Analytical Methods最新文献

筛选
英文 中文
Near-infrared spectroscopy: application in ensuring food quality and safety 近红外光谱:在保证食品质量安全中的应用
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-23 DOI: 10.1039/D4AY02039A
Yuxia Xu, Tianyu Kong, Yinfei Ma, Yan Zhao, Le Chu and Mingzhu Zheng
{"title":"Near-infrared spectroscopy: application in ensuring food quality and safety","authors":"Yuxia Xu, Tianyu Kong, Yinfei Ma, Yan Zhao, Le Chu and Mingzhu Zheng","doi":"10.1039/D4AY02039A","DOIUrl":"https://doi.org/10.1039/D4AY02039A","url":null,"abstract":"<p >In recent years, the demand for intelligent control of food quality during processing has been increasing in the food industry. As a practical analytical tool, near-infrared (NIR) spectroscopy has become a common detection method to ensure food quality and safety because of its advantages of continuous, rapid on-line determination and strong analytical performance. In the past 20 years, many attempts and research studies have been conducted on the applications of NIR spectroscopy. Based on this, this review focuses on the specific application of near-infrared technology in the field of food, highlighting its breakthrough and applicability. NIR spectroscopy is widely used for online quantitative analysis of beneficial food components to the human body, which include proteins, polysaccharides, and polyphenols. Additionally, this technology is applied to food microbiological analysis, food safety detection (such as food adulteration), and food origin prediction. This review discusses the existing challenges, future development directions, and opportunities for NIR spectroscopy technology.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3381-3406"},"PeriodicalIF":2.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Peptide–DNA conjugate-assisted DNA cyclization for highly efficient and sensitive detection of furin† 肽- DNA缀合物辅助DNA环化高效灵敏地检测furin†
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-22 DOI: 10.1039/D5AY00370A
Minghui Wang, Cuifei Chen, Keqin Ying, Lingjun Sha, Deqi Kuang, Yue Huang, Jie Yang and Tingting Yin
{"title":"Peptide–DNA conjugate-assisted DNA cyclization for highly efficient and sensitive detection of furin†","authors":"Minghui Wang, Cuifei Chen, Keqin Ying, Lingjun Sha, Deqi Kuang, Yue Huang, Jie Yang and Tingting Yin","doi":"10.1039/D5AY00370A","DOIUrl":"https://doi.org/10.1039/D5AY00370A","url":null,"abstract":"<p >Furin, a critical regulatory protease, plays a crucial role in significant physiological processes such as oncogenic signaling pathways. Accurate detection of furin is essential for disease diagnosis and precise medical interventions. Herein, we leverage the proximity effect of the engineered peptide–DNA conjugate (PDC) to induce DNA cyclization, enabling efficient and sensitive detection of furin. The PDC is synthesized through chemical reactions that link peptides and DNA molecules, strategically structured with a peptide moiety containing the specific recognition and cleavage site for furin, sandwiched between two DNA sequences. Meanwhile, the proximity effect-mediated DNA hybridization of the PDC achieves the complementation of Padlock DNA, thereby ensuring circular DNA formation. Subsequently, the cyclized DNA serves as a template to initiate the rolling circle amplification reaction, generating numerous G-quadruplex structures that yield a fluorescence signal upon thioflavin T intercalation. While furin-catalyzed peptide cleavage disrupts the proximity effect, DNA cyclization and subsequent reactions are inhibited. The multifunctional PDC probe which can assist DNA cyclization facilitates furin recognition through peptide embedding, thus providing a new way for sensitive detection of furin, with a detection limit of 1.5 pM. Additionally, our method demonstrates efficacy in inhibitor determination, showing great potential across biomedical research, clinical diagnostics, and therapeutic development.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3570-3578"},"PeriodicalIF":2.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research on chemical constituent differences in wild and cultivated Arnebia euchroma by combining UPLC-QTOF-MS with chemometrics† UPLC-QTOF-MS与化学计量学相结合研究野生与栽培紫草的化学成分差异
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-22 DOI: 10.1039/D4AY01998A
R. Huang, D. X. Wu, R. Wu, N. Yang, F. Wei, S. Y. Dai and J. Zheng
