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Development and validation of a high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry assay for quantification of methoxy-polyethylene glycol propionic acid polymers. 甲氧基聚乙二醇丙酸聚合物的高通量绿色超高性能收敛色谱-串联质谱分析方法的建立与验证。
IF 3.8 2区 化学
Analytical and Bioanalytical Chemistry Pub Date : 2025-06-01 Epub Date: 2025-03-26 DOI: 10.1007/s00216-025-05849-5
Chunpeng Feng, Jiye Tian, Qisheng Fang, Yajie Cheng, Yue Deng, JiaRui Zhang, Shuang Feng, Qingbin Wang, Hecheng Wang, Xuan Zhao, Lei Yin
{"title":"Development and validation of a high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry assay for quantification of methoxy-polyethylene glycol propionic acid polymers.","authors":"Chunpeng Feng, Jiye Tian, Qisheng Fang, Yajie Cheng, Yue Deng, JiaRui Zhang, Shuang Feng, Qingbin Wang, Hecheng Wang, Xuan Zhao, Lei Yin","doi":"10.1007/s00216-025-05849-5","DOIUrl":"10.1007/s00216-025-05849-5","url":null,"abstract":"<p><p>As a synthetic polymer, methoxy-polyethylene glycol propionic acid (M-PEG-PA) is widely used in the biomedicine field. Unraveling the pharmacokinetic behavior of M-PEG<sub>6</sub>-PA in vivo is crucial for evaluating the safety and efficiency of M-PEG-PA-related polymers or drug delivery systems. A high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry (UPC<sup>2</sup>-MS/MS) assay was firstly developed and validated for the determination of M-PEG<sub>6</sub>-PA polymers in a biological matrix. The MRM transition (mass to charge ratio, precursor ions→fragment ions) of m/z 367.2→118.9 was used to quantify M-PEG<sub>6</sub>-PA in this study. The throughput of the assay is high and the total running time for each sample was only 2 min. The linear range of the developed UPC<sup>2</sup>-MS/MS assay for quantification of M-PEG<sub>6</sub>-PA in a biological matrix is 0.05 to 30 μg/mL (R≥0.995). Intra-day and inter-day precisions for the determination of M-PEG<sub>6</sub>-PA by this analytical assay were <6.99%. The absolute recoveries and matrix effects of M-PEG<sub>6</sub>-PA ranged from 79.50 to 92.47% and 68.72 to 81.73%, respectively. The UPC<sup>2</sup>-MS/MS assay was successfully applied to quantify the concentrations of M-PEG<sub>6</sub>-PA polymers in rat plasma samples.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"3091-3102"},"PeriodicalIF":3.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Limitations of the molybdenum blue method for phosphate quantification in the presence of organophosphonates. 有机膦存在时钼蓝法测定磷酸盐的局限性。
IF 3.8 2区 化学
Analytical and Bioanalytical Chemistry Pub Date : 2025-06-01 Epub Date: 2025-03-31 DOI: 10.1007/s00216-025-05850-y
Ruoning Guo, Anna M Röhnelt, Philipp R Martin, Stefan B Haderlein
{"title":"Limitations of the molybdenum blue method for phosphate quantification in the presence of organophosphonates.","authors":"Ruoning Guo, Anna M Röhnelt, Philipp R Martin, Stefan B Haderlein","doi":"10.1007/s00216-025-05850-y","DOIUrl":"10.1007/s00216-025-05850-y","url":null,"abstract":"<p><p>Organophosphonates (OPs) are widely used as chelating agents in domestic and industrial applications. While regarded as hardly biodegradable, OPs can undergo abiotic transformation with phosphate (PO<sub>4</sub><sup>3-</sup>) as a main transformation product. As some OPs are suspected precursors of glyphosate in surface waters, their environmental fate is of current interest. Due to analytical challenges posed by quantification of individual OPs, monitoring PO<sub>4</sub><sup>3-</sup> formation is a widely used proxy to monitor OP transformations. The molybdenum blue (MB) method, employing UV/Vis spectroscopy, is frequently used for PO<sub>4</sub><sup>3-</sup> quantification due to its sensitivity and operational simplicity. However, while interference of certain inorganic ions is well-documented, the