{"title":"The visual detection of amoxicillin using a dual-mode probe based on CQD-doped MnO<sub>2</sub> nanospheres.","authors":"Zhenhua Wu, Nani Fu, Xianqin Han, Ting Yan, Jiaying Zheng, Jintao Yi","doi":"10.1039/d5ay01256b","DOIUrl":"https://doi.org/10.1039/d5ay01256b","url":null,"abstract":"<p><p>Amoxicillin (AMO), a broad-spectrum penicillin antibiotic, has been widely employed for the treatment of bacterial infections. However, the unreasonable use of amoxicillin may cause a serious threat to human health, and its visual determination remains a challenge. Herein, a MnO<sub>2</sub>@CQD probe was used for the dual-mode detection of AMO, which was synthesized by doping CQDs into MnO<sub>2</sub> nanospheres. In the colorimetric mode, MnO<sub>2</sub>@CQDs oxidized colorless 3, 3', 5, 5'-tetramethylbenzidine (TMB) into yellow oxidized TMB (oxTMB). In the presence of AMO, AMO could deoxidize oxTMB to cause multiple color changes from yellow to colorless. In the fluorescence mode, MnO<sub>2</sub>@CQDs initially exhibited quenched fluorescence due to the presence of MnO<sub>2</sub>. Upon the addition of AMO, MnO<sub>2</sub> was reduced to Mn<sup>2+</sup> ions, and then CQDs were released and their fluorescence was recovered at an emission wavelength of 450 nm. The colorimetric detection range was wide from 0.005 to 0.09 μM, and the detection limit was 1.6 nM. With increasing concentration of AMO, the color changed from yellow to colourless, which allowed naked eye observation. The fluorescence method was rapid, requiring only 1 min, and exhibited a linear detection range from 0.003 to 0.045 μM, with a detection limit as low as 1 nM. In addition, a paper-based sensing platform was constructed, where fluorescence intensity transitioned from dark to blue with increasing AMO levels. The RGB analysis demonstrated that AMO concentration could be discriminated effectively using a smartphone-assisted detection system. The MnO<sub>2</sub>@CQD sensor showed excellent selectivity and specificity for AMO colorimetric and fluorescence detection. Moreover, the probe exhibited good practicability in tap water, milk and serum, with results comparable to those obtained by high-performance liquid chromatography. Overall, the MnO<sub>2</sub>@CQD dual-mode probe provides an efficient, convenient and rapid strategy for the visual and quantitative detection of AMO in complex environments.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145204953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yingzi Wang, Chen Zhao, Shang Wang, Bin Xue, Chenyu Li, Xi Zhang, Xiaobo Yang, Zhigang Qiu, Zhiqiang Shen, Jingfeng Wang
{"title":"A sensitive quantitative detection technology for tetracycline and erythromycin based on allosteric transcription factors (aTFs) using SPR.","authors":"Yingzi Wang, Chen Zhao, Shang Wang, Bin Xue, Chenyu Li, Xi Zhang, Xiaobo Yang, Zhigang Qiu, Zhiqiang Shen, Jingfeng Wang","doi":"10.1039/d5ay01317h","DOIUrl":"https://doi.org/10.1039/d5ay01317h","url":null,"abstract":"<p><p>Antibiotics are extensively utilized in agriculture and livestock farming; however, their environmental residues may foster the proliferation of drug-resistant bacteria and disrupt ecosystems, underscoring the necessity for sensitive detection technologies. Allosteric transcription factors (aTFs) represent a class of proteins that can specifically recognize small molecules and regulate gene expression. Owing to their high specificity and sensitivity, aTFs have been widely adopted in the development of biosensors. This study utilized the properties of allosteric transcription factors to alter their DNA affinity upon ligand binding. By measuring the changes in affinity of TetR and MphR before and after DNA binding using SPR, the study quantitatively analyzed the concentrations of tetracycline and erythromycin. Through SPR technology, quantitative detection of these affinity changes was achieved. Under optimized conditions regarding sample incubation time and system injection time, the affinity values exhibited changes of two orders of magnitude. The detection limits for tetracycline and erythromycin using this technique were determined to be 0.8 nM and 1 nM, respectively. These results indicate that the developed SPR detection method demonstrates high sensitivity and accuracy for detecting tetracycline and erythromycin, making it a promising tool for various applications, including environmental monitoring and quality control.