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The effect of molecular weight on the gut microbiota modulation and hypoglycemic effect in vivo of Blackberry polysaccharides 分子量对黑莓多糖体内肠道微生物群调节和降血糖作用的影响
IF 6.1 1区 农林科学
Food & Function Pub Date : 2024-07-11 DOI: 10.1039/d4fo01989j
Xi Lai, Bing Wei Xu, Shuyong Fu, Hua Sheng Li, Xiong Fu, Chin Ping Tan, Pingping Wang, Zuman Dou, Chun Chen
{"title":"The effect of molecular weight on the gut microbiota modulation and hypoglycemic effect in vivo of Blackberry polysaccharides","authors":"Xi Lai, Bing Wei Xu, Shuyong Fu, Hua Sheng Li, Xiong Fu, Chin Ping Tan, Pingping Wang, Zuman Dou, Chun Chen","doi":"10.1039/d4fo01989j","DOIUrl":"https://doi.org/10.1039/d4fo01989j","url":null,"abstract":"Blackberry polysaccharides with certain molecular weight distribution have good bioactivity. In this research, the type 2 diabetes mice was used to investigate the hypoglycemic effect of Blackberry polysaccharides with three different molecular weight that BBP (603.59KDa), BBP-8 (408.13KDa) and BBP-24 (247.62KDa) through gut microbiota modulation. Blackberry polysaccharides exhibited stronger hypoglycemic activity after degradation that the FBG of BBP, BBP-8 and BBP-24 was reduced to 20.21±4.17 mmol/L, 20.6±7.23 mmol/L and 17.32±6.59 mmol/L as well as OGTT-AUC was reduced by 14.76%, 19.80% and 25.04% after 8 weeks intervention. Furthermore, the 16S rRNA gene sequencing analysis indicated that BBP, BBP-8 and BBP-24 could up-regulate the metabolic product SCFAs (acetic acid, propionic acid, and n-butyric acid) and reshape the diversity and composition of the gut microbiota. From 0 to 4 weeks, the F/B of BBP, BBP-8 and BBP-24 reduced by 56.44%, 47.19% and 62.04%, as well as reaching 3.39, 6.54, and 3.11 in the 8th week, which suggested the faster utilization of BBP-24. Moreover, three Blackberry polysaccharides intervention increased the relative abundance of targeted beneficial bacteria Oscillospira and Bacteroidaceae_Bacteroides and decreased the relative abundance of pathogenic bacteria Allobaculum. In general, the result demonstrated the blackberry polysaccharides with lower molecular weight are more easily to be fermented which made the theoretical basis for the development of Blackberry polysaccharides as probiotic food to rapidly regulate intestinal flora for type 2 diabetes.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141586200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Treatment with walnut peptide ameliorates memory impairment in zebrafish and rats: promoting the expression of neurotrophic factors and suppressing oxidative stress. 核桃肽能改善斑马鱼和大鼠的记忆损伤:促进神经营养因子的表达并抑制氧化应激。
IF 5.1 1区 农林科学
Food & Function Pub Date : 2024-07-11 DOI: 10.1039/d4fo00074a
Wei Wei, Qiming Wu, Shuai Wang, Chuanmin Dong, Shujuan Shao, Zhao Zhang, Xiping Zhang, Xuejun Zhang, Juntao Kan, Fuguo Liu
{"title":"Treatment with walnut peptide ameliorates memory impairment in zebrafish and rats: promoting the expression of neurotrophic factors and suppressing oxidative stress.","authors":"Wei Wei, Qiming Wu, Shuai Wang, Chuanmin Dong, Shujuan Shao, Zhao Zhang, Xiping Zhang, Xuejun Zhang, Juntao Kan, Fuguo Liu","doi":"10.1039/d4fo00074a","DOIUrl":"https://doi.org/10.1039/d4fo00074a","url":null,"abstract":"<p><p>Walnut peptide, a low molecular weight peptide separated from walnuts by enzymatic hydrolysis, is considered as a potential nutraceutical with a variety of biological activities. In this study, we characterized the walnut peptide prepared by alkaline protease hydrolysis and evaluated its neuroprotective effect in zebrafish and rat models of memory disorders. Series of concentrations of the walnut peptide were orally administered to zebrafish and rats to examine its impact on the behavior