Analytical Methods最新文献

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Removal and detection of phenols through an SPE-HPLC method using microporous organic networks as adsorbent. 使用微孔有机网络作为吸附剂,通过 SPE-HPLC 方法去除和检测苯酚。
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-07-12 DOI: 10.1039/d4ay00799a
Azam Ali, Huipeng Sun, Syed Faheem Askari Rizvi, Ding Nana, Haixia Zhang
{"title":"Removal and detection of phenols through an SPE-HPLC method using microporous organic networks as adsorbent.","authors":"Azam Ali, Huipeng Sun, Syed Faheem Askari Rizvi, Ding Nana, Haixia Zhang","doi":"10.1039/d4ay00799a","DOIUrl":"https://doi.org/10.1039/d4ay00799a","url":null,"abstract":"<p><p>The design and development of a facile synthesis approach to construct novel materials for the rapid adsorption and removal of environmental pollutants are of significant interest. In this work, we report the rational design and facile synthesis of magnetic core-shell-based microporous organic networks, Fe<sub>3</sub>O<sub>4</sub>@MON-TBPT-TEB (TTMON, achieved by reacting 2,4,6-tris(<i>p</i>-bromophenyl) triazine and 1,3,5-triethynylbenzene) and Fe<sub>3</sub>O<sub>4</sub>@MON-TBPM-DEBP (TDMON, achieved by reacting tetrakis (4-bromophenyl) methane and 4-4'-diethynylbiphenyl). These MONs possessed excellent dispersity, electrostatic attraction as well as plenty of π-π and hydrophobic interaction sites enabled them to efficiently absorb targeted environmental pollutants. TTMON and TDMON exhibited excellent adsorption capacities of 440 and 510 mg g<sup>-1</sup>, respectively, at 25 °C for 2,4,6-trichlorophenol (TCP). TCP, 2,4-dichlorophenol (DCP), 2-naphthol (2-NT) and 4-nitrophenol (4-NP) from aqueous solution were treated by both MONs, followed by their analysis with high-performance liquid chromatography (HPLC). For TDMON, the proposed SPE-HPLC-UV method showed an LOD of 0.03 μg L<sup>-1</sup>, LOQ of 0.11 μg L<sup>-1</sup>, and a wide linear range of 1-1000 μg L<sup>-1</sup> for TCP. The adsorption kinetics, thermodynamics, isotherms, effect of pH and humic acid (HA), ionic strength, regeneration, and reusability of the MONs were also studied. The results revealed that the novel-designed MONs have potential applications as efficient adsorbents in sample pretreatment.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chemically modified graphitic carbon nitride nanosheets for the selective turn-off fluorescence detection of Al(III) ions in crabs (Brachyura). 化学修饰石墨氮化碳纳米片用于螃蟹(Brachyura)体内铝(III)离子的选择性熄灭荧光检测。
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-07-12 DOI: 10.1039/d4ay00806e
Y G Abou El-Reash, Osama El-Awady, Faisal K Algethami, Fathi S Awad
{"title":"Chemically modified graphitic carbon nitride nanosheets for the selective turn-off fluorescence detection of Al(III) ions in crabs (Brachyura).","authors":"Y G Abou El-Reash, Osama El-Awady, Faisal K Algethami, Fathi S Awad","doi":"10.1039/d4ay00806e","DOIUrl":"https://doi.org/10.1039/d4ay00806e","url":null,"abstract":"<p><p>The selective and sensitive detection of Al(III) is critically important for human health since the level of Al(III) is an indicator of many diseases in humans. Herein, we developed a simple and sensitive fluorescent sensor for the detection of Al(III) in an aqueous solution based on the fluorescence of hydroxyl-functionalized graphitic carbon nitride nanosheets (HO/g-CN). OH/g-CN nanosheets were synthesized <i>via</i> the thermal pyrolysis of 1,3,5-triazine-2,4,6-triamine (as raw material) at 550 °C for 2 hours, followed by thermal alkali treatment at 730 °C for 2 min. The fluorescence of HO/g-CN at 377 nm (at 290 nm excitation) can be quenched by Al(III) effectively and selectively, and the linear relationship between the concentration of Al(III) and fluorescence intensity is in the range of 1.85-14.82 μM with a detection limit of 0.272 μM. The fluorescence turn-off effect of the Al(III) ion on the