Biomaterials Science最新文献_第7页

A review on hydroxyapatite fabrication: from powders to additive manufactured scaffolds 羟基磷灰石制备综述：从粉末到添加剂制备支架。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-12-18 DOI: 10.1039/D4BM00972J

Ananthika Vijayan, Jithin Vishnu, Revathi A, Balakrishnan Shankar and Sreedha Sambhudevan

{"title":"A review on hydroxyapatite fabrication: from powders to additive manufactured scaffolds","authors":"Ananthika Vijayan, Jithin Vishnu, Revathi A, Balakrishnan Shankar and Sreedha Sambhudevan","doi":"10.1039/D4BM00972J","DOIUrl":"10.1039/D4BM00972J","url":null,"abstract":"Hydroxyapatite (HA), the main inorganic bone component, is the most widely researched bioceramic for bone repair. This paper presents a comprehensive review of recent advancements in HA synthesis methods and their integration into additive manufacturing (AM) processes. Synthesis methodologies discussed include wet, dry, and biomimetic routes, emphasizing their impact on tailoring the physicochemical properties of HA for biomedical applications. The incorporation of dopants and additives during synthesis is explored for optimizing the mechanical, biological, and osteogenic characteristics of HA-based materials. Moreover, the evolution of AM technologies from conventional 3D printing to advanced 4D and 5D printing is detailed, covering material selection, process parameters, and post-processing strategies vital for fabricating intricate, patient-specific scaffolds, implants, and drug delivery systems utilizing HA. The review underscores the importance of achieving precise control over microstructure and porosity to mimic native tissue architectures accurately. Furthermore, emerging applications of HA-based constructs in tissue engineering, regenerative medicine, drug delivery, and orthopedic implants are discussed, highlighting their potential to address critical clinical needs. Despite the glimmer of hope provided by the advent and progress of such AM capabilities, several aspects need to be addressed to develop efficient HA-based bone substitutes, which are explored in detail in this review.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 4","pages":" 913-945"},"PeriodicalIF":5.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulate PD-L1's membrane orientation thermodynamics with hydrophobic nanoparticles† 疏水纳米颗粒调节PD-L1的膜取向热力学。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-12-18 DOI: 10.1039/D4BM01469C

Xiaoqian Lin and Xubo Lin

{"title":"Regulate PD-L1's membrane orientation thermodynamics with hydrophobic nanoparticles†","authors":"Xiaoqian Lin and Xubo Lin","doi":"10.1039/D4BM01469C","DOIUrl":"10.1039/D4BM01469C","url":null,"abstract":"Tumor cells can escape from immune killing by binding their programmed death ligand-1 (PD-L1) to the programmed cell death protein 1 (PD-1) of T cells. These immune checkpoint proteins (PD-L1/PD-1) have become very important drug targets, since blocking PD-L1 or PD-1 can recover the killing capability of T cells against tumor cells. Instead of targeting the binding interface between PD-L1 and PD-1, we explored the possibility of regulating the membrane orientation thermodynamics of PD-L1 with ligand-modified ultra-small hydrophobic nanoparticles (NPs) using μs-scale coarse-grained molecular dynamics (MD) simulations in this work. Our MD results indicate that embedded hydrophobic NPs can significantly change the membrane orientation thermodynamics of the extracellular domain of PD-L1, enhancing the probability in the “stand up” state for better binding to PD-1. Meanwhile, embedded hydrophobic NPs promote the tilt of the transmembrane domain of PD-L1. Besides, effects on both extracellular and transmembrane domains are determined by the ligand length and NP concentration. Our study may provide an alternative strategy to achieve PD-L1-related immunotherapy with nanomedicine.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 3","pages":" 826-835"},"PeriodicalIF":5.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pericardial bioscaffold coated with ECM gels and urothelial cells for the repair of a rabbit urinary bladder defect. 包被ECM凝胶和尿路上皮细胞的心包生物支架修复兔膀胱缺损。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-12-17 DOI: 10.1039/d4bm00846d

Guadalupe H Luevano-Colmenero, Rosalinda Rocha-Juache, Juan Vargas-Mancilla, Jorge M Flores-Moreno, Francisco J Rojo, Gustavo V Guinea, Birzabith Mendoza-Novelo