and biochemical indicators. The results showed that the oral administration of walnut peptide significantly ameliorated the behavioral performance in zebrafish exposed to bisphenol AF (1 μg mL<sup>-1</sup>) and rats exposed to alcohol (30% ethanol, 10 mL kg<sup>-1</sup>). Furthermore, the walnut peptide upregulated the expression of neurotrophic-related molecules in zebrafish, such as the brain-derived neurotrophic factor (BDNF) and the glial cell-derived neurotrophic factor (GDNF). In the rat brain, the walnut peptide increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), while dramatically reduced malondialdehyde (MDA) level. Together, these findings elucidated that the walnut peptide might partially offset the declarative memory deficits <i>via</i> regulation of neurotrophic-related molecule expression and promotion of the antioxidant defense ability. Therefore, walnut peptide holds the potential for development into functional foods as a nutritional supplement for the management of certain neurodegenerative disorders.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aloe polysaccharides ameliorate obesity-associated cognitive dysfunction in high-fat diet-fed mice by targeting the gut microbiota and intestinal barrier integrity. 芦荟多糖通过靶向肠道微生物群和肠屏障完整性,改善高脂饮食喂养小鼠肥胖相关的认知功能障碍。
IF 5.1 1区 农林科学
Food & Function Pub Date : 2024-07-11 DOI: 10.1039/d4fo01844c
Ling Mo, Jingjing Li, Hangsun Lu, Shaoda Lu, Henghui Fu, Bo Huang, Chaochao Zhao
{"title":"Aloe polysaccharides ameliorate obesity-associated cognitive dysfunction in high-fat diet-fed mice by targeting the gut microbiota and intestinal barrier integrity.","authors":"Ling Mo, Jingjing Li, Hangsun Lu, Shaoda Lu, Henghui Fu, Bo Huang, Chaochao Zhao","doi":"10.1039/d4fo01844c","DOIUrl":"https://doi.org/10.1039/d4fo01844c","url":null,"abstract":"<p><p>Aloe polysaccharides (APs) display cognition-improving properties, but the underlying mechanisms remain unclear. Herein, AP supplementation for 24 weeks significantly improved cognitive behavioral disturbances caused by a high-fat diet. Moreover, APs notably reshaped the structure of the gut microbiota, which was manifested by increasing the relative abundance of <i>Alloprevotella</i>, <i>Alistipes</i>, <i>Romboutsia</i>, <i>Turicibacter</i>, <i>Prevotellaceae_UCG-001</i>, and <i>Akkermansia</i> while reducing the abundance of <i>Parasutterella</i>, <i>Staphylococcus</i>, <i>Helicobacter</i>, <i>Enterococcus</i>, and <i>Erysipelatoclostridium</i>. Notably, the gut barrier damage and LPS leakage caused by HF were recovered by APs. Additionally, with the improvement of intestinal barrier integrity, oxidative stress and inflammation in the brain and jejunum were significantly ameliorated. Furthermore, the expression of genes associated with cognitive impairment and the intestinal tract barrier was up-regulated (<i>CREB, BDNF</i>, <i>TrkB</i>, <i>ZO-1</i> and <i>occludin</i>), while the expression of genes associated with inflammatory factors was down-regulated (<i>IL-1β</i>, <i>IL-6</i>, and <i>TNF-α</i>). Finally, we observed a significant correlation among cognition-related genes, gut microbiota, oxidative stress, and inflammation in the HF-AP group. Together, our findings suggest that altered gut microbiota composition and improved gut barrier integrity may be important targets for potentially improving high-fat diet-induced cognitive impairment.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Digested casein phosphopeptides impact intestinal calcium transport in vitro 消化酪蛋白磷酸肽对体外肠钙转运的影响
IF 6.1 1区 农林科学
Food & Function Pub Date : 2024-07-11 DOI: 10.1039/d4fo01637h
Mathie Tenenbaum, Barbara Deracinois, Camille Dugardin, Julie Auger, Alain Baniel, Audrey Boulier, Christophe Flahaut, Rozenn Ravallec, Benoit Cudennec
