Analytical Methods最新文献

{"title":"Fluorescence and colorimetric analysis of β-estradiol based on aptamer assembled spherical nucleic acids†","authors":"Leyuan Chen, Aijiao Yuan, Dapeng Zhang, Wenjing Xie and Hanyong Peng","doi":"10.1039/D4AY01283F","DOIUrl":"10.1039/D4AY01283F","url":null,"abstract":"<p >Detecting β-estradiol (E2) in environmental monitoring is a complex task due to its status as a significant environmental contaminant. The detection methods require precision, sensitivity, and the ability to be conducted on-site without expensive instrumentation. Herein, we developed a novel approach using E2 aptamer assembled spherical nucleic acids (SNAs), which combines the sensitivity of fluorescence and the simplicity of colorimetry. Initially, a fluorescein (FAM)-labeled DNA aptamer is attached to the surface of gold nanoparticles (AuNPs) through hybridization with thiol-labeled DNA, resulting in fluorescence quenching. However, when E2 is present, the aptamer specifically binds to it, displacing from the thiol-DNA and releasing from the AuNP's surface. This leads to the recovery of fluorescence, allowing for quantitative detection of E2 by measuring the increase in fluorescence signal. Additionally, E2 detection can also be achieved visually using ultraviolet light. For colorimetric analysis, we introduce another set of AuNPs modified with thiol-DNA complementary to the DNA remaining on the surface of the previous AuNPs. When E2 triggers the release of the aptamer, the DNA on both AuNPs hybridized to each other, causing the aggregation of AuNPs and resulting in a distinct color change from red to purple. The detection limits for fluorescence and colorimetric analyses are 1 nM and 5 nM, respectively. We successfully applied this biosensing strategy to determine E2 concentrations in tap water and serum samples. Furthermore, our assay exhibits high selectivity towards E2 over other estrogens. Overall, this innovative approach provides an effective and versatile method for convenient on-site monitoring of E2.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/ay/d4ay01283f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142102269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Fluorescence intensity coded DNA frameworks based on the FRET effect enable multiplexed miRNA imaging in living cells","authors":"Xiaoshuang Zhao, Yi Xu and Xianqiang Mi","doi":"10.1039/D4AY90111H","DOIUrl":"10.1039/D4AY90111H","url":null,"abstract":"<p >Correction for ‘Fluorescence intensity coded DNA frameworks based on the FRET effect enable multiplexed miRNA imaging in living cells’ by Xiaoshuang Zhao <em>et al.</em>, <em>Anal. Methods</em>, 2023, <strong>15</strong>, 3051–3056, https://doi.org/10.1039/D3AY00578J.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/ay/d4ay90111h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A recognition-induced three-dimensional bipedal DNA walker for highly sensitive detection of APE1†","authors":"Qingyi Liu, Qiongdan Zhang, Yuting Zhang, Fanghong Tian, Kang Long, Yupei Yang, Wei Wang, Caiyun Peng and Huizhen Wang","doi":"10.1039/D4AY01353K","DOIUrl":"10.1039/D4AY01353K","url":null,"abstract":"<p >In contrast to the unipedal DNA walker, a bipedal DNA walker features a broader walking area and exhibits faster walking kinetics, leading to enhanced amplification efficiency. In this study, we designed a stochastic three-dimensional (3D) bipedal DNA walker, capable of navigating AuNP-based 3D tracks, driven by exonuclease III (Exo III). This detection system enables the linear detection of the non-invasive biomarker apurinic/apyrimidinic endonuclease 1 (APE1) activity across a range of 0 to 120 U per mL, with a detection limit of 0.03 U per mL. The platform not only offers a novel DNA walker for sensitive APE1 detection in cell lysate but also facilitates the precise assessment of NCA's inhibitory effect on APE1. This research holds promise for future screening of other potential APE1 inhibitors.