Food & Function最新文献

{"title":"Efficacy of vitamin E from dietary, circulation system, and supplementation on chronic obstructive pulmonary disease (COPD): a systematic review and meta-analysis.","authors":"Peixuan Tian, Hui Xia, Bihuan Hu, Yu Yang, Da Pan, Wang Liao, Shaokang Wang, Guiju Sun, Jing Sui","doi":"10.1039/d5fo03814f","DOIUrl":"https://doi.org/10.1039/d5fo03814f","url":null,"abstract":"<p><p><i>Background</i>: In recent years, the efficacy of antioxidants in the management of various diseases has garnered significant attention. Vitamin E, a natural supplement with potent antioxidant properties, has been utilized in the prevention of chronic obstructive pulmonary disease (COPD) and the treatment of patients with COPD. This systematic review and meta-analysis aimed to clarify the association between vitamin E consumption and COPD. <i>Methods</i>: We conducted a systematic search of eligible studies from PubMed, Web of Science, Scopus, and the Cochrane Library databases, performed a meta-analysis using random-effects models and assessed the association of vitamin E dietary intake, serum levels, and supplementation with COPD. <i>Results</i>: The study included a total of 10 studies involving 65 425 participants. The meta-analysis results indicated that the odds ratio (OR) between vitamin E intake and the onset risk of COPD was 0.60 (95% CI: 0.46-0.80, <i>P</i> < 0.0001), which indicated a significant protective effect. However, no significant correlation was observed between vitamin E consumption and the risk of death from COPD (OR = 0.79, 95% CI = 0.55-1.12, <i>P</i> = 0.07). In addition, subgroup analysis revealed that different subtypes of vitamin E, such as vitamin E (OR = 0.78, 95% CI = 0.68-0.91, <i>P</i> = 0.01) and α-tocopherol (OR = 0.43, 95% CI = 0.23-0.84, <i>P</i> = 0.03), as well as various sources of vitamin E, including dietary intake (OR = 0.68, 95% CI = 0.54-0.86, <i>P</i> = 0.007), serum levels (OR = 0.49, 95% CI = 0.25-0.98, <i>P</i> = 0.02), and supplementation (OR = 0.90, 95% CI = 0.82-0.99, <i>P</i> = 0.03) were all significantly inversely associated with COPD. <i>Conclusion</i>: Vitamin E consumption, including the intake of different subtypes and from various supplementation sources, demonstrates a protective effect against the risk of COPD and may contribute to improved clinical outcomes in patients with COPD. However, further research is required to fully elucidate the potential role and mechanism of vitamin E in COPD.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of the GlucoTRIG index to rank the postprandial metabolic responses to composite meals in healthy adults - a randomized, controlled, crossover trial.","authors":"Wen Xin Janice Lim, Paul J Moughan, Pamela von Hurst, Conlon A Cathryn, Harjinder Singh, Manohar Garg","doi":"10.1039/d5fo02882e","DOIUrl":"https://doi.org/10.1039/d5fo02882e","url":null,"abstract":"<p><p>GlucoTRIG is a recently developed index that incorporates postprandial insulin and triglyceride responses to quantify the potential metabolic effects of composite meals that may be associated with long-term health. The study was a randomized, controlled, crossover trial involving healthy adults (<i>n</i> = 12, M: 2, F: 10), aged 25.5 ± 1.6 years, with BMI 21.9 ± 0.7 kg m^-2, HbA1c 31 ± 1 mmol mol^-1, and fasting triglyceride (FTG) 1.2 ± 0.1 mmol L^-1. Three isocaloric meals (2000 kJ) were examined: reference (Ref) meal (17% protein, 26% fat, 55% carbohydrate, 0.6% fiber), high-carbohydrate, low-fat (HCLF) meal (17% protein, 13% fat, 68% carbohydrate, 0.9% fiber), and high-fat, low-carbohydrate (HFLC) meal (19% protein, 51% fat, 29% carbohydrate, 1.1% fiber). A baseline blood sample was taken followed by a further blood sample collection after the meal at 180 min. The GlucoTRIG value was calculated as [plasma triglyceride_180min (mmol L^-1) × plasma insulin_180min (pmol L^-1)] - [plasma triglyceride_0min (mmol L^-1) × plasma insulin_0min (pmol L^-1)]. Postprandial triglyceride (PPTG) changes from baseline were significantly different among the three meals: Ref (0.1 ± 0.04 mmol L^-1), HCLF (0.3 ± 0.1 mmol L^-1), and HFLC (0.6 ± 0.1 mmol L^-1), <i>p</i> < 0.001. Significant postprandial insulin (PPI) changes from baseline were also observed: Ref (13.7 ± 6.9 pmol L^-1), HCLF (28.9 ± 7.3 pmol L^-1), and HFLC (5.7 ± 4.0 pmol L^-1), <i>p</i> = 0.032. The GlucoTRIG values were: Ref (14 ± 6.5), HCLF (38 ± 7.7), and HFLC (36 ± 6.1), <i>p</i> < 0.001, with the Ref meal value being significantly lower than for the HCLF (<i>p</i> = 0.011) and the HFLC (<i>p</i> = 0.024) meals. The GlucoTRIG index was able to differentiate between the plasma metabolic profiles of meals with different macronutrient contents. Heightened postprandial blood levels of insulin and triglycerides may be related to long-term health outcomes.