Analytical Methods最新文献

{"title":"A colorimetric-aptamer-based assay for the determination of enrofloxacin through triggering the aggregation of gold nanoparticles.","authors":"Wei-Chuang Kong, Chen-Chen Li, Ai-Hong Zhang, Xin-Long Li, Qian-Rui Gong, Bing-Tan Jin, Xiao-Juan Jia, Xu-Ying Liu, Yan-Fei Kang","doi":"10.1039/d4ay01259c","DOIUrl":"https://doi.org/10.1039/d4ay01259c","url":null,"abstract":"<p><p>Although enrofloxacin (ENR) is a widely used broad-spectrum antibiotic in veterinary medicine, its residues in animals can pose a risk to human health. Thus, we developed a new method for detecting ENR based on aptamers and AuNPs. In the absence of ENR, the aptamers attached to the surface of the AuNPs <i>via</i> electrostatic interactions to protect the AuNPs from NaCl, and the solution remained red. Conversely, the aptamer bonded with ENR, leading the aptamer to detach from the AuNP surface, and the color of the solution changed from red to blue. Based on this principle, ENR can be qualitatively detected by the naked eye and quantitatively detected by measuring the absorbance ratio at 650 nm and 530 nm. The experimental results showed a good linear relationship within the ENR concentration range of 0-400 nM, with a limit of detection (LOD) of 1.72 nM, which is satisfactory for detection in food safety. Additionally, this method has also been successfully applied to the detection of ENR in tap water, river water, milk, serum and urine, with good recovery rates and RSD values of less than 7%, indicating its great potential for ENR detection in environmental water samples. More importantly, the combination of this method with a smartphone platform provided great convenience for on-site and visual detection of ENR, offering promising applicability prospects.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining a Lateral Flow Immunoassay with Triplex Loop-Mediated Isothermal Amplification for the Concurrent Identification of Three Bovine Diarrhea Syndrome Viruses","authors":"Huiyang Xu, Biao Ma, Jiali Li, Yating Song, Jiangbo Shuai, Xiaofeng Zhang, Mingzhou Zhang","doi":"10.1039/d4ay01303d","DOIUrl":"https://doi.org/10.1039/d4ay01303d","url":null,"abstract":"Numerous viruses, such as the bovine rotavirus (BRV), the bovine parvovirus (BPV), and the bovine viral diarrhea virus (BVDV), can cause bovine viral diarrhea syndrome. The global livestock industry has been subjected to significant consequences due to this condition. This results in considerable losses and hinders the production of crucial resources such as meat and milk, which are indispensable for sustaining the world's population. It is crucial to develop a quick and precise way of simultaneously detecting BVDV, BRV, and BPV, as they often occur together in mixed infections. A triplex loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD) assay that can concurrently detect all three viruses was introduced in this study. The amplification process involved 30 minutes of incubation at 65 °C. The limits of detection (LODs) for BVDV, BRV, and BPV were 2.62 × 10^1 copies/μL, 2.43 × 10^1 copies/μL, and 2.50 × 10^1 copies/μL, respectively. The triplex LAMP-LFD assay was further evaluated in 156 anal swab samples, and the results were in agreement with the results of fluorescence quantitative PCR (qPCR) in more than 99% of the cattle. This assay is expected for the quick identification of triplex viruses in the field because it has high sensitivity and specificity and doesn't depend on laboratory equipment or conditions.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142253837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-mode of homogeneous electrochemical-colorimetric biosensing sensor for carcinoembryonic antigen detection based on microfluidic paper-based analysis device","authors":"Yao Zhang, jiaqi xu, Bo Zhang, JiaNing Shen, Tianhao Xue, Xiaoqing Lv, Xiaofang Zhang, Guixian Zhu","doi":"10.1039/d4ay01480d","DOIUrl":"https://doi.org/10.1039/d4ay01480d","url":null,"abstract":"Dual-mode based sensors have drawn a lot of interest due to their high accuracy and sensitivity compared to single-response systems. A simple electrochemical and colorimetric dual-mode sensor based on enzyme-linked immunosorbent assay (ELISA), without complex electrode surface modification, was developed for accurate and sensitive detection of carcinoembryonic antigen (CEA). A target CEA is recognized by an antibody coupled to horseradish peroxidase (HRP), which then oxidizes the substrate 3,3',5,5'-tetramethylbenzidine (TMB) to generate both a colorimetric and an electrochemical signal. A paper-based analysis device (μPAD) with dual-mode homogeneous sensing microfluidic was created, three paper-based detection areas for colorimetric testing, and a two-electrode embedded detection area for electrochemical testing. When applying colorimetric analysis technology, the linear range of CEA detection is 0.6–40 ng/mL, and the limit of detection (LOD) is 0.2 ng/mL. The linear range is 0.1-40 ng/mL and the LOD is 0.03 ng/mL by applying electrochemical analysis. The visibility and intuitiveness of colorimetry provide a reference for higher sensitivity and quick response of the electrochemical method. a smartphone application（APP） was also developed to realize the dual extraction of colorimetric signals. The colorimetric detection system based on ELISA can provide a new path for the development of electrochemical sensing and makes it have inherent self-verification and self-correction functions and is expected to provide more reliable and accurate detection results.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142253839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPRR-Based Diatomic Catalyst Electrochemistry Biosensor for Detecting Cancer-Related Extracellular Vesicles","authors":"Wei Du, Mingxuan Zhou, Guancheng Wang, Mingze Lu, Xiao Wang, Zhirui Guo, Ming Ma, Yu Zhang","doi":"10.1039/d4ay01573h","DOIUrl":"https://doi.org/10.1039/d4ay01573h","url":null,"abstract":"Cancer-associated extracellular vesicles (EVs) are crucial biomarkers for cancer diagnosis due to their rich content of tumor cell information. To efficiently and accurately detect cancer-associated EVs, an electrochemical hydrogen peroxide reduction reaction (HPRR) based biosensor was developed, utilizing enzyme-linked immunosorbent and diatomic catalyst catalytic HPRR current amplification strategies for specific identification and highly sensitive detection. Anti-CXCR4 antibody was immobilized on Au-plated electrode to selectively capture EVs from the sample. Subsequently, Fe/Cu diatomic catalysts, modified with an anti-CD63 antibody, were bounded to the CD63 on the EVs. Quantitative detection of EVs was achieved by measuring the electrical signals from HPRR catalyzed by the labeled Fe/Cu diatomic catalysts. Under optimized conditions, the electrochemical signals exhibited a linear relationship with EV concentration in the range of 500 to 107 particles mL-1, with a detection limit of 117 particles mL-1, maintaining accuracy even in FBS. With its affordability, high sensitivity, and ease of use, this sensor holds significant potential for medication guidance and postoperative evaluation.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142253875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the potential of vancomycin-modified magnetic beads as a tool for sample preparation in diagnostic assays","authors":"Susanne Pahlow, Sabine Schmidt, Tabea Pappert, Lara Thieme, Oliwia Makarewicz, Stefan Monecke, Ralf Ehricht, Karina Weber, Jürgen Popp","doi":"10.1039/d4ay01557f","DOIUrl":"https://doi.org/10.1039/d4ay01557f","url":null,"abstract":"Vancomycin-functionalized micro- or nanoparticles are frequently used for isolation and enrichment of bacteria from various samples. Theoretically, only Gram-positive organisms should adhere to the functionalized surfaces as vancomycin is an antibiotic targeting a peptidoglycan precursor in the cell wall, which in Gram-negative bacteria is shielded by the outer cell membrane. In the literature, however, it is often reported that Gram-negative bacteria also bind efficiently to the vancomycin-modified particles. The goal of our study was to identify the underlying cause for these different findings. For each species several strains, including patient isolates, were investigated, and effects such as day-to-day reproducibility, particle type, and the antimicrobial effect of vancomycin-coupled beads were explored. Overall, we found that there is a strong preference for binding Gram-positive organisms, but the specific yield is heavily influenced by the strain and experimental conditions. For <em>Staphylococcus aureus</em> average yields of approximately 100% were obtained. Respectively, yields of 44% for <em>Staphylococcus cohnii</em>, 22% for <em>Staphylococcus warneri</em>, 17% for <em>Enterococcus faecalis</em> and 5% for vancomycin-sensitive <em>Enterococcus faecium</em> were found<em>.