Analytical Methods最新文献

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Development of a hybrid integrated moisture content measurement method based on near-infrared (NIR) spectroscopy 基于近红外(NIR)光谱的混合集成水分测量方法的研制。
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-16 DOI: 10.1039/D5AY00304K
Guoqing Mu, Fulin Zhang, Wenjing Sun, Shuai He, Jingxiang Liu and Chenyu Wang
{"title":"Development of a hybrid integrated moisture content measurement method based on near-infrared (NIR) spectroscopy","authors":"Guoqing Mu, Fulin Zhang, Wenjing Sun, Shuai He, Jingxiang Liu and Chenyu Wang","doi":"10.1039/D5AY00304K","DOIUrl":"10.1039/D5AY00304K","url":null,"abstract":"<p >To measure the moisture content of the product in the fluidized bed drying (FBD) process in real time, a hybrid integrated soft measurement method based on near-infrared (NIR) spectroscopy is proposed in this paper. The training set for the proposed method is developed using historically acquired spectral data paired with their corresponding moisture content measurements. This dataset is subsequently integrated with several widely used machine learning models, including Partial Least Squares (PLS), Support Vector Regression (SVR), and Decision Trees (DT), and further optimized through the Particle Swarm Optimization (PSO) algorithm. By leveraging diverse data perspectives, the method constructs an optimal hybrid integrated model, ensuring robustness and enhanced predictive performance. To determine the optimal parameters for each base learning, a 5-fold cross-validation approach was employed. Spectral knowledge validation showed that the optimized hybrid integration weights by PSO conformed to the characteristic peaks of moisture, demonstrating that the hybrid integration soft measurement model could reflect the physical knowledge. To validate the effectiveness of the proposed method, three sets of comparative experiments were conducted: (1) predictions using standalone PLS, SVR, and DT models; (2) predictions employing Bagging-integrated PLS, SVR, and DT models; and (3) predictions utilizing the hybrid integrated soft sensing method proposed in this study. The experimental results demonstrate that the proposed hybrid integrated soft sensing method achieves superior prediction accuracy compared to the other approaches, underscoring its efficacy for real-time moisture content measurement in the fluidized bed drying process.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 19","pages":" 3985-3993"},"PeriodicalIF":2.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A gold–silver nanostar 2D array composite structure for rapid SERS determination of PAHs 一种用于多环芳烃快速SERS测定的金银纳米星形二维阵列复合结构
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-16 DOI: 10.1039/D5AY00285K
Haibo Xing, Tong Chen, Yong Qian, Qi Huang, Tianze We, Xiaojun Hu, Jian Zhao and Bo Wang
{"title":"A gold–silver nanostar 2D array composite structure for rapid SERS determination of PAHs","authors":"Haibo Xing, Tong Chen, Yong Qian, Qi Huang, Tianze We, Xiaojun Hu, Jian Zhao and Bo Wang","doi":"10.1039/D5AY00285K","DOIUrl":"https://doi.org/10.1039/D5AY00285K","url":null,"abstract":"<p >This study developed a portable multi-ring aromatic hydrocarbon (PAH) detection tool using a dual-metal surface-enhanced Raman scattering (SERS) substrate. Gold–silver nanostars (AuNS@Ag) were synthesized by a secondary seed-mediated growth method and assembled into a two-dimensional array by a liquid-phase interface self-assembly method, uniformly deposited on a silicon wafer. The PAHs in water samples were identified using a portable Raman spectrometer. The SERS detection platform has high sensitivity and stability, and can detect pyrene and phenanthrene with concentrations of 10–8 M and 0.5 × 10<small><sup>−8</sup></small> M, respectively. The stable dual-metal nanomaterial AuNS@Ag 2D array has potential application prospects and can be used for environmental monitoring.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 18","pages":" 3825-3835"},"PeriodicalIF":2.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A photonic crystal-hydrogel sensor for the monitoring of potassium ions in sweat 用于监测汗液中钾离子的光子晶体-水凝胶传感器
