Analytical Methods最新文献

FeCu₃-LDH/MoS₂ superlattice-modified carbon paper as an integrated sensor for the sensitive detection of nitrofurantoin in various samples. feecu3 - ldh /MoS2超晶格修饰碳纸作为集成传感器，用于各种样品中呋喃妥英的灵敏检测。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-04 DOI: 10.1039/d5ay00463b

Xueting Wang, Yuehua Li, Boran Tong, Xingwen Wang, Wei Meng, Jing Zhang, Chao Han

{"title":"FeCu3-LDH/MoS2 superlattice-modified carbon paper as an integrated sensor for the sensitive detection of nitrofurantoin in various samples.","authors":"Xueting Wang, Yuehua Li, Boran Tong, Xingwen Wang, Wei Meng, Jing Zhang, Chao Han","doi":"10.1039/d5ay00463b","DOIUrl":"https://doi.org/10.1039/d5ay00463b","url":null,"abstract":"Nitrofurantoin (NFT) is a broad-spectrum antibiotic used for the treatment of humans, poultry and livestock. Improper use and discharge of NFT can cause serious harm to human health and the environment. Electrochemical analysis has been widely employed in antibiotic detection because of its advantages of simple operation, high sensitivity and rapid response. However, few studies have reported the detection of NFT using this method owing to the limited availability of electrode materials. Layered double hydroxides (LDHs) have attracted widespread attention because of their unique structure and chemical properties as well as the advantages of homogeneous dispersion of laminate elements and adjustable chemical composition. However, their application in NFT detection has rarely been reported due to the inherent defects of weak conductivity and poor stability. To better use LDHs and overcome their shortcomings, a series of superlattice FeCu3-LDH/MoS2 materials were successfully designed using stripping and self-assembly strategies. FeCu3-LDH/MoS2 modified carbon paper (CP) was used as an integrated sensor for NFT detection. Benefiting from the structural advantages of the superlattice material and dual-synergistic catalysis between the LDH and MoS2, FeCu3-LDH/MoS2/CP (1 : 1) exhibited highly sensitive NFT detection, with a low detection limit. FeCu3-LDH/MoS2/CP (1 : 1) could also be successfully applied for NFT determination in real samples, such as human serum, lake water and commercialized drugs, with satisfactory recovery. This work not only provides an effective electrochemical detection method for NFT determination, but also broadens the application of LDHs in the electrochemical field and lays a foundation for the controllable preparation of electrochemical sensors based on superlattice materials.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A systematic review of separation/preconcentration and detection techniques for speciation analysis of arsenic and selenium in water. 水中砷和硒的分离/富集及检测技术综述。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-04 DOI: 10.1039/d5ay00626k

Yanping Li, Cuicui Wang, Jianchao Zhao, Xiaoqi Liu, Mingyuan Ma, Ai Gao, Kangrui Sun

{"title":"A systematic review of separation/preconcentration and detection techniques for speciation analysis of arsenic and selenium in water.","authors":"Yanping Li, Cuicui Wang, Jianchao Zhao, Xiaoqi Liu, Mingyuan Ma, Ai Gao, Kangrui Sun","doi":"10.1039/d5ay00626k","DOIUrl":"https://doi.org/10.1039/d5ay00626k","url":null,"abstract":"The speciation of arsenic and selenium in aquatic environments has garnered significant research attention owing to the critical relationship between elemental speciation and both toxicity profiles and bioavailability mechanisms. Over the past two decades, substantial progress has been achieved in developing advanced analytical methodologies for speciation analysis, particularly addressing the challenges posed by arsenic's pronounced toxicity and selenium's dual biological roles. This review systematically examines the separation/preconcentration techniques and detection strategies employed in various aqueous matrices, including riverine systems, lacustrine environments, marine waters, and surface water bodies. A comprehensive evaluation of pretreatment technologies reveals that the matrix complexity and ultra-trace concentration requirements continue to drive methodological innovations, with particular emphasis on solid-phase extraction and chromatographic separation techniques. Analytical advancements are critically assessed through comparative analysis of spectroscopic detection methods (e.g., ICP-MS, AAS, AFS) coupled with separation modalities (e.g., HPLC, IC, MSPE). Critical validation parameters-including detection limits, recovery rates, precision, and enrichment factors-were systematically summarized alongside methodological characteristics such as typical separation media, key devices employed. Studies have shown that although some new pretreatment and detection technologies have emerged, hyphenated techniques still remain the mainstream in speciation analysis research. The review concludes with evidence-based recommendations for method selection guided by sample matrix characteristics and target speciation requirements while highlighting the imperative for continued innovation in achieving species-specific detection at environmentally relevant concentrations.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mediating in situ-generated fluorescence for Au nanocluster-based multi-color ratiometric detection of alkaline phosphatase. 介导基于金纳米团簇的碱性磷酸酶多色比例检测的原位荧光。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-04 DOI: 10.1039/d5ay00835b

