Analytical Methods最新文献

Detection of blood glucose based on the aggregation induced luminescence effect in 4-formyl-3-hydroxybenzoic acid@ZIF-8 and a ratiometric fluorescence method based on Förster resonance energy transfer between aggregation-induced emission molecules and diallyl phthalate. 基于4-甲酰基-3-羟基苯甲酸acid@ZIF-8聚集致发光效应的血糖检测和基于Förster聚集致发光分子与邻苯二甲酸二烯丙酯共振能量转移的比例荧光法。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-06-18 DOI: 10.1039/d4ay02325k

Xu Xu, Jing Li, Yaodong Zhang

{"title":"Detection of blood glucose based on the aggregation induced luminescence effect in 4-formyl-3-hydroxybenzoic acid@ZIF-8 and a ratiometric fluorescence method based on Förster resonance energy transfer between aggregation-induced emission molecules and diallyl phthalate.","authors":"Xu Xu, Jing Li, Yaodong Zhang","doi":"10.1039/d4ay02325k","DOIUrl":"https://doi.org/10.1039/d4ay02325k","url":null,"abstract":"A novel and simple ratiometric fluorescence detection method for H2O2 and glucose was proposed on the basis of the aggregation-induced luminescence effect of the aggregation-induced emission (AIE) molecule 4-formyl-3-hydroxybenzoic acid (FHBA) in ZIF-8 and the mechanism of fluorescence resonance energy transfer (FRET) between the complex FHBA@ZIF-8 and diallyl phthalate (DAP). First, glucose was oxidized to H2O2 under the catalysis of glucose oxidase. In the presence of horseradish peroxidase, the latter further oxidized o-phenylenediamine to produce yellow fluorescent DAP with an emission wavelength of 560 nm. The fluorescence of FHBA@ZIF-8 at 445 nm can be effectively quenched because the ultraviolet absorption spectrum of DAP overlaps well with the emission spectrum of FHBA@ZIF-8. Therefore, a highly sensitive ratiometric fluorescence detection method for detecting H2O2 and glucose was constructed using the change in the ratio of fluorescence intensity at two wavelengths (F560/F445). The linear range of H2O2 detection by this method is 1-150 μM, and the detection limit is 0.21 μM. The linear range of glucose detection is 1-100 μM, and the detection limit is 0.17 μM. The method was successfully applied for the detection of glucose in human serum samples, and it has broad potential in clinical diagnosis and research.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimisation of electronic nose performance with multi-attention and domain adaptation for fire forensics. 基于多关注和领域适应的火灾取证电子鼻性能优化。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-06-18 DOI: 10.1039/d5ay00474h

Tian-Shu Song, Hui-Rang Hou, Qing-Hao Meng

{"title":"Optimisation of electronic nose performance with multi-attention and domain adaptation for fire forensics.","authors":"Tian-Shu Song, Hui-Rang Hou, Qing-Hao Meng","doi":"10.1039/d5ay00474h","DOIUrl":"https://doi.org/10.1039/d5ay00474h","url":null,"abstract":"The identification of ignitable liquids at fire scenes is a crucial component of forensic investigations. However, conventional analytical methods such as gas chromatography-mass spectrometry, often require costly equipment, specialised expertise, and extended analysis times, which limits their effectiveness for rapid on-site detection. As such, electronic nose (e-nose) technology presents a cost-effective and portable alternative. Nevertheless, inconsistencies in sensor responses across different platforms pose challenges to model transferability which requires independent data collection and model training for each device. This study introduces a multiple attention adversarial transfer learning (MAATL) network aimed at addressing cross-platform variability in ignitable liquid detection using e-noses. The MAATL framework incorporates a multiple attention mechanism to optimise sensor signals, a multi-scale one-dimensional convolutional network for feature extraction, and adversarial learning techniques to enhance domain adaptation. Experimental validation involved five e-nose platforms and four classes of ignitable liquids, namely gasoline, diesel, alcohol, and diluent. The results demonstrated an average classification accuracy of 87% with peak accuracy reaching as high as 97.3%. Furthermore, the Fréchet inception distance (FID) metric indicated significant distribution differences between e-nose platforms, with values ranging from 12.8 to 35.6. Overall, these findings suggest that the proposed method enhances the reliability and scalability of e-nose-based ignitable liquid detection, thereby contributing to more efficient forensic investigations and expanding potential applications in chemical sensing.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen-doped carbon dots for ultrasensitive detection of Hg²⁺ in traditional Chinese medicine and bioimaging applications. 氮掺杂碳点超灵敏检测Hg2+在中药和生物成像中的应用。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-06-17 DOI: 10.1039/d5ay00704f

