Analytical Methods最新文献

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Rapid and accurate detection of huanglongbing in citrus by elasticity testing using a piezoelectric finger.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/d4ay01952k
Pawan Rao, Shu Huang, Cheryl M Armstrong, Joseph Capobianco, YongPing Duan, Wei-Heng Shih, Wan Y Shih
{"title":"Rapid and accurate detection of huanglongbing in citrus by elasticity testing using a piezoelectric finger.","authors":"Pawan Rao, Shu Huang, Cheryl M Armstrong, Joseph Capobianco, YongPing Duan, Wei-Heng Shih, Wan Y Shih","doi":"10.1039/d4ay01952k","DOIUrl":"https://doi.org/10.1039/d4ay01952k","url":null,"abstract":"<p><p>Rapid and sensitive detection of citrus huanglongbing (HLB) is critical for the control of this devastating disease. In this study, we have evaluated using a piezoelectric finger (PEF) with a 0.4 mm probe to measure the elastic modulus of a leaf to detect HLB in four different species of citrus including grapefruit (GFT), pumelo (PUM), lemon (LEM), and Valencia orange (VAL). Diseased citrus leaves were harvested from trees testing positive for the presence of <i>Candidatus</i> Liberibacter asiaticus (Las), the causal agent of HLB, and included both symptomatic leaves, which were blotchy mottle or yellowing and asymptomatic leaves, which did not display outward symptoms. Healthy leaves were harvested from trees testing negative for Las. The results indicated that the PEF elastic modulus test exhibited an overall 94% sensitivity and 90% specificity against the Las status of the trees for all four citrus types combined. Comparative quantitative real-time polymerase chain reaction (qPCR) tests on the same leaves showed an overall 89% sensitivity and 100% specificity against the Las status of the trees. While a Cohen-Kappa coefficient of 0.81 was obtained between the PEF and qPCR predictions, suggesting a \"strong\" agreement between the PEF and qPCR tests, a more detailed examination indicated that PEF was more sensitive overall in detecting the Las positive trees than qPCR, particularly from asymptomatic leaves for which PEF was 96% sensitive <i>versus</i> 78% sensitive by qPCR, indicating the potential of using PEF for early detection of HLB.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A fast, accurate strategy based on LAMP-NALF for detecting human adenovirus serotype 3 in children.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/d4ay01973c
Qi Yun, Wen Hui Xia, Si Fei Ma, Dong Ming Lu, Meng Gu, Ao Shuang Zhu
{"title":"A fast, accurate strategy based on LAMP-NALF for detecting human adenovirus serotype 3 in children.","authors":"Qi Yun, Wen Hui Xia, Si Fei Ma, Dong Ming Lu, Meng Gu, Ao Shuang Zhu","doi":"10.1039/d4ay01973c","DOIUrl":"https://doi.org/10.1039/d4ay01973c","url":null,"abstract":"<p><p><i>Objective</i>: To establish a rapid, convenient and accurate visual detection protocol for human adenovirus serotype 3 in children's respiratory infections based on LAMP-NALF. <i>Methods</i>: Adenovirus serotypes prevalent in the region (Changzhou City, Jiangsu Province, China) were analyzed by assessing the results of tNGS analysis of respiratory pathogens in children. On this basis, multiple sets of LAMP primers were designed for the adenovirus hexon gene as the detection target. Optimal primer combinations were further screened, and the composition of the reagent system and amplification procedures were improved to achieve efficient amplification. To realize an instrument-independent, fast-testing strategy, by combining NALF, which is based on the principle of lateral chromatography, small molecule antigens were modified for primers. Moreover, amplification products were bound to the corresponding antibodies on nucleic acid chromatography test strips for visualization. Finally, a series of tests were performed to validate the sensitivity, specificity, and methodological consistency of the LAMP-NALF assay. <i>Results</i>: Adenovirus infections in children in this region during this period were mainly caused by serotype 3. The optimal amplification temperature for optimal primers of LAMP was 65 °C. The combination of LAMP and NALF could detect femtogram-grade positive plasmid of the hexon gene; besides, there was no cross-reactivity with five other types of common respiratory pathogens in children. The agreement between the LAMP-NALF assay and tNGS/qPCR for detecting the adenovirus in 100 respiratory clinical specimens was 98%. <i>Conclusion</i>: The combination of LAMP and NALF can achieve rapid, convenient and accurate detection of human adenovirus type 3, which can provide a powerful support for the pathogenic diagnosis of adenoviral infections in children, thus contributing to the promotion of the development of point-of-care testing for pathogens.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A portable and ecological paper-based device for glucose monitoring in peripheral blood mononuclear cell lysates.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/d5ay00057b
Grazia Nota, Wanda Cimmino, Sima Singh, Ibrahim A Darwish, Claudia La Rocca, Fortunata Carbone, Giuseppe Matarese, Stefano Cinti
{"title":"A portable and ecological paper-based device for glucose monitoring in peripheral blood mononuclear cell lysates.","authors":"Grazia Nota, Wanda Cimmino, Sima Singh, Ibrahim A Darwish, Claudia La Rocca, Fortunata Carbone, Giuseppe Matarese, Stefano Cinti","doi":"10.1039/d5ay00057b","DOIUrl":"https://doi.org/10.1039/d5ay00057b","url":null,"abstract":"<p><p>The increasing need for point-of-care (POC) testing has prompted a rise in the popularity of affordable biosensors that are eco-friendly, especially paper-based electrochemical sensors. This research introduces a biodegradable paper-based enzymatic biosensor for detecting glucose levels in intricate biological samples, such as cell lysates. This biosensor uses Prussian Blue (PB) as a mediator and glucose oxidase to detect glucose with excellent accuracy using direct electrochemical signals. Screen printing using Whatman filter paper produced a better biosensor than other substrates. The PB concentration of 12.5 mmol L<sup>-1</sup> was found to be optimal and resulted in an operating potential of -0.1 V, which helped decrease interference from other active substances and improved its selectivity. Calibration was found to be linear up to a concentration of 2 mmol L<sup>-1</sup> with a detection limit of 40 μmol L<sup>-1</sup> and a limit of quantification of 120 μmol L<sup>-1</sup>. Moreover, experiments performed on cell lysates obtained from peripheral blood mononuclear cells (PBMCs) suggest the possible application of biosensors to measure glucose levels <i>in vitro</i> in both stimulated and unstimulated cells. This feature underscores its promise for use in monitoring metabolism and conducting diagnostic applications. The paper-based biosensor is an alternative to the current platform for the development of an eco-friendly, portable glucose-sensitive biosensor for point-of-care monitoring of glucose. Its flexibility and efficiency make it a strong candidate for use in the field of POC diagnostics, especially in areas of limited resources and in conditions where there is a problem with glucose dysregulation including diabetes and other related metabolic disorders.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Strategies for the detection of site-specific DNA methylation and its application, opportunities and challenges in the field of electrochemical biosensors.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/d5ay90024g
Chenliu Ye, Zhibin Zhao, Penghui Lai, Chunmei Chen, Fumei Jian, Haiying Liang, Qiongying Guo
{"title":"Correction: Strategies for the detection of site-specific DNA methylation and its application, opportunities and challenges in the field of electrochemical biosensors.","authors":"Chenliu Ye, Zhibin Zhao, Penghui Lai, Chunmei Chen, Fumei Jian, Haiying Liang, Qiongying Guo","doi":"10.1039/d5ay90024g","DOIUrl":"https://doi.org/10.1039/d5ay90024g","url":null,"abstract":"<p><p>Correction for 'Strategies for the detection of site-specific DNA methylation and its application, opportunities and challenges in the field of electrochemical biosensors' by Chenliu Ye <i>et al.</i>, <i>Anal. Methods</i>, 2024, <b>16</b>, 5496-5508, https://doi.org/10.1039/D4AY00779D.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Qualitative and quantitative analysis of metronidazole formulations using a low-cost near infrared transmission spectrophotometer and chemometric tools.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/d4ay02251c
