{"title":"Chitosan as a fluorescent probe for the detection of the AIE-active food colorant quinoline yellow.","authors":"Yuan Gu, Jianwei Wu, Bingyong Lin, Yueliang Wang, Yuanyuan Yao, Lifen Chen, Jianguo Xu, Longhua Guo","doi":"10.1039/d4ay02087a","DOIUrl":"https://doi.org/10.1039/d4ay02087a","url":null,"abstract":"<p><p>The greenish-yellow synthetic dye quinoline yellow (Qy) is widely used in the food and pharmaceutical industries. However, this dye may lead to health and environmental problems. Therefore, investigating how Qy interacts with biological macromolecules is of great interest. In this work, Qy was found to be a novel AIEgen having strong solid-state emission and water-solubility. Adding tetrahydrofuran to an aqueous solution of Qy induced Qy to form nanoaggregates, which increased its fluorescence intensity. Moreover, we found that Qy was able to interact with typical biological macromolecules, such as chitosan, BSA, and DNA, and quench these biomolecules' intrinsic fluorescence. Therefore, chitosan was chosen as a probe for Qy detection. The results showed that chitosan could detect Qy in the presence of interfering ions, other dyes, and sucrose, as well as in an acidic environment. Finally, chitosan was used to determine the quantity of Qy in orange juice and wine. This is the first report of the identification of a food colorant as an AIEgen, and this AIE activity has been wisely harnessed to visualize molecular interactions between Qy and biological macromolecules, as well as to detect Qy in beverages.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bo Yu, Qiuping Li, Wei Zhang, Xiaoyan Xiang, Danzeng Quda, Gasong Zhuoga, Qiong Huang, Jie Cheng, Jinhong Guo, Zhijuan Liu, Li Shi
{"title":"Development and clinical validation of photochemical biosensors for monitoring hemoglobin, blood lipids and uric acid in plateau areas.","authors":"Bo Yu, Qiuping Li, Wei Zhang, Xiaoyan Xiang, Danzeng Quda, Gasong Zhuoga, Qiong Huang, Jie Cheng, Jinhong Guo, Zhijuan Liu, Li Shi","doi":"10.1039/d4ay01737d","DOIUrl":"https://doi.org/10.1039/d4ay01737d","url":null,"abstract":"<p><p>High-altitude regions are prone to plateau erythrocytosis due to unique geo-climatic conditions such as low oxygen, high altitude, and low temperatures, with significantly higher incidences of hyperlipidemia and hyperuricemia compared to lowland areas. However, the extreme environmental conditions at high altitudes and the elevated hematocrit levels in blood samples from these populations present significant challenges to the applicability of existing point-of-care testing (POCT) devices. This study describes the development, early clinical validation, and potential clinical impact of three portable, paper-based photochemical biosensing platforms specifically designed for use at high altitudes to monitor hemoglobin, lipids, and uric acid in blood samples. To systematically evaluate the performance of these sensors, 154 fresh venous blood samples from three distinct altitudes were tested and compared with a reference measurement system, alongside an extensive evaluation for the first time of the suitability of various commercially available POCT devices, including these platforms, for use in high-altitude environments. The results demonstrated that the accuracy and concordance between the developed platforms and the reference system were consistently above 95%. Additionally, concordance remained above 95% when the same samples were tested at varying altitudes (4500 m to 3650 m and 4800 m to 3650 m). All of this indicates that the sensing platform provides high accuracy and reliability for testing in high-altitude environments. It holds promise for broader application in the screening and management of chronic diseases in high-altitude and other extreme environments, and for advancing the use of bedside testing technology in resource-limited settings.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bruno Vasconcellos Lopes, Guilherme Kurz Maron, Mateus Gallucci Masteghin, Raphael Dorneles Caldeira Balboni, S Ravi P Silva, Neftali Lenin Villarreal Carreno
