Electroanalysis最新文献

Preparation of TEMPO-Modified Electrodes by Electrochemical Polymerization in Aqueous Solution for Use in Electrochemical Analysis of Pharmaceuticals 水溶液电化学聚合法制备用于药物电化学分析的tempo修饰电极

IF 2.3 3区化学

Electroanalysis Pub Date : 2025-10-10 DOI: 10.1002/elan.70047

Ryota Imahashi, Kyoko Sugiyama, Fumiya Sato, Tetsuya Ono, Kentaro Yoshida, Ayumi Kirii, Kazuhiro Watanabe, Hiroaki Yamaguchi, Katsuhiko Sato, Yoshitomo Kashiwagi

{"title":"Preparation of TEMPO-Modified Electrodes by Electrochemical Polymerization in Aqueous Solution for Use in Electrochemical Analysis of Pharmaceuticals","authors":"Ryota Imahashi, Kyoko Sugiyama, Fumiya Sato, Tetsuya Ono, Kentaro Yoshida, Ayumi Kirii, Kazuhiro Watanabe, Hiroaki Yamaguchi, Katsuhiko Sato, Yoshitomo Kashiwagi","doi":"10.1002/elan.70047","DOIUrl":"https://doi.org/10.1002/elan.70047","url":null,"abstract":"In this study, we evaluated the preparation conditions, stability, and durability of 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO)-modified glassy carbon (GC) electrodes prepared using electrolytic polymerization in an aqueous solvent, and examined the applicability of the electrodes as electrochemical sensors for pharmaceutical analysis. First, we synthesized a TEMPO-substituted phenol (2-PH-TEMPO) and examined the conditions for its efficient immobilization on an electrode using the electrolytic polymerization method in an aqueous solvent. The TEMPO-modified GC electrode showed high stability and durability in a low-concentration substrate environment and effectively functioned as an electrolytic oxidation catalyst for ethanol, a compound with a hydroxyl group. In an analysis of pharmaceuticals, a high oxidative response was obtained for lidocaine, clarithromycin, and azithromycin, which have tertiary amines or multiple oxidizable hydroxyl groups, suggesting that the electrode can function as a highly sensitive electrochemical sensor. The use of this modified electrode enables quantification in the clinical concentration range of vancomycin, and it is expected to be applied to rapid and accurate pharmaceutical concentration measurements in therapeutic drug monitoring. These results suggest that TEMPO-modified GC electrodes are useful as stable and sensitive electrochemical sensors in the fields of pharmaceutical analysis and biosensors, and are expected to contribute to the development of quantitative electrochemical methods.","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145272299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of TEMPO-Modified Electrodes by Electrochemical Polymerization in Aqueous Solution for Use in Electrochemical Analysis of Pharmaceuticals 水溶液电化学聚合法制备用于药物电化学分析的tempo修饰电极

IF 2.3 3区化学

Electroanalysis Pub Date : 2025-10-10 DOI: 10.1002/elan.70047

Ryota Imahashi, Kyoko Sugiyama, Fumiya Sato, Tetsuya Ono, Kentaro Yoshida, Ayumi Kirii, Kazuhiro Watanabe, Hiroaki Yamaguchi, Katsuhiko Sato, Yoshitomo Kashiwagi

