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The nonlinear post-buckling behavior of FG cylindrical copper shell reinforced with carbon nanotubes with a polymer core
IF 2.2 3区 工程技术
Archive of Applied Mechanics Pub Date : 2024-12-01 DOI: 10.1007/s00419-024-02710-6
Masoud Ajri
{"title":"The nonlinear post-buckling behavior of FG cylindrical copper shell reinforced with carbon nanotubes with a polymer core","authors":"Masoud Ajri","doi":"10.1007/s00419-024-02710-6","DOIUrl":"10.1007/s00419-024-02710-6","url":null,"abstract":"<div><p>This research analyzes the buckling and post-buckling behavior of composite cylindrical shells with a polymer core and carbon nanotube-reinforced inner and outer copper layers with functional distribution under axial compressive loading. For this purpose, the differential equations governing the nonlinear buckling behavior of these shells were extracted while considering large deformations. Employing the Ritz energy method and considering the Airy function, analytical relations for the buckling load and the critical stress of the structure are extracted. In the following, considering different distributions, the equivalent mechanical properties of the composite structure have been calculated using the law of mixtures. Finally, the effect of different parameters, such as distribution types and volume fraction of nanotubes, on nonlinear buckling loads and post-buckling behavior of these materials is investigated. Results show that increasing the volume fraction of carbon nanotubes reduces the change in the critical length of the cylindrical shell, which happens as a result of increasing Young's modulus and the equivalent stiffness of the shell.</p></div>","PeriodicalId":477,"journal":{"name":"Archive of Applied Mechanics","volume":"95 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study of the log-third-difference method for the computation of even-odd staggering in fission yields
IF 2.6 3区 物理与天体物理
The European Physical Journal A Pub Date : 2024-12-01 DOI: 10.1140/epja/s10050-024-01450-z
Belén Montenegro Viñas, Manuel Caamaño
{"title":"Study of the log-third-difference method for the computation of even-odd staggering in fission yields","authors":"Belén Montenegro Viñas,&nbsp;Manuel Caamaño","doi":"10.1140/epja/s10050-024-01450-z","DOIUrl":"10.1140/epja/s10050-024-01450-z","url":null,"abstract":"<div><p>The even-odd staggering in fission fragments yield distributions is an important observable to address the role of intrinsic excitations in the fission process. However, its computation as a function of the fragment split is an ill-posed problem that is usually solved through certain assumptions. Arguably, the most widely used method for its computation is the log-third-difference method, which assumes an underlying local Gaussian distribution. Experimental data of the even-odd effect show sharp features and peaks that are a challenge for the log-third difference method (or any method for that matter). This paper presents a detailed study of the performance of the log-third-difference method and describes three strategies for improving the precision and accuracy around these sharp features. The best results are obtained with an iterative application of the method and with the use of a multi-Gaussian fit. A hybrid process combining both corrections is also described and applied to real data. In regions with a smooth behaviour, the direct application of the log-third-difference method remains the best option.</p></div>","PeriodicalId":786,"journal":{"name":"The European Physical Journal A","volume":"60 12","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The chondrogenic differentiation of BMSCs in collagen hydrogels and the effect of MMPs among cell-material interactions
Journal of Leather Science and Engineering Pub Date : 2024-12-01 DOI: 10.1186/s42825-024-00176-4
Yajun Tang, Jing Wang, He Qiu, Yang Xu, Zhanhong Liu, Lu Song, Hai Lin, Xingdong Zhang
