Biomaterials Science最新文献

{"title":"Antimicrobial and anti-inflammatory effects of polyethyleneimine-modified polydopamine nanoparticles on a burn-injured skin model†","authors":"Sadman Sakib, Nesha May O. Andoy, Jessica Y. C. Yang, Anna Galang, Ruby May A. Sullan and Shan Zou","doi":"10.1039/D4BM01530D","DOIUrl":"10.1039/D4BM01530D","url":null,"abstract":"<p >Chronic infections involving bacterial biofilms pose significant treatment challenges due to the resilience of biofilms against existing antimicrobials. Here, we introduce a nanomaterial-based platform for treating <em>Staphylococcus epidermidis</em> biofilms, both in isolation and within a biofilm-infected burn skin model. Our approach leverages biocompatible and photothermal polydopamine nanoparticles (PDNP), functionalized with branched polyethyleneimine (PEI) and loaded with the antibiotic rifampicin, to target bacteria dwelling within biofilms. A key innovation of our method is its ability to not only target planktonic <em>S. epidermidis</em> but also effectively tackle biofilm-embedded bacteria. We demonstrated that PDNP–PEI interacts effectively with the bacterial surface, facilitating laser-activated photothermal eradication of planktonic <em>S. epidermidis</em>. In a 3D skin burn injury model, PDNP–PEI demonstrates anti-inflammatory and reactive oxygen species (ROS)-scavenging effects, reducing inflammatory cytokine levels and promoting healing. The rifampicin-loaded PDNP–PEI (PDNP–PEI–Rif) platform further shows significant efficacy against bacteria inside biofilms. The PDNP–PEI–Rif retained its immunomodulatory activity and efficiently eradicated biofilms grown on our burn-injured 3D skin model, effectively addressing the challenges of biofilm-related infections. This achievement marks a significant advancement in infection management, with the potential for a transformative impact on clinical practice.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 7","pages":" 1770-1783"},"PeriodicalIF":5.8,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d4bm01530d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Hot-dog-string” drug-eluting degradable stents for treating stenosis in tortuous arteries","authors":"Chen-Hung Lee, Pin-Chao Feng, Shih-Jie Hsu, Yi-Hua Kuo and Shih-Jung Liu","doi":"10.1039/D4BM01478B","DOIUrl":"10.1039/D4BM01478B","url":null,"abstract":"<p >Despite advances in cardiovascular technology, treating stenosis in tortuous arteries with balloon-expandable stents, typically deployed in a straight orientation, remains a challenge. This study developed novel balloon-expandable “hot-dog-string” (HDS) drug-eluting poly(ε-caprolactone) (PCL) nanofibrous stents using solvent casting and coaxial electrospinning techniques. A unique HDS geometry was designed for the PCL stent backbone, while aspirin and sirolimus were loaded into the core–sheath structured poly(lactic-<em>co</em>-glycolic acid) (PLGA) nanofibers, which were then wrapped around the degradable stent. <em>In vitro</em> characterization of the biodegradable HDS stent and drug-eluting nanofibers was conducted. The results indicate that the biodegradable HDS stents exhibited excellent mechanical properties and superior flexibility, allowing them to navigate curved sections of a simulated <em>in vitro</em> vessel model more effectively than metallic stents. The core–sheath structure of the nanofibers enabled sustained release of high concentrations of aspirin and sirolimus over 14 and 23 days, respectively, with sirolimus effectively inhibiting smooth muscle cell proliferation. Moreover, <em>in vivo</em> animal tests showed that the rabbits remained in good health with excellent vessel patency following stent placement. By implementing the innovative design and techniques proposed in this study, we anticipate fabricating biodegradable drug-eluting HDS stents of various sizes for diverse cardiovascular applications at curved lesions.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 7","pages":" 1758-1769"},"PeriodicalIF":5.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Titanium implants trigger extra-periodontal T cell-mediated immunity†","authors":"Li-Tzu Wang, Sin-Ei Juang, Chi-Lun Lan, Hsuan-Hao Chang, Ai-Chia He, Wei-An Chen, Yu-Wen Huang, Thomas E. Van Dyke, Yi-Wen Chen and Kevin Sheng-Kai Ma","doi":"10.1039/D4BM00246F","DOIUrl":"10.1039/D4BM00246F","url":null,"abstract":"<p >Peri-implant inflammation and periodontitis share a common etiology rooted in periodontopathic bacterial invasion, with periodontitis notably linked to systemic inflammatory comorbidities involving T cells. However, the intricate processes within the peri-implant microenvironment and systemic repercussions of implants, particularly related to implant