Analytical Methods最新文献

{"title":"Glycan profiling of multiple sclerosis oligoclonal bands with MALDI-TOF†","authors":"Furkan Şahin, Zelal Zuhal Kaya, Mustafa Serteser, Hasan Ümit Öztürk and Ahmet Tarık Baykal","doi":"10.1039/D4AY01639D","DOIUrl":"10.1039/D4AY01639D","url":null,"abstract":"<p >Multiple sclerosis (MS) is a common autoimmune disease that primarily affects young adults. In this condition, the immune system attacks the myelin sheath of nerve cells, leading to a variety of neurological symptoms. MS diagnosis often relies on the analysis of oligoclonal bands (OCBs), which involves detecting oligoclonal immunoglobulin G (IgG) bands in cerebrospinal fluid (CSF) and serum. The objective of this study was to investigate the glycosylation profiles of IgG in patients suspected of having MS, using glycan analysis with MALDI-TOF mass spectrometry. Serum samples were analysed, and the IgG glycosylation patterns were compared across different OCB types. Our findings suggest that alterations in IgG glycans may serve as potential biomarkers for MS, providing insights into the disease's molecular mechanisms and aiding in early diagnosis. This study highlights the importance of glycomics in understanding the pathogenesis of MS and in the development of novel diagnostic techniques.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 4","pages":" 850-858"},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A metal organic framework, UiO-66-NH2, based on a molybdenum Schiff base complex for the efficient electrochemical determination of diphenoxylate","authors":"Samira Saeednia, Masoud Rezaeinasab, Parvaneh Iranmanesh and Sobhan Abbasi Razgaleh","doi":"10.1039/D4AY01957A","DOIUrl":"10.1039/D4AY01957A","url":null,"abstract":"<p >Diphenoxylate, an agonist and opioid agent, is applied to enhance the activity of the circular muscle of the intestine. In this work, we prepared a novel electrochemical sensor for the determination of diphenoxylate based on a modified UiO-66-NH<small>₂</small> metal–organic framework (UiO-66-NH<small>₂</small> MOF) using a molybdenum Schiff base complex and NiS nanoparticles (NiSnp) in a carbon paste electrode (CPE). The UiO-66-NH<small>₂</small> MOF and UiO-66@Schiff-base-Mo were studied through advanced analysis techniques, such as X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDAX) and transmission electron microscopy (TEM), to reveal the inherent characteristics of the material. The results also showed that the morphology and structure of the UiO-66-NH<small>₂</small> MOF were maintained after surface modification. The electrochemical properties of the proposed modified electrode (NiSnp/MOF@Mo/CPE) were characterized using cyclic voltammetry (CV). Also, a differential pulse voltammetry (DPV) method was employed for diphenoxylate determination with NiSnp/MOF@Mo/CPE. A linear range was achieved from 1.0 to 55.0 μM and 65.0 to 125.0 μM, and the detection limit was found to be 0.34 μM. The capability of the fabricated sensors based on NiSnp/MOF@Mo/CPE was investigated through diphenoxylate determination in real samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 4","pages":" 762-771"},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enabling simultaneous photoluminescence spectroscopy and X-ray footprinting mass spectrometry to study protein conformation and interactions†","authors":"Sayan Gupta, Brandon Russell, Line G. Kristensen, James Tyler, Shawn M. Costello, Susan Marqusee, Behzad Rad and Corie Y. Ralston","doi":"10.1039/D4AY01670J","DOIUrl":"10.1039/D4AY01670J","url":null,"abstract":"<p >X-ray footprinting mass spectrometry (XFMS) is a structural biology method that uses broadband X-rays for <em>in situ</em> hydroxyl radical labeling to map protein interactions and conformation in solution. However, while XFMS alone provides important structural information on biomolecules, as we move into the era of the interactome, hybrid methods are becoming increasingly necessary to gain a comprehensive understanding of protein complexes and interactions. Toward this end, we report the development of the first synergetic application of inline and real-time fluorescent spectroscopy at the Advanced Light Source's XFMS facility to study local protein interactions and global conformational changes simultaneously. To facilitate general use, we designed a flexible and optimum system for producing high-quality spectroscopy-XFMS hybrid data, with rapid interchangeable liquid jet or capillary sample delivery for multimodal inline spectroscopy, and several choices for optofluidic environments. To validate the hybrid system, we used the covalently interacting SpyCatcher–SpyTag split protein system. We show that our hybrid system can be used to detect the interaction of SpyTag and SpyCatcher <em>via</em> fluorescence resonance energy transfer (FRET), while elucidating key structural features throughout the complex at the residue level <em>via</em> XFMS. Our results highlight the usefulness of hybrid method in providing binding and structural details to precisely engineer protein interactions.