{"title":"Preparation of TEMPO-Modified Electrodes by Electrochemical Polymerization in Aqueous Solution for Use in Electrochemical Analysis of Pharmaceuticals","authors":"Ryota Imahashi, Kyoko Sugiyama, Fumiya Sato, Tetsuya Ono, Kentaro Yoshida, Ayumi Kirii, Kazuhiro Watanabe, Hiroaki Yamaguchi, Katsuhiko Sato, Yoshitomo Kashiwagi","doi":"10.1002/elan.70047","DOIUrl":"https://doi.org/10.1002/elan.70047","url":null,"abstract":"In this study, we evaluated the preparation conditions, stability, and durability of 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO)-modified glassy carbon (GC) electrodes prepared using electrolytic polymerization in an aqueous solvent, and examined the applicability of the electrodes as electrochemical sensors for pharmaceutical analysis. First, we synthesized a TEMPO-substituted phenol (2-PH-TEMPO) and examined the conditions for its efficient immobilization on an electrode using the electrolytic polymerization method in an aqueous solvent. The TEMPO-modified GC electrode showed high stability and durability in a low-concentration substrate environment and effectively functioned as an electrolytic oxidation catalyst for ethanol, a compound with a hydroxyl group. In an analysis of pharmaceuticals, a high oxidative response was obtained for lidocaine, clarithromycin, and azithromycin, which have tertiary amines or multiple oxidizable hydroxyl groups, suggesting that the electrode can function as a highly sensitive electrochemical sensor. The use of this modified electrode enables quantification in the clinical concentration range of vancomycin, and it is expected to be applied to rapid and accurate pharmaceutical concentration measurements in therapeutic drug monitoring. These results suggest that TEMPO-modified GC electrodes are useful as stable and sensitive electrochemical sensors in the fields of pharmaceutical analysis and biosensors, and are expected to contribute to the development of quantitative electrochemical methods.","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145272513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct and Reagent-Free Fabrication of a Laser-Induced-Graphene-Gold Nanocomposite Electrode From Gold Leaves Using a Do-It-Yourself Blue Laser System 使用自制蓝光激光系统直接和无试剂制备激光诱导石墨烯-金纳米复合电极

IF 2.3 3区化学

Electroanalysis Pub Date : 2025-10-07 DOI: 10.1002/elan.70068

Kazuki Umetsu, Naoki Narisawa, Yutaro Harada, Kenta Takano, Shinnosuke Harada, Keita Mitsui, Shoi Harada, Haruya Okimoto, Hiroya Abe, Kuniaki Nagamine

引用次数: 0

Alternating Current-Driven Electrochromic Devices Based on Benzyl Viologen: AC Frequency Indicator 基于苄基紫胶的交流驱动电致变色器件：交流频率指示器

IF 2.3 3区化学

Electroanalysis Pub Date : 2025-10-07 DOI: 10.1002/elan.70063

Weiyi Chen, Meng Wang, Shijie Xu, Lu Li, Xiaodi Liu, Debao Xiao

引用次数: 0

Design and Optimization of Biosensor for Pyruvate Quantification 丙酮酸定量生物传感器的设计与优化

IF 2.3 3区化学

Electroanalysis Pub Date : 2025-10-07 DOI: 10.1002/elan.70064

Yevhen Vakhovskyі, Daryna Mruga, Oleksii Ustinov, Aldo Di Costanzo Mata, Oleksandr Soldatkin, Serhii Dzyadevych

{"title":"Design and Optimization of Biosensor for Pyruvate Quantification","authors":"Yevhen Vakhovskyі, Daryna Mruga, Oleksii Ustinov, Aldo Di Costanzo Mata, Oleksandr Soldatkin, Serhii Dzyadevych","doi":"10.1002/elan.70064","DOIUrl":"https://doi.org/10.1002/elan.70064","url":null,"abstract":"In this work, an amperometric pyruvate-sensitive biosensor was developed and optimized. A platinum disk electrode was used as an amperometric transducer. The bioselective element of the biosensor is based on the pyruvate oxidase enzyme immobilized with PVA-SbQ polymer by photopolymerization. The optimal immobilization conditions (enzyme and photopolymer concentrations, duration, and intensity of photopolymerization) were selected. The optimal concentrations of cofactors and cosubstrates (phosphate ions, thiamine pyrophosphate, and magnesium ions (Mg2+) were chosen to ensure the best sensitivity of the developed biosensor to pyruvate. After optimizing the design of the bioselective element and the immobilization process, the reproducibility of the biosensor manufacturing procedure was investigated (RSD = 12.5%). To assess the prospects of the developed biosensor, its main analytical characteristics were analyzed. The linear range of the biosensor was from 10 to 500 μM pyruvate, and the sensitivity was 66 nA/mM. The minimum detection threshold was 1.57 μM pyruvate. The obtained data indicate the technical feasibility and prospects of using the developed biosensor for determining the concentration of pyruvate in real multicomponent biological samples.","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145271998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alternating Current-Driven Electrochromic Devices Based on Benzyl Viologen: AC Frequency Indicator 基于苄基紫胶的交流驱动电致变色器件：交流频率指示器