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-16 DOI: 10.1039/D5AY00383K
Bing Lin, Irfan Muhammad, Wenxin Zhang, Yifei Wang and Zihui Meng
{"title":"A photonic crystal-hydrogel sensor for the monitoring of potassium ions in sweat","authors":"Bing Lin, Irfan Muhammad, Wenxin Zhang, Yifei Wang and Zihui Meng","doi":"10.1039/D5AY00383K","DOIUrl":"https://doi.org/10.1039/D5AY00383K","url":null,"abstract":"<p >Sweat contains abundant physiological information critical for health monitoring, making wearable sweat sensors increasingly popular in exercise and training surveillance. Most of the existing sensors are designed to measure physical parameters such as heart rate, blood pressure, and respiration, relying on electronic instruments for biochemical analysis of body fluids. In this study, we present a wearable hydrogel band integrated with a photonic crystal array, for visual, non-invasive and <em>in situ</em> analysis of sweat for sports practitioners. The hydrogel sensor, inspired by the biological properties of onions, demonstrates excellent skin adhesion for efficient sweat collection. This innovative design allows for the development of a simple, reliable, and low-cost sensor capable of detecting potassium ion concentrations and pH levels in sweat. Under optimized conditions, the developed sensor can respond to potassium ion concentrations ranging from 1 mM to 150 mM, with a pH response range of 3 to 9 for human sweat. The dynamic cycling tests of potassium ion concentration and pH in sweat demonstrated that this sensor can effectively monitor electrolyte balance and dehydration during outdoor exercise.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3547-3553"},"PeriodicalIF":2.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Binary light-up fluorescent probe based on silver nanoclusters for MicroRNA detection† 用于MicroRNA检测的银纳米簇二元发光荧光探针
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-15 DOI: 10.1039/D5AY00410A
Marina A. Kapitonova, Zakhar V. Reveguk, Polina S. Malova, Kuan Hu and Alexei I. Kononov
{"title":"Binary light-up fluorescent probe based on silver nanoclusters for MicroRNA detection†","authors":"Marina A. Kapitonova, Zakhar V. Reveguk, Polina S. Malova, Kuan Hu and Alexei I. Kononov","doi":"10.1039/D5AY00410A","DOIUrl":"https://doi.org/10.1039/D5AY00410A","url":null,"abstract":"<p >Silver nanoclusters (Ag NCs) are widely applied in the biosensing of metal ions, small organic molecules, nucleic acids, amino acids, and proteins due to their particular fluorescence and chemical properties. Organic matrices such as DNA are usually employed for Ag NC synthesis and stabilization. They make Ag NC/matrix complexes biocompatible and sensitive to the environment. It has recently been shown that Ag NCs based on DNA matrices are capable of self-assembly and rearrangement followed by a change in the fluorescence and absorbance characteristics. These attributes allow the development of sensors with target molecule detection visible even by the naked eye. Here we suggest a simple one-step turn-on highly specific microRNA-210 sensor based on a fluorescent Ag NC. The main feature of the sensor is the smart design of a binary matrix, which provides the appearance of a bright green fluorescence signal only after Ag NCs/DNA-matrix complexes are bonded to the target sequence. The microRNA detection assay requires no additional action because the process proceeds by itself. A comprehensive