</em> Yields for Gram-negative species (<em>Escherichia coli</em>, <em>Klebsiella pneumoniae</em>, <em>Acinetobacter baumannii</em>) and vancomycin-resistant <em>Enterococcus faecium</em> were below 3%. Our results indicate that the interaction between vancomycin and the <small>D</small>-alanine-<small>D</small>-alanine terminus of the peptidoglycan precursor in the bacterial cell wall is the dominant force responsible for the adherence of the bacteria to the particle surface. It needs to be considered though, that other factors, such as the specific molecules presented on the bacterial surface, as well as the pH, and the ion concentrations in the surrounding medium will also play a role, as these can lead to attractive or repulsive electrostatic forces. Last but not least, when using colony forming unit-based quantification for determining the yields, the influence of cell cluster formation and different sensitivities towards the antimicrobial effect of the vancomycin beads between species and strains needs to be considered.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142253840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Hydrogel-Based Sustained Release System for the Recyclable Uses of Formaldehyde Monitoring in Foods","authors":"Xue Hua Wang, Mei Ju Luo, Xinyu Li, Qingling Yang, Zhishun Guo, Hao Lin Zou, Hong Qun Luo, Nian Bing Li, Bang Lin Li","doi":"10.1039/d4ay01155d","DOIUrl":"https://doi.org/10.1039/d4ay01155d","url":null,"abstract":"4-amino-3-hydramidin-5-mercapto-1, 2, 4-triazacene (AHMT) is pre-doped into agarose hydrogels, and consequently, sustained hydrogel systems with the modulated release performance are constructed, contributing to the point-of-care detections of formaldehyde (FA) with characteristics of simple operation and recyclable uses. With the increases of FA concentrations, the absorbance of those upper-layer solutions responses with linear relationships and the reaction colors gradually increase. The detection of limit is calculated to be 0.013 μg mL-1. In order to verify the practical application, a simple, rapid and low-cost FA detection platform is built on the basis of condition optimizations, and the method has merits of comparable simplicity, high sensitivity and selectivity. More importantly, the developed hydrogels route is recyclable and can be used at least five times without any loss of comparable sensing performance. Significantly, the sensory hydrogels can be employed by the non-skilled people for monitoring food safety of samples, and applied for the practical detection of FA in foods.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142253871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Development on the Extraction, Detection, and Quantification of Vitamin D from Various Sources – An Update","authors":"G. S. Amithabh, M. P. Gireesh Kumar, Kaviya Selvapaniyan, Baskar Baburaj","doi":"10.1039/d4ay01114g","DOIUrl":"https://doi.org/10.1039/d4ay01114g","url":null,"abstract":"Recent advancements in analytical methods for vitamin D and its metabolites have substantially enhanced our capacity to precisely determine and quantify these substances in a wide range of sources, such as biological fluids, fungus, natural and fortified foods. This study focuses on the latest advancements in sample preparation procedures, including solid-phase extraction and environmentally friendly extraction methods. These approaches aim to enhance efficiency and minimize the use of solvents. In addition, we explore the growing popularity of chromatographic methods, specifically LC-MS/MS and developing supercritical fluid chromatography (SFC), which provide improved sensitivity, selectivity, and faster analytical times for comprehensive vitamin D profiling. These developments are crucial for overcoming the challenges presented by low concentrations and complex matrices in the investigation of vitamin D.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142253843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium Dichloroisocyanurate Molecularly Imprinted 2D Photonic Crystal Hydrogel Sensor","authors":"Jianwei Xin, Zihui Meng, Yu Qiao, Yuqi Zhang","doi":"10.1039/d4ay01393j","DOIUrl":"https://doi.org/10.1039/d4ay01393j","url":null,"abstract":"Sodium dichloroisocyanurate (SDIC) is an efficient, safe and convenient halogen disinfectant, which is widely used in hospital disinfection, tableware disinfection and swimming pool disinfection. In order to ensure the use effect and safety, especially in clinical use, it is necessary to carry out effective identification. The effective chlorine content is one of the quality standards for chlorine-containing disinfectants, but its complex experimental operation, poor specificity and portability limit its application. In contrast, testing based on molecularly imprinted photonic crystals sensors provide excellent performance. Therefore, it is of great significance