IF 2.3 3区化学

Electroanalysis Pub Date : 2025-10-07 DOI: 10.1002/elan.70063

Weiyi Chen, Meng Wang, Shijie Xu, Lu Li, Xiaodi Liu, Debao Xiao

引用次数: 0

Design and Optimization of Biosensor for Pyruvate Quantification 丙酮酸定量生物传感器的设计与优化

IF 2.3 3区化学

Electroanalysis Pub Date : 2025-10-07 DOI: 10.1002/elan.70064

Yevhen Vakhovskyі, Daryna Mruga, Oleksii Ustinov, Aldo Di Costanzo Mata, Oleksandr Soldatkin, Serhii Dzyadevych

{"title":"Design and Optimization of Biosensor for Pyruvate Quantification","authors":"Yevhen Vakhovskyі, Daryna Mruga, Oleksii Ustinov, Aldo Di Costanzo Mata, Oleksandr Soldatkin, Serhii Dzyadevych","doi":"10.1002/elan.70064","DOIUrl":"https://doi.org/10.1002/elan.70064","url":null,"abstract":"In this work, an amperometric pyruvate-sensitive biosensor was developed and optimized. A platinum disk electrode was used as an amperometric transducer. The bioselective element of the biosensor is based on the pyruvate oxidase enzyme immobilized with PVA-SbQ polymer by photopolymerization. The optimal immobilization conditions (enzyme and photopolymer concentrations, duration, and intensity of photopolymerization) were selected. The optimal concentrations of cofactors and cosubstrates (phosphate ions, thiamine pyrophosphate, and magnesium ions (Mg2+) were chosen to ensure the best sensitivity of the developed biosensor to pyruvate. After optimizing the design of the bioselective element and the immobilization process, the reproducibility of the biosensor manufacturing procedure was investigated (RSD = 12.5%). To assess the prospects of the developed biosensor, its main analytical characteristics were analyzed. The linear range of the biosensor was from 10 to 500 μM pyruvate, and the sensitivity was 66 nA/mM. The minimum detection threshold was 1.57 μM pyruvate. The obtained data indicate the technical feasibility and prospects of using the developed biosensor for determining the concentration of pyruvate in real multicomponent biological samples.","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145272052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct and Reagent-Free Fabrication of a Laser-Induced-Graphene-Gold Nanocomposite Electrode From Gold Leaves Using a Do-It-Yourself Blue Laser System 使用自制蓝光激光系统直接和无试剂制备激光诱导石墨烯-金纳米复合电极

IF 2.3 3区化学

Electroanalysis Pub Date : 2025-10-07 DOI: 10.1002/elan.70068

Kazuki Umetsu, Naoki Narisawa, Yutaro Harada, Kenta Takano, Shinnosuke Harada, Keita Mitsui, Shoi Harada, Haruya Okimoto, Hiroya Abe, Kuniaki Nagamine

引用次数: 0

Nanostructured Biosensor Based on Multiwalled Carbon Nanotubes and Antimicrobial Peptide Temporin-PTA for Bacterial Detection 基于多壁碳纳米管和抗菌肽Temporin-PTA的细菌检测纳米结构生物传感器

IF 2.3 3区化学

Electroanalysis Pub Date : 2025-09-30 DOI: 10.1002/elan.70065

Antônio Oscar Gomes Filho, Alberto Galdino da Silva Júnior, Reginaldo G. Lima-Neto, Ludovico Migliolo, Maria Danielly Lima de Oliveira, César Augusto Souza de Andrade