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 6","pages":" 1214-1225"},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining digital imaging and quantum dots for analytical purposes","authors":"João Paulo B. de Almeida, Thomas Fernando Ferreira Tributino dos Santos, José Roberto Sabino Júnior, Elias Vinicius Ferreira do Amaral, Claudinéia R. S. Oliveira, Matheus V. Maia, Willian T. Suarez, Lucas B. Ayres, Carlos D. Garcia and Vagner B. dos Santos","doi":"10.1039/D4AY02097A","DOIUrl":"10.1039/D4AY02097A","url":null,"abstract":"<p >This review provides a critical assessment of the most recent advances in digital imaging (DI) methods, applied for the development of analytical methodologies combining quantum dots (QDs). The state-of-the-art, treatment of data, instrumental considerations, software, sensing approaches, and optimization of the resulting methods are reported. Applications of the technology for the analysis of food and beverages, biomedically relevant analytes, drugs, environmental samples and forensic samples are also discussed. These examples aim to highlight the advantages of DI over traditional instrumentation, that in combination with QDs represents a powerful option for low-cost and on-site analyses. Moreover, some of these DI methods have been explored in the context of green chemistry principles, demonstrating a sustainable approach to modern analytical challenges.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 5","pages":" 916-938"},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical biosensor arrays based on nanozymes for environmental monitoring and food safety detection: principles, design, and applications","authors":"Wei Zhang, Jiao Chen and Ling Xie","doi":"10.1039/D4AY02088J","DOIUrl":"10.1039/D4AY02088J","url":null,"abstract":"<p >Typical biosensing platforms are based on the “lock-and-key” approach, providing high specificity and sensitivity for environmental and food safety monitoring. However, they are limited in their ability to detect multiple analytes simultaneously. With the use of pattern identification methods, biosensor arrays can detect faint fluctuations caused by multiple analytes with similar properties in complex systems. As a simple and efficient detection tool, optical biosensor arrays have become crucial for on-site and visible environmental and food safety monitoring. To enhance their practical applications, enzyme-like nanomaterial (nanozyme)-based biosensor arrays have been developed and integrated into optical biosensing platforms, leveraging their exposed active sites and tunable catalytic capabilities. For the development of an optical biosensor array, it is essential to incorporate multiple biosensing elements that can specifically interact with analytes to produce distinct “fingerprint” signals, enabling the differentiation of different targets <em>via</em> pattern identification. This review provides a comprehensive overview of nanozyme-based optical biosensor arrays for environmental and food safety monitoring. It explores the selective approaches of nanozyme-based colorimetric and fluorescent biosensor arrays, compares detection platforms utilizing nanozyme systems, and emphasizes the application of nanozyme-based optical biosensor arrays for environmental and food hazard monitoring. By evaluating current trends and summarizing both prospects and challenges, this review offers valuable guidance for the rational design of unique nanozyme-based optical biosensor arrays.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 5","pages":" 882-891"},"PeriodicalIF":2.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of alkyl-anthraquinone derivatives from hydrogen peroxide industrial process, using LC × LC-HRMS with shifting gradients†","authors":"Clémence Giffard, Florent Rouvière, Olivier Falletti, Béatrice Allard-Breton, Laurent Wendlinger, Jean-Michel Bossoutrot and Karine Faure","doi":"10.1039/D4AY01905A","DOIUrl":"10.1039/D4AY01905A","url":null,"abstract":"<p >The characterization of industrial working solutions containing numerous structurally related compounds and isomers requires the use of two-dimensional liquid chromatography coupled with high-resolution mass spectrometry (LC × LC-HRMS). The separation of alkyl-anthraquinone derivatives produced during hydrogen peroxide production was achieved by coupling a biphenyl and a C18 in the first and second dimensions, combined with the use of continuous shifting gradients in the second dimension (<small>²</small>D). The use of shifting gradients offers a significant advantage over regular gradients, with a 20% increase in occupancy and better separation of isomers eluted within the same modulation. Additionally, MS spectra were improved by reducing ion suppression in the source. The analysis of the industrial solutions produced a list of 226 peaks with 75 different molar masses, thereby confirming the presence of many isomers. The excellent repeatability of the retention times (within ±0.28 s in the second dimension) enabled the method to be used to study the differences between production batches, which were divided into two groups according to their measured hydrogen peroxide yield. Using multivariate statistical tools on the area of each peak, a total of 191 variables, including 11 clusters of insufficiently resolved isomers, were studied by principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). A simple Wilcoxon test performed on the most pertinent variables was able to confirm that 28 peaks could be used to monitor the industrial process. This work highlighted the efficacy of combining a very powerful separation technique with the implementation of two simple statistical tests to rapidly identity a set of discriminant markers in a highly complex industrial mixture.