{"title":"Research on chemical constituent differences in wild and cultivated Arnebia euchroma by combining UPLC-QTOF-MS with chemometrics†","authors":"R. Huang, D. X. Wu, R. Wu, N. Yang, F. Wei, S. Y. Dai and J. Zheng","doi":"10.1039/D4AY01998A","DOIUrl":"https://doi.org/10.1039/D4AY01998A","url":null,"abstract":"<p >In this study, ultra-high performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC-QTOF-MS) was used to analyze the wild and cultivated <em>Arnebia euchroma</em> and the chemical constituent differences were determined by chemometric analysis including principal component analysis (PCA), hierarchical cluster analysis (HCA), and orthogonal partial least squares-discrimination analysis (OPLS-DA). The MS data were processed using UNIFI combined with an in-house library to characterize the metabolites automatically. Based on the multiple adduct ions, exact mass, diagnostic fragment ions, peak intensity, retention time of compounds, and the fragmentation mechanism and retention behavior of references, the structures identified by UNIFI were further verified and the unidentified compounds were also tentatively elucidated. 68 compounds were identified, including 26 shikonins, 14 shikonofurans, 7 flavonoids, 7 phenolic acids, 5 esters, 2 phenylpropanoids, 2 saccharides and 5 others. The chemometrics results showed that the compositions of wild and cultivated <em>Arnebia euchroma</em> were significantly different; 8 differential components were obtained through VIP &gt;1 screening. The quality of wild and cultivated products is strongly influenced by altitude, and the higher the altitude, the more favorable the accumulation of chemical constituents, especially shikonofurans. These results provide basic information for revealing the effects of the ecological environment on the metabolite synthesis and accumulation of <em>Arnebia euchroma</em> and provide an important basis for further research and clinical application of wild and cultivated <em>Arnebia euchroma</em>.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3579-3589"},"PeriodicalIF":2.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Waterborne protozoan parasite detection using two-frequency impedance flow cytometry† 双频阻抗流式细胞术检测水生原生动物寄生虫
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-22 DOI: 10.1039/D5AY00184F
Yunhao Peng, Bruce K. Gale and Himanshu J. Sant
{"title":"Waterborne protozoan parasite detection using two-frequency impedance flow cytometry†","authors":"Yunhao Peng, Bruce K. Gale and Himanshu J. Sant","doi":"10.1039/D5AY00184F","DOIUrl":"https://doi.org/10.1039/D5AY00184F","url":null,"abstract":"<p >Waterborne parasitic protozoa are common causes of gastrointestinal diseases in both humans and farm animals, even with standardized water treatment in place. This creates a need for continuous water monitoring to detect the presence of these micron-sized parasites in water sources to prevent potential outbreaks. This paper proposes a monitoring system consisting of a microfluidic channel embedded with micromachined microelectrodes to detect and evaluate protozoa at the individual (oo)cyst level in flowing water. To identify and discriminate between the (oo)cysts of Giardia and Cryptosporidium, two frequency-based impedance flow cytometry (IFC) is employed, where a high and a low frequency are applied to obtain the amplitude and phase variances of the samples. Using combination of amplitude and phase measurements at tested frequencies, the parasites and non-parasites (polystyrene) are identified, and a high degree of discrimination is also demonstrated for samples suspended in both DI water and filtered creek water. While impedance flow cytometry was utilized to detect waterborne protozoa, the system proposed in this paper is distinctive in the following ways. It employs differential coplanar electrodes instead of parallel electrodes to achieve a limit detection of &lt;0.1% volume ratio between volume of a single (oo)cyst and the volume the electrodes occupy in the channel. It applies a low and high frequency simultaneously to obtain amplitude ratios to characterize sample populations instead of amplitude <em>vs.</em> phase at a single fixed frequency, potentially improving sample discrimination. This work also demonstrates detection and identification of protozoan (oo)cysts suspended in a natural water source, instead of in purified water, showing the proposed system's potential as a continuous waterborne parasitic protozoa monitoring system in a real environment.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3590-3599"},"PeriodicalIF":2.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d5ay00184f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dual-emission copper nanoclusters for hydrogen sulfide detection: a novel approach† 双发射铜纳米簇用于硫化氢检测:一种新方法
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-17 DOI: 10.1039/D5AY00487J
Longyan Wang, Huachao Che, Lizhu Fang, Liqiang Lu, Xike Tian and Yeukuang Hwu
{"title":"Dual-emission copper nanoclusters for hydrogen sulfide detection: a novel approach†","authors":"Longyan Wang, Huachao Che, Lizhu Fang, Liqiang Lu, Xike Tian and Yeukuang Hwu","doi":"10.1039/D5AY00487J","DOIUrl":"https://doi.org/10.1039/D5AY00487J","url":null,"abstract":"<p >Dual-emission copper nanoclusters (CuNCs) were synthesized <em>via</em> a novel, green and one-pot aqueous method, employing bovine serum albumin and glutathione as reducing and stabilizing agents, respectively. The CuNCs exhibited distinct fluorescence emissions at 443 nm and 612 nm under 365 nm excitation. Notably, with the increase in hydrogen sulfide concentration, the dual-emission fluorescence of CuNCs was gradually quenched, and complete quenching occurred at 280 μM. The probe demonstrated a stable response to hydrogen sulfide within the concentration range of 2.5–60.0 μM. The detection limit of hydrogen sulfide was as low as 0.32 μM, which is significantly lower than the limit set by the World Health Organization (1.5 μM). This method was successfully applied for the detection of sulfide in environmental samples, achieving remarkable recovery rates ranging from 98.2–102%. Moreover, the response mechanism and practical application potential of CuNCs as a hydrogen sulfide detection platform were investigated, offering crucial insights for the study of fluorescence properties of metal nanoclusters and the prevention and control of hydrogen sulfide pollution.