prepared HO/g-CN nanosheets could be attributed to the presence of oxygen- and nitrogen-containing functional groups on the surface of HO/g-CN that have chelating interactions with Al(III), leading to quenching. The surface functional groups of OH/g-CN were confirmed using different characterization techniques (FTIR, EDX, and XPS). Moreover, the prepared HO/g-CN exhibited remarkable long-term fluorescence stability in water (>30 days) and minimal toxicity. Importantly, a prepared novel fluorescent sensor (HO/g-CN) was successfully applied for the detection and determination of Al(III) in commercially available crab (Brachyura) samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quality and efficiency assessment of five extracellular vesicle isolation methods using resistive pulse sensing strategy 利用电阻脉冲传感策略评估五种细胞外囊泡分离方法的质量和效率
IF 3.1 3区 化学
Analytical Methods Pub Date : 2024-07-10 DOI: 10.1039/d4ay01158a
Min Yang, Jia Guo, Ze Chen, Ying Liu, Zepeng Sun, Xin Pang, Yinghua Peng
{"title":"Quality and efficiency assessment of five extracellular vesicle isolation methods using resistive pulse sensing strategy","authors":"Min Yang, Jia Guo, Ze Chen, Ying Liu, Zepeng Sun, Xin Pang, Yinghua Peng","doi":"10.1039/d4ay01158a","DOIUrl":"https://doi.org/10.1039/d4ay01158a","url":null,"abstract":"Extracellular vesicles (EVs) have attracted great interest due to their great potential in disease diagnosis and therapy. The separation of EVs from complex biofluids with high purity is essential for the accurate analysis of EVs. Despite various methods, there is still no consensus on the best method for high-quality EVs isolation and reliable mass production. Therefore, it is important to offer a standardized method for characterizing the properties (size distribution, particle concentration and purity) of EV preparations from different isolation methods. Herein, we employed a NanoCoulter Counter based on resistive pulse sensing (RPS) strategy that enabled multi-parameter analysis of single EV to compare the quality and efficiency of different EV isolation techniques including traditional differential ultracentrifugation, ultrafiltration, size exclusion chromatography, membrane affinity binding and polymer precipitation. The data revealed that the NanoCoulter Counter based on RPS strategy was reliable and effective for the characterization of EVs. The results suggested that although higher particle concentrations were observed in three commercial isolation kits and ultrafiltration, traditional differential ultracentrifugation possessed the highest purity. In conclusion, our results from NanoCoulter Counter provided reliable evidence for the assessment of different EVs isolation method, which contributed to the development of EV-based disease biomarkers and treatments.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141571792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Portable X-ray fluorescence spectrometry: a cost-effective method for analysing trace metals in deposited dust. 便携式 X 射线荧光光谱仪:分析沉积尘埃中痕量金属的经济有效方法。
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-07-10 DOI: 10.1039/d4ay00368c
Carlos Ibañez-Del Rivero, Cassandra A Wheeler, Kara L Fry, Mark Patrick Taylor
{"title":"Portable X-ray fluorescence spectrometry: a cost-effective method for analysing trace metals in deposited dust.","authors":"Carlos Ibañez-Del Rivero, Cassandra A Wheeler, Kara L Fry, Mark Patrick Taylor","doi":"10.1039/d4ay00368c","DOIUrl":"https://doi.org/10.1039/d4ay00368c","url":null,"abstract":"<p><p>For projects requiring extensive environmental sampling and rapid decision-making to identify trace metal contamination using dust wipes, the cost and time required for wet chemistry analysis can be prohibitive. Under such circumstances there is a need for a suitable screening method that is cost-effective, efficient, and portable. To address this need, this study investigated the utility