Analytical Methods最新文献_第10页

Rapid and accurate detection of huanglongbing in citrus by elasticity testing using a piezoelectric finger†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/D4AY01952K

Pawan Rao, Shu Huang, Cheryl M. Armstrong, Joseph Capobianco, YongPing Duan, Wei-Heng Shih and Wan Y. Shih

{"title":"Rapid and accurate detection of huanglongbing in citrus by elasticity testing using a piezoelectric finger†","authors":"Pawan Rao, Shu Huang, Cheryl M. Armstrong, Joseph Capobianco, YongPing Duan, Wei-Heng Shih and Wan Y. Shih","doi":"10.1039/D4AY01952K","DOIUrl":"10.1039/D4AY01952K","url":null,"abstract":"Rapid and sensitive detection of citrus huanglongbing (HLB) is critical for the control of this devastating disease. In this study, we have evaluated using a piezoelectric finger (PEF) with a 0.4 mm probe to measure the elastic modulus of a leaf to detect HLB in four different species of citrus including grapefruit (GFT), pumelo (PUM), lemon (LEM), and Valencia orange (VAL). Diseased citrus leaves were harvested from trees testing positive for the presence of Candidatus Liberibacter asiaticus (Las), the causal agent of HLB, and included both symptomatic leaves, which were blotchy mottle or yellowing and asymptomatic leaves, which did not display outward symptoms. Healthy leaves were harvested from trees testing negative for Las. The results indicated that the PEF elastic modulus test exhibited an overall 94% sensitivity and 90% specificity against the Las status of the trees for all four citrus types combined. Comparative quantitative real-time polymerase chain reaction (qPCR) tests on the same leaves showed an overall 89% sensitivity and 100% specificity against the Las status of the trees. While a Cohen–Kappa coefficient of 0.81 was obtained between the PEF and qPCR predictions, suggesting a “strong” agreement between the PEF and qPCR tests, a more detailed examination indicated that PEF was more sensitive overall in detecting the Las positive trees than qPCR, particularly from asymptomatic leaves for which PEF was 96% sensitive versus 78% sensitive by qPCR, indicating the potential of using PEF for early detection of HLB.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2302-2311"},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d4ay01952k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-printed electrodes for electrochemical detection of environmental analytes 用于电化学检测环境分析物的 3D 打印电极。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/D4AY02271H

Liangliang Pan, Shijing Zhou, Jiaying Yang, Tongyun Fei, Shuduan Mao, Li Fu and Cheng-Te Lin

{"title":"3D-printed electrodes for electrochemical detection of environmental analytes","authors":"Liangliang Pan, Shijing Zhou, Jiaying Yang, Tongyun Fei, Shuduan Mao, Li Fu and Cheng-Te Lin","doi":"10.1039/D4AY02271H","DOIUrl":"10.1039/D4AY02271H","url":null,"abstract":"Environmental monitoring faces increasing demands for rapid, sensitive, and cost-effective analytical methods to detect various pollutants. Three-dimensional (3D) printing technology has emerged as a transformative approach for fabricating electrochemical sensors, offering unprecedented flexibility in electrode design and potential for customization. This comprehensive review examines recent advances in 3D-printed electrochemical sensors for environmental analysis, focusing on manufacturing technologies, materials development, and surface modification strategies. We analyze various printing approaches, including fused deposition modeling, stereolithography, and selective laser melting, discussing their relative advantages and limitations for electrode fabrication. The review explores conductive materials development, from carbon-based composites to novel metal-containing filaments, and examines crucial surface modification techniques that enhance sensor performance. Key applications in environmental monitoring are evaluated, including the detection of heavy metals, pathogens, antibiotics, and organophosphates, with particular attention to analytical performance metrics and real-world applicability. Technical challenges are critically assessed, including limitations in printing resolution, material conductivity, and long-term stability. The review concludes by identifying promising research directions, such as the integration of advanced materials and the development of automated manufacturing processes, highlighting opportunities for improving sensor performance and commercial viability in environmental monitoring applications.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2235-2253"},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Development of a novel UHPLC-UV combined with UHPLC-QTOF/MS fingerprint method for the comprehensive evaluation of Nao-Luo-Xin-Tong: multi-wavelength setting based on traditional Chinese medicinal prescription composition