materials, remain inadequately understood. We aim to elucidate the impact of contact with titanium materials, widely employed in dental implants for their high biocompatibility and excellent corrosion resistance, on diverse T cell subpopulations. This study adopts a comprehensive approach, encompassing (1) transcriptomic profiling of peri-implant epithelium in a rat model, (2) examination of phenotypic and functional changes in T cell immunity in human blood cells cultured on titanium discs, and (3) <em>in vivo</em> validation of T cells in implanted mice. Transcriptomic evidence and functional <em>in vitro</em> results revealed that exposure to titanium materials promoted T cell activation and differentiation towards inflammatory subsets, and escalated the secretion of corresponding cytokines. <em>In vivo</em> results showed that most of the gingiva-extracted T cells were activated in both healthy and implanted mice, the latter exhibiting significant lymphadenitis. High-dimensional flow cytometric findings in the <em>in vivo</em> lymphadenitis model indicated titanium-induced T cell immunity, involving preferential activation of Th1, Th17, and Tc1 cells over Tregs in adjacent lymph nodes within three days after implant placement. These findings highlight the pivotal role of T cells in the initiation of peri-implant inflammation, emphasizing the need to understand extra-periodontal inflammatory complications associated with implant surgeries. Our study provides a foundation for future therapeutic strategies targeting T cell responses to enhance the success and longevity of dental implant treatments.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 6","pages":" 1543-1553"},"PeriodicalIF":5.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin and vitamin D3 release from calcium phosphate enhances bone regeneration","authors":"Yongdeok Jo, Priya Kushram and Susmita Bose","doi":"10.1039/D4BM01188K","DOIUrl":"10.1039/D4BM01188K","url":null,"abstract":"<p >Improving early <em>in vivo</em> osseointegration and removing residual cancer cells after tumor removal requires the development of novel bone implants with osteogenic and anti-cancer properties. Here, curcumin and vitamin D3 (Cur/VitD3) are loaded into calcium phosphate (CaP) matrices to improve <em>in vivo</em> osteogenesis and inhibit the proliferation of human osteosarcoma cells. Patient-specific, 3D-printed tricalcium phosphate (TCP) loaded with Cur/VitD3 increases the viability of <em>in vitro</em> osteoblast cells after 11 days. When delivered in combination, Cur/VitD3 loaded hydroxyapatite (HA)-coated Ti64 implant promotes new bone formation by 2.7-fold compared to the control after 6 weeks. This delivery system also decreases osteosarcoma cell viability relative to the 3D-printed TCP after day 11, indicating its anti-cancer properties. These findings contribute to the understanding of multifunctional CaP bone grafts to improve early osteogenesis after severe bone trauma and suppress the proliferation of osteosarcoma cells after tumor resection surgery.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 6","pages":" 1568-1577"},"PeriodicalIF":5.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective cell sheet preparation using thermoresponsive polymer brushes with various graft densities and chain lengths†","authors":"Kenichi Nagase, Minami Watanabe, Akihiko Kikuchi and Teruo Okano","doi":"10.1039/D4BM01705F","DOIUrl":"10.1039/D4BM01705F","url":null,"abstract":"<p >Various cell sheets have been used as effective and useful cellular tissues in tissue engineering and regenerative therapy. Poly(<em>N</em>-isopropylacrylamide) (PNIPAAm)-modified surfaces have been investigated for effective cell sheet preparation. In this study, the effective PNIPAAm graft density and chain length of PNIPAAm brushes for various cell types were investigated. The PNIPAAm brush-grafted glass was prepared <em>via</em> silanization and subsequent atom transfer radical polymerization (ATRP). The density of the PNIPAAm brushes was modulated by changing the ATRP initiator and co-adsorber composition, while the PNIPAAm brush length was modulated by changing the monomer concentration in the ATRP. The hydrophilicity of the PNIPAAm brushes increased with increasing PNIPAAm brush length because long PNIPAAm brushes tended to hydrate. Fibronectin adsorption increased with decreasing PNIPAAm brush concentration because the exposed hydrophobic co-adsorber in the dilute PNIPAAm brush enhanced the adsorption of fibronectin. The cell-sheet fabrication ability was investigated using six types of PNIPAAm brushes. An endothelial cell sheet was fabricated using a dense, short PNIPAAm brush. NIH/3T3 sheets can be fabricated using three types of PNIPAAm brushes: dense-long PNIPAAm brushes, moderately dense-short PNIPAAm brushes, and dilute-long PNIPAAm brushes. MDCK cell sheets could not be prepared using the PNIPAAm brushes. A549 cell sheets were prepared using a dense-short PNIPAAm brush and moderately dense-short PNIPAAm brushes. These results indicate that the optimal PNIPAAm brush conditions for cell sheet preparation vary depending on cell type. Thus, modulation of PNIPAAm brush density and length is an effective approach for preparing target cell sheets.