IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-02-01 Epub Date: 2025-08-05 DOI: 10.1016/j.bioelechem.2025.109074

Nuttakorn Junlapak, Suntisak Khumngern, Natha Nontipichet, Tawatchai Kangkamano, Panote Thavarungkul, Atchara Lomae, Tanan Bejrananda, Apon Numnuam

{"title":"A label-free electrochemical immunosensor for bladder tumor marker NMP22 using AuNPs@OMC and Thi@Gr-COOH nanocomposites.","authors":"Nuttakorn Junlapak, Suntisak Khumngern, Natha Nontipichet, Tawatchai Kangkamano, Panote Thavarungkul, Atchara Lomae, Tanan Bejrananda, Apon Numnuam","doi":"10.1016/j.bioelechem.2025.109074","DOIUrl":"10.1016/j.bioelechem.2025.109074","url":null,"abstract":"A highly sensitive and selective label-free electrochemical immunosensor was developed to detect nuclear matrix protein 22 (NMP22), a bladder cancer marker, in urine. A screen-printed carbon electrode (SPCE) was modified with carboxylate graphene-supported thionine (Thi@Gr-COOH) as a redox probe, and a unique structure of ordered mesoporous carbon decorated with gold nanoparticles (AuNPs@OMC). The large active site and uniform porosity of OMC facilitated the deposition of AuNPs, significantly increasing the antibody coverage. NMP22 concentration was determined based on changes in the peak current of Thi reduction measured by differential pulse voltammetry before and after the formation of the immunocomplex. In the optimal condition, the proposed immunosensor demonstrated linearity of 1.0 × 10-7 to 1.0 × 10-1 ng mL-1 with detection limit of 2.96 × 10-8 ng mL-1. Furthermore, the proposed sensor demonstrated good reproducibility, stability for over 20 days, reusability up to 5 cycles of binding and regeneration, and good selectivity. The developed electrochemical immunosensor effectively detected NMP22 in human urine samples, achieving good recoveries and results that matched the NMP22™ Bladderchek™ TEST, proving it can be used effectively.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"109074"},"PeriodicalIF":4.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative magnetic bead-based electrochemical platform for rapid and sensitive cocaine detection in biological fluids. 基于磁珠的新型电化学平台，用于快速灵敏地检测生物液体中的可卡因。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-02-01 Epub Date: 2025-08-06 DOI: 10.1016/j.bioelechem.2025.109072

Mohamed Zouari, Ahmet Cetinkaya, Sibel A Ozkan

{"title":"Innovative magnetic bead-based electrochemical platform for rapid and sensitive cocaine detection in biological fluids.","authors":"Mohamed Zouari, Ahmet Cetinkaya, Sibel A Ozkan","doi":"10.1016/j.bioelechem.2025.109072","DOIUrl":"10.1016/j.bioelechem.2025.109072","url":null,"abstract":"Detecting psychoactive substances in biological samples presents significant challenges in clinical diagnostics, forensic analysis, and public health monitoring. This study introduces a highly sensitive electrochemical biosensing platform for the detection of cocaine, addressing the critical need for rapid, field-deployable testing methods. By integrating functionalized magnetic beads (MBs) with screen-printed carbon electrodes (SPCEs), we developed a competitive immunoassay system that leverages the superior molecular recognition capabilities of antibodies while maintaining operational simplicity. The biosensor operates via a competitive binding mechanism, in which cocaine present in the sample competes with cocaine-bovine serum albumin (BSA) conjugates immobilized on MBs for binding sites on horseradish peroxidase-labeled anti-cocaine antibodies (HRP-DAb). Electrochemical detection is achieved through amperometric measurement of enzyme activity using a redox system consisting of hydrogen peroxide/hydroquinone (H₂O₂/HQ). The optimized biosensor demonstrates excellent analytical performance with a linear response range from 0.3 to 300 ng mL-1 and a detection limit of 0.1 ng mL-1. Notably, the biosensor maintains its performance when analyzing cocaine in complex biological matrices, including human saliva and urine, successfully quantifying concentrations with minimal matrix interference. The platform offers significant advantages, including single-use disposable electrodes, rapid analysis time (< 30 min), minimal sample preparation requirements, and the potential for miniaturization into portable devices. These characteristics combined with high selectivity, a simple fabrication process, and cost-effectiveness, position this biosensor as a promising tool for point-of-care testing and field applications in clinical, forensic, and roadside testing scenarios.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"109072"},"PeriodicalIF":4.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal-organic framework-enhanced Electrochemiluminescence of pyrene-based ligands for sensitive CEA detection. 金属有机框架增强芘基配体电化学发光对CEA的敏感检测。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-02-01 Epub Date: 2025-08-05 DOI: 10.1016/j.bioelechem.2025.109073

