and, although further evaluation is warranted, the generation of fMSCs provides an opportunity for realizing the potential for 3D bioprinted regenerative stem cell platforms in the ferret.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 5","pages":" 1304-1322"},"PeriodicalIF":5.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of tildipirosin-loaded solid lipid nanoparticles for the treatment of intracellular Staphylococcus aureus infections†","authors":"Zhixin Lei, Xiaoli Cai, Juan Wan, Ze Li and Taolei Sun","doi":"10.1039/D4BM01428F","DOIUrl":"10.1039/D4BM01428F","url":null,"abstract":"<p >To enhance the antibacterial efficacy of tildipirosin against <em>Staphylococcus aureus</em> (S.A.) infections, optimized solid lipid nanoparticles loaded with tildipirosin (SLN-TD) were developed, using docosanoic acid (DA), octadecanoic acid (OA), hexadecanoic acid (HA), and tetradecanoic acid (TA) as lipid components. The efficacy of these nanoparticles against S.A. was evaluated using orthogonal design analysis. FTIR, DLS, HPLC, and TEM analyses confirmed that tildipirosin was successfully incorporated into the solid lipid nanoparticles, resulting in an optimal nanoparticle drug delivery system with a particle size of 322.63 ± 1.51 nm, a zeta potential of 37.83 ± 0.95 mV, an encapsulation efficiency of 82.23 ± 0.45%, and a drug loading capacity of 7.36 ± 0.18%. The SLN-TD system exhibited high stability, effective sustained release <em>in vitro</em>, and enhanced intracellular activity against S.A. Pharmacokinetic studies in rats administered 4 mg kg<small>⁻¹</small><em>via</em> intramuscular and oral routes showed that, compared to unencapsulated tildipirosin (TD), SLN-TD provided sustained release <em>in vivo</em> and improved gastrointestinal absorption with higher bioavailability. Additionally, in a mouse model of S.A. infection, SLN-TD demonstrated superior antibacterial activity and sustained drug delivery for effective treatment. This study offers a promising multifunctional nanoparticle drug delivery system for the effective treatment of S.A. infections and enhances the oral bioavailability of tildipirosin, with potential applications in veterinary medicine.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 5","pages":" 1272-1285"},"PeriodicalIF":5.8,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coculture to vascularization transition in bioengineered skin grafts through VEGF-associated pathways tracked by exosomal biomarkers†","authors":"Shalini Dasgupta and Ananya Barui","doi":"10.1039/D4BM01544D","DOIUrl":"10.1039/D4BM01544D","url":null,"abstract":"<p >Inadequate vasculature poses a significant challenge in the clinical translation of tissue engineering constructs. Current strategies for vascularization typically recruit short-lived endothelial cells or induce mesenchymal stem cells (MSC) to differentiate into the endothelial lineage, often in combination with supporting pericytes or fibroblasts. However, endothelial-associated cocultures lack adaptive ability and form limited vasculature. In this study, we investigated the endothelial transdifferentiation of an MSC-fibroblast coculture loaded on a bioengineered graft and utilized the exosomes released by the coculture model as a biomarker to monitor the progress of vascularization inside the graft. To develop the pre-vascularized skin graft, dermal fibroblasts and MSC were seeded on a biocomposite chitosan/collagen/fibrinogen/D3 (CCF-D3) scaffold. The cocultured graft facilitated the differentiation of MSC to endothelial cells (MEnDoT). Additionally, it promoted vasculogenic sprouting through the VEGF–eNOS pathways, as evidenced by the expression of F-actin, VEGF-A, and downstream transcriptomic markers (CD31, CD34, eNOS, VEGF-A, VEGF-R2, PI3 K, and PLC-γ). Exosomes (∼130 nm diameter) were isolated from the coculture, and their spectral analysis revealed significant differences (<em>p</em> &lt; 0.05) in the intensity ratio of nucleotides (952 cm<small>⁻¹</small>), polysaccharides (1071 cm<small>⁻¹</small>) and lipoproteins (1417 cm<small>⁻¹</small>), corresponding to vasculogenesis. The activation of the VEGF-associated pathway in the coculture model was validated using an inhibitor (dexamethasone), which was used to treat the coculture graft as a control. Thus, this study elucidated the vascularization of coculture constructs <em>via</em> the VEGF-associated pathway. It demonstrated the potential of exosome spectral fingerprints as promising biomarkers to monitor the vascularization progression inside the graft, paving the way for the development of standardized grafts for full-thickness skin tissue regeneration.