{"title":"Digested casein phosphopeptides impact intestinal calcium transport in vitro","authors":"Mathie Tenenbaum, Barbara Deracinois, Camille Dugardin, Julie Auger, Alain Baniel, Audrey Boulier, Christophe Flahaut, Rozenn Ravallec, Benoit Cudennec","doi":"10.1039/d4fo01637h","DOIUrl":"https://doi.org/10.1039/d4fo01637h","url":null,"abstract":"Calcium is the most abundant mineral in the human body and is involved in critical physiological and cellular processes. It is essential for the development, maintenance, and integrity of bone tissue throughout life. Identifying new natural food-grade chelating agents to improve calcium uptake is of increasing interest. Casein phosphopeptides (CPPs), highly phosphorylated peptides obtained after enzymatic hydrolysis of caseins, represent promising calcium-chelating candidates. The aim of this study was to investigate, using cell culture models, the ability of a digested milk matrix enriched in CPPs to regulate calcium transport through the intestinal barrier and elucidate the involved mechanisms. To this end, a CPP-preparation underwent in vitro static digestion and was subsequently incubated with an intestinal barrier model to monitor calcium uptake and transport. Our results demonstrated that the digested CPP preparation enhanced the trans-epithelial calcium transport via paracellular pathways and that CPPs, identified by peptidomics, crossed the intestinal barrier in the same time.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141586201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The particle sizes of milled wheat fractions affect the in vitro starch digestibility and quality parameters of wire-cut cookies made thereof. 碾碎的小麦馏分的粒度会影响体外淀粉消化率及其制成的线切割饼干的质量参数。
IF 5.1 1区 农林科学
Food & Function Pub Date : 2024-07-10 DOI: 10.1039/d4fo01315h
Leonardo I Mulargia, Elien Lemmens, Kurt Gebruers, Pierre D Udekem D Acoz, Arno G B Wouters, Jan A Delcour
{"title":"The particle sizes of milled wheat fractions affect the <i>in vitro</i> starch digestibility and quality parameters of wire-cut cookies made thereof.","authors":"Leonardo I Mulargia, Elien Lemmens, Kurt Gebruers, Pierre D Udekem D Acoz, Arno G B Wouters, Jan A Delcour","doi":"10.1039/d4fo01315h","DOIUrl":"https://doi.org/10.1039/d4fo01315h","url":null,"abstract":"<p><p>Slow digestion of starch is linked to various health benefits. The impact of wheat particle size on <i>in vitro</i> starch digestibility and quality of wire-cut cookies was here evaluated by including four soft wheat fractions [<i>i.e.</i> flour (average diameter, 83 μm), fine farina (643 μm), coarse farina (999 μm) and bran (1036 μm)] in the recipe. The susceptibility of starch in these fractions to <i>in vitro</i> digestion decreased with increasing particle size, resulting in a 76% lower digestion rate for coarse farina than for flour as found with the single first-order kinetic model. Starch was protected from hydrolysis likely due to delayed diffusion of pancreatic α-amylase through the intact farina cell walls. When 20-65% starch in flour for the control cookie recipe was substituted with the same percentages in fine and coarse farina, the starch digestion rate decreased when substitution levels increased. A 62% lower digestion rate was found at 65% substitution with coarse farina. Cell wall intactness was largely preserved in the cookies and most of the starch appeared as ungelatinised granules. Further, the cookie spread ratio during baking was 48% and 33% higher and the cookies were 63% and 57% less hard than control cookies when made with 65% fine farina and 65% coarse farina, respectively. The relatively low specific surface area of large wheat particles resulted in low water absorption and less dense packing. In conclusion, encapsulation of starch by intact cell walls in coarse wheat fractions makes them promising ingredients when developing starchy food products for controlled energy release.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bifidobacterium bifidum alleviates adenine-induced acute kidney injury in mice by improving intestinal barrier function. 双歧杆菌通过改善肠道屏障功能减轻腺嘌呤诱导的小鼠急性肾损伤