Tiantian Ji, Yige Li, Mingzhe Jiang, Yingying Cheng, Haoyi Ren, Hongling Li, Chenglin Hong

{"title":"Metal-organic framework-enhanced Electrochemiluminescence of pyrene-based ligands for sensitive CEA detection.","authors":"Tiantian Ji, Yige Li, Mingzhe Jiang, Yingying Cheng, Haoyi Ren, Hongling Li, Chenglin Hong","doi":"10.1016/j.bioelechem.2025.109073","DOIUrl":"10.1016/j.bioelechem.2025.109073","url":null,"abstract":"The early and sensitive detection of cancer, a malignant disease posing significant threats to human health, is of strategic importance for disease prevention and control. This study employed 1,3,6,8-tetra(4-carboxyphenyl)pyrene (H4TBAPy), a fluorophore exhibiting aggregation-caused quenching (ACQ), to construct a zinc-based metal-organic framework (Zn-TBAPy) serving as an energy donor platform. Polydopamine-coated ZIF-67 (ZIF-67@PDA) was employed as the energy acceptor to construct an electrochemiluminescence (ECL) immunosensor for sensitive carcinoembryonic antigen (CEA) detection. The advantages of ECL immunosensor are primarily manifested in the following three aspects: (1) Zn-TBAPy not only mitigates ACQ caused by polycyclic aromatic hydrocarbon π-π stacking but also enhances chromophore loading capacity and specific surface area. Relative to aggregate systems, the Zn-TBAPy exhibits a 2.5-fold enhancement in ECL signal intensity. (2) ZIF-67@PDA exhibits favorable broad-spectrum absorption characteristics and excellent quenching efficiency; as well as demonstrates superior biocompatibility for immunosensor construction. (3) The immunosensor was constructed through an electrochemiluminescence resonance energy transfer (ECL-RET) mechanism, yielding markedly improved sensitivity; the developed sensor demonstrated a linear detection range from100 fg·mL-1 to 80 ng·mL-1with LOD) of 0.275 pg·mL-1. In conclusion, this study provides a valuable research strategy for the construction of immunosensors based on novel luminophore materials.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"109073"},"PeriodicalIF":4.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A magnetic hydrogel adsorbent bead containing hyper-crosslinked polymer-decorated graphene oxide for the dispersive liquid-solid phase extraction of phenylurea herbicides. 一种用于苯脲类除草剂分散液固萃取的超交联聚合物修饰氧化石墨烯磁性水凝胶吸附头。

IF 6.1 1区化学

Talanta Pub Date : 2026-01-01 Epub Date: 2025-07-01 DOI: 10.1016/j.talanta.2025.128538

Suppawan Sillapawisut, Piyaluk Nurerk, Opas Bunkoed

{"title":"A magnetic hydrogel adsorbent bead containing hyper-crosslinked polymer-decorated graphene oxide for the dispersive liquid-solid phase extraction of phenylurea herbicides.","authors":"Suppawan Sillapawisut, Piyaluk Nurerk, Opas Bunkoed","doi":"10.1016/j.talanta.2025.128538","DOIUrl":"10.1016/j.talanta.2025.128538","url":null,"abstract":"A porous magnetic composite adsorbent was developed by incorporating hyper-crosslinked polymer-decorated graphene oxide (Fe3O4@HCP-GO) into alginate hydrogel beads, forming Fe3O4@HCP-GO alginate beads. For the first time, this unique hierarchical structure combining magnetic responsiveness, high surface area, and multiple adsorption mechanisms was engineered for the efficient extraction of phenylurea herbicides (PUHs). The material was applied in a dispersive liquid-solid phase extraction (DLSPE) process, enabling the simultaneous adsorption of six PUHs through hydrogen bonding, hydrophobic, and π-π interactions. The extracted PUHs were determined by high performance liquid chromatography. The fabricated porous adsorbent was characterized, and the extraction conditions were optimized. Under optimal conditions, the developed method exhibited linearity from 5.0 to 100 μg L-1 for metoxuron, monuron, chlortoluron and buturon, and from 10 to 100 μg L-1 for isoproturon and monolinuron. The limits of detection were between 1.0 and 3.0 μg L-1. The developed adsorbent was utilized to extract PUHs from rice, cucumber and tomato, achieving recoveries ranging from 70.2 to 96.7 % with RSDs below 9.0 %. The fabricated porous magnetic composite hydrogel bead exhibited good stability and efficiency for up to 6 cycles of extraction and desorption.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"128538"},"PeriodicalIF":6.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Humidity sensors based on surface-functionalized tunable photonic crystal grating. 基于表面功能化可调谐光子晶体光栅的湿度传感器。