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 6","pages":" 1464-1481"},"PeriodicalIF":5.8,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143187828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cRGD-modified liposome for targeted delivery of artesunate to inhibit angiogenesis in endometriosis†","authors":"Jianyu Ma, Zhouzhou Liao, Jinbo Li, Xiao Li, Hongling Guo, Qiyu Zhong, Jianyun Huang, Xintao Shuai and Shuqin Chen","doi":"10.1039/D4BM01506A","DOIUrl":"10.1039/D4BM01506A","url":null,"abstract":"<p >Currently, hormonal therapy for endometriosis faces challenges in achieving a balance between treatment and preserving the chance of pregnancy. Therefore, the development of non-hormonal therapy holds significant clinical importance. Angiogenesis is a hallmark of endometriosis, and anti-angiogenic therapies targeting the hypoxia-inducible factor-1α (HIF-1α) pathway are considered potential approaches for endometriosis. However, angiogenesis is also involved in numerous physiological processes, including pregnancy, and systemic anti-angiogenesis may lead to severe adverse effects. To address this, a cRGD-modified liposome nanodrug (cRGD-LP-ART) is synthesized, which enhances drug efficacy while reducing adverse reactions. Artesunate (ART), a non-hormonal drug used to treat malaria, has shown anti-angiogenic effects beyond its original indications in various benign and malignant diseases. With cRGD modification, cRGD-LP-ART can target ectopic lesions and inhibit local angiogenesis by suppressing the HIF-1α/vascular endothelial growth factor (VEGF) pathway. Furthermore, cRGD-LP-ART exhibits better therapeutic effects than free ART, without affecting ovarian function or causing atrophy of the eutopic endometrium, making it a promising new option for non-hormonal therapy of endometriosis. As a combination of liposomes and a clinically approved drug, cRGD-LP-ART holds great potential and clinical prospects for the treatment of endometriosis.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 4","pages":" 1045-1058"},"PeriodicalIF":5.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and mechanisms of concentrated growth factor on facial nerve rehabilitation in a rabbit model†","authors":"Xiaochen Yang, Zhengyao Hou, Kexin Wang, Jieying Li, Wei Shang, Lin Wang and Kai Song","doi":"10.1039/D4BM01454E","DOIUrl":"10.1039/D4BM01454E","url":null,"abstract":"<p >Accelerated rehabilitation following facial nerve injury presents unique clinical challenges. This study evaluates the therapeutic effects of concentrated growth factor (CGF) on facial nerve recovery in a rabbit model and on RSC96 Schwann cells. Characterization of the CGF membrane (CGFM) revealed a three-dimensional fibrin network with embedded platelets, and representative growth factors, including TGF-β1, PDGF-BB, IGF-1, bFGF, and VEGF, were detected. <em>In vivo</em>, the Crush + CGFM group exhibited enhanced axon and myelin regeneration, increased Schwann cell proliferation, and improved facial nerve function compared to the Crush group. <em>In vitro</em>, CGF treatment significantly promoted the proliferation and migration of RSC96 cells and facilitated axon elongation in NG108-15 cells compared to controls. Mechanistically, CGF treatment led to a significant increase in PDGFRβ phosphorylation. Inhibition of this pathway with SU16f decreased Schwann cell activity and hindered overall nerve rehabilitation. These results underscore CGF's potential to accelerate nerve repair by promoting axon and myelin regeneration and enhancing Schwann cell biological activity, with the PDGFRβ pathway playing a crucial regulatory role. This study highlights CGF as a promising therapeutic strategy for improving facial nerve rehabilitation.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 4","pages":" 1059-1074"},"PeriodicalIF":5.