Food & Function最新文献_第10页

A novel method for exploration and prediction of the bioactive target of rice bran-derived peptide (KF-8) by integrating computational methods and experiments† 通过计算方法与实验相结合探索和预测米糠肽（KF-8）生物活性靶点的新方法。

IF 5.1 1区农林科学

Food & Function Pub Date : 2024-11-06 DOI: 10.1039/D4FO02493A

Rui Liang, Fangliang Song, Ying Liang, Yanpeng Fang, Jianqiang Wang, Yajuan Chen, Zhongxu Chen, Xiaorong Tan and Jie Dong

{"title":"A novel method for exploration and prediction of the bioactive target of rice bran-derived peptide (KF-8) by integrating computational methods and experiments†","authors":"Rui Liang, Fangliang Song, Ying Liang, Yanpeng Fang, Jianqiang Wang, Yajuan Chen, Zhongxu Chen, Xiaorong Tan and Jie Dong","doi":"10.1039/D4FO02493A","DOIUrl":"10.1039/D4FO02493A","url":null,"abstract":"The investigation into the bioactive peptide's activity and target action poses a significant challenge in the field of food. An active peptide prepared from rice bran, KF-8, was confirmed to possess antioxidant activity in our previous study, but the specific target was unclear. This study used eight target prediction tools based on artificial intelligence and chemoinformatics to preliminarily screen potential antioxidant targets by integrating different computational methods. Then five different types of docking software were comparatively analyzed to further clarify their interaction sites and possible modes of action. The results showed that SIRT1 and CXCR4 are potential antioxidant targets of KF-8. Different docking software suggested that KF-8 interacts with SIRT1 and CXCR4 as major residues. Meanwhile, the results of Immunofluorescence co-localization experiments showed that the co-localization coefficients of KF-8 with SIRT1 and CXCR4 reached 0.5879 and 0.5684. This study provides new alternative means for the discovery of active peptide targets.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 24","pages":" 11875-11887"},"PeriodicalIF":5.1,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation and quantification of phenolic, anthocyanidin and terpene species in plant foods and plant food-based complementary products with antioxidant and neuroprotective properties† 具有抗氧化和神经保护特性的植物食品和以植物食品为基础的补充产品中酚类、花青素和萜类物质的特性和定量分析。

IF 5.1 1区农林科学

Food & Function Pub Date : 2024-11-05 DOI: 10.1039/D4FO02942A

Naomi May, Jiahua Shi, Helen Clunas, Julianna Lys de Sousa Alves Neri, Celine Kelso, Jody Morgan, Yinghua Yu, Karen Charlton and Katrina Weston-Green

{"title":"Characterisation and quantification of phenolic, anthocyanidin and terpene species in plant foods and plant food-based complementary products with antioxidant and neuroprotective properties†","authors":"Naomi May, Jiahua Shi, Helen Clunas, Julianna Lys de Sousa Alves Neri, Celine Kelso, Jody Morgan, Yinghua Yu, Karen Charlton and Katrina Weston-Green","doi":"10.1039/D4FO02942A","DOIUrl":"10.1039/D4FO02942A","url":null,"abstract":"Phytochemicals, including phenolic compounds and terpenes, are of interest for the treatment and prevention of conditions with oxidative stress, inflammatory and neurodegenerative pathologies. Certain plant foods have shown beneficial effects for the brain; however, the specific phenolic and terpene species in these foods are unclear. The present study aimed to characterise and quantify the phenolic, anthocyanidin and terpene species in six plant foods (Queen Garnet plum (QGP, Prunus salicina); black pepper (BPF, Piper nigrum); clove (CF, Syzygium aromaticum); elderberry (EF, Sambucus nigra); lemon balm (LBF, Melissa officinalis); and sage (SF, Salvia officinalis)) and six plant food-based complementary products (clove (CC), elderberry (EC), lemon balm (LBC), and sage (SC), plus two blends (Astragalus membranaceus and lemon balm-rich, WC and R8)). The relationships between the concentration of phytochemical species in these samples and their antioxidant capacities (i.e. oxygen and nitrogen free radical scavenging, Cu2+ and Fe2+ chelating capacities, and the ability to prevent H2O2-induced oxidative stress in neuroblast-like SH-SY5Y cells, in vitro) were also examined. WC had the highest concentration of phenolics, followed by QGP, EF, CC and CF. BPF had the highest total terpene concentration followed by CC, CF and SF. Correlations between certain compounds and antioxidant capacity were demonstrated. The results provide insight into the potential functional capabilities of species of phenolics and terpenes. Understanding the phytochemical profile of plant foods and their correlations may be important in understanding their potential therapeutic benefits for brain health.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 23","pages":" 11537-11563"},"PeriodicalIF":5.1,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and characterization of white shrimp hydrolysate–xylooligosaccharide Maillard products and their in vivo promotive effects of zinc absorption in mice 白虾水解物-低聚木糖马氏体产物的制备和特性及其对小鼠体内锌吸收的促进作用