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3561-3569"},"PeriodicalIF":2.7,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A photonic crystal-hydrogel sensor for the monitoring of potassium ions in sweat 用于监测汗液中钾离子的光子晶体-水凝胶传感器
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-16 DOI: 10.1039/D5AY00383K
Bing Lin, Irfan Muhammad, Wenxin Zhang, Yifei Wang and Zihui Meng
{"title":"A photonic crystal-hydrogel sensor for the monitoring of potassium ions in sweat","authors":"Bing Lin, Irfan Muhammad, Wenxin Zhang, Yifei Wang and Zihui Meng","doi":"10.1039/D5AY00383K","DOIUrl":"https://doi.org/10.1039/D5AY00383K","url":null,"abstract":"<p >Sweat contains abundant physiological information critical for health monitoring, making wearable sweat sensors increasingly popular in exercise and training surveillance. Most of the existing sensors are designed to measure physical parameters such as heart rate, blood pressure, and respiration, relying on electronic instruments for biochemical analysis of body fluids. In this study, we present a wearable hydrogel band integrated with a photonic crystal array, for visual, non-invasive and <em>in situ</em> analysis of sweat for sports practitioners. The hydrogel sensor, inspired by the biological properties of onions, demonstrates excellent skin adhesion for efficient sweat collection. This innovative design allows for the development of a simple, reliable, and low-cost sensor capable of detecting potassium ion concentrations and pH levels in sweat. Under optimized conditions, the developed sensor can respond to potassium ion concentrations ranging from 1 mM to 150 mM, with a pH response range of 3 to 9 for human sweat. The dynamic cycling tests of potassium ion concentration and pH in sweat demonstrated that this sensor can effectively monitor electrolyte balance and dehydration during outdoor exercise.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3547-3553"},"PeriodicalIF":2.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Development of a fluorous trapping reagent for rapid detection of electrophilic reactive metabolites 更正:开发用于快速检测亲电反应代谢物的含氟捕获试剂
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-15 DOI: 10.1039/D5AY90040A
Yusuke Akagi, Hiroyuki Yamakoshi and Yoshiharu Iwabuchi
{"title":"Correction: Development of a fluorous trapping reagent for rapid detection of electrophilic reactive metabolites","authors":"Yusuke Akagi, Hiroyuki Yamakoshi and Yoshiharu Iwabuchi","doi":"10.1039/D5AY90040A","DOIUrl":"https://doi.org/10.1039/D5AY90040A","url":null,"abstract":"<p >Correction for ‘Development of a fluorous trapping reagent for rapid detection of electrophilic reactive metabolites’ by Yusuke Akagi <em>et al.</em>, <em>Anal. Methods</em>, 2024, <strong>16</strong>, 3810–3814, https://doi.org/10.1039/D4AY00577E.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3600-3600"},"PeriodicalIF":2.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d5ay90040a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A portable paper-based sensor for simultaneous determination of sodium and potassium ions in the human plasma matrix 用于同时测定人血浆基质中钠离子和钾离子的便携式纸基传感器
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-14 DOI: 10.1039/D5AY00234F
Mohammed Alqarni
{"title":"A portable paper-based sensor for simultaneous determination of sodium and potassium ions in the human plasma matrix","authors":"Mohammed Alqarni","doi":"10.1039/D5AY00234F","DOIUrl":"https://doi.org/10.1039/D5AY00234F","url":null,"abstract":"<p >Accurate determination of sodium and potassium ions in biological fluids is crucial for managing various health conditions, as these electrolytes play a vital role in regulating physiological processes and maintaining overall well-being. A low-cost portable paper-based sensor has been developed for the simultaneous determination of sodium and potassium in the human plasma matrix. It comprises three electrodes on a single paper substrate: a solid-state polyvinyl butyral reference electrode, a sodium selective electrode and a potassium selective electrode. The sensor utilizes conductive functionalized multi-walled carbon nanotubes, selective ionophores and dyes to enhance sensing capabilities. It demonstrates high selectivity for sodium and potassium ions, with a linear range of 10<small><sup>−6</sup></small> to 10<small><sup>−2</sup></small> M and a Nernstian response (slopes of 59.08 ± 0.32 and 59.15 ± 0.28 mV per decade for sodium and potassium, respectively). The sensor provides a reproducible stable potential over 180 days and a dynamic response time of 10 s. The described paper-based sensor is an excellent tool for point-of-care analysis of sodium and potassium imbalances due to its simplicity, cost-effectiveness and disposability. It provides fast and reliable determination of sodium and potassium ions in the human plasma matrix, making it a valuable tool for managing their imbalances in clinical settings.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3499-3508"},"PeriodicalIF":2.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sensitive gas spectroscopy method for real-time determination of urease activity via ammonia production† 灵敏气相光谱法实时测定氨生成脲酶活性†