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142078486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Greenness assessment of analytical methods for determination of cannabinoids in oils using NEMI, Analytical Eco-Scale, AGREE and GAPI","authors":"Nicolette Sammut Bartolo, Lovely L. Gallo, Karolina Szyrner, Paul I. Buhagiar and Janis Vella Szijj","doi":"10.1039/D4AY01083C","DOIUrl":"10.1039/D4AY01083C","url":null,"abstract":"<p >The cannabis plant is being increasingly researched due to its numerous therapeutic properties leading to the need for analytical techniques to assess substances present in extracts of the cannabis plant in carrier oils, such as medium chain triglycerides (MCT) oil. Awareness of the environmental impact of activities related to analysis led to the development of greenness assessment metrics. This study aimed to assess the environmental impact of analytical techniques applied in the analysis of cannabinoids in oil using Green Analytical Chemistry metrics. The first phase of the study consisted of a systematic literature review to identify high performance liquid chromatography and ultra high performance liquid chromatographic methods of analysis for cannabinoids in oil. In the second phase, the identified methods were assessed using the National Environmental Method Index (NEMI), Analytical Eco-scale, Analytical Greenness Calculator (AGREE) and Green Analytical Procedure Index (GAPI). Out of 124 identified studies, 8 were considered for the comparative analysis. The identified analytical methods consisted of high performance liquid chromatography (HPLC) using high resolution MS (<em>n</em> = 1), DAD (<em>n</em> = 2), UV (<em>n</em> = 1), UV and MS (<em>n</em> = 2) and MS/MS (<em>n</em> = 2) as detectors. When the analytical methods were assessed using the Analytical Eco-Scale, 7 out of 8 methods achieved a score ranging between 50 and 73, categorising them as acceptable green methods of analysis. One method achieved a total score of 80, categorising the method as an excellent green analysis. The application of Green Analytical Chemistry and respective metrics during the development of analytical methods contributes towards a reduction in the environmental footprint which results from related activities.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cu-MOFs@AuPtNPs nanozyme-based immunosorbent assay for colorimetric detection of alpha-fetoprotein†","authors":"Sitian Tang, Juan Cai, Kai Zhou, Zhu Mei, Dongmei Huang, Ling Liu, Lunyu Yang, Dan Yin and Liyi Hu","doi":"10.1039/D4AY01410C","DOIUrl":"10.1039/D4AY01410C","url":null,"abstract":"<p >Accurate detection of tumor biomarkers in blood is crucial for diagnosing and treating tumor disease. In this study, a metal enzyme-linked immunosorbent assay (MeLISA) was fabricated for the ultrasensitive and naked-eye detection of tumor biomarker alpha-fetoprotein (AFP) in clinical serum samples. Herein, novel copper metal–organic frameworks and gold platinum nanoparticle composites (Cu-MOFs@AuPtNPs) were synthesized for the first time by an <em>in situ</em> method, which showed an enormous specific surface area and excellent peroxidase (POx) mimicking properties. Cu-MOFs@AuPtNPs linked with antibodies targeting AFP served as a signal nanoprobe to amplify the detection signal. Additionally, the specificity of MeLISA was significantly enhanced through a conventional antigen–antibody reaction and efficient blocking of non-specific sites with BSA. Under optimal conditions, the sandwich-type MeLISA exhibited a wide range from 0.001 to 1000 ng mL<small>⁻¹</small> (<em>R</em><small>²</small> = 0.997) and a low detection limit of 0.86 pg mL<small>⁻¹</small> (S/N = 3) with acceptable stability, selectivity, and reproducibility. It is noteworthy that the suggested MeLISA performed exceptionally well in detecting clinical serum samples, which were visible to the naked eye and did not require complex platforms. To sum up, the innovative MeLISA based on Cu-MOFs@AuPtNPs provides an alternative method for early cancer diagnosis, particularly in economically backward areas where simple diagnostic apparatus is extremely desirable.