Analytical Methods最新文献

{"title":"Rapid quantification and classification of five vinca alkaloids used in cancer therapy by flow injection analysis with UV detection and chemometrics.","authors":"Eric Caudron, Cécile Boughanem, Marion Berge, Jehanne Saidi, Antoine Dowek, Laetitia Minh Mai Lê","doi":"10.1039/d5ay00325c","DOIUrl":"https://doi.org/10.1039/d5ay00325c","url":null,"abstract":"<p><p>Vinca alkaloids represent a major class of antineoplastic agents used against cancer. They are prepared in centralized production units by pharmacy technicians. Control of the preparation is indispensable to secure their preparation and avoid any errors, which can have serious consequences for the patient because antineoplastic agents are very toxic. Analytical quality control was proven to be the most efficient control to ensure the right drug at the right dose to the patient. The study focused on vinca alkaloids: vinblastine, vincristine, vindesine, vinflunine, and vinorelbine, in the form of commercially diluted solutions in 0.9% NaCl at therapeutic concentrations. The aim of this study was to develop an analytical methodology for quality control capable of discriminating and quantifying these molecules. The primary objective was to assess the capability of Flow Injection Analysis with UV detection (FIA-UV), combined with chemometrics, for rapid classification and quantification of these alkaloids. A rapid High-Performance Liquid Chromatography with UV-visible detection (HPLC-UV) method was also developed and established as a reference standard. HPLC-UV discrimination was based on retention time, whereas FIA-UV relied on spectral analysis. Therefore, to improve discrimination in FIA-UV, Partial Least Squares Discriminant Analysis (PLS-DA) was incorporated. FIA-UV achieved 100% sensitivity and specificity in discriminating the five alkaloids, demonstrating non-inferiority to HPLC-UV. This method offers a streamlined workflow, reduces iatrogenic risk, and is well suited for antineoplastic agent preparation environments.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel near-infrared fluorescent probe for peroxynitrite imaging in cellular and organ injury.","authors":"Zhifei Zhang, Yang Zhang, Huayu Wang, Chunpo Ge","doi":"10.1039/d5ay00486a","DOIUrl":"https://doi.org/10.1039/d5ay00486a","url":null,"abstract":"<p><p>Peroxynitrite (ONOO^-), a crucial reactive oxygen/nitrogen species, plays a significant role in various physiological and pathological processes. However, its excessive accumulation can lead to severe diseases, necessitating effective detection methods. Herein, we report a novel near-infrared fluorescent probe (DCI-ONOO) for sensitive and selective ONOO^- detection, utilizing dicyanoisophorone as the fluorophore and a diphenylphosphinic group as the recognition moiety. The probe exhibits excellent photophysical properties, including a large Stokes shift (208 nm), good photostability, and a low detection limit (39.8 nM). Upon interaction with ONOO^-, DCI-ONOO demonstrates a significant fluorescence enhancement at 670 nm through an intramolecular charge transfer mechanism. The probe shows remarkable selectivity toward ONOO^- over other reactive oxygen/nitrogen species and maintains stability under various pH conditions. Furthermore, DCI-ONOO displays minimal cytotoxicity and effective membrane permeability, enabling successful imaging of both exogenous and endogenous ONOO^- in living cells. Notably, the probe effectively monitored ONOO^- fluctuations in acetaminophen-induced liver and kidney injury models, revealing previously unreported ONOO^- generation patterns in systemic metabolic organs. These findings demonstrate DCI-ONOO potential as a valuable tool for studying ONOO^--related biological processes and drug-induced organ damage.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Masterful ecofriendly maintainable spectrophotometric techniques for considering indacaterol acetate and mometasone furoate: full evaluation and comparison of their greenness, whiteness, and blueness profiles.","authors":"Selvia Maged Adly, Hany Hunter Monir, Safa'a Mohamed Riad, Mohamed Ahmed Elsayed","doi":"10.1039/d5ay00159e","DOIUrl":"https://doi.org/10.1039/d5ay00159e","url":null,"abstract":"<p><p>People of all ages may suffer from asthma. According to recent research, those who have poorly controlled asthma may be more susceptible to hospitalization and severe symptoms from any respiratory infection, particularly severe ones like the COVID-19 and human metapneumovirus (HMPV) viruses, which could result in the death of these critically ill people. Therefore, a study of the mixture of indacaterol acetate and mometasone furoate which is used in the new remarkable anti-asthmatic Breezhaler will produce substantial gains for the scientific field. A binary blend of (INDA) indacaterol acetate (1.00-30.00) μg mL^-1, and (MOM) mometasone furoate (1.00-40.00) μg mL^-1 was estimated over these ranges using six sensitive, neoteric, rapid, frugal, universal, and explicit spectrophotometric methods using the most satisfactory green solvent: ethanol. These methods are the dual wavelength method, ratio subtraction method, factorized absorbance difference method, advanced absorbance subtraction method, derivative ratio method, and derivative amplitude factor method. These procedures have been sensitively and effectively utilized for the analysis of INDA and MOM in commercial Breezhaler capsules with multiple ratios for the first time with low LOD (0.186 and 0.172) and LOQ values (0.565 and 0.520) for INDA and MOM, respectively, superior to those in other published spectrophotometric work, and they were validated in accordance with ICH specifications. Additionally, they delivered 98-102% recovery percentages and showed RSD% of less than 2 for the studied drugs, confirming their specificity. Ecological aspects of the developed work were evaluated and gave satisfactory results using several evaluation tools [such as NEMI, Eco-Scale (=93), GAPI, AGREE (=76)] for the greenness aspect, RGB (=96.3) for the whiteness aspect, and BAGI (=90) for the blueness aspect. Additionally, an environmental assessment and statistical comparisons were made using Student's <i>t</i>-test and the <i>F</i>-test with the published HPLC method and those methods currently in use.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-responsive ratiometric fluorescent probe for the detection of hypochlorite and hydrazine in environmental samples, live cells, and plant tissues.","authors":"Dipanjan Banik, Shilpita Banerjee, Satyajit Halder, Rajdeep Ganguly, Anirban Karak, Pintu Ghosh, Kuladip Jana, Ajit Kumar Mahapatra","doi":"10.1039/d5ay00153f","DOIUrl":"https://doi.org/10.1039/d5ay00153f","url":null,"abstract":"<p><p>Hypochlorite (ClO^-), a potent oxidizer and disinfectant, and hydrazine, a powerful reducing agent, are widely used in daily life and various industries. However, their extensive use comes with significant risks, as they are highly toxic to both the environment and human health. They have been associated with various health issues and even linked to cancer. Therefore, the simultaneous detection of hypochlorite and hydrazine is crucial for assessing their impact and monitoring the onset and progression of related diseases. A phenanthroimidazole-indandione based colorimetric and ratiometric fluorescent probe PIID was designed and synthesized for dual channel detection of hypochlorite and hydrazine in environmental and biological samples. Probe PIID, which showed a strong yellow-orange emission at 640 nm with a massive Stokes shift of 220 nm, exhibited excellent fluorescence change from yellow-orange to green (526 nm) in the presence of ClO^- and from yellow-orange to blue (424 nm) in the presence of hydrazine in an aqueous-THF solvent system. A strong ICT effect, which was acting in probe PIID, gets weakened through ClO^- - mediated cleavage of the CC bridge bond to produce aldehyde PIB with a blue shift of 114 nm and hydrazine-induced hydrazinolysis of the indanedione moiety to form hydrazone compound PIBH with a blue shift of 216 nm and that was also confirmed by DFT studies. Not only that, the probe exhibits excellent selectivity over other ROS (reactive oxygen species) and amines with a very fast response time of 40 seconds for hypochlorite and 90 seconds for hydrazine, and high sensitivity was observed with detection limits of 32.75 nM for hypochlorite and 92 nM for hydrazine. Moreover, PIID was employed to monitor both the analytes successfully in environmental water samples and in a solid-state TLC strip study. Hypochlorite was monitored in commercial disinfectants, and by exogenous bioimaging in human breast cancer cells (MDA-MB 231) and endogenous bioimaging in RAW 264.7 macrophage cells with very low cytotoxicity and good cell viability. Meanwhile, hydrazine was tracked in soil samples, and confocal imaging was performed on onion tissue.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensing technology empowering food safety: research progress of SERS-assisted multimodal biosensing toward food hazard factors.","authors":"Jiaqian Liu, Xiaowei Huang, Xinai Zhang, Yuerong Feng, Zhecong Yuan, Shujie Gao, Zhihua Li, Hany S El-Mesery, Jiyong Shi, Xiaobo Zou","doi":"10.1039/d5ay00292c","DOIUrl":"https://doi.org/10.1039/d5ay00292c","url":null,"abstract":"<p><p>Food is the main source of human energy and nutrition, but once it is contaminated with hazardous factors, such as biotoxins, pesticide residues, <i>etc.