Yuli Mei, Shuo Yang, Minghui Su, Jianjun Xie, Shengfeng Huang, Zhengjin Jiang, Yang Huang

{"title":"Nitrogen-doped carbon dots for ultrasensitive detection of Hg2+ in traditional Chinese medicine and bioimaging applications.","authors":"Yuli Mei, Shuo Yang, Minghui Su, Jianjun Xie, Shengfeng Huang, Zhengjin Jiang, Yang Huang","doi":"10.1039/d5ay00704f","DOIUrl":"https://doi.org/10.1039/d5ay00704f","url":null,"abstract":"A nitrogen-doped carbon dots (N-CDs)-based fluorescent sensor was developed for ultrasensitive and selective detection of mercury ions (Hg2+) in complex matrices, such as Panax notoginseng (Burk.) F. H. Chen (Sanqi). The synthesized N-CDs exhibited intense blue fluorescence (quantum yield: 35%) with an ultra-small size of 1.93 ± 0.53 nm. Leveraging dynamic quenching mechanisms, the sensor achieved a linear detection range of 0-50 μM for Hg2+ and an exceptionally low limit of detection (LOD) of 0.2825 nM, outperforming existing CD-based sensors. The N-CDs demonstrated remarkable selectivity for Hg2+ in the presence of interfering ions and complex herbal components. Hg2+ in Sanqi samples was quantified by fluorescence analysis (25.5-26.8 μg kg-1, RSD < 2.0%) and cross-validated via Inductively Coupled Plasma Mass Spectrometry (ICP-MS) (24.8-26.2 μg kg-1, RSD < 1.0%), demonstrating N-CDs' reliability for precise Hg2+ detection. Additionally, the N-CDs exhibited excellent biocompatibility and were successfully applied for cellular imaging in A549 cells, showcasing their dual functionality in environmental monitoring and biomedical diagnostics.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A highly sensitive flow injection chemiluminescence method for myoglobin detection in beef samples based on zinc ion-induced "dragging" of heme iron. 基于锌离子诱导血红素铁“拖拽”的高灵敏度流动注射化学发光法检测牛肉样品中的肌红蛋白。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-06-17 DOI: 10.1039/d5ay00774g

Yali Zhang, Yuhan Ma, Yapin Zhao, Jing Ha

引用次数: 0

Ultrasound-enzyme-assisted extraction of flavonols from Polygonatum odoratum using ternary natural deep eutectic solvents. 超声波-酶辅助萃取天然三元深共晶溶剂提取黄酮醇。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-06-17 DOI: 10.1039/d5ay00347d

Yuhan Lou, Jiaying Zhang, Xirui Wu, Jiaxin Li, Qihong Zhou, Wenxiu Li, Luyuan Yang, Bin Guo

