Analytical Methods最新文献_第10页

Insights into the degradation behaviour of tucatinib under stress conditions using liquid chromatography high resolution tandem mass spectrometry. 利用液相色谱-高分辨率串联质谱法研究图卡替尼在应激条件下的降解行为。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-16 DOI: 10.1039/d6ay01576j

Sai Ram Prasad Kollu, Rayeesa Seema, Debasish Swain

{"title":"Insights into the degradation behaviour of tucatinib under stress conditions using liquid chromatography high resolution tandem mass spectrometry.","authors":"Sai Ram Prasad Kollu, Rayeesa Seema, Debasish Swain","doi":"10.1039/d6ay01576j","DOIUrl":"https://doi.org/10.1039/d6ay01576j","url":null,"abstract":"Tucatinib is a kinase inhibitor drug used to treat unresectable or metastatic HER-2 positive breast cancer in combination with Trastuzumab and Capecitabine. Forced degradation studies assess the inherent stability of pharmaceutical compounds, while LC-MS/MS facilitates the identification and characterization of the resulting degradation products. In the present study, the stability of Tucatinib (TCB) was investigated under various stress conditions (hydrolytic, photolytic, thermal, and oxidative conditions). It was demonstrated that TCB was susceptible to hydrolytic (acid, base, and neutral) as well as oxidative degradation, yielding 11 degradation products, of which 9 are reported for the first time. A validated UHPLC method was developed using an Agilent Eclipse Plus C18 (4.6 × 150 mm, 5 µm) column with gradient elution using 0.1% formic acid in water and methanol as mobile phases. Mass characterization of the degradation products was carried out on an Exploris 120 Orbitrap mass spectrometer using the same chromatographic conditions. Also, degradation kinetics was assessed to define the trend followed by TCB under different stress conditions. Thus, the present study effectively presents a detailed degradation profile of TCB, and the developed method finds its use for the routine analysis of TCB and formulation development in bulk and dosage forms.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hairpin competition amplification driven single-CpG-site resolution DNA methylation analysis. 发夹竞争扩增驱动单cpg位点分辨率DNA甲基化分析。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-16 DOI: 10.1039/d6ay01922f

Guodong Zhao, Hui Li, Rui Zhang, Yanmiao Dai

{"title":"Hairpin competition amplification driven single-CpG-site resolution DNA methylation analysis.","authors":"Guodong Zhao, Hui Li, Rui Zhang, Yanmiao Dai","doi":"10.1039/d6ay01922f","DOIUrl":"https://doi.org/10.1039/d6ay01922f","url":null,"abstract":"DNA methylation is an important epigenetic modification and has been widely used as a biomarker for disease diagnosis, particularly in cancer screening. However, conventional quantitative methylation-specific PCR (qMSP) often lacks single-CpG-site resolution because the methylation states of neighboring CpG sites within primer or probe binding regions can interfere with amplification efficiency. In this work, we report a novel Hairpin Competition qMSP (HC-qMSP) strategy for precise DNA methylation analysis at single-CpG-site resolution. In this method, degenerate bases were introduced at non-target CpG positions within the primer and probe binding regions to eliminate interference from adjacent CpG sites. Meanwhile, a specially designed hairpin primer was introduced so that the extension product forms a hairpin structure that competes with the reverse primer during amplification, enabling selective amplification of methylated templates while suppressing unmethylated sequences. Under optimized conditions, the HC-qMSP assay achieved a discrimination sensitivity down to 0.1%, and showed good linearity across a wide dynamic range. The method was further validated using colorectal cancer tissues and stool samples, successfully distinguishing cancer samples from normal controls. Owing to its simplicity, low cost, and compatibility with conventional real-time PCR platforms, HC-qMSP provides a promising approach for precise DNA methylation analysis and potential clinical diagnostic applications.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A pyrene-incorporated BODIPY-based colorimetric and fluorometric chemodosimeter for cyanide recognition and its bio-imaging application. 一种用于氰化物识别及其生物成像应用的含芘bodipy比色和荧光化学剂量计。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-16 DOI: 10.1039/d6ay02121b

