{"title":"Dual Amplification Strategy: Exponential Amplification Reaction and Hybridization Chain Reaction-Driven Electrochemical Biosensor for Ultrasensitive Pathogen Detection","authors":"Shalini Devi K. S., Subbiah Alwarappan","doi":"10.1002/elan.70077","DOIUrl":"10.1002/elan.70077","url":null,"abstract":"<p>The growth of nucleic acid-based diagnostics integrated with electrochemical approaches has fundamentally transformed pathogen detection paradigms, with exponential amplification reaction (EXPAR) and hybridization chain reaction (HCR) emerging as next-generation alternatives to conventional polymerase chain reaction methodologies. EXPAR and HCR have emerged as powerful tools, offering unique biochemical pathways to amplify detection signals for pathogen-derived deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). The key interest in this amplification strategy is due to improved detection signals without the use of sophisticated thermocycling equipment. EXPAR is a potent isothermal amplification technique that employs a mix of polymerases and nicking (specific single-strand breaks) enzymes to rapidly cleave and extend short oligonucleotide sequences and, thereby it results in an exponential growth in the target DNA or RNA. This method is a perfect fit for field-based and point-of-care applications, as it enables extremely quick detection. HCR, on the other hand, employs a regulated, enzyme-free process in which metastable DNA hairpins self-assemble upon hybridization with a target sequence, and resulting in an amplified signal. HCR's programmability and flexibility make it easier to integrate into microfluidic systems and biosensors for real-time pathogen surveillance. Comparative studies suggest that EXPAR generally surpasses HCR in speed and sensitivity, whereas HCR excels in robustness, modularity, and ease of coupling with nanomaterials or aptamer-functionalized probes. This review explores the fundamental principles of EXPAR and HCR, compares their efficiency, sensitivity, and applications in pathogen diagnostics, and discusses their integration with emerging electrochemical sensing technologies. Moreover, this review elaborates on the advances in the use of nanomaterials, aptamer-functionalized probes, and the challenges of multiplexed detection strategies that further enhance their diagnostic potential. Finally, this review provides insights into future directions in next-generation pathogen detection platforms, with an emphasis on their role in the design of rapid, low-cost, and field-deployable diagnostic assays.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145572603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ratio Electrochemical Detection of Nitrofurazone Based on Carbon Nanotubes/Electroactive Schiff Base Polymer","authors":"Xiaomin Zhang, Jianhui Xiong, Wenxiao Jin, Likang Wu, Canwei Peng, Fugang Xu, Longfei Miao","doi":"10.1002/elan.70080","DOIUrl":"https://doi.org/10.1002/elan.70080","url":null,"abstract":"<p>Nitrofurazone (NFZ), as an antibiotic, is carcinogenic upon prolonged exposure. Thus, detecting NFZ and its metabolites is essential for ensuring food safety and preventing potential health risks to humans. A dual-signal electrochemical sensor consisting of a composite of carbon nanotubes and an electroactive Schiff base polymer (SBP<sub>Thi-DHA</sub>, synthesized from thionine and 2,5-dihydroxyterephthalaldehyde)/carbon nanotubes) enabled highly sensitive detection of nitrofurazolidone (NFZ). The sensor was fabricated using 2,5-dihydroxyterephthalaldehyde (DHA) and electroactive thionine (Thi) monomer as precursors to prepare spherical SBP<sub>Thi-DHA</sub> nanomaterials with an average diameter of approximately 2 μm through a Schiff base reaction. The resulting material possessed high surface area, superior electron transport capability, and abundant surface functional groups, which enabled uniform loading of amino-functionalized carbon nanotubes (NH<sub>2</sub>-CNT) and significantly enhanced the catalytic performance and detection sensitivity of the sensor. Empirical evidence demonstrated that the ratiometric electrochemical sensor achieved a remarkably low detection of 4.3 nM for NFZ at a reference signal of −0.6 V, with wide linear ranges of 13.1 nM-80 and 80–400 μM. The introduction of ratiometric signals effectively minimized interference caused by electrode surface state variations, electrolyte concentration fluctuations, and temperature changes during detection, significantly improving measurement accuracy and reproducibility. Furthermore, the excellent stability of SBP<sub>Thi-DHA</sub>/CNT on the electrode surface endowed the sensor with outstanding reusability.