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142118385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and application of KCC-1@ZIF-8 for the solid extraction of tetracycline with high adsorption capacity†","authors":"Zhang Jingru, Chu Hongtao, Chen Jiaqi, Wang Qian, Qin Shili, Gao Lidi, Ma Wenhui, Li Jun, Zhao Ming and Zhang Weibing","doi":"10.1039/D4AY00399C","DOIUrl":"10.1039/D4AY00399C","url":null,"abstract":"<p >In this study, three different materials were prepared: dendritic fiber-type silica (KCC-1), zeolitic imidazolate framework-8 (ZIF-8), and a new composite material called KCC-1@ZIF-8. These materials were synthesized using microemulsion, stirring, and coating methods, respectively. The properties of the materials were characterized using various techniques such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), TGA and X-ray diffraction (XRD). The results showed that KCC-1@ZIF-8 exhibited a significant increase in the BET surface area and pore size compared to the individual components KCC-1 and ZIF-8. These improved properties of the composite material were beneficial for enhancing the adsorption capacity. The effects of initial concentrations, solution pH and reaction time on the adsorption capacity were investigated. The adsorption kinetics and isothermal data of ZIF-8 and KCC-1@ZIF-8 fitted well with pseudo-second-order and Langmuir isotherm models. The results of adsorption thermodynamics show that the adsorption process is spontaneous and endothermic. KCC-1@ZIF-8 exhibited a very high adsorption capacity (751.46 mg g<small>⁻¹</small>) at an initial TC hydrochloride concentration of 80 mg L<small>⁻¹</small> in an aqueous solution at 301.15 K, and the value was higher than that of ZIF-8 (549.80 mg g<small>⁻¹</small>) under the same conditions. KCC-1 exhibited a relatively lower capacity (37.860 mg g<small>⁻¹</small>). Based on these findings, KCC-1@ZIF-8 was considered a promising adsorbent for the treatment of wastewater contaminated with TC hydrochloride. Additionally, the composite material, when combined with high-performance liquid chromatography (HPLC), could be used as a solid-phase extraction adsorbent for the adsorption of TC hydrochloride in animal foodstuff samples. The calibration curves showed a linear range of 20–500 μg L<small>⁻¹</small>, and the recovery rate ranged from 85.216% to 90.717%. No one has made adsorbents with this new structure before, and KCC-1@ZIF-8 possessed excellent adsorption properties, which make it a potential candidate for environmental remediation and analytical applications involving TC hydrochloride.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141998936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Durability of the bubble-jet sorter enables high performance bio sample isolation†","authors":"Thomas Hopfes, Radin Tahvildari, Koen de Wijs, Chi Dang, Jelle Fondu, Liesbet Lagae and Sarah Libbrecht","doi":"10.1039/D4AY01168F","DOIUrl":"10.1039/D4AY01168F","url":null,"abstract":"<p >Sorting cells while maintaining their viability for further processing or analysis is an essential step in a variety of biological processes ranging from early diagnostics to cell therapy. Sorting techniques such as fluorescence-activated cell sorting (FACS) have evolved considerably and provide standard ways of sorting. Nevertheless, the search for compact, integrated, efficient, and high throughput microfluidic sorting platforms continues due to challenges such as cost, cell viability, and biosafety. In our previous work, we introduced a technology with the potential to become such a platform: the bubble-jet sorter. It is a silicon-based sorter chip relying on cell deflection through micro vapor bubble formation. In this work, we present a new version of the sorter chip that emphasizes durability and continuous sorting operation. To characterize the sorter, we first focus on the technical performance and show a sorter lifetime that repeatedly exceeds 80 million actuation cycles. In addition, we show continuous operation at high firing rates, but also discuss limitations due to heat buildup. In a second step, we present continuous sorting runs of millions of beads and CD3 positive T cells at rates surpassing 1000 sorting events per second, while maintaining high purity (&gt;90%) and recovery (&gt;85%). Dedicated viability tests show that the gentle sorting process maintains cell viability in this closed, aerosol-free device. The remarkable combination of high lifetime, sorting rate, and sorting efficiency, along with the potential for on-chip parallelization show the promise of this technology to meet the growing demand for large-scale sample isolation in drug and immunotherapy development.