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/D5AY90017D

Lina Wang, Yanling Wang, Guangyun Tong, Yu Li, Mengnan Lei, Huan Wu, Bin Wang and Rongfeng Hu

引用次数: 0

A fast, accurate strategy based on LAMP–NALF for detecting human adenovirus serotype 3 in children

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/D4AY01973C

Qi Yun, Wen Hui Xia, Si Fei Ma, Dong Ming Lu, Meng Gu and Ao Shuang Zhu

{"title":"A fast, accurate strategy based on LAMP–NALF for detecting human adenovirus serotype 3 in children","authors":"Qi Yun, Wen Hui Xia, Si Fei Ma, Dong Ming Lu, Meng Gu and Ao Shuang Zhu","doi":"10.1039/D4AY01973C","DOIUrl":"10.1039/D4AY01973C","url":null,"abstract":" Objective: To establish a rapid, convenient and accurate visual detection protocol for human adenovirus serotype 3 in children's respiratory infections based on LAMP–NALF. Methods: Adenovirus serotypes prevalent in the region (Changzhou City, Jiangsu Province, China) were analyzed by assessing the results of tNGS analysis of respiratory pathogens in children. On this basis, multiple sets of LAMP primers were designed for the adenovirus hexon gene as the detection target. Optimal primer combinations were further screened, and the composition of the reagent system and amplification procedures were improved to achieve efficient amplification. To realize an instrument-independent, fast-testing strategy, by combining NALF, which is based on the principle of lateral chromatography, small molecule antigens were modified for primers. Moreover, amplification products were bound to the corresponding antibodies on nucleic acid chromatography test strips for visualization. Finally, a series of tests were performed to validate the sensitivity, specificity, and methodological consistency of the LAMP–NALF assay. Results: Adenovirus infections in children in this region during this period were mainly caused by serotype 3. The optimal amplification temperature for optimal primers of LAMP was 65 °C. The combination of LAMP and NALF could detect femtogram-grade positive plasmid of the hexon gene; besides, there was no cross-reactivity with five other types of common respiratory pathogens in children. The agreement between the LAMP–NALF assay and tNGS/qPCR for detecting the adenovirus in 100 respiratory clinical specimens was 98%. Conclusion: The combination of LAMP and NALF can achieve rapid, convenient and accurate detection of human adenovirus type 3, which can provide a powerful support for the pathogenic diagnosis of adenoviral infections in children, thus contributing to the promotion of the development of point-of-care testing for pathogens.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2295-2301"},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Strategies for the detection of site-specific DNA methylation and its application, opportunities and challenges in the field of electrochemical biosensors

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/D5AY90024G

Chenliu Ye, Zhibin Zhao, Penghui Lai, Chunmei Chen, Fumei Jian, Haiying Liang and Qiongying Guo

引用次数: 0

A novel fluorescent probe for the detection of oxalyl chloride in air† 一种检测空气中草酰氯的新型荧光探针。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/D4AY02211D

Shuai Peng, Wenbin He, Shuyan Ye, Hao Zhang, Weiwei Zhang and Xianfeng Hou

引用次数: 0

Development and validation of a combined QuEChERS and HPLC-MS/MS method for trace analysis of ten diamide insecticides in agricultural products†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-19 DOI: 10.1039/D4AY02117G

Fajun Tian, Zhenzhen Zhou, Junfeng Lu, Chengkui Qiao, Caixia Wang, Tao Pang, Linlin Guo, Jun Li, Rongli Pang and Hanzhong Xie