{"title":"The chondrogenic differentiation of BMSCs in collagen hydrogels and the effect of MMPs among cell-material interactions","authors":"Yajun Tang,&nbsp;Jing Wang,&nbsp;He Qiu,&nbsp;Yang Xu,&nbsp;Zhanhong Liu,&nbsp;Lu Song,&nbsp;Hai Lin,&nbsp;Xingdong Zhang","doi":"10.1186/s42825-024-00176-4","DOIUrl":"10.1186/s42825-024-00176-4","url":null,"abstract":"<div><p>The purpose of this study was to investigate the influence of the advanced structure of collagen on the chondrogenic differentiation of BMSCs encapsulated in collagen hydrogels, with an emphasis on MMPs which might affect the cell-material interactions. Collagen and gelatin-based hydrogels with comparable physicochemical properties but mainly distinctive in molecular structure were prepared and further utilized to load BMSCs to study the chondrogenesis. The detection results of MMPs in hydrogels with and without TIMP at both gene and protein levels suggested that MMPs were involved in cell recognition, adhesion, migration, proliferation and further remodeling of cell microenvironment. The chondrogenic gene detection, histological observation and extracellular matrix analysis indicated that the BMSCs were well differentiated into chondrocytes and maintained the phenotypes in collagen hydrogels (C group) which preserved the native structures, comparing with those results acquired from gelatin hydrogels (G group). Finally, the expression of several integrin subunits was investigated to analyze the connection of these cell membrane surface proteins and microenvironment remodeled by MMPs in collagen and gelatin hydrogels. The conclusion was drawn that the advanced structure of collagen affected the chondrogenesis of BMSCs via the cell-material interactions, among which MMPs were one of the major factors crucial to form appropriate microenvironment to modulate the BMSCs fate.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-024-00176-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study on the Role of EPHB6 in Inhibiting the Malignant Progression of Cervical Cancer C33A Cells by Binding to CBX7. 研究 EPHB6 通过与 CBX7 结合抑制宫颈癌 C33A 细胞恶性进展的作用
IF 1.8 4区 生物学
Cell Biochemistry and Biophysics Pub Date : 2024-12-01 Epub Date: 2024-09-26 DOI: 10.1007/s12013-024-01458-8
Jiancai Wang, Na Zhang
{"title":"Study on the Role of EPHB6 in Inhibiting the Malignant Progression of Cervical Cancer C33A Cells by Binding to CBX7.","authors":"Jiancai Wang, Na Zhang","doi":"10.1007/s12013-024-01458-8","DOIUrl":"10.1007/s12013-024-01458-8","url":null,"abstract":"<p><p>Cervical cancer stands as the most frequently diagnosed malignancy affecting the female reproductive. The erythropoietin-producing hepatocyte (Eph) family tyrosine kinases play important roles in tumorigenesis and cancer aggression. However, the exact role of EPHB6 in cervical cancer remains unknown. The present study investigated the role of EPHB6 in the malignant process of cervical cancer. GEPIA, tnmplot and kmplot database was used to study the expression of EPHB6 in cervical cancer tissues. western blotting was used to detect the expression of EPHB6, CyclinD, CDK4, CDK6, CBX7, MMP2 and MMP9. CCK8 and EDU staining were used to detect cell proliferation. Wound healing and transwell were used to detect cell proliferation and migration. Flow cytometry was used to detect cell cycle level. The linkedomics database was used to predict the correlation of EPHB6 and CBX7 in cervical cancer. Subsequently, HDOCK server was used to predict the combination of EPHB6 and CBX7. Our current results suggested that the expression of EPHB6 is reduced in cervical cancer tissues and cell lines, and the lower the expression, the worse the prognosis. Moreover, overexpression of EPHB6 inhibits cell proliferation, invasion and migration and cycle acceleration of C33A cells. Furthermore, EPHB6 and CBX7 bind to each other in C33A cells, and EPHB6 inhibits cell proliferation, invasion, migration and cell cycle acceleration in cervical cancer by binding to CBX7. EPHB6 expression is reduced in cervical cancer tissues and cells. Its overexpression inhibits proliferation, invasion, migration, and cell cycle acceleration in C33A cells, exhibiting synergy when bound to CBX7.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3703-3713"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Facile biotic/abiotic sandwich detection system for the highly sensitive detection of human serum albumin and glycated albumin. 用于高灵敏度检测人血清白蛋白和糖化白蛋白的简便生物/非生物夹心检测系统。
