Food & Function最新文献

{"title":"Ergosterol from edible fungi: Enhancing fatty acid oxidation <i>via</i> CPT1A to protect against diabetic kidney disease.","authors":"Zhonghua Dong, Xiao Li, Xuan Wang, Jingya Xu, Wei Xu","doi":"10.1039/d5fo00371g","DOIUrl":"https://doi.org/10.1039/d5fo00371g","url":null,"abstract":"<p><p><i>Background</i>: Diabetic kidney disease (DKD), a common microvascular complication of diabetes mellitus, is recognized as a leading cause of end-stage renal disease. Ergosterol, a natural sterol abundant in edible fungi, has shown pharmacological effects that may benefit DKD treatment. However, its precise mechanisms of action remain elusive. This study aimed to evaluate the therapeutic efficacy of ergosterol in DKD and to delineate the underlying mechanisms. <i>Methods</i>: Transcriptome microarray sequencing data from DKD patients retrieved from the public GEO database, as well as data from mouse DKD models, were analyzed to identify differentially expressed genes. Db/db mouse, high-glucose-induced HK-2 cells and conditioned THP-1 cells were employed to evaluate the impact of ergosterol on renal function, lipid metabolism, and macrophage phenotypic transformation. <i>Results</i>: Transcriptional profiling of DKD kidneys revealed alterations in fatty acid metabolism, which were corroborated in db/db mice. Ergosterol significantly improved renal function, reduced lipid accumulation, and mitigated inflammation. CPT1A, a key modulator of fatty acid metabolism, was identified as a target. The inhibition of CPT1A in renal tubular epithelial cells led to impaired fatty acid oxidation and lipid accumulation. Excessive renal lipids further stimulated macrophages to transform into pro-inflammatory phenotypes, leading to renal inflammation infiltration and exacerbating kidney damage. Ergosterol upregulated CPT1A expression through transcriptional regulation of FOXA1, thereby reducing lipid accumulation and subsequent renal inflammation. <i>Conclusion</i>: Ergosterol enhances renal fatty acid oxidation <i>via</i> the FOXA1/CPT1A pathway, reducing renal lipid accumulation and inflammation, potentially delaying DKD progression. This study elucidates the therapeutic potential of ergosterol in DKD therapy and provides new insights into the treatment of this disease.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased protein intake in healthy males exposed to an appetite modulating, whey-derived peptide hydrolysate.","authors":"Erin Stella Sullivan, Harriët Schellekens, Brendan T Griffin, Samantha J Cushen, Ken Howick, John F Cryan, Darren Dahly, Nessa Noronha, Aoife M Ryan","doi":"10.1039/d4fo06083k","DOIUrl":"https://doi.org/10.1039/d4fo06083k","url":null,"abstract":"<p><p>The identification of food-grade bioactives with proven orexigenic effects would mark significant progress in the treatment of disease-related malnutrition. To investigate the effects of two milk-derived hydrolysates (UL-2-141 (whey hydrolysate) and MF1145 (casein hydrolysate)) on appetite and energy intake in healthy humans, a single-blind, placebo-controlled, 3-arm cross-over feeding trial was conducted in 22 fasted, cannulated healthy male volunteers. Participants received 26 mg kg^-1 of both hydrolysates and placebo and were observed from morning to afternoon with a set breakfast and <i>ad libitum</i> lunch. Mean total daily energy and protein intakes when treated with placebo were 2673 kcal (95% CI: 2247-3100 kcal) and 128 g (95% CI: 105-152 g), respectively. Energy intake for either treatment was not significantly different from that for placebo (<i>p</i> = 0.266 for UL-2-141 and <i>p</i> = 0.796 for MF1145). Protein intake significantly increased in the UL-2-141 arm compared with that in placebo (+23 g, <i>p</i> = 0.044), but it did not significantly increase in the MF1145 arm (+13 g, <i>p</i> = 0.189). Appetite, hunger and satiety responses on VAS for either treatment were not significantly different from those obtained for placebo. GLP-1 was significantly higher pre-lunch in the UL-2-141 arm than in placebo (+8 pmol L^-1, <i>p</i> = 0.01) and in the MF1145 arm (+7 pmol L^-1, <i>p</i> = 0.039). GH was significantly lower pre-lunch only in the UL-2-141 arm than in placebo (-133 pg mL^-1, <i>p</i> = 0.027). Protein intake was significantly increased in the UL-2-141 arm, demonstrating appetite modulation, potentially <i>via</i> indirect or delayed stimulation of the ghrelin receptor. Since healthy adults are often not in tune with their physiological hunger, repeating the study in subjects with established anorexia may be prudent.