Biomaterials Science最新文献

{"title":"Influence of viscosity on adipogenic and osteogenic differentiation of mesenchymal stem cells during 2D culture†","authors":"Chengyu Lu, Tianjiao Zeng, Man Wang, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang and Guoping Chen","doi":"10.1039/D4BM00710G","DOIUrl":"10.1039/D4BM00710G","url":null,"abstract":"<p >Accumulatively, cellular behaviours triggered by biochemical cues have been widely explored and the focus of research is gradually shifting to biophysical cues. Compared to physical parameters such as stiffness, substrate morphology and viscoelasticity, the influence of viscosity on cellular behaviours is relatively unexplored and overlooked. Thus, in this study, the influence of viscosity on the adipogenic and osteogenic differentiation of human mesenchymal stem cells (hMSCs) was investigated by adjusting the viscosity of the culture medium. Viscosity exhibited different effects on adipogenic and osteogenic differentiation of hMSCs during two-dimensional (2D) culture. High viscosity facilitated osteogenic while inhibiting adipogenic differentiation. During adipogenic differentiation, the effect of viscosity on cell proliferation was negligible. However, during osteogenic differentiation, high viscosity decreased cell proliferation. The different influence of viscosity could be explained by the activation of mechanotransduction regulators of Yes-associated protein (YAP) and β-catenin. High viscosity could promote YAP and β-catenin nuclear translocation during osteogenic differentiation, which was responsible for the increased osteogenesis. High viscosity inhibited adipogenesis through promoting YAP nuclear translocation. This study could broaden the understanding of how viscosity can affect stem cell differentiation during 2D culture, which is valuable for tissue engineering.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 21","pages":" 5598-5609"},"PeriodicalIF":5.8,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different leukocyte subsets are targeted by systemic and locoregional administration despite conserved nanomaterial characteristics optimal for lymph node delivery†","authors":"Paul A. Archer, Alexander J. Heiler, Alisyn R. Bourque, Yunus Alapan and Susan N. Thomas","doi":"10.1039/D4BM00910J","DOIUrl":"10.1039/D4BM00910J","url":null,"abstract":"<p >Lymph nodes (LNs) house a large proportion of the body's leukocytes. Accordingly, engineered nanomaterials are increasingly developed to direct therapeutics to LNs to enhance their efficacy. Yet while lymphatic delivery of nanomaterials to LNs upon locoregional injection has been extensively evaluated, nanomaterial delivery to LN-localized leukocytes after intravenous administration has not been systematically explored nor benchmarked. In this work, a panel of inert, fluorescent nanoscale tracers and drug delivery vehicles were utilized to interrogate intravenous <em>versus</em> locoregionally administered nanomaterial access to LNs and leukocyte subsets therein. Hydrodynamic size and material effects on LN accumulation extents were similar between intravenous <em>versus</em> intradermal injection routes. Nanomaterial distribution to various LN leukocyte subsets differed substantially with injection route, however, in a manner not proportional to total LN accumulation. While intravenously administered nanomaterials accumulated in LNs lowly compared to systemic tissues, in sharp contrast to locoregional delivery, they exhibited size-dependent but material-independent access to immune cells within the LN parenchyma, which are not easily accessed with locoregional delivery.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 21","pages":" 5582-5597"},"PeriodicalIF":5.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/bm/d4bm00910j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing 3D printed highly porous Ti–6Al–4V scaffolds coated with graphene oxide to promote osteogenesis","authors":"Hee Jeong Jang, Moon Sung Kang, Jinju Jang, Dohyung Lim, Seong-Won Choi, Tae-Gon Jung, Heoung-Jae Chun, Bongju Kim and Dong-Wook Han","doi":"10.1039/D4BM00970C","DOIUrl":"10.1039/D4BM00970C","url":null,"abstract":"<p >Bone tissue engineering (BTE) strategies have been developed to address challenges in orthopedic and dental therapy by expediting osseointegration and new bone formation. In this study, we developed irregular porous Ti–6Al–4V scaffolds coated with reduced graphene oxide (rGO), specifically rGO-pTi, and investigated their ability to stimulate osseointegration <em>in vivo</em>. The rGO-pTi scaffolds exhibited unique irregular micropores and high hydrophilicity, facilitating protein adsorption and cell growth. <em>In vitro</em> assays revealed that the rGO-pTi scaffolds increased alkaline phosphatase (ALP) activity, mineralization nodule formation, and osteogenic gene upregulation in MC3T3-E1 preosteoblasts. Moreover, <em>in vivo</em> transplantation of rGO-pTi scaffolds in rabbit calvarial bone defects showed improved bone matrix formation and osseointegration without hemorrhage. These findings highlight the potential of combining rGO with irregular micropores as a promising BTE scaffold for bone regeneration.