S G Jebastin Andrews, P Idavathi Gunaseeli, R Poornima, S Benita Jeba Silviya, G Gifta Jerith, Chithiraivel Balakrishnan

{"title":"A pyrene-incorporated BODIPY-based colorimetric and fluorometric chemodosimeter for cyanide recognition and its bio-imaging application.","authors":"S G Jebastin Andrews, P Idavathi Gunaseeli, R Poornima, S Benita Jeba Silviya, G Gifta Jerith, Chithiraivel Balakrishnan","doi":"10.1039/d6ay02121b","DOIUrl":"https://doi.org/10.1039/d6ay02121b","url":null,"abstract":"A pyrene-incorporated BODIPY-based sensor (L1) was prepared as an efficient colorimetric and fluorometric chemodosimeter for detecting the cyanide anion in acetonitrile/HEPES buffer (5 mM, pH 7.2, 1 : 9 v/v) at room temperature. The probe L1 exhibited a distinct visual color change and selectively showed a fluorogenic \"turn-off\" response to cyanide ions in acetonitrile/HEPES buffer. Mechanistic investigations indicated that nucleophilic addition of cyanide at the α-position of the dicyanovinyl moiety inhibited the intramolecular charge transfer of L1 and induced significant shifts in absorption and emission. Various techniques, including optical spectroscopy, NMR titration, HRMS analysis, and theoretical simulations, were used to investigate L1's sensing mechanism. The presence of competing anions had minimal or no impact on this cyanide identification process. The probe achieved an outstanding detection limit of 4.33 × 10-8 M. Furthermore, L1-impregnated filter paper strips enabled a visual, colorimetric, strip-based test that shifted from dark yellow to colorless upon exposure to cyanide. In addition, a smartphone-based technique was developed to capture the color shift, showing immense promise for cyanide detection without the need for sophisticated equipment. The formulation of L1 into an INHIBIT logic gate for facilitating cyanide identification using electronic devices is one of the significant applications. More significantly, L1 has been effectively used for fluorescence bioimaging in HeLa cells and for detecting cyanide anions in food samples, serving as an affordable and selective fluorescent sensor.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sandwich ECL immunoassay system for tau protein detection using Ru(II)-AuNP signal probes integrated with copper nanoclusters. 利用Ru(II)-AuNP信号探针与铜纳米簇集成的三明治ECL免疫分析系统用于tau蛋白检测。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-15 DOI: 10.1039/d6ay01267a

Arathy B K, Merin K Abraham, Geneva Indongo, Greeshma Rajeevan, Dheya Mohammed Dhahir, Neena Haridas, Neethu Prakash S, Rajalekshmi R, Dr Sony George

{"title":"A sandwich ECL immunoassay system for tau protein detection using Ru(II)-AuNP signal probes integrated with copper nanoclusters.","authors":"Arathy B K, Merin K Abraham, Geneva Indongo, Greeshma Rajeevan, Dheya Mohammed Dhahir, Neena Haridas, Neethu Prakash S, Rajalekshmi R, Dr Sony George","doi":"10.1039/d6ay01267a","DOIUrl":"10.1039/d6ay01267a","url":null,"abstract":"Tau protein is an important biomarker which is associated with neurodegenerative disorders, particularly Alzheimer's disease, where abnormal tau aggregation and hyperphosphorylation contribute to neuronal dysfunction and cognitive decline. The sensitive and early detection of tau protein is therefore crucial for the diagnosis, prognosis and therapeutic monitoring of neurodegenerative diseases. Owing to its clinical significance, the development of rapid, highly sensitive and reliable analytical platforms for tau detection has gained considerable attention in biomedical diagnostics. In this work, a Ru(II) based homogeneous sandwich ECL immunosensor was developed for the turn-on detection of tau. A polyclonal antibody was conjugated with Ru(II)@AuNP, which served as the ECL signal probe, and further integrated with monoclonal antibody conjugated L-Cys-CuNC to construct the ECL immunoassay system. Upon the addition of tau protein, the ECL intensity was enhanced due to the specific antigen-antibody interaction. The probe is selective and sensitive towards tau with a limit of detection of 0.23 pg mL-1. Also, the probe is validated with spiked human serum samples with a recovery percentage of 90 to 104%.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8917-8928"},"PeriodicalIF":3.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148862628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Density separation-Raman spectroscopic quantification of microplastic-antibiotic co-contamination and transport in plastic-mulched agricultural soils. 密度分离-拉曼光谱定量分析覆膜农业土壤中微塑料-抗生素共污染及迁移。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-15 DOI: 10.1039/d6ay01492e