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergy of secoisolariciresinol diglucoside and fructooligosaccharide in enhancement of the intestinal mucus barrier by relieving ER stress and regulating the gut microbiome.","authors":"Li Zhang, Muhua Ji, Ning Li, Jingjing Yu, Guiming Yan, Yan Di, Yuchun Zhang, Qian Liu, Lijun Sun, Xuebo Liu, Yutang Wang","doi":"10.1039/d5fo01526j","DOIUrl":"https://doi.org/10.1039/d5fo01526j","url":null,"abstract":"<p><p>The impairment of the intestinal barrier and the high incidence of gastrointestinal diseases can be caused by poor dietary patterns, such as long-term high-fat diets. The mucous layer, composed of mucin-2-based glycoproteins, is important for the intestinal barrier. Mucins are secreted by the goblet cells (GC), which are highly sensitive to endoplasmic reticulum (ER) stress. Therefore, promoting mucin synthesis and secretion by relieving ER stress is an effective way to enhance the intestinal mucus barrier. On the other hand, mucins are influenced by the gut microbiome. Mucin-associated bacteria can degrade mucins and then stimulate goblet cells to secrete mucin. Secoisolariciresinol diglucoside (SDG) has been reported to relieve ER stress and improve the gut barrier. As a prebiotic, fructooligosaccharide (FOS) has the potential to regulate intestinal flora. In the present study, we investigated the synergistic effect of SDG and FOS on the improvement of the intestinal mucus barrier in high-fat mice. It was found that both SDG and FOS alleviated histological damage in the colon, inhibited mRNA expression of proinflammatory cytokines, and improved the mucosal barrier. Notably, the synergistic treatment exhibited a better effect. Goblet cell differentiation was promoted, consistent with the relief of ER stress, and SDG might be superior in this process. FOS primarily regulated the gut microbiota, enriched mucus-associated bacteria, and increased the content of metabolite short-chain fatty acids (SCFAs). The simultaneous treatment displayed a synergistic effect. The results indicated that the synergistic treatment had a stronger effect on enhancing the intestinal mucus barrier from dual approaches of relieving ER stress and regulating gut microbiota. This study provides a novel viewpoint and strategy for improving intestinal health.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprocessed black rice bran protects mice against alcohol-induced fatty liver formation.","authors":"Ki Sun Kwon, Su A In, Wha Young Lee, Jeanman Kim, Sang Jong Lee, Sung Phil Kim, Mendel Friedman","doi":"10.1039/d5fo01151e","DOIUrl":"https://doi.org/10.1039/d5fo01151e","url":null,"abstract":"<p><p>Following consumption, alcohol is absorbed from the digestive tract into the circulation and is then distributed into different organs. In the liver, it causes a range of injuries beginning with the induction of fatty liver (hepatic steatosis), which on further alcohol consumption progresses to liver cirrhosis that can result in mortality. On the basis of a series of our previous published studies that showed that black rice-based functional foods have multi-functional health benefits in rodents, including protection of mice and rats against inflammation and alcohol-induced hangovers, we were motivated to determine the protective effect of a polysaccharide-containing bioprocessed (fermented) black rice bran product (BRB-F) to prevent alcohol-induced liver damage. BRB-F prevented dietary alcohol-induced increases in both whole liver and in liver fat weights, serum levels of hepatic enzymes and bilirubin, oxidized liver glutathione, and proinflammatory serum and adipocyte cytokines. Silymarin, a plant phenolic anti-inflammatory agent, cooperated with BRB-F to increase the prevention of alcohol-induced liver damage. BRB-F and silymarin also prevented direct toxicity of HepG2 human liver cells in culture by reducing reactive oxygen species. Mechanistic aspects and suggestions for further research are discussed. The ability of BRB-F alone or with silymarin to protect mice against alcohol-induced liver damage merits confirmation in human clinical studies.