Biomaterials Science最新文献

{"title":"Machine-learning-guided identification of protein secondary structures using spectral and structural descriptors†","authors":"Ziqi Wang and Kenry","doi":"10.1039/D5BM00153F","DOIUrl":"10.1039/D5BM00153F","url":null,"abstract":"<p >Interrogation of the secondary structures of proteins is essential for designing and engineering more effective and safer protein-based biomaterials and other classes of theranostic materials. Protein secondary structures are commonly assessed using circular dichroism spectroscopy, followed by relevant downstream analysis using specialized software. As many proteins have complex secondary structures beyond the typical α-helix and β-sheet configurations, and the derived secondary structural contents are significantly influenced by the selection of software, estimations acquired through conventional methods may be less reliable. Herein, we propose the implementation of a machine-learning-based approach to improve the accuracy and reliability of the classification of protein secondary structures. Specifically, we leverage supervised machine learning to analyze the circular dichroism spectra and relevant attributes of 112 proteins to predict their secondary structures. Based on a range of spectral, structural, and molecular features, we systematically evaluate the predictive performance of numerous supervised classifiers and identify optimal combinations of algorithms with descriptors to achieve highly accurate and precise estimations of protein secondary structures. We anticipate that this work will offer a deeper insight into the development of machine-learning-based approaches to streamline the delineation of protein structures for different biological and biomedical applications.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 11","pages":" 2973-2982"},"PeriodicalIF":5.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00153f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of laser-induced graphene for skeletal muscle tissue engineering applications","authors":"Jesús Ordoño, Monsur Islam, Andrés Díaz Lantada, Mónica Echeverry-Rendón and De-Yi Wang","doi":"10.1039/D5BM00174A","DOIUrl":"10.1039/D5BM00174A","url":null,"abstract":"<p >This study explores the potential of laser-induced graphene (LIG) as a conductive, biocompatible material for musculoskeletal tissue engineering applications. Using laser irradiation, polyimide (PI) substrates were transformed into highly graphitic, porous LIG with a distinct fibril morphology and surface topography. Characterization analyses, including Raman spectroscopy and X-ray diffraction (XRD), confirmed the graphitic nature of LIG, while electrical conductivity measurements indicated a value of 5.8 ± 0.2 S cm<small>⁻¹</small>, with the surface demonstrating hydrophobicity (contact angle of 95.3° ± 1.9°). Biocompatibility tests using the C2C12 myoblast cell line showed high cell viability and alignment along the laser-induced pattern of LIG, an attribute essential for muscle tissue engineering. Cells cultured on LIG demonstrated progressive proliferation and expression of myogenic markers under reduced serum conditions, indicating the ability of LIG to support myogenic differentiation. These findings highlight LIG as a promising biomaterial that combines bioelectrical functionality with structural support, offering new avenues for developing advanced microsystems interacting with cells, leading to novel tissue engineering solutions for muscle repair and regeneration.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3242-3251"},"PeriodicalIF":5.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulus-responsive hydrogels for diabetic wound management via microenvironment modulation","authors":"Yang Gao, Xinxin Chen, Chaoliang He, Zhen Zhang and Jiaao Yu","doi":"10.1039/D4BM01657B","DOIUrl":"10.1039/D4BM01657B","url":null,"abstract":"<p >Diabetic wounds, a major complication of diabetes mellitus, pose a significant clinical challenge. The treatment of diabetic wounds requires comprehensive interventions tailored to their pathophysiological characteristics, such as recurring bacterial infection, persistent inflammation, excessive oxidative stress, and impaired angiogenesis. The development of stimulus-responsive hydrogel dressings offers new strategies for diabetic wound treatment. By responding to various physical and biochemical signals, these smart hydrogels enable real-time monitoring and precise modulation of the wound microenvironment to accelerate diabetic wound healing. In this review, we provide an overview of the disease characteristics of chronic diabetic wounds and introduce the current clinical treatment approaches. We summarize the cutting-edge applications of physical and biochemical signal-responsive hydrogels for diabetic wound treatment by modulating the wound microenvironment.