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From microbiota to metabolomics: how <i>Bifidobacterium infantis</i> YLGB-1496 shields neonates from necrotizing enterocolitis.","authors":"Jingyu Li, Hanglian Lan, Yue Liu, Xueer Wang, Shixiang Wu, Wen Zhao, Wei-Lian Hung, Jian He, Bing Fang, Ming Zhang","doi":"10.1039/d5fo03645c","DOIUrl":"https://doi.org/10.1039/d5fo03645c","url":null,"abstract":"<p><p><i>Bifidobacterium</i> species are recognized as essential primary colonizers of the infant gastrointestinal tract and constitute a critical component of infant formula. Nevertheless, the precise mechanisms by which <i>Bifidobacterium</i> confers its protective effects against gut inflammation remain insufficiently elucidated. In an experimental model of Necrotizing Enterocolitis (NEC), we observed that human milk-derived <i>Bifidobacterium longum subsp. infantis</i> YLGB-1496 (GB1496) demonstrated a significant protective effect against mortality, intestinal damage, and TLR-4-mediated inflammatory responses in neonatal mice. Subsequent analyses of the intestinal microbiota indicated that oral administration of GB1496 during the preventive phase markedly increased the abundance of <i>Lactobacillus</i> and <i>Bifidobacterium</i>. Furthermore, serum untargeted metabolomics revealed significant alterations in the levels of tryptophan metabolites. Specific tryptophan metabolites, such as indole-3-acetic acid, L-formylkynurenine, and 5-hydroxyindole-3-acetic acid, were significantly enriched following GB1496 intervention, potentially activating the AHR-associated anti-inflammatory pathway as ligands. Correlation analysis indicates a potential association between GB1496-induced Bifidobacterium enrichment and enhanced intestinal tryptophan metabolism. The collective impact of GB1496 intervention has been demonstrated to engender an augmentation in Bifidobacterium abundance, a concomitant downregulation of inflammatory factors associated with the TLR-4 pathway, and an induction of alterations in serum tryptophan metabolites. It is evident that these combined effects contribute to its protective action against NEC.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The differential alleviation of DSS-induced colitis in mice by black tea extracts of different origins <i>via</i> enhancing the gut barrier and gut microbiota modulation.","authors":"Yina Huang, Yao He, Yunjie Cai, Yubin Chen, Xunmei Shi, Jingyu He, Kesheng Wu, Hua Wei","doi":"10.1039/d5fo02993g","DOIUrl":"https://doi.org/10.1039/d5fo02993g","url":null,"abstract":"<p><p>Black tea is commonly used for tea beverage production and has been shown to be an effective natural ingredient to prevent experimentally induced colitis. However, there is limited evidence to show whether black teas of different origins demonstrate similar anti-inflammatory capacity in mice with colitis. In this study, mice were administered daily black tea extracts of lapsang, keemun or dianhong for 1 week prior to receiving 4% dextran sulfate sodium for inducing colitis. Both the lapsang and keemun extracts exhibited better anti-inflammatory effects than dianhong extract, as evidenced by the former two tea extracts enhancing intestinal barrier functions (up-regulation of MUC2 and ZO-1 and increased population of goblet cells) and decreasing colonic and serum pro-inflammatory cytokines. We deduced that gallocatechin (GC) might be a key contributor to the anti-colitis effects of black tea, potentially through synergistic interactions with other components at an optimal ratio to enhance the anti-inflammatory efficacy. Fecal microbiome analysis showed that