Biomaterials Science最新文献

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Saponin-mediated cell membrane interference nanomedicine potentiates tumor chemo-immunotherapy via a perforin-granzyme-like mechanism† 皂苷介导的细胞膜干扰纳米药物通过穿孔颗粒酶样机制增强肿瘤化学免疫治疗。
IF 5.8 3区 医学
Biomaterials Science Pub Date : 2025-06-03 DOI: 10.1039/D5BM00564G
Jingsong Lu, Baoli Ma, Ying Li, Sumei Chen, Muyan Zhang, Zhenhu Guo, Xiaohan Gao, Ufurahi-Pambe Neema, Abdul Fahad, Wensheng Xie, Xiaodan Sun, Xiumei Wang, Guifeng Zhang, Jing Yu, Shenglei Che, Yen Wei and Lingyun Zhao
{"title":"Saponin-mediated cell membrane interference nanomedicine potentiates tumor chemo-immunotherapy via a perforin-granzyme-like mechanism†","authors":"Jingsong Lu, Baoli Ma, Ying Li, Sumei Chen, Muyan Zhang, Zhenhu Guo, Xiaohan Gao, Ufurahi-Pambe Neema, Abdul Fahad, Wensheng Xie, Xiaodan Sun, Xiumei Wang, Guifeng Zhang, Jing Yu, Shenglei Che, Yen Wei and Lingyun Zhao","doi":"10.1039/D5BM00564G","DOIUrl":"10.1039/D5BM00564G","url":null,"abstract":"<p >Pore formation can facilitate the release of various intracellular substances upon cell death, which is of critical benefit in tumor immunotherapy by immunogenic cell death (ICD). Given that effective endogenous antigen release is of primary importance for ICD-eliciting immunogenicity, in addition to enhancing the cytotoxicity of ICD, we designed an immunogenic induction strategy <em>via</em> pore formation based on saponin, a composite medium of membrane-disrupting agents. In this study, saponin/polyphenol (ZS-TA) at appropriate concentrations directly caused membrane perforation by removing cholesterol from the membrane, thereby leading to the release of intracellular substances. Simultaneously, <em>in situ</em> nano-antigens (nano-Ags) were formed through the mechanism of protein-polyphenol interaction, and the nano-Ag served as a reservoir of antigens to trigger long-term immune effects. Meanwhile, this membrane perforation enhanced the uptake of chemotherapeutic drugs, serving as a general approach for drug delivery. Therefore, this work provides insights into the design of enhanced drug delivery systems and <em>in situ</em> vaccines to sensitize tumor chemo-immunotherapy.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 14","pages":" 3942-3957"},"PeriodicalIF":5.8,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Conductive and piezoelectric biomaterials: a comprehensive review of load-bearing soft tissue repair 导电和压电生物材料:承载软组织修复的综合综述。
IF 5.8 3区 医学
Biomaterials Science Pub Date : 2025-05-30 DOI: 10.1039/D5BM00368G
Wenjie Wu, Huihui Yang, Tingting Li, Yang Xie, Guoyou Huang and Weiwei Zhang
{"title":"Conductive and piezoelectric biomaterials: a comprehensive review of load-bearing soft tissue repair","authors":"Wenjie Wu, Huihui Yang, Tingting Li, Yang Xie, Guoyou Huang and Weiwei Zhang","doi":"10.1039/D5BM00368G","DOIUrl":"10.1039/D5BM00368G","url":null,"abstract":"<p >Load-bearing soft tissues, such as tendons, cartilage, and ligaments, withstand substantial mechanical stress and are susceptible to injury, particularly in athletes. The increasing prevalence of these injuries poses a significant challenge, exacerbated by the limitations of traditional treatments, which often lead to lengthy recovery periods and a high risk of recurrence. In recent years, researchers have harnessed the electrical properties of conductive and piezoelectric biomaterials to address challenges in load-bearing soft tissue engineering. These materials facilitate electrical stimulation and/or enable the monitoring of biomechanical properties during motion, with the aim of advancing load-bearing soft tissue regeneration and repair. This review explores the roles and mechanisms of electrical cues in load-bearing soft tissues, highlighting the development and application of two primary types of biomaterials—conductive and piezoelectric materials—in electro-biomechanical sensing and stimulation therapies for load-bearing soft tissue engineering.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 14","pages":" 3755-3771"},"PeriodicalIF":5.8,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00368g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protein corona as the key factor governing the in vivo fate of magnetic nanoparticles† 蛋白质电晕是控制磁性纳米颗粒体内命运的关键因素。
