Biomaterials Science最新文献_第10页

Polyvinyl alcohol/casein hydrogels with oxymatrine eluting ability for cancer-related wound management† 具有氧化苦参碱洗脱能力的聚乙烯醇/酪蛋白水凝胶用于癌症相关伤口处理。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-04-08 DOI: 10.1039/D5BM00191A

Pedro Rainho, Madalena Salema-Oom, Carlos A. Pinto, Jorge A. Saraiva, Benilde Saramago, Diana C. Silva and Ana Paula Serro

{"title":"Polyvinyl alcohol/casein hydrogels with oxymatrine eluting ability for cancer-related wound management†","authors":"Pedro Rainho, Madalena Salema-Oom, Carlos A. Pinto, Jorge A. Saraiva, Benilde Saramago, Diana C. Silva and Ana Paula Serro","doi":"10.1039/D5BM00191A","DOIUrl":"10.1039/D5BM00191A","url":null,"abstract":"Malignant fungating wounds (MFWs) are cancer-related complications that arise from metastases in advanced cancers. They appear in 5–14% of cancer patients, with higher prevalence in breast (66%) and head and neck (24%) cancers. Novel therapeutic routes for the management of MFWs rely on plant-based treatments, e.g. oxymatrine (OXM), an alkaloid derived from a Chinese plant with anticancer and anti-inflammatory properties. The objective of this work was to assess the potential of polyvinyl alcohol/casein (PVA/CAS) hydrogels to be used as dressings for OXM delivery. CAS can stimulate the immune system, while PVA is one of the most used synthetic polymers in the composition of hydrogels for medical applications. Six different hydrogel formulations were prepared following different procedures: freeze-thawing (FT) and cast drying (CD) for 24 or 48 h, with and without the addition of genipin (GE), a crosslinking agent. The hydrogels were loaded with OXM, and their release behaviour was studied. PVA/CAS-24CD + GE showed the best release profile. After being subjected to sterilisation by high hydrostatic pressure, it was further investigated in terms of physicochemical properties, mechanical characteristics and biocompatibility. Overall, this hydrogel revealed adequate characteristics to be used as a biocompatible medicated dressing for OXM release.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 10","pages":" 2755-2766"},"PeriodicalIF":5.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteria-responsive cytoderm drug delivery systems† 细菌反应性细胞壁给药系统。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-04-08 DOI: 10.1039/D5BM00026B

Chenmeng Zhou, Yue Zhang, Bo Tian, Yue Yu, Dongxiao Li, Bingbing Wu, Wenju Chang, Tongguo Shi, Fang Xu, Jinyu Bai and Chao Wang

引用次数: 0

Advances in bioink-based 3D printed scaffolds: optimizing biocompatibility and mechanical properties for bone regeneration 基于生物墨水的3D打印支架的进展：优化骨再生的生物相容性和机械性能。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-04-07 DOI: 10.1039/D4BM01606H

Pawan Kumar, Jitender Sharma, Ravinder Kumar, Jan Najser, Jaroslav Frantik, Anju Manuja, Nagaraju Sunnam and Seepana Praveenkumar

{"title":"Advances in bioink-based 3D printed scaffolds: optimizing biocompatibility and mechanical properties for bone regeneration","authors":"Pawan Kumar, Jitender Sharma, Ravinder Kumar, Jan Najser, Jaroslav Frantik, Anju Manuja, Nagaraju Sunnam and Seepana Praveenkumar","doi":"10.1039/D4BM01606H","DOIUrl":"10.1039/D4BM01606H","url":null,"abstract":"The development of bioink-based 3D-printed scaffolds has revolutionized bone tissue engineering (BTE) by enabling patient-specific and biomimetic constructs for bone regeneration. This review focuses on the biocompatibility and mechanical properties essential for scaffold performance, highlighting advancements in bioink formulations, material combinations, and printing techniques. The key biomaterials, including natural polymers (gelatin, collagen, alginate), synthetic polymers (polycaprolactone, polyethylene glycol), and bioactive ceramics (hydroxyapatite, calcium phosphate), are discussed concerning their osteoconductivity, printability, and structural integrity. Despite significant progress, challenges remain in achieving optimal mechanical strength, degradation rates, and cellular interactions. The review explores emerging strategies such as gene-activated bioinks, nanocomposite reinforcements, and crosslinking techniques to enhance scaffold durability and bioactivity. By synthesizing recent developments, this work provides insights into future directions for bioink-based scaffolds, paving the way for more effective and personalized bone regenerative therapies.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 10","pages":" 2556-2579"},"PeriodicalIF":5.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