the gut microbiome was differentially modulated by the lapsang and keemun extracts. Their anti-colitic effects were dependent on the gut microbiome, as shown by the loss of such protection in DSS mice treated with broad-spectrum antibiotics (ABX) for significant microbiome alterations. Mechanistically, colonic transcriptomic analysis showed the differential impacts of lapsang and keemun extracts on colitis <i>via</i> modulating the gene expressions of the glutamatergic synapse and IL-17 pathway, respectively. Further qPCR and immunohistochemistry assays verified the aforementioned pathway modulation. Together, our study provides a roadmap for understanding the effects of different black tea types on colitis and for providing potential directions for the nutritional modulation of colitis.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mung bean (<i>Vigna radiata</i> L.) ethanol extract alleviates alcoholic liver injury with modulation of the gut microbiota and spermidine elevation.","authors":"Yixin Liu, Yi Ruan, Anhui Zhao, Zhiying Bian, Jing Bai, Shuqi Niu, Songtao Wang, Zhangjun Huang, Xiaonian Cao, Caihong Shen, Sijing Liu, Jinlin Guo","doi":"10.1039/d5fo02499d","DOIUrl":"https://doi.org/10.1039/d5fo02499d","url":null,"abstract":"<p><p>The pathophysiology of alcohol-associated liver disease (ALD) is multifaceted. Utilizing the gut-liver axis framework and integrated multi-omics approaches, this study systematically evaluated the therapeutic potential of mung bean (<i>Vigna radiata</i> L.) ethanol extract (MBE) in ALD and its regulatory effects on the gut microbiota and serum metabolites. Chemical analysis identified vitexin, isovitexin, catechin, trigonelline, and caffeic acid as MBE's primary bioactive compounds. In a modified NIAAA model, MBE alleviated liver injury, lipid dysregulation, inflammation, oxidative stress, and gut barrier damage <i>via</i> PPARα-mediated lipid metabolism and Nrf2-driven antioxidant activation. 16S rRNA sequencing and an <i>in vitro</i> fermentation experiment revealed that MBE specifically enriched <i>Lactobacillus johnsonii in vivo</i> and promoted its growth <i>in vitro</i>, with this bacterium being closely associated with increased spermidine levels. <i>Lactobacillus johnsonii</i> supplementation replicated MBE's hepatoprotection by increasing spermidine and mitigating alcohol-induced hepatic/intestinal damage. Subsequent <i>in vivo</i> and <i>in vitro</i> spermidine interventions further validated its hepatoprotection. These findings propose a novel \"MBE-<i>Lactobacillus johnsonii</i>-spermidine-liver\" regulatory mode, positioning MBE as a dietary or therapeutic candidate for ALD and offering gut-liver axis targets.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The intricate microbial-gut-brain axis in Alzheimer's disease: a review of microbiota-targeted strategies.","authors":"Shu Zhang, Zhenghao Wu, Shuling Zhang, Yuehang Ru, Qinwen Wang, Haoxiang Tong, Qihan Qin, Qingmin Yan, Ziwei Li, Guoqing Wu","doi":"10.1039/d5fo03139g","DOIUrl":"https://doi.org/10.1039/d5fo03139g","url":null,"abstract":"<p><p>The microbiome-gut-brain axis (MGBA) has emerged as a potential focus for the enhancement of cognitive abilities and the improvement of Alzheimer's disease (AD). Probiotics and prebiotics can improve the imbalance of gut microbiota to alleviate AD symptoms. Current research on probiotics/prebiotics and brain function mainly focuses on metabolic pathways such as those involving microbial metabolites like lipopolysaccharides and short-chain fatty acids, as well as immune pathways that regulate inflammation in the gut and brain. However, the roles played by endocrine and neural pathways remain less explored and warrant further attention. This review explores the intricate mechanisms of gut-brain communication within the MGBA, and especially systematically elaborates on the specific mechanisms of the endocrine pathway (impact of gut-derived and exogenous hormones on brain function) and the neural pathway (regulation of brain function by the sympathetic and parasympathetic systems). It also emphasizes the specific changes in gut microbiota noted in individuals with AD. Additionally, it examines the beneficial effects of