IF 6.7 1区化学

Talanta Pub Date : 2027-03-01 Epub Date: 2026-10-17 DOI: 10.1016/j.talanta.2026.130799

Hyeon-Geun Jang, Ramalingam Manikandan, Sung Min Moon, Uma Aruljothi Mugunthan, JeongIn Lee, Geon Woo Kang, Jinyong Park, Jang-Hee Yoon, Jaewon Lee, Seung-Cheol Chang

{"title":"Sulfur-doped graphitic carbon nitride/cerium tungstate nanocomposites for ultrasensitive dopamine sensing in mouse striatal tissue.","authors":"Hyeon-Geun Jang, Ramalingam Manikandan, Sung Min Moon, Uma Aruljothi Mugunthan, JeongIn Lee, Geon Woo Kang, Jinyong Park, Jang-Hee Yoon, Jaewon Lee, Seung-Cheol Chang","doi":"10.1016/j.talanta.2026.130799","DOIUrl":"10.1016/j.talanta.2026.130799","url":null,"abstract":"Following the COVID-19 pandemic, the demand for rapid diagnostic tools for the highly specific and sensitive detection of disease-related biomarkers has increased significantly. Hybrid nanocomposite-modified disposable screen-printed electrodes have shown remarkable potential for the electrochemical biosensing of various biomarkers. This study reports the fabrication of a hybrid nanocomposite comprising sulfur-doped graphitic carbon nitride (S-g-C3N4) nanosheets integrated with two-dimensional cerium tungstate (CeW) for highly sensitive electrochemical dopamine detection. The synthesized S-g-C3N4@CeW hybrid nanocomposite was thoroughly characterized using field-emission scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, and electrochemical measurements. Electroanalytical performance was evaluated using differential pulse voltammetry (DPV) under optimized experimental conditions. The fabricated electrochemical sensor exhibited a wide linear concentration range for dopamine from 0.1 to 500 μM, with a low detection limit of 1.13 nM and high sensitivity of 151.69 μA μM-1 cm-2. Finally, the fabricated sensor was used for the ex-vivo determination of dopamine in Parkinson's disease-induced mouse striatum-tissue samples, achieving good accuracy with recovery rates of 92.0 to 101.4%. This study demonstrates a promising strategy for fabricating hybrid nanocomposite-modified disposable electrochemical sensors for neurotransmitter detection in animal-disease models.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"313 Pt A","pages":"130799"},"PeriodicalIF":6.7,"publicationDate":"2027-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149226809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fe-porphyrin MOF nanozyme-based colorimetric sensor assisted by machine learning for detecting antioxidants in fruit juices. 基于fe -卟啉MOF纳米酶的比色传感器，机器学习辅助检测果汁中的抗氧化剂。

IF 6.7 1区化学

Talanta Pub Date : 2027-03-01 Epub Date: 2026-10-19 DOI: 10.1016/j.talanta.2026.130805