optimization of the binary probe structure and location was carried out. An approach to detection leading to minimal background signal was defined as follows. The approach involves the preliminary synthesis of non-fluorescent silver clusters using a single strand of the binary matrix containing a 5′-CCCGTTTT-3′ part. It was shown that these “dark” structures can be stored for at least a month before analysis. The fluorescence intensity of the green Ag NCs increases in the presence of the microRNA-210 sequence, and that dependence on the target concentration tends to be linear in the range of 5–500 nM. The sensor demonstrates specificity to the miR-210 sequence, and the LOD (limit of detection) was established as 5 nM in serum samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 18","pages":" 3729-3738"},"PeriodicalIF":2.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Development of a fluorous trapping reagent for rapid detection of electrophilic reactive metabolites 更正:开发用于快速检测亲电反应代谢物的含氟捕获试剂
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-15 DOI: 10.1039/D5AY90040A
Yusuke Akagi, Hiroyuki Yamakoshi and Yoshiharu Iwabuchi
{"title":"Correction: Development of a fluorous trapping reagent for rapid detection of electrophilic reactive metabolites","authors":"Yusuke Akagi, Hiroyuki Yamakoshi and Yoshiharu Iwabuchi","doi":"10.1039/D5AY90040A","DOIUrl":"https://doi.org/10.1039/D5AY90040A","url":null,"abstract":"<p >Correction for ‘Development of a fluorous trapping reagent for rapid detection of electrophilic reactive metabolites’ by Yusuke Akagi <em>et al.</em>, <em>Anal. Methods</em>, 2024, <strong>16</strong>, 3810–3814, https://doi.org/10.1039/D4AY00577E.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3600-3600"},"PeriodicalIF":2.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d5ay90040a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An ultrasensitive D–A fluorescent probe for dual-mode uranyl detection in environmental and biological systems† 一种用于环境和生物系统中铀酰双模检测的超灵敏D-A荧光探针
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-15 DOI: 10.1039/D5AY00379B
Zhen-ni Yi, Jie Xu, Fang Yang, Shen-neng Wang, Yi-peng Xiao, Yao-wen Wang, Di-yun Chen, Min-hua Su and Hui Sun
{"title":"An ultrasensitive D–A fluorescent probe for dual-mode uranyl detection in environmental and biological systems†","authors":"Zhen-ni Yi, Jie Xu, Fang Yang, Shen-neng Wang, Yi-peng Xiao, Yao-wen Wang, Di-yun Chen, Min-hua Su and Hui Sun","doi":"10.1039/D5AY00379B","DOIUrl":"https://doi.org/10.1039/D5AY00379B","url":null,"abstract":"<p >Given the escalating environmental risks posed by radioactive uranium contamination, there is an urgent demand for rapid and precise assessment of uranium levels in environmental and biological matrices. In this study, an ultrasensitive donor–acceptor fluorescent probe, TPA-BH, was developed for on-site rapid detection of uranium in both environmental samples and living systems. Owing to the synergistic effects of aggregation-induced emission (AIE), twisted intramolecular charge transfer (TICT) and specific coordination reactions, the probe achieved exceptional sensing performance: an ultra-low detection limit (0.0417 ppb, 720-fold below the WHO drinking water threshold), ultra-fast response (&lt;1 min), and large Stokes shift (140 nm) with excellent selectivity across wide pH ranges (2–10). The probe demonstrated remarkable anti-interference capability in complex matrices while maintaining low cytotoxicity, enabling its successful application in real water analysis and live-cell imaging. Systematic investigations including photophysical characterization, real sample analysis, and recognition mechanism studies confirm the probe's potential for early warning of uranium pollution and public health risk assessment.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 18","pages":" 3644-3652"},"PeriodicalIF":2.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fabrication of an ultra-sensitive humidity sensor based on polypyrrole flakes/β-AgVO3 nanowires nanocomposite films via in situ photopolymerization 聚吡啶片/β-AgVO3纳米线纳米复合膜原位光聚合制备超灵敏湿度传感器。