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145581307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ratio Electrochemical Detection of Nitrofurazone Based on Carbon Nanotubes/Electroactive Schiff Base Polymer","authors":"Xiaomin Zhang, Jianhui Xiong, Wenxiao Jin, Likang Wu, Canwei Peng, Fugang Xu, Longfei Miao","doi":"10.1002/elan.70080","DOIUrl":"https://doi.org/10.1002/elan.70080","url":null,"abstract":"<p>Nitrofurazone (NFZ), as an antibiotic, is carcinogenic upon prolonged exposure. Thus, detecting NFZ and its metabolites is essential for ensuring food safety and preventing potential health risks to humans. A dual-signal electrochemical sensor consisting of a composite of carbon nanotubes and an electroactive Schiff base polymer (SBP<sub>Thi-DHA</sub>, synthesized from thionine and 2,5-dihydroxyterephthalaldehyde)/carbon nanotubes) enabled highly sensitive detection of nitrofurazolidone (NFZ). The sensor was fabricated using 2,5-dihydroxyterephthalaldehyde (DHA) and electroactive thionine (Thi) monomer as precursors to prepare spherical SBP<sub>Thi-DHA</sub> nanomaterials with an average diameter of approximately 2 μm through a Schiff base reaction. The resulting material possessed high surface area, superior electron transport capability, and abundant surface functional groups, which enabled uniform loading of amino-functionalized carbon nanotubes (NH<sub>2</sub>-CNT) and significantly enhanced the catalytic performance and detection sensitivity of the sensor. Empirical evidence demonstrated that the ratiometric electrochemical sensor achieved a remarkably low detection of 4.3 nM for NFZ at a reference signal of −0.6 V, with wide linear ranges of 13.1 nM-80 and 80–400 μM. The introduction of ratiometric signals effectively minimized interference caused by electrode surface state variations, electrolyte concentration fluctuations, and temperature changes during detection, significantly improving measurement accuracy and reproducibility. Furthermore, the excellent stability of SBP<sub>Thi-DHA</sub>/CNT on the electrode surface endowed the sensor with outstanding reusability.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145581378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jinpeng Liu, Peigang Han, Jialin Chu, Zihan Wang, Xianling Wang, Pan Li, Huan Wang
{"title":"Synthesis of Coffee Grounds-Derived Nitrogen-Doped Porous Carbon Layer@Cobalt–Iron Alloy for Green and Economic Hydrogen Evolution","authors":"Jinpeng Liu, Peigang Han, Jialin Chu, Zihan Wang, Xianling Wang, Pan Li, Huan Wang","doi":"10.1002/elan.70074","DOIUrl":"10.1002/elan.70074","url":null,"abstract":"<p>In this study, a novel nitrogen-doped porous biomass-derived carbon layer@cobalt–iron alloy material (NPCL@Co-Fe) was successfully fabricated through a simple and eco-friendly approach. Specifically, waste biomass-coffee grounds were utilized as the raw material, and their organic components were transformed into carbon materials with a porous layered structure via high-temperature calcination. Subsequently, by exploiting the interaction between polypyrrole and cobalt-iron metal ions, and in combination with the dimethylimidazole ligand, metal-organic frameworks (MOFs) were loaded onto the porous carbon layer to form a porous carbon layer@polypyrrole@cobalt-iron MOFs composite precursor. Eventually, through high-temperature calcination of the precursor, uniform dispersion of metal nanoparticles and effective doping of nitrogen were accomplished, yielding the target material NPCL@Co-Fe. Owing to the abundant defect sites and high specific surface area of the porous carbon layer, as well as the unique electronic structure and excellent electrocatalytic performance of the cobalt-iron bimetal, the synergy between the two significantly enhanced the overall electrocatalytic activity and stability of the composite material. Experimental results indicated that this material displayed outstanding catalytic performance for the hydrogen evolution reaction (HER). The Tafel slope of the NPCL@Co<sub>1</sub>-Fe<sub>1</sub> is 117.0 mV/dec. This work provides an eco-friendly and economical idea for the synthesis of nitrogen-doped carbon materials supported nonprecious metal catalysts and achieves a relatively good electrocatalytic performance for HER to solve the problems of cumbersome steps and high preparation costs.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145572217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
José Guilherme Aquino Rodrigues, Tárcila Mathiasso Nascimento da Silva, Sidnei de Barros Gomes Junior, Antônio Augusto Lopes Marins, Victor Magno Paiva, Isabella Oliveira Britto, Gabriel Fernandes Souza dos Santos, Jairo Pinto de Oliveira, Rafael de Queiroz Ferreira, Natasha Midori Suguihiro, Rogério Valentim Gelamo, Emerson Schwingel Ribeiro, Eliane D’Elia