effects of OPs on the accuracy of the MB method remain unexplored. This study investigated the effects of six OPs, namely N-(phosphonomethyl)glycine (glyphosate), 1-hydroxyethylidene(1,1-diphosphonic acid) (HEDP), iminodi(methylene phosphonate) (IDMP), aminotris(methylene phosphonate) (ATMP), ethylenediaminetetra(methylene phosphonate) (EDTMP), and diethylenetriaminepenta(methylene phosphonate) (DTPMP). Spectral analysis of pure PO<sub>4</sub><sup>3-</sup> standards using the MB method exhibits two characteristic absorption maxima (λ<sub>max</sub>) at 710 and 880 nm. In the presence of OPs, a new λ<sub>max</sub> appears around 760 nm. This is accompanied by an increase in absorbance values at both 710 and 880 nm, leading to significant over-quantification of PO<sub>4</sub><sup>3-</sup> concentrations. Among the evaluated OPs, DTPMP exhibits the most substantial interference (PO<sub>4</sub><sup>3-</sup> over-quantification by up to 240%), while glyphosate causes minimal interference (≤ 20%). The effects are most pronounced at OPs:PO<sub>4</sub><sup>3-</sup> ratios ≥1. A case study simulating DTPMP transformation confirms PO<sub>4</sub><sup>3-</sup> over-quantification of up to 350%, revealing limitations of the MB method. Therefore, careful data evaluation and complementary analytical techniques for accurate PO<sub>4</sub><sup>3-</sup> measurements are indispensable in OP transformation research.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"3103-3111"},"PeriodicalIF":3.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dual DNA recycling amplification-assisted CRISPR/Cas12a cleavage for dual-channel ratiometric fluorescence biosensing of kanamycin antibiotic. 双DNA循环扩增辅助CRISPR/Cas12a切割用于卡那霉素抗生素双通道比例荧光生物传感。
IF 3.8 2区 化学
Analytical and Bioanalytical Chemistry Pub Date : 2025-06-01 Epub Date: 2025-04-04 DOI: 10.1007/s00216-025-05858-4
Shanshan Lai, Jiahong Guo, Xin Li, Xiaoxiao Yu, Guosong Lai
{"title":"Dual DNA recycling amplification-assisted CRISPR/Cas12a cleavage for dual-channel ratiometric fluorescence biosensing of kanamycin antibiotic.","authors":"Shanshan Lai, Jiahong Guo, Xin Li, Xiaoxiao Yu, Guosong Lai","doi":"10.1007/s00216-025-05858-4","DOIUrl":"10.1007/s00216-025-05858-4","url":null,"abstract":"<p><p>Fluorescence biosensors hold significant importance for testing antibiotic residues which seriously endanger public health. However, how to adopt appropriate strategies to address the false result disadvantage involved in traditional single-channel biosensors is still a great challenge. Meanwhile, too much attention focused on designing signal amplification strategies of biosensors unavoidably decreases their detection efficiency. Herein, we combined the designed dual DNA recycling amplification strategy with CRISPR/Cas12a-mediated dual-channel signal output mode to successfully develop a novel ratiometric fluorescence biosensor for testing kanamycin (Kana) residues in complex sample matrices. The first recycling was formed from an exonuclease-assisted aptamer recognition reaction, which also triggered another cascade DNA recycling to amplify the release of the Cas12a activator. With the non-discrimination cleavage of Cas12a to cause reverse fluorescence changes of copper nanoclusters and an AMAC-labeled signal DNA, the ratiometric signal transduction strategy was constructed. Under optimal conditions, this biosensor could be applied for ultrasensitive testing of Kana antibiotics in a five-order of magnitude wide linear range with a low detection limit of 17.2 fg mL<sup>-1</sup>. Benefiting from the self-correction function of the ratiometric signal transduction mode, it showed promising practicality in lake water and milk samples with the relative error less than 4.9% to the standard ELISA results. Besides CRISPR/Cas12a-based fluorescence output efficiency improvement, this biosensor also excluded the complicated manipulations and expensive instruments required in traditional methods. Therefore, it provides a good choice for expanding the application of fluorescence biosensing technology for practical analysis application.