Yijie Kong, Xinyu Feng, Xindan Su, Yanming Bi, Lu Zhang, Sha Liu

{"title":"Fe-porphyrin MOF nanozyme-based colorimetric sensor assisted by machine learning for detecting antioxidants in fruit juices.","authors":"Yijie Kong, Xinyu Feng, Xindan Su, Yanming Bi, Lu Zhang, Sha Liu","doi":"10.1016/j.talanta.2026.130805","DOIUrl":"10.1016/j.talanta.2026.130805","url":null,"abstract":"Antioxidants (AOs) are key bioactive components governing the nutritional quality and oxidative stability of fruit juices. However, their similar reducing properties make it challenging for conventional single-signal colorimetric methods to combine individual quantitative analysis with pattern recognition in complex food matrices. Herein, an Fe-porphyrin metal-organic framework (FeP-MOF) nanozyme was constructed using iron(III) meso-tetra(4-carboxyphenyl)porphyrin chloride (Fe-TCPPCl) as the functional ligand, and further integrated into a platform for high-throughput quantification and machine-learning-assisted pattern recognition. The porous MOF framework enhanced the accessibility and catalytic efficiency of Fe-porphyrin active sites. Based on AOs-mediated inhibition of the FeP-MOF/H2O2/TMB oxidation reaction, a microplate-based colorimetric sensor was developed for quantitative detection of ascorbic acid, glutathione, cysteine, gallic acid, and tannic acid, with limits of detection of 0.52-41.54 μM. Moreover, a multi-pH cross-reactive sensor array converted single absorbance signals into composition-sensitive multidimensional fingerprints. Coupled with linear discriminant analysis and hierarchical cluster analysis, the array achieved cross-validated classification accuracies of 100.0% for five antioxidants and 98.0% for five fruit-juice categories. Results also agreed well with high-performance liquid chromatography measurements. This work integrates nanozyme amplification, quantitative analysis of individual antioxidants, fingerprint sensing, and data-driven classification, providing a versatile strategy for high-throughput AOs analysis and compositional evaluation in complex food systems.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"313 Pt A","pages":"130805"},"PeriodicalIF":6.7,"publicationDate":"2027-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149226812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterojunction-induced interface barrier modulation in ZnO@Zn-HHTP for dopamine detection. 异质连接诱导的界面屏障调节ZnO@Zn-HHTP对多巴胺的检测。

IF 6.7 1区化学

Talanta Pub Date : 2027-03-01 Epub Date: 2026-10-19 DOI: 10.1016/j.talanta.2026.130804

Zhanglin Weng, Zhijie Xie, Zhonghui Chen, Cuiying Lin, Bin Qiu, Chuanhui Huang, Zhenyu Lin

{"title":"Heterojunction-induced interface barrier modulation in ZnO@Zn-HHTP for dopamine detection.","authors":"Zhanglin Weng, Zhijie Xie, Zhonghui Chen, Cuiying Lin, Bin Qiu, Chuanhui Huang, Zhenyu Lin","doi":"10.1016/j.talanta.2026.130804","DOIUrl":"10.1016/j.talanta.2026.130804","url":null,"abstract":"Rational modulation of interfacial charge-transfer processes is critical for advancing the performance of electrochemical sensors, yet precise control of heterojunction-induced potential barriers for selective molecular recognition remains challenging. Herein, we report a ZnO@Zn-2,3,6,7,10,11-hexahydroxytriphenylene (HHTP) core-shell heterostructure that enables highly selective detection of dopamine (DA) via analyte-responsive modulation of an interfacial potential barrier. The heterostructure is synthesized through an in-situ conversion strategy, transforming a ZIF-8 shell on ZnO nanorods into Zn-HHTP while preserving the core-shell architecture. The resulting ZnO/Zn-HHTP interface forms a well-defined n-p heterojunction with a built-in potential barrier that governs charge-transfer kinetics. Positively charged DA induces interfacial charge redistribution at the negatively charged Zn-HHTP surface, lowering the barrier height and facilitating electron transfer, thereby amplifying the electrochemical signal. In contrast, common interferents induce negligible response, as they are unable to modulate the interfacial potential barrier and do not undergo effective electrochemical reaction at the characteristic redox potential of dopamine, thereby enabling selective detection. The sensor displays a wide linear range (50 nM-300 μM) and a low detection limit of 16.7 nM, which demonstrates reliable performance in the detection of target in human urine samples. This work highlights interfacial barrier modulation as an effective strategy for designing high-performance electrochemical sensors based on conductive MOF-semiconductor heterostructures.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"313 Pt A","pages":"130804"},"PeriodicalIF":6.7,"publicationDate":"2027-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic integrated nucleic acid test (MINT) enabled by coupling FEN1-aided RPA with wax-purification and bead-carrier. 将fen1辅助RPA与蜡质纯化和珠状载体耦合，实现微流控集成核酸检测（MINT）。