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-14 DOI: 10.1039/D5AY00279F
Pi-Guey Su and Ya-Fang Lin
{"title":"Fabrication of an ultra-sensitive humidity sensor based on polypyrrole flakes/β-AgVO3 nanowires nanocomposite films via in situ photopolymerization","authors":"Pi-Guey Su and Ya-Fang Lin","doi":"10.1039/D5AY00279F","DOIUrl":"10.1039/D5AY00279F","url":null,"abstract":"<p >An ultra-sensitive impedance-type humidity sensor was developed through <em>in situ</em> UV-irradiation photopolymerization of a polypyrrole flakes (PPy Fs)/β-AgVO<small><sub>3</sub></small> nanowires (NWs) nanocomposite film on an alumina substrate. The composition, microstructure, and morphology of the PPy Fs/β-AgVO<small><sub>3</sub></small> NWs nanocomposite films were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The influence of varying PPy Fs concentrations on the electrical properties and humidity-sensing performance of PPy Fs/β-AgVO<small><sub>3</sub></small> nanocomposite films was systematically investigated. A PPy Fs/β-AgVO<small><sub>3</sub></small> NWs nanocomposite film composed of 0.5 mL of PPy Fs and 0.1 g of β-AgVO<small><sub>3</sub></small> NWs exhibited a broad operating humidity range, exceptional sensitivity, satisfactory linearity, minimal hysteresis, rapid response/recovery time, and low temperature dependence. The humidity-sensing mechanism of the PPy Fs/β-AgVO<small><sub>3</sub></small> NWs nanocomposite film-based sensor was analyzed using complex impedance spectra.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 19","pages":" 3994-4001"},"PeriodicalIF":2.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A portable paper-based sensor for simultaneous determination of sodium and potassium ions in the human plasma matrix 用于同时测定人血浆基质中钠离子和钾离子的便携式纸基传感器
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-14 DOI: 10.1039/D5AY00234F
Mohammed Alqarni
{"title":"A portable paper-based sensor for simultaneous determination of sodium and potassium ions in the human plasma matrix","authors":"Mohammed Alqarni","doi":"10.1039/D5AY00234F","DOIUrl":"https://doi.org/10.1039/D5AY00234F","url":null,"abstract":"<p >Accurate determination of sodium and potassium ions in biological fluids is crucial for managing various health conditions, as these electrolytes play a vital role in regulating physiological processes and maintaining overall well-being. A low-cost portable paper-based sensor has been developed for the simultaneous determination of sodium and potassium in the human plasma matrix. It comprises three electrodes on a single paper substrate: a solid-state polyvinyl butyral reference electrode, a sodium selective electrode and a potassium selective electrode. The sensor utilizes conductive functionalized multi-walled carbon nanotubes, selective ionophores and dyes to enhance sensing capabilities. It demonstrates high selectivity for sodium and potassium ions, with a linear range of 10<small><sup>−6</sup></small> to 10<small><sup>−2</sup></small> M and a Nernstian response (slopes of 59.08 ± 0.32 and 59.15 ± 0.28 mV per decade for sodium and potassium, respectively). The sensor provides a reproducible stable potential over 180 days and a dynamic response time of 10 s. The described paper-based sensor is an excellent tool for point-of-care analysis of sodium and potassium imbalances due to its simplicity, cost-effectiveness and disposability. It provides fast and reliable determination of sodium and potassium ions in the human plasma matrix, making it a valuable tool for managing their imbalances in clinical settings.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3499-3508"},"PeriodicalIF":2.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sensitive gas spectroscopy method for real-time determination of urease activity via ammonia production† 灵敏气相光谱法实时测定氨生成脲酶活性†