IF 3.8 2区 化学
Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-07-15 DOI: 10.1007/s00216-024-05403-9
Hirobumi Sunayama, Chehasan Cheubong, Eri Takano, Toshifumi Takeuchi
{"title":"Facile biotic/abiotic sandwich detection system for the highly sensitive detection of human serum albumin and glycated albumin.","authors":"Hirobumi Sunayama, Chehasan Cheubong, Eri Takano, Toshifumi Takeuchi","doi":"10.1007/s00216-024-05403-9","DOIUrl":"10.1007/s00216-024-05403-9","url":null,"abstract":"<p><p>Quantifying glycated albumin (GA) levels in the blood is crucial for diagnosing diabetes because they strongly correlate with blood glucose concentration. In this study, a biotic/abiotic sandwich assay was developed for the facile, rapid, and susceptible detection of human serum albumin (HSA) and GA. The proposed sandwich detection system was assembled using a combination of two synthetic polymer receptors and natural antibodies. Molecularly imprinted polymer nanogels (MIP-NGs) for HSA (HSA-MIP-NGs) were used to mimic capture antibodies, whereas antibodies for HSA or GA were used as primary antibodies and fluorescent signaling MIP-NGs for the Fc domain of IgG (F-Fc-MIP-NGs) were used as a secondary antibody mimic to indicate the binding events. The HSA/anti-HSA/F-Fc-MIP-NGs complex, formed by incubating HSA and anti-HSA antibodies with F-Fc-MIP-NGs, was captured by HSA-MIP-NGs immobilized on the chips for fluorescence measurements. The analysis time was less than 30 min, and the limit of detection was 15 pM. After changing the anti-HSA to anti-GA (monoclonal antibody), the fluorescence response toward GA exceeded that of HSA, indicating successful GA detection using the proposed sandwich detection system. Therefore, the proposed system could change the detection property by changing a primary antibody, indicating that this system can be applied to various target proteins and, especially, be a powerful approach for facile and rapid analysis methods for proteins with structural similarity.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7337-7345"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141615514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Study of the Threshold Stability of the Bilevel Problemof Facility Location and Discriminatory Pricing
IF 0.58
Journal of Applied and Industrial Mathematics Pub Date : 2024-12-01 DOI: 10.1134/S1990478924030165
M. E. Vodyan, A. A. Panin, A. V. Plyasunov
{"title":"A Study of the Threshold Stability of the Bilevel Problem\u0000of Facility Location and Discriminatory Pricing","authors":"M. E. Vodyan,&nbsp;A. A. Panin,&nbsp;A. V. Plyasunov","doi":"10.1134/S1990478924030165","DOIUrl":"10.1134/S1990478924030165","url":null,"abstract":"<p> The problem of threshold stability for a bilevel problem with a median type of facility\u0000location and discriminatory pricing is considered. When solving such a problem, it is necessary to\u0000find the threshold stability radius and a semifeasible solution of the original bilevel problem such\u0000that the leader’s revenue is not less than a predetermined value (threshold) for any deviation of\u0000budgets that does not exceed the threshold stability radius and which preserves its semifeasibility.\u0000Thus, the threshold stability radius determines the limit of disturbances of consumer budgets with\u0000which these conditions are satisfied.\u0000</p><p>Two approximate algorithms for solving the threshold stability problem based on\u0000the heuristic of descent with alternating neighborhoods are developed. These algorithms are based\u0000on finding a good approximate location of facilities as well as on calculating the optimal set of\u0000prices for the found location of facilities. The algorithms differ in the way they compare various\u0000locations of facilities; this ultimately leads to different estimates of threshold stability radius. A\u0000numerical experiment has shown the efficiency of the chosen approach both in terms of the\u0000running time of the algorithms and the quality of the solutions obtained.\u0000</p>","PeriodicalId":607,"journal":{"name":"Journal of Applied and Industrial Mathematics","volume":"18 3","pages":"558 - 574"},"PeriodicalIF":0.58,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of on-site sampling conditions on the representativeness of the sample for the analysis of trace VOCs in raw renewable gases. 现场采样条件对可再生原料气体中痕量挥发性有机化合物分析样本代表性的影响。