IF 5.1 1区农林科学

Food & Function Pub Date : 2024-11-05 DOI: 10.1039/D4FO03709J

Shijie Dou, Xuening Yu, Yuewen Xu, Xiaoyang Liu, Fawen Yin, Deyang Li and Dayong Zhou

{"title":"Preparation and characterization of white shrimp hydrolysate–xylooligosaccharide Maillard products and their in vivo promotive effects of zinc absorption in mice","authors":"Shijie Dou, Xuening Yu, Yuewen Xu, Xiaoyang Liu, Fawen Yin, Deyang Li and Dayong Zhou","doi":"10.1039/D4FO03709J","DOIUrl":"10.1039/D4FO03709J","url":null,"abstract":"The Maillard reaction products, as a kind of glycosylation-based reaction, possess the metal-chelating ability. In this study, the white shrimp hydrolysate (WH) and xylooligosaccharides (XOS) were used to prepare the Maillard reaction product–zinc complex (WH-XOS-MR-Zn) with zinc ions. The Maillard reaction conditions, such as pH level, temperature, reaction time, and the ratio of XOS to WH, were selected by testing the products’ zinc-chelating capacity, while the optimized conditions (zinc-chelating capacity = 64.8%, pH = 7, 110 °C, 180 min, XOS : WH = 2) were finally determined. The interactions between WH-XOS-MR and zinc were confirmed and characterized by various kinds of techniques and triggered new peaks of fluorescent signals. The addition of zinc in WH-XOS-MR induced the proportion changes of secondary structures, including the decrease of β-sheets (8.16%) and the increase of β-turns (5.9%) and random coils (2.23%). The addition of zinc changed the morphological surface appearance of WH-XOS-MR and the crystal signal was completely covered in the WH-XOS-MR-Zn complex involved in the chelation with carbonyl and amino groups. The high-dose and medium-dose of the WH-XOS-MR-Zn complex showed higher promotive effects on zinc absorption (11.89 and 11.05 umol L−1, respectively) and medium-dose recovered values of AKP (liver and serum: 7.15 and 12.53 U mL−1), SOD (liver, kidney and serum: 59.84, 7.86 and 13.61 U mL−1) and GSH-Px (203.22 U per mgprot), damage to testicular tissues, damage to the intact neuron cells in the hippocampus region (CA1: 54 to 61, CA3: 67 to 136, DG: 219–353), and intestinal inflammation compared with the zinc-deficient mice. These findings showed therapeutic benefits of Maillard products on intestinal health and cellular structures.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 23","pages":" 11726-11739"},"PeriodicalIF":5.1,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between vitamin D metabolic pathway-related gene polymorphisms and cardiovascular disease 维生素 D 代谢途径相关基因多态性与心血管疾病之间的相关性。

