Mengmeng Zhang, Wei Zhou, Qingqing Wang, Ning Wang, Xin Wang, Youping Liu and Xin Di
{"title":"A water compatible magnetic molecularly imprinted nanocomposite for the class-selective enrichment of quinoxaline-1,4-dioxides in environmental water†","authors":"Mengmeng Zhang, Wei Zhou, Qingqing Wang, Ning Wang, Xin Wang, Youping Liu and Xin Di","doi":"10.1039/D4AY01250J","DOIUrl":"10.1039/D4AY01250J","url":null,"abstract":"<p >In this work, a novel water compatible core–shell magnetic molecularly imprinted nanocomposite (Fe<small><sub>3</sub></small>O<small><sub>4</sub></small>–COOH@MIPs) was synthesized in an acetonitrile–water reaction system by adopting carboxyl group-functionalized Fe<small><sub>3</sub></small>O<small><sub>4</sub></small> nanoparticles as the magnetic core, olaquindox as the template molecule and acrylamide as the functional monomer. The resulting Fe<small><sub>3</sub></small>O<small><sub>4</sub></small>–COOH@MIPs was characterized and applied as an adsorbent for magnetic solid-phase extraction (MSPE) of three quinoline-1,4-dioxides (QdNOs). The Fe<small><sub>3</sub></small>O<small><sub>4</sub></small>–COOH@MIPs possessed strong magnetic responsiveness, fast adsorption equilibrium rate (equilibrium within 5 min) and excellent selectivity. By coupling Fe<small><sub>3</sub></small>O<small><sub>4</sub></small>–COOH@MIPs based MSPE with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), good linearity (<em>R</em><small><sup>2</sup></small> ≥ 0.9985) and low limits of detection (0.83–5.0 ng L<small><sup>−1</sup></small>) for QdNOs were obtained. Finally, the proposed method was applied to determine trace QdNOs in environmental water samples, and the recoveries were in the range of 68.08–99.88% with RSD less than 12.5%.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 45","pages":" 7763-7771"},"PeriodicalIF":2.7,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ruixiao Zhang, Lin Zhang, Lvping Wu, Shengping You, Rongxin Su and Wei Qi
{"title":"Rapid detection of HPV16 utilizing recombinase polymerase amplification with the employment of an extremely low concentration of the probe†","authors":"Ruixiao Zhang, Lin Zhang, Lvping Wu, Shengping You, Rongxin Su and Wei Qi","doi":"10.1039/D4AY01625D","DOIUrl":"10.1039/D4AY01625D","url":null,"abstract":"<p >Human papillomavirus 16 (HPV16) infection is the leading cause of cervical cancer. The current mainstream method for detecting HPV16 is quantitative real-time PCR (qPCR). However, due to its time-consuming nature and reliance on expensive qPCR instruments, there is growing interest in more convenient, rapid and private approaches such as recombinase polymerase amplification (RPA). In this study, we innovated an effective method for HPV16 detection by integrating a RPA system with lateral flow strip (LFS) detection. Primers with optimal efficiency and specificity were designed, and false positive signals caused by dimers were eliminated by reducing the probe concentration. The RPA-LFS method demonstrates high sensitivity and specificity, capable of detecting 230 copies per reaction of HPV16 within 25 min without cross-reactivity to other subtypes. It exhibits good tolerance, remaining unaffected by 1.0% miconazole, 0.5% tioconazole and 1.0% hemoglobin. The results of clinical samples detected by this method were consistent with those of qPCR. The method provides a practical reference for HPV16 diagnosis and can be valuable in home and resource-limited settings, contributing to the reduction of cervical cancer incidence.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 44","pages":" 7557-7566"},"PeriodicalIF":2.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shan Liu, Yuemeng Chen, Fang Wang, Song Bai and JingJie Wu
{"title":"Analysis of synthetic route and case correlation of methamphetamine crystals seized by ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) and Pearson correlation coefficient method†","authors":"Shan Liu, Yuemeng Chen, Fang Wang, Song Bai and JingJie Wu","doi":"10.1039/D4AY01519C","DOIUrl":"10.1039/D4AY01519C","url":null,"abstract":"<p >Analysis of impurities in illicit drugs provides key information on the manufacture and distribution of methamphetamine (MA). An analysis of 22 specific target compounds in unlawful MA products was conducted using ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS). The peak area of the alkaloids was utilized as an important calculation data variable to determine the synthesis method. The results demonstrated that the application of target screening techniques for trace impurity components in samples can aid in determining the synthesis process routes for toxin production in 169 samples. These