Biomaterials Science最新文献

{"title":"Removal of expression of concern: A hypoxia-dissociable siRNA nanoplatform for synergistically enhanced chemo-radiotherapy of glioblastoma","authors":"Yandong Xie, Xueying Lu, Zhen Wang, Mingxi Liu, Liang Liu, Ran Wang, Kun Yang, Hong Xiao, Jianyong Li, Xianglong Tang and Hongyi Liu","doi":"10.1039/D5BM90042E","DOIUrl":"10.1039/D5BM90042E","url":null,"abstract":"<p >Removal of expression of concern for ‘A hypoxia-dissociable siRNA nanoplatform for synergistically enhanced chemo-radiotherapy of glioblastoma’ by Yandong Xie <em>et al.</em>, <em>Biomater. Sci.</em>, 2022, <strong>10</strong>, 6791–6803, https://doi.org/10.1039/D2BM01145J.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3409-3409"},"PeriodicalIF":5.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm90042e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering blood–brain barrier microphysiological systems to model Alzheimer's disease monocyte penetration and infiltration†","authors":"Longjun Gu, Xiangdi Mao, Chunhui Tian, Yang Yang, Kaiyuan Yang, Scott G. Canfield, Donghui Zhu, Mingxia Gu and Feng Guo","doi":"10.1039/D5BM00204D","DOIUrl":"10.1039/D5BM00204D","url":null,"abstract":"<p >Alzheimer's disease (AD) is a progressive and neurodegenerative disease, predominantly causing dementia. Despite increasing clinical evidence suggesting the involvement of peripheral immune cells such as monocytes in AD pathology, the dynamic penetration and infiltration of monocytes crossing blood–brain barrier (BBB) and inducing neuroinflammation is largely understudied in an AD brain. Herein, we engineer BBB-like microphysiological system (BBB-MPS) models for recapitulating the dynamic penetration and infiltration of monocytes in an AD patient's brain. Each BBB-MPS model can be engineered by integrating a functional BBB-like structure on a human cortical organoid using a 3D-printed device within a well of a plate. By coculturing these BBB-MPS models with monocytes from AD patients and age-matched healthy donors, we found that AD monocytes exhibit significantly greater BBB penetration and brain infiltration compared to age-matched control monocytes. Moreover, we also tested the interventions including Minocycline and Bindarit, and found they can effectively inhibit AD monocyte infiltration, subsequently reducing neuroinflammation and neuronal apoptosis. We believe these scalable and user-friendly BBB-MPS models may hold promising potential in modeling and advancing therapeutics for neurodegenerative and neuroinflammatory diseases.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 13","pages":" 3650-3661"},"PeriodicalIF":5.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and wound-healing prospect of KAPs-depleted residual hair biomaterial","authors":"Allison Meer, Aidan Mathews, Mariana Cabral, Andrew Tarabokija, Evan Carroll, Henna Chaudhry, Michelle Paszek, Nancy Radecker, Thomas Palaia, Hazel Consunji de Guzman and Roche C. de Guzman","doi":"10.1039/D4BM00777H","DOIUrl":"10.1039/D4BM00777H","url":null,"abstract":"<p >This work is an in-depth investigation of the <em>in vitro</em> and <em>in vivo</em> biocompatibility of processed and treated residual human hair samples with intact cuticle layers. The specimens included oxidized hair with minimal melanin (BLH) and hair with medium- (M-KAP) and low- (L-KAP) amounts of keratin associated proteins (KAPs), confirmed through gel electrophoresis, electron microscopy, trichrome histological staining, and tensile biomechanics, in comparison to the untreated regular hair (REG) control. All hair groups, high KAPs (H-KAPs: REG and BLH), M-KAP, and L-KAP, are non-cytotoxic in the adipose fibroblast's response to their extracts based on the ISO 10993-5 medical device biomaterial testing standard. <em>In vivo</em> mouse subcutaneous implantation (ISO 10993-6, local effects) at 2 weeks showed a foreign body response (FBR) with thin fibrous encapsulation at 28% relative skin dermis thickness; but the L-KAP implant mitigated a significant decrease in FBR area compared to H-KAPs and a lower number of immune cells of mostly macrophages and mast cells on the biomaterial's surface. In the bulk of the capsules, blood vessels and collagen extracellular matrix densities were similar among groups. These findings suggest that small globular KAPs diffuse out of the cortex to the host-biomaterial interface which induce a slightly-elevated FBR but limited to the implant's surface vicinity. For translatability, we evaluated the effectiveness of the residual hair with the most depleted KAPs (L-KAP) in a 10 mm-diameter, splinted, and full-thickness mouse skin excision wound. Treatment with the L-KAP mesh exhibited an 8% healing improvement per day compared to the untreated control: significantly reducing the projected complete healing time by 30%. On-going research focuses on purer keratin-based and macromolecularly organized residual hair biomaterials for drug-delivery as they are deemed the most biocompatible.