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 21","pages":" 5491-5503"},"PeriodicalIF":5.8,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alginate/GelMA microparticles via oil-free interface shearing for untethered magnetic microbots†","authors":"Shiyu Wu, Yang Zhou, Juan Wei, Zicheng Da, Wenquan Chen, Xiaoxia Shu, Tingting Luo, Yuping Duan, Runhuai Yang, Chengbiao Ding and Guangli Liu","doi":"10.1039/D4BM00875H","DOIUrl":"10.1039/D4BM00875H","url":null,"abstract":"<p >Microrobots hold broad application prospects in the field of precision medicine, such as intravenous drug injection, tumor resection, opening blood vessels and imaging during abdominal surgery. However, the rapid and controllable preparation of biocompatible hydrogel microparticles still poses challenges. This study proposes the one-step direct acquisition of biocompatible sodium alginate and gelatin methacrylate (GelMA) hydrogel microparticles using an oil-free aqueous solution, ensuring production with a controllable generation frequency. An adaptive interface shearing platform is established to fabricate alginate/GelMA microparticles using a mixture of the hydrogel, photoinitiator, and Fe<small>₃</small>O<small>₄</small> nanoparticles (NPs). By adjusting the static magnetic field intensity (<em>B</em><small>_s</small>), vibration frequency, and flow rate (<em>Q</em>) of the dispersed phase, the size and morphology of the hydrogel microparticles can be controlled. These hydrogel microparticle robots exhibit magnetic responsiveness, demonstrating precise rotating and rolling movements under the influence of an externally rotating magnetic field (RMF). Moreover, hydrogel microparticle robots with a specific critical frequency (<em>C</em><small>_f</small>) can be customized by adjusting the <em>B</em><small>_s</small> and the concentration of Fe<small>₃</small>O<small>₄</small> NPs. The directional <em>in situ</em> untethered motion of the hydrogel microparticle robots can be successfully realized and accurately controlled in the climbing over obstacles and <em>in vitro</em> experiments of animals, respectively. This versatile and fully biodegradable microrobot has the potential to precisely control movement to bone tissue and the natural cavity of the human body, as well as drug delivery.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 21","pages":" 5562-5572"},"PeriodicalIF":5.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphitic carbon nitride as a novel anticancer agent: potential mechanisms and efficacy in prostate cancer and glioblastoma treatment","authors":"Natalia Yoshihara, Michelle Lopes, Isabel Santos, Beatriz Kopke, Clara Almeida, Joyce Araújo, Pierre B. A. Fechine, Ralph Santos-Oliveira and Celso Sant'Anna","doi":"10.1039/D4BM01025F","DOIUrl":"10.1039/D4BM01025F","url":null,"abstract":"<p >Carbon-derived compounds are gaining traction in the scientific community because of their unique properties, such as conductivity and strength, and promising innovations in technology and medicine. Graphitic nitride carbon (g-C<small>₃</small>N<small>₄</small>) stands out among these compounds because of its potential in antitumor therapies. This study aimed to assess g-C<small>₃</small>N<small>₄</small>'s antitumor potential and cytotoxic mechanisms. Prostate cancer (DU-145) and glioblastoma (U87) cell lines were used to evaluate antitumor effects, whereas RAW 264.7 and HFF-1 non-tumor cells were used for selectivity evaluation. The synthesized g-C<small>₃</small>N<small>₄</small> particles underwent comprehensive characterization, including the assessment of particle size, morphology, and oxygen content, employing various techniques, such as X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy, and atomic force microscopy. The results indicated that g-C<small>₃</small>N<small>₄</small> significantly affected tumor cell proliferation and viability, exhibiting high cytotoxicity within 48 h. In non-tumor cells, minimal effects on proliferation were observed, except for damage to the cell membranes of RAW 264.7 cells. Moreover, g-C<small>₃</small>N<small>₄</small> changed the cell morphology and ultrastructure, affecting cell migration in U87 cells and potentially enhancing migration in RAW 264.7 cells. Biochemical assays in Balb/C mice revealed alterations in alanine aminotransferase, aspartate aminotransferase, and amylase levels. In conclusion, g-C<small>₃</small>N<small>₄</small> demonstrated promising antitumor effects with minimal toxicity to non-tumor cells, suggesting its potential in neoplasm treatment.