{"title":"Direct-detection of glyphosate in drinking water <i>via</i> a scalable and low-cost laser-induced graphene sensor.","authors":"Bruno Vasconcellos Lopes, Guilherme Kurz Maron, Mateus Gallucci Masteghin, Raphael Dorneles Caldeira Balboni, S Ravi P Silva, Neftali Lenin Villarreal Carreno","doi":"10.1039/d4ay01549e","DOIUrl":"https://doi.org/10.1039/d4ay01549e","url":null,"abstract":"<p><p>The use of pesticides has significantly increased and proliferated following the technological advancements established by the green revolution, aimed at boosting agricultural productivity. The extensive use of man-made chemicals as fertilizer and pesticides has consequently led to large-scale application, which has led to a number of environmental and human health problems. This study has helped to develop a laser-induced graphene (LIG) sensor for the detection of the most widely used herbicide in the world, glyphosate. The electrochemical sensor developed is based on a three-dimensional porous and fibrous structure with nanosheets, making it suitable for scalable manufacture. The study was conducted utilising a linear voltammetry technique and demonstrates the potential to identify glyphosate with good sensitivity. The sensor exhibited detection and quantification limits of 2.7 μmol L<sup>-1</sup> and 9.0 μmol L<sup>-1</sup>, respectively, and showed good selectivity without significant interference from other elements. The sensor presents advantages suitable for scalable production, with case studies in screening of glyphosate-contaminated samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The role of DNA nanotechnology in medical sensing.","authors":"Darell Lien","doi":"10.1039/d4ay01803f","DOIUrl":"https://doi.org/10.1039/d4ay01803f","url":null,"abstract":"<p><p>This paper explores how DNA nanotechnology enhances biosensors in medicine and pharmacology by taking advantage of the unique characteristics of DNA and the unique advantages of DNA origami technology. DNA origami allows the establishment of complex nanoobjects with precise size and complete molecular writability as well as the possibility of seamless integration and biocompatibility with biological systems. Utilizing this, the chemical denaturation of DNA chains allows for the combination of various functions, including organic fluorescence groups and photoreaction elements, <i>etc.</i> This has allowed DNA origami to become a transformative tool in biotechnology and other fields because of its versatility, use in innovative applications improving the design and function of biosensors, and potential to provide greater possibilities for early disease diagnosis and personalized medicine.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A fluorescent probe based on pyrazoline with significant Stokes shifts for the detection of Cu<sup>2+</sup> ions and its applications.","authors":"Yajing Shang, Xinghu Wu, Haoting Luo","doi":"10.1039/d4ay01772b","DOIUrl":"https://doi.org/10.1039/d4ay01772b","url":null,"abstract":"<p><p>A simple pyrazoline derivative BBD has been synthesized for detecting Cu<sup>2+</sup> in EtOH/HEPES (v/v = 1 : 1, pH = 7). The probe has high selectivity for Cu<sup>2+</sup> by quenching the fluorescence intensity and was sensitive to pH. When excited at 398 nm, fluorescence is emitted at 520 nm with a Stokes shift of 122 nm, which is larger than that of other reported pyrazoline skeleton probes. The detection limit (LOD) of the probe for Cu<sup>2+</sup> is 0.862 μM which is lower than the value of the WHO limit (31.5 μM) in water. The 2 : 1 binding mode and probable recognition mechanism of the probe with Cu<sup>2+</sup> were confirmed by Job's plot, IR, ESI-MS, and TD-DFT analysis. Moreover, the probe has been successfully applied to actual water samples as well as cell and zebrafish imaging.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Automatic magnetic solid phase extraction for rapid and high-throughput determination of neonicotinoid insecticides and their metabolites in serum, breast milk and urine samples.","authors":"Kaiqin Huang, Jing Yi, Guocheng Liu, Yangyang Liu, Kaixin Jiang, Zhuowen Li, Yanji Qu, Meiqing Lin, Shengtao Ma","doi":"10.1039/d4ay01244e","DOIUrl":"https://doi.org/10.1039/d4ay01244e","url":null,"abstract":"<p><p>In this study, an automatic magnetic solid phase extraction method was developed to determine six parent neonicotinoids (NEOs) and three of their metabolites in breast milk, serum and urine samples. As key extraction parameters, the sorbent amount, washing solvent and elution solvent were optimized to 4 mg of HLB packing magnetic sorbent, pure water and acetonitrile, respectively. Recoveries of the analytes