IF 6.1 1区化学

Talanta Pub Date : 2026-01-01 Epub Date: 2025-06-26 DOI: 10.1016/j.talanta.2025.128521

Hao Cui, Dingwen Hu, Tao Yang, Chan Huang, Zongyin Yang, Shurong Dong

{"title":"Humidity sensors based on surface-functionalized tunable photonic crystal grating.","authors":"Hao Cui, Dingwen Hu, Tao Yang, Chan Huang, Zongyin Yang, Shurong Dong","doi":"10.1016/j.talanta.2025.128521","DOIUrl":"10.1016/j.talanta.2025.128521","url":null,"abstract":"Photonic crystal (PC)-based humidity sensors detect changes in humidity using periodic structural color variations and have significant potential in the humidity detection field. However, current technologies typically rely on observing these structural color changes with the human eye. The human eye has limited color discrimination, thus resulting in insufficient detection accuracy. Meanwhile, viewing angles and ambient lighting can also disrupt observations. Here, we propose a humidity sensor based on surface-functionalized tunable PC grating. The tunable PC grating consists of a 600 nm polystyrene (PS) microsphere PC and a humidity-sensitive hydrogel. As ambient humidity increases, the hydrophilic amide groups (-CONH2) inside the hydrogel interact with the hydrogen bonds between water molecules and triggers hydrogel swelling, exerts interfacial stress on the PS microsphere lattice, thus expanding the lattice spacing of the PS microspheres and causing a red shift in the reflected wavelength. Integrating the surface-functionalized tunable PC grating into a Czerny-Turner (C-T) optical system enables us to directly translate humidity into precise spectral shifts, overcoming the limitations of human eye-based observations. Experimental results demonstrate a strong linear response over the range of 24-94 % relative humidity (RH), as well as excellent repeatability and long-term stability. We provide an innovative solution for high-precision optical humidity sensing.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"128521"},"PeriodicalIF":6.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functionalized magnetic nanoprobe-based aptasensor for on-site detection of kanamycin in water by a personal glucose meter. 基于功能化磁性纳米探针的适体传感器用于个人血糖仪对水中卡那霉素的现场检测。

IF 6.1 1区化学

Talanta Pub Date : 2026-01-01 Epub Date: 2025-06-24 DOI: 10.1016/j.talanta.2025.128515

Ting Lei, Chengyan Yin, Hui Feng, Xiangwen Lei, Ruth Antwi-Baah, Jing Liu, Li Zhang, Wulin Yang, Shuxia Xu

{"title":"Functionalized magnetic nanoprobe-based aptasensor for on-site detection of kanamycin in water by a personal glucose meter.","authors":"Ting Lei, Chengyan Yin, Hui Feng, Xiangwen Lei, Ruth Antwi-Baah, Jing Liu, Li Zhang, Wulin Yang, Shuxia Xu","doi":"10.1016/j.talanta.2025.128515","DOIUrl":"10.1016/j.talanta.2025.128515","url":null,"abstract":"In this work, the nanomagnetic probe was prepared by condensation of carboxylated kanamycin (Kana) aptamers with aminated nanomagnetic Fe3O4 beads, which was coupled with invertase-labeled complementary DNA (cDNA) for sensitive and convenient detection of Kana in water samples by a personal glucose meter (PGM). In the absence of Kana, there was no PGM signal recorded because formed nanomagnetic probe was removed by magnetic separation. Conversely, the aptamer could recognize Kana specifically and released cDNA into supernatant. After the addition of sucrose, it was hydrolyzed into glucose by invertase. Detection of Kana was indirectly achieved based on the relationship between glucose concentration and the PGM indication. Furthermore, a miniaturized portable device was constructed by integrating the components using 3D printing technology. Under optimal conditions, rapid detection of Kana within the concentration range of 1-200 nM was achieved, with a detection limit (3σ/k) of 0.28 nM. Moreover, the proposed aptasensor was characterized in terms of selectivity, reproducibility and stability. Finally, it was applied to the determination of Kana in water with recoveries from 99% to 103%. All these results indicated that the proposed aptasensor is simple, rapid, selective, and sensitive, and it could potentially serve as an effective approach for the on-site determination of Kana in water samples.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"128515"},"PeriodicalIF":6.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid determination of biogenic amines in meats using quinone chemistry-based colorimetric strategy. 用醌化学比色法快速测定肉类中的生物胺。

IF 6.1 1区化学

Talanta Pub Date : 2026-01-01 Epub Date: 2025-06-27 DOI: 10.1016/j.talanta.2025.128527