{"title":"Pericardial bioscaffold coated with ECM gels and urothelial cells for the repair of a rabbit urinary bladder defect.","authors":"Guadalupe H Luevano-Colmenero, Rosalinda Rocha-Juache, Juan Vargas-Mancilla, Jorge M Flores-Moreno, Francisco J Rojo, Gustavo V Guinea, Birzabith Mendoza-Novelo","doi":"10.1039/d4bm00846d","DOIUrl":"https://doi.org/10.1039/d4bm00846d","url":null,"abstract":"Repair of damaged or faulty complex modular organs such as the urinary bladder is a current clinical challenge. The design of constructs for reconstructive urological surgery can draw advantage from the bioactivity of natural extracellular matrix (ECM) bioscaffolds, as well as the activity provided by cells seeded into constructs. Considering these benefits, this work compares the performance of pericardial ECM bioscaffolds and constructs seeded with gel-supported urothelial cells in the repair of urinary bladder defects in rabbits. The bioscaffolds considered in this study are of porcine (pM) and bovine (bM) origin and exhibited a residual composition that confers bioactivity in mesh presentation. Coating an ECM gel on the bioscaffolds promoted the adhesion and viability of urothelial cells. Repairing a full-thickness urinary bladder defect in a rabbit model with the bioscaffolds and constructs resulted in the integration with the host bladder; meanwhile, bladder volumetric capacity was promoted using bM and constructs. Although no contribution of gel/cell seeding to the failure of mechanical properties of the urinary neobladder was observed, this seeding technique is suitable for integration with different strategies to engineer constructs for urinary bladder reconstructive surgery.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Permeation enhancer decorated nanoparticles for oral delivery of insulin: manipulating the surface density of borneol and PEG for absorption barriers† 用于口服胰岛素递送的渗透增强剂修饰纳米颗粒：操纵冰片和聚乙二醇的表面密度作为吸收屏障。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-12-17 DOI: 10.1039/D4BM01210K

Xiaoyu Yang, Jidong Zhang, Jitang Chen, Yunxuan Xie, Tianci Hu, Qin Luo, Tianhao Peng, Han Luo, Linlin Shi, Jiangling Wan, Jianxin Wang, Xiangliang Yang and Jianyong Sheng

{"title":"Permeation enhancer decorated nanoparticles for oral delivery of insulin: manipulating the surface density of borneol and PEG for absorption barriers†","authors":"Xiaoyu Yang, Jidong Zhang, Jitang Chen, Yunxuan Xie, Tianci Hu, Qin Luo, Tianhao Peng, Han Luo, Linlin Shi, Jiangling Wan, Jianxin Wang, Xiangliang Yang and Jianyong Sheng","doi":"10.1039/D4BM01210K","DOIUrl":"10.1039/D4BM01210K","url":null,"abstract":"Oral protein drugs’ delivery faces challenges due to multiple absorption barriers for macromolecules. Co-administration with permeation enhancers and encapsulation in nano-carriers are two promising strategies to enhance their oral absorption. Herein, the poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) are decorated with polyethylene glycol (PEG) and a traditional Chinese medicine-derived permeation enhancer borneol (BO) for oral insulin delivery. Compared with a physical mixture of BO and PEG-decorated PLGA NPs, PLGA–PEG–BO NPs significantly facilitate insulin permeation across intestinal epithelia through various transcytosis pathways. The relationship among the BO surface density, physico-chemical properties and multiple barriers penetration ability is further investigated. Increasing the BO density boosts penetration through the epithelial cell layer but reduces enzyme and mucus barrier penetration. When the surface PEG density is at 90% and BO density is at 10%, the NPs possess the strongest overall ability to overcome both the mucus layer barrier and epithelial cell barrier, as illustrated by the highest permeation efficiency through Caco-2/HT29-MTX cell co-cultural monolayers. In diabetic rodents, PLGA–PEG90%–BO10% NPs exhibit high intestinal safety and a substantial hypoglycemic effect, with insulin availability at 6.22 ± 2.30%, double that of orally delivered insulin PLGA–PEG NPs and far superior to a physical mixture with BO. This study reveals the importance of tailored absorption enhancer decoration for oral protein delivery.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 3","pages":" 743-757"},"PeriodicalIF":5.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Fabrication of yeast β-glucan/sodium alginate/γ-polyglutamic acid composite particles for hemostasis and wound healing 更正：酵母β-葡聚糖/海藻酸钠/γ-聚谷氨酸复合颗粒用于止血和伤口愈合。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-12-17 DOI: 10.1039/D4BM90099E