</i>, it will seriously damage health. This paper reviews the research progress of biosensors based on surface-enhanced Raman scattering (SERS) in the detection of food hazard factors. First, the basic principle, substrate and assay mode of SERS technology, as well as related design and sensing strategy mechanisms, are introduced. Then, the design idea of multimodal biosensors combining SERS with microfluidic, fluorescence, colorimetric, electrochemical (EC), molecular imprinting and other technologies is expounded to improve the analysis accuracy and specificity. Then the application results of multimodal biosensors based on SERS sensing toward food hazard factors are discussed, and the necessity of its development is illustrated. Finally, the future development direction of this field is prospected, which provides a reference for promoting the research and application of multimodal biosensors based on SERS.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective extraction of trace cytokinins in plants based on a carboxyl functionalized microporous organic network.","authors":"Li Chen, Tao Yu, Le Huang, Qiaomei Lu, Wenmin Zhang, Lan Zhang","doi":"10.1039/d5ay00123d","DOIUrl":"https://doi.org/10.1039/d5ay00123d","url":null,"abstract":"<p><p>Cytokinin (CK) is a type of hormone that exists widely in plants and plays a significant role in promoting cell proliferation and division. Developing a rapid and sensitive method for the detection of trace CKs remains challenging. Microporous organic networks (MONs) are novel materials widely used in sample preparation owing to their excellent extraction performance. Therefore, a dispersive solid phase extraction method based on carboxylated microporous organic networks (MON-2COOH), combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the detection of four CKs was developed. Upon introducing a carboxyl group into pure MON, the MON-2COOH material had excellent stability, high porosity, and a good dispersion effect, and provided more interaction sites to achieve good adsorption of target compounds. Under the best conditions, the established dSPE-UPLC-MS/MS method had the advantages of low adsorbent dosage (2 mg), low detection limit (1.0-10.0 pg mL^-1) and good reproducibility (RSD ≤ 6.54%, <i>n</i> = 5). Synergistic extraction mechanisms involving π-π, hydrogen bonding and hydrophobic interactions were elucidated using both density functional theory calculations and experimental data. This work also confirmed the feasibility of functionalized MONs as satisfactory adsorbents for the enrichment of small organic compounds.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of phenolic lignin dimer models for the quantification of monolignols in biomass and in its derived organosolv lignins <i>via</i> thioacidolysis and GC-MS analysis.","authors":"T Tran Ho, Olivia-Stella Salm, Tiit Lukk, Maria Kulp","doi":"10.1039/d5ay00073d","DOIUrl":"https://doi.org/10.1039/d5ay00073d","url":null,"abstract":"<p><p>A thorough understanding of lignin's fundamental chemistry in lignocellulosic materials is essential for maximizing the efficiency of biorefineries. Thioacidolysis, followed by gas chromatography-mass spectrometry (GC-MS), has emerged as a reliable method for quantifying uncondensed monolignols, which are linked by labile aryl ether bonds within lignin network. However, the lack of commercially available pure thioethylated lignin monomers for GC analysis poses a challenge. This necessitates a multi-step synthesis process, which may not be feasible for all laboratories. We propose a novel approach that utilizes readily available phenolic lignin model dimers to establish a calibration curve for thioacidolysis quantification. These dimers, guaiacylglycerol-β-guaiacyl ether (GGE) and syringylglycerol-β-guaiacyl ether (SGE), upon thioacidolysis, yield thioethylated non-condensed guaiacyl (G) and syringyl (S) monomers. The GC-MS responses of these monomers are compared to those of bisphenol E, an internal standard (IS) to generate the calibration curve. This methodology exhibits excellent performance characteristics and was successfully employed to determine the thioethylated monomer contents and calculate of S/G ratios in three representative biomasses: aspen, barley straw, pine, and their organosolv lignin extracts.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemiluminescence immunosensor for the sensitive detection of the pancreatic neuroendocrine tumor biomarker chromogranin B based on graphdiyne quantum dots.","authors":"Yunfan Zhou, Min Guo, Yuanliang Li, Huangying Tan, Xu Chen, Wensheng Yang","doi":"10.1039/d5ay00060b","DOIUrl":"https://doi.org/10.1039/d5ay00060b","url":null,"abstract":"<p><p>Neuroendocrine tumor (NET) is a rare and heterogeneous tumor with an increasing incidence, which is often misdiagnosed and frequently presents with distant metastases at the time of diagnosis. Chromogranin B (CgB) is not influenced by other diseases or drug factors, such as proton pump inhibitors (PPIs), and is therefore expected to serve as a complementary biomarker to improve the early diagnosis