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct interaction between <i>Lactiplantibacillus plantarum</i> ZJ316-derived lipoteichoic acid and TLR2 mediates anti-inflammatory and barrier-protective effects in intestinal cells.","authors":"Jiarun Han, Lina Ding, Xin Zhao, Jiaqi Liu, Jiaqi Yin, Qi Wang, Ping Li, Qing Gu","doi":"10.1039/d5fo01925g","DOIUrl":"https://doi.org/10.1039/d5fo01925g","url":null,"abstract":"<p><p>Lipoteichoic acid (LTA), a key bioactive substance of the Gram-positive bacterial cell wall, has garnered attention for its immunomodulatory properties. Herein, we investigated the underlying molecular mechanism by which LTA derived from <i>Lactiplantibacillus plantarum</i> ZJ316 exerts anti-inflammatory effects through interaction with Toll-like receptor 2 (TLR2). Molecular docking, dynamics simulations, and surface plasmon resonance (SPR) indicated a strong and specific binding affinity (<i>K</i>_D = 1.02 μM), with key residues (<i>e.g.</i>, Lys422, Arg486, Arg508) involved in stabilizing the LTA-TLR2 complex. Using an <i>in vitro</i> inflammatory model of Caco-2 cells induced by macrophage supernatant, we demonstrated that LTA significantly upregulated TLR2 expression and inhibited the ERK and p38 MAPK phosphorylation, resulting in reduced secretion of pro-inflammatory cytokines (TNF-α, IL-8) and enhanced anti-inflammatory IL-10 expression. Furthermore, LTA protected intestinal epithelial barrier function by enhancing the expression of tight junction proteins (ZO-1, Occludin, and Claudin-1). These findings highlight the potential of <i>L. plantarum</i> ZJ316-derived LTA as a bioactive component for intestinal health and provide new insight into its regulatory mechanism <i>via</i> the TLR2-MAPK signaling pathway.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary total, animal, and plant protein-energy ratio and risk of mortality: results from the NHANES III and a lifelong animal experiment.","authors":"Zican Li, Lianjie Huang, Qiushuang Zhu, Fanyun Wang, Defang Li, Bo Qu, Runan Zhang, Yue Guan, Akinkunmi Paul Okekunl, Jiaying Yu, Yuqing Wu, Xuanfeng Tang, Xiaoqing Li, Xinyi Lv, Xiang Shu, Xiangju Kong, Rennan Feng","doi":"10.1039/d4fo05785f","DOIUrl":"https://doi.org/10.1039/d4fo05785f","url":null,"abstract":"<p><p><i>Background</i>: Protein is essential for basic physiological functions of the body, but the relationship between excessive protein intake and health is controversial. The association between dietary protein and mortality may depend on protein intake and source. This study examined the links between the total/animal/plant protein-energy ratios and mortality from all causes, cardiovascular disease (CVD) and cancer. <i>Methods</i>: The population study included 13 490 participants with a median follow-up of 26.5 years from NHANES III. The study examined the relationship between total, animal, and plant protein-energy ratios, and their combinations, with mortality through Cox proportional hazards models, restricted cubic splines (RCS), and isocaloric (2.5%, 5%, and 10%) substitution analysis. In an animal experiment, 60 male Wistar rats were randomly assigned to normal protein diets (NC = 30) or isocaloric high-protein (HP) diets (HP = 30) for 101 weeks. Subsequently, renal histologic staining was performed using hematoxylin and eosin, and serum amino acid levels were measured through targeted metabolomics. Changes in hepatic gene expression profiles were assessed <i>via</i> principal component analysis (PCA), analyzed using protein interaction networks and modules with the STRING online database, and validated through quantitative real-time PCR (q-PCR). <i>Results</i>: Total protein-energy ratio was positively associated with all-cause mortality (<i>p</i>-trend = 0.003), and animal-protein-energy ratio was also positively associated with mortality (all-cause: <i>p</i>-trend = 0.007, cancer: <i>p</i>-trend = 0.020). RCS showed increased risks of all-cause and cancer mortality when total protein-energy ratio exceeded 14.8%. The risk of all-cause and cancer mortality showed a downward trend when isocaloric plant-protein-energy ratios replaced animal-protein-energy ratios. Rats fed high-animal-protein diets showed higher risk for tumorigenesis. HP diet may alter the transcriptome profiles of rat liver, with upregulated ACSM5, AMACR and TM7SF2, and downregulated HAO2. <i>Conclusions</i>: Keeping the total protein-energy ratio below 14.8% may be beneficial to reducing all-cause and cancer mortality. Substitution of animal protein with plant protein (by 2.5%, 5%, and 10% of energy) was associated with a decreasing trend in all-cause and cancer mortality. Animal experiments confirmed that the risk of tumorigenesis was associated with a high animal-protein-energy ratio, highlighting the effects of different sources of protein on health and the mechanisms of cancer development.