IF 5.8 3区 医学
Biomaterials Science Pub Date : 2025-05-29 DOI: 10.1039/D5BM00387C
Ashish Avasthi, Yilian Fernandez-Afonso, Lucía Gutiérrez, Jesús M. De la Fuente, Manuel Pernía Leal, Carlos Caro and Maria Luisa Garcia-Martin
{"title":"Protein corona as the key factor governing the in vivo fate of magnetic nanoparticles†","authors":"Ashish Avasthi, Yilian Fernandez-Afonso, Lucía Gutiérrez, Jesús M. De la Fuente, Manuel Pernía Leal, Carlos Caro and Maria Luisa Garcia-Martin","doi":"10.1039/D5BM00387C","DOIUrl":"10.1039/D5BM00387C","url":null,"abstract":"<p >Advancements in the field of nanotechnology have opened a myriad of avenues for diverse applications. One such avenue is the role of nanoparticles (NPs) in the healthcare sector, whether it is drug targeting, drug delivery, or imaging, offering unprecedented prospects for improving targeted interventions along with minimal toxicity. Meanwhile, the intricate interplay between the characteristics of NPs and the ensuing biological cross-talk has engendered profound interest among scientists. Amidst the determinants shaping the behavior of NPs within the biological milieu, the biodistribution and pharmacokinetics of the NPs stand as pivotal factors intricately intertwined with their core size, hydrodynamic diameter (HD), coating ligands, as well as the proteins they interact with, forming the protein corona. This article compiles and analyzes the data to decipher the factors determining the fate of NPs <em>in vivo</em>. For this purpose, two IONPs with differing core sizes (≈4 nm and ≈8 nm) but coated with the same gallol-PEG ligand (GA-PEG3000-OH) and possessing very similar HDs (≈35 nm) as well as relaxivity (≈100 mM<small><sup>−1</sup></small> s<small><sup>−1</sup></small>) were selected. Following physicochemical characterization, both protein coronas were thoroughly analyzed, revealing differences in both the composition and the relative abundance. Later, after determining the negligible cytotoxicity of both NPs, they were intravenously injected into Balb/c mice to evaluate their <em>in vivo</em> biodistribution and pharmacokinetics using MRI. Additionally, biodistribution was further investigated <em>ex vivo</em> by quantitative magnetic analysis of blood and tissues, alongside histological evaluation. Our results evidenced that the protein corona, rather than core size or hydrodynamic diameter, is the determining factor governing the <em>in vivo</em> fate of magnetic NPs.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 14","pages":" 3840-3852"},"PeriodicalIF":5.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Borax - and tannic acid-based post-3D-printing treatment to tune the mechanical properties of scaffolds† 以硼砂和单宁酸为基础的3d打印后处理,以调整支架的机械性能。
IF 5.8 3区 医学
Biomaterials Science Pub Date : 2025-05-28 DOI: 10.1039/D5BM00151J
Julia Simińska-Stanny, Parinaz Hobbi, Pejman Ghaffari-Bohlouli, Man Li, Adam Junka, Hafez Jafari, Christine Delporte, Lei Nie and Armin Shavandi
{"title":"Borax - and tannic acid-based post-3D-printing treatment to tune the mechanical properties of scaffolds†","authors":"Julia Simińska-Stanny, Parinaz Hobbi, Pejman Ghaffari-Bohlouli, Man Li, Adam Junka, Hafez Jafari, Christine Delporte, Lei Nie and Armin Shavandi","doi":"10.1039/D5BM00151J","DOIUrl":"10.1039/D5BM00151J","url":null,"abstract":"<p >Digital light processing (DLP) methods are constrained by the narrow range of cell-compatible resins, limiting their use in biomedical applications that require varied mechanical and biofunctional properties. Current bioresins based on natural