Kaiying Hu, Shuai Liu, Yang Han, Jingxiang Sun, Yu Mao, Hao Qu, Baolei Dong, Lei Zheng

{"title":"Rapid determination of biogenic amines in meats using quinone chemistry-based colorimetric strategy.","authors":"Kaiying Hu, Shuai Liu, Yang Han, Jingxiang Sun, Yu Mao, Hao Qu, Baolei Dong, Lei Zheng","doi":"10.1016/j.talanta.2025.128527","DOIUrl":"10.1016/j.talanta.2025.128527","url":null,"abstract":"Rapid and convenient detection of biogenic amines (BAs) in meat products is a vital approach for ensuring food quality and consumer health. In this study, we developed a quinone chemistry-mediated colorimetric strategy for the rapid detection of BAs in meat. BAs such as putrescine undergo a visible colorimetric reaction with 2-methyl-1,4-benzoquinone (MBQ), producing a product with strong UV-visible absorption at 480 nm, enabling specific colorimetric detection of BAs. Structural analysis of the chromogenic product indicated that a Michael addition reaction between BAs and MBQ was the primary mechanism driving the color change. A deep eutectic solvent (DES) was employed to achieve mild and efficient extraction of BAs from samples. Combined with the quinone-mediated colorimetric reaction, we developed a rapid colorimetric detection method for BAs in pork, fish, and shrimp. The results of the colorimetric assay showed good agreement with those obtained from the Kjeldahl-based nitrogen determination method. The developed method does not rely on photosensitive dyes/pigments or structurally complex nanomaterials, offering a simple, efficient, and low-cost strategy for the rapid quantification of BAs and freshness monitoring in meat products.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"128527"},"PeriodicalIF":6.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of arsenic species in seafood samples using a combination of HPLC and ICP-MS after ultrasonic assisted sample preparation. 超声辅助制样后HPLC - ICP-MS联合测定海产品样品中砷的种类。

IF 6.1 1区化学

Talanta Pub Date : 2026-01-01 Epub Date: 2025-06-27 DOI: 10.1016/j.talanta.2025.128518

Sabriye Sel, Büşra Aydoğan, İkbal Koyuncu

{"title":"Determination of arsenic species in seafood samples using a combination of HPLC and ICP-MS after ultrasonic assisted sample preparation.","authors":"Sabriye Sel, Büşra Aydoğan, İkbal Koyuncu","doi":"10.1016/j.talanta.2025.128518","DOIUrl":"10.1016/j.talanta.2025.128518","url":null,"abstract":"In this study, an analytical method was applied for the separation and determination of inorganic and organic arsenic species (As+3, As+5, AsB, MMA) in anchovy, haddock, mussels and prawns using HPLC coupled with ICP-MS. Efficient separation of the arsenic species on the HPLC-ICP-MS system was achieved using 50 mM (NH4)2CO3 at pH 9.50 diluted in 1 % methanol and 0.50 mM EDTA as mobile phase. The arsenic species were extracted from the samples by means of ultrasonically assisted sample preparation. The LOD/LOQ values of the detection system were 0.07/0.22 ng/mL, 0.12/0.41 ng/mL, 0.06/0.22 ng/mL and 0.03/0.11 ng/mL for arsenobetaine (AsB), As+3, methylarsonic acid (MMA) and As+5, respectively. The relative standard deviations calculated for the lowest calibration standards ranged between 1.01 and 7.88 %, verifying good precision for replicate measurements. The accuracy of the method was validated by spike recovery experiments, with recorded recoveries in the range of 85-117 %.The total arsenic content of the extracts was determined by direct ICP-MS analysis. A certificated reference material (NIST-1573A) was analyzed for the total As concentration. The method was successfully applied for the qualitative and quantitative determination of AsB, As+3, MMA and As+5 in the seafood samples ranging from 6.0 to 5700 ng/g. The carcinogenic and non-carcinogenic risk for haddock and anchovies was low, whereas the carcinogenic risk for prawns was relatively high but below the threshold value, according to the risk assessment of the samples.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"128518"},"PeriodicalIF":6.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile design and synthesis of boric acid group based fluorescent probes for monitoring mitochondrial ATP fluctuation. 基于硼酸基荧光探针监测线粒体ATP波动的简易设计与合成。

IF 6.1 1区化学

Talanta Pub Date : 2026-01-01 Epub Date: 2025-06-27 DOI: 10.1016/j.talanta.2025.128526

Bin Yang, Jiao Lu, Zhou Wu, Yong Li, Jun Wang, Ruihong Yao, Yanli Li, Zhihao Lu, Zhirong Geng, Zhilin Wang