IF 6.7 1区化学

Talanta Pub Date : 2027-03-01 Epub Date: 2026-10-19 DOI: 10.1016/j.talanta.2026.130801

Zhaoluo Xu, Xingyue Gao, Jinling Huang, Shuyun Pang, Shanshan Wang, Yuanmeng Wang, Liying Feng, Yi Ma, Haiping Wu, Guohua Zhou

{"title":"Microfluidic integrated nucleic acid test (MINT) enabled by coupling FEN1-aided RPA with wax-purification and bead-carrier.","authors":"Zhaoluo Xu, Xingyue Gao, Jinling Huang, Shuyun Pang, Shanshan Wang, Yuanmeng Wang, Liying Feng, Yi Ma, Haiping Wu, Guohua Zhou","doi":"10.1016/j.talanta.2026.130801","DOIUrl":"10.1016/j.talanta.2026.130801","url":null,"abstract":"To guide timely antibiotic treatment, a tool for quick antimicrobial resistance gene detection is required. Here a Microfluidic Integrated Nucleic Acid Test platform (namely, MINT) was proposed for point-of-care molecular diagnostics. The platform integrates fast nucleic acid extraction based on hybrid wax-phase purification, and DNA amplification based on our newly developed FEN1-aided RPA (FARPA) into a microfluidic chip. The wax-phase stays solid at room temperature, while becomes liquid at >50 °C to allow the wash of inhibitors carried from extraction buffer, thus the chip keeps stable during storage and transportation. The movement of extracted DNA bound with magnetic beads were controlled by a magnet. The beaded DNA was quickly purified during the flow through the wax phase, and the amplification started once the beaded DNA reached to the well for FARPA. The signal from target-positive sample could be readily sensed by a conventional smartphone. The turnaround time is about 40 min from the sample to the result. The platform was validated by detecting the blaKPC-2 resistance gene of Klebsiella pneumoniae (K. pneumoniae) from urine samples with the reliable detection concentration of 500 CFU/mL, achieving 100% agreement for the 29 clinical urine samples (19 positive, 10 negative) with conventional qPCR. It is easy to adapt the MINT to any other pathogen only by designing the corresponding primers and probes for FARPA. It is promising to apply MINT for bed-site antimicrobial resistance screening just after operation.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"313 Pt A","pages":"130801"},"PeriodicalIF":6.7,"publicationDate":"2027-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149226865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-destructive biochemical profiling of goldenberry ripening using confocal micro Raman spectroscopy coupled with machine learning. 利用共聚焦微拉曼光谱结合机器学习对金梅成熟过程进行无损生化分析。

IF 6.7 1区化学

Talanta Pub Date : 2027-02-01 Epub Date: 2026-08-05 DOI: 10.1016/j.talanta.2026.130388

Aishwary Awasthi, Aradhana Tripathi, Shristi Sharma, Aparna Tiwari, Chhavi Baran, Sweta Sharma, K N Uttam