to establish a new and simple SDIC recognition method. We prepared a novel molecularly imprinted two-dimensional (2D) photonic crystal hydrogel (MIPH) for sensitive and label-free recognition of SDIC. The response performance of the resultant MIPH sensor was determined by monitoring the particle spacing changes of the polystyrene (PS) 2D photonic crystal embedded in the hydrogel. The particle spacing changes can be acquired by measuring the diameter changes of the Debye diffraction ring of the MIPH sensor. As the concentration of SDIC in solution increased from 1×10-2 to 1.0 mmol/L, the diameter of Debye diffraction ring increased by 6 mm, and the corresponding photonic crystal particle spacing decreased by 56 nm. The particle spacing change of the MIPH sensor was linear to the SCDI concentration in the range of 1×10-2 to 1.0 mmol/L and the limit of detection (S/N=3) was found to be 3×10-3 mmol/L. The constructed hydrogel sensor was successfully used to detect SDIC in sodium dichloroisocyanurate disinfectant powder with recoveries of 96-100% and RSDs of 7.2-7.6%. Our molecularly imprinted SDIC photonic crystal hydrogels provide a universal strategy for designing other clinical disinfectants sensors.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142253870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of concern: Water-soluble pyridine-based colorimetric chemosensor for naked eye detection of silver ions: design, synthesis, spectral and theoretical investigation","authors":"B. Annaraj, M. A. Neelakantan","doi":"10.1039/d4ay90122c","DOIUrl":"https://doi.org/10.1039/d4ay90122c","url":null,"abstract":"Expression of concern for ‘Water-soluble pyridine-based colorimetric chemosensor for naked eye detection of silver ions: design, synthesis, spectral and theoretical investigation’ by B. Annaraj and M. A. Neelakantan, <em>Anal. Methods</em>, 2014, <strong>6</strong>, 9610–9615, https://doi.org/10.1039/C4AY01592D.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142253841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-cost and highly sensitive colorimetric and visual detection of amikacin in milk using melamine functionalized gold nanoparticles","authors":"Sujuan Zhu, Ding Zuo, Qiang Shi, Jiexuan Yuan, Bing Wang","doi":"10.1039/d4ay01342e","DOIUrl":"https://doi.org/10.1039/d4ay01342e","url":null,"abstract":"This study developed a rapid, low-cost and highly sensitive analytical method using melamine functionalized gold nanoparticles (MA-Au NPs) as a colorimetric sensor to detect amikacin in milk. The MA-Au NPs were synthesized using a self-assembly process and analyzed using absorption spectroscopy, transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) spectroscopy. The MA-Au NPs exhibited a distinct surface plasmon resonance (SPR) absorption peak at 520 nm and demonstrated excellent spherical dispersion. The reaction parameters were optimized, with a Au NP concentration of 2.0 × 10<small>⁻⁷</small> mol L<small>⁻¹</small>, MA concentration of 1.0 × 10<small>⁻⁶</small> mol L<small>⁻¹</small>, pH of 4.0, and reaction time of 15 minutes. In the optimization experiment, the effects of unmodified Au NPs and MA-Au NPs as colorimetric sensors for amikacin determination were compared. As a specific amount of amikacin was added to the Au NP solution, the reaction solution showed a visible change from wine red to purple and then to blue gray; a red-shift in the absorption spectrum of the solutions was observed, which could also be analyzed using the <em>A</em><small>₆₄₀</small>/<em>A</em><small>₅₂₀</small> value, and the color changes were recorded using smartphones for visual detection through RGB (red–green–blue) data image analysis, which made the detection easy and fast. As a result, two approaches were devised using visible absorption spectra and smartphones. A linear correlation was established between the <em>A</em><small>₆₄₀</small>/<em>A</em><small>₅₂₀</small> value and the concentration of amikacin in the range of 4.0 to 16.0 × 10<small>⁻⁸</small> mol L<small>⁻¹</small>. The minimal limit of detection (LOD) for absorption spectrum analysis was 3.75 × 10<small>⁻⁹</small> mol L<small>⁻¹</small>, whereas for smartphone analysis, it was 6.0 × 10<small>⁻⁸</small> mol L<small>⁻¹</small>. The recoveries ranged from 103.09% to 104.51%, while the relative standard deviations (RSDs) ranged from 3.76% to 4.25%. The proposed method was used to detect actual milk samples. The results showed that the method is simple, selective, and practical.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142253842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}