of portable X-ray fluorescence (pXRF) for the analysis of trace metals in dust wipes. Here, 316 dust wipe samples from three different geographical settings co-located with mining and smelting operations were investigated for their trace metal loadings (μg m<sup>-2</sup>) of arsenic (As), chromium (Cr), copper (Cu), iron (Fe), manganese (Mn), nickel (Ni), lead (Pb), and zinc (Zn) using pXRF. Results collected using pXRF were compared against inductively coupled plasma mass spectrometry (ICP-MS) concentrations using matched dust wipes (<i>n</i> = 87) to assess reproducibility. A subset of dust wipes (<i>n</i> = 4) were subject to different pXRF analytical scenarios (ranging from 1 to 12 pXRF measurements) using a standardised test duration of 30 seconds to identify the most efficient number of tests for analytical precision. Conducting four pXRF tests on a single wipe (total exposure time of 120 seconds) returned comparable results to ICP-MS and was adopted for analysis of all samples. Results from dust wipes analysed with both ICP-MS and pXRF (<i>n</i> = 87) showed moderate to strong Spearman Rho correlations (rs = 0.489-0.956, <i>p</i> < 0.01) and linear regression coefficients of variation demonstrated good agreement between methods (<i>R</i><sup>2</sup> = 0.432-0.989, <i>p</i> < 0.05). Linear regression equations were used to correct pXRF data to the ICP-MS dust wipe data for samples analysed by both approaches, and applied to pXRF data that were not subject to ICP-MS analysis (<i>n</i> = 229). Application of the correction formula resulted in a substantial improvement of pXRF's accuracy and precision, confirming its effectiveness for assessing trace metals in dust wipes.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Portable Label-Free Electrochemical DNA Biosensor for Rapid Detection of Salmonella Typhi 用于快速检测伤寒沙门氏菌的便携式无标签电化学 DNA 生物传感器
IF 3.1 3区 化学
Analytical Methods Pub Date : 2024-07-09 DOI: 10.1039/d4ay00888j
Asrulnizam Abd Manaf, Nor Syafirah Zambry, Mohd Syafiq Syafiq Awang, Hairul Hisham Hamzah, Ahmad Najib Mohamad, Muhammad Fazli Khalid, Khi Khim Beh, Yazmin Bustami, Nurul Fauzani Jamaluddin, Fatimah Ibrahim, Ismail Aziah
{"title":"Portable Label-Free Electrochemical DNA Biosensor for Rapid Detection of Salmonella Typhi","authors":"Asrulnizam Abd Manaf, Nor Syafirah Zambry, Mohd Syafiq Syafiq Awang, Hairul Hisham Hamzah, Ahmad Najib Mohamad, Muhammad Fazli Khalid, Khi Khim Beh, Yazmin Bustami, Nurul Fauzani Jamaluddin, Fatimah Ibrahim, Ismail Aziah","doi":"10.1039/d4ay00888j","DOIUrl":"https://doi.org/10.1039/d4ay00888j","url":null,"abstract":"A highly accurate, rapid, portable, and robust platform for detecting <em>Salmonella enterica</em> serovar Typhi (<em>S.</em> Typhi) is crucial for early-stage diagnosis of typhoid to avert and control the outbreaks of this pathogen that threaten global public health. This study presents a proof-of-concept for our developed label-free electrochemical DNA biosensor system for <em>S</em>. Typhi detection, which employs a printed circuit board gold electrode (PCBGE), integrated with a portable potentiostat reader. Initially, the functionalized DNA biosensor and target detection were characterized using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) methods using a benchtop potentiostat. Interestingly, the newly developed DNA biosensor can identify target single-stranded DNA concentrations ranging from 10 nM to 20 µM, achieving a detection limit of 7.6 nM within a brief 5-minute timeframe. Under optimal detection conditions, the DNA biosensor exhibits remarkable selectivity, capable of distinguishing a single mismatch base pair from the target single-stranded DNA sequence. We then evaluated the feasibility of the developed DNA biosensor system as a diagnostic tool by detecting <em>S</em>. Typhi in 50 clinical samples using a portable potentiostat reader based upon DPV technique. Remarkably, the developed biosensor can distinctly distinguish between positive and negative samples, indicating that the the miniaturised DNA biosensor system is practical for detecting <em>S</em>. Typhi in real biological samples. The developed DNA biosensor device in this work demonstrates to be a promising point-of-care (POC) device for <em>Salmonella</em> detection due to its swift detection time, uncomplicated design, and streamlined workflow detection system.