{"title":"Non-destructive biochemical profiling of goldenberry ripening using confocal micro Raman spectroscopy coupled with machine learning.","authors":"Aishwary Awasthi, Aradhana Tripathi, Shristi Sharma, Aparna Tiwari, Chhavi Baran, Sweta Sharma, K N Uttam","doi":"10.1016/j.talanta.2026.130388","DOIUrl":"10.1016/j.talanta.2026.130388","url":null,"abstract":"The accurate monitoring of the fruit ripening is crucial for maintaining quality and prevention from post-harvest economic losses. Goldenberry, being rich in vitamins, minerals, and carotenoids, yet its ripening assessment relies on variable and often subjective traditional approaches. To address this, the present study utilises rapid and non-destructive techniques using confocal micro-Raman and UV-Vis spectroscopy to track biochemical changes in both the exocarp and mesocarp of goldenberry fruit across five ripening stages: pre-mature, mature, pre-ripe, ripe, and post-ripe. The analysis of Raman spectra of exocarp shows an increase in carotenoid bands up to ripe stage, followed by a decrease at post-ripe stage, while the mesocarp exhibits a continuous increase in carotenoid intensity through stage post-ripe. The UV-Vis spectral analysis supports these observations, indicating increasing carotenoid content alongside a reduction in chlorophyll as ripening progresses. Among different machine learning (ML) approaches, unsupervised clustering demonstrates strong performance of k-means and agglomerative nesting, with high rand index values (>0.97). In supervised domain, support vector machine, logistic regression, random forest, decision tree, and principal component-linear discriminant analysis achieves the highest values of performance metrices including accuracy, precision, recall, F1 score, ROC/AUC values, and cross validation scores. Moreover, partial least squares regression analysis demonstrates excellent predictive power, with R2 = 0.93 (calibration) and 0.70 (cross-validation) for exocarp and R2 = 0.91 (calibration) and 0.89 (cross-validation) for mesocarp. The combined use of Raman with ML enables a practical and reliable framework for assessing fruit ripening, with promising applications in quality control and post-harvest management.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130388"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148710940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption-regulated electrochemical determination of 17α-ethinylestradiol based on a Cu@Fe₂O₃ composite-modified electrode. Cu@Fe2O3复合修饰电极吸附调控电化学测定17α-炔雌醇。

IF 6.7 1区化学

Talanta Pub Date : 2027-02-01 Epub Date: 2026-08-08 DOI: 10.1016/j.talanta.2026.130407

Meng Li, Xiaowei Yang, Liwei Yang, Huiping Bai, Fengzhi Jiang

{"title":"Adsorption-regulated electrochemical determination of 17α-ethinylestradiol based on a Cu@Fe2O3 composite-modified electrode.","authors":"Meng Li, Xiaowei Yang, Liwei Yang, Huiping Bai, Fengzhi Jiang","doi":"10.1016/j.talanta.2026.130407","DOIUrl":"10.1016/j.talanta.2026.130407","url":null,"abstract":"17α-Ethinylestradiol (EE2) is a phenolic estrogenic endocrine disruptor widely distributed in aquatic environments; therefore, sensitive and reliable analytical methods for its environmental monitoring are required. Herein, an adsorption-regulated electrochemical sensing strategy is proposed for the quantitative determination of EE2 using a Cu@Fe2O3 composite-modified glassy carbon electrode. The synergistic Cu-Fe2O3 interface effectively enhances interfacial affinity toward EE2 molecules and facilitates proton-coupled electron transfer during electrochemical oxidation, resulting in an analytical signal predominantly governed by interfacial adsorption rather than diffusion. Under optimized conditions, the modified electrode exhibits a good linear response to EE2 in the concentration range of 0.10 -20.00 μM with a detection limit of 0.033 μM. Electrochemical mechanism studies indicate that the oxidation of EE2 is an irreversible, adsorption-controlled process. The proposed sensor shows satisfactory selectivity, good repeatability, and long-term stability. Moreover, it was successfully applied to the determination of EE2 in real water samples with acceptable recovery values. This study highlights the crucial role of interfacial adsorption in electrochemical signal amplification and provides a versatile analytical strategy for the sensitive detection of phenolic endocrine-disrupting compounds in complex water matrices.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130407"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148705067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An automated flow-injection thin-layer electrochemical platform for online silicate monitoring in marine environments. 用于海洋环境硅酸盐在线监测的自动流动注入薄层电化学平台。

IF 6.7 1区化学

Talanta Pub Date : 2027-02-01 Epub Date: 2026-08-05 DOI: 10.1016/j.talanta.2026.130406