of NET. However, the detection sensitivity of CgB urgently requires improvement. In this study, a highly sensitive ECL immunosensor was designed for the detection of CgB. Graphdiyne quantum dots (GDY QDs), synthesized <i>via</i> the hydrothermal treatment of graphdiyne (GDY) nanosheets, exhibited good biocompatibility and a high electrochemiluminescence (ECL) quantum yield (<i>Φ</i>_ECL = 218.5%) when using K₂S₂O₈ as a co-reactant. With remarkable conductivity and a large surface area, Au NPs effectively loaded a high amount of GDY QDs, thereby enhancing the ECL response. Therefore, the developed ECL immunosensor showed exceptional analytical performance for CgB detection, demonstrating excellent linearity over a range of 10 fg mL^-1 to 100 ng mL^-1, with a limit of detection as low as 1.84 fg mL^-1 (S/N = 3). Moreover, this immunosensor could detect CgB levels in clinical serum and functioned synergistically with enzyme-linked immunosorbent assay (ELISA). This work expands the application of GDY QDs in ECL biosensors while also providing a highly sensitive detection method for CgB.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering gunshot residue flow and deposition mechanisms using novel visualization methods, real-time atmospheric particle sampling, and spectrochemical techniques.","authors":"Thomas D Ledergerber, Matthew Staymates, Kourtney A Dalzell, Luis E Arroyo, Roger Jefferys, Tatiana Trejos","doi":"10.1039/d4ay02283a","DOIUrl":"https://doi.org/10.1039/d4ay02283a","url":null,"abstract":"<p><p>Gunshot residue (GSR) consists of inorganic and organic components released during firearm discharge. Understanding the generation, transport, and settlement of these residues is essential to assess exposure risks and answer questions of forensic interest. Since GSR is prone to depositing in the vicinity of a firing event, its presence on a person of interest is meaningful to evaluate hypotheses about who discharged a firearm or if GSR was acquired by alternative means such as indirect transfer, being a bystander, or passing through the area shortly thereafter. However, the complexity of GSR production and variable dispersion makes its interpretation challenging. This study employs a novel multi-sensor approach to enhance the current understanding of GSR deposition, transference, and persistence. First, a particle counting/sizing system and inexpensive custom-made atmospheric samplers measure the population of airborne particles before, during, and after the firearm discharge. Second, high-speed videography and laser sheet scattering reveals visual and qualitative information about the flow of GSR under various experimental conditions. Finally, SEM-EDS and LC-MS/MS permit the confirmation of the elemental and chemical makeup of residues. This study estimates (a) how IGSR/OGSR are produced during a firing event using various firearms and ammunition, (b) how long it takes to settle on surfaces located at various distances from the firing location, and (c) direct and indirect deposition in indoor, semi-enclosed, and outdoor environments. The combination of these analytical tools provides breakthrough knowledge in forensics and other disciplines where airborne exposure is central, such as environmental sampling and indoor air quality.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an iron-metal organic Framework-based dispersive solid phase extraction coupled with flame atomic absorption spectrophotometry for the accurate and sensitive determination of copper in lion's claw tea samples.","authors":"Zeynep Teki N Semerci, K Übra Karakebap, Devrim Nur Karaman, Eylül Yilmaz, Gamze Dalgiç Bozyi Ği T, Elif Öztürk Er, Sezgin Bakirdere","doi":"10.1039/d5ay00272a","DOIUrl":"https://doi.org/10.1039/d5ay00272a","url":null,"abstract":"<p><p>An iron-based metal organic framework (Fe-MOF) constructed using terephthalic acid as an organic linker was synthesized and characterized for its implementation in a dispersive solid-phase extraction (dSPE) procedure. This strategy was combined with a flame atomic absorption spectrophotometry (FAAS) system and employed for the preconcentration and trace level detection of copper in lion's claw tea samples. Under optimal conditions, the Fe-MOF based dSPE-FAAS method achieved a limit of detection (LOD) of 2.0 μg L^-1 and a limit of quantification (LOQ) of 6.7 μg L^-1. The determination coefficient (<i>R</i>²) was found to be 0.9984 for the concentration range of 5.0-100 μg L^-1, indicating superior linearity for the developed Fe-MOF-dSPE-FAAS method. The sensitivity was enhanced by 88.2 folds. Moreover, acceptable recovery percentages at five different spiked levels within the concentration range of 7.5-75 μg L^-1 for the lion's claw tea sample matrix were achieved through the application of a matrix matching strategy.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}