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3192-3212"},"PeriodicalIF":5.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guiding vascular infiltration through architected GelMA/PEGDA hydrogels: an in vivo study of channel diameter, length, and complexity†","authors":"Martha Fowler, Alvaro Moreno Lozano, Julian Krause, Patrick Bednarz, Shalini Pandey, Mina Ghayour, Qixu Zhang and Omid Veiseh","doi":"10.1039/D5BM00193E","DOIUrl":"10.1039/D5BM00193E","url":null,"abstract":"<p >Organ shortages for transplantation in the United States impact over 100 000 patients, with 17 dying daily due to the lack of available organs. This growing need is exacerbated by the limited functionality and disease risk of donated organs. Tissue-engineered organs present a promising alternative, requiring optimized scaffold architecture and cell integration. Vascular networks within organs are essential for supplying oxygen and nutrients to cells, with a critical distance between blood vessels and surrounding tissue to allow effective diffusion. Various microfabrication techniques, such as electrospinning, freeze-drying, and gas foaming, have been employed to develop engineered organs. However, these techniques often lack the complexity needed to support vascularization. 3D bioprinting, particularly digital light projection (DLP)-based stereolithography, offers a solution by enabling high-resolution control of both external and internal architectures. Gelatin methacrylate (GelMA) and polyethylene glycol diacrylate (PEGDA) hydrogels have shown potential for tissue integration in simple structures but require further optimization for vascularization in more complex constructs. This study utilizes DLP to 3D bioprint GelMA/PEGDA hydrogels, exploring various channel designs to enhance tissue infiltration and vascularization in rodent models, providing a potential platform for cell and tissue transplantation. We demonstrate that GelMA/PEGDA hydrogels are mechanically robust, biocompatible, and support <em>in vivo</em> vascular infiltration. Channel diameter significantly influenced vascularization, with 1 mm channels yielding the highest infiltration, while channel length had minimal impact. Among five tested architectures, one design (GEO3) promoted the greatest vascular ingrowth, establishing a tunable hydrogel platform for prevascularized tissue engineering applications.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 11","pages":" 2951-2960"},"PeriodicalIF":5.8,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00193e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteoimmunomodulation by bone implant materials: harnessing physicochemical properties and chemical composition","authors":"Mehdi Sanati, Ines Pieterman, Natacha Levy, Tayebeh Akbari, Mohamadreza Tavakoli, Alireza Hassani Najafabadi and Saber Amin Yavari","doi":"10.1039/D5BM00357A","DOIUrl":"10.1039/D5BM00357A","url":null,"abstract":"<p >Chronic inflammation at bone defect sites can impede regenerative processes, but local immune responses can be adjusted to promote healing. Regulating the osteoimmune microenvironment, particularly through macrophage polarization, has become a key focus in bone regeneration research. While bone implants are crucial for addressing significant bone defects, they are often recognized by the immune system as foreign, triggering inflammation that leads to bone resorption and implant issues like fibrous encapsulation and aseptic loosening. Developing osteoimmunomodulatory implants offers a promising approach to transforming destructive inflammation into healing processes, enhancing implant integration and bone regeneration. This review explores strategies based on tuning the physicochemical attributes and chemical composition of materials in engineering osteoimmunomodulatory and pro-regenerative bone implants.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 11","pages":" 2836-2870"},"PeriodicalIF":5.