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between ultra-processed foods and adiposity: findings from a Tehran lipid and glucose study.","authors":"Mahdieh Golzarand, Nazanin Moslehi, Mohadeseh Aghasi, Parvin Mirmiran, Fereidoun Azizi","doi":"10.1039/d4fo06072e","DOIUrl":"https://doi.org/10.1039/d4fo06072e","url":null,"abstract":"<p><p>A few cohort studies have assessed the relationship between ultra-processed foods (UPFs) and obesity; however, there is a knowledge gap about adiposity. We designed the present cohort study to investigate the relationship between UPF consumption and adult adiposity. The adiposity indices considered in the present study included: waist circumference (WC), WC-to-height ratio (WHtR), Clínica Universidad de Navarra-Body Adiposity Estimator (CUN-BAE) index, body roundness index (BRI), a body shape index (ABSI), and visceral adiposity index (VAI). This cohort study included 2734 adults from the Tehran Lipid and Glucose Study (TLGS). Five measurements were available at the baseline (2006-2008) and every three years until the end of follow-up (2018-2023). We applied the NOVA food classification to estimate our population's consumption of UPFs in servings per day. We used mixed linear regression to assess the association between the tertiles of UPFs as servings per day and changes in adiposity indices. The dose-response relationship between UPFs (serving per day) and outcomes was determined using a restricted cubic spline model. The mean age of participants was 39.8 years, and 54.1% were women. The median follow-up time was 11.9 years. The results of linear mixed models demonstrated a positive relationship between consumption of UPFs and weight (<i>β</i> = 0.17, <i>P</i> < 0.0001), body mass index (BMI) (<i>β</i> = 0.05, <i>P</i> = 0.002), CUN-BAE index (<i>β</i> = 0.08, <i>P</i> < 0.0001), WC (<i>β</i> = 0.16, <i>P</i> = 0.001), BRI (<i>β</i> = 0.02, <i>P</i> = 0.02), WHtR (<i>β</i> = 0.001, <i>P</i> = 0.005), and ABSI (<i>β</i> = 0.00006, <i>P</i> = 0.003) in the full model. There was no association between UPFs and the VAI (<i>β</i> = 0.01, <i>P</i> = 0.55). In the dose-response analysis, the association increased significantly after five servings per day. When we considered changes in each outcome per year, we found that the annual increases in weight by 0.12 kg, BMI by 0.04 kg m^-2, WC by 0.12 cm, CUN-BAE index by 0.06 unit, BRI by 0.02 unit, and WHtR by 0.001 unit were greater in the third tertile of UPFs compared with the first tertile. Our results support the positive association between UPFs and adiposity indices.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into the underlying mechanism of functional <i>Pediococcus acidilactici</i> SWU-HX39 in alleviating hyperuricemia.","authors":"Juxin He, Xirui Zhang, Huijing Chen, Jianquan Kan, Sameh Awad, Amel A Ibrahim, Zsolt Zalán, Muying Du","doi":"10.1039/d5fo01404b","DOIUrl":"https://doi.org/10.1039/d5fo01404b","url":null,"abstract":"<p><p>Hyperuricemia (HUA) is a major challenge in the field of public health, and long-term use of traditional drugs can easily lead to addiction and some side effects such as the hypersensitivity syndrome and liver and kidney damage. While several studies have proved that some probiotics are able to reduce serum uric acid (UA) levels, in this study, we found that <i>Pediococcus acidilactici</i> SWU-HX39 (HX39) has superior anti-HUA ability <i>in vitro</i>, and the therapeutic potential of HX39 in a HUA model of a high purine diet was also evaluated <i>in vivo</i>, focusing on its regulatory effects on liver xanthine oxidase (XOD) activity and serum proinflammatory cytokine and lipopolysaccharide (LPS) content. The results of the HUA mouse model showed a significant reduction of 49.8% in serum UA and 46.76% XOD activity by HX39 compared with the control group. Moreover, HX39 regulated the composition of the intestinal flora in mice, with significant decreases of the <i>Bacteroides</i> abundances and <i>Parabacteroides</i>, while <i>Eubacterium_brachy_group</i> and norank_f_<i>Ruminococcaceae</i> abundances increased. Further analysis showed that it helped alleviate inflammation and improve kidney metabolism. Significantly reduced kidney damage was observed in mice with HUA. These results may explain the potential mechanism of functional probiotics in alleviating HUA symptoms. Also, probiotic HX39 has promising applications in the treatment of HUA complications.