20 nm nanoparticles trigger calcium influx to endothelial cells via a TRPV4 channel† 20nm纳米颗粒通过TRPV4通道触发钙流入内皮细胞。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-04-07 DOI: 10.1039/D4BM01691B

Jaspreet Singh Nagi and Amber L. Doiron

{"title":"20 nm nanoparticles trigger calcium influx to endothelial cells via a TRPV4 channel†","authors":"Jaspreet Singh Nagi and Amber L. Doiron","doi":"10.1039/D4BM01691B","DOIUrl":"10.1039/D4BM01691B","url":null,"abstract":"While increased intracellular calcium (Ca2+) has been identified as a key effect of nanoparticles on endothelial cells, the mechanism has not been fully elucidated or examined under shear stress. Here, we show the effect of several types of 20 nm particles on Ca2+ in the presence of shear stress in human umbilical vein endothelial cells (HUVECs), human coronary artery endothelial cells (HCAECs), and human cardiac microvascular endothelial cells (HMVEC-Cs). Intracellular Ca2+ levels increased by nearly three-fold in these cell types upon exposure to 100 μg mL−1 20 nm Au particles, which was not seen in response to larger or smaller particles. An antagonist to the calcium channel – transient receptor potential vanilloid-type 4 (TRPV4) – drastically reduced the amount of calcium by 9.3-fold in HUVECs exposed to 0.6 Pa shear stress and 100 μg mL−1 20 nm gold particles, a trend upheld in both HCAECs and HMVEC-Cs. Cell alignment in the direction of fluid flow is a well-known phenomenon in endothelial cells, and interestingly, cells in the presence of 20 nm particles with fluid flow had a higher alignment index than cells in the fluid flow alone. When compared with previous works, these results indicated that 20 nm particles may be inducing endothelial permeability by activating the TRPV4 channel in vitro. The potential of nanoparticle delivery technologies hinges on an improved understanding of this effect toward improved delivery with limited toxicity.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 10","pages":" 2728-2743"},"PeriodicalIF":5.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MnO2-based nanoparticles remodeling tumor micro-environment to augment sonodynamic immunotherapy against breast cancer† 二氧化锰纳米颗粒重塑肿瘤微环境，增强对乳腺癌的声动力免疫治疗。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-04-04 DOI: 10.1039/D5BM00189G

Haiqin Liao, Mingyu Chen, Zhipeng Liao, Yi Luo, Sijie Chen, Long Wang, Zhigang Wang and Chengcheng Niu