IF 5.1 1区农林科学

Food & Function Pub Date : 2024-11-04 DOI: 10.1039/D4FO03234A

Jiao Pang, Chunshuo Yang, Jiaqi Liu, Zhilin Wang, Xueshu Tao and Zhipeng Cao

{"title":"Correlation between vitamin D metabolic pathway-related gene polymorphisms and cardiovascular disease","authors":"Jiao Pang, Chunshuo Yang, Jiaqi Liu, Zhilin Wang, Xueshu Tao and Zhipeng Cao","doi":"10.1039/D4FO03234A","DOIUrl":"10.1039/D4FO03234A","url":null,"abstract":"Vitamin D plays important roles in various physiological processes such as cardiovascular health, calcium balance regulation, bone health, immune system support, neurological function regulation, muscle function maintenance, and anti-inflammatory effects. Therefore, maintaining its adequate levels is essential for overall health. Genetic polymorphisms in vitamin D metabolic pathways have become a key factor affecting the susceptibility and progression of cardiovascular disease (CVD). This article reviews the relationship between gene polymorphisms in vitamin D metabolic pathways and vitamin D levels or CVD. It is emphasized that the polymorphisms of key genes such as GC, VDR, CYP2R1, CYP24A1 and CYP27B1 are related to the pathogenesis of CVD. These polymorphisms can regulate serum levels of vitamin D, thereby affecting the susceptibility, comorbidities and clinical manifestations of CVD. Despite the progress made, there are still inconsistencies and gaps in the literature. Thus, it is necessary to conduct large-scale, multicenter studies to verify these findings and deepen our understanding of the intricate interactions between gene polymorphisms in vitamin D metabolic pathways and CVD.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 23","pages":" 11342-11364"},"PeriodicalIF":5.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic treatment to decrease the allergenicity of Pru p 3 from peach† 酶处理降低桃子中 Pru p 3 的过敏性。

IF 5.1 1区农林科学

Food & Function Pub Date : 2024-11-04 DOI: 10.1039/D4FO03052D

Ana P. Tobajas, Ana Agulló-García, José L. Cubero, Carlos Colás, Alba Civera, Clara Esteban, Lourdes Sánchez and María D. Pérez

{"title":"Enzymatic treatment to decrease the allergenicity of Pru p 3 from peach†","authors":"Ana P. Tobajas, Ana Agulló-García, José L. Cubero, Carlos Colás, Alba Civera, Clara Esteban, Lourdes Sánchez and María D. Pérez","doi":"10.1039/D4FO03052D","DOIUrl":"10.1039/D4FO03052D","url":null,"abstract":"Pru p 3, a member of the lipid transfer protein family, is considered a major allergen from peach as it often induces serious allergic reactions in peach-allergic individuals. The high resistance of Pru p 3 to processing treatments and to digestion or enzymatic hydrolysis is probably the cause of the severity of this fruit allergy. The aim of this study was to determine the effect of treatment with a large number of proteases from different origins (vegetal, animal and microbial) on the degradation and allergenicity of Pru p 3. To perform this study, Pru p 3 was previously isolated using cation exchange chromatography and ultrafiltration, and the purified protein was incubated with proteases under different conditions. The results showed that only two of the fifteen proteases assayed were able to efficiently degrade the protein at acidic pH, as determined by SDS-PAGE. These two commercial acid proteases, derived from Aspergillus niger, decreased by more than 95% the immunoreactivity of Pru p 3 by ELISA using specific rabbit IgG, giving peptides lower than 3.2 kDa as determined by MALDI-TOF mass spectrometry. The hydrolysates obtained showed a greater than 70% decrease in reactivity of IgE compared to untreated Pru p 3 using three pools of sera from peach allergic individuals. Furthermore, when hydrolysates were tested by the prick test, in more than 90% of peach-allergic patients the average size of the wheal significantly decreased by between 72% and 85%. The results suggest that the acid protease from Aspergillus niger could be used to obtain novel hypoallergenic products more tolerable for peach-sensitive individuals.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 24","pages":" 12007-12015"},"PeriodicalIF":5.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/fo/d4fo03052d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review on mechanisms of hypoglycemic effects of compounds from highland barley and potential applications† 高原大麦化合物降血糖作用机制及潜在应用综述。

IF 5.1 1区农林科学

Food & Function Pub Date : 2024-11-04 DOI: 10.1039/D4FO00940A

Yanan Liu, Yuanqiang Jia, Yingying Wu, Huijuan Zhang, Feiyue Ren and Sumei Zhou

引用次数: 0

Interaction of oat bran and exercise training improved exercise adaptability via alleviating oxidative stress and promoting energy homeostasis† 燕麦麸皮与运动训练的相互作用通过减轻氧化应激和促进能量平衡提高了运动适应性。

IF 5.1 1区农林科学

Food & Function Pub Date : 2024-11-04 DOI: 10.1039/D4FO03374D

Rui Dong, Hamad Rafique, Qianwen Niu, Xin Zeng, Maria Cristina Messia, Li Yuan, Lin Shi, Liang Zou, Lu Li and Xinzhong Hu

