Irene Ferrarese, Maria Giovanna Lupo, Ilaria Rossi, Giorgia Marodin, Haixia Chen, Stefania Sut, Nicola Ferri and Stefano Dall'Acqua
{"title":"番石榴提取物、精油及分离化合物对人肝癌Huh7细胞LDL受体和PCSK9表达的影响","authors":"Irene Ferrarese, Maria Giovanna Lupo, Ilaria Rossi, Giorgia Marodin, Haixia Chen, Stefania Sut, Nicola Ferri and Stefano Dall'Acqua","doi":"10.1039/D5FO01511A","DOIUrl":null,"url":null,"abstract":"<p >Peel (MPLE), pulp extract (MPPE) and essential oil (MPO) of <em>Citrus</em> × <em>tangelo</em> were studied for their chemical composition and for their ability to act on the expression of the low-density lipoprotein (LDL) receptor and proprotein convertase subtilisin/kexin 9 (PCSK9), two key players in cholesterol metabolism, in the human hepatocarcinoma cell line Huh7. MPPE demonstrated the ability to induce the LDL receptor and to reduce PCSK9. Chemical characterization of the extracts was conducted <em>via</em> LC-DAD-MS for MPLE and MPPE and <em>via</em> GC-MS for MPO. The main compounds isolated from MPLE and MPPE were ferulic acid, narirutin, 3′-methoxy-narirutin, nobiletin, 3-methoxynobiletin and tangeretin. MPPE significantly induced the LDL receptor (+1.43 ± 0.49-fold <em>vs.</em> basal) and suppressed PCSK9 levels (−64% ± 24% <em>vs.</em> basal). Among the different isolated compounds, ferulic acid showed the most interesting modulation of both the LDL receptor (+1.26 ± 0.14-fold <em>vs.</em> basal) and PCSK9 (−59% ± 14% <em>vs.</em> basal), showing potential cholesterol-lowering properties.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 19","pages":" 7823-7834"},"PeriodicalIF":5.4000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d5fo01511a?page=search","citationCount":"0","resultStr":"{\"title\":\"Effects of ‘Mapo’ tangelo (Citrus × tangelo) extracts, essential oil, and isolated compounds on LDL receptor and PCSK9 expression in the human hepatocarcinoma cell line Huh7\",\"authors\":\"Irene Ferrarese, Maria Giovanna Lupo, Ilaria Rossi, Giorgia Marodin, Haixia Chen, Stefania Sut, Nicola Ferri and Stefano Dall'Acqua\",\"doi\":\"10.1039/D5FO01511A\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Peel (MPLE), pulp extract (MPPE) and essential oil (MPO) of <em>Citrus</em> × <em>tangelo</em> were studied for their chemical composition and for their ability to act on the expression of the low-density lipoprotein (LDL) receptor and proprotein convertase subtilisin/kexin 9 (PCSK9), two key players in cholesterol metabolism, in the human hepatocarcinoma cell line Huh7. MPPE demonstrated the ability to induce the LDL receptor and to reduce PCSK9. Chemical characterization of the extracts was conducted <em>via</em> LC-DAD-MS for MPLE and MPPE and <em>via</em> GC-MS for MPO. The main compounds isolated from MPLE and MPPE were ferulic acid, narirutin, 3′-methoxy-narirutin, nobiletin, 3-methoxynobiletin and tangeretin. MPPE significantly induced the LDL receptor (+1.43 ± 0.49-fold <em>vs.</em> basal) and suppressed PCSK9 levels (−64% ± 24% <em>vs.</em> basal). Among the different isolated compounds, ferulic acid showed the most interesting modulation of both the LDL receptor (+1.26 ± 0.14-fold <em>vs.</em> basal) and PCSK9 (−59% ± 14% <em>vs.</em> basal), showing potential cholesterol-lowering properties.</p>\",\"PeriodicalId\":77,\"journal\":{\"name\":\"Food & Function\",\"volume\":\" 19\",\"pages\":\" 7823-7834\"},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2025-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d5fo01511a?page=search\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food & Function\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/fo/d5fo01511a\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food & Function","FirstCategoryId":"97","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/fo/d5fo01511a","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Effects of ‘Mapo’ tangelo (Citrus × tangelo) extracts, essential oil, and isolated compounds on LDL receptor and PCSK9 expression in the human hepatocarcinoma cell line Huh7
Peel (MPLE), pulp extract (MPPE) and essential oil (MPO) of Citrus × tangelo were studied for their chemical composition and for their ability to act on the expression of the low-density lipoprotein (LDL) receptor and proprotein convertase subtilisin/kexin 9 (PCSK9), two key players in cholesterol metabolism, in the human hepatocarcinoma cell line Huh7. MPPE demonstrated the ability to induce the LDL receptor and to reduce PCSK9. Chemical characterization of the extracts was conducted via LC-DAD-MS for MPLE and MPPE and via GC-MS for MPO. The main compounds isolated from MPLE and MPPE were ferulic acid, narirutin, 3′-methoxy-narirutin, nobiletin, 3-methoxynobiletin and tangeretin. MPPE significantly induced the LDL receptor (+1.43 ± 0.49-fold vs. basal) and suppressed PCSK9 levels (−64% ± 24% vs. basal). Among the different isolated compounds, ferulic acid showed the most interesting modulation of both the LDL receptor (+1.26 ± 0.14-fold vs. basal) and PCSK9 (−59% ± 14% vs. basal), showing potential cholesterol-lowering properties.
期刊介绍：
Food & Function provides a unique venue for physicists, chemists, biochemists, nutritionists and other food scientists to publish work at the interface of the chemistry, physics and biology of food. The journal focuses on food and the functions of food in relation to health.
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