{"title":"MnO2-based nanoparticles remodeling tumor micro-environment to augment sonodynamic immunotherapy against breast cancer†","authors":"Haiqin Liao, Mingyu Chen, Zhipeng Liao, Yi Luo, Sijie Chen, Long Wang, Zhigang Wang and Chengcheng Niu","doi":"10.1039/D5BM00189G","DOIUrl":"10.1039/D5BM00189G","url":null,"abstract":"The tumor microenvironment (TME) is characterized by a complex array of factors, including aerobic conditions, high glutathione (GSH) levels, acidic pH, and elevated hydrogen peroxide (H2O2) content, all of which promote cancer progression and contribute to poor prognosis. Fortunately, these challenges can be addressed using MnO2-based nanomaterials. In this study, we have designed and synthesized a Curcumin/MnO2@PLGA@4T1 cell membrane (CMP@4T1m) system aimed at remodelling the TME and enhancing sonodynamic immunotherapy for breast cancer. Through the homologous targeting ability of 4T1m, CMP@4T1m efficiently accumulates at the tumor site. Upon ultrasound irradiation, curcumin (Cur) acts as a sonosensitizer, generating cytotoxic reactive oxygen species (ROS) that induce immunogenic cell death (ICD), activate T-cell responses, and repolarize protumoral M2-like macrophages to antitumoral M1-like macrophages. In the TME, which is mildly acidic and enriched with GSH and H2O2, MnO2 not only oxidizes GSH to glutathione disulfide (GSSG) but also reacts with H2O2 and H+ to produce oxygen, alleviating hypoxia and significantly enhancing the sonodynamic immunotherapy effect. Additionally, Mn2+ generated during this process converts H2O2 into cytotoxic hydroxyl radicals (˙OH). This study thus lays the foundation for advancing cancer nanomedicine, offering a novel approach that integrates TME remodelling with sonodynamic immunotherapy.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 10","pages":" 2767-2782"},"PeriodicalIF":5.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidics-driven templating preparation of polymer vesicles with tailorable dimensions and rapid cellular internalization† 微流体驱动模板制备具有可定制尺寸和快速细胞内化的聚合物囊泡。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-04-02 DOI: 10.1039/D5BM00377F

Donghua Dong, Tong Zhu, Guoxing Liao, Fangrong Tan, Lei Chen, Qianqian Yu and LinGe Wang

{"title":"Microfluidics-driven templating preparation of polymer vesicles with tailorable dimensions and rapid cellular internalization†","authors":"Donghua Dong, Tong Zhu, Guoxing Liao, Fangrong Tan, Lei Chen, Qianqian Yu and LinGe Wang","doi":"10.1039/D5BM00377F","DOIUrl":"10.1039/D5BM00377F","url":null,"abstract":"Polymer vesicles hold immense potential in biomedicine and nanotechnology, yet conventional rehydration methods face critical limitations in controlling the vesicle architecture due to stochastic block copolymer (BCP) self-assembly. Here, we present a first-reported microsphere-templated strategy that synergizes microfluidic precision with BCP assembly to overcome these constraints. By engineering emulsion templates via flow rate, BCP concentration and collection distance optimization, we established a method based on the radius-square law governing the evolution of uniform vesicles (size range diameter: 70–170 nm, PDI: 0.16), enabling on-demand size tuning, a capability unattainable with traditional approaches. Multi-scale characterization (DLS, OM, SEM and TEM) elucidates the non-equilibrium templating-to-vesicle transition, revealing critical dynamics of BCP film reorganization. The resultant nano-scale vesicles exhibit rapid cellular uptake (>95% in 3 h) by HUVECs and 4T1 cells with exceptional biocompatibility (>85% viability, 36 h), outperforming many cytotoxic counterparts. This work not only provides a scalable platform for precision vesicle fabrication but also establishes foundational principles for templated self-assembly, bridging microfluidics and soft matter science. Our methodology opens avenues for tailored vesicles in drug delivery, nanoreactors and synthetic biology, addressing the persistent demand for functionally adaptive polymeric nanostructures.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 11","pages":" 2925-2935"},"PeriodicalIF":5.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gradually-frozen aligned bacterial nanocellulose membranes loaded with gallic acid exhibit enhanced mechanical and dual antithrombotic-antimicrobial properties† 逐渐冷冻排列细菌纳米纤维素膜装载没食子酸表现出增强的机械和双重抗血栓抗菌性能。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-04-02 DOI: 10.1039/D5BM00176E

Emma D. Stephens, Fereshteh Oustadi, Hunter Marcelo, Jaqueline L. Vierra, Kartikeya Murari, Philip Egberts and Maryam Badv