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a mix-and-read assay for human asprosin using antibody–oligonucleotide probes and thermofluorimetric analysis†","authors":"Juan Hu and Christopher J. Easley","doi":"10.1039/D3AY01175E","DOIUrl":"10.1039/D3AY01175E","url":null,"abstract":"<p >Adipose tissue, or fat tissue, can now be classified as an endocrine organ as it responds to stimuli by secreting a range of hormones, termed adipokines, which regulate the functions of various other tissues and organs. Because novel adipokines continue to be discovered and characterized by researchers, there is an enduring need for the development of new analytical assays that target these hormones. Discovered recently, asprosin is an adipokine hormone secreted by white adipose tissue (WAT) during fasting which has been implicated for its important effects on the liver, skeletal muscle, hypothalamus, pancreas, and possibly other tissues. While standard immunoassays have been developed, the continued surge in research on asprosin's function would greatly benefit from an assay with homogeneous, mix-and-read workflow, and the nanomolar clinical range makes this goal more feasible. In this work, we developed such an assay for asprosin using our thermofluorimetric analysis (TFA) methods with antibody–oligonucleotide conjugate probes. The assay, achievable in less than one hour, was successfully validated by quantifying native levels of asprosin in human serum collected from fasting, nonfasting, type II diabetic, and obese donors.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142015604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zn-MOF@rGO nanocomposite: a versatile tool for highly selective and sensitive detection of Pb2+ and Cu2+ ions in water†","authors":"Ajay Kumar, Kuldeep Kumar, Kirtanjot Kaur, Kushal Arya, Surinder Kumar Mehta, Surinder Singh and Ramesh Kataria","doi":"10.1039/D4AY00987H","DOIUrl":"10.1039/D4AY00987H","url":null,"abstract":"<p >In this work, a hybrid nanocomposite material (PUC2@rGO) was prepared by integrating our previously developed Zn-MOF (PUC2) with reduced graphene oxide (rGO) through the wet impregnation method. The characterization of PUC2@rGO was performed using various analytical techniques, including FTIR, PXRD, FE-SEM, HR-TEM, XPS, zeta potential, and time-resolved FL spectroscopy. Our investigation primarily focused on assessing the composite's capability to detect water pollutants. Notably, PUC2@rGO demonstrated remarkable selectivity and sensitivity towards Pb<small>²⁺</small> and Cu<small>²⁺</small> ions <em>via</em> fluorescence quenching, exhibiting low detection limits and high quenching constant values. Spectroscopic analysis revealed that electron transfer from PUC2@rGO (donor) to the metal ions (acceptor) resulted in the observed quenching effect induced by Pb<small>²⁺</small> and Cu<small>²⁺</small> ions. Time-resolved fluorescence studies of PUC2@rGO before and after adding Pb<small>²⁺</small> and Cu<small>²⁺</small> ions confirmed dynamic quenching, further affirming strong interactions between PUC2@rGO and the targeted metal ions. These findings highlight PUC2@rGO's potential for efficiently detecting heavy metal pollutants in water.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flexible and wearable electronic systems based on 2D hydrogel composites","authors":"Sushil Kumar Verma, Varee Tyagi, Sonika, Taposhree Dutta and Satyendra Kumar Mishra","doi":"10.1039/D4AY01124D","DOIUrl":"10.1039/D4AY01124D","url":null,"abstract":"<p >Flexible electronics is a rapidly developing field of study, which integrates many other fields, including materials science, biology, chemistry, physics, and electrical engineering. Despite their vast potential, the widespread utilization of flexible electronics is hindered by several constraints, including elevated Young's modulus, inadequate biocompatibility, and diminished responsiveness. Therefore, it is necessary to develop innovative materials aimed at overcoming these hurdles and catalysing their practical implementation. In these materials, hydrogels are particularly promising owing to their three-dimensional crosslinked hydrated polymer networks and exceptional properties, positioning them as leading candidates for the development of future flexible electronics.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142102268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}