IF 5.1 1区 农林科学
Food & Function Pub Date : 2024-07-10 DOI: 10.1039/d4fo02014f
Yang Meng, Maozhen Zhao, Qiyu Ma, Qinglian Hua, Jinpeng Hu, Qi Zhou, Huaxi Yi, Zhe Zhang, Lanwei Zhang
{"title":"<i>Bifidobacterium bifidum</i> alleviates adenine-induced acute kidney injury in mice by improving intestinal barrier function.","authors":"Yang Meng, Maozhen Zhao, Qiyu Ma, Qinglian Hua, Jinpeng Hu, Qi Zhou, Huaxi Yi, Zhe Zhang, Lanwei Zhang","doi":"10.1039/d4fo02014f","DOIUrl":"https://doi.org/10.1039/d4fo02014f","url":null,"abstract":"<p><p>Acute kidney injury (AKI) is a kind of critical kidney disease characterized by tubular injury, rapid decline of renal function and renal inflammation, with high clinical incidence. AKI has been shown to be associated with dysregulation of the gut microbiota and impaired intestinal barrier. <i>Bifidobacterium</i> has a positive impact on the treatment of many diseases. However, little is known about the role and mechanism of <i>Bifidobacterium</i> in AKI. Based on previous experiments, <i>Bifidobacterium bifidum</i> FL228.1 and FL276.1, which can relieve intestinal inflammation, and <i>Bifidobacterium bifidum</i> ZL.1, which has anti-inflammatory potential, were screened. This study aimed to investigate the effects of <i>Bifidobacterium bifidum</i> FL228.1, FL276.1 and ZL.1 on AKI, focusing on their role in the gut microbiota composition and intestinal barrier function. Our results showed that <i>Bifidobacterium bifidum</i> FL228.1, FL276.1 and ZL.1 effectively improved kidney function in mice with AKI by regulating the gut microbiota dysregulation, inhibiting intestinal inflammation and rebuilding the intestinal mucosal barrier. In addition, intervention with probiotics turned the gut microbiota disturbance caused by AKI into a normalized trend, reversed the adverse outcome of microbiota imbalance, and increased the abundance of potentially beneficial bacteria <i>Bifidobacterium</i> and <i>Faecalibaculum</i>. In summary, <i>Bifidobacterium bifidum</i> FL228.1, FL276.1, and ZL.1 alleviate adenine-induced AKI based on the gut-kidney axis. Although their mechanisms of action are different, their effect on alleviating AKI is almost the same.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selenium supplementation via modulation of selenoproteins ameliorates binge drinking-induced oxidative, energetic, metabolic, and endocrine imbalance in adolescent rats' skeletal muscle. 通过调节硒蛋白补充硒可改善暴饮引起的青少年大鼠骨骼肌氧化、能量、代谢和内分泌失衡。
IF 5.1 1区 农林科学
Food & Function Pub Date : 2024-07-10 DOI: 10.1039/d4fo01354a
Inés Romero-Herrera, Fátima Nogales, María Del Carmen Gallego-López, Javier Díaz-Castro, Olimpia Carreras, María Luisa Ojeda
{"title":"Selenium supplementation <i>via</i> modulation of selenoproteins ameliorates binge drinking-induced oxidative, energetic, metabolic, and endocrine imbalance in adolescent rats' skeletal muscle.","authors":"Inés Romero-Herrera, Fátima Nogales, María Del Carmen Gallego-López, Javier Díaz-Castro, Olimpia Carreras, María Luisa Ojeda","doi":"10.1039/d4fo01354a","DOIUrl":"https://doi.org/10.1039/d4fo01354a","url":null,"abstract":"<p><p>Adolescence is characterized by increased vulnerability to addiction and ethanol (EtOH) toxicity, particularly through binge drinking (BD), a favored acute EtOH-ingestion pattern among teenagers. BD, highly pro-oxidant, induces oxidative stress (OS), affecting skeletal