{"title":"Gradually-frozen aligned bacterial nanocellulose membranes loaded with gallic acid exhibit enhanced mechanical and dual antithrombotic-antimicrobial properties†","authors":"Emma D. Stephens, Fereshteh Oustadi, Hunter Marcelo, Jaqueline L. Vierra, Kartikeya Murari, Philip Egberts and Maryam Badv","doi":"10.1039/D5BM00176E","DOIUrl":"10.1039/D5BM00176E","url":null,"abstract":"Bacterial nanocellulose (BNC) is a versatile natural polymer with unique morphological properties. However, its susceptibility to biofouling limits its utility in healthcare. To address this challenge, this study explores the incorporation of gallic acid, a phenolic acid with potent antimicrobial and antithrombotic properties, into BNC membranes. Additionally, a novel drying method termed gradual freezing is introduced, resulting in a directionally-aligned BNC membrane with enhanced mechanical integrity and high porosity. Using glycerol as a solvent and plasticizer, gallic acid was loaded into air-dried BNC (AD-BNC), freeze-dried BNC (FD-BNC), and gradually-frozen BNC (GF-BNC) membranes. Successful drug-loading into FD-BNC and GF-BNC significantly increased the elasticity of the films, however mechanical testing indicated that GF-BNC and its gallic acid/glycerol loaded counterpart (GF-GG-BNC) achieved overall optimal mechanical strength and elasticity. These samples were selected for further antifouling testing. Antibacterial assays demonstrated the practical efficacy of GF-GG-BNC in inhibiting the proliferation and biofilm formation of E. coli and S. aureus, while favorable antithrombotic behaviour prevented clot formation and red blood cell adhesion on the material's surface when compared to GF-BNC membranes. These findings highlight the potential of GF-GG-BNC as a multifunctional biomaterial for the prevention of biofouling in biomedical applications.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 10","pages":" 2673-2689"},"PeriodicalIF":5.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00176e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclen-based lipidoids for mRNA delivery and immunotherapy†

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-04-02 DOI: 10.1039/D5BM00317B

Lan Zhang, Ping Chen, Xiao-Li Tian, Yue Hu, Rong Wang and Ji Zhang

{"title":"Cyclen-based lipidoids for mRNA delivery and immunotherapy†","authors":"Lan Zhang, Ping Chen, Xiao-Li Tian, Yue Hu, Rong Wang and Ji Zhang","doi":"10.1039/D5BM00317B","DOIUrl":"https://doi.org/10.1039/D5BM00317B","url":null,"abstract":"As mRNA vaccines continue to gain widespread attention, the development of lipid nanoparticles (LNPs), as the preferred platform for mRNA delivery, has become a key focus of research. 1,4,7,10-Tetraazacyclododecane (cyclen), with its excellent protonation capability and ease of modification, has emerged as a promising candidate for the ionizable head group of lipid materials. In this study, a series of cyclen-based lipidoids with different linkages and hydrophobic tails was designed and conveniently synthesized. Structure–activity relationship studies were performed to screen out the carriers capable of efficient mRNA delivery and with potential for tumor therapeutic applications. In vivo biodistribution experiments in mice revealed that the lipidoid OEs-K, containing both hydroxyl and ester groups in its linkage, exhibited high mRNA delivery efficiency and lymph node-targeting properties. Using a subcutaneous EG.7-OVA tumor model in mice, the delivery of tumor antigen OVA mRNA using the lipidoid material was evaluated for its antitumor immunotherapeutic potential. Results demonstrated that LNPs formulated with OEs-K promoted dendritic cell uptake in lymph nodes, effectively activated immune responses, and inhibited tumor growth. Hematological and histopathological evaluations indicated no significant toxicity to the body. This study provides insights into the design and development of carrier materials for mRNA vaccines.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 10","pages":" 2783-2793"},"PeriodicalIF":5.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supervised machine learning for predicting drug release from acetalated dextran nanofibers†

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-03-28 DOI: 10.1039/D5BM00259A

Ryan N. Woodring, Elizabeth G. Gurysh, Tanvi Pulipaka, Kevin E. Shilling, Rebeca T. Stiepel, Erik S. Pena, Eric M. Bachelder and Kristy M. Ainslie