Jia Ren, Faeiza Buyong, Yapeng Wang, Yichao Li, Chay Tay Chia, Huifang Yang

{"title":"Density separation-Raman spectroscopic quantification of microplastic-antibiotic co-contamination and transport in plastic-mulched agricultural soils.","authors":"Jia Ren, Faeiza Buyong, Yapeng Wang, Yichao Li, Chay Tay Chia, Huifang Yang","doi":"10.1039/d6ay01492e","DOIUrl":"10.1039/d6ay01492e","url":null,"abstract":"Plastic-mulched soils contain mineral particles, organic matter and weathered polymer fragments that complicate the joint measurement of microplastics and co-occurring antibiotics. We developed a matrix-adaptive workflow combining sequential Fenton-enzymatic digestion, ZnCl2/NaI cascade density separation, silver-membrane collection, µ-Raman chemical imaging and isotope-dilution LC-MS/MS. Performance was evaluated with four agricultural soils, four mulch-relevant polymers, two particle-size fractions, pristine and aged surfaces, and tetracycline, ciprofloxacin and sulfamethoxazole. Across 2304 independent particle-spike observations, mean microplastic recovery was 93.72 ± 3.17% (95% confidence interval, 93.59-93.85%), within-laboratory relative standard deviations were 2.5-3.4%, and Raman classification accuracy was 96.25%. Antibiotic recovery across 432 observations was 95.31 ± 3.61%; matrix effects ranged from -14.2 to 5.3%, while matrix-matched calibration gave R2 values of 0.9995-0.9998. Pyrolysis GC-MS confirmed Raman-derived mass estimates (R2 = 0.9897), with a mean inter-platform bias of 0.022 mg. Aging increased XPS O/C ratios, AFM roughness and BET area and nearly doubled Langmuir capacities; aged polyamide reached 32.66 mg g-1 for tetracycline. In saturated columns, pristine polyethylene slightly advanced breakthrough, whereas aged polyethylene and polyamide shifted the 50% breakthrough point from 2.34 pore volumes without microplastics to 2.86 and 3.22 pore volumes, respectively. Factorial and mixed-effects analyses, multiplicity-controlled contrasts, grouped cross-validation and an uncertainty budget supported the method's cross-matrix robustness. The workflow provides a quantitative basis for linking analytical recovery, particle surface chemistry and contaminant migration in complex agricultural soils.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"9077-9088"},"PeriodicalIF":3.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autofluorescence-free detection of trypsin based on persistent luminescent nanospheres and gold nanoparticle aggregation. 基于持续发光纳米球和金纳米颗粒聚集的胰蛋白酶的自荧光检测。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-15 DOI: 10.1039/d6ay01515h