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141571643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Archaeometric characterization of pottery from the Houjiazhai site (ca. 7.3-5.7 ka BP), Anhui Province, China. 中国安徽省侯家寨遗址(约公元前 7.3-5.7 ka)出土陶器的考古计量特征。
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-07-09 DOI: 10.1039/d4ay00473f
Fei Qi, Jinjiao Qin, Guofeng Wei, Xuhang Kan
{"title":"Archaeometric characterization of pottery from the Houjiazhai site (<i>ca.</i> 7.3-5.7 ka BP), Anhui Province, China.","authors":"Fei Qi, Jinjiao Qin, Guofeng Wei, Xuhang Kan","doi":"10.1039/d4ay00473f","DOIUrl":"https://doi.org/10.1039/d4ay00473f","url":null,"abstract":"<p><p>A combination of polarized light microscopy (PLM), X-ray diffraction (XRD), X-ray fluorescence (XRF), neutron activation analysis (NAA) and thermogravimetry (TG) was used on the pottery from the Houjiazhai site in Anhui, China. The analytical data demonstrate that the clay raw material of the Houjiazhai pottery clay was ordinary fusible clay. Phase I pottery consisted of shell-tempered pottery (with a high CaO content), plant-tempered pottery (with a high P<sub>2</sub>O<sub>5</sub> content), and sand-tempered pottery. The pottery body of Houjiazhai was composed of quartz, feldspars, and minor amounts of mica. In addition, the shell-tempered pottery contains calcite. The results of the NAA analysis suggest that the samples from the two phases have the same place of production. The presence of calcite in the mixture indicates that the firing temperature for shell-tempered pottery from Phase I should not exceed 800 °C, while the thermal expansion curve shows that clay pottery from Phase II could reach 900 °C. Houjiazhai people have made progress in temperature control technology and can fire pottery with higher temperatures.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141557466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Probing action potentials of single beating cardiomyocytes using atomic force microscopy 利用原子力显微镜探测单个跳动心肌细胞的动作电位
IF 3.1 3区 化学
Analytical Methods Pub Date : 2024-07-09 DOI: 10.1039/d4ay00929k
Jianjun Dong, Bowei Wang, Guoliang Wang, Siwei Zhang, Xingyue Wang, Rui Wang, Michael James Cardwell Crabbe, Zuobin Wang
{"title":"Probing action potentials of single beating cardiomyocytes using atomic force microscopy","authors":"Jianjun Dong, Bowei Wang, Guoliang Wang, Siwei Zhang, Xingyue Wang, Rui Wang, Michael James Cardwell Crabbe, Zuobin Wang","doi":"10.1039/d4ay00929k","DOIUrl":"https://doi.org/10.1039/d4ay00929k","url":null,"abstract":"This paper presents a method for using atomic force microscopy to probe action potentials of single beating cardiomyocytes at the nanoscale. In this work, the conductive tip of an atomic force microscope (AFM) was used as a nanoelectrode to record the action potentials of self-beating cardiomyocytes in both the non-constant force contact mode and the constant force contact mode. An electrical model of a tip-cell interface was developed and the indentation force effect on the seal of an AFM conductive tip-cell membrane was theoretically analyzed. The force feedback of AFM allowed for the precise control of tip-cell contact, and enabled reliable measurements. The feasibility of simultaneously recording the action potentials and force information during the contraction of the same beating cardiomyocyte was studied. Furthermore, the AFM tip electrode was used to probe the differences of action potentials using different drugs. This method provides a way at the nanoscale for electrophysiological studies on single beating cardiomyocytes, neurons, and ion channels embedded within the cell membrane in relation to disease states, pharmaceutical drug testing and screening.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141571642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Photonic crystal gas sensors based on metal-organic frameworks and polymers. 基于金属有机框架和聚合物的光子晶体气体传感器。