IF 3.8 2区 化学
Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-21 DOI: 10.1007/s00216-024-05604-2
A Legendre, S Bsaibes, C Paijens, J Dugay, Y Courtois, L Cuccia, D Ballestas Castro, D Thiebaut, J Vial
{"title":"Influence of on-site sampling conditions on the representativeness of the sample for the analysis of trace VOCs in raw renewable gases.","authors":"A Legendre, S Bsaibes, C Paijens, J Dugay, Y Courtois, L Cuccia, D Ballestas Castro, D Thiebaut, J Vial","doi":"10.1007/s00216-024-05604-2","DOIUrl":"10.1007/s00216-024-05604-2","url":null,"abstract":"<p><p>In the context of the energy transition and in order to fully manage the integration of renewable and/or low-carbon gases into the gas mix, these new renewable gases need to be characterized, including volatile organic compounds (VOCs) at trace levels that may have an impact on different stages of the gas chain event at low concentrations. This study focuses on sampling because it is the first step in any method for analyzing trace VOCs and its careful execution is essential to ensure reliable results even if the on-site conditions can be variable, such as the external temperature. The stabilization time, the effect of external temperature, and the impact of using an intermediate sample cylinder prior to transfer to tubes were hence studied in the laboratory using a standard gas mixture representative of renewable gases. The latter was also studied using a real sample. To perform this study, VOCs were sampled in Tenax® TA tubes and then analyzed by thermal desorption hyphenated to comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry. The results showed that 45 min were required to stabilize the sampling system and that external temperature had little influence, limited to the heaviest compounds. Finally, the use of a cylinder to temporarily collect gas prior to transfer to tubes led to a loss of compounds, particularly when the cylinder was stored before the transfer.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7019-7029"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Visualizing the transdermal delivery of berberine loaded within chitosan microneedles using mass spectrometry imaging. 利用质谱成像技术观察壳聚糖微针中小檗碱的透皮给药。
IF 3.8 2区 化学
Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-14 DOI: 10.1007/s00216-024-05584-3
Xiaoqing Cui, Haoyuan Geng, Huanying Guo, Lei Wang, Zihan Zhu, Yaqi Zhang, Panpan Chen, Xiao Wang, Chenglong Sun
{"title":"Visualizing the transdermal delivery of berberine loaded within chitosan microneedles using mass spectrometry imaging.","authors":"Xiaoqing Cui, Haoyuan Geng, Huanying Guo, Lei Wang, Zihan Zhu, Yaqi Zhang, Panpan Chen, Xiao Wang, Chenglong Sun","doi":"10.1007/s00216-024-05584-3","DOIUrl":"10.1007/s00216-024-05584-3","url":null,"abstract":"<p><p>Berberine (BR), an alkaloid isolated from the Chinese traditional medicine Coptidis rhizoma, exhibits therapeutic effects on several diseases including bacterial infections, diabetes, and hyperlipidemia, but the oral availability is poor. In this work, we prepared the chitosan microneedle array-loaded BR (BR-CS MNAs) to transdermally deliver BR, and the spatial distribution of BR in heterogeneous skin tissues was analyzed and imaged by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). Some endogenous phospholipids with specific spatial distribution were used to differentiate the epidermis and dermis regions of the skin. The results showed that BR was effectively delivered and could permeate to both epidermis and dermis regions of the skin. This demonstrated the feasibility of MALDI-MSI to evaluate the transdermal delivery efficiency of microneedle arrays and suggested BR could be transdermally delivered by CS MNAs.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6869-6877"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Core-shell structures assembled from magnetic covalent organic frameworks as a Ti4+-fixed affinity substrate for the identification of phosphopeptides in colorectal cancer serum. 用磁性共价有机框架组装的核壳结构作为 Ti4+ 固定亲和底物,用于鉴定结直肠癌血清中的磷酸肽。