{"title":"Ultrasound-enzyme-assisted extraction of flavonols from Polygonatum odoratum using ternary natural deep eutectic solvents.","authors":"Yuhan Lou, Jiaying Zhang, Xirui Wu, Jiaxin Li, Qihong Zhou, Wenxiu Li, Luyuan Yang, Bin Guo","doi":"10.1039/d5ay00347d","DOIUrl":"https://doi.org/10.1039/d5ay00347d","url":null,"abstract":"Natural deep eutectic solvents (NADESs) are emerging green solvents that show great potential in extracting natural products from natural resources. In this study, a third component was introduced into the NADES system, and the screened ternary NADES (choline chloride/fructose/citric acid, 1 : 1 : 2) was combined with ultrasound-enzyme-assisted extraction to extract flavonols from Polygonatum odoratum. The main flavonol components, including quercetin, kaempferol and isorhamnetin, were determined by high-performance liquid chromatography and analyzed in comparison with the extraction efficiency of traditional procedures. The results of Fourier transform infrared spectroscopy and Raman spectroscopy showed that the alteration of intermolecular forces in NADESs promoted the formation of hydrogen bonds between them and flavonols, which significantly increased the extraction yields. After optimization by single-factor and Box-Behnken experiments, the ternary NADES extraction yields could reach 0.24 mg g-1 under the optimal conditions of cellulase dosage 1.1%, ultrasonic time 62 min, ultrasonic temperature 61 °C, and water content 60%. The study provides an efficient and environmentally friendly way to extract flavonols from natural medicinal plants, and also elucidates the potential mechanism of the ultrasound-enzyme-assisted ternary NADESs for the improved extraction efficiency.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence and UV-vis spectrophotometry: a dual-mode competitive approach for selective sensing of dopamine. 荧光和紫外-可见分光光度法：多巴胺选择性传感的双模式竞争方法。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-06-16 DOI: 10.1039/d5ay00787a

Somnath Bali, Arnab Halder, Avijit Mondal

{"title":"Fluorescence and UV-vis spectrophotometry: a dual-mode competitive approach for selective sensing of dopamine.","authors":"Somnath Bali, Arnab Halder, Avijit Mondal","doi":"10.1039/d5ay00787a","DOIUrl":"https://doi.org/10.1039/d5ay00787a","url":null,"abstract":"The quantification of dopamine (DA) is essential due its vital role as a neurotransmitter. Here, we used a sensing strategy, which is based on competition between the DA-resorcinol (RA) reaction and resorcinol-stabilized silver nanoparticle (AgNP) formation in slightly alkaline medium. The AgNP and DA-RA reaction product formation were well characterized, specifically by transmission electron microscopy (TEM) and mass spectrometry, respectively, and also by other well-known techniques. The beauty of this sensing strategy is that the quantification of dopamine using both UV-visible and fluorescence spectrophotometry employing simple chemistry, where the RA molecule itself acts as a reducing agent and stabilizing agent for the formation of AgNPs and also selectively reacts with DA to produce fluorescent entities under alkaline conditions. A dual approach with naked-eye recognition of dopamine with an ambient light source was developed using the well-known chemistry between DA-RA and RA-AgNPs. The interference study was performed using both methods, and almost no interference observed in the fluorescence method. The strategy exhibits high selectivity and sensitivity toward DA with a wide linear range of 0.25 μM to 30 μM and a limit of quantification (LOQ) of 0.25 μM. Additionally, the assay was performed using a human urine sample. The initial assessment of dopamine by the fast colorimetric method, followed by the confirmation with a fluorescence technique, makes this strategy highly promising and thereby attracting much attention for applications.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas12a-assisted electrochemiluminescent detection of ochratoxin A based on COF@Ru coupled with a DNA tetrahedral scaffold. 基于COF@Ru偶联DNA四面体支架的CRISPR/ cas12a辅助电化学发光检测赭曲霉毒素A。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-06-16 DOI: 10.1039/d5ay00427f

Yang Cao, Chun Huang, Kui Li, Xi Zhang, Mengfei Tong, Jiale Tu, Kun Yang, Shuai Yuan, Hong Zhang