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Emerging nanosensor technologies for the rapid detection of heavy metal contaminants in agricultural soils.","authors":"Anyou Xie, Weihong Wu","doi":"10.1039/d5ay01248a","DOIUrl":"https://doi.org/10.1039/d5ay01248a","url":null,"abstract":"<p><p>The accumulation of heavy metals in agricultural soils presents a growing threat to food safety and human health. Conventional laboratory-based methods for heavy metal detection, while highly sensitive, are impractical for widespread, real-time soil monitoring due to their cost, complexity, and lack of portability. In response, nanosensor technologies have emerged as promising alternatives, offering rapid, sensitive, and field-deployable detection platforms. This review critically examines the current landscape of electrochemical and optical nanosensors engineered for trace metal analysis in soil environments. Electrochemical platforms, particularly those employing nanomaterial-modified screen-printed electrodes and anodic stripping voltammetry, demonstrate excellent sensitivity and portability, though challenges remain in achieving selectivity and mitigating matrix interference. Optical nanosensors, including colorimetric, fluorescent, and surface-enhanced Raman scattering (SERS)-based systems, offer diverse mechanisms of detection and ultralow detection limits but are often limited by soil turbidity, biofouling, and substrate instability. Particular attention is given to the integration of advanced materials such as metal-organic frameworks, carbon-based nanomaterials, and \"green\" quantum dots, which aim to improve sensor performance and environmental safety. The review also explores the convergence of nanosensor deployment with data science, emphasizing the role of machine learning in signal deconvolution, calibration, and real-time decision support. Finally, the article highlights future directions, including the development of anti-fouling coatings, sustainable fabrication methods, multiplexed detection arrays, and sensor networks integrated <i>via</i> the Internet of Things. Together, these advances suggest a pathway toward robust, intelligent, and scalable soil monitoring systems critical for precision agriculture and environmental stewardship.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Critical perspectives on electrochemical biosensing strategies for non-invasive lactate detection in athletic performance monitoring.","authors":"Qinhai Wang, Tao Li","doi":"10.1039/d5ay00941c","DOIUrl":"https://doi.org/10.1039/d5ay00941c","url":null,"abstract":"<p><p>Non-invasive monitoring of lactate levels offers a promising avenue for optimizing athletic performance assessment, yet remains constrained by the limitations of traditional blood-based sampling methods. This review critically examines electrochemical biosensing strategies for non-invasive lactate detection tailored to sports applications. Emphasis is placed on enzymatic, non-enzymatic, and molecularly imprinted polymer (MIP)-based approaches, each with distinct strengths and limitations in sensitivity, specificity, and operational stability. The suitability of various biofluids-sweat, saliva, and interstitial fluid (ISF)-is evaluated with regard to physiological relevance, correlation with blood lactate, and challenges in sampling and sensor integration. Particular attention is given to the physiological mechanisms governing lactate appearance in these fluids and their implications for real-time monitoring. Advanced materials such as nanostructured metal oxides, conductive polymers, and MOFs are discussed for their roles in enhancing electrocatalytic performance and biosensor durability. Despite significant advances, unresolved challenges persist, including weak or inconsistent correlation between biofluid and blood lactate, sensor biofouling, lag times in ISF detection, and insufficient validation in real-world athletic settings. The review highlights emerging strategies such as multiparametric sensing, machine learning models, and innovative sensor designs (<i>e.g.</i>, microneedles, three-phase interfaces) as potential solutions to bridge these gaps. Future progress will depend on integrating physiological insight with materials innovation and rigorous validation to develop robust, field-deployable lactate biosensors that can transform athlete monitoring and training personalization.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eric Asare, Ting Du, Yuan Chen, Shalom Emmanuel, Huan Xie, Dong Liang, Song Gao