Moussa Yabré, Abdoul Karim Sakira, Moumouni Bandé, Kouka Luc Delma, Isaïe Nyamba, Loconon Y Achille Yemoa, Souleymane Fofana, Touridomon Issa Somé
{"title":"Qualitative and quantitative analysis of metronidazole formulations using a low-cost near infrared transmission spectrophotometer and chemometric tools.","authors":"Moussa Yabré, Abdoul Karim Sakira, Moumouni Bandé, Kouka Luc Delma, Isaïe Nyamba, Loconon Y Achille Yemoa, Souleymane Fofana, Touridomon Issa Somé","doi":"10.1039/d4ay02251c","DOIUrl":"https://doi.org/10.1039/d4ay02251c","url":null,"abstract":"<p><p>For effective drug quality control and detection of falsified and substandard medicines in developing countries, there is an urgent need to develop reliable, rapid, and inexpensive analytical methods. In this work, a methodology combining a low-cost near infrared transmission spectrophotometer with chemometrics and sample analysis in solution has been applied to develop qualitative and quantitative methods to analyze metronidazole in various formulations. To achieve this, calibration and validation solution samples of metronidazole at different concentrations were prepared. The qualitative method consisted of building a model based on a data driven soft independent modeling of class analogy using metronidazole samples at the concentration of 100 mg L<sup>-1</sup> as the target class. The built model allowed a satisfactory identification of metronidazole at the target concentration and a clear discrimination of the excipient mixture and metronidazole samples at other concentration levels and related active pharmaceutical ingredients used to mimic falsified and substandard drugs. The quantitative method was based on a partial least squares regression model allowing the quantification of metronidazole in the concentration range of 50-125 mg L<sup>-1</sup>. The regression model was fully validated according to the accuracy profile methodology. Both qualitative and quantitative methods were successfully employed to analyze various metronidazole tablet samples collected in Burkina Faso. The obtained outcomes underline the feasibility of using these economically accessible portable spectrophotometers as a first line screening tool for drug quality control and detection of falsified and substandard drugs in developing countries.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and validation of a combined QuEChERS and HPLC-MS/MS method for trace analysis of ten diamide insecticides in agricultural products.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-02-19 DOI: 10.1039/d4ay02117g
Fajun Tian, Zhenzhen Zhou, Junfeng Lu, Chengkui Qiao, Caixia Wang, Tao Pang, Linlin Guo, Jun Li, Rongli Pang, Hanzhong Xie
{"title":"Development and validation of a combined QuEChERS and HPLC-MS/MS method for trace analysis of ten diamide insecticides in agricultural products.","authors":"Fajun Tian, Zhenzhen Zhou, Junfeng Lu, Chengkui Qiao, Caixia Wang, Tao Pang, Linlin Guo, Jun Li, Rongli Pang, Hanzhong Xie","doi":"10.1039/d4ay02117g","DOIUrl":"https://doi.org/10.1039/d4ay02117g","url":null,"abstract":"<p><p>Diamide insecticides are being widely registered worldwide, yet most of them lack established maximum residue limits (MRLs) in agricultural products. In this study, we combined a QuEChERS (quick, easy, cheap, efficient, rugged, and safe) extraction method with high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) analysis to simultaneously identify and quantify ten diamide insecticides in seven matrices for the first time. The method was validated in accordance with SANTE/11312/2021 guidelines, including sensitivity, linearity, trueness, and precision. Excellent linearity (<i>R</i><sup>2</sup> > 0.99) was obtained for all diamide insecticides within the concentration range of 5-1000 