{"title":"CRISPR/Cas12a-assisted electrochemiluminescent detection of ochratoxin A based on COF@Ru coupled with a DNA tetrahedral scaffold.","authors":"Yang Cao, Chun Huang, Kui Li, Xi Zhang, Mengfei Tong, Jiale Tu, Kun Yang, Shuai Yuan, Hong Zhang","doi":"10.1039/d5ay00427f","DOIUrl":"https://doi.org/10.1039/d5ay00427f","url":null,"abstract":"To sensitively detect ochratoxin A (OTA), a CRISPR/Cas12a-assisted electrochemiluminescence (ECL) aptasensor based on COF@Ru coupled with a DNA tetrahedral scaffold (DTS) was successfully fabricated. The covalent organic framework (COF) acted as a confined micro-reactor for the tris(2,2'-bipyridyl)ruthenium(II)/tri-n-propylamine (Ru(bpy)32+/TPrA) system and hence, evidently enhanced ECL signals. Au nanoparticles (AuNPs) coupled with DTS caused more ferrocene (Fc)-modified quenching DNA probe molecules to be attached to the surface of the sensing electrode, which could reduce the detection background. Activated CRISPR/Cas12a could effectively amplify the ECL signal since it could cut off a considerable amount of quenching DNA probe molecules and make them move away from the electrode. Owing to these strategies, the fabricated ECL aptasensor could detect OTA as low as 3.5 fg mL-1 in a linear detection range of 10-5-100 ng mL-1. Furthermore, the CRISPR/Cas12a and COF@Ru coupled with DTS-based ECL biosensor possessed high stability and specificity. More importantly, the ECL biosensor could effectively detect OTA in food samples, indicating that it can play a role in monitoring OTA levels in the field of food safety.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triazine-based COF/CNTs as an electrochemical sensor for the simultaneous detection of sunset yellow and tartrazine in food samples. 三嗪基COF/CNTs作为电化学传感器同时检测食品样品中的日落黄和酒黄石。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-06-16 DOI: 10.1039/d5ay00708a

Chih-Ling Yeh, Yu-Jun Ceng, Mu-Xiang Huang, Antonia Trisha Zac R, Bing-Chen Wu, Hui-Ling Lee

引用次数: 0

A review of chemometrics combined with modern spectroscopy technology in fertilizer analysis. 化学计量学与现代光谱学技术在肥料分析中的结合综述。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-06-16 DOI: 10.1039/d4ay02274b

Jixiong Zhang, Wenli Guo, Tongqing Liu, Jingjing Xia

{"title":"A review of chemometrics combined with modern spectroscopy technology in fertilizer analysis.","authors":"Jixiong Zhang, Wenli Guo, Tongqing Liu, Jingjing Xia","doi":"10.1039/d4ay02274b","DOIUrl":"https://doi.org/10.1039/d4ay02274b","url":null,"abstract":"To promote the rapid development of the fertilizer industry, this paper reviewed the research on chemometric methods combined with analytical spectroscopy (including near-infrared spectroscopy, mid-infrared spectroscopy, Raman spectroscopy, laser-induced breakdown spectroscopy, and X-ray fluorescence spectroscopy) in the fertilizer industry over the past two decades. Five parts were described according to the applied spectroscopy. Finally, the paper provides an outlook for the future development of the fertilizer industry. (1) To enhance the overall quality of fertilizers, it is imperative to broaden the scope of critical quality attributes, while simultaneously strengthening the monitoring of the fertilizer production process. (2) The utilization of spectral imaging technology enables the acquisition of both spatial and spectral insights into the ingredients of a fertilizer, providing a comprehensive understanding of its composition. (3) The employment of multi-source spectral data fusion methods has the potential to effectively surmount the inherent constraints of individual spectroscopic techniques, offering a more comprehensive and accurate analysis. (4) The establishment of an open-source spectral database for fertilizers would significantly contribute to the further expansion and advancement of this field. It is anticipated that the integration of chemometrics with analytical spectroscopy will enhance fertilizer production, ultimately fostering a shift towards a more sustainable development path.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the link between polymeric surface composition and emerging bisphenol release in toys and childcare products: insights from the Swiss market. 探索玩具和儿童保育产品中聚合物表面成分与新出现的双酚释放之间的联系：来自瑞士市场的见解。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-06-16 DOI: 10.1039/d5ay00678c

Camille Rime, Sanja Delic, Anne Onidi, Lionel Cretegny, Davide Staedler, Fiorella Lucarini

引用次数: 0