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How <i>in vitro</i> gastrointestinal digestion impacts the phenolic profile and bioactivities of North American sea buckthorn fruit and seeds.","authors":"Renan Danielski, Fereidoon Shahidi","doi":"10.1039/d5fo01306b","DOIUrl":"https://doi.org/10.1039/d5fo01306b","url":null,"abstract":"<p><p>North American sea buckthorn (<i>Hippophae rhamnoides</i> L.) shows great commercial potential as a bioactive-rich fruit. The pomace and seeds of sea buckthorn fruit are rich sources of a variety of polyphenols with promising antioxidant and biological potential. However, the modification of these phenolics through the gastrointestinal tract is unknown and can significantly affect their bioactivities. Therefore, the pomace and seeds of Newfoundland cultivated sea buckthorn were subjected to <i>in vitro</i> simulated gastrointestinal (GI) digestion and their phenolic composition was monitored across oral, gastric, small and large intestine digestion. Moderate phenolic bioaccessibility was recorded for sea buckthorn pomace and seeds, with phenolic release rates of 35.34 and 44.86% after small intestinal digestion. This fraction contained a variety of proanthocyanidins, while phenolic acids and their derivatives were generally released at the earlier oral and gastric digestion. The phenolic profile of sea buckthorn digestion products was considerably different from undigested samples, reflecting on improved rate of inhibition of metabolic enzymes linked to type 2 diabetes and obesity in some cases, as well as oxidative protection to supercoiled DNA against the action of hydroxyl radicals. However, the capacity of samples to protect LDL-cholesterol from oxidative damage was decreased after digestion. These results represent an estimate of physiological effects, pending validation through <i>in vivo</i> human studies. Further research should concentrate on investigating the absorption and transport of polyphenols involved across the human gut by using relevant physiological models.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory effects of <i>Camellia</i> seed oil on zebrafish and RAW264.7 cells studied using an <i>in vitro</i> bioassay.","authors":"Xufeng Jiang, Weiguo Wang, Yang Yu, Wenxuan Cheng, Xiaonan Cheng, Zhouxin Li, Yang Zhang","doi":"10.1039/d5fo01679g","DOIUrl":"https://doi.org/10.1039/d5fo01679g","url":null,"abstract":"<p><p><i>Camellia</i> seed oil (CSO), extracted from <i>Camellia</i> seeds, contains numerous beneficial bioactive components. While the majority of produced CSO is utilized in food applications, its use in life sciences and health fields remains relatively limited. To expand its applications, this study investigated the potential anti-inflammatory activity of CSO and its underlying molecular mechanism through zebrafish and cellular models. The results demonstrated that CSO promotes neutrophil migration in zebrafish, inhibits oxidative damage occurrence, and enhances repair of damaged caudal fins. Furthermore, in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, CSO suppressed reactive oxygen species (ROS) elevation and prevented the reduction in phagocytic capacity. Additionally, analysis revealed that five-year stored <i>Camellia</i> seed oil (FYSCSO) exhibited superior anti-inflammatory activity compared to newly produced oil, which may be attributed to increased linoleic acid content and decreased oleic acid content. Mechanistic studies have shown that CSO possesses significant anti-inflammatory properties mediated through activation of the NF-κB signaling pathway. This study advances understanding of CSO's biological functions and molecular mechanisms, providing foundational insights for its development in life and health applications.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}