{"title":"Interaction of oat bran and exercise training improved exercise adaptability via alleviating oxidative stress and promoting energy homeostasis†","authors":"Rui Dong, Hamad Rafique, Qianwen Niu, Xin Zeng, Maria Cristina Messia, Li Yuan, Lin Shi, Liang Zou, Lu Li and Xinzhong Hu","doi":"10.1039/D4FO03374D","DOIUrl":"10.1039/D4FO03374D","url":null,"abstract":"Skeletal muscle performance is influenced by both diet and the mode of exercise, with diet playing a crucial role in individuals’ adaptation to exercise training. Our study investigated the interaction of oat bran (OB) diet and moderate intensity exercise training (MIET) on skeletal muscle function and athletic performance. Studies have reinforced the positive association of high-fat diet (HFD) with chronic systemic inflammation and corresponding peripheral skeletal muscle dysfunction during exercise training. OB could alleviate the inflammation, oxidative stress, and energy homeostasis disorder associated with HFD. We observed improvement in mice limb grip strength and endurance treadmill running distance with OB intake, accompanied by regulation of muscle function-related gene expression. OB intensified exercise training-induced carbohydrate and lipid metabolism, as indicated by changes in lactate, fumarate, malate, pyruvate, succinate, and citrate levels. Additionally, specific probiotic genera producing short-chain fatty acids (SCFAs) were promoted, while inflammation-related circulating metabolites were significantly decreased with oat bran intake. Our findings suggest interactions between OB and MIET improved HFD-induced skeletal muscle dysfunction on both the phenotype and the related mechanisms. This study is an extension of our previous study on the anti-fatigue effect of oat bran, providing a novel prospective by integrating exercise adaptation, gut microbiota, molecular metabolism and skeletal muscle in situ analysis.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 23","pages":" 11508-11524"},"PeriodicalIF":5.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between olive oil consumption and all-cause, cardiovascular and cancer mortality in adult subjects: a systematic review and meta-analysis† 成年受试者食用橄榄油与全因、心血管和癌症死亡率之间的关系：系统回顾和荟萃分析。

IF 5.1 1区农林科学

Food & Function Pub Date : 2024-11-01 DOI: 10.1039/D4FO04161E

Andrea del Saz-Lara, Alicia Saz-Lara, Iván Cavero-Redondo, Carlos Pascual-Morena, Carmen Mazarío-Gárgoles, Francesco Visioli, María-Carmen López de las Hazas and Alberto Dávalos

{"title":"Association between olive oil consumption and all-cause, cardiovascular and cancer mortality in adult subjects: a systematic review and meta-analysis†","authors":"Andrea del Saz-Lara, Alicia Saz-Lara, Iván Cavero-Redondo, Carlos Pascual-Morena, Carmen Mazarío-Gárgoles, Francesco Visioli, María-Carmen López de las Hazas and Alberto Dávalos","doi":"10.1039/D4FO04161E","DOIUrl":"10.1039/D4FO04161E","url":null,"abstract":" Background: The Mediterranean diet is characterized by the preferential use of olive oil (OO) and has been associated with health benefits possibly mediated by its bioactive compounds. Objectives: We aimed to analyze the association between OO consumption and changes in all-cause mortality, cardiovascular mortality and cancer mortality in the adult population. Methods: A systematic review was conducted using the Pubmed, Scopus, Web of Science and Cochrane Library databases up to October 2024. DerSimonian and Laird's random effects method was used to calculate pooled estimates of hazard ratios (HRs) and their 95% confidence intervals (95% CIs) to determine the association between OO use and changes in all-cause mortality, cardiovascular mortality, and cancer mortality. Results: Fourteen studies were included in the systematic review and seven in the meta-analysis. Our results showed an association between OO consumption and reduction in all-cause mortality (HR: 0.85; 95% CI: 0.80–0.91), cardiovascular mortality (HR: 0.84; 95% CI: 0.76–0.93) and cancer mortality (HR: 0.89; 95% CI: 0.86–0.93). Conclusions: Consumption of OO particularly reduces cardiovascular mortality (16%), followed by all-cause mortality (15%) and cancer mortality (11%) in the adult population. However, further studies are needed to clarify the underlying mechanisms.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 23","pages":" 11640-11649"},"PeriodicalIF":5.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-obesity and other health benefits of bioprocessed black rice bran in combination with green tea extract in 3T3-L1 preadipocyte cells and in mice on a high-fat diet 生物加工黑米糠与绿茶提取物在 3T3-L1 前脂肪细胞和高脂饮食小鼠中的抗肥胖及其他健康益处。