Qinglin Zou, Hongdong Duan, Shimin Fang, Wenlong Sheng, Xiaobin Li, Rostyslav Stoika, Nataliya Finiuk, Rostyslav Panchuk, Kechun Liu and Lizhen Wang

引用次数: 0

Correction: Hybrid cell membranes camouflage liposomes containing payloads to improve breast cancer chemo and photodynamic therapy 更正：混合细胞膜伪装含有有效载荷的脂质体，改善乳腺癌化疗和光动力疗法。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-12-16 DOI: 10.1039/D4BM90100B

Chengfang Wang and Size Wu

引用次数: 0

Bio-inspired anti-swelling amyloid-fiber lysozyme adhesive for rapid wound closure and hemostasis 仿生抗肿胀淀粉纤维溶菌酶胶粘剂，用于快速伤口愈合和止血。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-12-12 DOI: 10.1039/D4BM01494D

Tingwu Liu, Zilin Wang, Xu Zhang, Donghua Xu, Qiuyan Yan, Yuanwei Chen and Shifang Luan

{"title":"Bio-inspired anti-swelling amyloid-fiber lysozyme adhesive for rapid wound closure and hemostasis","authors":"Tingwu Liu, Zilin Wang, Xu Zhang, Donghua Xu, Qiuyan Yan, Yuanwei Chen and Shifang Luan","doi":"10.1039/D4BM01494D","DOIUrl":"10.1039/D4BM01494D","url":null,"abstract":"Instant adhesion to wet biological surfaces and reduced swelling of tissue adhesives are crucial for rapid wound closure and hemostasis. However, previous strategies to reduce swelling were always accompanied by a decrease in the tissue bonding strength of the adhesive. Moreover, the irreducibility of the covalent bonds in currently reported adhesives results in the adhesives losing their tissue adhesive ability. To tackle the challenge, a superior anti-swelling coacervate adhesive possessing fast self-healing properties through physical interactions (electrostatic interactions, hydrogen bonding) and chemical crosslinking (Schiff base reaction) was obtained with aldehyde-modified γ-PGA (γ-PGA-CHO), a natural lysozyme (LZM) and an amyloid fiber reduced lysozyme (RLZM). The instant shear adhesion strength and burst pressure tolerance of the adhesive on wet pig intestine reached 50.8 kPa (2.6 times that of CA glue) and 142.5 mmHg (5.9 times that of CA glue), and it maintained an adhesion strength of 37.4 kPa after exposure to the physical environment for 12 h and the swelling rate was only 34.0% underwater. The in vitro and in vivo experiments provided the coacervate adhesive with potential applicability for emergency rescue and wound care scenarios.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 3","pages":" 688-696"},"PeriodicalIF":5.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anisotropic conductive scaffolds for post-infarction cardiac repair 各向异性导电支架在梗死后心脏修复中的应用。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-12-11 DOI: 10.1039/D4BM01109K

Shimin Li, Wenming Yin, Yali Liu, Chang Yang, Zitong Zhai, Mingxiang Xie, Ziyi Ye and Xiaoping Song

{"title":"Anisotropic conductive scaffolds for post-infarction cardiac repair","authors":"Shimin Li, Wenming Yin, Yali Liu, Chang Yang, Zitong Zhai, Mingxiang Xie, Ziyi Ye and Xiaoping Song","doi":"10.1039/D4BM01109K","DOIUrl":"10.1039/D4BM01109K","url":null,"abstract":"Myocardial infarction (MI) remains one of the most common and lethal cardiovascular diseases (CVDs), leading to the deterioration of cardiac function due to myocardial cell necrosis and fibrous scar tissue formation. Myocardial infarction (MI) remains one of the most common and lethal cardiovascular diseases (CVDs), leading to the deterioration of cardiac function due to myocardial cell necrosis and fibrous scar tissue formation. After MI, the anisotropic structural properties of myocardial tissue are destroyed, and its mechanical and electrical microenvironment also undergoes a series of pathological changes, such as ventricular wall stiffness, abnormal contraction, conduction network disruption, and irregular electrical signal propagation, which may further induce myocardial remodeling and even lead to heart failure. Therefore, bionic reconstruction of the anisotropic structural–mechanical–electrical microenvironment of the infarct area is key to repairing damaged myocardium. This article first summarizes the pathological changes in muscle fibre structure and conductive microenvironment after cardiac injury, and focuses on the classification and preparation methods of anisotropic conductive materials. In addition, the effects of these anisotropic conductive materials on the behavior of cardiac resident cells after myocardial infarction, such as directional growth, maturation, proliferation and migration, and the differentiation fate of stem cells and the possible molecular mechanisms involved are summarized. The design strategies for anisotropic conductive scaffolds for myocardial repair in future clinical research are also discussed, with the aim of providing new insights for researchers in related fields.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 3","pages":" 542-567"},"PeriodicalIF":5.8,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anionic polymer coating for enhanced delivery of Cas9 mRNA and sgRNA nanoplexes† 阴离子聚合物涂层增强Cas9 mRNA和sgRNA纳米复合物的递送。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-12-11 DOI: 10.1039/D4BM01290A