muscle (SKM), where selenium (Se), an antioxidant element and catalytic center of selenoproteins, is stored, among other tissues. Investigating the effects of Se supplementation on SKM after BD exposure holds therapeutic promise. For this, we randomised 32 adolescent Wistar rats into 4 groups, exposed or not to intermittent i.p. BD [BD and control (C)] (3 g EtOH per kg per day), and supplemented with selenite [BDSe and CSe] (0.4 ppm). In SKM, we examined the oxidative balance, energy status (AMPK, SIRT-1), protein turnover (IRS-1, Akt1, mTOR, IGF-1, NF-κB p65, MAFbx, ULK1, pelF2α), serum myokines (myostatin, IL-6, FGF21, irisin, BDNF, IL-15, fractalkine, FSTL-1, FABP-3), and selenoproteins (GPx1, GPx4, SelM, SelP). In the pancreas, we studied the oxidative balance and SIRT-1 expression. Selenite supplementation mitigated BD-induced OS by enhancing the expression of selenoproteins, which restored oxidative balance, notably stimulating protein synthesis and normalizing the myokine profile, leading to improved SKM mass growth and metabolism, and reduced inflammation and apoptosis (caspase-3). Selenite restoration of SelP's receptor LRP1 expression, reduced by BD, outlines the crucial role of SKM in the SelP cycle, linking Se levels to SKM development. Furthermore, Se attenuated pancreatic OS, preserving insulin secretion. Se supplementation shows potential for alleviating SKM damage from BD, with additional beneficial endocrine effects on the pancreas, adipose tissue, liver, heart and brain that position it as a broad-spectrum treatment for adolescent alcohol consumption, preventing metabolic diseases in adulthood.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potential bioaccessibility and bioavailability of polyphenols and functional properties of tiger nut beverage and by-product during in vitro digestion 虎坚果饮料和副产品在体外消化过程中多酚的潜在生物可及性和生物利用率以及功能特性
IF 6.1 1区 农林科学
Food & Function Pub Date : 2024-07-05 DOI: 10.1039/d4fo01537a
Paula Llorens, Manuela Flavia Chiacchio, Silvia Tagliamonte, Ana Juan-García, Noelia Pallarés, Juan Carlos Moltó, Paola Vitaglione, Cristina Juan
{"title":"Potential bioaccessibility and bioavailability of polyphenols and functional properties of tiger nut beverage and by-product during in vitro digestion","authors":"Paula Llorens, Manuela Flavia Chiacchio, Silvia Tagliamonte, Ana Juan-García, Noelia Pallarés, Juan Carlos Moltó, Paola Vitaglione, Cristina Juan","doi":"10.1039/d4fo01537a","DOIUrl":"https://doi.org/10.1039/d4fo01537a","url":null,"abstract":"“Horchata de chufa”is a beverage produced from tiger nut tubers which yields high amount of by-product. This study explored the functional properties of the Spanish tiger nut beverage (TNB) and its by-product (TNBP) along the bioaccessibility and bioavailability of the polyphenols in vitro. TNB and TNBP were characterized for polyphenols by LC/MS/MS and underwent in vitro digestion (INFOGEST). Total antioxidant capacity (TAC) in all bioaccessible fractions and digestion residues was assessed. The intestinal bioaccessible fractions were tested for the ability to inhibit the activity of digestive enzymes (α-amylase, α-glucosidase, and lipase), and content of polyphenols, whose bioavailability was assessed in a Caco-2 cell model. Thirteen polyphenols were quantified and found to be more abundant in TNB (603±1.4 µg/g DW) than TNBP (187±1.0 µg/g DW). Polyphenol bioaccessibility was higher from TNBP than TNB (57% vs 27%) and despite a similar TAC of the intestinal bioaccessible fractions (10.2±0.1 µmoL vs 9.2±0.03 µmoL eq.Trolox/g DW for TNB and TNBP, respectively), the different pattern of polyphenols released upon digestion possibly determined the higher ability of TNBP fraction to inhibit α-glucosidase and of TNB towards lipase. The TNBP digestion residue showed higher TAC than TNB. Moreover, TNB polyphenols exhibited over 80% bioavailability, while TNBP's ranged from 62% to 84%. Overall, the findings demonstrated that TNBP maintains a high nutritional value thus suggesting its possible reuse in innovative, healthy, and sustainable foods.