ranged between 81% and 121% for bovine milk samples, 64% and 122% for fetal bovine serum samples and 81% and 109% for pooled urine samples, with RSDs of <20%. The intra-day and inter-day variations were 2.7-14.9% and 1.2-13.4%, respectively, for all analytes in the three matrices. The limit of quantitation ranged from 0.002-0.05 ng mL<sup>-1</sup>, 0.002-0.06 ng mL<sup>-1</sup> and 0.012-0.348 ng mL<sup>-1</sup> for the target compounds in bovine milk, fetal bovine serum and pooled urine samples, respectively. The validated method was successfully applied for biomonitoring of NEOs in real samples. Notably, the developed method required only 200 μL of sample and 1.4 mL of organic solvent to prepare a batch of 32 samples in less than 30 min, making it suitable for large-scale epidemiological biomonitoring of human exposure to NEOs or equivalent agrochemicals.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zeinab Feyzollahi, Mahdiye Hassanpoor, Afsaneh Orouji, Mohammad Reza Hormozi-Nezhad
{"title":"Morphology-dependent nanoplasmonic assay: a powerful signaling platform for multiplexed total antioxidant capacity analysis.","authors":"Zeinab Feyzollahi, Mahdiye Hassanpoor, Afsaneh Orouji, Mohammad Reza Hormozi-Nezhad","doi":"10.1039/d4ay01990c","DOIUrl":"https://doi.org/10.1039/d4ay01990c","url":null,"abstract":"<p><p>Assessing the total antioxidant capacity (TAC) in biological samples, such as saliva, is essential for health monitoring and disease prevention. TAC plays a critical role in protecting cells from damage caused by free radicals and oxidative stress, which are associated with various conditions, including cancer, cardiovascular diseases, and aging. Key antioxidants, including ascorbic acid (AA), cysteine (CYS), glutathione (GSH), and uric acid (UA), significantly contribute to this protective effect, with salivary levels of these antioxidants reflecting their concentrations in the bloodstream. Therefore, there is a strong demand for a robust, non-toxic colorimetric sensor that can effectively monitor these antioxidants using an innovative approach. This study introduces a multi-colorimetric probe capable of generating high-resolution, naked-eye-detectable color readouts for evaluating salivary TAC. The probe utilizes the morphology-dependent properties of plasmonic nanostructures as recently developed colorimetric sensors, enabling precise and efficient analysis of salivary antioxidants. The assessment of antioxidants was conducted using the probe in combination with pattern recognition analysis for accurate identification and regression analysis for quantification. The probe exhibited linear responses to pure antioxidants and TAC over a broad concentration range: 3.1-60.0, 2.6-60.0, 1.2-20.0, 0.8-20.0, and 0.7-14.0 μmol L<sup>-1</sup>, with detection limits of 1.1, 0.9, 0.4, 0.3, and 0.2 μmol L<sup>-1</sup> for AA, CYS, GSH, UA, and TAC-mixture, respectively. Moreover, performance metrics highlight the robustness and efficacy of the probe in detecting and quantifying antioxidant levels in saliva samples. The efficacy of the developed multi-colorimetric probe was rigorously validated through the analysis of real saliva samples for on-site TAC monitoring. This rapid, cost-effective, user-friendly, non-toxic, and non-invasive method allows for a comprehensive analysis of both individual and total antioxidants, making it highly applicable for health monitoring and disease prevention. Additionally, the probe generates unique response profiles based on varying ratios of endogenous antioxidants, enabling precise TAC quantification in saliva-an essential factor for clinical diagnostics.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A metal organic framework, UiO-66-NH<sub>2</sub>, based on a molybdenum Schiff base complex for the efficient electrochemical determination of diphenoxylate.","authors":"Samira Saeednia, Masoud Rezaeinasab, Parvaneh Iranmanesh, Sobhan Abbasi Razgaleh","doi":"10.1039/d4ay01957a","DOIUrl":"https://doi.org/10.1039/d4ay01957a","url":null,"abstract":"<p><p>Diphenoxylate, an agonist and opioid agent, is applied to enhance the activity of the circular muscle of the intestine. In this work, we prepared a novel electrochemical sensor for the determination of diphenoxylate based on a modified UiO-66-NH<sub>2</sub> metal-organic framework (UiO-66-NH<sub>2</sub> MOF) using a molybdenum Schiff base complex