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 13","pages":" 3633-3649"},"PeriodicalIF":5.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From saccharides to synthetics: exploring biomaterial scaffolds as cell transduction enhancers†","authors":"Micah Mallory, Emma Grace Johnson, Soumen Saha, Sanika Pandit, Joshua T. McCune, Mengnan Dennis, Jessica M. Gluck, Craig L. Duvall, Ashley C. Brown, Ashutosh Chilkoti and Yevgeny Brudno","doi":"10.1039/D4BM01588F","DOIUrl":"10.1039/D4BM01588F","url":null,"abstract":"<p >Dry, transduction biomaterial scaffolds (Drydux) represent a novel platform for enhancing viral transduction, achieving drastic improvements in transduction efficiency (from ∼10% to &gt;80%) while simplifying production of potent genetically engineered cells. This technology addresses a critical bottleneck in cell therapy manufacturing, where conventional methods require complex protocols and often yield suboptimal results. However, the underlying material science driving Drydux-enhanced transduction remains unclear. Here, we comprehensively assess biomaterial properties that influence viral transduction enhancement through systematic testing of polysaccharides, proteins, elastin-like polypeptides (ELPs), and synthetic polymers. Our findings reveal that surface porosity and liquid absorption are primary drivers of transduction enhancement, while polymer charge and flexibility play secondary roles. Negatively charged and flexible materials—particularly gelatin, hyaluronan, and alginate—demonstrated superior performance. Notably, despite promising material characteristics, synthetic polymers failed to enhance transduction, highlighting the unique advantages of specific biomaterial compositions. By elucidating these structure–function relationships, this work establishes design principles for optimizing biomaterial-enhanced transduction and expands the Drydux platform's potential for transforming cell therapy manufacturing, regenerative medicine, and beyond.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 13","pages":" 3585-3597"},"PeriodicalIF":5.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial 3D printed gelatin scaffolds for root canal disinfection in regenerative endodontics procedures","authors":"Mateo Dallos Ortega, Jenny Aveyard, Raghda Magdy Abdelgawad, Reem El-Gendy, Alexander Ciupa, David Whetnall, Julia Behnsen, Robert J. Poole and Raechelle A. D'Sa","doi":"10.1039/D5BM00440C","DOIUrl":"10.1039/D5BM00440C","url":null,"abstract":"<p >Regenerative endodontic procedures (REPs) which aim to promote root development and pulp tissue regeneration in necrotic immature teeth, have emerged as a promising therapeutic approach. A critical determinant of REP success hinges on effective disinfection of the root canal system, which must eliminate microbial contaminants whilst preserving the microenvironment necessary for dental pulp stem cell tissue regeneration. This study reports on the fabrication of biocompatible 3D printed hydrogel scaffolds designed for root canal disinfection. The scaffolds incorporate benzyldimethyldodecylammonium chloride (BDMDAC) a broad-spectrum quaternary ammonium compound characterised by low cytotoxicity and minimal risk of resistance development. BDMDAC loaded gelatin biomaterial inks were systematically evaluated for rheology properties, mechanical stability and drug release properties. Scaffolds containing 150 μg mL<small>⁻¹</small> and 250 μg mL<small>⁻¹</small> BDMDAC exhibited excellent antimicrobial efficacy against 5 bacterial pathogens (including 3 endodontic bacteria-<em>Enterococcus faecalis</em>, <em>Porphyromonas gingivalis</em>, and <em>Streptococcus mutans</em>). Cytocompatibility assays using primary human dental pulp stem cells (HDPSCs) derived from 3 donors confirmed over 70% of cell viability. Furthermore, freeze-dried scaffolds demonstrated excellent shelf-life stability for at least six months. Overall, these findings highlight the potential of 3D printed BDMDAC-loaded 3D printed gelatin scaffolds as an effective and cytocompatible platform for root canal disinfection in REPs.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 14","pages":" 3795-3813"},"PeriodicalIF":5.