Analytical Methods最新文献

{"title":"A near-infrared fluorescent probe for detecting Cu²⁺ and its versatile applications.","authors":"Peijun Li, Junjie Yang, Liqiang Yan","doi":"10.1039/d5ay00580a","DOIUrl":"https://doi.org/10.1039/d5ay00580a","url":null,"abstract":"<p><p>Copper ions (Cu²⁺), although essential for biological systems, pose substantial environmental and health risks at elevated concentrations. Therefore, the detection of Cu²⁺ in the environment is of great significance. In order to develop a simple, rapid, convenient and reliable method for Cu²⁺ analysis, a simple fluorescent probe was successfully prepared, which incorporates the dicyanoisophorone fluorophore and the 2-aminonicotinic acid recognition group. This probe demonstrated stable near-infrared emission at 655 nm, large Stokes shift of 235 nm, exceptional selectivity (detection limit: 0.018 μM), and robust anti-interference capabilities across diverse environments. It enabled direct Cu²⁺ detection in real water matrices, food (rice and mung beans), plant tissues (onion roots), and soil samples. By integrating smartphone-based chromatic analysis and portable test strips, this probe demonstrated on-site visual quantification of Cu²⁺ without complex instrumentation. This study provides a cost-effective Cu²⁺ detection solution for environmental, agricultural, and food safety applications, particularly in resource-limited settings.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a NS-CDs/fluorescein ratiometric fluorescent sensor-integrated smartphone platform for rapid and specific detection of Mn(VII) and captopril.","authors":"Chuntong Liu, Haiyan Qi, Jiayu Zhang, Jun Li, Tao Jing, Qiuying Li, Lixin Qiu, Xiaochen Zhu","doi":"10.1039/d5ay00275c","DOIUrl":"https://doi.org/10.1039/d5ay00275c","url":null,"abstract":"<p><p>The construction of a ratiometric fluorescent sensor based on nitrogen-doped carbon dots (NS-CDs) and fluorescein has been reported for the detection of Mn(VII) and captopril. The detection mechanisms for Mn(VII) and captopril are based on the inner filter effect (IFE) and a redox reaction, respectively. The sensor showed good linearity in the detection range of 0.12-100 μM for Mn(VII) and 0.52-300 μM for captopril, with limits of detection (LODs) of 0.12 μM and 0.52 μM, respectively. Furthermore, a smartphone-based platform for the detection of Mn(VII) and captopril was developed, based on the dual-signal response of color and fluorescence intensity. The system displayed linear signal changes with increasing Mn(VII) and captopril concentrations in the ranges of 0.32-100 μM and 0.52-300 μM, with LODs of 0.32 μM and 0.52 μM, respectively. Finally, the ratiometric sensor was validated for the determination of Mn(VII) and captopril in herb and captopril tablet samples, demonstrating its broad applicability and versatility as a portable, sensitive, and specific detector for Mn(VII) and captopril in various sample types. Overall, our ratiometric fluorescent sensor has potential as a portable, on-site detector for Mn(VII) and captopril analysis.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of melatonin simulated metabolism based on electrochemistry coupled online with high performance liquid chromatography-mass spectrometry.","authors":"Yanyan Niu, Lisi Wang, Yuxue Chen, Juan Zhou, Wei Sun","doi":"10.1039/d5ay00110b","DOIUrl":"https://doi.org/10.1039/d5ay00110b","url":null,"abstract":"<p><p>Online electrochemistry coupled with high-performance liquid chromatography-mass spectrometry (EC-HPLC-MS) technology has emerged as a powerful tool for simulating biological metabolism and pollutant degradation research due to its unique complementary advantages. This study focuses on melatonin, an important endogenous neuroendocrine hormone characterized by its indole ring structure, as a model compound to investigate electrochemical metabolic simulation. The metabolic process of melatonin was mimicked through linear sweep or constant-potential electrolysis, with the resulting electrolysis products directly transferred to an MS or HPLC-MS system for real-time separation and detection. Additionally, comparison with metabolites obtained <i>via in vitro</i> cultured liver microsome assays demonstrated substantial similarity in their phase I metabolite profiles. Electrochemically simulated metabolites exhibited markedly enhanced MS ion responses under optimized conditions. In contrast, phase II metabolites derived from electrochemical simulation diverged completely from microsomal metabolites, with both methods producing limited product diversity. The proposed EC-MS and EC-HPLC-MS approaches effectively address key limitations of traditional metabolic studies, including challenges in biological sample acquisition and metabolic process control, thereby offering a robust and reproducible alternative for metabolism research. It provides a promising strategy for further exploration of metabolic mechanisms, which is worthy of further improvement and promotion in pharmaceutical and environmental research.