Yichen Li, Xuan Wu, Hancan Wang, Hongjiao Zhang

{"title":"Autofluorescence-free detection of trypsin based on persistent luminescent nanospheres and gold nanoparticle aggregation.","authors":"Yichen Li, Xuan Wu, Hancan Wang, Hongjiao Zhang","doi":"10.1039/d6ay01515h","DOIUrl":"10.1039/d6ay01515h","url":null,"abstract":"Trypsin (Try) is involved in various physiological processes in the human body, and its abnormal expression is associated with certain diseases. Therefore, its simple and accurate detection has important practical significance. Herein, a persistent luminescence sensing system is developed for autofluorescence-free detection of Try based on the blue emitting SiO2/Sr2MgSi2O7:Eu2+ (SSMSE) persistent luminescent nanospheres synthesized by the mSiO2 template method and gold nanoparticle (AuNP) aggregation. Try can specifically hydrolyze peptides in the detection system to produce peptide fragments that can induce the aggregation of AuNPs, which weakens the quenching effect of AuNPs and leads to a decrease in the quenching degree of persistent luminescence of SSMSE, achieving Try detection. The proposed detection system demonstrates a good linear relationship within the range of 1-60 µg L-1 with a low detection limit of 0.40 µg L-1 and the ability to detect Try in actual samples with a recovery rate of 91.2%-106%. The developed method has expanded the use of SSMSE and provides new ideas for Try detection, which has certain practical value.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"9089-9097"},"PeriodicalIF":3.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cobalt-doped spiky carbon nanoflower-based nanozyme for enhanced luminol-H₂O₂ chemiluminescence detection. 基于钴掺杂的尖刺碳纳米花纳米酶增强鲁米诺- h2o2化学发光检测。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-15 DOI: 10.1039/d6ay01423b

Yi-Jun Ma, Cheng-Long Shen, Chuang Xu, Dong Xue, Chang-Lin Shang, Xiao-Nan Lai, Run-Wei Song, Guang-Song Zheng, Qing Lou

引用次数: 0

A programmable aptamer-triggered B-PER/CRISPR cascade amplification platform for ultrasensitive detection of Staphylococcus aureus. 一种可编程适配体触发B-PER/CRISPR级联扩增平台，用于超灵敏检测金黄色葡萄球菌。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-15 DOI: 10.1039/d6ay01701k

Jialin Jiang, Yuan Peng, Wenjie Ma, He Zhang, Shaoqing Xiong, Xin Fu, Jinyan Li

{"title":"A programmable aptamer-triggered B-PER/CRISPR cascade amplification platform for ultrasensitive detection of Staphylococcus aureus.","authors":"Jialin Jiang, Yuan Peng, Wenjie Ma, He Zhang, Shaoqing Xiong, Xin Fu, Jinyan Li","doi":"10.1039/d6ay01701k","DOIUrl":"https://doi.org/10.1039/d6ay01701k","url":null,"abstract":"Rapid and sensitive detection of Staphylococcus aureus (S. aureus) is critical for food safety and public health. Herein, this work developed an ABC (aptamer-B-PER-CRISPR) cascade detection platform, where B-PER stands for bidirectional primer exchange reaction, that integrates aptamer-mediated target recognition, B-PER isothermal amplification, and CRISPR/Cas12a trans-cleavage for highly sensitive and specific detection of S. aureus. Upon target recognition, the trigger strand T is released from the aptamer-T duplex, initiating B-PER to generate abundant products containing sequences recognized by the guide RNA (gRNA), which activate Cas12a to cleave the reporter probe, generating a detectable fluorescence signal. Under optimized conditions, the platform exhibited a linear range of 102 to 1010 CFU per mL with a regression equation of F520 nm = 575.36 log C + 3246.47 (R2 = 0.9992) and a limit of detection of 13 CFU per mL. The assay showed excellent specificity for S. aureus, with negligible cross-reactivity toward Vibrio parahaemolyticus and Salmonella. Recovery tests in spiked milk samples yielded average recoveries of 98.9-100.7%. Overall, the ABC platform offers high sensitivity, excellent specificity, robust anti-interference capability, and operational simplicity, demonstrating great potential for rapid and reliable detection of foodborne pathogens in complex matrices.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Cu(0)-methylene blue clock reaction for sensitive Cr³⁺ detection in acidic media. 酸性介质中Cu(0)-亚甲基蓝时钟反应灵敏检测Cr3+。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-15 DOI: 10.1039/d6ay01052k