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the endosomal escape of pH-responsive nanoparticles using the split luciferase endosomal escape quantification assay†","authors":"Umeka Nayanathara, Fan Yang, Changhe Zhang, Yufu Wang, Bruna Rossi Herling, Samuel A. Smith, Maximilian A. Beach, Angus P. R. Johnston and Georgina K. Such","doi":"10.1039/D4BM01433B","DOIUrl":"10.1039/D4BM01433B","url":null,"abstract":"<p >Endosomal escape is a major bottleneck for efficient intracellular delivery of therapeutic cargoes, particularly for macromolecular biological cargoes such as peptides, proteins and nucleic acids. pH-responsive polymeric nanoparticles that can respond to changes in the pH of intracellular microenvironments have generated substantial interest in navigating the endosomal barrier. In this study, we applied the highly sensitive split luciferase endosomal escape quantification (SLEEQ) assay to better understand the endosomal escape efficiency of dual component pH-responsive nanoparticles based on poly(2-(diethylamino) ethyl methacrylate) (PDEAEMA) and poly(2-(diisopropylamino) ethyl methacrylate) (PDPAEMA). Previous work investigated the use of a disulfide-linked HiBiT peptide conjugate encapsulated within the nanoparticle core, which upon meeting the LgBiT protein in the cytosol demonstrated luminescence which could be quantified to assess endosomal escape. However, we were interested in understanding whether this assay could be tuned to understand the endosomal escape of both a therapeutic cargo and a larger carrier. To achieve this, we designed two different HiBiT conjugates by applying a carbonylacrylic-functionalized thioether (non-cleavable) linker, which is more stable in endosomes, and a less stable disulfide (cleavable) linker to attach HiBiT to the nanoparticle core. Nanoparticles with disulfide-linked HiBiT demonstrated a higher endosomal escape efficiency of 6–7%, whereas thioether-linked HiBiT demonstrated &lt;3% endosomal escape efficiency with a twofold decrease in cytosolic delivery. This suggests that degradation of the disulfide linker in endosomes leads to cytosolic delivery of a free HiBiT cargo, while thioether-linked HiBiT polymers are larger and thus fewer HiBiT-carrier conjugates can escape the endosomes. Overall, this work demonstrates that the SLEEQ assay can be tuned to understand the cytosolic delivery of different components based on the use of different linker chemistries and thus it is an important tool for designing therapeutic delivery systems in the future.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 5","pages":" 1335-1346"},"PeriodicalIF":5.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-atom nanozymes with intelligent response to pathological microenvironments for bacterially infected wound healing†","authors":"Zhen Wan, Qingshan Liu, Yadong Zhe, Jiarong Li, Danqi Ding, Shuangjie Liu, Hao Wang, Huanhuan Qiao, Jiang Yang, Shaofang Zhang and Xiaoyu Mu","doi":"10.1039/D4BM01371A","DOIUrl":"10.1039/D4BM01371A","url":null,"abstract":"<p >Wound healing is a complex and dynamic process often accompanied by bacterial infection, inflammation, and excessive oxidative stress. Single-atom nanozymes with multi-enzymatic activities show significant potential for promoting the healing of infected wounds by modulating their antibacterial and anti-inflammatory properties in response to the wound's physiological environment. In this study, we synthesized MN<small>₄</small> single-atom nanozymes with multi-enzymatic activities that intelligently respond to pH value changes in the wound healing process. <em>In vitro</em> experiments confirm their effectiveness against Gram-negative bacteria, attributed to elevated reactive oxygen species (ROS) accumulation within the bacterial cells. Moreover, a full-thickness skin wound-infected model demonstrates that MN<small>₄</small> single-atom nanozymes accelerate wound repair and skin regeneration by suppressing the expression of tumor necrosis factor-alpha (TNF-α), promoting angiogenesis, and enhancing collagen deposition. <em>In vivo</em> biocompatibility experiments further demonstrate the favorable biocompatibility of these nanozymes, highlighting their potential for clinical applications in infected wound healing. These nanozymes respond intelligently to different microenvironments and may be suitable for addressing further complex and variable diseases.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 4","pages":" 1033-1044"},"PeriodicalIF":5.8,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}