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00357a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-viral mRNA delivery to the lungs","authors":"Lauren Healy, Breanna Y. Seto, Haissi Cui and Bowen Li","doi":"10.1039/D5BM00322A","DOIUrl":"10.1039/D5BM00322A","url":null,"abstract":"<p >The rapid advancement of mRNA therapeutics, exemplified by COVID-19 vaccines, underscores the transformative potential of non-viral delivery systems. However, achieving efficient and targeted mRNA delivery to the lungs remains a critical challenge due to biological barriers such as pulmonary mucus, nanoparticle instability, and off-target accumulation particularly in the liver. Addressing these challenges is crucial for advancing treatments for respiratory diseases, including cystic fibrosis, primary ciliary dyskinesia, and lung cancers. This review highlights emerging strategies to enhance lung-targeted mRNA delivery, focusing on lipid nanoparticles, polymeric nanoparticles, lipid–polymer hybrids, and peptide/protein conjugates. By discussing advances in bioinspired design and nanoparticle reformulation, this review provides a roadmap for overcoming current delivery limitations and accelerating the clinical translation of lung-targeted mRNA therapies.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 11","pages":" 2871-2882"},"PeriodicalIF":5.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00322a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of a quinoxalinone-based AIE probe for the detection of ROS in in vitro and in vivo sepsis models†","authors":"Jing Ma, Xinyu Zhang, Junlong Xiong, Che Zhang, Shimao Zhu, Weiling Cao, Jie Wei and Peng Zhang","doi":"10.1039/D5BM00352K","DOIUrl":"10.1039/D5BM00352K","url":null,"abstract":"<p >Sepsis is one of the major causes of long-term mortality; the identification of potential biomarkers and developing specific and sensitive imaging and detection methods are crucial for timely diagnosis and progression evaluation. Reactive oxygen species (ROS) may serve as a potential detection and imaging marker for sepsis. Herein, we designed and synthesized a near-infrared quinoxalone framework-based aggregation-induced emission probe (<strong>QuinoNS</strong> NPs). We evaluated the selectivity, cytotoxicity, and detection and imaging ability in an <em>in vitro</em> LPS induced inflammatory model and an <em>in vivo</em> sepsis model. The probe can respond to ROS, causing a blue shift in the fluorescence emission wavelength. The probe can achieve real-time imaging and detection of ROS in LPS induced sepsis models both <em>in vitro</em> and <em>in vivo</em> with quick response and a superior duration time without significant toxicity. This study provides new strategies and theoretical basis for imaging and diagnosis of inflammatory diseases such as sepsis.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3298-3306"},"PeriodicalIF":5.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fe3O4/MnCO3 microbubbles for efficient elimination of bacterial biofilms by mechanical/sonodynamic effects under ultrasound irradiation and magnetic field targeting†","authors":"Lihui Yuwen, Yuan Liu, Fengjiao Xu, Chi Zhang, Xiaolong Chen, Zhaowei Yin, Bin Liang and Lianhui Wang","doi":"10.1039/D5BM00227C","DOIUrl":"10.1039/D5BM00227C","url":null,"abstract":"<p >Bacterial biofilms present significant challenges in treatment with traditional antibiotics. Ultrasound (US)-responsive antibacterial agents have emerged as promising alternatives for treating bacterial biofilm infections. However, these agents are often limited by antibiotic dependence, inadequate targeting, and low antibacterial efficacy. Herein, we develop Fe<small>₃</small>O<small>₄</small>/MnCO<small>₃</small> microbubbles (FMMB) by self-assembly of Fe<small>₃</small>O<small>₄</small> nanoparticles (Fe<small>₃</small>O<small>₄</small> NPs) and MnCO<small>₃</small> nanoparticles (MnCO<small>₃</small> NPs). Under the direction of the magnetic field (MF), FMMB can be directed toward the methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) biofilm. Under US irradiation, FMMB can disrupt the structure of MRSA biofilms by cavitation-induced mechanical effects and kill bacteria with reactive oxygen species (ROS) generated by MnCO<small>₃</small> NPs through the sonodynamic effect. In a mouse model with catheter-associated MRSA biofilm infection, FMMB removed 58.8% of the biofilm with MF and US, and the bacterial inactivation efficiency reached as high as 4.1 log (99.992%). This work develops multifunctional microbubbles with both US-responsive mechanical and sonodynamic effects for biofilm disruption and MF-responsive properties for biofilm targeting, offering a promising strategy for designing antibiofilm agents to effectively treat bacterial biofilm infections.