Chao-Zhe Zhu, Shuo Tian, Hua-Nong Ting, Mengke Wang, Yufeng Wang

{"title":"Mediating in situ-generated fluorescence for Au nanocluster-based multi-color ratiometric detection of alkaline phosphatase.","authors":"Chao-Zhe Zhu, Shuo Tian, Hua-Nong Ting, Mengke Wang, Yufeng Wang","doi":"10.1039/d5ay00835b","DOIUrl":"https://doi.org/10.1039/d5ay00835b","url":null,"abstract":"The portable, rapid, and accurate detection methods for alkaline phosphatase (ALP) can significantly improve the efficiency and accuracy of medical testing, especially in early disease screening. Herein, a fluorescent detection was constructed for ALP based on the ALP-mediated in situ-generated fluorescent product for the first time, which employed Au nanoclusters (AuNCs) and ALP-mediated in situ generated fluorescent products as probes by applying 4-methylumbelliferyl phosphate (MeUP) as a substrate. ALP can catalyze the hydrolysis of MeUP to generate 4-methylumbelliferon (MeU), which exhibits blue emission centered at 440 nm. Meanwhile, the AuNCs kept their red fluorescence at 630 nm as a reference signal. Accordingly, a ratiometric fluorescent sensing of ALP was proposed with a consecutive fluorescent color change with a detection limit of 0.0025 U L-1. The pronounced fluorescence signal variation served as the impetus for the development of a facile, cost-effective portable hydrogel kit, which facilitates equipment-free, visual, and quantitative analysis of ALP activity. Additionally, this system was employed for detecting ALP inhibitors using sodium metavanadate (Na3VO4) as a model. Notably, this sensing method and hydrogel kit were applied for ALP detection in human serum samples, yielding promising results.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exposing active sites in zeolites with fluoropyridines as NMR probe molecules. 用氟吡啶作为核磁共振探针分子暴露沸石中的活性位点。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-04 DOI: 10.1039/d5ay00507h

Joseph Hurd, Yujie Ma, Paolo Cerreia Vioglio, Run Zou, Dinu Iuga, Sihai Yang, Xiaolei Fan, Daniel Lee

{"title":"Exposing active sites in zeolites with fluoropyridines as NMR probe molecules.","authors":"Joseph Hurd, Yujie Ma, Paolo Cerreia Vioglio, Run Zou, Dinu Iuga, Sihai Yang, Xiaolei Fan, Daniel Lee","doi":"10.1039/d5ay00507h","DOIUrl":"https://doi.org/10.1039/d5ay00507h","url":null,"abstract":"Active sites in zeolites are crucial for catalysis, but their identification remains challenging due to structural complexity. Solid-state NMR, a key tool for studying zeolites, can struggle with directly detecting acidic properties due to the quadrupolar nature of ubiquitous metal dopants. Here, we use fluorinated pyridine as a probe to identify active sites in HY and HZSM-5, two important solid acid catalysts. 19F NMR effectively detects binding environments, while 19F-27Al polarization transfer reveals elusive penta-coordinated AlV Brønsted acid sites and shows that the dominant active sites are distorted framework-associated AlIV and AlV sites with large quadrupolar coupling constants, shedding light on catalytic properties. Low-temperature (100 K) NMR proves essential for capturing these interactions. This work enhances the understanding of zeolite active sites and highlights the broad applicability of fluorinated probe molecules for surface characterization, offering a cost-effective, highly sensitive approach for catalytic studies.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid detection of colorants in black tea using mid- and short-wave near infrared spectroscopy. 中短波近红外光谱快速检测红茶中的着色剂。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-04 DOI: 10.1039/d5ay00480b

Zhengfei Luo, Yunfeng Chai, Guohua Zhao, Dongling Qiao, Fayin Ye, Lin Lei, Jia Chen