polymers such as methacrylated gelatine or alginate usually lack sufficient stretchability and toughness. In this study, we propose a post-processing strategy to tune the mechanical and functional properties of a DLP printable polyethylene glycol diacrylate (PEGDA)/polyvinyl alcohol (PVA) resin <em>via</em> simple treatment with 5% (w/v) tannic acid (TA) or borax (B). The TA treatment reduced the resin's toughness by ∼17% and compressive modulus by ∼16%, while B treatment increased the toughness by ∼53% and the compressive modulus by ∼44% compared with non-treated hydrogels. TA-treated hydrogels continuously released over 59% of the loaded TA, demonstrating antibacterial and radical scavenging activities. Moreover, TA-treated hydrogels, DLP-printed in a tubular shape, demonstrated the highest durability, remaining intact for ∼32 cycles before failure, which was ∼17 cycles more than that for the non-treated hydrogels. Our <em>in vivo</em> larval model further confirmed the hydrogels’ biocompatibility. This study offers a practical approach for post-fabrication tuning of the mechanical and bioactive properties of DLP-printed PEGDA-PVA hydrogels, expanding the utility of existing resins for potential biomedical applications, such as soft tissue engineering.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 13","pages":" 3689-3706"},"PeriodicalIF":5.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A core–shell structured biphasic microneedle system as an elite squad for combating melanoma with “three-in-one” therapeutic power† 核-壳结构双相微针系统是对抗黑色素瘤的精锐团队,具有“三合一”的治疗能力。
IF 5.8 3区 医学
Biomaterials Science Pub Date : 2025-05-27 DOI: 10.1039/D5BM00371G
Qiling Jin, Ying Wang, Wenwen Lei, Shuyao Zhou, Tingting Zhang, Keqiang Lu, Lingzhi Zhao, Wenying Zhong and Keming Xu
{"title":"A core–shell structured biphasic microneedle system as an elite squad for combating melanoma with “three-in-one” therapeutic power†","authors":"Qiling Jin, Ying Wang, Wenwen Lei, Shuyao Zhou, Tingting Zhang, Keqiang Lu, Lingzhi Zhao, Wenying Zhong and Keming Xu","doi":"10.1039/D5BM00371G","DOIUrl":"10.1039/D5BM00371G","url":null,"abstract":"<p >Achieving optimal therapeutic outcomes with microneedle (MN) technology requires a high drug payload, tunable mechanical strength, and robust drug stability—key attributes in demand for transdermal drug delivery. This work introduces a core–shell structured biphasic MN system designed to combat melanoma with “three-in-one” therapeutic power. The MN base, made of water-insoluble poly(methyl methacrylate), forms a biphasic interface with the needle body. Acting as a “shield”, the base effectively prevents drug migration and enhances the drug-loading capacity of the needle body. The needle body features a core–shell design, with a shell composed of photo-cross-linked hydrogel. This shell serves as a “spear” to optimize mechanical properties of MNs, efficiently piercing the skin barrier. Meanwhile, the core section of MN, constructed from hyaluronic acid, acts as a “bow and arrow” to preserve the bioactivity of chlorin e6 nanoparticles for launching an effective “attack” on melanoma cells through photodynamic therapy. The MN system demonstrates exceptional mechanical performance and enhanced anticancer efficacy against melanoma cells both <em>in vitro</em> and <em>in vivo</em>. In summary, this study introduces a new “elite squad” strategy that integrates three critical functionalities into a single MN platform, offering significant potential for treating melanoma and other malignant skin conditions.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 13","pages":" 3662-3677"},"PeriodicalIF":5.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A study of saponin-encapsulated ultrasound microbubbles Rb3NPs@MBs for atherosclerosis targeted treatment. 皂素包封超声微泡Rb3NPs@MBs用于动脉粥样硬化靶向治疗的研究。
IF 5.8 3区 医学
Biomaterials Science Pub Date : 2025-05-27 DOI: 10.1039/d5bm00078e