{"title":"Development and validation of a novel LC-MS/MS method for simultaneous quantification of alvocidib and its glucuronide: application to pharmacokinetic and tissue distribution study in rats.","authors":"Eric Asare, Ting Du, Yuan Chen, Shalom Emmanuel, Huan Xie, Dong Liang, Song Gao","doi":"10.1039/d5ay01143d","DOIUrl":"https://doi.org/10.1039/d5ay01143d","url":null,"abstract":"<p><p>Alvocidib, an approved drug for the treatment of acute myeloid leukemia, undergoes glucuronidation metabolism. The purpose of this study is to develop a robust and sensitive LC-MS/MS method to simultaneously quantify alvocidib and its metabolite, alvocidib-glucuronide (Alv-G), and apply this method in a pharmacokinetic and tissue distribution study in rats. A UHPLC system coupled to an AB Sciex QTrap 4000 triple quadrupole mass spectrometer was used for the analysis. The analytes were separated using a Biphenyl column with 0.1% formic acid in water and acetonitrile as the mobile phases and were quantified under positive electrospray ionization (ESI) mode using a multiple reaction monitoring (MRM) approach. Protein precipitation with acetonitrile was used in sample processing. The method showed a good linearity within the range of 2.4-5000.00 nM for both analytes. The method was reproducible, with intra and inter-day accuracy and precision ranging from 84-98% and <13% respectively. The extraction recovery and matrix effect achieved acceptable ranges (82-105%). Stability studies demonstrated that they were stable under bench-top (91-99%), freeze-thaw (83-110%), and long-term storage conditions (80-106%). The PK results showed that after oral administration of alvocidib, both alvocidib and Alv-G were detected in plasma with the parent drug showing highest distribution in the colon, and lowest in the small intestine. In contrast, Alv-G showed the highest distribution in the small intestine and the lowest amount in the colon compared to the other tissues. This method is applicable for PK and tissue distribution studies to quantify both alvocidib and its glucuronide.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Wearable sensors for monitoring workplace chemical exposures in occupational health management.","authors":"Haibin Liu, Zhili Wang, Gao Zhou","doi":"10.1039/d5ay00961h","DOIUrl":"https://doi.org/10.1039/d5ay00961h","url":null,"abstract":"<p><p>This review evaluates the current state of wearable chemical sensors for occupational health applications, emphasizing both their promise and their limitations. We systematically compare electrochemical, optical, photoionization, and chemiresistive platforms in terms of detection limits, selectivity, drift behavior, and calibration requirements. Evidence from field trials-such as the performance of electrochemical CO and H<sub>2</sub>S monitors in mining and firefighting and the variable accuracy of portable infrared analyzers for anesthetic gases in healthcare-illustrates both successful deployments and persistent challenges including calibration drift, power demands, and user acceptance. Beyond analytical performance, practical factors such as device weight, comfort, battery life, and data privacy compliance emerge as critical determinants of adoption. We further examine integration into occupational workflows, highlighting how calibration and quality assurance routines, interoperability with occupational health systems, and ethical frameworks for worker consent shape real-world utility. While significant progress has been achieved through advances in materials science, wireless communication, and machine learning-enabled analytics, the lack of wearable-specific validation standards (ISO, ASTM, and NIOSH) continues to impede regulatory acceptance. Future research should therefore prioritize robust field validation, development of consensus performance benchmarks, and hybrid sensing architectures that balance sensitivity, selectivity, and usability. Taken together, this review positions wearable chemical sensors as a promising but still maturing tool for proactive occupational exposure monitoring, requiring continued refinement before widespread regulatory adoption.