and NiS nanoparticles (NiSnp) in a carbon paste electrode (CPE). The UiO-66-NH<sub>2</sub> MOF and UiO-66@Schiff-base-Mo were studied through advanced analysis techniques, such as X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDAX) and transmission electron microscopy (TEM), to reveal the inherent characteristics of the material. The results also showed that the morphology and structure of the UiO-66-NH<sub>2</sub> MOF were maintained after surface modification. The electrochemical properties of the proposed modified electrode (NiSnp/MOF@Mo/CPE) were characterized using cyclic voltammetry (CV). Also, a differential pulse voltammetry (DPV) method was employed for diphenoxylate determination with NiSnp/MOF@Mo/CPE. A linear range was achieved from 1.0 to 55.0 μM and 65.0 to 125.0 μM, and the detection limit was found to be 0.34 μM. The capability of the fabricated sensors based on NiSnp/MOF@Mo/CPE was investigated through diphenoxylate determination in real samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Na Li, Yan Zhang, Dongyu Zhang, Xiaoli Zhang, Ning Wei, Xiuhui Liu
{"title":"Au nanoparticle-modified hollow carbon spheres as an advanced electrochemical sensing platform for effective detection of homocysteine in human serum.","authors":"Na Li, Yan Zhang, Dongyu Zhang, Xiaoli Zhang, Ning Wei, Xiuhui Liu","doi":"10.1039/d4ay01766h","DOIUrl":"https://doi.org/10.1039/d4ay01766h","url":null,"abstract":"<p><p>It is widely known that homocysteine (Hcy) is associated with the pathogenesis of many clinical diseases, and its quantitative detection can help early treatment of related diseases. The selection of materials with excellent properties is important for the construction of electrochemical sensing platforms and quantitative detection of Hcy. In this study, two nanocomposites, Au nanoparticles/hollow carbon spheres (AuNPs@HCS) and Au nanoparticles/solid carbon spheres (AuNPs@SCS), were prepared first. Then their morphology, structure and electrochemical behavior were investigated. And the comparison found that the HCSs have a large electroactive surface area and outstanding conductivity. Thanks to the catalytic properties of AuNPs and the synergistic effect of HCSs, AuNPs@HCS/GCE showed excellent electrochemical sensing performance for a wide range of Hcy concentrations with a detection limit down to 4.44 nM (S/N = 3). Moreover, the electrochemical mechanism of Hcy on Au@HCS/GCE is discussed in detail. Finally, in the actual serum sample assay, the sensor is used to detect Hcy directly and the recovery rate is satisfactory. This work may provide a valid method for the quantitative determination of Hcy in the future.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A simple and stable dual-emission fluorescent system based on R6G/Eu-MOF for the rapid and sensitive determination of orientin.","authors":"Huifeng Xu, Weihua Huang, Rui Pan, Ronglin Chen, Lili Wang, Yun Yu, Xihai Li, Xi Zhu","doi":"10.1039/d4ay01610f","DOIUrl":"https://doi.org/10.1039/d4ay01610f","url":null,"abstract":"<p><p>A stable dual-emission fluorescent system, R6G/Eu-MOF, was obtained by simple mixing Eu-MOF with fluorescent rhodamine 6G (R6G). This system not only achieved robust dual luminescence performance at 430 nm (Eu-MOF) and 555 nm (R6G) under single-wavelength excitation, but also endowed it with significantly improved fluorescence stability. Orientin (OT), a bioactive traditional Chinese medicine (TCM) component, was found to efficiently quench the dual fluorescent emissions <i>via</i> the synergistic effect of dynamic quenching, photo-induced electron transfer and molecular interactions. Therefore, a rapid fluorescent assay for OT was developed based on the variations in fluorescence signals at 430 nm and 555 nm, as well as the alterations in the ratio between these two signals. This method can achieve highly sensitive determination of OT in the linear range of 1.1-220 μM, with a LOD of 0.26 μM at 430 nm and 0.16 μM at 555 nm (3<i>σ</i>/<i>N</i>). The R6G/Eu-MOF hybrid exhibits significantly lower detection limits and a broader linear range for OT than either R6G or Eu-MOF alone. This work presents a simple strategy to improve the water stability of Eu-MOF and also provides a rapid analysis of small molecule drugs using a fluorescence approach based on a dual-emission MOF hybrid system.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}