{"title":"Rapid detection of colorants in black tea using mid- and short-wave near infrared spectroscopy.","authors":"Zhengfei Luo, Yunfeng Chai, Guohua Zhao, Dongling Qiao, Fayin Ye, Lin Lei, Jia Chen","doi":"10.1039/d5ay00480b","DOIUrl":"https://doi.org/10.1039/d5ay00480b","url":null,"abstract":"This study investigated the feasibility of using mid- and short-wave near-infrared (MS-NIR) spectroscopy for the rapid detection of colorants in black tea. A portable spectrometer was employed to acquire MS-NIR spectra from black tea samples. Support vector machine (SVM) and random forest (RF) models were developed for the discriminative detection of three colorants: tartrazine, sunset yellow, and ponceau 4R. The spectral preprocessing was optimized, and the predictive performance of the models was evaluated using validation data. The results indicated that, owing to the low concentration of colorants in black tea, the MS-NIR-based model was unsuitable for quantitative detection but effective for determining whether colorants were present. Overall, the discriminative capability of the SVM model surpassed that of the RF model. Following spectral preprocessing, the optimal SVM model achieved accuracy, precision, recall, and F1-score values of (97.50%, 96.15%, 100.00%, 0.9804), (95.00%, 96.00%, 96.00%, 0.9600), and (97.50%, 96.15%, 100.00%, 0.9804) for tartrazine, sunset yellow, and ponceau 4R, respectively. These findings demonstrate the feasibility of using MS-NIR for the rapid and discriminative identification of colorants in black tea. In practical applications, discriminative detection can serve as an initial rapid screening tool, followed by more precise quantitative detection methods to determine colorant concentrations.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of pyrolysis component variations in three azo compounds using headspace gas chromatography-mass spectrometry (HS-GC-MS). 顶空气相色谱-质谱联用分析三种偶氮化合物热解组分的变化。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-04 DOI: 10.1039/d5ay00739a

Jinkun Liu, Zhanfang Liu, Jun Zhu, Guannan Zhang, Yajun Li, Zhenwen Sun, Hong Zhou, Zheng Zhou, Xianhe Deng, Dong Han, Yao Liu

{"title":"Analysis of pyrolysis component variations in three azo compounds using headspace gas chromatography-mass spectrometry (HS-GC-MS).","authors":"Jinkun Liu, Zhanfang Liu, Jun Zhu, Guannan Zhang, Yajun Li, Zhenwen Sun, Hong Zhou, Zheng Zhou, Xianhe Deng, Dong Han, Yao Liu","doi":"10.1039/d5ay00739a","DOIUrl":"https://doi.org/10.1039/d5ay00739a","url":null,"abstract":"As industrial explosive hazardous chemicals, 2,2'-azobis(2-methylpropionitrile) (AIBN), 2,2'-azodi(2-methylbutyronitrile) (AMBN) and 2,2'-azobis(2,4-dimethyl)valeronitrile (ABVN) have caused multiple explosion accidents. In this study, headspace gas chromatography-mass spectrometry (HS-GC-MS) was used to analyze the differences in pyrolysis products of these compounds under gradient temperatures from 60 to 150 °C and aerobic conditions, aiming to provide methodological support for microphysical evidence identification in forensic science. The results showed that with the increase of temperature, the chromatographic peak areas and numbers of the three compounds generally increased. Violent reactions occurred at 80-90 °C, and the reactions ended at approximately 150 °C. Through mass spectrometry matching, retention index (RI), and fragment ion verification, 40 pyrolysis products (including nitriles, ketones, etc.) were identified, among which some products appeared specifically in certain temperature intervals. Twelve differential components (VIP ≥ 1, p < 0.05) were screened out by principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), and the Fisher discriminant model constructed based on these components achieved a 100% classification accuracy for unknown samples. This study reveals the temperature dependence and species specificity of the pyrolysis behavior of azo compounds, provides a novel pyrolysis product fingerprint-based analytical method for the forensic identification of trace evidence at explosion scenes, and is expected to improve the traceability accuracy of hazardous chemicals in complex scenarios.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of a novel optical sensor for the determination of cadmium based on the immobilization of β-2-hydroxybenzyl-5-chloro-2-hydroxyazastyrene on an agarose membrane. 基于琼脂糖膜固定β-2-羟基苯基-5-氯-2-羟基氮杂乙烯的新型镉光学传感器的设计。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-04 DOI: 10.1039/d5ay00817d

