Analytical Methods最新文献

{"title":"Removal and measurement of trace amounts of rhodamine B in aqueous samples based on the synthesis of a nanosorbent composed of Fe₃O₄ nanoparticles modified with SiO₂ and polydopamine by magnetic solid phase extraction.","authors":"Abdollah Yari, Mariam Salemzadeh","doi":"10.1039/d4ay01537a","DOIUrl":"https://doi.org/10.1039/d4ay01537a","url":null,"abstract":"<p><p>Rhodamine B (RDB) dye is generally toxic and causes problems such as carcinogenic activities, neurological disorders, and respiratory tract irritations. However, some individuals still illegally use RDB as a food additive. Therefore, a simple and accurate method is needed to determine RDB in real samples such as food or cosmetic products. In this study, the magnetic solid phase extraction (MSPE) method was used to measure very low amounts of RDB dye in water samples. The advantages of this method include simplicity, good repeatability, high preconcentration factor, higher extraction efficiency, low organic solvent volume requirement, rapid separation, low cost, high sensitivity, and interference removal compared to other methods. Within this approach, iron oxide nanoparticles were synthesised, followed by the application of tetra ethoxy silane (TEOS) and polydopamine (PDA) coatings on the nanoparticle surface, resulting in the creation of the Fe₃O₄@SiO₂@PDA nano-sorbent. This nano-sorbent was then utilized as the magnetic solid phase in MSPE. The characteristics of the synthesized compounds were investigated by various methods such as infrared Fourier transform spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction spectroscopy (XRD), and vibrating sample magnetometry (VSM). Response surface methodology (RSM) and Box-Behnken (BB) design were used to optimize the variables affecting the adsorption process and achieved high recovery percentages. Under optimal experimental conditions, the detection limit and quantification limit of the method were found to be 0.73 and 2.42 μg L^-1 for RDB, respectively, with RSDs of ±0.32% and ±0.67%. Furthermore, the linear concentration range of the proposed method for solid phase extraction of RDB was determined to be 10.0-900.0 μg L^-1. Moreover, the recovery rates for tap water, lake water, and well water samples ranged from 96.9% to 98.6%.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a metabolomics method in the study of pear fruit storage.","authors":"Jing Liu, Zixuan Meng, Baoru Liu, Jing Wang, Haichao Zhang, Lianfeng Ai, Junfeng Guan, Lingmei Niu","doi":"10.1039/d4ay01368a","DOIUrl":"https://doi.org/10.1039/d4ay01368a","url":null,"abstract":"<p><p>The entire transportation process of 'crown' pears from harvest to consumption is primarily refrigerated. However, the impact of refrigeration time and temperature on fruit quality remains unclear. In this study, metabolites of 'crown' pears were analyzed using UHPLC-Q-Exactive and GC-MS/MS techniques under cold storage, at room temperature, and at different cold storage durations. A total of 372 substances were identified, including sugars, amino acids, and flavonoids, among others. Among these substances, 27 were identified as significant biomarkers affecting pear quality. An accurate quantitative method for amino acids was established to systematically verify the change trend of L-aspartic amide, glycine, glutamic acid, and other amino acids. The results indicated that after 30 days of storage at 0 °C, there was a relatively small change in the quality and substance content of 'crown' pears with little effect on their nutritional value. These findings could serve as a reference for optimizing the refrigeration time and temperature for pear fruit.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lyophilizing SERS biosensors to enable translation into an easy-to-use assay.","authors":"Lutfun Naher, Steven M Quarin, Der Vang, Pietro Strobbia","doi":"10.1039/d4ay01667j","DOIUrl":"https://doi.org/10.1039/d4ay01667j","url":null,"abstract":"<p><p>The COVID-19 pandemic has highlighted the importance of point-of-care (POC) pathogen detection. Accurate and accessible diagnostic techniques for virus identification are crucial for controlling the spread of diseases and have profound implications for our communities and global health. Reagentless surface-enhanced Raman scattering (SERS) sensors offer a promising solution for POC testing due to their capability to function without complex