Hong Wei, Qunqun Liu, Zhiyuan Zhao, Yan Liang, Dawei Pan, Jing Xu

{"title":"An automated flow-injection thin-layer electrochemical platform for online silicate monitoring in marine environments.","authors":"Hong Wei, Qunqun Liu, Zhiyuan Zhao, Yan Liang, Dawei Pan, Jing Xu","doi":"10.1016/j.talanta.2026.130406","DOIUrl":"10.1016/j.talanta.2026.130406","url":null,"abstract":"Silicate is an essential nutrient in marine ecosystems, exhibiting a dynamic concentration due to complex biogeochemical processes. Accurate and high-frequency monitoring of silicate in marine environments is therefore highly desirable. In this study, a fully automated electrochemical system was developed for online determination of silicate in natural seawater and in diatom incubation experiments. This system integrates flow injection analysis with thin-layer electrochemistry (TLE), using square wave voltammetry (SWV) for silicate measurement. The optimized system achieved a low detection limit of 0.4 μM and demonstrated excellent long-term stability, with a relative standard deviation (RSD) of 7.4% over 90 days of operation. The method was validated using coastal seawater collected from 10 sites spanning a range of salinities, showing good agreement with a reference spectrophotometric method. Moreover, continuous monitoring over 7 days in a Phaeodactylum tricornutum culture captured significant changes in the silicate concentration during its growth cycle. Overall, the system provides sensitive, long-term online monitoring of silicate in laboratory-controlled seawater systems and provides a technical basis for future field-deployable applications.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130406"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148696799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A molecularly imprinted electrochemical aptasensor based on graphene and tin dioxide composite with electrodeposited gold nanoparticles for dual recognition of enrofloxacin. 基于石墨烯和二氧化锡复合材料的分子印迹电化学传感器及其对恩诺沙星的双重识别。

IF 6.7 1区化学

Talanta Pub Date : 2027-02-01 Epub Date: 2026-08-03 DOI: 10.1016/j.talanta.2026.130391

Ying Wang, Shanshan Fu, Zhengyuan Dai, Yinuo Liu, Xiaoyu Yang, Li Li, Yaping Ding

{"title":"A molecularly imprinted electrochemical aptasensor based on graphene and tin dioxide composite with electrodeposited gold nanoparticles for dual recognition of enrofloxacin.","authors":"Ying Wang, Shanshan Fu, Zhengyuan Dai, Yinuo Liu, Xiaoyu Yang, Li Li, Yaping Ding","doi":"10.1016/j.talanta.2026.130391","DOIUrl":"10.1016/j.talanta.2026.130391","url":null,"abstract":"In this work, a molecularly imprinted electrochemical aptamer sensor (MIEAS) for the detection of enrofloxacin (ENR) was developed using SnO2-graphene (SnO2-Gr) and gold nanoparticles (AuNPs) as electrode modification materials. The SnO2-Gr composite material has a large specific surface area and can enhance the conductivity of the sensing platform. The aptamer, serving as a biofunctional monomer, was immobilized on the electrode surface via Au-S bonds. Using ENR as the template molecule and nicotinamide (NA) as the chemical functional monomer, a molecularly imprinted polymer (MIP) film was prepared by electropolymerization. Benefiting from the synergistic effect of MIP and aptamer, this MIEAS exhibits a good linear relationship with ENR in the range of 10-14 to 10-5 M, and the detection limit is as low as 1.65 × 10-15 M. Satisfactory recovery was obtained in milk and environmental water samples, indicating the potential for application in food and environmental analysis.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130391"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148696781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-enzymatic colorimetric strategy for malathion detection based on Pt nanoparticles decorated porphyrin Zr metal-organic framework spheres. 基于Pt纳米粒子修饰卟啉Zr金属-有机骨架球的马拉硫磷非酶比色检测策略。

IF 6.7 1区化学

Talanta Pub Date : 2027-02-01 Epub Date: 2026-08-06 DOI: 10.1016/j.talanta.2026.130413