Chunting Zhong, Jianhua Bai, Xiaoting Yang, Yiran Ji, Jiabao Huang, Xiao Tan, Xiaoyu Chen, LiJun Xing, Bingxuan Xu, Dianhuan Tan, Yun Chen, Tingting Zheng
{"title":"A study of saponin-encapsulated ultrasound microbubbles Rb<sub>3</sub>NPs@MBs for atherosclerosis targeted treatment.","authors":"Chunting Zhong, Jianhua Bai, Xiaoting Yang, Yiran Ji, Jiabao Huang, Xiao Tan, Xiaoyu Chen, LiJun Xing, Bingxuan Xu, Dianhuan Tan, Yun Chen, Tingting Zheng","doi":"10.1039/d5bm00078e","DOIUrl":"https://doi.org/10.1039/d5bm00078e","url":null,"abstract":"<p><p>Atherosclerosis remains a leading disease posing significant threats to human health and life. Oxidative stress plays a critical role in the initiation of early atherosclerosis. Ginsenoside Rb<sub>3</sub> has been shown to exert potential therapeutic effects against atherosclerosis due to its antioxidant properties. However, its clinical utility remains constrained to the nanometer scale, offering insufficient targeting capability for atherosclerosis treatment. To address this limitation, we designed a novel Rb<sub>3</sub>-loaded microbubble system Rb<sub>3</sub>NPs@MBs. This microbubble system effectively encapsulates Rb<sub>3</sub> nanoparticles and, <i>via</i> ultrasound-targeted microbubble destruction (UTMD), facilitates their targeted accumulation in the aortic arch of atherosclerotic mice. Subsequently, Rb<sub>3</sub>NPs@MBs reduce oxidative stress, attenuate endothelial cell apoptosis and foam cell formation, and ultimately diminish plaque development at the lesion site. This strategy holds promise as a therapeutic approach for atherosclerosis. These findings suggest that Rb<sub>3</sub>NPs@MBs represent a promising therapeutic strategy for atherosclerosis.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fabrication of uniform biodegradable microcages with predesigned shape printed from microarrays for sustained release of small hydrophilic molecules† 由微阵列打印的具有预先设计形状的均匀可生物降解微笼的制造,用于持续释放小亲水分子。
IF 5.8 3区 医学
Biomaterials Science Pub Date : 2025-05-27 DOI: 10.1039/D5BM00154D
Jiaxin Zhang, Rui Sun, Valeriya Kudryavtseva, David J. Gould and Gleb B. Sukhorukov
{"title":"Fabrication of uniform biodegradable microcages with predesigned shape printed from microarrays for sustained release of small hydrophilic molecules†","authors":"Jiaxin Zhang, Rui Sun, Valeriya Kudryavtseva, David J. Gould and Gleb B. Sukhorukov","doi":"10.1039/D5BM00154D","DOIUrl":"10.1039/D5BM00154D","url":null,"abstract":"<p >Drug delivery vehicles have aroused increasing attention over the years due to their ability to protect and control the release of encapsulated cargo. However, several challenges significantly limit their wide applications including poor size distribution, uncontrollable size and shape, and leakage of loaded small hydrophilic cargos. This work introduces a novel and scalable microarray-based printing technique for preparing uniform biodegradable “microcages” with predesigned shapes for encapsulating and controlling the release of small hydrophilic molecules. The drugs encapsulated in the microcage are centrally located within solid microparticles without being exposed to the surface or dispersed throughout the polymer matrix. Here, 5(6)-carboxyfluorescein (CF) as a small and hydrophilic model drug are successfully loaded into polylactide acid (PLA) microcages with the dry loading method. Additionally, blending polycaprolactone (PCL) with PLA increases the permeability of the microcage polymer shells for controlled release. A higher PCL content results in a faster release rate of the encapsulated drug. Approximately 28 pg of CF particles can be encapsulated within individual microcages. This microcage printing technique provides a novel, scalable method for producing uniform biodegradable microcages, extending microprinting beyond microfilms and microparticles. A unique dry loading approach, independent of drug solubility, further broadens its utility for diverse biomedical applications.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 13","pages":" 3678-3688"},"PeriodicalIF":5.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/bm/d5bm00154d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PISA printing perfusable microcapillaries. PISA打印可灌注毛细血管。