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141575041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification, screening and molecular mechanisms of natural stable angiotensin-converting enzyme (ACE) inhibitory peptides from foxtail millet protein hydrolysates: a combined in silico and in vitro study. 从狐尾粟蛋白水解物中鉴定、筛选天然稳定的血管紧张素转换酶(ACE)抑制肽及其分子机制:一项硅学和体外联合研究。
IF 5.1 1区 农林科学
Food & Function Pub Date : 2024-07-05 DOI: 10.1039/d4fo01992j
Yiqing Zhu, Changyu Chen, Zijian Dai, Han Wang, Yiyun Zhang, Qingyu Zhao, Yong Xue, Qun Shen
{"title":"Identification, screening and molecular mechanisms of natural stable angiotensin-converting enzyme (ACE) inhibitory peptides from foxtail millet protein hydrolysates: a combined <i>in silico</i> and <i>in vitro</i> study.","authors":"Yiqing Zhu, Changyu Chen, Zijian Dai, Han Wang, Yiyun Zhang, Qingyu Zhao, Yong Xue, Qun Shen","doi":"10.1039/d4fo01992j","DOIUrl":"https://doi.org/10.1039/d4fo01992j","url":null,"abstract":"<p><p>The stability of bioactive peptides under various food processing conditions is the basis for their use in industrial manufacturing. This study aimed to identify natural ACE inhibitors with excellent stability and investigate their physicochemical properties and putative molecular mechanisms. Five novel ACE inhibitory peptides (QDPLFPL, FPGVSPF, SPAQLLPF, LVPYRP, and WYWPQ) were isolated and identified using RP-HPLC and Nano LC-MS/MS with foxtail millet protein hydrolysates as the raw material. These peptides are non-toxic and exhibit strong ACE inhibitory activity <i>in vitro</i> (IC<sub>50</sub> values between 0.13 mg mL<sup>-1</sup> and 0.56 mg mL<sup>-1</sup>). In addition to QDPLFPL, FPGVSPF, SPAQLLPF, LVPYRP, and WYWPQ have excellent human intestinal absorption. Compared to FPGVSPF and SPAQLLPF, the stable helical structure of LVPYRP and WYWPQ allows them to maintain high stability under conditions that mimic gastrointestinal digestion and various food processing (temperatures, pH, sucrose, NaCl, citric acid, sodium benzoate, Cu<sup>2+</sup>, Zn<sup>2+</sup>, K<sup>+</sup>, Mg<sup>2+</sup>, Ca<sup>2+</sup>). The results of molecular docking and molecular dynamics simulation suggest that LVPYRP has greater stability and binding capacity to ACE than WYWPQ. LVPYRP might attach to the active pockets (S1, S2, and S1') of ACE <i>via</i> hydrogen bonds and hydrophobic interactions, then compete with Zn<sup>2+</sup> in ACE to demonstrate its ACE inhibitory activity. The binding of LVPYRP to ACE enhances the rearrangement of ACE's active structural domains, with electrostatic and polar solvation energy contributing the most energy to the binding. Our findings suggested that LVPYRP derived from foxtail millet protein hydrolysates has the potential to be incorporated into functional foods to provide antihypertensive benefits.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141532844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Circulating low-molecular-weight (poly)phenol metabolites in the brain: unveiling in vitro and in vivo blood-brain barrier transport. 大脑中循环的低分子量(多)苯酚代谢物:揭示体外和体内血脑屏障的转运。
IF 5.1 1区 农林科学
Food & Function Pub Date : 2024-07-05 DOI: 10.1039/d4fo01396d
Rafael Carecho, Daniela Marques, Diogo Carregosa, Domenico Masuero, Mar Garcia-Aloy, Federica Tramer, Sabina Passamonti, Urska Vrhovsek, M Rita Ventura, Maria Alexandra Brito, Cláudia Nunes Dos Santos, Inês Figueira
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