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous determination of voriconazole and its metabolites in human plasma by capillary electrophoresis with cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography.","authors":"Tai-Ling Chiang, Chung-Ping Yu, Chia-Wen Chen, Yu-Chao Lin, Pei-Ming Chu, Yi-Hui Lin","doi":"10.1039/d5ay00552c","DOIUrl":"https://doi.org/10.1039/d5ay00552c","url":null,"abstract":"<p><p>This study presents a highly efficient analytical approach combining cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography with photodiode array detection for the simultaneous determination of voriconazole (VRC) and its key metabolites, voriconazole <i>N</i>-oxide (VNO) and hydroxy-voriconazole (V-OH), in human plasma. The method utilizes a precisely optimized capillary electrophoresis system with the following conditions: an uncoated fused-silica capillary (30 cm, 50 μm I.D.) filled with phosphate buffer (125 mM, pH 2.5) containing 15% (v/v) acetonitrile and 10% (v/v) propanol, followed by a high-conductivity buffer zone (100 mM phosphate, pH 2.5, 8.3 kPa, 99.9 s). After electrokinetic injection (10 kV, 600 s) of cationic analytes, separation was achieved under -20 kV using phosphate buffer (50 mM, pH 2.5) containing 200 mM sodium dodecyl sulfate. Detection was set at 214 nm. This optimized method demonstrated excellent sensitivity, with a detection limit (<i>S</i>/<i>N</i> = 3) of 0.01 μg mL^-1 for all analytes. The sensitivity was increased by 200 times compared to the conventional MEKC method. The calibration curves showed good linearity, with correlation coefficients exceeding 0.9995 over the ranges of 0.1-10.0 μg mL^-1 for VRC, 0.05-5.0 μg mL^-1 for VNO, and 0.05-1.0 μg mL^-1 for V-OH. Precision and accuracy assessments revealed relative standard deviations (RSDs) and relative errors (REs) of less than 7.2% and 5.2%, respectively. This method has been successfully applied to real plasma samples from patients. It can provide more accurate drug exposure information to assist VRC dosage adjustments, thus improving VRC treatment, and can also be applied in VRC pharmacokinetic studies.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bulk-lysis protocols as a sensitive method for investigation of circulating CK19 cells in the peripheral blood of patients with breast cancer by flow cytometry.","authors":"Daniella Serafin Couto Vieira, Laura Otto Walter, Maria Eduarda Cunha da Silva, Lisandra de Oliveira Silva, Heloísa Zorzi Costa, Chandra Chiappin Cardoso, Fernando Carlos de Lander Schmitt, Maria Cláudia Santos-Silva","doi":"10.1039/d5ay00295h","DOIUrl":"https://doi.org/10.1039/d5ay00295h","url":null,"abstract":"<p><p>Invasive Breast Carcinoma (IBC) is a heterogeneous group of tumours with varying responses to treatment. Proteins such as Cytokeratin 19 (CK19) play a crucial role in the prognostic evaluation of patients, demonstrating significant roles in breast tumours and metastatic progression, and are described as biomarkers used for the detection of disseminated cells in lymph nodes or distant organs in clinically metastasis-free patients (cM0). Thus, the objective of this study was to establish a methodology for investigating circulating CK19+ breast cancer cells (CTCs) in peripheral blood to detect the risk of metastasis early. The study included a total of 62 patients who were treated at the University Hospital of Santa Catarina. The patients were divided into two groups based on the radiological diagnosis of BI-RADS®. The control group (<i>n</i> = 15) included patients with BI-RADS® 3 and 4a, whose malignancy risk was equal to or less than 10%. The IBC group (<i>n</i> = 46) comprised patients with BI-RADS® 4b, 4c and 5, whose malignancy risk was greater than 10%. The sample preparation was carried out using the bulk-lysis technique to concentrate the peripheral blood sample. For the evaluation of CTCs, the panel was defined using the expression of CD45, a pan-leukocyte antigen not expressed in mammary cells, along with two markers present only in circulating metastatic cells and not in leukocytes, CK19 and Pan-CK. The samples were acquired using a FACSCanto II flow cytometer, equipped with FACSDiva 8.0 software, until tube drying was complete. Data analysis was conducted using Infinicyt 2.0 software. The results obtained in this study show that out of the total cases of IBC analyzed (<i>n</i> = 47), 6.38% (<i>n</i> = 3) presented CTCs detected by flow cytometry, correlating with the presence of metastases identified by imaging methods (cM1), indicating the efficiency of flow cytometry for the research of neoplastic emboli in peripheral blood. Thus, the development and standardization of flow cytometry tests can offer faster, more sensitive, and precise results, complementing the diagnosis and monitoring of IBC patients.