Siyu Chen, Simone Pinto Carneiro and Olivia M. Merkel

{"title":"Anionic polymer coating for enhanced delivery of Cas9 mRNA and sgRNA nanoplexes†","authors":"Siyu Chen, Simone Pinto Carneiro and Olivia M. Merkel","doi":"10.1039/D4BM01290A","DOIUrl":"10.1039/D4BM01290A","url":null,"abstract":"Polymeric carriers have long been recognized as some of the most effective and promising systems for nucleic acid delivery. In this study, we utilized an anionic di-block co-polymer, PEG-PLE, to enhance the performance of lipid-modified PEI (C14-PEI) nanoplexes for delivering Cas9 mRNA and sgRNA targeting KRAS G12S mutations in lung cancer cells. Our results demonstrated that PEG-PLE, when combined with C14-PEI at a weight-to-weight ratio of 0.2, produced nanoplexes with a size of approximately 140 nm, a polydispersity index (PDI) of 0.08, and a zeta potential of around −1 mV. The PEG-PLE/C14-PEI nanoplexes at this ratio were observed to be both non-cytotoxic and effective in encapsulating Cas9 mRNA and sgRNA. Confocal microscopy imaging revealed efficient endosomal escape and intracellular distribution of the RNAs. Uptake pathway inhibition studies indicated that the internalization of PEG-PLE/C14-PEI primarily involves scavenger receptors and clathrin-mediated endocytosis. Compared to C14-PEI formulations, PEG-PLE/C14-PEI demonstrated a significant increase in luciferase mRNA expression and gene editing efficiency, as confirmed by T7EI and ddPCR, in A549 cells. Sanger sequencing identified insertions and/or deletions around the PAM sequence, with a total of 69% indels observed. Post-transfection, the KRAS-ERK pathway was downregulated, resulting in significant increases in cell apoptosis and inhibition of cell migration. Taken together, this study reveals a new and promising formulation for CRISPR delivery as potential lung cancer treatment.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 3","pages":" 659-676"},"PeriodicalIF":5.8,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11650648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applications and prospects of indirect 3D printing technology in bone tissue engineering 间接3D打印技术在骨组织工程中的应用与展望。

IF 5.8 3区医学

Biomaterials Science Pub Date : 2024-12-09 DOI: 10.1039/D4BM01374C

Mingxin Qiao, Weimin Wu, Wen Tang, Yifan Zhao, Jian Wang, Xibo Pei, Bowen Zhang and Qianbing Wan

{"title":"Applications and prospects of indirect 3D printing technology in bone tissue engineering","authors":"Mingxin Qiao, Weimin Wu, Wen Tang, Yifan Zhao, Jian Wang, Xibo Pei, Bowen Zhang and Qianbing Wan","doi":"10.1039/D4BM01374C","DOIUrl":"10.1039/D4BM01374C","url":null,"abstract":"In bone tissue engineering, manufacturing bone tissue constructs that closely replicate physiological features for regenerative repair remains a significant challenge. In recent years, the advent of indirect 3D printing technology has overcome the stringent material demands, confined resolution, and structural control challenges inherent to direct 3D printing. By utilizing sacrificial templates, the natural structures and physiological functions of bone tissues can be precisely duplicated. It facilitates the fabrication of vascularized and biomimetic bone constructs that are similar to natural counterparts. Hence, indirect 3D printing technology is increasingly recognized as a promising option for bone regenerative therapies. Based on the aforementioned research hotspots, this review outlines the classification and techniques of indirect 3D printing, along with the associated printing materials and methodologies. More importantly, a detailed summary of the clinical application prospects of indirect 3D printing in the regeneration of bone, cartilage and osteochondral tissues is provided, along with exploring the current challenges and outlook of this technology.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 3","pages":" 587-605"},"PeriodicalIF":5.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0