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 7","pages":" 1657-1670"},"PeriodicalIF":5.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d4bm01705f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a VEGF-activated scaffold with enhanced angiogenic and neurogenic properties for chronic wound healing applications†","authors":"Juan Carlos Palomeque Chávez, Matthew McGrath, Cian O'Connor, Adrian Dervan, James E. Dixon, Cathal J. Kearney, Shane Browne and Fergal J. O'Brien","doi":"10.1039/D4BM01051E","DOIUrl":"10.1039/D4BM01051E","url":null,"abstract":"<p >Chronic wounds remain in a state of disrupted healing, impeding neurite outgrowth from injured nerves and poor development of new blood vessels by angiogenesis. Current therapeutic approaches primarily focus on the restoration of vascularization and overlook the need of nerve regeneration for complete healing. Vascular endothelial growth factor (VEGF) is a critical growth factor supporting angiogenesis in wound healing, promoting vascularization and has also demonstrated neuro-protective capabilities in both central and peripheral nervous system. While the delivery of pro-regenerative recombinant growth factors has shown promise, gene delivery offers greater stability, reduced off-target side effects, diminished cytotoxicity, and lower production costs. In this context, the overarching goal of this study was to develop a VEGF-activated scaffold with the potential to provide a multifaceted response that enhances both angiogenesis and nerve repair in wound healing through the localized delivery of plasmid encoding VEGF (<em>p</em>VEGF) encapsulated within the GET peptide system. Initially, delivery of <em>p</em>VEGF/GET nanoparticles to dermal fibroblasts led to higher VEGF protein expression without a compromise in cell viability. Transfection of dermal fibroblasts and endothelial cells on the VEGF-activated scaffolds resulted in enhanced VEGF expression, improved endothelial cell migration and organization into vascular-like structures. Finally, the VEGF-activated scaffolds consistently displayed enhanced neurogenic ability through improved neurite outgrowth from neural cells in <em>in vitro</em> and <em>ex vivo</em> models. Taken together, the VEGF-activated scaffold demonstrates multifaceted outcomes through the induction of pro-angiogenic and neurogenic responses from dermal, vascular and neural cells, illustrating the potential of this platform for the healing of chronic wounds.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 1993-2011"},"PeriodicalIF":5.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid lipid nanoparticles derived from human mesenchymal stem cell extracellular vesicles by microfluidic sonication for collagen I mRNA delivery to human tendon progenitor stem cells†","authors":"Rubén Pareja Tello, Erwin Pavel Lamparelli, Maria Camilla Ciardulli, Jouni Hirvonen, Goncalo Barreto, Nicola Mafulli, Giovanna Della Porta and Hélder A. Santos","doi":"10.1039/D4BM01405G","DOIUrl":"10.1039/D4BM01405G","url":null,"abstract":"<p >Tendon degeneration remains an intricate pathological process characterized by the coexistence of multiple dysregulated homeostasis processes, including the increase in collagen III production in comparison with collagen I. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) remain a promising therapeutic tool thanks to their pro-regenerative properties and applicability as drug delivery systems, despite their drug loading limitations. Herein, we developed MSC-EV-derived hybrid lipid nanoparticles (MSC-Hyb NPs) using a microfluidic-sonication technique as an alternative platform for the delivery of collagen type I (COL 1A1) mRNA into pathological TSPCs. The MSC-Hyb NPs produced had LNP-like physicochemical characteristics and were 178.6 nm in size with a PDI value of 0.245. Moreover, MSC-Hyb NPs encapsulated mRNA and included EV-derived surface proteins such as CD63, CD81 and CD144. MSC-Hyb NPs remained highly biocompatible with TSPCs and proved to be functional mRNA delivery agents with certain limitations in comparison with lipid nanoparticles (LNPs). <em>In vitro</em> efficacy studies on TSPCs showed a 2-fold increase in procollagen type I carboxy-terminal peptide production comparable with the effect caused by LNPs. Therefore, our work provides an alternative production method for MSC-EV-derived hybrid NPs and supports their potential use as drug delivery systems for tendon regeneration.