{"title":"Application of CO2-Plasma Treated Multilayer Graphene as an Electrochemical Sensing Platform for Paracetamol Monitoring","authors":"José Guilherme Aquino Rodrigues, Tárcila Mathiasso Nascimento da Silva, Sidnei de Barros Gomes Junior, Antônio Augusto Lopes Marins, Victor Magno Paiva, Isabella Oliveira Britto, Gabriel Fernandes Souza dos Santos, Jairo Pinto de Oliveira, Rafael de Queiroz Ferreira, Natasha Midori Suguihiro, Rogério Valentim Gelamo, Emerson Schwingel Ribeiro, Eliane D’Elia","doi":"10.1002/elan.70078","DOIUrl":"https://doi.org/10.1002/elan.70078","url":null,"abstract":"<p>Developing advanced materials is crucial for improving electrochemical sensing platforms, particularly by enhancing sensitivity, selectivity, and miniaturization. In this study, we introduce a CO<sub>2</sub> plasma-treated multilayer graphene (MLG) paper as a novel electrode material for the electrochemical detection of paracetamol (PAR). A chemometric strategy based on design of experiments (DoE) was applied to efficiently optimize the parameters of the electroanalytical techniques employed for PAR detection. The electrode surface, characterized by scanning electron and atomic force microscopy, revealed a significant roughness and defect density, resulting in a larger electrochemically active surface area. The MLG electrodes were evaluated as voltammetric sensors using PAR as the target analyte, since it is relevant in pharmaceutical and environmental analysis. Electrochemical performance was assessed through cyclic voltammetry and square-wave adsorptive stripping voltammetry in various supporting electrolytes. The CO<sub>2</sub> plasma-treated electrode (MLG-t) exhibited notably improved sensitivity toward PAR detection. The optimized sensor exhibited a linear working range of 0.30−8.4 µmol L<sup>−1</sup> and a limit of detection of 0.080 µmol L<sup>−1</sup>. The analysis of the pharmaceutical tablets revealed recovery values in the range of 101.7% to 104.0%. These findings demonstrate that CO<sub>2</sub> plasma treatment, coupled with DoE optimization, represents a valuable strategy for engineering cost-effective and high-performance graphene-based electrochemical sensors.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elan.70078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145521714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
José Guilherme Aquino Rodrigues, Tárcila Mathiasso Nascimento da Silva, Sidnei de Barros Gomes Junior, Antônio Augusto Lopes Marins, Victor Magno Paiva, Isabella Oliveira Britto, Gabriel Fernandes Souza dos Santos, Jairo Pinto de Oliveira, Rafael de Queiroz Ferreira, Natasha Midori Suguihiro, Rogério Valentim Gelamo, Emerson Schwingel Ribeiro, Eliane D’Elia
{"title":"Application of CO2-Plasma Treated Multilayer Graphene as an Electrochemical Sensing Platform for Paracetamol Monitoring","authors":"José Guilherme Aquino Rodrigues, Tárcila Mathiasso Nascimento da Silva, Sidnei de Barros Gomes Junior, Antônio Augusto Lopes Marins, Victor Magno Paiva, Isabella Oliveira Britto, Gabriel Fernandes Souza dos Santos, Jairo Pinto de Oliveira, Rafael de Queiroz Ferreira, Natasha Midori Suguihiro, Rogério Valentim Gelamo, Emerson Schwingel Ribeiro, Eliane D’Elia","doi":"10.1002/elan.70078","DOIUrl":"https://doi.org/10.1002/elan.70078","url":null,"abstract":"<p>Developing advanced materials is crucial for improving electrochemical sensing platforms, particularly by enhancing sensitivity, selectivity, and miniaturization. In this study, we introduce a CO<sub>2</sub> plasma-treated multilayer graphene (MLG) paper as a novel electrode material for the electrochemical detection of paracetamol (PAR). A chemometric strategy based on design of experiments (DoE) was applied to efficiently optimize the parameters of the electroanalytical techniques employed for PAR detection. The electrode surface, characterized by scanning electron and atomic force microscopy, revealed a significant roughness and defect density, resulting in a larger electrochemically active surface area. The MLG electrodes were evaluated as voltammetric sensors using PAR as the target analyte, since it is relevant in pharmaceutical and environmental analysis. Electrochemical performance was assessed through cyclic voltammetry and square-wave adsorptive stripping voltammetry in various supporting electrolytes. The CO<sub>2</sub> plasma-treated electrode (MLG-t) exhibited notably improved sensitivity toward PAR detection. The optimized sensor exhibited a linear working range of 0.30−8.4 µmol L<sup>−1</sup> and a limit of detection of 0.080 µmol L<sup>−1</sup>. The analysis of the pharmaceutical tablets revealed recovery values in the range of 101.7% to 104.0%. These findings demonstrate that CO<sub>2</sub> plasma treatment, coupled with DoE optimization, represents a valuable strategy for engineering cost-effective and high-performance graphene-based electrochemical sensors.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elan.70078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145521849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cover Picture: (Electroanalysis 11/2025)","authors":"","doi":"10.1002/elan.70076","DOIUrl":"https://doi.org/10.1002/elan.70076","url":null,"abstract":"<p>Cover picture provided by Dr. Elena Benito-Peña and Dr. Susana Campuzano. <i>Electroanalysis</i> covers all branches of electroanalytical chemistry, including both fundamental and application papers as well as reviews dealing with analytical voltammetry, potentiometry, new electrochemical sensors and detection schemes, nanoscale electrochemistry, advanced electromaterials, nanobioelectronics, point-of-care diagnostics, wearable sensors, and practical applications.