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-14 DOI: 10.1039/D4AY02270J
Letícia Andrade Simões Lopes, Dominik Clara Luz Lopes, Leonardo Mota, Guilherme Rodrigues Lima, Bruno José Rodrigues Alves and Marcelo Gomes da Silva
{"title":"Sensitive gas spectroscopy method for real-time determination of urease activity via ammonia production†","authors":"Letícia Andrade Simões Lopes, Dominik Clara Luz Lopes, Leonardo Mota, Guilherme Rodrigues Lima, Bruno José Rodrigues Alves and Marcelo Gomes da Silva","doi":"10.1039/D4AY02270J","DOIUrl":"https://doi.org/10.1039/D4AY02270J","url":null,"abstract":"<p >In this study we propose the use of a laser-based photoacoustic spectrometer as a new method to investigate the kinetics of ammonia (NH<small><sub>3</sub></small>) emission from the hydrolysis of urea (H<small><sub>2</sub></small>N–CO–NH<small><sub>2</sub></small>) in the presence of urease. Experiments explored the effects of varying the amounts of water and urease on nitrogen (N) loss from urea aqueous solutions. A linear increase in N–NH<small><sub>3</sub></small> loss and the maximum emission rate (<em>r</em><small><sub>NH<small><sub>3</sub></small></sub></small>) was observed with increasing certified urease concentrations. Specifically, the addition of 10 units of urease resulted in an N loss of 8.1%, with a sensitivity of approximately 0.8% per unit of urease, and reached 12.8 μmol min<small><sup>−1</sup></small> with a sensitivity of 1.1 μmol min<small><sup>−1</sup></small> per unit of urease for <em>r</em><small><sub>NH<small><sub>3</sub></small></sub></small>. These relationships enabled the estimation of urease quantities in commercial soy flour extracts. Variations in water and urease proportions revealed that maximum NH<small><sub>3</sub></small> emissions occurred within the first 2–5 h, with the highest N–NH<small><sub>3</sub></small> loss value attaining (9 ± 1)% for samples containing 2.0 mL soy flour extracts and additional water. For urease concentration assays, N–NH<small><sub>3</sub></small> loss and <em>r</em><small><sub>NH<small><sub>3</sub></small></sub></small> were (14.4 ± 0.1)% and (25.3 ± 0.1) μmol min<small><sup>−1</sup></small>, respectively, with 4.0 mL of soy flour extract. The results underscored the dominant influence of urease compared to that of water in urea hydrolysis. The PA spectrometer demonstrated sufficient sensitivity for detecting NH<small><sub>3</sub></small>, rendering it a promising tool for studying urease activity in urea decomposition. Future work could explore this system under crop field conditions to elucidate the roles of urease and water in the cycling of nutrients within agroecosystems.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 17","pages":" 3490-3498"},"PeriodicalIF":2.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nutritional and techno-functional characterization of protein isolates obtained from porcine heart 猪心脏分离蛋白的营养和技术功能特性
IF 2.7 3区 化学
Analytical Methods Pub Date : 2025-04-11 DOI: 10.1039/D5AY00151J
Marielle Maria Oliveira Paula, Ana Paula Rocha de Moura, Bruna Fernandes Andrade, Simone de Fátima Viana da Cunha, Paulo Rogério Fontes, Alcinéia de Lemos Souza Ramos and Eduardo Mendes Ramos