IF 5.8 3区 医学
Biomaterials Science Pub Date : 2025-05-27 DOI: 10.1039/d5bm00547g
Aaron Priester, Jimmy Yeng, Yuwei Zhang, David Christofferson, Risheng Wang, Anthony J Convertine
{"title":"PISA printing perfusable microcapillaries.","authors":"Aaron Priester, Jimmy Yeng, Yuwei Zhang, David Christofferson, Risheng Wang, Anthony J Convertine","doi":"10.1039/d5bm00547g","DOIUrl":"https://doi.org/10.1039/d5bm00547g","url":null,"abstract":"<p><p>Polymerization-induced self-assembly (PISA) printing combines reversible addition-fragmentation chain transfer (RAFT) polymerization with digital light projection (DLP) photolithography to create high-resolution three-dimensional structures without permanent covalent crosslinks. Here, we intoduce a simplified, one-pot, purification-free synthesis for multi-chain transfer agent (multi-CTA) scaffolds that spontaneously form robust physical networks durnig printing, stabilized by interparticle bridges and knots. By tuning solvent-resin chemistry and polymer composition, we achieved precise control over nanoscale morphologies and selective distribution behaviors. This approach was demonstrate through successful fabrication of perfusable microvascular networks and open-channel polydimethylsiloxane (PDMS) microfluidic devices, where sacrificial scaffolds dissolved cleanly to yield stable microchannels. Collectively, these findings enhance the accessibliity, flexibility, and functionality of PISA printing, offering an efficient and adaptable platform for microfabrication, rapid prototyping, and advance d tissue engineering applications.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Designing antibacterial polymeric systems: (co)poly(2-oxazoline) conjugates with acyclic and macrocyclic polyamino polycarboxylic chelators† 抗菌高分子体系的设计:(co)聚(2-恶唑啉)与无环和大环多氨基多羧基螯合剂的偶联。
IF 5.8 3区 医学
Biomaterials Science Pub Date : 2025-05-23 DOI: 10.1039/D5BM00270B
Marcelina Bochenek, Barbara Mendrek, Agnieszka Kowalczuk, Wojciech Wałach, Łukasz Jałowiecki, Jacek Borgulat, Grażyna Płaza, Jerzy Kubacki, Marcin Sikora, Agnieszka Fus-Kujawa, Łukasz Sieroń, Katarzyna Gawron and Natalia Oleszko-Torbus
{"title":"Designing antibacterial polymeric systems: (co)poly(2-oxazoline) conjugates with acyclic and macrocyclic polyamino polycarboxylic chelators†","authors":"Marcelina Bochenek, Barbara Mendrek, Agnieszka Kowalczuk, Wojciech Wałach, Łukasz Jałowiecki, Jacek Borgulat, Grażyna Płaza, Jerzy Kubacki, Marcin Sikora, Agnieszka Fus-Kujawa, Łukasz Sieroń, Katarzyna Gawron and Natalia Oleszko-Torbus","doi":"10.1039/D5BM00270B","DOIUrl":"10.1039/D5BM00270B","url":null,"abstract":"<p >In this work, we propose a new synthetic pathway to obtain conjugates of poly(2-oxazoline)s (POx) and chelating agents (CA) with antibacterial activity, applying a triazine-based coupling compound. The copolymers based on 2-ethyl-2-oxazoline (EtOx) and 2-(3-butenyl)-2-oxazoline (ButEnOx) were coupled with acyclic and macrocyclic chelating compounds, <em>i.e.</em>, diethylenetriaminepentaacetic acid (DTPA) and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA). The obtained systems were named as POx-DTPA and POx-DOTA, respectively. This modification provided an opportunity to impart to the designed macromolecules the ability for complexation of ions, which stabilize the membrane of bacterial cell. We present, for the first time, studies on the uptake of various cations, including those stabilizing the membrane of Gram-negative bacteria at different environmental pH values for POx-DTPA and POx-DOTA macromolecules, where the increase in the efficiency of conjugate ion trapping