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"3191-3200"},"PeriodicalIF":3.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comprehensive characterization of sulfur compounds in the diesel fraction using methylation/demethylation derived separation and GC × GC-MS/FID analysis. 利用甲基化/去甲基化衍生分离和GC × GC- ms /FID分析对柴油馏分中含硫化合物进行综合表征。
IF 3.8 2区 化学
Analytical and Bioanalytical Chemistry Pub Date : 2025-06-01 Epub Date: 2025-04-07 DOI: 10.1007/s00216-025-05860-w
Yifei Li, Yanfei Su, Zhengyu Chen, Jianxun Wu, Weilai Zhang, Chunming Xu, Quan Shi, Linzhou Zhang
{"title":"Comprehensive characterization of sulfur compounds in the diesel fraction using methylation/demethylation derived separation and GC × GC-MS/FID analysis.","authors":"Yifei Li, Yanfei Su, Zhengyu Chen, Jianxun Wu, Weilai Zhang, Chunming Xu, Quan Shi, Linzhou Zhang","doi":"10.1007/s00216-025-05860-w","DOIUrl":"10.1007/s00216-025-05860-w","url":null,"abstract":"<p><p>Comprehensive characterization of petroleum-derived sulfur compounds is crucial for researching and developing desulfurization catalysts, equipment, and processes. However, the complex composition and low concentration of sulfur compounds in oils make it challenging for molecular-level separation and characterization. In this work, sulfur compounds in the straight-run diesel fraction were selectively separated from the oil by the methylation/demethylation method, effectively yielding high-purity thiophenes and sulfides. Molecular-level compositional and structural characterization of sulfur compounds was accomplished through comprehensive two-dimensional gas chromatography (GC × GC) coupled with time-of-flight mass spectrometry. The separation significantly enhances the detection sensitivity for low-content sulfur compounds, thereby enabling a more comprehensive characterization of their molecular compositions and structures. Sulfur compounds with diverse skeletons and carbon numbers were tentatively characterized, including 1~3 cyclic sulfides, thiophenes, benzothiophenes, dihydrobenzothiophenes, dibenzothiophenes, tetrahydrodibenzothiophenes, phenanthrothiophenes, and benzonaphthothiophenes. Additionally, hundreds of individual sulfur compounds were characterized by mass spectrometry. Quantitative analysis for individual compounds and compound types was conducted using a flame ionization detector.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"3201-3213"},"PeriodicalIF":3.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quality evaluation of metabolite annotation based on comprehensive simulation of MS/MS data from high-resolution mass spectrometry (HRMS) and similarity scoring. 基于高分辨率质谱(HRMS) MS/MS数据综合模拟和相似度评分的代谢物注释质量评价
IF 3.8 2区 化学
Analytical and Bioanalytical Chemistry Pub Date : 2025-06-01 Epub Date: 2025-04-18 DOI: 10.1007/s00216-025-05847-7
Yingjiao Shi, Ji Yang, Qianxu Yang, Yipeng Zhang, Zhongda Zeng
{"title":"Quality evaluation of metabolite annotation based on comprehensive simulation of MS/MS data from high-resolution mass spectrometry (HRMS) and similarity scoring.","authors":"Yingjiao Shi, Ji Yang, Qianxu Yang, Yipeng Zhang, Zhongda Zeng","doi":"10.1007/s00216-025-05847-7","DOIUrl":"10.1007/s00216-025-05847-7","url":null,"abstract":"<p><p>Metabolite annotation is a critical step in discovery metabolomics, but remains a significant challenge. In this study, the accuracy of metabolite annotation was systematically evaluated by leveraging the proposed strategies for simulation of tandem mass spectrometry (MS/MS) data from high-resolution mass