{"title":"Nanostructured Biosensor Based on Multiwalled Carbon Nanotubes and Antimicrobial Peptide Temporin-PTA for Bacterial Detection","authors":"Antônio Oscar Gomes Filho, Alberto Galdino da Silva Júnior, Reginaldo G. Lima-Neto, Ludovico Migliolo, Maria Danielly Lima de Oliveira, César Augusto Souza de Andrade","doi":"10.1002/elan.70065","DOIUrl":"https://doi.org/10.1002/elan.70065","url":null,"abstract":"Bacterial infections represent a major public health challenge due to treatment difficulties and resistance spread. Antimicrobial peptides (AMPs) offer innovative applications in biosensors, since their interaction with microbial membranes can be detected by electrochemical changes. This study developed a nanostructured sensor using multiwalled carbon nanotubes (MWCNTs) and the antimicrobial peptide Temporin-PTA (T-PTA), derived from Hylarana picturata skin secretion. MWCNTs were electrodeposited on electrodes via cyclic voltammetry (CV) in acidic dispersion, improving electron kinetics and enabling chemical immobilization of T-PTA. The system was applied to detect Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus in a label-free electrochemical assay. Electrochemical impedance spectroscopy (EIS) and CV confirmed sensor assembly and interaction with different bacterial concentrations. Complementary analyses with atomic force microscopy (AFM) and Fourier-transform infrared spectroscopy (FTIR) evaluated gradual adhesion of platform components. The biosensor detected concentrations from 101 to 105 CFU/mL within only 5 min. Notably, the electrochemical signal was stronger for Gram-negative bacteria, particularly P. aeruginosa, consistent with T-PTA's affinity for electronegative surfaces. This system demonstrated rapid, sensitive, and selective detection, distinguishing Gram-negative from Gram-positive species. Such characteristics highlight its potential as a valuable complement to gold-standard microbiological methods.","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elan.70065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanostructured Biosensor Based on Multiwalled Carbon Nanotubes and Antimicrobial Peptide Temporin-PTA for Bacterial Detection 基于多壁碳纳米管和抗菌肽Temporin-PTA的细菌检测纳米结构生物传感器

IF 2.3 3区化学

Electroanalysis Pub Date : 2025-09-30 DOI: 10.1002/elan.70065

Antônio Oscar Gomes Filho, Alberto Galdino da Silva Júnior, Reginaldo G. Lima-Neto, Ludovico Migliolo, Maria Danielly Lima de Oliveira, César Augusto Souza de Andrade

{"title":"Nanostructured Biosensor Based on Multiwalled Carbon Nanotubes and Antimicrobial Peptide Temporin-PTA for Bacterial Detection","authors":"Antônio Oscar Gomes Filho, Alberto Galdino da Silva Júnior, Reginaldo G. Lima-Neto, Ludovico Migliolo, Maria Danielly Lima de Oliveira, César Augusto Souza de Andrade","doi":"10.1002/elan.70065","DOIUrl":"https://doi.org/10.1002/elan.70065","url":null,"abstract":"Bacterial infections represent a major public health challenge due to treatment difficulties and resistance spread. Antimicrobial peptides (AMPs) offer innovative applications in biosensors, since their interaction with microbial membranes can be detected by electrochemical changes. This study developed a nanostructured sensor using multiwalled carbon nanotubes (MWCNTs) and the antimicrobial peptide Temporin-PTA (T-PTA), derived from Hylarana picturata skin secretion. MWCNTs were electrodeposited on electrodes via cyclic voltammetry (CV) in acidic dispersion, improving electron kinetics and enabling chemical immobilization of T-PTA. The system was applied to detect Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus in a label-free electrochemical assay. Electrochemical impedance spectroscopy (EIS) and CV confirmed sensor assembly and interaction with different bacterial concentrations. Complementary analyses with atomic force microscopy (AFM) and Fourier-transform infrared spectroscopy (FTIR) evaluated gradual adhesion of platform components. The biosensor detected concentrations from 101 to 105 CFU/mL within only 5 min. Notably, the electrochemical signal was stronger for Gram-negative bacteria, particularly P. aeruginosa, consistent with T-PTA's affinity for electronegative surfaces. This system demonstrated rapid, sensitive, and selective detection, distinguishing Gram-negative from Gram-positive species. Such characteristics highlight its potential as a valuable complement to gold-standard microbiological methods.","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elan.70065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0