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3367-3379"},"PeriodicalIF":5.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimeric interacting interface of biologically synthesized zinc oxide nanoparticle corona efficiently sequesters α-synuclein against protein fibrillation†","authors":"Sonali Jena, Kumari Subham, Harshit Kalra and Suman Jha","doi":"10.1039/D5BM00143A","DOIUrl":"10.1039/D5BM00143A","url":null,"abstract":"<p >Parkinson's disease (PD) is characterized by the loss of dopaminergic neurons along with the accumulation of amyloid plaques with alpha-synuclein (αS) as the major constituent. αS is an intrinsically disordered protein with the potential to undergo a cascade of structural transitions from a soluble disordered conformation to ordered cross-β-sheet-rich insoluble amyloid fibrils. Small molecules like polyphenols and peptides with anti-amyloidogenic potential can mitigate fibrillation <em>in vitro</em> but fail <em>in vivo</em> owing to poor bioavailability. To overcome this problem, a platform that simultaneously enhances the bioavailability of the mitigators and efficiently sequesters αS monomers against amyloidosis is needed. Accordingly, herein, the sequestering potential of surface-moderated zinc oxide nanoparticles was explored; <em>in silico</em> and <em>in vitro</em> studies showed that the moderated nano-interfaces efficiently sequestered αS in amorphous aggregates, which were termed as flocs. Moreover, GC-MS-based analysis of the bio-nano corona highlighted the rationale for efficient sequestering of αS monomers against amyloidosis by the biologically synthesized zinc oxide nanoparticle compared with other nanoparticle surfaces. Thus, this work exemplifies the multimeric interacting interface as a platform to efficiently sequester the αS protein and simultaneously enhance the bioavailability of the phytochemicals.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3336-3353"},"PeriodicalIF":5.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chondrogenic and chondroprotective response of composite collagen I/II-hyaluronic acid scaffolds within an inflammatory osteoarthritic environment†","authors":"Carly M. Battistoni, Javier Munoz Briones, Douglas K. Brubaker, Alyssa Panitch and Julie C. Liu","doi":"10.1039/D5BM00033E","DOIUrl":"10.1039/D5BM00033E","url":null,"abstract":"<p >Inflammation plays a key role in cartilage damage that occurs in osteoarthritis (OA). However, <em>in vitro</em> assessments of tissue-engineered constructs for cartilage regeneration generally do not consider their performance in the presence of inflammation. In this work, the chondrogenic differentiation potential of mesenchymal stromal cells (MSCs) was evaluated in the presence of both chondrogenic factors and inflammatory cytokines, and cartilage formation, degradative response, and inflammatory response were characterized. The addition of cytokines reduced cartilage production, increased cell proliferation, and resulted in an increase in inflammatory markers. Incorporation of hyaluronic acid (HA) had little impact on both collagen fibril microstructure and mechanical properties, two gel properties known to affect cell response, and thus allows the work to probe the biological impact of HA without the confounding effect of these gel properties. Regardless of <em>in vitro</em> environment, HA did not change cartilage production. The inflammatory response was similar with or without HA in terms of IL-6 and IL-10 secretion whereas IL-8 production exhibited some correlation with HA concentration as observed <em>via</em> a linear regression model. Additionally, in the presence of cytokines, inclusion of HA statistically decreased the gene- and protein-level expression of matrix metalloproteinase-13 (MMP-13). Thus, when exposed to both chondrogenic growth factors and inflammatory cytokines within a chondrogenic-promoting collagen I/II blended hydrogel, chondrogenic differentiation of MSCs was limited by the inflammatory environment. These findings emphasize the importance of understanding how biomaterials affect cell responses within disease-relevant inflammatory environments.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 12","pages":" 3252-3263"},"PeriodicalIF":5.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00033e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}