Biomaterials Science最新文献_第9页

Engineering a bacterial polysaccharide-based metal–organic framework-enhanced bioactive 3D hydrogel for accelerated full-thickness wound healing† 设计一种基于细菌多糖的金属有机框架增强生物活性3D水凝胶，用于加速全层伤口愈合。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-09 DOI: 10.1039/D5BM00133A

Aniruddha Dan, Ankita Panigrahi, Hemant Singh, Varsha Murali, Manisha Meena, Prateek Goyel, Laxmanan Karthikeyan, Superb K. Misra, Nibu Varghese, Sharlene Sara Babu, Yogesh B. Dalvi and Mukesh Dhanka

{"title":"Engineering a bacterial polysaccharide-based metal–organic framework-enhanced bioactive 3D hydrogel for accelerated full-thickness wound healing†","authors":"Aniruddha Dan, Ankita Panigrahi, Hemant Singh, Varsha Murali, Manisha Meena, Prateek Goyel, Laxmanan Karthikeyan, Superb K. Misra, Nibu Varghese, Sharlene Sara Babu, Yogesh B. Dalvi and Mukesh Dhanka","doi":"10.1039/D5BM00133A","DOIUrl":"10.1039/D5BM00133A","url":null,"abstract":"Hydrogels offer numerous advantages in wound healing, making them a promising alternative to traditional wound dressings. Their biocompatibility and high water content closely resemble natural biological tissues, creating a moist environment that accelerates cell proliferation and tissue repair. Hydrogels maintain optimal hydration levels, preventing wound desiccation and promoting faster healing. Furthermore, their ability to incorporate and deliver therapeutic agents such as antibiotics, anti-inflammatory drugs, or growth factors provides a multifunctional platform for enhanced wound care. Despite these advantages, current clinical wound-dressing materials often fall short in addressing the complexities of wound healing. Hydrogels, with their customizable properties and potential for integration with emerging technologies, represent a significant opportunity to overcome these limitations and improve clinical outcomes in wound management. Herein, we developed a multi-functional Cu-MOF and tannic acid-enriched polymeric hydrogel dressing composed of gellan-gum/zein for full-thickness wound repair. The physical interactions, including electrostatic interaction and hydrogen bonding between the hydrogel components, form a stable hydrogel matrix. The hydrogel exhibits antioxidant properties and antibacterial activity, and is hemocompatible and biocompatible against L929 fibroblast cells. Furthermore, the fabricated hydrogel dressing improvised a full-thickness wound-healing process in rats. Only 1.6% of the wound area was remaining in the case of GG-Z-TA/M1-treated full-thickness wounds in rats. Histopathology images suggest the Cu-MOF-loaded hydrogels aided in extensive re-epithelialization, neovascularization, and hair follicle formation to accelerate the wound-healing process.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 15","pages":" 4107-4122"},"PeriodicalIF":5.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00133a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a nano-vaccine for high-grade serous ovarian cancer† 高级别浆液性卵巢癌纳米疫苗的研制

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-08 DOI: 10.1039/D4BM01696C

Chayanika Saha, Ahmed Elkashif, Elaine J. Gilmore, Binyumeng Jiang, Ying Sun, Raj Kumar Duary, Niamh Buckley, Nicholas J. Dunne and Helen O. McCarthy

{"title":"Development of a nano-vaccine for high-grade serous ovarian cancer†","authors":"Chayanika Saha, Ahmed Elkashif, Elaine J. Gilmore, Binyumeng Jiang, Ying Sun, Raj Kumar Duary, Niamh Buckley, Nicholas J. Dunne and Helen O. McCarthy","doi":"10.1039/D4BM01696C","DOIUrl":"10.1039/D4BM01696C","url":null,"abstract":"High-Grade Serous Carcinoma (HGSC) is characterised by aggressive malignant tumours and poor prognosis accounting for 75% of ovarian cancer. Conventional treatments often result in relapse, with a need for innovative therapeutic approaches. This study aimed to develop and evaluate a DNA vaccine targeting the preferentially expressed antigen of melanoma, PRAME, a cancer tumour antigen (CTA) overexpressed in HGSC. PRAME demonstrated the highest differential gene expression between normal fallopian tubes and HGSC tumour tissues in a range of patient datasets. The PRAME DNA was condensed by the cationic cell-penetrating peptide RALA to form nanoparticles (NPs). These self-assembling NPs exhibited a mean hydrodynamic size <150 nm and zeta potential >10 mV at N : P ratios ≥4 with ≤3% free DNA. The NPs successfully transfected NCTC-929 and DC 2.4 cells with PRAME overexpression, with negligible cytotoxicity. Vaccination with the NPs in vivo elevated CD4+ and CD8+ T-cell activation with increased expression of INF-γ and IL-2 cytokines. Vaccination also significantly improved survival rates in a PRAME-expressing tumour model in vivo. This study demonstrated the utility of a PRAME-targeted DNA vaccine for HGSC treatment which warrants further investigation.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 11","pages":" 2908-2924"},"PeriodicalIF":5.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d4bm01696c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tunable hydrogel networks by varying secondary structures of hydrophilic peptoids provide viable 3D cell culture platforms for hMSCs† 通过改变亲水性肽的二级结构可调的水凝胶网络为hMSCs提供了可行的3D细胞培养平台。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-07 DOI: 10.1039/D5BM00433K