{"title":"Nutritional and techno-functional characterization of protein isolates obtained from porcine heart","authors":"Marielle Maria Oliveira Paula, Ana Paula Rocha de Moura, Bruna Fernandes Andrade, Simone de Fátima Viana da Cunha, Paulo Rogério Fontes, Alcinéia de Lemos Souza Ramos and Eduardo Mendes Ramos","doi":"10.1039/D5AY00151J","DOIUrl":"https://doi.org/10.1039/D5AY00151J","url":null,"abstract":"&lt;p &gt;Given the growing demand for protein sources and the premise of meeting global requirements, there is a need for more efficient use of by-products generated in the slaughter process, like pig heart, which in turn are highly nutritious and also have the capacity for techno-functional application. The pH-shifting technique using aqueous solutions allows the efficient recovery of proteins of high nutritional quality and with improved functional properties, but the muscle protein extraction could be improved using saline solutions that directly influence the yield and final quality of the protein isolate. However, the muscle heme pigments oxidize during extraction, generating a blackish color of the isolates, making their use in foods less attractive. An alternative would be to use nitrite salts in the extraction solution since they interact with heme pigments forming a reddish heat-stable nitrous pigment. Thus, this study aimed at obtaining and characterizing proteins isolated from the porcine heart (HPI), extracted with aqueous (HPI&lt;small&gt;&lt;sub&gt;aq&lt;/sub&gt;&lt;/small&gt;) or saline (HPI&lt;small&gt;&lt;sub&gt;sal&lt;/sub&gt;&lt;/small&gt;) solutions, stabilized or not with sodium nitrite. Treatments did not influence the isolates' protein content (89.0%), and HPI&lt;small&gt;&lt;sub&gt;sal&lt;/sub&gt;&lt;/small&gt; had a higher salt-soluble (53.8 &lt;em&gt;vs.&lt;/em&gt; 47.0%) and lower insoluble (19.2 &lt;em&gt;vs.&lt;/em&gt; 24.1%) proteins content than HPI&lt;small&gt;&lt;sub&gt;aq&lt;/sub&gt;&lt;/small&gt;. The total heme pigments and collagen contents were unaffected by the extraction solution, but they were higher in samples treated with nitrite (921.6 &lt;em&gt;vs.&lt;/em&gt; 707.6 μg hematin per g). Saline extraction only reduces the color intensity (&lt;em&gt;C&lt;/em&gt;* = 9.1 &lt;em&gt;vs.&lt;/em&gt; 12.7), but HPIs extracted with nitrite had a lighter (&lt;em&gt;L&lt;/em&gt;* = 62.4 &lt;em&gt;vs.&lt;/em&gt; 59.2), reddish (&lt;em&gt;h&lt;/em&gt; = 65.7 &lt;em&gt;vs.&lt;/em&gt; 69.5°) and less intense color (&lt;em&gt;C&lt;/em&gt;* = 26.5 &lt;em&gt;vs.&lt;/em&gt; 30.7). The oil retention, water absorption, and emulsifying capacity (EC) of isolates were not affected by the extraction solutions, but the saline extraction reduced the emulsion stability (ES) and the nitrite-based-treatment reduced EC and ES. The isolates contained high levels of iron (165.8 mg kg&lt;small&gt;&lt;sup&gt;−1&lt;/sup&gt;&lt;/small&gt;) and calcium (53.7 mg kg&lt;small&gt;&lt;sup&gt;−1&lt;/sup&gt;&lt;/small&gt;), with higher sodium (85.6 &lt;em&gt;vs.&lt;/em&gt; 8.7 mg g&lt;small&gt;&lt;sup&gt;−1&lt;/sup&gt;&lt;/small&gt;) and heme iron (62.5 &lt;em&gt;vs.&lt;/em&gt; 58.7 mg kg&lt;small&gt;&lt;sup&gt;−1&lt;/sup&gt;&lt;/small&gt;) content in HPI&lt;small&gt;&lt;sub&gt;sal&lt;/sub&gt;&lt;/small&gt; than HPI&lt;small&gt;&lt;sub&gt;aq&lt;/sub&gt;&lt;/small&gt;. All the essential amino acids (EAAs) were found in the isolates, with a higher EAA index (160 &lt;em&gt;vs.&lt;/em&gt; 128) and estimated protein efficiency (51 &lt;em&gt;vs.&lt;/em&gt; 41) in HPI&lt;small&gt;&lt;sub&gt;aq&lt;/sub&gt;&lt;/small&gt; than HPI&lt;small&gt;&lt;sub&gt;sal&lt;/sub&gt;&lt;/small&gt;. The results indicate that the HPI&lt;small&gt;&lt;sub&gt;sal&lt;/sub&gt;&lt;/small&gt; has a high potential to be applied as a techno-functional protein ingredient in meat product formulations, while the HPI&lt;small&gt;&lt;sub&gt;aq&lt;/sub&gt;&lt;/small&gt; has the most interesting profile","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 18","pages":" 3767-3780"},"PeriodicalIF":2.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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