{"title":"Supervised machine learning for predicting drug release from acetalated dextran nanofibers†","authors":"Ryan N. Woodring, Elizabeth G. Gurysh, Tanvi Pulipaka, Kevin E. Shilling, Rebeca T. Stiepel, Erik S. Pena, Eric M. Bachelder and Kristy M. Ainslie","doi":"10.1039/D5BM00259A","DOIUrl":"https://doi.org/10.1039/D5BM00259A","url":null,"abstract":"Electrospun drug-loaded polymeric nanofibers can improve the efficacy of therapeutics for a variety of implications. By design, these biomaterial platforms can enhance drug bioavailability and site-specific delivery while reducing off-target toxicities when compared to other conventional formulations. By incorporating biocompatible and biodegradable polymers with tunable degradation rates, such as acetalated dextran (Ace-DEX), drug-loaded nanofibers can enhance the safety and efficacy of treatment regimens while improving patient compliance through controlled release. Despite these benefits, clinical translation of electrospun formulations is challenged by labor-intensive in vitro studies for ensuring that release kinetics are accurately characterized and reproducible. In this study, we report a novel workflow for assessing in vitro drug release from Ace-DEX nanofibers using machine learning (ML) and develop a predictive model to streamline this rate-limiting step. The developed Gaussian process regression (GPR) model was trained, validated, and optimized using in vitro release profiles from thirty electrospun Ace-DEX scaffolds. The results of GPR model simulations reveal consistent performance across all Ace-DEX formulations considered in this study while also demonstrating a drug-agnostic approach to predict fractional drug release over time.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 10","pages":" 2806-2823"},"PeriodicalIF":5.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00259a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term interleukin-4 release from 3D printable affinity hydrogels promotes M2-like macrophage polarisation in vitro† 3D打印亲和水凝胶长期释放白细胞介素-4促进体外m2样巨噬细胞极化。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-03-28 DOI: 10.1039/D4BM01623H

George A. Loxley, Consuelo Coser, Amir M. Ghaemmaghami and Jing Yang

{"title":"Long-term interleukin-4 release from 3D printable affinity hydrogels promotes M2-like macrophage polarisation in vitro†","authors":"George A. Loxley, Consuelo Coser, Amir M. Ghaemmaghami and Jing Yang","doi":"10.1039/D4BM01623H","DOIUrl":"10.1039/D4BM01623H","url":null,"abstract":"The biopharmaceutical industry for engineered protein drugs is rapidly increasing in size but there is a lack of controlled release vehicles to enable targeted delivery for regenerative medicine applications. In this study, we used photocrosslinkable 3-sulfopropyl acrylate potassium salt (SPAK)–poly(ethylene glycol) diacrylate (PEGDA) hydrogels to achieve controlled release of lysozyme for 70 days with zero-order release and tuneable release rate. Scaling down hydrogel volume and protein loading concentration to release Transforming growth factor beta-1 (TGF-β1) and Interleukin-4 (IL-4) resulted in low cumulative release, even without SPAK. Increasing PEGDA molecular weight from 4 kDa to 20 kDa improved TGF-β1 release but it still remained below 10% after 10 days. We observed sustained IL-4 release in the therapeutic ng mL−1 range for 73 days when loading IL-4 to 5% SPAK–10% PEGDA post photocrosslinking. Released IL-4 maintained bioactivity, promoting M2-like polarisation of THP-1 macrophages with day 53 supernatant, modelling long-term immunomodulation in vitro. We manufactured SPAK–PEGDA hydrogels by projection micro stereolithography, in which 3D printed 5% SPAK–10% PEGDA had an increased lysozyme release rate compared to its cast counterpart. 3D printed 5% SPAK–10% PEGDA with porous 3D design had an increased lysozyme release rate compared to a volume matched non-porous design. These findings highlight the potential of SPAK–PEGDA hydrogels for long-term cytokine delivery and show proof-of-concept for manipulating protein release kinetics with 3D printed hydrogel design.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 9","pages":" 2489-2502"},"PeriodicalIF":5.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11951857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0