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Simple preparation of an Au nanoparticle dispersed substrate by arc plasma deposition and its application in bio-sensing using LSPR.","authors":"Masahiro Takebayashi, Koyo Kawabata, Masahiro Yasukawa, Reo Tanaka, Shota Kawachi, Tomohiro Shimizu, Shoso Shingubara, Takeshi Ito","doi":"10.1039/d5ay00827a","DOIUrl":"https://doi.org/10.1039/d5ay00827a","url":null,"abstract":"<p><p>We propose a simple preparation of a Localized Surface Plasmon Resonance (LSPR) device in which Au nanoparticles (AuNPs) are dispersed on SiO<sub>2</sub>/Si substrates using arc plasma deposition (APD). APD can coat AuNPs directly onto a substrate using a single-step process with a very short deposition time. The dispersion and diameter of the AuNPs were evaluated based on the APD characteristics: applied voltage (<i>V</i>), capacitance (<i>C</i>), and number of pulses (<i>n</i>). The LSPR device was prepared under the best conditions for reflectance spectra: <i>C</i> = 2200 μF, <i>V</i> = 100 V, <i>n</i> = 80, and a vacuum of 1.3 × 10<sup>-3</sup> Pa at room temperature, and protein-protein interactions (PPIs) were detected using the device. The fabricated device could detect avidin over a wide range from 1 ng mL<sup>-1</sup> to 10 μg mL<sup>-1</sup>. The detection limit was 0.33 ng mL<sup>-1</sup>, which is considerably low compared to that of other detection methods. These results demonstrate that APD is useful for fabricating superior nanoparticles without additional processes such as photolithography and etching.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meng Liu, Shuaipeng Li, Chaolin Guo, Ziyan Xie, Qingxuan Miao, Yankun Yang, Jianqi Nie, Yang Sun, Zhonghu Bai
{"title":"A novel rapid single-integrated immunoassay for plasma thrombomodulin: analytical performance and multi-center clinical validation.","authors":"Meng Liu, Shuaipeng Li, Chaolin Guo, Ziyan Xie, Qingxuan Miao, Yankun Yang, Jianqi Nie, Yang Sun, Zhonghu Bai","doi":"10.1039/d5ay01241d","DOIUrl":"https://doi.org/10.1039/d5ay01241d","url":null,"abstract":"<p><p>Thrombotic diseases have emerged as a significant public health issue, with timely detection, diagnosis, and treatment being closely associated with patient prognosis. Consequently, the identification of highly sensitive and specific biochemical markers for thrombotic diseases holds substantial clinical importance. In this study, we developed a rapid, single-integrated quantitative detection method for thrombomodulin (TM). This method integrates all reagent components onto a single, sophisticated card strip, enabling the completion of the assay in merely 20 minutes. Such efficiency enhances the potential for early screening of thrombosis within community and primary care medical institutions. The analytical performance results indicate that the limit of blank (LoB), limit of detection (LoD), and limit of quantitation (LoQ) were determined to be 0.5, 0.8, and 1.2 TU mL<sup>-1</sup>, respectively. The repeatability and precision of the method were found to be significantly below 10%, with a linear range extending from 1.2 to 200 TU mL<sup>-1</sup>. Accuracy evaluations revealed relative biases of -3.35% and 4.79% for the two reference materials tested. Furthermore, clinical trials conducted across two clinical research centers involved a total of 236 clinical plasma samples. The results of the clinical analysis demonstrated excellent consistency with the reference reagent (Sysmex). The receiver operating characteristic (ROC) curve and significance analysis indicated that this method possesses commendable specificity and sensitivity. In summary, the single-integrated immunoassay method developed in this study represents a rapid, convenient, accurate, and sensitive detection approach. It is particularly well-suited for the early screening and diagnosis of thrombotic diseases in community and primary healthcare institutions.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kaitlyn Allen, Gene Ahlborn, Julia Wangsgard, Shintaro Pang