Aldaly Pineda-Hernandez, David A. Castilla-Casadiego, Logan D. Morton, Sebastian A. Giordano-Nguyen, Kathleen N. Halwachs and Adrianne M. Rosales

{"title":"Tunable hydrogel networks by varying secondary structures of hydrophilic peptoids provide viable 3D cell culture platforms for hMSCs†","authors":"Aldaly Pineda-Hernandez, David A. Castilla-Casadiego, Logan D. Morton, Sebastian A. Giordano-Nguyen, Kathleen N. Halwachs and Adrianne M. Rosales","doi":"10.1039/D5BM00433K","DOIUrl":"10.1039/D5BM00433K","url":null,"abstract":"Hydrogels have excellent ability to mimic the extracellular matrix (ECM) during 3D cell culture, yet it remains difficult to tune their mechanical properties without also changing network connectivity. Previously, we developed 2D culture platforms based on tunable hydrogels crosslinked by peptoids with various secondary structures: helical, non-helical, and unstructured, which allowed control over hydrogel mechanics independent of network connectivity. Here, we extend our strategy to 3D matrices by modifying the peptoids with piperazine and homopiperazine residues to enhance water solubility without altering their secondary structure. Hydrogels crosslinked with helical peptoids exhibited significantly higher stiffness compared to hydrogels crosslinked with non-helical or unstructured peptoids. Human mesenchymal stem cells (hMSCs) encapsulated within these hydrogels were assessed for viability, proliferation, and immunomodulatory potential. The stiffest hydrogels promoted the highest rates of proliferation and increased yes-associated protein (YAP) nuclear localization. Softer hydrogels, however, showed enhanced production of indoleamine 2,3-dioxygenase (IDO), both with and without interferon gamma (IFN-γ) stimulation, highlighting their potential in immunomodulatory applications. The biomimetic platform developed here enables the study of how matrix mechanics influence stem cell behavior without confounding factors from network connectivity, leading to insights for hMSC-mediated immunomodulation.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3380-3394"},"PeriodicalIF":5.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Urethral reconstruction using an amphiphilic tissue-engineered autologous polyurethane nanofiber scaffold with rapid vascularization function 更正：使用具有快速血管化功能的两亲性组织工程自体聚氨酯纳米纤维支架进行尿道重建。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-07 DOI: 10.1039/D5BM90034D

Yuqing Niu, Guochang Liu, Chuangbi Chen, Ming Fu, Wen Fu, Zhang Zhao, Huimin Xia and Florian J. Stadler

引用次数: 0

Development of a bioactive hyaluronic acid hydrogel functionalised with antimicrobial peptides for the treatment of chronic wounds† 生物活性透明质酸水凝胶的开发与抗菌肽功能化治疗慢性伤口。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-07 DOI: 10.1039/D5BM00567A

Noémie Petit, Ana Gomes, Yu-yin Joanne Chang, Jessica Da Silva, Ermelindo C. Leal, Eugénia Carvalho, Paula Gomes and Shane Browne