IF 3.8 2区 化学
Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-28 DOI: 10.1007/s00216-024-05620-2
Jiakai Chen, Xinyue Zhou, Zhenzhen Cao, Bing Wang, Chuan-Fan Ding, Yinghua Yan
{"title":"Core-shell structures assembled from magnetic covalent organic frameworks as a Ti<sup>4+</sup>-fixed affinity substrate for the identification of phosphopeptides in colorectal cancer serum.","authors":"Jiakai Chen, Xinyue Zhou, Zhenzhen Cao, Bing Wang, Chuan-Fan Ding, Yinghua Yan","doi":"10.1007/s00216-024-05620-2","DOIUrl":"10.1007/s00216-024-05620-2","url":null,"abstract":"<p><p>When it comes to the early detection of colorectal cancer (CRC), the study of protein phosphorylation is crucial. This work used magnetic covalent organic framework (COF) materials as substrates. The magCOF@PM-Ti<sup>4+</sup> composites were obtained by constructing the three-dimensional cross-linked structures and chelating them with Ti<sup>4+</sup> in a green and economical way, which was utilized to identify the phosphopeptides. This material possesses magnetic properties that facilitate simplified experimental manipulation, has superior sensitivity (0.2 fmol), excellent selectivity (1000:1), and good reusability (10 cycles), and achieves outstanding recovery rates (103.4 ± 1.2%). In addition, magCOF@PM-Ti<sup>4+</sup> was utilized to identify phosphopeptides in human serum. One hundred phosphopeptides were identified from three normal control serums, whereas 98 phosphopeptides were identified from three CRC serums. The results demonstrated that magCOF@PM-Ti<sup>4+</sup> has great potential in efficiently identifying low-abundance phosphopeptides.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7151-7159"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Illustrate the metabolic regulatory mechanism of Taohong Siwu decoction in ischemic stroke by mass spectrometry imaging. 通过质谱成像,说明桃红四物汤对缺血性中风的代谢调控机制。
IF 3.8 2区 化学
Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-28 DOI: 10.1007/s00216-024-05591-4
Xiaoqun Wang, Xueting Zhang, Jiayu Zhang, Hanxue Yang, Zhuqing Liu, Daiyin Peng, Lan Han
{"title":"Illustrate the metabolic regulatory mechanism of Taohong Siwu decoction in ischemic stroke by mass spectrometry imaging.","authors":"Xiaoqun Wang, Xueting Zhang, Jiayu Zhang, Hanxue Yang, Zhuqing Liu, Daiyin Peng, Lan Han","doi":"10.1007/s00216-024-05591-4","DOIUrl":"10.1007/s00216-024-05591-4","url":null,"abstract":"<p><p>Metabolic dysregulation in the ischemic region has been increasingly recognized as a contributing factor to ischemic stroke pathogenesis. Taohong Siwu decoction (THSWD), a traditional Chinese medicine preparation used to enhance blood circulation, is frequently employed in treating ischemic stroke. However, the metabolic regulatory mechanism underlying the therapeutic effects of THSWD in ischemic stroke remains largely unexplored. In this study, we employed desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to investigate the metabolic changes in the brain tissue of ischemic stroke rat model. Our investigation revealed that 30 metabolites exhibited significant dysregulation in the ischemic brain regions, specifically the cortex and striatum, following ischemic injury. Following the treatment of THSWD, almost all the dysregulated metabolites got different degrees of callback. Further pathway analysis indicated that THSWD might exert its therapeutic effects by restoring energy metabolism, improving neurotransmitter metabolism, recovering polyamine metabolism, and so on. DESI-MSI offers a favorable methodology for investigating the alterations in the spatial distribution and level within the ischemic brain region following treatment with THSWD in ischemic stroke. These findings provide a novel perspective on the underlying mechanisms of the efficacy of THSWD in ischemic stroke treatment.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6931-6944"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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