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00440c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress and application of cell membrane engineering in living cell therapy","authors":"Xiao-Yu Qiu, Yuan-Kai Sun, Jian-Qing Gao and Xin-Chi Jiang","doi":"10.1039/D5BM00120J","DOIUrl":"10.1039/D5BM00120J","url":null,"abstract":"<p >Cell therapy is a rapidly developing technology that has gradually made the prospect of curing various diseases become a reality. Increasing the survival rate and bioactivity of cells after transplantation is a key factor enhancing the cell therapeutic efficacy. Their targeting ability and intercellular substance communication make cells potential delivery systems. In addition, long-term and high-precision cell tracking can provide safety guarantees for cell therapy. Therefore, cell engineering has become a prominent research field to promote the application of cell therapy. The cell membrane serves as a crucial physical barrier that regulates cellular interactions with the extracellular environment, and it holds promise for contributing to the development of cell therapy. Currently, a growing number of membrane engineering techniques are being developed to customize cell surfaces and augment the therapeutic functions of cells, including direct modification, core–shell physical coating, and cellular autonomous response modification. This review comprehensively examines the latest advancements in engineering cell surfaces utilizing modification moieties to enhance the activity, targeted drug delivery capability, and labeling efficiency of transplanted cells. The distinct advantages and limitations of various membrane engineering strategies are also summarized. Here, we highlight the current challenges in this rapidly evolving field and discuss potential future opportunities.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 14","pages":" 3733-3754"},"PeriodicalIF":5.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable and self-healable supramolecular hydrogels assembled by quaternised chitosan/alginate polyelectrolyte complexation for sustained drug delivery and cell encapsulation†","authors":"Cristiana F. V. Sousa, João Borges and João F. Mano","doi":"10.1039/D5BM00072F","DOIUrl":"10.1039/D5BM00072F","url":null,"abstract":"<p >Hydrogels formed through phase separation during the complexation of oppositely charged polymers have unique properties, including fast self-assembly, hierarchical microstructures, and tunable properties. These features make them highly attractive materials for various biomedical applications, such as drug delivery, protective coatings, and surface adhesives. Notably, injectable polyelectrolyte complex (PEC) supramolecular hydrogels stand out for their minimally invasive administration and reduced trauma and side effects, providing attractive alternatives to covalent hydrogels, which are constrained by the irreversibility of their crosslinks, limiting their versatility and broader applicability. Sustainable marine-origin polysaccharides have been used for developing hydrogels due to their proven biocompatibility, non-cytotoxicity and wide bioavailability from renewable resources. In particular, chitosan (CHT) and alginate (ALG) have been widely employed to develop hydrogels, taking advantage of their opposite charge nature. However, the limited solubility of CHT under physiological conditions limits the range of bioapplications. Herein, we report the development of size- and shape-tunable PEC supramolecular hydrogels encompassing water-soluble quaternised CHT and ALG biopolymers, under physiological conditions, by polyelectrolyte complexation. The rheological and mechanical properties of the PECs are studied, demonstrating their injectability, self-healing behaviour, and cytocompatibility towards human adipose-derived stem cells. A sustained and controlled release of encapsulated fluorescein isothiocyanate-labelled bovine serum albumin is observed over fourteen days. This work paves the way for the design and development of advanced CHT-based injectable biomaterial platforms for a wide array of biomedical and biotechnological applications.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 13","pages":" 3617-3632"},"PeriodicalIF":5.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00072f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into a thermoresponsive in situ nanoemulgel of azilsartan medoxomil for intranasal delivery: a promising nanotherapeutic approach to target dementia†","authors":"Varnita Karmakar, Wei Meng Lim and Bapi Gorain","doi":"10.1039/D5BM00066A","DOIUrl":"10.1039/D5BM00066A","url":null,"abstract":"<p >Cognitive impairment and dementia have become a global burden, distressing millions of elderly and accounting for the progressive loss of neurons in the brain, affecting multiple cortical centers. The renin–angiotensin system and its receptors, widely distributed within the brain, can serve as potential targets in treating dementia by diminishing oxidative radical generation and neuronal inflammation and