{"title":"Development of a bioactive hyaluronic acid hydrogel functionalised with antimicrobial peptides for the treatment of chronic wounds†","authors":"Noémie Petit, Ana Gomes, Yu-yin Joanne Chang, Jessica Da Silva, Ermelindo C. Leal, Eugénia Carvalho, Paula Gomes and Shane Browne","doi":"10.1039/D5BM00567A","DOIUrl":"10.1039/D5BM00567A","url":null,"abstract":"Chronic wounds present significant clinical challenges due to delayed healing and high infection risk. This study presents the development and characterisation of acrylated hyaluronic acid (AcHyA) hydrogels functionalised with gelatin (G) and the antimicrobial peptide (AMP) PP4-3.1 to enhance cellular responses while providing antimicrobial activity. AcHyA-G and AcHyA-AMP hydrogels were formed via thiol–acrylate crosslinking, enabling in situ AcHyA hydrogel formation with stable mechanical properties across varying gelatin concentrations. Biophysical characterisation of AcHyA-G hydrogels showed rapid gelation, elastic behaviour, uniform mesh size, and consistent molecular diffusion across all formulations. Moreover, the presence of gelatin enhanced stability without affecting the hydrogel's degradation kinetics. AcHyA-G hydrogels supported the adhesion and spreading of key cell types involved in wound repair (dermal fibroblasts and endothelial cells), with 0.5% gelatin identified as the optimal effective concentration. Furthermore, the conjugation of the AMP conferred bactericidal activity against Staphylococcus aureus and Escherichia coli, two of the most prevalent bacterial species found in chronically infected wounds. These results highlight the dual function of AcHyA-AMP hydrogels in promoting cellular responses and antimicrobial activity, offering a promising strategy for chronic wound treatment. Further in vivo studies are needed to evaluate their efficacy, including in diabetic foot ulcers.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 13","pages":" 3561-3575"},"PeriodicalIF":5.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00567a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating green solvents for sustainable PLGA nanoparticle synthesis† 评价可持续合成PLGA纳米颗粒的绿色溶剂。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-07 DOI: 10.1039/D5BM00374A

Senjuti Karmaker, Rhea Joshi, Amartya Viravalli and Natalie Boehnke

引用次数: 0

CRISPR-based genetically modified scaffold-free biomaterials for tissue engineering and regenerative medicine 用于组织工程和再生医学的基于crispr的转基因无支架生物材料。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-06 DOI: 10.1039/D5BM00194C

Yunxuan Chen, Ke Yu, Zhiwei Jiang and Guoli Yang

引用次数: 0

Hydrogel-mediated delivery of baicalein for the effective therapy of MRSA-infected diabetic wounds by immune response and moderate photothermal effects† 水凝胶介导的黄芩苷递送通过免疫反应和适度光热效应有效治疗mrsa感染的糖尿病伤口。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-06 DOI: 10.1039/D5BM00335K

Yan Wang, Suyuan Su, Chaofeng Wang, Congyang Mao, Xiangmei Liu, Hanpeng Liu, Zhaoyang Li, Shengli Zhu, Hui Jiang, Zhenduo Cui, Yufeng Zheng and Shuilin Wu

{"title":"Hydrogel-mediated delivery of baicalein for the effective therapy of MRSA-infected diabetic wounds by immune response and moderate photothermal effects†","authors":"Yan Wang, Suyuan Su, Chaofeng Wang, Congyang Mao, Xiangmei Liu, Hanpeng Liu, Zhaoyang Li, Shengli Zhu, Hui Jiang, Zhenduo Cui, Yufeng Zheng and Shuilin Wu","doi":"10.1039/D5BM00335K","DOIUrl":"10.1039/D5BM00335K","url":null,"abstract":"Persistent bacterial infections and the imbalance in immune regulation induced by oxidative stress present a significant challenge in diabetic wound healing. In this study, we developed a novel dual-network hydrogel system composed of chitosan/polyacrylic acid (PEC) and polyoxometalate PMo12 (PMo12-PEC) loaded with baicalein (BA), designated as PMo12-BA-PEC, for the treatment of methicillin-resistant Staphylococcus aureus (MRSA)-infected diabetic wounds. The hydrogel demonstrated enhanced mechanical strength and elasticity, facilitating effective wound adherence and accommodating tissue movement. Upon 808 nm near-infrared (NIR) light irradiation, the photothermal properties of PMo12 enabled controlled low-temperature hyperthermia and accelerated BA release. Remarkably, the hydrogel achieved an antibacterial efficacy of 99.45% ± 0.12% against MRSA following 5 minutes of NIR exposure while exhibiting potent antioxidant and anti-inflammatory capabilities to scavenge reactive oxygen species and mitigate inflammatory responses. Comprehensive evaluation revealed that the PMo12-BA-PEC hydrogel significantly promoted angiogenesis, enhanced collagen deposition, inhibited bacterial growth, and modulated immune regulation, thereby accelerating the wound healing process in diabetic rat models. These findings suggest that the PMo12-BA-PEC hydrogel represents a promising biomaterial platform for clinical management of diabetic wounds and potential treatment of oxidative stress-related disorders.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3395-3408"},"PeriodicalIF":5.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porphyrin-based nanoscale metal–organic framework nanocarriers entrapping platinum nanoparticles and S-nitrosoglutathione for sonodynamic therapy in hypoxic tumors† 基于卟啉的纳米金属-有机框架纳米载体包裹纳米铂和s -亚硝基谷胱甘肽用于低氧肿瘤的声动力治疗。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-06 DOI: 10.1039/D5BM00127G