IF 5.1 1区农林科学

Food & Function Pub Date : 2024-11-01 DOI: 10.1039/D4FO03210A

Ki Sun Kwon, Eun Seok Lee, Kyung Hee Lee, Woon Sang Hwang, Wha Young Lee, Jae Jung Kim, Jeanman Kim, Sang Jong Lee, Sung Phil Kim and Mendel Friedman

{"title":"Anti-obesity and other health benefits of bioprocessed black rice bran in combination with green tea extract in 3T3-L1 preadipocyte cells and in mice on a high-fat diet","authors":"Ki Sun Kwon, Eun Seok Lee, Kyung Hee Lee, Woon Sang Hwang, Wha Young Lee, Jae Jung Kim, Jeanman Kim, Sang Jong Lee, Sung Phil Kim and Mendel Friedman","doi":"10.1039/D4FO03210A","DOIUrl":"10.1039/D4FO03210A","url":null,"abstract":"Black rice bran, a waste product from the commercial milling of black rice that removes the bran and germ leaving the starchy endosperm, contains bioactive anthocyanin, phenolic, and phytosteroid compounds that may have health benefits. This study determined the effect of a polysaccharide-rich bioprocessed (fermented) black rice bran and a green tea extract individually and in combination on weight loss in orally fed mice on a high-fat diet and on concurrent changes in blood glucose and insulin as well as in cholesterol, triglyceride, and high-density and low-density lipoproteins (HDL and LDL). At the end of the eight-week feeding study, the combination diet resulted in a 67% lower weight gain than mice on a high-fat diet alone, a greater effect than that of bioprocessed black rice bran or green tea extract individually. The weight loss caused by the combination diet seems to be the result of decreased dietary efficiency. The observed trends in the glucose and insulin data suggest that the combined diet also has anti-diabetic properties, and the corresponding trends in the levels of the serum lipoproteins suggest that the combined diet might also protect against heart disease. Effects on the content, structure, and function of white adipose and liver tissues and on obesity-related biomarkers support the trends in the weight loss data. Based on the observed beneficial effects in 3T3-L1 pre-adipocyte cells and mice, we suggest the need to investigate if the new multifunctional combination food product can also protect against obesity and chronic diseases in humans. Mechanistic aspects that govern the anti-obesity effects and suggestions for future research are discussed.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 24","pages":" 12083-12100"},"PeriodicalIF":5.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo mechanism of the interaction between trimethylamine lyase expression and glycolytic pathways† 三甲胺裂解酶的表达与糖酵解途径相互作用的体内机制。

IF 5.1 1区农林科学

Food & Function Pub Date : 2024-10-31 DOI: 10.1039/D4FO03809F

Qian Li, Di Wu, Yu Song, Lu Zhang, Ting Wang, Xiaoxu Chen and Min Zhang

{"title":"In vivo mechanism of the interaction between trimethylamine lyase expression and glycolytic pathways†","authors":"Qian Li, Di Wu, Yu Song, Lu Zhang, Ting Wang, Xiaoxu Chen and Min Zhang","doi":"10.1039/D4FO03809F","DOIUrl":"10.1039/D4FO03809F","url":null,"abstract":"Recent studies confirmed that host–gut microbiota interactions modulate disease-linked metabolite TMA production via TMA lyase. However, microbial enzyme production mechanisms remain unclear. In the present study, we investigated the impact of dietary and intervention factors on gut microbiota, microbial gene expression, and the interplay between TMA lyase and glycolytic pathways in mice. Using 16S rRNA gene sequencing, metagenomics, and metabolomics, the gut microbiota composition and microbial functional gene expression profiles related to TMA lyase and glycolytic enzymes were determined. The results revealed that distinct diets and intervention factors altered gut microbiota, gene expression, and metabolites linked to glycine metabolism and glycolysis. Notably, an arabinoxylan-rich diet suppressed genes linked to choline, glycine, glycolysis, and TMA lyase, favoring glycine utilization via pyruvate pathways. Glycolytic inhibitors amplified these effects, mainly inhibiting pyruvate kinase. Our findings underscored the crosstalk between TMA lyase and glycolytic pathways, regulating glycine levels, and suggested avenues for targeted interventions and personalized diets to curb choline TMA lyase production.","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 1","pages":" 87-101"},"PeriodicalIF":5.1,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0