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 4","pages":" 742-751"},"PeriodicalIF":2.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A polyaniline-enhanced quartz crystal microbalance sensor for room-temperature camphor detection","authors":"Rizky Aflaha, Muammar Romiz Dzaki, Laila Katriani, Ahmad Hasan As'ari, Chlara Naren Maharani, Agus Kuncaka, Taufik Abdillah Natsir, Aditya Rianjanu, Ruchi Gupta, Kuwat Triyana and Roto Roto","doi":"10.1039/D4AY01859A","DOIUrl":"10.1039/D4AY01859A","url":null,"abstract":"<p >A method to detect camphor gas is considered indispensable in the pharmaceutical industry. Unfortunately, the available sensors to detect the presence of camphor in the air are very limited and still on a laboratory scale, such as using chromatography-mass spectroscopy (GC-MS). The research's main focus is to obtain a portable sensing system with excellent sensitivity and selectivity. This study explored polyaniline (PANi) concentrations cast over PVAc nanofiber as a matrix to detect camphor gas using a quartz crystal microbalance (QCM) system to measure camphor exposure. Scanning electron microscopy (SEM) and Fourier-transform infrared (FTIR) were used to analyze the morphology and chemical composition of the fabricated active layer (<em>i.e.</em>, nanofiber with PANi thin film). Increasing the PANi concentration provides more PANi on the sensor surface, thus amassing the active groups to interact with camphor gas molecules. It shows that a sensor with a 0.08% PANi thin film (Nano-PANi8) has a sensitivity of 2.594 Hz ppm<small>⁻¹</small>, much greater than the sensor without PANi, which is only 0.305 Hz ppm<small>⁻¹</small>. In addition, the sensor also has good repeatability and rapid response and recovery time of 47 s and 133 s, respectively. Compared to other gaseous compounds, the sensor also has excellent selectivity for camphor and robust long-term stability over three weeks of testing. The produced QCM sensor employing PANi thin film can give a camphor sensor superior performance, including excellent sensitivity, selectivity, and long-term stability. Furthermore, the use of QCM as a base sensor also makes the fabricated sensor portable.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 4","pages":" 724-734"},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-suppressed ion chromatography-tandem electrospray mass spectrometry using a short column for simultaneous analysis of dichloroacetic acid, trichloroacetic acid, and bromate: aqueous ammonia as the eluent additive†","authors":"Mingguo Peng, Jiali Chen, Shi Cheng, Huaicheng Zhang, Liangtao Pu, Erdeng Du, Qingfeng Cheng, Yanting Zuo and Aimin Li","doi":"10.1039/D4AY02007C","DOIUrl":"10.1039/D4AY02007C","url":null,"abstract":"<p >Dichloroacetic acid (DCAA), trichloroacetic acid (TCAA), and bromate (BrO<small>₃</small><small>⁻</small>) are disinfection byproducts (DBPs) formed during drinking water treatment and pose health risks. Rapid and reliable detection of these DBPs is essential for ensuring water safety. Non-suppressed ion chromatography (IC)-electrospray ionization mass spectrometry (IC-ESI-MS/MS) offers a promising approach for simultaneous analysis of organic haloacetic acids (HAAs) and inorganic oxyhalides, but previous methods using toxic methylamine can pose health risks. Herein, a new rapid non-suppressed IC-ESI-MS/MS method using a short AG18 guard column (13 μm, 2 mm × 50 mm) with aqueous ammonia as the alkaline modifier was developed for the simultaneous quantification of DCAA, TCAA, and BrO<small>₃</small><small>⁻</small>. This method allows direct sample injection without pretreatment and achieves sub-μg L<small>⁻¹</small> detection limits within 5 minutes. Aqueous ammonia (0.4 M) and acetonitrile were used as mobile phases in a gradient elution mode, providing good linearity (<em>R</em><small>²</small> &gt;0.995), low limits of quantification (0.48–1.13 μg L<small>⁻¹</small>), and high recoveries (76.3–107.3%). Intra-day (<em>n</em> = 9) and inter-day (3 days, <em>n</em> = 9) precision were validated with relative standard deviations of 5.8–8.9% and 6.4–7.7%, respectively. The method also demonstrated limited matrix interference from common water parameters such as Cl<small>⁻</small>, SO<small>₄</small><small>²⁻</small>, HCO<small>₃</small><small>⁻</small>/CO<small>₃</small><small>²⁻</small>, and natural organic matter (NOM), making it suitable for real water analysis. The applicability of this method was confirmed by monitoring DBP formation during the chlorination of NOM, showing that two-step chlorine dosing strategies reduce DBP formation compared to single-stage dosing. This newly established method provides a robust and efficient tool for DBP monitoring in drinking water treatment processes.