samples were initially categorized based on their synthesis modes, specifically synthesis routes <em>N</em>, <em>E</em>, and <em>R</em>. The correlation analysis of MA samples with known synthetic routes was carried out using principal coordinate analysis (PCoA) and the Pearson correlation coefficient method. Among the <em>N</em> and <em>R</em> synthesis routes, 15 and 7 groups with strong correlation were screened, respectively. Consistent with the observed scenario, the samples from the synthetic route <em>E</em> showed limited correlation with each other. The experiment provides a focused framework for the management of synthetic raw materials used for the production of drugs and the investigation of drug-related cases. This facilitates a holistic strategy to combat drug-related crimes, from manufacturing laboratory to end-user.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 44","pages":" 7509-7517"},"PeriodicalIF":2.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Highly sensitive electrochemical biosensor for MUC1 detection based on DNA-functionalized CdTe quantum dots as signal enhancers","authors":"JiaJia Wang, Chun Kan and Baokang Jin","doi":"10.1039/D4AY01544D","DOIUrl":"10.1039/D4AY01544D","url":null,"abstract":"<p >In this paper, an electrochemical biosensor based on a cadmium telluride/polypyrrole (CdTe/PPy) nanocomposite was developed for the detection of MUC1 with high selectivity and sensitivity. Results indicate that the CdTe/PPy nanocomposite is modified on the surface of the glassy carbon electrode (GCE), which affords a large surface area for immobilizing cap-DNA, ensuring its high selectivity and sensitivity. Next, CdTe-linked sig-DNA (MUC1 aptamer) was introduced, allowing the MUC1 aptamer to hybridize with cap-DNA. CdTe is a signal amplification element used to generate a differential pulse voltammetry (DPV) signal. Conceivably, target MUC1 detection was based on current signal change due to concentration change in the signal amplification element CdTe. In the presence of MUC1, the MUC1 aptamer specifically binds to MUC1, resulting in the release of CdTe-sig-DNA from the electrode surface and a decrease in peak current. Under optimized experimental conditions, the electrochemical biosensor is highly selective, sensitive, stable, and reproducible for MUC1 ranging from 0.1 nM to 100 nM with a detection limit of 0.05 nM (S/N = 3). Therefore, the electrochemical biosensor has potential applications in medical disease diagnosis.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 45","pages":" 7806-7815"},"PeriodicalIF":2.7,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junyu Zhang, Yi Chen, Yiyang He, Yiwen Bai, Wei Wang, Guangxin Yang, Cong Kong, Xiao Cao and Lin Gu
{"title":"Fruit waste-derived carbon dots with rhodamine B for the ratiometric detection of Fe3+ and Cu2+†","authors":"Junyu Zhang, Yi Chen, Yiyang He, Yiwen Bai, Wei Wang, Guangxin Yang, Cong Kong, Xiao Cao and Lin Gu","doi":"10.1039/D4AY01539H","DOIUrl":"10.1039/D4AY01539H","url":null,"abstract":"<p >A green and eco-friendly solvothermal approach is proposed for the synthesis of carbon quantum dots (CQDs) from watermelon rind. The as-prepared CQDs exhibited superior teal fluorescence in aqueous solutions, with a quantum yield of 13.9%. The CQDs and rhodamine B (RhB) were demonstrated to selectively react with Fe<small><sup>3+</sup></small> and Cu<small><sup>2+</sup></small>, leading to a fluorescence (FL) quenching effect, which was successfully used for constructing “double-response-off” type ratiometric FL probes. A comparative study was conducted to assess the sensitivity and accuracy of ratiometric fluorescent probes, specifically those based on CQDs alone and in combination with RhB, for the selective detection of Fe<small><sup>3+</sup></small> and Cu<small><sup>2+</sup></small>. By plotting the ratio of the differential fluorescence (Δ<em>F</em>) signals of CQDs to that of RhB against the practical application analyte concentration, the detection limits for Fe<small><sup>3+</sup></small> (1.75 μM) and Cu<small><sup>2+</sup></small> (0.43 μM) were markedly improved. The quenching mechanism was further explored, and the detection of Fe<small><sup>3+</sup></small> and Cu<small><sup>2+</sup></small> in surface water was demonstrated, showcasing the potential of efficient and effective nanosensors based on a static quenching effect. Futhermore, the addition of ascorbic acid can restore the fluorescence quenched by Fe<small><sup>3+</sup></small>. Therefore, in the presence of copper and iron, the ratiometric probe can demonstrate the ability to identify two different metals.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 43","pages":" 7413-7423"},"PeriodicalIF":2.