processing steps. However, their application in this space is limited by the fact that these solution-based assays are challenging to administer, transport and store. To overcome these limitations, we employed a freeze-drying (lyophilization) process on reagentless SERS sensors and investigated their improved stability and shelf-life. We explored this mechanism using different concentrations of cryoprotectants. Lyophilized sensors were then tested in a mix-and-detect fashion by adding to the dry sensors a drop of the sample, consisting of saliva spiked with target DNA oligonucleotides relative to different SARS-CoV-2 variants. In addition, we further uncovered how lyophilization benefits sensors with a DNA-catalysis mechanism. In summary, our findings indicate that lyophilization substantially enhances the practicality and usability of reagentless SERS sensors, contributing to the translation of this powerful diagnostic tool to POC testing in remote areas with limited resources.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An ultra-sensitive photoelectrochemical biosensing platform based on the AgVO₃/BiOI heterojunction for an enzyme activity inhibition reaction.","authors":"Dangqin Jin, Jiali Xiao, Hui Zhou, Qin Gao, Aiqin Gong, Qin Xu, Yun Shu","doi":"10.1039/d4ay01615g","DOIUrl":"https://doi.org/10.1039/d4ay01615g","url":null,"abstract":"<p><p>An ultra-sensitive photoelectrochemical sensing platform for profenofos detection based on the inhibition of catalase activity was prepared in this work. First, the novel functional nanocomposite material AgVO₃/BiOI was prepared by a hot solvent method and successive ion layer adsorption reaction. Then, chitosan was employed as a dispersion medium to disperse and immobilize the catalase. Under visible light irradiation, the prepared CAT/CS/AgVO₃/BiOI/ITO nanocomposite electrode generates a stable low photocurrent in hydrogen peroxide solution. When this electrode is immersed in a phosphate buffer solution containing profenofos, the activity of the catalase is inhibited, resulting in an increase in the photocurrent. Under the optimized experimental conditions, the profenofos concentration and increase in photocurrent are linearly related in the concentration range of 1 × 10^-10 to 5.0 × 10^-8 mol L^-1, and the limit of detection is 1.0 × 10^-11 mol L^-1. This biosensor shows good selectivity for detecting profenofos in fruits and vegetables, and the determination results of profenofos are satisfactory.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exhaled breath of children swimmers conveniently collected in Teflon bags and used for trihalomethane determination by SPME-GC-MS.","authors":"Shunan Wang, Run Zhou, Lingling Xu, Yamei Hao, Xi Zhang, Limei Chen, Xun Zhu, Pengfei Zhu, Xinliang Ding","doi":"10.1039/d4ay01499e","DOIUrl":"https://doi.org/10.1039/d4ay01499e","url":null,"abstract":"<p><p>Trihalomethanes (THMs) are the most common disinfection by-products in swimming pools; however, they exhibit strong cytotoxicity and genotoxicity, posing health risks. Children are more vulnerable to swimming-related health risks than adults; therefore, a rapid and accurate assessment of internal THM exposure in children swimmers is important for health risk assessment. For internal exposure measurement, collecting exhaled breath samples is more convenient, non-invasive, and easier to perform than collecting blood and urine. Therefore, this study aimed to develop a rapid, accurate, and reproducible method for determining THMs in children swimmers' exhaled breath using solid-phase microextraction (SPME)-gas chromatography-mass spectrometry (GC-MS). The factors influencing the pretreatment procedure, including selecting SPME fibers, extraction temperature, and time, were systematically evaluated. Under the optimized conditions, the instrumental linearity range was 1-200 ng L^-1 with correlation coefficients >0.998. The limit of detection for this method was 0.3-0.5 ng L^-1. The recovery values ranged between 76.87 and 111.49%. Detecting THMs at three different calibration levels using this method had an intra-day precision of 1.31-5.07%, while the inter-day precision was 1.59-11.10% (<i>n</i> = 6). Additionally, the SPME-GC-MS method was used to detect the concentration of THMs in children swimmers' exhaled breath before and after swimming. Trichloromethane was the most abundant THM in the air around the pool and children's exhaled breath, and THM concentrations in the children swimmers' exhaled breath increased significantly after swimming. This study found no significant differences in the concentrations of THMs in the children swimmers' exhaled breath with different swimming durations or frequencies, which may be a result of the unrepresentative sample population and small sample size, and more in-depth and comprehensive studies are needed to verify this conclusion.