Qing Zhang, Kuizhi Li, Wanzhu Wang, Danhua Ge, Xiaojun Chen

{"title":"Non-enzymatic colorimetric strategy for malathion detection based on Pt nanoparticles decorated porphyrin Zr metal-organic framework spheres.","authors":"Qing Zhang, Kuizhi Li, Wanzhu Wang, Danhua Ge, Xiaojun Chen","doi":"10.1016/j.talanta.2026.130413","DOIUrl":"10.1016/j.talanta.2026.130413","url":null,"abstract":"The presence of malathion (Mal) residues in food and water environment have garnered widespread public concern. Here, we developed PCN-224-supported Pt nanozymes through an in-situ reduction method, where the porous porphyrin framework and Pt sites jointly promote electron transfer and provide more active centers, leading to drastically enhanced oxidase (OXD)-mimicking activity. The obtained PCN-224@Pt can directly oxidize colorless TMB to blue oxTMB without H2O2, while the specific adsorption of Mal on the nanozyme surface will shield active sites and inhibit the catalytic reaction. Accordingly, a colorimetric sensor was then established to achieve sensitive determination of Mal in a linear detection range from 0.01 to 2.5 μg/mL with a low detection limit (LOD) of 6.62 ng/mL. Moreover, this platform displayed high selectivity and stability in food and water samples, with satisfactory recoveries of 90.1 %-107.7 % (RSDs < 9.31 %). This strategy provides a new attempt to construct nanozyme probes for direct detection of Mal, suitable for visual analysis in food and water environments.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130413"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148696802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-assembled DNA nanosphere-powered confined-free self-circulating amplifier for high-fidelity microRNA imaging and clinical liquid biopsy. 用于高保真microRNA成像和临床液体活检的自组装DNA纳米球驱动的无约束自循环放大器。

IF 6.7 1区化学

Talanta Pub Date : 2027-02-01 Epub Date: 2026-08-07 DOI: 10.1016/j.talanta.2026.130416

Jing Jiang, Guohai Lin, Hengxin Yu, Liqun Chen, Huo Xu

{"title":"Self-assembled DNA nanosphere-powered confined-free self-circulating amplifier for high-fidelity microRNA imaging and clinical liquid biopsy.","authors":"Jing Jiang, Guohai Lin, Hengxin Yu, Liqun Chen, Huo Xu","doi":"10.1016/j.talanta.2026.130416","DOIUrl":"10.1016/j.talanta.2026.130416","url":null,"abstract":"The development of predictable and thermodynamically stable DNA nanomachines remains challenging for the monitoring of intracellular microRNAs (miRNAs) in complex biological matrices. In this study, a fluorescent biosensor utilizing a confined-free synergistic configuration is presented for the precise detection of miR-21. To mitigate the thermodynamic limitations of homogeneous catalytic hairpin assembly (CHA), a process in which unconstrained molecular diffusion induces background leakage, the amplification components were spatially partitioned. The sensing platform incorporates a rigid DNA nanosphere (NP), assembled via the annealing of three palindromic sequences, which serves as a sterically hindered scaffold to strictly confine hairpin probe 1 (HP1). Target miR-21 initiates the opening of the anchored HP1, subsequently recruiting freely diffusible auxiliary probes (HP2 and HP3) to form a localized Y-shaped complex. This reaction generates a surrogate miR-21 sequence that detaches and unlocks adjacent HP1 probes, establishing an inter-particle chemical communication network. By integrating nanoscale spatial confinement with this propagated catalytic cascade, the proposed confined-free self-circulating amplifier (CF-SCA) suppresses background leakage while maintaining rapid mass transfer, yielding a limit of detection of 100 pM. Additionally, the dense 3D nanoscaffold provides nuclease resistance. Following liposome-mediated cellular delivery, the confined-free mechanism counteracts macromolecular crowding, facilitating in situ dynamic imaging of miR-21 in living cancer cells. Evaluated through the stratification of clinical serum samples from multiple cancer cohorts, the CF-SCA nanoplatform offers an enzyme-free tool for clinical diagnostics and liquid biopsy applications.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130416"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148696759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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