{"title":"Application of surface enhanced Raman spectroscopy (SERS) for the rapid detection and enumeration of probiotics in yogurt.","authors":"Kaitlyn Allen, Gene Ahlborn, Julia Wangsgard, Shintaro Pang","doi":"10.1039/d5ay00931f","DOIUrl":"https://doi.org/10.1039/d5ay00931f","url":null,"abstract":"<p><p>Probiotics are a critical component in fermented dairy products such as yogurt. An efficient method to enumerate probiotics in commercially produced yogurt is important, but traditional methods are time-consuming, sometimes taking up to 72 hours. The objective of this study was to develop a rapid method using surface-enhanced Raman spectroscopy (SERS) to identify and enumerate probiotic bacterial cells in yogurt. The identification and enumeration of three different probiotic bacterial strains, <i>Lactobacillus acidophilus</i>, <i>Lactobacillus bulgaricus</i>, and <i>Streptococcus thermophilus</i> were successfully conducted using a developed SERS protocol. <i>L. bulgaricus</i> and <i>S. thermophilus</i> were also identified from a yogurt matrix using a simple extraction procedure utilizing a Percoll gradient. Statistical spectral analysis including principal component analysis and partial least squares regression analysis allowed for the differentiation, characterization and quantification of probiotic strains. The limits of detection were 10<sup>5</sup> CFU mL<sup>-1</sup> for <i>L. acidophilus</i>, 10<sup>4</sup> CFU mL<sup>-1</sup> for <i>L. bulgaricus</i>, and 10<sup>6</sup> CFU mL<sup>-1</sup> for <i>S. thermophilus</i>. Quantification capability was tested up to 10<sup>8</sup> cfu mL<sup>-1</sup> and showed strong correlation coefficients (0.94, 0.98 and 0.95 respectively) between predicted and actual concentrations. The presence of probiotic mixtures were clearly detectable from a yogurt food matrix starting at 10<sup>5</sup> CFU mL<sup>-1</sup>. Sample analysis took less than three hours per sample set. Our results demonstrate the capability of SERS to detect and enumerate probiotics from a yogurt matrix rapidly.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Colorimetric and ratiometric fluorescent probes for sensitive visualization of hydrazine in water and in living cells.","authors":"Weijie Chang, Wenyi Yang, Xiaoxiao Wang, Fanglong Xu, Weibing Zhang, Junhong Qian","doi":"10.1039/d5ay01337b","DOIUrl":"https://doi.org/10.1039/d5ay01337b","url":null,"abstract":"<p><p>Taking account of the strong nucleophilicity of hydrazine, two fluorescent probes, DEA-A and Jul-B, were designed and synthesized. These probes employed 2,3,5,6-1<i>H</i>,4<i>H</i>-tetrahydroquinolo[9,9a,1-gh]coumarin (Jul-B) or <i>N</i>,<i>N</i>-diethyl-7-aminocoumarin (DEA-A) as the fluorophore, and 2-benzothiazole acetonitrile as the recognition moiety for N<sub>2</sub>H<sub>4</sub>. The formation of hydrazones weakened the intramolecular charge transfer (ICT) process in the probes, leading to blue-shifts of more than 100 nm in both absorption and emission. DEA-A and Jul-B demonstrated notable colorimetric and ratiometric fluorescence responses to N<sub>2</sub>H<sub>4</sub>, which enabled us to realize the identification of hydrazine using the \"naked-eye\". In the case of DEA-A, the <i>I</i><sub>513</sub>/<i>I</i><sub>616</sub> ratio exhibits a linear relationship with N<sub>2</sub>H<sub>4</sub> concentration in the range of 1.0-150 μM, and the detection limit for N<sub>2</sub>H<sub>4</sub> was determined to be 0.10 μM. With the assistance of smartphones, N<sub>2</sub>H<sub>4</sub> concentrations in realistic samples were visualized <i>via</i> RGB image analysis. In addition, both probes were applied in ratiometric fluorescence imaging of N<sub>2</sub>H<sub>4</sub> in the living L929 cell line.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}