probiotics, prebiotics, synbiotics, and postbiotics for cognitive function, reviewing their advancements in preclinical research, clinical trials, and commercial applications. Furthermore, this review delves into novel gut microbiota-related strategies to promote brain health, including antibiotics, certain gut-targeted inhibitors or agonists, fecal microbiota transplantation, whole microbiome transplantation, viral microbiota transplantation, genetically engineered bacteria, and bacteriophage-based <i>in situ</i> intestinal microbiome engineering. Ultimately, this review aims to advance the therapeutic application of gut microbiota-targeted strategies in AD.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blackthorn juice from central Italy exhibits selective anticancer activity by inducing apoptosis and autophagy in gastric carcinoma cells.","authors":"Matteo Micucci, Giovanni Caprioli, Ilaria Versari, Laura Acquaticci, Gianni Sagratini, Federico Gianfanti, Francesco Onesimo, Irene Faenza, Sabrina Burattini, Michele Mari, Michele Retini, Riham Osman, Sara Salucci, Michela Battistelli","doi":"10.1039/d5fo02227d","DOIUrl":"https://doi.org/10.1039/d5fo02227d","url":null,"abstract":"<p><p>Blackthorn (<i>Prunus spinosa</i> L.) juice, a polyphenol-rich blend derived from the Marche region of Italy, was chemically characterized and evaluated for its selective effects against gastric cancer cells. The juice contained a mix of polyphenols, including neochlorogenic acid, cyanidin-3-glucoside, rutin, and smaller molecules - such as gallic, caffeic, and vanillic acids - characterized by high absorption rates. <i>In vitro</i>, the juice significantly reduced the viability of AGS and KATO III gastric cancer cells, while sparing non-tumorigenic GES-1 cells. In malignant cells, the juice activated Caspase-3, upregulated Bax, and downregulated Bcl-xL, while also modulating autophagy by increasing LC3B-II levels and decreasing Beclin-1. Mitochondrial impairment was confirmed by cardiolipin loss and cytochrome c release into the cytoplasm. These findings support the potential of <i>Prunus spinosa</i> L. juice as a selective, innovative functional beverage for gastric cancer prevention, acting beyond nutrition but before the need for medical intervention.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healthy dietary patterns in relation to multimorbidity among schoolteachers.","authors":"Jie Shen, Minqing Yan, Yiying Gong, Mengjie He, Liyan Huang, Yimin Zhu, Ting Shen, Dong Zhao, Xiaolin Xu, Ronghua Zhang, Changzheng Yuan","doi":"10.1039/d5fo00333d","DOIUrl":"https://doi.org/10.1039/d5fo00333d","url":null,"abstract":"<p><p><i>Background</i>: Dietary quality has been associated with the development of several chronic diseases. However, few studies have evaluated the association between dietary quality and the co-existence of two or more chronic conditions. <i>Methods</i>: We conducted a cross-sectional analysis among 10 781 schoolteachers (mean age 39.3 ± 9.4 years, 74.4% female) in China. Dietary quality was assessed by five dietary scores, including the Chinese Healthy Eating Index (CHEI), Dietary Approaches to Stop Hypertension (DASH), alternate Mediterranean Diet (aMED), healthful Plant-based Diet Index (hPDI), and Mediterranean-DASH Diet Intervention for Neurodegenerative Delay (MIND) diet. Multimorbidity was defined as the co-existence of two or more chronic conditions, including coronary heart disease, stroke, diabetes, hypertension, hyperlipidemia, cancer, depressive symptoms, and anxiety symptoms. Logistic regression models were used to evaluate the corresponding associations. <i>Results</i>: A total of 1892 (17.5%) participants were classified as having multimorbidity. Compared with participants in the lowest tertile, those in the highest tertile of dietary scores were associated with lower odds of multimorbidity (Odds Ratio [OR] = 0.67, 95% Confidence Interval [CI], 0.59-0.76 for CHEI; OR = 0.76, 95% CI, 0.66-0.87 for aMED; OR = 0.77, 95% CI, 0.68-0.87 for MIND; OR = 0.81, 95% CI, 0.71-0.92 for DASH; OR = 0.86, 95% CI, 0.76-0.98 for hPDI). The associations persisted across major subgroups by age, gender, income level, smoking status, and physical activity. In terms of specific chronic conditions, higher adherence to all five healthy dietary patterns was associated with lower odds of depressive symptoms and anxiety symptoms, whereas CHEI and DASH were associated with lower odds of cardiometabolic diseases. In particular, intake of fresh vegetables was inversely associated with multimorbidity, whereas sugar-sweetened beverages, sodium, and alcohol consumption demonstrated detrimental associations with multimorbidity. <i>Conclusions</i>: Our findings support the potential beneficial role of healthier dietary patterns in maintaining the overall health among Chinese schoolteachers.