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method development and optimization for dispersive liquid-liquid microextraction factors using the response surface methodology with desirability function for the ultra-high performance liquid chromatography quadrupole time of flight mass spectrometry determination of organic contaminants in water samples: risk and greenness assessment.","authors":"Tlou Auguston Makwakwa, Elsie Dineo Moema, Titus Alfred Makudali Msagati","doi":"10.1039/d4ay01462f","DOIUrl":"https://doi.org/10.1039/d4ay01462f","url":null,"abstract":"<p><p>A simple, cost effective, and efficient dispersive liquid-liquid microextraction method was developed and optimized for the determination of organic contaminants in different environmental water matrices followed by UHPLC-QTOF-MS analysis. In the preliminary experiments, the univariate optimization approach was used to select tetrachloroethylene and acetonitrile as extraction and disperser solvents, respectively. The significant factors influencing DLLME were screened using full factorial design, and the optimal values for each variable were then derived through further optimization using central composite design with desirability function. The optimal conditions were achieved with 195 μL of tetrachloroethylene as the extraction solvent, 1439 μL of acetonitrile as the disperser solvent, and a sample pH of 5.8. Under these conditions, the method provided detection limits ranging from 0.11-0.48 μg L^-1 and recoveries ranging from 23.32-145.43% across all samples. The enrichment factors obtained ranged from 11.66-72.72. The proposed method was then successfully applied in real water samples. Only benzophenone was detected in the concentration range of 0.79-0.88 μg L^-1 across all the water samples. The calculated risk quotient resulting from benzophenone exposure in water samples showed a low potential risk to human health and the aquatic ecosystem. The method was also evaluated for its environmental friendliness using various metrics tools such as Analytical Eco-Scale (AES), Green Analytical Procedure Index (GAPI), Analytical GREEnness (AGREE), Analytical Greenness for Sample Preparation (AGREEprep), and Sample Preparation Metric of Sustainability (SPMS). Only AES qualified the method as green while it was considered acceptable and sustainable when assessed using SPMS.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of anticoagulant active components in Xuefu Zhuyu decoction <i>via</i> affinity-ultrafiltration coupled with mass spectrometry.","authors":"Chencun Huang, Jie Dai, Xiangchang He, Shiyi Huang, Hongqing Zhao, Weihe He, Guangming Xu","doi":"10.1039/d4ay01162g","DOIUrl":"https://doi.org/10.1039/d4ay01162g","url":null,"abstract":"<p><p>This study aimed to establish an effective affinity-ultrafiltration coupled with mass spectrometry (AUF-MS) method to rapidly screen the active components of antithrombin in Xuefu Zhuyu decoction (XFZYD). The HPLC fingerprint of different batches of XFZYD was established to verify the stability of the compound ingredients. AUF-MS was established for the rapid screening of the active ingredients in XFZYD. Eight active ingredients were screened using AUF-MS: oxypaeoniflorin, chlorogenic acid, amygdalin, isoliquiritin, neohesperidin, hesperidin, glycyrrhizic acid and chikusetsu saponin IVa. Simultaneously, the anticoagulant effect of the active ingredients was verified by performing <i>in vivo</i> and <i>in vitro</i> experiments. The results of this study indicate that AUF-MS can be used for high-throughput screening of active components of traditional Chinese medicine compounds, thus providing a basis for the discovery of thrombin inhibitors and promoting the research progress of antithrombotic drugs. The compounds screened from XFZYD showed an inhibitory effect against thrombin and a certain antithrombotic effect.