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The fluorescence spectrum combined with a broad learning system to characterize the content of difenoconazole in cabbage.","authors":"Di Wu, Xiaorong Sun, Yuhan Liu, Cuiling Liu, Jingzhu Wu","doi":"10.1039/d5ay00358j","DOIUrl":"https://doi.org/10.1039/d5ay00358j","url":null,"abstract":"<p><p>Pesticide residue detection plays an important role in vegetable quality and food safety. In this work, we propose a method for detecting difenoconazole pesticide residues based on fluorescence spectroscopy technology and machine learning algorithms. First, through the application of three-dimensional fluorescence spectroscopy technology, we determined that the optimal excitation wavelength for difenoconazole is 420 nm. Next, we constructed qualification determination models using the K-nearest neighbors (KNN) algorithm and decision tree algorithm. We then selected the uninformative variable elimination (UVE) method and successive projections algorithm (SPA) as wavelength selection methods. The selected wavelengths were introduced into the broad learning system (BLS) for modeling the prediction of difenoconazole content and compared with traditional partial least squares regression (PLSR) and echo state network (ESN) models. The results indicate that the decision tree algorithm performed exceptionally well in the qualification determination model, achieving an accuracy of 97% in the prediction set. In the content prediction model, the UVE combined with BLS model exhibited excellent performance in predicting difenoconazole content, with a prediction set coefficient of determination (<i>R</i>_p²) of 0.959 and a root mean square error of prediction (RMSEP) of 1.358. This study has successfully demonstrated the feasibility of combining fluorescence spectroscopy technology with the broad learning system, providing a reference for the online monitoring system of pesticide residue content.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supramolecular solvent-based ultrasound-assisted extraction of fangchinoline and tetrandrine from the roots of <i>Stephaniae tetrandrae</i>.","authors":"Yingzhen Wang, Bingyue Cao, Shanshan Shi, Shulin Gao, Dandan Ge","doi":"10.1039/d4ay02259a","DOIUrl":"https://doi.org/10.1039/d4ay02259a","url":null,"abstract":"<p><p>A supramolecular solvent (SUPRAS) based ultrasound-assisted extraction method was developed for the extraction of fangchinoline and tetrandrine from Radix <i>Stephaniae tetrandrae</i>. SUPRASs were prepared in aqueous solutions containing hexafluorobutanol (HFB) and alkanols for the first time. HFB was used as a coacervation agent and density-regulating agent for the SUPRAS preparation. The biosurfactants, namely hexanol, octanol, and decanol were selected as the amphiphiles. The possible mechanism of the new SUPRAS formation was studied. The hydrogen-bond and hydrophobic interactions between HFB and alkanols are the main driving forces for the new SUPRAS formation. The crucial parameters influencing the extraction yields of FAN and TET, such as the type and content of amphiphile, type and content of coacervation agent, volume of SUPRAS, ultrasound time, and extraction temperature were investigated and optimized. HFB-hexanol SUPRAS was found to be the most effective extraction solvent for the extraction of fangchinoline and tetrandrine from <i>Stephaniae tetrandrae</i>. Under the optimized conditions, HFB-hexanol SUPRAS showed extraction yields of 7.11 and 13.89 mg g^-1 for FAN and TET, respectively. The values were higher than those obtained with other extraction methods. The developed method has a great potential for extracting alkaloids from natural plant materials.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and development of a green high performance liquid chromatography method for determination of 8 anti-epileptic drugs and 2 active metabolites in human serum assessed using AGREE, AGREEprep, GAPI and BAGI.","authors":"Xiao-Han Peng, Fan Zhang, Xin-Feng Xia, Kun Wang, Yan-Lin Zhao, Yan Du, Dao-Quan Tang","doi":"10.1039/d5ay00506j","DOIUrl":"https://doi.org/10.1039/d5ay00506j","url":null,"abstract":"<p><p>Pharmacological treatment with antiepileptic drugs (AEDs) remains the primary choice for most patients with epilepsy, while therapeutic drug monitoring is an important strategy to ensure the efficacy and safety of AEDs. The aim of this study was to optimize and develop a green high-performance liquid chromatography (HPLC) method suitable for simultaneous detection of 8 AEDs and 2 active metabolites including primidone (PRM), lamotrigine (LTG), carbamazepine (CBZ) and its active metabolite 10,11-epoxide (CBZE), oxcarbazepine (OXC) and its active metabolite 10,11-dihydro-10-hydroxyl carbamazepine (MHD), phenobarbital (PHB), eslicarbazepine acetate (ESLA), clonazepam (CZP) and diazepam (DZP) in human serum. The results showed that using chlorzoxazone as the internal standard, ethanol as the protein precipitant of serum, a ZORBAX Eclipse Plus-C18 (150 × 4.6 mm, 3.5 μm) column as the stationary phase, and gradient ethanol and 15 mM potassium dihydrogen phosphate water solution as the green alternative mobile phase, good chromatographic separation for 10 target analytes could be obtained. The calibration curves of 10 analytes demonstrated good linearity within the test range (<i>r</i> > 0.996). The intra- and inter-batch precision and accuracy were all less than 15%, while extraction recoveries were in the range of 74.57-90.89% with relative standard deviation values less than 15%. The results of greenness assessment showed that the proposed method was ecofriendly. Finally, the validated method was successfully applied in the analysis of real serum samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Cu₂O/hemin-GO nanozyme with aptamer-enhanced peroxidase-mimic activity for colorimetric detection of kanamycin in milk.","authors":"Yaoting Mou, Lu Han, Yanhao Yin, Jianghua Liu, Yanqing Xu, Yuhang Tian, Yanfang Wu, Dongfei Chen, Yemin Guo, Xia Sun, Falan Li","doi":"10.1039/d5ay00594a","DOIUrl":"https://doi.org/10.1039/d5ay00594a","url":null,"abstract":"<p><p>Kanamycin (KAN) is an aminoglycoside antibiotic employed for the treatment of bacterial infections in livestock and poultry and is widely used as a veterinary drug in animal husbandry. Its overuse poses serious threats to public health and ecosystems; while traditional detection methods cannot meet the requirements of widespread application and rapid detection, developing rapid and reliable technology for KAN detection is essential. In this study, a colorimetric aptasensor was developed to rapidly detect KAN residues, leveraging the enhanced catalytic effect of aptamers on the peroxidase-like activity of the Cu₂O/hemin-graphene oxide (Cu₂O/hemin-GO) nanozyme. In the presence of hydrogen peroxide, the Cu₂O/hemin-GO nanozyme demonstrated excellent peroxidase-like catalytic activity, oxidizing 3,3',5,5'-tetramethylbenzidine (TMB). The adsorption of aptamers on the nanozyme surface increased the negative charge density and significantly enhanced the affinity of the nanozyme for the positively charged chromogenic substrate TMB, boosting the catalytic activity nearly threefold. Upon exposure to KAN, the specific binding of aptamers to KAN reduced their adsorption on the Cu₂O/hemin-GO nanozyme, leading to a decrease in the peroxidase-like activity of the Cu₂O/hemin-GO nanozyme and a corresponding reduction in color change. The aptasensor exhibited excellent sensitivity with a linear detection range from 0.1 pM to 1 μM and a detection limit of 16 fM. The recovery rates for KAN in milk samples ranged from 92.05% to 110.71%. Furthermore, the colorimetric aptasensor demonstrated high selectivity and reproducibility. Overall, the colorimetric approach achieved the economical, simple and sensitive detection of KAN residues, indicating its promising potential in real-world applications. In addition, this sensing method could be applied to other targets by replacing the aptamer, providing a strategy for developing sensors facilitating rapid detection.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A stable Zn-coordination polymer for the quantitative and selective detection of biomarker phenylglyoxylic acid in urine.","authors":"Rongqi Wang, Ruxue Wang, Zeqin Chen, Laiwen Li, Xinyu Zong, Jing Wang, Ziao Zong","doi":"10.1039/d5ay00405e","DOIUrl":"https://doi.org/10.1039/d5ay00405e","url":null,"abstract":"<p><p>Developing stable sensing materials for the selective detection of phenylglyoxylic acid (PGA) in biological samples is highly important for the early diagnosis and treatment of various diseases. In this work, a Zn(II)-based coordination polymer (CP), {[Zn(L)(bibp)]·bibp·2H₂O}_<i>n</i> (1) (H₂L = 2,6-di(4-carboxylphenyl)-4-(4-(triazol-1-ylphenyl))pyridine and bibp = 4,4'-bis(imidazolyl)biphenyl), with good chemical and thermal stabilities was solvothermally synthesized. It showed an excellent fluorescence turn-off response capability for the detection of the styrene biomarker PGA with outstanding sensitivity, selectivity, recoverability and anti-interference ability. Moreover, experimental and DFT calculations suggested that the mechanism of fluorescence quenching could be attributed to the synergistic effect of the internal filter effect (IFE) and photo-induced electron transfer (PET) process between the complex and PGA.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}