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 4","pages":" 841-849"},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated magnetoimpedance biosensor microfluidic magnetic platform for the evaluation of the cardiac marker cTnI†","authors":"Zhen Yang, Jingyuan Chen, Mengyu Liu, Jiabao Huang, Jieping Liang, Mengjiao Zhu, Yuanwei Shen, Danqing Li, Chong Lei and Xuecheng Sun","doi":"10.1039/D4AY02021A","DOIUrl":"10.1039/D4AY02021A","url":null,"abstract":"<p >An integrated magnetoimpedance (MI) biosensor microfluidic magnetic platform was proposed for the evaluation of the cardiac marker, cardiac troponin I (cTnI). This bioanalyte evaluation platform mainly comprised three external permanent magnets (PMs), one MI element, two peelable SiO<small>₂</small> film units and a microfluidic chip (MFC). The MI element was made of micro-electro-mechanical system (MEMS)-based multilayered [Ti (6 nm)/FeNi (100 nm)]<small>₅</small>/Cu (400 nm)/[Ti (6 nm)/FeNi (100 nm)]<small>₅</small> thin films and designed as meander structures with closed magnetic flux. The MFC was fabricated using 3D printing and inverted molding techniques, designed with a solution by mixing the reaction region, magnetic separation region and detection region. Peelable SiO<small>₂</small> film units with the same size as the MI sensing element were used as the immunoreactivity interface of the bioanalytes. Two large PMs were placed directly below the MI sensing unit to provide a bias magnetic field, and the smaller PM was embedded in MCF for magnetic separation function. Different concentrations of the biological target (cTnI antigen)-, PBS buffer-, and Dynabeads-labeled polyclonal cTnI antibody solution were injected sequentially into the MCF. After immunoreactivity and magnetic separation, a classical sandwich immunoreaction process occurred on the surface of the monoclonal antibody-modified SiO<small>₂</small> film <em>via</em> self-assembling process in the reaction region of the MFC. The fundamental principle for evaluation of cTnI was based on variations of the MI signal under different concentrations of the biological target coupled with different numbers of Dynabeads. It was demonstrated that the mentioned MI-based magnetic platform could perform quantitative detection analyses over a range of cTnI concentrations (lowest concentration = 0.1 ng mL<small>⁻¹</small> and highest concentration = 100 ng mL<small>⁻¹</small>). The proposed MI-based magnetic platform provides a sensitive, reliable, stable and reusable bioanalytical platform, and it has potential in future biomedical applications.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 5","pages":" 990-998"},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Localized surface plasmon resonance sensing based on monometallic gold nanoparticles: from material preparation to detection of bioanalytes","authors":"Peng Zhang, Huizhen Hou, Songshi Xu, Yingfei Wen, Yonghui Zhang and Fei Xing","doi":"10.1039/D4AY01509F","DOIUrl":"10.1039/D4AY01509F","url":null,"abstract":"<p >The tunable geometrical properties of gold nanoparticles (AuNPs) endow them with the capacity to exhibit distinct behaviors with respect to both macroscopic (color) and microscopic (resonance wavelength) aspects, which has been extensively utilized in localized surface plasmon resonance (LSPR) sensing platforms. Additionally, functionalizing AuNP surfaces enhances the platforms' capabilities, allowing for the detection of a wide range of molecules related to various aspects of human health. In this review, we comprehensively elucidate the fundamental principles of LSPR biosensing and provide an in-depth survey of the preparation processes for metal nanoparticles, encompassing deposition technology for large-scale particle production as well as ion reduction methods that afford superior control over the particles' physical and chemical attributes. The sensing strategies based on adjustment of the dielectric environment and particle dispersion–aggregation levels are thoroughly reviewed and discussed. The discussion focused on a specific class of nanoparticles, characterized by their uniform shape and size, with each section bifurcated into two parts: a summary of the salient features and recent discoveries pertaining to the sensing strategy, as well as illustrations of representative, cutting-edge applications employing the strategy. We specifically aim to scrutinize analytes commonly encountered in the biomedical realm, encompassing biomarkers that serve as indicators of a wide range of diseases and microbial pathogens, while also prognosticating the future development trends of LSPR optical biosensor platforms within the biomedical field.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 5","pages":" 892-915"},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}