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elan.70076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145371881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cover Picture: (Electroanalysis 11/2025)","authors":"","doi":"10.1002/elan.70076","DOIUrl":"https://doi.org/10.1002/elan.70076","url":null,"abstract":"<p>Cover picture provided by Dr. Elena Benito-Peña and Dr. Susana Campuzano. <i>Electroanalysis</i> covers all branches of electroanalytical chemistry, including both fundamental and application papers as well as reviews dealing with analytical voltammetry, potentiometry, new electrochemical sensors and detection schemes, nanoscale electrochemistry, advanced electromaterials, nanobioelectronics, point-of-care diagnostics, wearable sensors, and practical applications.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elan.70076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145371812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Martina Freisa, Hiu Mun Man, Sandrine Le Guillou, Johann Laubier, Fabienne Le Provost, David Bouville, Isabelle Le Potier, Laurent Thouin, Jean Gamby
{"title":"Label-Free Electrochemical Microfluidic Sensing Platform for the Detection of miR-30b from Milk Samples","authors":"Martina Freisa, Hiu Mun Man, Sandrine Le Guillou, Johann Laubier, Fabienne Le Provost, David Bouville, Isabelle Le Potier, Laurent Thouin, Jean Gamby","doi":"10.1002/elan.70066","DOIUrl":"https://doi.org/10.1002/elan.70066","url":null,"abstract":"<p>Circulating microRNAs (miRNAs) in biological fluids are small non-coding RNAs identified as promising biomarkers. They are particularly abundant in milk, a fluid easily accessible without invasive techniques. However, detection methods are currently performed by specialized personnel in centralized laboratories, which greatly lengthen analysis times. This study addresses some challenges by proposing a portable microfluidic electrochemical platform for the detection of <i>miR-30b</i>, a miRNA overexpressed by a previously developed transgenic mouse model. The sensors integrated into the platform were surface-modified with oligonucleotides. Through dual-mode detection by cyclic voltammetry and electrochemical impedance spectroscopy, they exhibit high specificity and sensitivity with a detection range from 10<sup>−16</sup> to 10<sup>−8</sup> M. Tests on real samples show that the platform is able to distinguish between <i>miR-30b</i> extracted from milk of transgenic mice and that of wild-type mice. These results suggest a strong potential of this sensing platform to carry out early and noninvasive detections. They open the way to precise monitoring of the physiological state of individuals while improving diagnostic options for pathologies in clinical or veterinary settings.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145406513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Martina Freisa, Hiu Mun Man, Sandrine Le Guillou, Johann Laubier, Fabienne Le Provost, David Bouville, Isabelle Le Potier, Laurent Thouin, Jean Gamby
{"title":"Label-Free Electrochemical Microfluidic Sensing Platform for the Detection of miR-30b from Milk Samples","authors":"Martina Freisa, Hiu Mun Man, Sandrine Le Guillou, Johann Laubier, Fabienne Le Provost, David Bouville, Isabelle Le Potier, Laurent Thouin, Jean Gamby","doi":"10.1002/elan.70066","DOIUrl":"https://doi.org/10.1002/elan.70066","url":null,"abstract":"<p>Circulating microRNAs (miRNAs) in biological fluids are small non-coding RNAs identified as promising biomarkers. They are particularly abundant in milk, a fluid easily accessible without invasive techniques. However, detection methods are currently performed by specialized personnel in centralized laboratories, which greatly lengthen analysis times. This study addresses some challenges by proposing a portable microfluidic electrochemical platform for the detection of <i>miR-30b</i>, a miRNA overexpressed by a previously developed transgenic mouse model. The sensors integrated into the platform were surface-modified with oligonucleotides. Through dual-mode detection by cyclic voltammetry and electrochemical impedance spectroscopy, they exhibit high specificity and sensitivity with a detection range from 10<sup>−16</sup> to 10<sup>−8</sup> M. Tests on real samples show that the platform is able to distinguish between <i>miR-30b</i> extracted from milk of transgenic mice and that of wild-type mice. These results suggest a strong potential of this sensing platform to carry out early and noninvasive detections. They open the way to precise monitoring of the physiological state of individuals while improving diagnostic options for pathologies in clinical or veterinary settings.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145406515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}