Decheng Zheng, Yajun Zheng, Yun Li, Yixin Hu, Meng Su, Ruiqin Mao, Zhiping Zhang

{"title":"A Cu(0)-methylene blue clock reaction for sensitive Cr3+ detection in acidic media.","authors":"Decheng Zheng, Yajun Zheng, Yun Li, Yixin Hu, Meng Su, Ruiqin Mao, Zhiping Zhang","doi":"10.1039/d6ay01052k","DOIUrl":"10.1039/d6ay01052k","url":null,"abstract":"Clock reactions, characterized by abrupt and time-controlled color transitions, are attractive platforms for analytical sensing, but methylene blue (MB)-based systems under acidic conditions typically rely on external reducing agents or nanostructured catalysts. Herein, we report a simple Cu(0) wire-catalyzed MB clock reaction that operates in acidic media (pH 2.0) without any added reducing agent or shape-controlled nanoparticles. The system exhibits reversible color transitions between MB and leuco-MB, governed by interfacial electron transfer and Cu redox cycling. Notably, at elevated MB concentrations (100 mg L-1), repeated clock cycles lead to the gradual formation of a brown precipitate, which can compromise analytical performance. Mechanistic investigations (SEM, EDS, and XPS) reveal that the precipitate originates from interactions among Cu species (Cu0/Cu+/Cu2+), Cl-, and MB, with partial incorporation of MB into CuCl-based solids during redox cycling. Strategies including acid cleaning of the Cu wire and lowering MB concentration effectively suppress precipitate formation and improve system stability. Under optimized conditions, the clock reaction enables quantitative detection of Cr3+via modulation of reaction kinetics, affording a linear response with a limit of detection of 3.22 µmol L-1. The method also demonstrates excellent selectivity and robustness in real water samples. This work establishes a reducing-agent-free MB clock platform and provides new insights into Cu-mediated interfacial redox processes, offering a simple, cost-effective, and practical strategy for metal ion sensing.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8950-8957"},"PeriodicalIF":3.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Red fluorescent europium metal-organic framework (Eu-MOF) integrated sensor strip on a 3D-printed device for smartphone-assisted in situ detection of picric acid. 用于智能手机辅助苦味酸原位检测的3d打印设备上的红色荧光铕金属有机框架（Eu-MOF）集成传感器条。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-15 DOI: 10.1039/d6ay01711h

Athira J Ajith, Yamuna Nair, Mekha Mariam Mathew, G Unnikrishnan, James Jenny, Simran Nagpal, Vineetha M C, T P Vinod

{"title":"Red fluorescent europium metal-organic framework (Eu-MOF) integrated sensor strip on a 3D-printed device for smartphone-assisted in situ detection of picric acid.","authors":"Athira J Ajith, Yamuna Nair, Mekha Mariam Mathew, G Unnikrishnan, James Jenny, Simran Nagpal, Vineetha M C, T P Vinod","doi":"10.1039/d6ay01711h","DOIUrl":"10.1039/d6ay01711h","url":null,"abstract":"This work focuses on the development of a portable ratiometric luminescent sensor device for the detection of picric acid (PA) in aqueous medium. The present study involves the fabrication of a europium MOF-PMMA polymer film into a 3D-printed device, engineered for red fluorescence emission. The probe (Eu-MOF/PMMA film) was thoroughly characterized using various spectroscopic and microscopic techniques, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS). The development of an analytical probe to monitor picric acid (PA), a highly mutagenic substance, is of enormous significance for the environment and health. The probe we developed exhibited strong red fluorescence at an excitation wavelength of 294 nm, which was selectively and sensitively quenched by picric acid. Additionally, the Eu-MOF/PMMA fluorescent strip exhibits a hypsochromic shift from red to green upon the addition of picric acid, making it a ratiometric fluorescent sensor for picric acid. The integration of smartphone-based Red-Green-Blue (RGB) analysis enables fast, portable, real-time detection of PA in real samples by quantifying color intensity variations. The PMMA matrix was deliberately chosen for the composite fabrication due to its excellent UV stability and ability to preserve optical transparency under UV irradiation, and the resulting luminescent detection device was found to be reusable, underscoring its potential as a portable, recyclable, and practically viable sensing platform. The device is easy to fabricate and cost-effective for the detection of picric acid in aqueous media, with a detection limit of 31.16 pM.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8958-8976"},"PeriodicalIF":3.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0