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 21","pages":" 5547-5561"},"PeriodicalIF":5.8,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polysorbate 80-containing ionizable lipid nanoparticles for mRNA delivery†","authors":"Xuefeng Tang, Shixiao Ding, Shilin Yang, Yuqiao Cheng, Hanyu Liu, Kexin Chen and Xiaojun Han","doi":"10.1039/D4BM00523F","DOIUrl":"10.1039/D4BM00523F","url":null,"abstract":"<p >Ionizable lipid nanoparticles have demonstrated remarkable success as mRNA vaccine carriers and represent one of the most promising gene drug delivery vehicles. However, polyethylene glycol (PEG), one of the major components, can cause immunogenic reactions, anaphylaxis and increased blood clearance, leading to toxic side effects and reduced efficacy. In this study, we utilize polysorbate 80 (PS80) as a PEG alternative in formulating eGFP mRNA-loaded ionizable lipid nanoparticles (PS80-iLNPs), aiming to enhance stealth properties, uptake efficiency, and biosafety. Our findings revealed that PS80-iLNPs enhanced the stealthiness and resistance to serum interference. Compared to PEG-containing ionizable lipid nanoparticles (PEG-iLNPs), PS80-iLNPs showed a 1.14-fold increase in stealthiness. Moreover, at a total lipid concentration of 50 μg mL<small>⁻¹</small>, PS80-iLNPs exhibited 1.12 times higher cell viability compared to PEG-iLNPs. Notably, under serum interference, PEG-iLNPs showed a 44.97% uptake reduction, whereas PS80-iLNPs exhibited a modest 30.55% decrease, underscoring its superior serum resistance. This work demonstrated that PS80 could serve as a suitable substitute for PEG, thus signifying an excellent basis for the development of PEG-free ionizable lipid nanoparticles.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 21","pages":" 5573-5581"},"PeriodicalIF":5.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The case for poly(ylides) as a class of charge-neutral, hydrophilic polymers with applications in biomaterials science","authors":"Kevin Neumann","doi":"10.1039/D4BM00928B","DOIUrl":"10.1039/D4BM00928B","url":null,"abstract":"<p >Many applications of biomaterials require hydrophilic polymers as building blocks, including hydrogels and nanomedicinal devices. Besides enabling sufficient swelling properties in aqueous environments, hydrophilic polymers provide hydration layers, which are considered a major requirement when designing non-fouling surfaces and materials. For the last few decades, polyethylene glycol has been seen as the gold standard for such applications. However, reports on its stability and immunogenicity have urged chemists to identify alternatives with comparable or superior properties. In addition to biopolymers, zwitterionic polymers have gained increasing attention by effectively offering an overall charge-neutral scaffold capable of forming strong hydration layers. Driven by an enhanced understanding of the structure–property relationship of zwitterionic materials, poly(ylides) have emerged as a new class of hydrophilic and charge-neutral polymers. By having the negative charge adjacent to the positive charge, ylides offer not only a minimal dipole moment but also maintain their overall charge-neutral nature. Despite some early reports on their synthesis during the 1980s, polymeric ylides were largely overlooked as a class of polymers, and their utility as unique hydrophilic building blocks for the design of biomaterials and nanomedicinal tools remained elusive. In recent years, several groups have reported <em>N</em>-oxide and carbon-centered ylide-based polymers as highly effective building blocks for the design of antifouling materials and nanomedicines. Here, by reviewing recent progress and understanding of structure–property relationships, arguments are provided explaining why polymeric ylides should be classified as a standalone class of hydrophilic polymers. Consequently, the author concludes that the term ‘poly(ylide)’ or ‘polymeric ylides’ should be routinely used to adequately describe this emerging class of polymers.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 21","pages":" 5481-5490"},"PeriodicalIF":5.