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-14 DOI: 10.1039/D4AY02270J
Letícia Andrade Simões Lopes, Dominik Clara Luz Lopes, Leonardo Mota, Guilherme Rodrigues Lima, Bruno José Rodrigues Alves and Marcelo Gomes da Silva
{"title":"Sensitive gas spectroscopy method for real-time determination of urease activity via ammonia production†","authors":"Letícia Andrade Simões Lopes, Dominik Clara Luz Lopes, Leonardo Mota, Guilherme Rodrigues Lima, Bruno José Rodrigues Alves and Marcelo Gomes da Silva","doi":"10.1039/D4AY02270J","DOIUrl":"https://doi.org/10.1039/D4AY02270J","url":null,"abstract":"<p >In this study we propose the use of a laser-based photoacoustic spectrometer as a new method to investigate the kinetics of ammonia (NH<small><sub>3</sub></small>) emission from the hydrolysis of urea (H<small><sub>2</sub></small>N–CO–NH<small><sub>2</sub></small>) in the presence of urease. Experiments explored the effects of varying the amounts of water and urease on nitrogen (N) loss from urea aqueous solutions. A linear increase in N–NH<small><sub>3</sub></small> loss and the maximum emission rate (<em>r</em><small><sub>NH<small><sub>3</sub></small></sub></small>) was observed with increasing certified urease concentrations. Specifically, the addition of 10 units of urease resulted in an N loss of 8.1%, with a sensitivity of approximately 0.8% per unit of urease, and reached 12.8 μmol min<small><sup>−1</sup></small> with a sensitivity of 1.1 μmol min<small><sup>−1</sup></small> per unit of urease for <em>r</em><small><sub>NH<small><sub>3</sub></small></sub></small>. These relationships enabled the estimation of urease quantities in commercial soy flour extracts. Variations in water and urease proportions revealed that maximum NH<small><sub>3</sub></small> emissions occurred within the first 2–5 h, with the highest N–NH<small><sub>3</sub></small> loss value attaining (9 ± 1)% for samples containing 2.0 mL soy flour extracts and additional water. For urease concentration assays, N–NH<small><sub>3</sub></small> loss and <em>r</em><small><sub>NH<small><sub>3</sub></small></sub></small> were (14.4 ± 0.1)% and (25.3 ± 0.1) μmol min<small><sup>−1</sup></small>, respectively, with 4.0 mL of soy flour extract. The results underscored the dominant influence of urease compared to that of water in urea hydrolysis. The PA spectrometer demonstrated sufficient sensitivity for detecting NH<small><sub>3</sub></small>, rendering it a promising tool for studying urease activity in urea decomposition. Future work could explore this system under crop field conditions to elucidate the roles of urease and water in the cycling of nutrients within agroecosystems.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3490-3498"},"PeriodicalIF":2.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A label-free aptasensor based on a fluorescent probe containing styrylbenzothiazole for detection of enrofloxacin 基于苯基苯并噻唑荧光探针的无标记适配体传感器检测恩诺沙星
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-11 DOI: 10.1039/D4AY02265C
Wei-Chuang Kong, Chen-Chen Li, Jun-Tao Niu, Xu-Ying Liu, Dong Wei and Yan-Fei Kang
{"title":"A label-free aptasensor based on a fluorescent probe containing styrylbenzothiazole for detection of enrofloxacin","authors":"Wei-Chuang Kong, Chen-Chen Li, Jun-Tao Niu, Xu-Ying Liu, Dong Wei and Yan-Fei Kang","doi":"10.1039/D4AY02265C","DOIUrl":"https://doi.org/10.1039/D4AY02265C","url":null,"abstract":"<p >Enrofloxacin (ENR), a third-generation fluoroquinolone antibiotic, poses significant health risks due to potential drug residues in the food chain, necessitating effective monitoring methods. Here, we synthesized a G-quadruplex-targeted fluorescent probe PBTZM containing styrylbenzothiazole. Using this probe, we successfully developed an aptasensor for the detection of ENR by combining it with an ENR aptamer. Under optimal conditions, the aptasensor provided a linear range of 0.001–5 μM and a detection limit of 24.74 nM. In addition, this method showed good performance for ENR detection in river water, milk, serum and urine samples and was further validated by standard HPLC techniques without a significant difference (<em>p</em> &gt; 0.05). It will have great potential in advancing research of quinolone antibiotics and the development of aptamer-based biosensing technologies.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3471-3479"},"PeriodicalIF":2.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信