µg kg<sup>-1</sup>. The limit of detection (LOD) and limit of quantification (LOQ) were 0.01-1 µg kg<sup>-1</sup> and 5 µg kg<sup>-1</sup>, respectively. The recoveries of the ten diamide insecticides at three levels (5, 100, and 1000 µg kg<sup>-1</sup>) ranged from 76.6% to 108.2% with good intra-day relative standard deviation (RSD<sub>r</sub>) (1.0-13.4%) and inter-day relative standard deviation (RSD<sub>R</sub>) (2.3-15.7%). The proposed method was applied to analyze 70 real agricultural product samples, and only six samples contained diamide insecticides. The results demonstrated that the method was both convenient and reliable for detecting diamide insecticides in agricultural products. The method was then applied to analyze agricultural product samples collected in a field trial to estimate the MRLs for the next step.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retraction: Application of new N- and S-doped amorphous carbon in D-μSPE and its combination with deep eutectic solvent-based DLLME for the extraction of some mycotoxins from soymilk.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-02-19 DOI: 10.1039/d3ay90109b
Anahid Rezaeefar, Mir Ali Farajzadeh, Mahboob Nemati, Mohammad Reza Afshar Mogaddam, Farzaneh Lotfipour
{"title":"Retraction: Application of new N- and S-doped amorphous carbon in D-μSPE and its combination with deep eutectic solvent-based DLLME for the extraction of some mycotoxins from soymilk.","authors":"Anahid Rezaeefar, Mir Ali Farajzadeh, Mahboob Nemati, Mohammad Reza Afshar Mogaddam, Farzaneh Lotfipour","doi":"10.1039/d3ay90109b","DOIUrl":"https://doi.org/10.1039/d3ay90109b","url":null,"abstract":"<p><p>Retraction of 'Application of new N- and S-doped amorphous carbon in D-μSPE and its combination with deep eutectic solvent-based DLLME for the extraction of some mycotoxins from soymilk' by Anahid Rezaeefar <i>et al.</i>, <i>Anal. Methods</i>, 2021, <b>13</b>, 4604-4613, https://doi.org/10.1039/D1AY01057C.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In situ sorbent formation dispersive solid phase extraction for multi-elemental analysis: a new approach using cyclen and a magnetic ionic liquid.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-02-19 DOI: 10.1039/d4ay01111b
Hasanain Najm Abed Altuwaijari, Mohammad Reza Afshar Mogaddam, Mir Ali Farajzadeh, Saeed Mohammad Sorouraddin
{"title":"<i>In situ</i> sorbent formation dispersive solid phase extraction for multi-elemental analysis: a new approach using cyclen and a magnetic ionic liquid.","authors":"Hasanain Najm Abed Altuwaijari, Mohammad Reza Afshar Mogaddam, Mir Ali Farajzadeh, Saeed Mohammad Sorouraddin","doi":"10.1039/d4ay01111b","DOIUrl":"https://doi.org/10.1039/d4ay01111b","url":null,"abstract":"<p><p>This study introduces an <i>in situ</i> sorbent formation dispersive solid-phase extraction method for the simultaneous extraction of seven metal ions (V, Cr, Ni, Cu, Zn, Cd, and Pb) in petroleum samples. For this purpose, a home-made extraction device was used to perform the method on a relatively high volume of sample solution. The method employed cyclen as both a sorbent and a chelator. Also, a magnetic ionic liquid was used as the collection/dissolution solvent. Rigorous optimization yielded a wide linear range, low detection limits, good precision (relative standard deviations ≤8.5%), and high extraction recoveries (56-72%). The method's performance was validated in real petroleum samples from Iraq and Iran, demonstrating its applicability, accuracy, and robustness against matrix effects. The method was successfully utilized for the determination of target metal ions in real samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quality evaluation of Semen Platycladi samples of different origins by internal extractive electrospray ionization mass spectrometry.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-02-19 DOI: 10.1039/d4ay01881h
Ning Wang, Siyu Xie, Peng Liu, Leting Wang, Chao Zhong, Jun Yu, Manman Qin, Huanwen Chen