increasing the integrity of the blood–brain barrier. The present study delves into the formulation and optimization of a thermoresponsive azilsartan medoxomil (AZL-M)-loaded <em>in situ</em> nanoemulgel for targeted nose-to-brain delivery, addressing the challenge of restricted entry of angiotensin receptor blockers (ARBs) to the brain due to their low BBB permeability. The formulation components and mixing time for the AZL-M nanoemulsion were optimized using a Box–Behnken design approach, followed by optimization of various characterization parameters. Among the optimized gels, formulation F20 demonstrated superior characteristics for intranasal delivery, exhibiting gelation at 33.4 °C (nasal temperature), a pH of 6.21 ± 0.35, a droplet size of 160 ± 3.72 nm, a PDI of 0.21 ± 0.001, and a zeta potential of −11.2 ± 0.85 mV, with suitable viscosity at 15 °C and 35 °C. Moreover, F20 achieved 60.4% ± 5.69% cumulative drug release after 8 h, together with the highest cumulative permeation (505 ± 55.15 μg cm<small>⁻²</small>), indicating its greater efficacy in permeating nasal mucosal cells. Safety studies confirmed the biocompatibility of F20 and reduction in ROS generation with enhanced SH-SY5Y cell viability. <em>In vivo</em> studies displayed improved cognitive functions together with increased antioxidant enzyme activities (SOD, GSH, and catalase), reduced malondialdehyde levels, and enhanced neuronal count in brain histopathological studies. Furthermore, the levels of brain inflammatory markers (TNF-α and IL1-β) decreased, together with the upregulation of brain-derived neurotrophic factor (BDNF) levels, suggesting marked neuroprotection. Thus, the intranasally delivered AZL-M nanoemulgel emerges as safe and effective for treating dementia and related disorders.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 14","pages":" 3853-3875"},"PeriodicalIF":5.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro senescence and senolytic functional assays","authors":"Patrick Ryan and Jungwoo Lee","doi":"10.1039/D4BM01684J","DOIUrl":"10.1039/D4BM01684J","url":null,"abstract":"<p >A detailed understanding of aging biology and the development of anti-aging therapeutic strategies remain imperative yet inherently challenging due to the protracted nature of aging. Cellular senescence arises naturally through replicative exhaustion and is accelerated by clinical treatments or environmental stressors. The accumulation of senescent cells—defined by a loss of mitogenic potential, resistance to apoptosis, and acquisition of a pro-inflammatory secretory phenotype—has been implicated as a key driver of chronic disease, tissue degeneration, and organismal aging. Recent studies have highlighted the therapeutic promise of senolytic drugs, which selectively eliminate senescent cells. Compelling results from preclinical animal studies and ongoing clinical trials underscore this potential. However, the clinical translation of senolytics requires further pharmacological validation to refine selectivity, minimize toxicity, and determine optimal dosing. Equally important is the evaluation of senolytics’ potential to restore tissue structure and function by reducing the senescent cell burden. In vitro tissue culture models offer a powerful platform to advance these efforts. This review summarizes the current landscape of <em>in vitro</em> systems used for inducing cellular senescence—referred to as “senescence assays”—and for screening senolytic drugs—referred to as “senolytic assays”. We conclude by discussing key challenges to improving mechanistic insight, predictive accuracy, and clinical relevance in senolytic drug development, as well as emerging applications of senolytic therapies.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 13","pages":" 3509-3531"},"PeriodicalIF":5.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Dual bioresponsive antibiotic and quorum sensing inhibitor combination nanoparticles for treatment of Pseudomonas aeruginosa biofilms in vitro and ex vivo","authors":"Nishant Singh, Manuel Romero, Alessandra Travanut, Patricia F. Monteiro, Elena Jordana-Lluch, Kim R. Hardie, Paul Williams, Morgan R. Alexander and Cameron Alexander","doi":"10.1039/D5BM90038G","DOIUrl":"10.1039/D5BM90038G","url":null,"abstract":"<p >Correction for ‘Dual bioresponsive antibiotic and quorum sensing inhibitor combination nanoparticles for treatment of <em>Pseudomonas aeruginosa</em> biofilms <em>in vitro</em> and <em>ex vivo</em>’ by Nishant Singh <em>et al.</em>, <em>Biomater. Sci.</em>, 2019, <strong>7</strong>, 4099–4111, https://doi.org/10.1039/C9BM00773C.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 11","pages":" 3093-3094"},"PeriodicalIF":5.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}