spectrometry (HRMS) and then construction of a large-scale virtual database. Furthermore, various similarity scoring methods were comprehensively compared to assess the performance for annotation. First, three key characteristics that are essential for simulating MS/MS spectra to closely resemble experimental data were identified: (i) the number of mass-to-charge ratio (m/z) features, (ii) the differences between neighboring m/z values, and (iii) the intensity distribution of MS/MS features. These factors were employed to generate representative MS/MS spectra for subsequent study. A meticulously designed virtual MS/MS database was constructed to facilitate accurate annotation assessment, which covered over 100,000 metabolites with diverse structural similarities and differences. To evaluate annotation quality, two simulation strategies on the basis of strong and weak data inference were respectively proposed to replicate MS/MS spectra for unknown metabolites. These simulated spectra were then compared with the virtual database, which provided insights into the expected variations in experimental MS/MS data. Furthermore, eight similarity evaluation methods, including entropy similarity (ES) and weighted dot product (W/DP) algorithms, were rigorously evaluated for their effectiveness in metabolite annotation. The results revealed that some methods, such as ES, exhibited strong resistance to interference and broad adaptability across different MS/MS patterns, whereas others selectively yielded reliable outcomes under specific conditions. This study provided a systematic framework for quality evaluation in metabolite annotation and offered strategies to mitigate false-positive identifications. The findings held great significance for advancing metabolomics research and further improving annotation reliability in complex biological samples.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"3061-3077"},"PeriodicalIF":3.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
1H, 15N and 13C backbone resonance assignment of the N-terminal region of Zika virus NS4B protein in detergent micelles. 寨卡病毒 NS4B 蛋白 N 端区域在洗涤剂胶束中的 1H、15N 和 13C 骨架共振分配。
IF 0.8 4区 生物学
Biomolecular NMR Assignments Pub Date : 2025-06-01 Epub Date: 2024-11-07 DOI: 10.1007/s12104-024-10208-z
Yan Li, Ying Ru Loh, Qingxin Li, Dahai Luo, CongBao Kang
{"title":"<sup>1</sup>H, <sup>15</sup>N and <sup>13</sup>C backbone resonance assignment of the N-terminal region of Zika virus NS4B protein in detergent micelles.","authors":"Yan Li, Ying Ru Loh, Qingxin Li, Dahai Luo, CongBao Kang","doi":"10.1007/s12104-024-10208-z","DOIUrl":"10.1007/s12104-024-10208-z","url":null,"abstract":"<p><p>Zika virus has raised global concerns due to its link to microcephaly and Guillain-Barré syndrome in adults. One of viral nonstructural proteins-NS4B, an integral membrane protein, plays crucial roles in viral replication by interacting with both viral and host proteins, rendering it an attractive drug target for antiviral development. We purified the N-terminal region of ZIKV NS4B (NS4B NTD) and reconstituted it into detergent micelles. Here, we report the assignments of the backbone resonances of NS4B NTD in detergent micelles. The available assignment is useful for understanding its structure and ligand binding to provide useful information for developing NS4B inhibitors.</p>","PeriodicalId":492,"journal":{"name":"Biomolecular NMR Assignments","volume":" ","pages":"1-6"},"PeriodicalIF":0.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring Thiazolidinedione-Naphthalene Analogues as Potential Antidiabetic Agents: Design, Synthesis, Molecular Docking and In-vitro Evaluation. 探索噻唑烷二酮萘类似物作为潜在的降糖药:设计、合成、分子对接和体外评价。
IF 1.8 4区 生物学
Cell Biochemistry and Biophysics Pub Date : 2025-06-01 Epub Date: 2024-12-13 DOI: 10.1007/s12013-024-01632-y