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-07-09 DOI: 10.1039/d4ay00764f
Jianan Wei, Zhihao Yi, Liu Yang, Ling Zhang, Junchao Yang, Molin Qin, Shuya Cao
{"title":"Photonic crystal gas sensors based on metal-organic frameworks and polymers.","authors":"Jianan Wei, Zhihao Yi, Liu Yang, Ling Zhang, Junchao Yang, Molin Qin, Shuya Cao","doi":"10.1039/d4ay00764f","DOIUrl":"https://doi.org/10.1039/d4ay00764f","url":null,"abstract":"<p><p>A photonic crystal (PC) is an optical microstructure with an adjustable dielectric constant. The PC sensor was deemed a powerful tool for gas molecule detection due to its excellent sensitivity, stability, online use and tailorable optical performance. The detection signals are generated by monitoring the changes of the photonic band gap when the sensing behavior occurs. Recently, many efforts have been devoted to improving the PC sensor's detection performance and reducing technical costs by selecting and refining functional materials. In this case, metal-organic frameworks (MOFs) with a large specific surface, tunable structural properties and polymers with unique swelling properties have attracted increasingly attention. In this review, a systematic review of PC gas sensors based on MOFs and polymers was carried out for the first time. Firstly, the optical properties and gas sensing mechanism of PCs were briefly summarized. Secondly, a detailed discussion of the structural properties and rapid preparation methods of distributed Bragg reflectors (DBRs), opals and inverse opals (IOPCs) was presented. Thirdly, the recent advances in MOF, polymer and MOF/polymer-based PC sensors over the past few years were summarized. It should be noted that the sensitivity and selectivity enhancement strategy by appropriate material species selection, organic ligand functionalization, metal-ion doping, diverse functional material arrays, and multi-component compounding were analyzed in detail. Finally, prospects on PC gas sensors are given in terms of preparation methods, material functionalization and future applications.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141557467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular beacon-peptide probe based double recycling amplification for multiplexed detection of serum exosomal microRNAs 基于分子信标-肽探针的双循环扩增技术用于血清外泌体 microRNA 的多重检测
IF 3.1 3区 化学
Analytical Methods Pub Date : 2024-07-09 DOI: 10.1039/d4ay00629a
Liang Liu, Junlong Cai, Kun Yang, Bo Sun, Wei Liu, Yang Li, Hankun Hu
{"title":"Molecular beacon-peptide probe based double recycling amplification for multiplexed detection of serum exosomal microRNAs","authors":"Liang Liu, Junlong Cai, Kun Yang, Bo Sun, Wei Liu, Yang Li, Hankun Hu","doi":"10.1039/d4ay00629a","DOIUrl":"https://doi.org/10.1039/d4ay00629a","url":null,"abstract":"Exosomal microRNAs (exomiRs) have been shown to play crucial roles as biomarkers for early detection and prognosis of cancer. However, simultaneous quantification of multiplex exomiRs is hindered by methods that require additional steps, such as labeling with fluorophores or gel visualization, which are susceptible to various factors. Herein, we developed a mass spectrometry-detectable and target-triggered method for multiplexed exomiR detection using three enzyme-based double recycling amplification in combination with well-designed molecular beacon-peptide (MBP) probes, called molecular beacon-peptide