Muneera Alrasheedi, Ahmed H Alanazi, Amnah Al Zbedy, Alaa M Younis, Alaa S Amin

{"title":"Design of a novel optical sensor for the determination of cadmium based on the immobilization of β-2-hydroxybenzyl-5-chloro-2-hydroxyazastyrene on an agarose membrane.","authors":"Muneera Alrasheedi, Ahmed H Alanazi, Amnah Al Zbedy, Alaa M Younis, Alaa S Amin","doi":"10.1039/d5ay00817d","DOIUrl":"https://doi.org/10.1039/d5ay00817d","url":null,"abstract":"An optode featuring high selectivity was prepared for cadmium detection via chemically binding β-2-hydroxybenzyl-5-chloro-2-hydroxyazastyrene (HCHAS) onto a clear agarose membrane. Colorimetric analysis of complexation between HCHAS and various metal ions at pH 2.75 revealed that the Cd2+ complex exhibited a markedly higher stability constant than others. As a result, HCHAS was applied as a suitable ionophore for fabricating a Cd2+-selective optode, via chemically anchoring it onto a transparent agarose matrix. The immobilization parameters were optimized and the resulting optode represented a conspicuous color transition from yellow to purple as a result of increasing Cd2+ concentrations in a pH 2.75 buffered solution. Detailed investigations were conducted to assess the influence of pH, ionophore loading, ionic strength, stirring conditions, and reaction duration on the optode's performance. A strong linear relationship was observed over the 4.0-140 ng mL-1 concentration range, with R2 values of 0.9882 and 0.9990, respectively. The high sensitivity of the method was demonstrated by the molar absorptivity and Sandell sensitivity, evaluated at 5.11 × 106 L mol-1 cm-1 and 0.002 ng cm-2, respectively. Furthermore, Cd2+ detection was not considerably influenced by the existence of various potentially interfering ions at concentrations 500-fold higher. The fabricated optode was effectively utilized for the quantification of cadmium ions in fortified food, environmental and biological specimens.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting liposomes and an accelerated CRISPR system for selective imaging of miR-21 in cells. 靶向脂质体和加速CRISPR系统用于细胞中miR-21的选择性成像。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-03 DOI: 10.1039/d5ay00619h

Wenyue Zhang, Mengting Wu, Qingqing Zhang, Xibin Chen, Yingshu Guo

引用次数: 0

Facile and highly sensitive nitrate ion detection via an electrochemical sensor based on a poly 1,8-diaminonaphthalene and copper oxide particle film. 通过基于聚1,8-二氨基萘和氧化铜颗粒膜的电化学传感器进行简单和高灵敏度的硝酸盐离子检测。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-03 DOI: 10.1039/d5ay00655d

Saad Benhaiba, Anas El Attar, Ouissal Salhi, Badr Bouljoihel, Charafeddine Jama, Soad Youssefi, Amine Ezzahi, Mama El Rhazi

{"title":"Facile and highly sensitive nitrate ion detection via an electrochemical sensor based on a poly 1,8-diaminonaphthalene and copper oxide particle film.","authors":"Saad Benhaiba, Anas El Attar, Ouissal Salhi, Badr Bouljoihel, Charafeddine Jama, Soad Youssefi, Amine Ezzahi, Mama El Rhazi","doi":"10.1039/d5ay00655d","DOIUrl":"https://doi.org/10.1039/d5ay00655d","url":null,"abstract":"Excessive nitrate accumulation in soil and water has become a critical environmental concern, threatening ecosystems and public health. This drives the need for designing new electrochemical nitrate sensors based on novel electroactive materials. In this work, we synthesized a hybrid film material composed of poly 1,8-diaminonaphthalene and copper oxide particles electrodeposited on the surface of a carbon paste electrode (Cu2O-CuO/poly 1,8-DAN/CPE). The electrodeposition of the polymer was conducted in galvanostatic mode at a positive current, followed by the electrodeposition of copper in potentiostatic mode at a negative potential. The polymeric film obtained through this facile electropolymerization method showed a uniform dispersion of copper particles and could improve the mechanical stability of the fabricated sensors, thereby leading to a low detection limit of nitrate ions. The Cu2O-CuO/poly 1,8-DAN/CPE was prepared in just 3 min in both the galvanostatic and potentiostatic mode. The developed sensor exhibited a good electrochemical performance for nitrate ions, with a linear range from 2 to 200 μM, strong stability, high reproducibility, and a low detection limit of 0.5 μM. This work revealed that the deposition of polymer can be applied as a promising material in new applications, such as water denitrification.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenium and organic- and inorganic-Hg in stir-fried mushrooms. 炒蘑菇中的硒、有机汞和无机汞。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-02 DOI: 10.1039/d5ay00794a

Jerzy Falandysz, Yuanzhong Wang, Małgorzata Rutkowska, Anetta Hanć, Alwyn R Fernandes

引用次数: 0