Sharfuddin Mohd, Vikas Sharma, Vancha Harish, Rakesh Kumar, Govindaiah Pilli
{"title":"Exploring Thiazolidinedione-Naphthalene Analogues as Potential Antidiabetic Agents: Design, Synthesis, Molecular Docking and In-vitro Evaluation.","authors":"Sharfuddin Mohd, Vikas Sharma, Vancha Harish, Rakesh Kumar, Govindaiah Pilli","doi":"10.1007/s12013-024-01632-y","DOIUrl":"10.1007/s12013-024-01632-y","url":null,"abstract":"<p><p>Thiazolidinedione-naphthalene analogues were synthesized and evaluated for antidiabetic activity as Pancreatic α-Amylase (PAA) and intestinal α-glucosidase (IAG) inhibitors. The activity of the compounds (14a-g,17a-k) is compared with acarbose as the standard drug and all the compounds shows good to moderate antidiabetic activity. In-vitro PAA and IAG inhibition assay is performed for the all compounds, the compounds 17e shows superior PAA and IAG inhibitory activity with respective to standard (IC<sub>50</sub> = 12.455 ± 0.04 μM and 9.145 ± 0. 01 μM). The molecular interaction with PAA and IAG protein was also studied with the help of molecular docking studies using AutoDock software. while SwissADME and Osiris property explorer tools computed in-silico drug likeliness and toxicity properties. The in-silico results confirmed the 17e molecule as a superior drug with high binding affinity and good drug likeness against PAA and IAG, confirming in-vitro results. We also studied antioxidant activity (AOA) of all synthesized compounds and results confined that the compound 14g and 17e has good antioxidant potential IC<sub>50</sub> = 8.04 ± 0.02 μM and 6.36 ± 0.03 μM respectively among all compounds. In conclusion, in-vitro, in-silico antidiabetic and antioxidant studies revealed 17e compound was found to be potential compound.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"2213-2226"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxidative Stress Monitoring Platform: A Longitudinal In vitro Multinuclear (1H/19F) MR Spectroscopic Study. 氧化应激监测平台:纵向体外多核(1H/19F)磁共振光谱研究。
IF 1.8 4区 生物学
Cell Biochemistry and Biophysics Pub Date : 2025-06-01 Epub Date: 2024-12-18 DOI: 10.1007/s12013-024-01640-y
Pravat K Mandal, Yashika Arora, Avantika Samkaria, Joseph C Maroon, Vincenzo Fodale, Yatin Mehta, Yue-Fang Chang
{"title":"Oxidative Stress Monitoring Platform: A Longitudinal In vitro Multinuclear (<sup>1</sup>H/<sup>19</sup>F) MR Spectroscopic Study.","authors":"Pravat K Mandal, Yashika Arora, Avantika Samkaria, Joseph C Maroon, Vincenzo Fodale, Yatin Mehta, Yue-Fang Chang","doi":"10.1007/s12013-024-01640-y","DOIUrl":"10.1007/s12013-024-01640-y","url":null,"abstract":"<p><p>Glutathione (GSH) is a master antioxidant that counters oxidative stress. Clinical studies have confirmed significant depletion of GSH in the hippocampus and the substantia nigra as an early diagnostic biomarker for Alzheimer's disease (AD) and Parkinson disease (PD), respectively. External agents like anesthetics (inhaled and intravenous) have a different impact on GSH. There is significant depletion of the serum GSH peroxidase level after surgery with isoflurane anesthesia that is not found in patients administered intravenous propofol. The objective of this study is to evaluate the GSH level associated with isoflurane in vitro phantom model using non-invasive magnetic resonance (MR) spectroscopy and to detect residual isoflurane in a solution. MRS data was generated utilizing a 3T MR scanner (Prisma, Siemens) equipped with a 64-channel <sup>1</sup>H head coil and dual tune (<sup>19</sup>F/<sup>1</sup>H) head coil. The GSH data acquisition was performed using the MEGA-PRESS pulse sequence using experimental parameters: ON = 4.40 ppm, OFF = 5.00 ppm, TE = 120 ms, TR = 2500 ms, voxel size = 25 × 25 × 25 mm and average = 32. Isoflurane was detected using <sup>19</sup>F MRS studies using <sup>19</sup>F/<sup>1</sup>H head coil. GSH data was processed using KALPANA package and <sup>19</sup>F data was processed using Siemens package. The GSH peak area (without isoflurane) in a phosphate-buffered solution (PBS) solution (control) showed a slow decline over time due to natural oxidation of GSH to dimeric glutathione (GSSG). On the contrary, the GSH peak area in similar model is reduced significantly (p = 0.016) due to isoflurane induced oxidation of GSH to GSSG compared to control. We also report a concise general method for data generation and processing of <sup>1</sup>H MRS data for GSH as well as <sup>19</sup>F monitoring platform using <sup>19</sup>F MR spectroscopy. This is the first report wherein both <sup>1</sup>H and <sup>19</sup>F spectroscopy are applied to generate MRS data along with a unique data processing method. This method is highly sensitive and specifically detects GSH without ambiguity as well as isoflurane due to the unique chemical shift patterns of CF<sub>3</sub> and CHF<sub>2</sub> moieties. This non-invasive MRS approach is developed to monitor GSH-isoflurane interaction leading to oxidative stress and this approach can be extended for other inhaled anesthetics. This methodology using non-invasive <sup>19</sup>F MR spectroscopy needs further development for future clinical studies.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"2281-2288"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Suppression of FcεRI-evoked Degranulation in RBL-2H3 Cells on Gelatin Methacryloyl Hydrogel. 明胶甲基丙烯酰水凝胶对fcε - ri诱导RBL-2H3细胞脱粒的抑制作用。
IF 1.8 4区 生物学
Cell Biochemistry and Biophysics Pub Date : 2025-06-01 Epub Date: 2024-12-28 DOI: 10.1007/s12013-024-01657-3
Haruna Horisaka, Satoru Yokawa, Ruriko Suzuki, Rin Emoto, Rino Maeda, Tadahide Furuno
{"title":"Suppression of FcεRI-evoked Degranulation in RBL-2H3 Cells on Gelatin Methacryloyl Hydrogel.","authors":"Haruna Horisaka, Satoru Yokawa, Ruriko Suzuki, Rin Emoto, Rino Maeda, Tadahide Furuno","doi":"10.1007/s12013-024-01657-3","DOIUrl":"10.1007/s12013-024-01657-3","url":null,"abstract":"<p><p>Cell-extracellular matrix (ECM) interactions play multiple roles in developmental, physiological, and pathological processes. ECM stiffness substantially affects cellular morphology, migration, and function. In this study, we investigated the effect of ECM comprising gelatin methacryloyl (GelMA) on the activation of rat basophilic leukemia (RBL-2H3) cells, a model mast cell line. Maintenance of intracellular Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) elevation and subsequent degranulation, evoked by crosslinking the high-affinity IgE receptors (FcεRI), were significantly suppressed in RBL-2H3 cells on collagen-coated GelMA hydrogel than those on collagen-coated glass dishes and plastic wells. Thapsigargin and phorbol myristate acetate caused sustained [Ca<sup>2+</sup>]<sub>i</sub> increase and degranulation to a similar extent in cells on both GelMA hydrogel and plastic wells/glass dishes. F-actin was clearly accumulated along the periphery of RBL-2H3 cells in plane attached to glass, but not GelMA hydrogel, suggesting that the loose actin cytoskeleton of RBL-2H3 cells on GelMA hydrogel caused suppressive degranulation through unstable FcεRI aggregation.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"2481-2488"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ophiopogonin D Alleviates Sepsis-Induced Acute Lung Injury Through Improving Microvascular Endothelial Barrier Dysfunction via Inhibition of HIF-1α-VEGF Pathway. 麦冬皂苷D通过抑制HIF-1α-VEGF通路改善微血管内皮屏障功能障碍,减轻败血症诱导的急性肺损伤
IF 1.8 4区 生物学
Cell Biochemistry and Biophysics Pub Date : 2025-06-01 Epub Date: 2025-02-01 DOI: 10.1007/s12013-024-01661-7
Yi Fang, Jun Qiu, Yu Xu, Qing Wu, Xing-Chen Huo, Song-Hua Liu
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