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/bm/d4bm00928b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic hemostatic silk fibroin film with photo-controllable modulation on macrophage for bacteria infected wound healing","authors":"Xiaoxuan Tang, Wenpin Wu, Shuxuan Zhang, Chang He, Kewei Fan, Yulan Fan, Xuewa Yang, Jiaying Li, Yumin Yang, Jue Ling","doi":"10.1039/d4bm01038h","DOIUrl":"https://doi.org/10.1039/d4bm01038h","url":null,"abstract":"Massive hemorrhage and chronic wounds caused by bacterial infections after trauma have always been significant challenges in clinical practice. An ideal hemostatic wound dressing should not only simultaneously manage bleeding and prevent bacterial infections, but also holds excellent biocompatibility and bioactivities to successfully modulate immune microenvironments to promote wound healing. Previously, a silk fibroin based light responsive film was successfully prepared. In this study, the silk fibroin film was demonstrated to possess effective capacity of light-induced non-compressible hemostasis on liver hemorrhage and tail bleeding in vivo by binding with blood platelets to promote the clotting cascade. Significantly, the films exhibited photo-controllable modulation activity on macrophage through repeated near-infrared irradiation to regulate the immune microenvironment to enhance photodynamic antibacterial therapy. Moreover, the light responsive silk fibroin film effectively promoted Staphylococcus aureus infected burn wound healing in vivo, providing a powerful strategy for wound healing of burns.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":"7 1","pages":""},"PeriodicalIF":6.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant activities of metal single-atom nanozymes in biomedicine","authors":"Qingdong Zeng, Huihai Zhong, Jiahao Liao, Qin Huo, Beiping Miao, Li Zeng, Bin Zhang and Guohui Nie","doi":"10.1039/D4BM00978A","DOIUrl":"10.1039/D4BM00978A","url":null,"abstract":"<p >Nanozymes are a class of nanomaterials with enzyme-like activity that can mimic the catalytic properties of natural enzymes. The small size, high catalytic activity, and strong stability of nanozymes compared to those of natural enzymes allow them to not only exist in a wide temperature and pH range but also maintain stability in complex environments. Recently developed single-atom nanozymes have metal active sites composed of a single metal atom fixed to a carrier. These metal atoms can act as independent catalytically active centers. Metal single-atom nanozymes have a homogeneous single-atom structure and a suitable coordination environment for stronger catalytic activity and specificity than traditional nanozymes. The antioxidant metal single-atom nanozymes with the ability of removing reactive oxygen species (ROS) can simulate superoxidase dismutase, catalase, and glutathione peroxidase to show different effects <em>in vivo</em>. Furthermore, due to the similar structure of antioxidant enzymes, a metal single-atom nanozyme often has multiple antioxidant activities, and this synergistic effect can more efficiently remove ROS related to oxidative stress. The versatility of single-atom nanozymes encompasses a broad spectrum of biomedical applications such as anti-oxidation, anti-infection, immunomodulatory, biosensing, bioimaging, and tumor therapy applications. Herein, the nervous, circulatory, digestive, motor, immune, and sensory systems are considered in order to demonstrate the role of metal single-atom nanozymes in biomedical antioxidants.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 20","pages":" 5150-5163"},"PeriodicalIF":5.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemp sprout-derived exosome-like nanovesicles as hepatoprotective agents attenuate liver fibrosis†","authors":"Ji-Su Kim, Jung-Young Eom, Hyun-Woo Kim, Je-Won Ko, Eui-Ju Hong, Mun-Nyeon Kim, Jihoon Kim, Do-Kyun Kim, Hyo-Jung Kwon and Young-Eun Cho","doi":"10.1039/D4BM00812J","DOIUrl":"10.1039/D4BM00812J","url":null,"abstract":"<p >Non-alcoholic fatty liver disease (NAFLD) is a form of hepatic steatosis in which more than 5% of the liver's weight is fat, primarily due to the overconsumption of soft drinks and a Western diet. In this study, we investigate the potential of plant-derived exosome-like nanovesicles (PENs) to prevent liver fibrosis and leaky gut resulting from NAFLD. Specifically, we examine whether hemp sprout-derived exosome-like nanovesicles (HSNVs) grown on smart farms could exert protective effects against NAFLD by inhibiting liver fibrosis. HSNVs ranging from 100–200 nm were measured using nanoparticle tracking analysis (NTA). HSNVs (1 mg kg<small>⁻¹</small>) were orally administered for 5 weeks to mice with NAFLD induced by feeding them a Western diet (WD; a fat- and cholesterol-rich diet) and fat-, fructose-, and cholesterol-rich (FFC) diet for 8 weeks. Importantly, the administration of HSNVs markedly reduced oxidative stress and fibrosis marker proteins in NAFLD mouse models and LX2 cells. Furthermore, treatment with HSNVs prevented a significant decrease in the quantity of gut barrier proteins and endotoxin levels in NAFLD mouse models. For the first time, these results demonstrate that HSNVs can exhibit a hepatoprotective effect against gut leakiness and WD/FFC-induced liver fibrosis by inhibiting oxidative stress and reducing fibrosis marker proteins.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 20","pages":" 5361-5371"},"PeriodicalIF":5.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}