Hongbo Wang, Benchao Zheng, Shiyi Zhai, Danning Su and Kuangda Lu

{"title":"Porphyrin-based nanoscale metal–organic framework nanocarriers entrapping platinum nanoparticles and S-nitrosoglutathione for sonodynamic therapy in hypoxic tumors†","authors":"Hongbo Wang, Benchao Zheng, Shiyi Zhai, Danning Su and Kuangda Lu","doi":"10.1039/D5BM00127G","DOIUrl":"10.1039/D5BM00127G","url":null,"abstract":"Sonodynamic therapy (SDT), which employs acoustic energy to generate reactive oxygen species (ROS), has emerged as a promising strategy for tumor treatment. While ultrasound (US) offers deep tissue penetration and minimal invasiveness, the low energy conversion efficiency of sonosensitizers and the hypoxic tumor microenvironment (TME) significantly limit SDT efficacy. To overcome these challenges, we developed a nano-sonosensitizer, TBP-Hf@Pt-GSNO (Hf-Pt-G), composed of a porphyrin-based nanoscale metal–organic framework (nMOF), TBP-Hf, integrated with platinum nanoparticles (Pt NPs) and S-nitrosoglutathione (GSNO). Pt NPs within the nMOF cavities enhance ultrasound reflection and scattering, thereby improving the acoustic energy conversion efficiency of TBP and boosting SDT efficacy. In addition, Pt NPs can catalyze the conversion of endogenous hydrogen peroxide (H2O2) into oxygen to alleviate tumor hypoxia. US irradiation further triggers the release of nitric oxide (NO) from GSNO, amplifying the killing effect on tumor cells. Enhanced singlet oxygen (1O2) generation and decreased hypoxia inducible factor-1α (HIF-1α) expression were observed in tumor cells following Hf-Pt-G treatment with US irradiation. In vivo, significant tumor suppression was achieved in 4T1 tumor-bearing mice treated with Hf-Pt-G combined with US. This study presents a novel strategy for enhancing acoustic energy conversion while integrating hypoxia alleviation and controllable NO release, thus improving the therapeutic outcomes of SDT.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3325-3335"},"PeriodicalIF":5.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A bacterial cellulose-polydopamine based injectable hydrogel for enhanced hemostasis in acute wounds† 一种基于细菌纤维素-聚多巴胺的可注射水凝胶，用于加强急性伤口的止血。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2025-05-06 DOI: 10.1039/D5BM00420A

Kaushal R. Shakya, Niranjan Chatterjee, Santosh K. Misra and Vivek Verma

{"title":"A bacterial cellulose-polydopamine based injectable hydrogel for enhanced hemostasis in acute wounds†","authors":"Kaushal R. Shakya, Niranjan Chatterjee, Santosh K. Misra and Vivek Verma","doi":"10.1039/D5BM00420A","DOIUrl":"10.1039/D5BM00420A","url":null,"abstract":"An injectable hydrogel hemostat composed of bacterial cellulose (BC), polydopamine and carboxymethyl cellulose (CMC) is presented as a biocompatible alternative to generally cytotoxic commercial hemostats. In this system, polydopamine (PDA) was coated on BC fibers by in situ oxidative polymerization, and CMC was added to improve matrix injectability, as confirmed by rheological analysis showing shear thinning behavior. The composite was characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM) to assess its physical, chemical and topographical characteristics. In vitro blood clotting tests demonstrated favorable blood clotting activity, achieving hemostasis within three minutes of application. PDA's antioxidative properties additionally helped to scavenge reactive oxygen species (ROS). The composite was tested for its compatibility with blood and mammalian cells using the in vitro hemolysis assay, cell viability assay, and scratch assay. In vivo studies using rat tail amputation and liver puncture models exhibited effective hemostasis without significant toxicity. Histological analysis of skin tissue (H&E and TNF-α staining) validated the biocompatibility of the material. Thus, the BC/PDA/CMC hydrogel is a promising candidate for rapid hemostasis and wound healing, particularly in deep and irregular wounds.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3307-3324"},"PeriodicalIF":5.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0