{"title":"Quality evaluation of Semen Platycladi samples of different origins by internal extractive electrospray ionization mass spectrometry.","authors":"Ning Wang, Siyu Xie, Peng Liu, Leting Wang, Chao Zhong, Jun Yu, Manman Qin, Huanwen Chen","doi":"10.1039/d4ay01881h","DOIUrl":"https://doi.org/10.1039/d4ay01881h","url":null,"abstract":"<p><p>Semen Platycladi is a widely used natural product in Chinese medicine with pharmacological effects such as anti-arteriosclerosis, sleep improvement, antioxidant and anti-inflammatory. The quality of Semen Platycladi is fundamentally important for its therapeutic use. However, the tedious sample pretreatment required by the currently available methods poses great challenge to the high throughput analysis and evaluation of the quality of Semen Platycladi. Here we establish a high-throughput analysis platform for evaluating the quality of various Semen Platycladi samples using Internal Extractive Electrospray Ionization Mass Spectrometry (iEESI-MS). The fatty acids including linoleic acid, linolenic acid, arachidonic acid, stearic acid and palmitic acid, known as one of the major ingredients in Semen Platycladi, were directly quantified in the range of 0.001-100.00 μg mg<sup>-1</sup> (<i>R</i><sup>2</sup> > 0.995) using raw samples of different origins, with limits of detection of 0.02-0.04 μg mg<sup>-1</sup>, limits of quantification of 0.07-0.14 μg mg<sup>-1</sup>, recovery rate of 89.5-102.0%, and relative standard deviation of repeatability (<i>n</i> = 9) of 0.51-3.88%, respectively. The data revealed that these five fatty acids were the main differential compounds, which could be used to differentiate the samples that originated from Hebei, Shandong and Henan provinces. Experimental results demonstrated that iEESI-MS could be a useful platform for fast quality analysis of Semen Platycladi, and thus showing advanced applications in natural product analysis, drug discovery and related fields.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sensitive detection of circulating methylated SEPT9 in hepatocellular carcinoma patients using a novel quantitative PCR assay.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-02-18 DOI: 10.1039/d4ay02168a
Yen Hai Tran, Trang Thuy Dao, Ung Dinh Nguyen, Thien Ba Tran, Loi Phuc Luu, Huy Quang Duong, Tho H Ho
{"title":"Sensitive detection of circulating methylated <i>SEPT9</i> in hepatocellular carcinoma patients using a novel quantitative PCR assay.","authors":"Yen Hai Tran, Trang Thuy Dao, Ung Dinh Nguyen, Thien Ba Tran, Loi Phuc Luu, Huy Quang Duong, Tho H Ho","doi":"10.1039/d4ay02168a","DOIUrl":"https://doi.org/10.1039/d4ay02168a","url":null,"abstract":"<p><p>Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths worldwide. Early detection is crucial, yet reliable biomarkers are limited. Methylated <i>SEPT9</i> (m<i>SEPT9</i>) has emerged as a promising biomarker for HCC. Building upon previous ExBP technology, we enhanced the semi-nested realtime PCR assay by integrating TaqMan probes, enabling quantitative detection of mSEPT9 in plasma samples of HCC patients. The assay was validated using synthetic DNA standards and plasma samples from 49 HCC patients, 20 chronic liver disease (CLD) patients, and 32 healthy donors (HDs). Our assay demonstrated sensitivity in detecting methylation ratios as low as 1 : 100 000. The assay showed a strong linear correlation between Ct values and methylation levels over four orders of magnitude (<i>R</i><sup>2</sup> = 0.96178), indicating robust quantification. Clinically, the assay revealed significant differences in ΔCt values between HCC patients (median ΔCt = 19.55) and controls (CLD: 29.32 and HDs: 26.19, <i>p</i> < 0.005). ROC analysis for HCC <i>vs.</i> controls yielded an AUC of 0.729, with 77.55% sensitivity and 59.62% specificity at the optimal cutoff (≤25.98). Notably, the assay identified 72.73% of HCC cases with AFP levels below 20 ng mL<sup>-1</sup>, underscoring its potential in detecting AFP-negative cases. These findings suggest that the novel m<i>SEPT9</i> assay is a sensitive and specific tool for early HCC detection, offering prognostic value for clinical monitoring.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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