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Label-free and ultrasensitive electrochemical detection of nucleic acids based on an exonuclease III-assisted target recycling amplification strategy using a heated gold disk electrode","authors":"Yanggang Cheng, Minglu Liu and Fangfang Wang","doi":"10.1039/D4AY01619J","DOIUrl":"10.1039/D4AY01619J","url":null,"abstract":"<p >The present work demonstrates a label-free, rapid and ultrasensitive electrochemical sensor for specific DNA detection with an exonuclease III (Exo III)-assisted target recycling amplification strategy and elevated electrode temperature at a heated gold disk electrode (HAuDE). The proposed electrochemical DNA (E-DNA) sensor was designed such that in the presence of the target DNA, the electrode self-assembled capture probe hybridizes with the target DNA to form a duplex structure, which triggers Exo III to specifically recognize this structure and selectively digest the capture probe, while the released target DNA underwent recycling to hybridize with a new capture probe, leading to the gradual digestion of a large amount of capture probes. It was found that during the digestion period, the activity of Exo III could be significantly improved by elevating electrode temperature, thus promoting the digestion reaction and improving the sensitivity for target DNA detection. Furthermore, an electrochemical indicator ([Ru(NH<small><sub>3</sub></small>)<small><sub>6</sub></small>]<small><sup>3+</sup></small>) was electrostatically bound to the capture probe, leading to a significant square wave voltammetry (SWV) response, which directly related to the amount and length of the capture probes remaining in the electrode and provided a quantitative measure for target DNA detection. The proposed strategy realized the highly sensitive detection of the target DNA with a detection limit of 26 aM (S/N = 3) at an electrode temperature of 40 °C during the digestion period, which was about two magnitudes lower than that at 24 °C.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 45","pages":" 7736-7743"},"PeriodicalIF":2.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Integrated instrumental setup comprising an automatic solution handling module and homemade luminometer with two photodetectors for the determination of antioxidants in wines","authors":"Rafael A. Alexandre and Boaventura F. Reis","doi":"10.1039/D4AY01597E","DOIUrl":"10.1039/D4AY01597E","url":null,"abstract":"<p >This study describes the development of an integrated instrumental setup, comprising a multi-commuted flow analysis module for solution handling and a homemade luminometer assembled with two photodetectors for luminescence detection. This setup controlled by an Arduino Due board was used to develop an analytical procedure for determining antioxidants in red and white wines, using chemiluminescence detection. The analytical procedure is based on the reaction of hypochlorite with antioxidants present in wine, followed of oxidizing reaction with luminol in an alkaline medium (pH > 9). After determining the optimal operational parameters, the following analytical parameters were obtained: linear responses for gallic acid concentrations ranging from 0.75 to 4.0 g L<small><sup>−1</sup></small> (<em>r</em><small><sup>2</sup></small> = 0.999) for red wine and from 75 to 500 mg L<small><sup>−1</sup></small> (<em>r</em><small><sup>2</sup></small> = 0.994) for white wine, a coefficient of variation of 2.71% (<em>n</em> = 9) for a wine sample with an antioxidant concentration of 1.57 g L<small><sup>−1</sup></small> in gallic acid equivalent, recoveries ranging from 85 to 114%, an analytical throughput of 100 determinations per hour, consumption of 0.35 μg of hypochlorite and 53 mg of luminol per determination, and limits of detection of 0.25 g L<small><sup>−1</sup></small> and 29 mg L<small><sup>−1</sup></small> for red and white wine, respectively.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 45","pages":" 7689-7698"},"PeriodicalIF":2.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guo-Ying Chen, Mao-Ling Luo, Li Chen, Jia-Li Wang, Tong-Qing Chai, Dan Wang and Feng-Qing Yang