probe-based double recycling amplification (MBPDRA). MBP probes mediated the double recycling amplification reaction and were released as mass-detectable reporter peptides. In particular, the hybridization of the target microRNAs (miRNAs) with the stem-loop of the probe triggers two consecutive processes. The first cycle involved polymerase strand displacement amplification, leading to the production of complementary DNA (Cycle I), and the second cycle encompassed the recycling exonuclease cleavage of the MBP probe (Cycle II). Subsequently, excess probes were removed by interaction with streptavidin beads via biotin-streptavidin binding. The reporter peptides were released using trypsin and subsequently detected by mass spectrometry. Our method enables quantitative detection of multiple exomiRs with a dynamic range from 0.1 fM to 10 pM and a limit of quantification of 0.1 fM. Moreover, the proposed assay was successfully employed for quantification of three exomiRs, exmiR-21, exmiR-191, and exmiR-451a in the sera of patients with pancreatic cancer. Based on these findings, we believe that the MBPDRA assay holds significant promise as a reliable method for quantifying multiple miRNAs in biomedical research and clinical diagnostics.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141571641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrated magneto-plasmonic nanostructures-based immunoassay for galectin-3 detection 基于磁-等离子纳米结构的集成免疫测定法检测加列汀-3
IF 3.1 3区 化学
Analytical Methods Pub Date : 2024-07-08 DOI: 10.1039/d4ay00972j
Sara Fateixa, Ana L. F. Martins, Beatriz Colaço, Maria António, Ana Daniel-da-Silva
{"title":"Integrated magneto-plasmonic nanostructures-based immunoassay for galectin-3 detection","authors":"Sara Fateixa, Ana L. F. Martins, Beatriz Colaço, Maria António, Ana Daniel-da-Silva","doi":"10.1039/d4ay00972j","DOIUrl":"https://doi.org/10.1039/d4ay00972j","url":null,"abstract":"Cardiovascular diseases remain a leading cause of global mortality, highlighting the need for accurate diagnostic tools and the detection of specific cardiac biomarkers. Surface-enhanced Raman scattering (SERS) spectroscopy has proved to be a promising alternative diagnostic tool to detect relevant biomarkers compared to traditional methods. To our knowledge, SERS methodology has never been used to detect galectin-3 (Gal-3), a crucial biomarker for cardiovascular conditions. Our study aimed to develop plasmonic and magneto-plasmonic nanoplatforms for the sensitive immunodetection of Gal-3 using SERS. Spherical gold nanoparticles (AuNPs) were synthesized and functionalized with 11-mercaptoundecanoic acid (MUDA) to enable antibody binding and 4-mercaptobenzoic acid (4MBA) that served as a Raman reporter due to its intense Raman signal. Following bioconjugation with Gal-3 antibody, the AuNPs were employed in the immunodetection of Gal-3 in phosphate-buffer saline (PBS) solution, offering a limit of detection (LOD) of 12.2 ng/mL and a working range up to 120 ng/mL. Furthermore, our SERS-based immunosystem demonstrated selectivity for Gal-3 (40 ng/mL) in the presence of other biomolecules such as α-amylase, bovine serum albumin and human C-reactive protein. As a proof of concept, we developed magneto-plasmonic nanoparticles composed of silica-coated magnetite decorated with the bioconjugated AuNPs aimed at enhancing the uptake and detection of Gal-3 via SERS coupled with Raman imaging. Our findings underscore the potential of SERS-based techniques for the sensitive and specific detection of biomarkers, holding significant implications for improved diagnosis and surveillance of cardiovascular diseases. Future research will focus on further optimizing these nanoplatforms and their translation into clinical settings.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141571645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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