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 8","pages":" 2066-2081"},"PeriodicalIF":5.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d4bm01405g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced combination therapy through tumor microenvironment-activated cellular uptake and ROS-sensitive drug release using a dual-sensitive nanogel†","authors":"Jianming Yuan, Qinfeng Chen, Mingxiang Zuo, Xiaoxia Li, ChiYi Ou, Qinghua Chen, Dongsheng Yu, Haowen Li, Chenhui Hao, Jing Yang, Shuang Liu and Du Cheng","doi":"10.1039/D4BM01377H","DOIUrl":"10.1039/D4BM01377H","url":null,"abstract":"<p >Although the co-delivery of chemotherapeutic and photodynamic agents has been studied for years, developing a simple and efficient nanoplatform for high co-delivery efficiency remains a challenge for clinical applications. In this study, we prepared a reactive oxygen species (ROS) and pH dual-sensitive nanogel for the co-encapsulation of doxorubicin (DOX) and indocyanine green (ICG)-conjugated bovine serum albumin (BSA) <em>via</em> a simple inverse miniemulsion polymerization process. This was followed by modification with pegylated cell-penetrating peptides (CPPs) containing citraconic anhydride (CDM) linkers, which are sensitive to weakly acidic microenvironments (pH 6.5). Pegylation endowed the nanogel with extended blood circulation, while the de-shielding of polyethylene glycol (PEG) exposed the CPPs, significantly enhancing cellular uptake. Upon near-infrared (NIR) irradiation, ROS generated by ICG not only killed tumor cells but also triggered the release of DOX through nanogel disintegration. Serial experiments verified the nanogel's high co-delivery efficiency, tumor tissue matrix microenvironment-triggered cellular uptake, controlled drug release, and synergistic antitumor effects. Therefore, this dual-sensitive nanogel, prepared <em>via</em> inverse miniemulsion polymerization, offers a facile approach to improving co-delivery efficiency for combination therapy.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 6","pages":" 1554-1567"},"PeriodicalIF":5.8,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d4bm01377h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Unraveling the mystery: effect of trapped air on platelet adhesion on hydrophobic nanostructured titanium dioxide","authors":"Zhenyu Shen, Ke Wu, Zhiwei Chen, Yun Yang and Qiaoling Huang","doi":"10.1039/D5BM90012C","DOIUrl":"10.1039/D5BM90012C","url":null,"abstract":"<p >Correction for ‘Unraveling the mystery: effect of trapped air on platelet adhesion on hydrophobic nanostructured titanium dioxide’ by Zhenyu Shen <em>et al.</em>, <em>Biomater. Sci.</em>, 2025, <strong>13</strong>, 627–638, https://doi.org/10.1039/D4BM01143K.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 5","pages":" 1355-1355"},"PeriodicalIF":5.8,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm90012c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of ionizable groups in star polymer nanoparticles on NLRP3 inflammasome activation†","authors":"Mehak Malhotra, Sarmishta Thodur and Ashish Kulkarni","doi":"10.1039/D4BM01349B","DOIUrl":"10.1039/D4BM01349B","url":null,"abstract":"<p >The advent of cancer nanovaccines (N.V.s) has transformed immunotherapy by using nanoparticles as biologic delivery vehicles or vaccine adjuvants. However, challenges remain due to nanoparticle-immune cell interactions. Investigating nanoparticle (N.P.) physicochemical effects on the innate immune system is crucial for safe biomaterials design. The NLRP3 inflammasome, a key innate immunity component, is implicated in many inflammatory disorders. Various nanoparticle-associated molecular patterns (NAMPs) trigger NLRP3 activation, but the combined effect of these NAMPs in a single N.P. platform is not well understood. Star polymer nanocarriers were chosen to study the impact of combined hydrophobic and ionizable groups on NLRP3 activation. Star polymers offer stable self-assembly, high drug/gene encapsulation, and enhanced cellular internalization. We designed 4-arm star random copolymers with constant hydrophobic moiety and varied ionizable groups to evaluate their NLRP3 activation in macrophages. The study revealed differences in cytokine release and cell death linked to ionizable groups, providing insights for selecting safe, immunomodulatory biomaterials.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 7","pages":" 1709-1720"},"PeriodicalIF":5.8,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}