{"title":"Facile design and synthesis of boric acid group based fluorescent probes for monitoring mitochondrial ATP fluctuation.","authors":"Bin Yang, Jiao Lu, Zhou Wu, Yong Li, Jun Wang, Ruihong Yao, Yanli Li, Zhihao Lu, Zhirong Geng, Zhilin Wang","doi":"10.1016/j.talanta.2025.128526","DOIUrl":"10.1016/j.talanta.2025.128526","url":null,"abstract":"Adenosine 5'-triphosphate (ATP) is a high-energy phosphate compound that provides most of the energy needed for the physiological activities of organisms. The abnormal fluctuation of intracellular ATP level is closely related to a variety of physiological diseases. So, the monitoring of ATP levels is of great significance for the diagnosis and treatment of diseases. Herein, tetraphenylethylene and triphenylamine fluorophores were employed to synthesize six fluorescent probes with different structures, including TPE-P1, TPE-P2, TPE-P3, TPA-P4, TPA-P5 and TPA-P6. These probes all exhibited the advantages of long-wavelength emission, good biocompatibility and large Stokes shifts. Notably, the nitrogen-containing positively charged site and boric acid group of these probes could be used as ATP recognition groups. Then, the selectivity experiments indicated that TPA-P4 possessed good selectivity and higher fluorescence enhancement degree for ATP compared with the other probes, and was more suitable for detection of ATP. Further, TPA-P4 was successfully applied to the imaging detection of ATP in mitochondria. Importantly, TPA-P4 could also distinguish between normal liver cells (LO2) and liver cancer cells (HepG2) by imaging ATP, which may show great potential applications in tumor diagnosis. It had also been successfully applied to the detection of ATP levels during liver injury and inflammation, which is expected to provide important information for the diagnosis and treatment of diseases in the future.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"128526"},"PeriodicalIF":6.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of dual detection platforms based on catalytic hairpin assembly for rapid and sensitive detection of Chlamydia trachomatis 16S rRNA. 基于催化发夹组件的快速灵敏沙眼衣原体16S rRNA双检测平台的开发

IF 6.1 1区化学

Talanta Pub Date : 2026-01-01 Epub Date: 2025-06-20 DOI: 10.1016/j.talanta.2025.128495

Dawen Chen, Shuo Ma, Jiwei Wang, Yuming Yao, Yaya Chen, Gulinaizhaer Abudushalamu, Meiling Zhou, Ai Chen, Chenyan Yuan, Xun Gao, Xiaobo Fan, Chen Zhang, Guoqiu Wu

{"title":"Development of dual detection platforms based on catalytic hairpin assembly for rapid and sensitive detection of Chlamydia trachomatis 16S rRNA.","authors":"Dawen Chen, Shuo Ma, Jiwei Wang, Yuming Yao, Yaya Chen, Gulinaizhaer Abudushalamu, Meiling Zhou, Ai Chen, Chenyan Yuan, Xun Gao, Xiaobo Fan, Chen Zhang, Guoqiu Wu","doi":"10.1016/j.talanta.2025.128495","DOIUrl":"10.1016/j.talanta.2025.128495","url":null,"abstract":"Chlamydia trachomatis (CT) infections often remain asymptomatic yet can cause severe complications. Although nucleic acid amplification tests (NAATs) offer high sensitivity, they require expensive equipment unavailable in resource-limited settings. We developed the first application of catalytic hairpin assembly (CHA) technology combined with fluorescence immunochromatography assay (FICA) and colloidal gold immunochromatography assay (GICA) for CT 16S rRNA detection. We optimized reaction conditions (temperature, pH, probe ratio, and concentration) to minimize background signals and ensure CHA reaction feasibility. Using RT-qPCR as reference standard, we evaluated 38 CT-positive and 62 CT-negative clinical vaginal swab specimens. CHA-FICA achieved a detection limit of 10 fM with 89.47 % sensitivity and 100 % specificity within 25 min. CHA-GICA reached a detection limit of 1 pM with 81.57 % sensitivity and 100 % specificity within 30 min. Both platforms showed excellent concordance with RT-qPCR results: CHA-FICA achieved 96 % accuracy (AUC = 0.988) and CHA-GICA 93 % accuracy (AUC = 0.979). The probes remained stable for up to 5 weeks at -20 °C. This cost-effective nucleic acid detection strategy is suitable for resource-limited settings, providing both theoretical insights and practical tools for early CT diagnosis.","PeriodicalId":435,"journal":{"name":"Talanta","volume":"296 ","pages":"128495"},"PeriodicalIF":6.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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