is possible owing to the ionization of the functional groups of CA coupled to POx. The antibacterial properties of the designed systems were also confirmed by assessing their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) with the use of Gram-negative strains of <em>E. coli</em> and <em>P. aeruginosa</em>. Using cytometric analysis, we further investigated the ability of the obtained systems to disrupt the bacterial cell membrane, demonstrating the permeability of the outer bacterial membrane (OM) after POx-DOTA treatment, and the disruption of the OM and the inner membrane (IM) after POx-DTPA treatment. The designed macromolecular systems also remained non-toxic to human skin fibroblasts in a wide range of concentrations, making them promising candidates for further studies on antibacterial biomaterials.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 14","pages":" 3876-3886"},"PeriodicalIF":5.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A collagen hydrogel-based intestinal model enabling physiological epithelial–immune cell interactions in host–microbe studies† 一种基于胶原水凝胶的肠道模型,可在宿主-微生物研究中实现生理上皮-免疫细胞相互作用。
IF 5.8 3区 医学
Biomaterials Science Pub Date : 2025-05-21 DOI: 10.1039/D5BM00612K
Namju Kim, Jiwon Kim, Jonghoon Choi and Raehyun Kim
{"title":"A collagen hydrogel-based intestinal model enabling physiological epithelial–immune cell interactions in host–microbe studies†","authors":"Namju Kim, Jiwon Kim, Jonghoon Choi and Raehyun Kim","doi":"10.1039/D5BM00612K","DOIUrl":"10.1039/D5BM00612K","url":null,"abstract":"<p >The human intestine orchestrates complex immune responses to external stimuli, including trillions of gut microbes. Understanding host–microbiota interactions remains challenging due to the complex cellular composition of intestinal tissues. In this study, we present InTRIC (Intestinal model with Tissue-Resident Immune Components), a three-dimensional intestinal model incorporating tissue-resident immune cells within a biomimetic extracellular matrix. We developed a hydrogel system by combining UV-crosslinked methacrylated collagen with a native collagen blend that prevents matrix shrinkage while maintaining physiologically relevant mechanical properties, a fibrillar microstructure and minimal toxicity for cell embedding. Prior to establishing InTRIC, we validated THP-1 viability in the collagen hydrogel (&gt;14 days), verified their differentiation into distinct macrophage phenotypes, and confirmed appropriate gene expression in Caco-2 cells on the collagen substrates. The integrated model comprises THP-1 macrophages embedded within the collagen matrix and Caco-2 cells forming a functional epithelium with intact junctions and physiologically relevant TEER values. The InTRIC platform enables both the assessment of cytokine profiles and the visualization of transepithelial macrophage infiltration in response to commensal (<em>Lacticaseibacillus rhamnosus</em>) and pathogenic (<em>Pseudomonas aeruginosa</em>) bacteria. Notably, <em>P. aeruginosa</em> exposure induced a four-fold increase in macrophage infiltration and elevated IL-8 secretion. Compared to conventional cocultures without collagen, InTRIC exhibited attenuated inflammatory cytokine secretion, suggesting microenvironmental modulation of epithelial–immune communication. Additionally, M2-polarized macrophages showed reduced basal IL-8 secretion but increased responsiveness to bacterial stimulation. This proof-of-concept model demonstrates the importance of incorporating both immune cells and appropriate extracellular matrices in recapitulating intestinal conditions and offers a physiologically relevant test platform for applications in drug discovery, toxicology, and microbiome research.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 14","pages":" 3887-3902"},"PeriodicalIF":5.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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