{"title":"Development and validation of a combined QuEChERS and HPLC-MS/MS method for trace analysis of ten diamide insecticides in agricultural products†","authors":"Fajun Tian, Zhenzhen Zhou, Junfeng Lu, Chengkui Qiao, Caixia Wang, Tao Pang, Linlin Guo, Jun Li, Rongli Pang and Hanzhong Xie","doi":"10.1039/D4AY02117G","DOIUrl":"10.1039/D4AY02117G","url":null,"abstract":"Diamide insecticides are being widely registered worldwide, yet most of them lack established maximum residue limits (MRLs) in agricultural products. In this study, we combined a QuEChERS (quick, easy, cheap, efficient, rugged, and safe) extraction method with high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) analysis to simultaneously identify and quantify ten diamide insecticides in seven matrices for the first time. The method was validated in accordance with SANTE/11312/2021 guidelines, including sensitivity, linearity, trueness, and precision. Excellent linearity (R2 > 0.99) was obtained for all diamide insecticides within the concentration range of 5–1000 µg kg−1. The limit of detection (LOD) and limit of quantification (LOQ) were 0.01–1 µg kg−1 and 5 µg kg−1, respectively. The recoveries of the ten diamide insecticides at three levels (5, 100, and 1000 µg kg−1) ranged from 76.6% to 108.2% with good intra-day relative standard deviation (RSDr) (1.0–13.4%) and inter-day relative standard deviation (RSDR) (2.3–15.7%). The proposed method was applied to analyze 70 real agricultural product samples, and only six samples contained diamide insecticides. The results demonstrated that the method was both convenient and reliable for detecting diamide insecticides in agricultural products. The method was then applied to analyze agricultural product samples collected in a field trial to estimate the MRLs for the next step.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2282-2294"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d4ay02117g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: Application of new N- and S-doped amorphous carbon in D-μSPE and its combination with deep eutectic solvent-based DLLME for the extraction of some mycotoxins from soymilk

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-19 DOI: 10.1039/D3AY90109B

Anahid Rezaeefar, Mir Ali Farajzadeh, Mahboob Nemati, Mohammad Reza Afshar Mogaddam and Farzaneh Lotfipour

引用次数: 0

In situ sorbent formation dispersive solid phase extraction for multi-elemental analysis: a new approach using cyclen and a magnetic ionic liquid†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-19 DOI: 10.1039/D4AY01111B

Hasanain Najm Abed Altuwaijari, Mohammad Reza Afshar Mogaddam, Mir Ali Farajzadeh and Saeed Mohammad Sorouraddin

引用次数: 0

Disposable electrochemical biosensor based on acetylcholinesterase for inhibition assays using a natural substance and plant extracts†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-19 DOI: 10.1039/D4AY02084G

Sean dos Santos Araújo, Wilson Tiago Fonseca, Maria Fátima das Graças Fernandes da Silva, Moacir Rossi Forim, João Batista Fernandes and Ronaldo Censi Faria

{"title":"Disposable electrochemical biosensor based on acetylcholinesterase for inhibition assays using a natural substance and plant extracts†","authors":"Sean dos Santos Araújo, Wilson Tiago Fonseca, Maria Fátima das Graças Fernandes da Silva, Moacir Rossi Forim, João Batista Fernandes and Ronaldo Censi Faria","doi":"10.1039/D4AY02084G","DOIUrl":"10.1039/D4AY02084G","url":null,"abstract":"In general, insects are considered pests in agricultural areas, and their control is essential for high productivity of the cultivated areas. Control of these insects can be achieved by the inhibition of enzymes present in the insect's body. The enzyme acetylcholinesterase (AChE) is present in the neuromuscular junctions of vertebrates and invertebrates, and it is an important target for pest control. Herein, we present the development of a disposable electrochemical biosensor based on AChE (Bio-AChE) to rapidly evaluate the presence of new potential inhibitors in crude extracts of plants. Bio-AChE was constructed by modifying the disposable screen-printed carbon electrode (SPCE) with glutathione-decorated gold nanoparticles on which AChE was covalently immobilized. Electrochemical studies confirm the effective immobilization of the enzyme, and the Bio-AChE was applied to assess the inhibitory activity of azadirachtin, obtained from Azadirachta indica, which is a well-known AChE inhibitor. The proposed biosensor showed excellent results, demonstrating the inhibition activity of azadirachtin against AChE. The crude extracts of Picramnia riedelli, P. ciliata, and Toona ciliata were evaluated with the Bio-AChE, and all showed inhibition percentage values of around 50%. The extracts were evaluated by 1H NMR spectra, which identified classes of natural compounds that could be responsible for the inhibition activity. The proposed disposable Bio-AChE was shown to be a reliable method for simple and rapid screening of new inhibitors in plant extracts, opening an avenue for the screening of new natural products with potential for pest control.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 11","pages":" 2417-2426"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0