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction, characterization, and anti-aging evaluation of flavonoids from <i>Citrus reticulata</i> 'Zhangshuensis'.","authors":"Jiapan Gan, Jiayan Xie, Xin Xu, Jiahao Ai, Sisi He, Jiafen Zou, Qiang Yu, Hongli Jin, Yanfang Liu, Xinmiao Liang","doi":"10.1039/d5fo02218e","DOIUrl":"https://doi.org/10.1039/d5fo02218e","url":null,"abstract":"<p><p>ZhangTouHong (ZTH), a highly valuable edible-medicinal resource rich in bioactive flavonoids, has little been studied in terms of its chemical composition and biological activities. In this work, a process was developed for the preparation of flavonoid multi-components (FMCs) from ZTH, which showed high efficiency and specificity and a 4.62-fold increased total flavonoid content. Based on UPLC-Q-TOF-MS/MS and molecular networks, the FMCs in ZTH were identified for the first time, with a total of 48 flavonoid compounds, including 3 potential new compounds. Moreover, the results of anti-aging activity tests demonstrated that the FMCs have excellent antioxidant activity and anti-aging capacity, extending the lifespan of nematodes by 21.65% and the survival time of oxidative stress in nematodes by 35.71%. In summary, this study established an efficient and scalable process for the preparation of bioactive flavonoid components and, for the first time, verified the anti-aging effect of ZTH FMCs, providing the scientific basis for the deep development and industrial application of this characteristic citrus resource.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of glycolysis alleviates food allergy <i>via</i> regulating the immune response of T helper cells and mast cells to ovalbumin in sensitized mice.","authors":"Fangfang Min, Huming Shao, Zhongliang Wang, Qiang Shi, Ping Tong, Jingjing Li, Meini Wang, Xin Li, Yong Wu, Hongbing Chen","doi":"10.1039/d5fo00637f","DOIUrl":"https://doi.org/10.1039/d5fo00637f","url":null,"abstract":"<p><p>Ingestion of food allergens can elicit metabolic alterations in the host during the induction of allergic responses. Gaining a grasp of the key events underlying these changes will contribute to a better understanding of the mechanism of food allergy. In this study, we aimed to investigate the role of glycolysis, an important metabolic pathway, in the development of food allergy (FA). The glycolysis inhibitor 2-deoxy-D-glucose (2-DG) was utilized in an ovalbumin (OVA)-induced FA mouse model prior to oral allergen challenges, and the results found that inhibition of glycolysis effectively alleviated allergic diarrhea (2-DG group: 0% <i>vs.</i> FA group: 100%) and significantly decreased allergen-specific IgE levels. Further analysis revealed the inhibiting effect of 2-DG intervention on the systemic and intestinal Th2 immune responses, mainly manifested by suppressed Th2 cell differentiation and IL-4 expression. Additionally, blockade of glycolysis was shown to decrease intestinal mast cell accumulation, serum mast cell protease-1 levels, and the expression of typical mast cell markers (<i>e.g.</i>, <i>Fcer1a</i>, <i>Mcpt1</i>, and <i>Il1rl1</i>). Moreover, <i>in vitro</i> experiments indicated that blockade of glycolysis by 2-DG dampened early receptor signaling in mast cell activation and its function. In summary, these results demonstrate that the glycolytic metabolic pathway was deeply involved in FA, and that the alleviation of allergic reactions by glycolysis inhibition was achieved <i>via</i> modulating the synergistic immune response of Th2 cells and mast cells to ingested allergens.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}