{"title":"Correction: Selective fluorescence detection of acetylsalicylic acid, succinic acid and ascorbic acid based on a responsive lanthanide metal fluorescent coordination polymer","authors":"Guo-Ying Chen, Mao-Ling Luo, Li Chen, Jia-Li Wang, Tong-Qing Chai, Dan Wang and Feng-Qing Yang","doi":"10.1039/D4AY90110J","DOIUrl":"10.1039/D4AY90110J","url":null,"abstract":"<p >Correction for ‘Selective fluorescence detection of acetylsalicylic acid, succinic acid and ascorbic acid based on a responsive lanthanide metal fluorescent coordination polymer’ by Guo-Ying Chen <em>et al.</em>, <em>Anal. Methods</em>, 2024, <strong>16</strong>, 4981–4994, https://doi.org/10.1039/d4ay00696h.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 40","pages":" 6929-6929"},"PeriodicalIF":2.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/ay/d4ay90110j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shixin Cai, Mingrui Li, Xinqi Hu, Shuhua Gui, Menglu Li, Yuting Zhang, Xiaoli Wang and Nandi Zhou
{"title":"DNAzyme-mediated fluorescence signal variation of DNA-Ag nanoclusters and construction of an aptasensor for ATP†","authors":"Shixin Cai, Mingrui Li, Xinqi Hu, Shuhua Gui, Menglu Li, Yuting Zhang, Xiaoli Wang and Nandi Zhou","doi":"10.1039/D4AY01608D","DOIUrl":"10.1039/D4AY01608D","url":null,"abstract":"<p >DNA-templated silver nanoclusters (DNA-AgNCs) are novel nanomaterials with unique fluorescence characteristics. DNAzyme is a functional oligonucleotide that can catalyze the disruption of nucleic acid substrates. In this research, the effect of DNAzyme digestion on the fluorescence property of DNA-AgNCs was explored for the first time. A significant reduction in the fluorescence intensity of DNA-AgNCs after cleavage by DNAzyme was discovered. Further research found that the DNAzyme-catalyzed cleavage reduced the stability of DNA-AgNCs and led to their aggregation, accounting for a decline in fluorescence intensity up to 84%. Inspired by the above finding, a fluorescent aptasensor that integrates the benefits of DNA-AgNCs, exonuclease III (Exo III)-assisted signal amplification and DNAzyme was developed for sensitive detection of adenosine triphosphate (ATP). Under optimal conditions, the linear range was from 25 μM to 1000 μM and the detection limit was estimated to be 4.46 μM. Furthermore, this fluorescent aptasensor was effectively employed to quantify ATP levels in human serum samples, demonstrating its practicality in detecting ATP in biological matrices. The elucidation of DNAzyme-based fluorescence characteristic variation of DNA-AgNCs may provide insights into the interactions between DNAzyme and nanomaterials and has great prospects in the construction of fluorescent biosensors.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 45","pages":" 7676-7682"},"PeriodicalIF":2.7,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linna Song, Jianwen Han, Mingda Sui, Zihao Wei, Yunpeng Qin, Yuan Lu, Jiaojian Song, Wangquan Ye and Jinjia Guo
{"title":"Solid substrate assisted enhanced laser induced breakdown spectroscopy for metal element analysis in aqueous solution","authors":"Linna Song, Jianwen Han, Mingda Sui, Zihao Wei, Yunpeng Qin, Yuan Lu, Jiaojian Song, Wangquan Ye and Jinjia Guo","doi":"10.1039/D4AY01551G","DOIUrl":"10.1039/D4AY01551G","url":null,"abstract":"<p >Due to plasma quenching caused by the dense water medium, laser-induced breakdown spectroscopy (LIBS) faces challenges such as strong continuous background radiation and weak and broadened characteristic spectral lines when directly detecting metal elements in liquids. In this work, we introduced a simple approach to improve underwater LIBS signals with a solid substrate-assisted method, which requires no sample pre-treatment and simple operation and thus has potential for <em>in situ</em> marine applications. In this method, four submerged solid substrates (Zn, Cu, Ni, and Si) were employed to investigate the breakdown characteristics of underwater LIBS and the mechanism of spectral enhancement by using a CaCl<small><sub>2</sub></small> solution. The results demonstrated a significant improvement in the detection sensitivity of Ca with these substrates even at a short laser pulse with a relatively low laser energy (10 mJ). Among them, the semiconductor Si substrate exhibited the best enhancement effect, with an enhancement factor of over 75 for the Ca ionic lines at 393.4 nm and 396.8 nm and an enhancement factor of 29 for the Ca atomic line at 422.7 nm, respectively. This is mainly because the presence of substrate decreases the breakdown threshold of the liquid sample, and a higher plasma excitation temperature and electron density are obtained, which, in turn, leads to higher signal intensity. Furthermore, significant plasma emission enhancements for a wide range of elements are also achieved from seawater. These findings can contribute to the development of compact underwater <em>in situ</em> LIBS sensors with low power consumption, while ensuring high detection sensitivity.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 45","pages":" 7667-7675"},"PeriodicalIF":2.7,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}