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nitrogen-doped carbon nanosheet/poly(amidoamine) dendrimer-based electrochemical sensor for nicotine in flavored hookah pipe tobacco.","authors":"Sesethu Makaluza, Nyasha Midzi, Foluke O G Olorundare, Dimpo S Sipuka, Tsholofelo I Sebokolodi, Duduzile Nkosi, Omotayo A Arotiba","doi":"10.1039/d4ay01257g","DOIUrl":"https://doi.org/10.1039/d4ay01257g","url":null,"abstract":"<p><p>Towards the nicotine addiction challenge in the smoking of hookah pipe products, we hereby present the development of an electrochemical sensor for nicotine detection. A nitrogen-doped carbon nanosheet (N-CNS)/poly(amidoamine) dendrimer (PAMAM) nanocomposite-modified electrode was prepared as a sensor for the detection of nicotine in analytical and real samples. The N-CNSs were prepared by a hydrothermal method and dropcast on a glassy carbon electrode followed by electro-deposition of the PAMAM dendrimer to form the sensor (GCE/N-CNSs/PAMAM). The N-CNSs were characterized with electron microscopy, Raman spectroscopy and FTIR. The sensor was characterized with voltammetry and electrochemical impedance spectroscopy. The N-CNS/PAMAM enhanced the electrochemical performance of the electrode towards the oxidation of nicotine. The sensor achieved a detection limit of 0.05 μM in a linear concentration range of 1.93-61.64 μM nicotine standard samples. The sensor showed good reproducibility, repeatability, and selectivity. The sensor was successful in selectively detecting nicotine in two local brands of hookah pipe tobacco with a 113-121 percent recovery. Nicotine, up to a concentration of 0.35-0.39 mg g^-1, was found in the sampled hookah pipe tobacco products suggesting possible harm to human health.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser-induced graphene/gold nanoparticle hybrid sensor for enhanced electrochemical detection of paracetamol.","authors":"Wenbang Yu, Xiaotian Xu, Tian Cao, Zihan Wei, Jing Tang, Min Zhang","doi":"10.1039/d4ay01627k","DOIUrl":"https://doi.org/10.1039/d4ay01627k","url":null,"abstract":"<p><p>This research presents a highly sensitive and selective electrochemical sensor for detecting paracetamol. The sensor is created using laser direct-writing on a flexible PI substrate to form LIG electrodes. Gold nanoparticles (AuNPs) are then synthesized on the working electrode through secondary laser reduction, resulting in an AuNPs/LIG composite. This combination enhances the sensor's electrochemical activity, electron transfer rate, and adsorption capacity. The sensor exhibits a linear response to paracetamol concentrations with a detection limit of 0.086 μM. Testing on Tylenol tablets and tap water showed good recovery rates. The sensor displays strong anti-interference, reproducibility, and stability, making it a promising tool for effective paracetamol monitoring in real-world situations.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of HPV16 utilizing recombinase polymerase amplification with the employment of an extremely low concentration of the probe.","authors":"Ruixiao Zhang, Lin Zhang, Lvping Wu, Shengping You, Rongxin Su, Wei Qi","doi":"10.1039/d4ay01625d","DOIUrl":"https://doi.org/10.1039/d4ay01625d","url":null,"abstract":"<p><p>Human papillomavirus 16 (HPV16) infection is the leading cause of cervical cancer. The current mainstream method for detecting HPV16 is quantitative real-time PCR (qPCR). However, due to its time-consuming nature and reliance on expensive qPCR instruments, there is growing interest in more convenient, rapid and private approaches such as recombinase polymerase amplification (RPA). In this study, we innovated an effective method for HPV16 detection by integrating a RPA system with lateral flow strip (LFS) detection. Primers with optimal efficiency and specificity were designed, and false positive signals caused by dimers were eliminated by reducing the probe concentration. The RPA-LFS method demonstrates high sensitivity and specificity, capable of detecting 230 copies per reaction of HPV16 within 25 min without cross-reactivity to other subtypes. It exhibits good tolerance, remaining unaffected by 1.0% miconazole, 0.5% tioconazole and 1.0% hemoglobin. The results of clinical samples detected by this method were consistent with those of qPCR. The method provides a practical reference for HPV16 diagnosis and can be valuable in home and resource-limited settings, contributing to the reduction of cervical cancer incidence.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}